Microfluidic device capable of realizing illumination culture

Abstract

The utility model relates to a micro-fluidic device capable of realizing illumination culture, which comprises a sample introduction system, a micro-fluidic chip, a culture pipeline, a liquid drop identification system, a liquid drop detection system, a control system and an illumination system, the sample introduction system is used for introducing a water phase and an oil phase into the micro-fluidic device capable of realizing illumination culture; the illumination system comprises a base plate, a coil pipe frame, an illumination lamp, a light guide column and a light blocking cover, the base plate is connected with the coil pipe frame, the illumination lamp is installed at the other end of the coil pipe frame, the light guide column is placed on the inner side of the coil pipe frame, and the light blocking cover is installed on the outer side of the coil pipe frame; according to the utility model, the air-permeable culture pipeline is selected, so that air exchange in the algae growth process can be met, the culture pipeline is wound on the coil pipe frame and then is wrapped outside by the light blocking cover to form the closed space, and the illumination requirements of different algae culture are met by adjusting the light intensity in the closed space.

Description

Technical Field

[0001] The utility model relates to the field of biological instruments, in particular to a microfluidic device capable of light cultivation. Background Art

[0002] At present, the general structure of a photobioreactor for culturing algal microorganisms is as follows: a tubular photobioreactor is made of glass material or acrylic with good light transmittance, and air holes are added in the pipeline to meet the gas exchange during the algal cultivation process. A light source is introduced outside or at the bottom of the pipeline, and an external water pump or peristaltic pump is used as the power source to make the algae in a circulating motion state in the pipeline. This solution has the following disadvantages: (1) It is impossible to perform real-time detection and record the growth situation during the algal cultivation process; (2) When subculture is carried out, the reagent consumption is large; (3) In order to meet the gas exchange requirements during the cultivation process, holes need to be opened in the cultivation pipeline, which has the risk of being polluted by the external environment. Content of the Utility Model

[0003] In order to solve the above problems, the utility model provides a microfluidic device capable of light cultivation, which includes a sampling system, a microfluidic chip, a cultivation pipeline, a droplet identification system, a droplet detection system, a control system and a lighting system. The sampling system is used to introduce an aqueous phase and an oil phase into the device to form micro-scale water-in-oil droplets. It is characterized in that the lighting system includes a chassis, a coil pipe rack, a lighting lamp, a light guide column and a light shield. The coil pipe rack is fixedly connected to the chassis, the lighting lamp is fixedly connected to the other end of the coil pipe rack, the light guide column is placed inside the coil pipe rack, and the light shield is installed outside the coil pipe rack.

[0004] Preferably, part of the cultivation pipeline is wound around the coil pipe rack.

[0005] Preferably, the cultivation pipeline is made of a breathable material.

[0006] Preferably, the cultivation pipeline is a Teflon tube.

[0007] Preferably, a plurality of magnets are arranged on the chassis, and a plurality of magnets are arranged at the bottom of the light shield, and the positions of the magnets on the chassis and the light shield correspond to each other.

[0008] Preferably, a plane mirror is arranged at one end of the light guide column, and the reflecting surface of the plane mirror faces the inside of the light guide column and is opposite to the lighting lamp.

[0009] Preferably, the inside of the light guide column is a hollow chamber, and the light guide column is made of a light-transmitting material.

[0010] Preferably, a guide groove is opened at the bottom of the light shield.

[0011] Preferably, a light intensity sensor is arranged on the device to detect the light intensity inside the lighting system.

[0012] The present utility model can achieve real-time detection during the algae cultivation process. By selecting a breathable cultivation pipeline, it can meet the gas exchange during the growth of algae. By winding the cultivation pipeline around a coil rack and then covering it with a light-shielding cover on the outside to form a closed space, the light intensity inside the space can be adjusted to meet the lighting requirements for different algae cultivation. Description of the Drawings

[0013] The above and / or additional aspects and advantages of the present utility model will become obvious and easy to understand from the description of the embodiments in conjunction with the following drawings, where:

[0014] Figure 1 is a structural diagram of the lighting system according to an embodiment of the present utility model;

[0015] Figure 2 is a structural diagram of the lighting system according to an embodiment of the present utility model;

[0016] Figure 3 is a structural diagram of a light guide column according to an embodiment of the present utility model;

[0017] Figure 4 is a sectional view according to an embodiment of the present utility model;

[0018] Figure 5 is a bottom plan view of the light-shielding cover according to an embodiment of the present utility model. Detailed Embodiments

[0019] The embodiments of the present utility model will be described in detail below. The examples of the embodiments are shown in the drawings, where the same or similar reference numerals represent the same or similar elements or elements with the same or similar functions throughout. The embodiments described below with reference to the drawings are exemplary and are only used to explain the present utility model and should not be construed as a limitation of the present utility model.

