Culture dish for researching selectivity of insects to in-vitro tobacco tissues

Abstract

The utility model discloses a culture dish for researching the selectivity of insects to in-vitro tobacco tissues, and relates to the technical field of insect behavior research. The culture dish comprises a culture dish body and a culture dish cover, and the culture dish cover is connected to the culture dish body in a covering manner; a sample chamber, an insect movement channel and an insect sample adding chamber are arranged in the culture dish body; the insect sample adding chamber is positioned in the middle of the culture dish body; one end of the insect movement channel is communicated with the insect sample adding chamber, and the other end of the insect movement channel is communicated with the sample chamber. According to the utility model, the culture dish is partitioned, and each partition structure is arranged, so that the problems that the interaction relationship between a plurality of insects and a plurality of samples can not be researched by the existing device, and the reasons for selecting the samples by the insects can not be deeply researched are effectively solved.

Description

Technical Field

[0001] The utility model relates to the technical field of insect behavior research, in particular to a culture dish for studying the selectivity of insects to excised tobacco tissues. Background Art

[0002] Research shows that tobacco attracts insects by releasing chemical substances and other means, and insects interact with tobacco through parasitism and other means. Studying the preference of insects for tobacco can more accurately study the corresponding insect-resistant devices or drugs. According to the characteristics of the interaction between insects and plants, designing a reasonable and easy-to-operate experimental device is a necessary condition for the success of the research.

[0003] Regarding the research on the interaction between insects and tobacco, the focus has mostly been on the preference of different species of insects for tobacco, and there has been little research on the preference of insects for different parts of tobacco. Biological insect experiments generally occur in tobacco fields, but field experiments are greatly affected by the environment, and insects shuttle among tobacco plants, making it difficult to monitor their dynamics. Therefore, it is simpler and more convenient to conduct research on insect preference on excised plants. Currently, devices for studying the interaction between insects and excised plants, such as "a culture dish and a culture dish assembly for studying the interaction between plants and insects" (Chinese utility model, authorization publication number: CN 211932149 U, publication date: November 17, 2020), can only study the interaction between a single insect and a single sample in an independent space, and errors are likely to occur in the test results of multiple insects and multiple samples. Therefore, how to provide a device that is easy to observe and can simultaneously study the preference of multiple insects for multiple samples has become an urgent problem for those skilled in the art. Summary of the Invention

[0004] The purpose of the utility model is to provide a culture dish for studying the selectivity of insects to excised tobacco tissues, which solves the problems that the existing devices cannot study the interaction between multiple insects and multiple samples, and cannot deeply study the reasons for the sample selection by insects.

[0005] To solve the above technical problems, the utility model adopts the following technical solutions:

[0006] The utility model provides a culture dish for studying the selectivity of insects to excised tobacco tissues, which includes a culture dish body and a culture dish cover, and the culture dish cover is covered and connected to the culture dish body; a sample chamber, an insect movement channel and an insect loading chamber are arranged in the culture dish body;

[0007] The insect loading chamber is located in the middle of the culture dish body; the insect movement channels are arranged in a radial pattern, one end of the insect movement channel communicates with the insect loading chamber, and the other end communicates with the sample chamber.

[0008] Preferably, the culture dish cover is provided with ventilation holes and an insect sampling port;

[0009] The ventilation holes correspond to the positions of the sample chambers one by one.

[0010] Preferably, the insect sampling port is equipped with a lid.

[0011] Preferably, the culture dish body and the culture dish cover are made of transparent glass or plastic.

[0012] Preferably, the sample chamber is arranged in a hemispherical shape.

[0013] Preferably, the cross-section of the insect movement channel is semi-cylindrical, and the radius of the insect movement channel is smaller than the radius of the sample chamber.

[0014] Preferably, the insect loading chamber is arranged in a hemispherical shape.

[0015] Preferably, the number of the sample chambers is 3 to 6 and they are evenly distributed in a circle.

[0016] Preferably, the culture dish cover is snap-fitted or threadedly connected to the top of the culture dish body.

[0017] Compared with the prior art, the beneficial technical effects of the present utility model are as follows:

[0018] A culture dish for studying the selectivity of insects to in vitro tobacco tissues provided by the present utility model effectively solves the problems that the existing devices cannot study the interaction relationship between multiple insects and multiple samples, and cannot deeply study the reasons for the selection of samples by insects by setting partitions for the culture dish and setting the structures of each partition. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The present utility model will be further described below with reference to the accompanying drawings.

[0020] Figure 1 It is a top view of the culture dish body of the present utility model;

[0021] Figure 2 It is a top view of the culture dish cover of the present utility model;

[0022] Figure 3 It is a schematic cross-sectional view of the culture dish body of the present utility model;

[0023] Figure 4 It is a schematic diagram of the overall structure of the assembled culture dish of the present utility model.

[0024] Explanation of reference numerals: 1. Culture dish body; 2. Sample chamber; 3. Insect movement channel; 4. Insect loading chamber; 5. Culture dish cover; 6. Ventilation hole; 7. Insect sampling port; 8. Lid. Detailed implementation mode

[0025] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present utility model clearer and more understandable, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present utility model and are not used to limit the present utility model.

[0026] As Figures 1 to 4 shown, a culture dish for studying the selectivity of insects to in vitro tobacco tissues includes a culture dish body 1 and a culture dish cover 5, and the culture dish cover 5 is covered and connected to the culture dish body 1; a sample chamber 2, an insect movement channel 3 and an insect sample addition chamber 4 are arranged in the culture dish body 1.