[0020] In the description of the present utility model, it should be understood that the orientation or positional relationship indicated by terms such as "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation to the present utility model. In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of the said features. In the description of the present utility model, "a plurality of" means two or more unless otherwise specifically defined.

[0021] In the description of the present utility model, it should be noted that unless otherwise clearly specified and limited, the terms "mounted", "connected" and "coupled" shall be understood in a broad sense. For example, it may be a fixed connection, a detachable connection or an integral connection; it may be a mechanical connection, an electrical connection or a connection capable of mutual communication; it may be directly connected or indirectly connected through an intermediate medium, and it may be the communication inside two elements or the interaction relationship between two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0022] In the description of the present utility model, unless otherwise clearly specified and limited, the first feature being "on" or "under" the second feature may include the direct contact between the first and second features, or may include the situation where the first and second features are not in direct contact but in contact through other features therebetween. Moreover, the first feature being "above", "over" and "on top of" the second feature includes that the first feature is directly above and obliquely above the second feature, or merely means that the horizontal height of the first feature is higher than that of the second feature. The first feature being "under", "beneath" and "underneath" the second feature includes that the first feature is directly below and obliquely below the second feature, or merely means that the horizontal height of the first feature is lower than that of the second feature.

[0023] The following disclosure provides many different embodiments or examples for implementing different structures of the present utility model. To simplify the disclosure of the present utility model, the components and settings of specific examples are described below. Of course, they are merely examples and are not intended to limit the present utility model. In addition, the present utility model may repeat reference numerals and / or reference letters in different examples. Such repetition is for the purpose of simplification and clarity and does not itself indicate the relationship between the various embodiments and / or settings discussed. In addition, the present utility model provides examples of various specific processes and materials, but those of ordinary skill in the art can be aware of the application of other processes and / or the use of other materials.

[0024] Referring to Figures 1-5 , a microfluidic device for light cultivation according to an embodiment of the present utility model includes a sample injection system, a microfluidic chip, a culture pipeline, a droplet identification system, a droplet detection system, a control system, and a light system. The sample injection system is used to inject an aqueous phase and an oil phase into the light cultivation device. The light system includes a chassis 1, a coil rack 2, a light lamp 3, a light guide column 7, and a light shielding cover 4. The coil rack 2 is fixedly connected to the chassis 1, the light lamp 3 is fixedly installed at the other end of the coil rack 2, the light guide column 7 is placed inside the coil rack 2, and the light shielding cover 4 is installed outside the coil rack 2.

[0025] Specifically, a microfluidic device for light cultivation according to an embodiment of the present utility model includes a sample injection system, a microfluidic chip, a culture pipeline, a droplet identification system, a droplet detection system, a control system, and a light system.

[0026] The sample injection system is used to inject an aqueous phase and an oil phase into the light cultivation device. The aqueous phase and the oil phase are immiscible liquids. The aqueous phase may include a culture medium and a certain number of algal cells.

[0027] The sample injection system is connected to the microfluidic chip, and microdroplets alternating between the aqueous phase and the oil phase are formed after passing through the microfluidic chip.

[0028] In this embodiment, the microdroplets enter the culture pipeline through the microfluidic chip for cultivation. The culture pipeline is located inside the light system, and the light intensity inside the light system is adjusted according to the growth conditions of different algal cells.

[0029] During the cultivation process, the droplets move in a reciprocating manner inside the pipeline. After the droplet identification system identifies the droplets, the droplets are numbered, and the droplet detection system performs spectral detection on the microdroplets passing through the detection point to judge the growth situation of the algae inside the culture pipeline.

[0030] Specifically, a part of the culture pipeline is wound around the coil rack 2. During the cultivation process, the microdroplets containing algal cells are located inside the wound pipeline, which is convenient for adjusting the light environment.