[0027] Specifically, the insect sample addition chamber 4 is located in the middle of the culture dish body 1; the insect movement channels 3 are arranged in a radial pattern, one end of the insect movement channel 3 communicates with the insect sample addition chamber 4, and the other end communicates with the sample chamber 2. The insect movement channels separate the insect sample addition chamber and the sample chamber, which can not only visually observe the selection of insects, but also prevent insects from flying randomly in the culture dish and unable to capture their movement trajectories. The radius of the insect movement channels can be adjusted adaptively according to the sizes of different insects.

[0028] Specifically, ventilation holes 6 and an insect sample inlet 7 are provided on the culture dish cover 5.

[0029] Specifically, the ventilation holes 6 correspond to the positions of the sample chamber 2 one by one, which can supply sufficient oxygen to the insects, avoid the death of insects due to lack of oxygen, and ensure the normal progress of the experiment.

[0030] Specifically, a lid 8 is equipped on the insect sample inlet 7 。

[0031] Specifically, the materials of the culture dish body 1 and the culture dish cover 5 are transparent glass. In other implementation modes, the materials of the culture dish body 1 and the culture dish cover 5 can also be transparent plastic, which is convenient for naked-eye observation.

[0032] Specifically, the sample chamber 2 is set to be hemispherical. When used for studying the interaction relationship between in vitro tobacco tissues and insects, the hemispherical sample chamber can ensure that whether it is a solid sample or a liquid sample, it will not move to other areas and affect the selection of insects. Therefore, the present utility model has no limitation on the morphology of the tobacco tissue sample, and it can be an in vitro tissue, a tissue extract or other morphological samples. Therefore, the present utility model can also be used to assist in studying the reasons for the preference of insects for tobacco.

[0033] Specifically, the cross-section of the insect movement channel 3 is semi-cylindrical, and the radius of the insect movement channel 3 is smaller than the radius of the sample chamber 2.

[0034] Specifically, the insect loading chamber 4 is provided in a hemispherical shape. The structure of the insect loading chamber enlarges the initial activity space of the insects, can accommodate various insects, and avoids the random flying and bumping of the insects due to the narrow space when entering the experimental device, which may affect the experimental results.

[0035] Specifically, the number of the sample chambers 2 is 4 and they are evenly distributed in a circle. In other embodiments, the number of the sample chambers 2 can also be 3, 5 or 6, and can be adjusted according to actual needs.

[0036] Specifically, the culture dish cover 5 is snap-connected to the top of the culture dish body 1, which can form a closed space to prevent the insects from escaping. In other embodiments, the culture dish cover 5 can also be connected to the top of the culture dish body 1 by threads.

[0037] In specific implementation, those skilled in the art can also specifically adjust the size of the above-mentioned culture dish according to actual needs.

[0038] The using process of the present utility model is as follows:

[0039] First, add tobacco samples to the sample chambers on the culture dish body.

[0040] Then, assemble the culture dish body 1 and the culture dish cover 5. Open the lid 8, put the insects into the insect inlet 7, and then cover the lid 8 to prevent the insects from escaping. After the insects enter the insect loading chamber 4, they move along the insect movement channel 3 and finally stay in the sample chamber 2. After that, the activities of the insects in the culture dish can be observed with the naked eye, or the activities of the insects can be recorded by using a monitoring camera.

[0041] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprising", "including" or any other variant thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes other elements not expressly listed, or also includes elements inherent to such process, method, article or device.

[0042] The above-described embodiments are only descriptions of the preferred modes of the present utility model, and do not limit the scope of the present utility model. Without departing from the design spirit of the present utility model, various deformations and improvements made by those of ordinary skill in the art to the technical solutions of the present utility model shall fall within the protection scope determined by the claims of the present utility model.

Claims

1. A culture dish for studying insect selectivity for isolated tobacco tissue, comprising a culture dish body (1) and a culture dish cover (5), wherein the culture dish cover (5) is connected to the culture dish body (1); characterized in that: The culture dish body (1) is provided with a sample chamber (2), an insect movement channel (3) and an insect sample loading chamber (4); The insect sample loading chamber (4) is located in the middle of the culture dish body (1); the insect movement channels (3) are arranged radially, one end of the insect movement channels (3) is connected to the insect sample loading chamber (4), and the other end is connected to the sample chamber (2).

2. The culture dish for studying insect selectivity for isolated tobacco tissue according to claim 1, characterized in that: The culture dish cover (5) is provided with an air vent (6) and an insect sampling port (7); The positions of the air holes (6) and the sample chambers (2) correspond one to one.

3. The culture dish for studying insect selectivity for isolated tobacco tissue according to claim 2, characterized in that: The insect inlet (7) is provided with a cover (8) 。 4. The culture dish for studying insect selectivity for isolated tobacco tissue according to claim 1, characterized in that: The culture dish body (1) and the culture dish cover (5) are made of transparent glass or plastic.

5. The culture dish for studying insect selectivity for isolated tobacco tissue according to claim 1, characterized in that: The sample chamber (2) is configured to be hemispherical.

6. The culture dish for studying insect selectivity for isolated tobacco tissue according to claim 1, characterized in that: The cross section of the insect movement channel (3) is semi-cylindrical, and the radius of the insect movement channel (3) is smaller than the radius of the sample chamber (2).

7. The culture dish for studying insect selectivity for isolated tobacco tissue according to claim 1, characterized in that: The insect sample loading chamber (4) is configured to be hemispherical.

8. The culture dish for studying insect selectivity for isolated tobacco tissue according to claim 1, characterized in that: The number of the sample chambers (2) is 3 to 6 and they are evenly distributed around the circumference.

9. The culture dish for studying insect selectivity for isolated tobacco tissue according to claim 1, characterized in that: The culture dish cover (5) is snap-fitted or threadedly connected to the top of the culture dish body (1).

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