[0031] The culture pipeline usually selects a breathable material to facilitate the gas exchange between the algae in the culture pipeline and the external environment, and is preferably a transparent or light-transmitting Teflon tube.

[0032] A number of magnets 5 are arranged on the chassis 1, and a number of magnets 5 are arranged at the bottom of the light-shielding cover 4. The positions of the magnets 5 on the chassis 1 and the light-shielding cover 4 correspond to each other. With such a setting, the chassis 1 and the light-shielding cover 4 are tightly adsorbed under the action of the magnets 5, playing a supporting role for the internal coil rack 2 and ensuring the stability of the device.

[0033] The inside of the light guide column 7 is a hollow chamber. One end of the light guide column 7 uses a plane mirror 6 with good reflection effect, and its reflection plane faces the lighting lamp 3. The light guide column 7 is made of a light-transmitting material, which can be plastic, glass, resin, etc., and is not limited thereto.

[0034] Specifically, when the lighting lamp 3 is turned on, the light passes through the light guide column 7 to create a lighting environment. One end of the light guide column 7 has a cover opposite to the lighting lamp 3, and the inner side of the cover uses a plane mirror 6. Due to its good reflection effect, the lighting can be enhanced. The light guide column 7 can use a material with a matte texture. Compared with a purely transparent material, the uneven matte surface enhances the diffuse reflection of light, making the lighting effect in the device more uniform.

[0035] A guide groove 8 is opened at the bottom of the light-shielding cover 4 to facilitate the culture pipeline to pass through the guide groove 8 and be placed inside the light-shielding cover 4.

[0036] The device is also provided with a light intensity sensor 9 for detecting the light intensity inside the lighting system, so that the light intensity can be adjusted according to the needs, thereby regulating the growth process of the algae.

[0037] A microfluidic device capable of light illumination culture provided by the embodiment of the present invention, in the microfluidic system, by adjusting the light intensity, meets the light intensity requirements for the growth of algae; the microfluidic system has the characteristics of miniaturization, reduces the consumption of reagents, and can divide and fuse micro-droplets by using the precise control of the injection system and the microfluidic chip, realizing the function of automatic passage, greatly reducing the usage amount of reagents.

[0038] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and purposes of the present invention. The scope of the present invention is defined by the claims and their equivalents.

Claims

1. A microfluidic device capable of light-induced culture, comprising a sample introduction system, a microfluidic chip, a culture circuit, a droplet recognition system, a droplet detection system, a control system and a light-induced system, wherein the sample introduction system is used to introduce water phase and oil phase into the device to form microliter-scale water-in-oil droplets, characterized in that: The lighting system includes a chassis, a coil rack, a lighting lamp, a light guide column, and a light shield. The coil rack is fixedly connected to the chassis, the lighting lamp is fixed at the other end of the coil rack, the light guide column is placed inside the coil rack, and the light shield is installed on the outside of the coil rack.

2. A microfluidic device capable of light-induced culture according to claim 1, characterized in that: The culture tubing portion is wound on a coil rack.

3. The microfluidic device capable of light-induced culture according to claim 1, characterized in that: The culture pipeline is made of gas permeable material.

4. The microfluidic device capable of light-induced culture according to claim 1, characterized in that: The culture pipeline is a Teflon tube.

5. The microfluidic device capable of light-induced culture according to claim 1, characterized in that: A plurality of magnets are arranged on the chassis, and a plurality of magnets are arranged at the bottom of the light shield, and the positions of the magnets on the chassis and the light shield correspond to each other.

6. The microfluidic device capable of light-induced culture according to claim 1, characterized in that: A plane mirror is arranged at one end of the light guide column, and a reflective surface of the plane mirror faces the inner side of the light guide column and is opposite to the illumination lamp.

7. The microfluidic device capable of light-induced culture according to claim 6, characterized in that: The interior of the light guide column is a hollow chamber, and the light guide column is made of light-transmitting material.

8. The microfluidic device capable of light-induced culture according to claim 1, characterized in that: A guide groove is arranged at the bottom of the light shield.

9. The microfluidic device capable of light-induced culture according to claim 1, characterized in that: The device is provided with a light intensity sensor for detecting the light intensity inside the lighting system.

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