Separation device of glucuronidase

Abstract

The utility model relates to a glucuronidase separating device which comprises a centrifugal machine body and a base installed at the bottom of the centrifugal machine body in a sliding mode. The two sides of the base are each provided with an adsorption piece used for firmly placing the base. A buffering piece used for reducing vibration transmission of the centrifugal machine body is installed in the base. The buffer part comprises four push rods hinged to the bottom of the centrifugal machine body, the centrifugal machine body is started to work, generated vibration is transmitted to the four push rods, the four push rods push the moving rod to move, the piston block at one end of the moving rod reciprocates in the sealing frame, the sealing frame is filled with damping oil, and in the moving process of the piston block, the piston block is driven by the damping oil. Damping oil in the sealing frame circulates through the circulation holes in the surface of the piston block, so that large damping force is generated, resistance borne by the piston block in the vibration process can enable the vibration amplitude of the piston block to be gradually reduced, and therefore energy absorption buffering is conducted on vibration generated during work of the centrifugal machine body.

Description

Technical Field

[0001] The utility model relates to the technical field of glucuronidase processing equipment, and specifically relates to a separation device for glucuronidase. Background Art

[0002] Bilirubin in pig bile mainly exists in the form of a conjugate with glucuronic acid. The traditional production process uses chemical methods such as high temperature and strong alkali to remove the glucuronic acid group, resulting in easy oxidation of bilirubin, low yield, and serious environmental pollution. By screening glucuronidase that effectively hydrolyzes the conjugate to free bilirubin and developing its high-efficiency expression system, and optimizing the high-density fermentation process to improve the expression efficiency of glucuronidase, it is necessary to break the genetically engineered bacteria to release it and then separate the glucuronidase. A centrifuge is commonly used in biochemistry and molecular biology experiments to separate solid and liquid components in cells, tissues, or reaction solutions, and is one of the indispensable devices in the extraction and purification process. In the prior art, the bottom of the centrifuge base lacks a damper for buffering, and the buffering performance is poor. Since the centrifuge generates vibrations on the base during operation, after long-term use, the centrifuge body is prone to looseness, and due to the influence of vibrations, the placement of the separation device is not firm enough.

[0003] For example, a crystallized glucose saccharification liquid oil film separation device disclosed in the authorized patent document with the application number CN202221290717.0 includes a box body, an oil outlet pipe, a hose, a funnel, and a collection component; the collection component includes a discharge pipeline, a connecting rod, a support rod, a rotating edge, and a driving component. The discharge pipeline is communicated with the box body, the connecting rod is connected to the box body through the driving component, the support rod is fixedly connected to the connecting rod, the rotating edge is fixedly connected to the support rod, and the driving component is arranged on the box body and connected to the connecting rod. In the utility model, when the support rod drives the rotating edge to rotate rapidly, the saccharification liquid is driven to flow out of the discharge pipeline rapidly, thereby improving the flow rate of the saccharification liquid flowing out of the box body, and further improving the processing efficiency.

[0004] The above-mentioned patent has the problem that the bottom of the centrifuge base lacks a damper for buffering, and the buffering performance is poor. Since the centrifuge generates vibrations on the base during operation, after long-term use, the centrifuge body is prone to looseness. Therefore, we need to provide a separation device for glucuronidase. Summary of the Utility Model

[0005] The purpose of the present utility model is to provide a separation device for glucuronidase. By first attaching the two suction cups at the bottom of the two plate bodies to the workbench and pulling the pull plate to drive the two pull rods to move, the pull rods will drive the sealing pistons at their bottoms to move inside the tubes. Since the sealing pistons are rubber pistons and fit tightly with the inner walls of the tubes, and at the same time the suction cups are rubber discs and fit tightly with the top of the workbench, a negative pressure chamber is formed at the bottom of the sealing pistons, similar to the inside of a syringe, so that the four suction cups are firmly adsorbed on the workbench. And rotate the abutting bolt so that the bottom of the abutting bolt fits with the top of the plate body to prevent the pull plate from descending, thereby firmly adsorbing the base on the workbench, and at the same time reducing the generation of noise. Put the glucuronidase sample into the centrifuge body for separation treatment, start the centrifuge body to work, and the generated vibration is transmitted to the four push rods. The four push rods push the moving rod to move, so that the piston block at one end of the moving rod reciprocates in the sealing frame. There is damping oil filled in the sealing frame. During the movement of the piston block, the damping oil in the sealing frame circulates through the circulation holes on the surface of the piston block, thereby generating a large damping force. Among them, the resistance received by the piston block during vibration will gradually reduce the vibration amplitude of the piston block, thereby absorbing energy and buffering the vibration generated by the work of the centrifuge body, and solving the problems raised in the above-mentioned background technology.

[0006] To achieve the above object, the present utility model provides the following technical solutions: A separation device for glucuronidase, comprising:

[0007] A centrifuge body, and a base slidably installed at the bottom of the centrifuge body;

[0008] Suction accessories for firmly placing the base are installed on both sides of the base;

[0009] A buffer is installed inside the base for reducing the vibration transmission of the centrifuge body;

[0010] The buffer includes four push rods hinged to the bottom of the centrifuge body. One end of each of the four push rods is hinged with a moving rod. A sealing frame is slidably installed on one side of the moving rod. The bottoms of the four sealing frames are fixedly installed on the inner wall of the base. One end of the moving rod is fixedly installed with a piston block, and circulation holes are formed at the four corners of the surface of the piston block.

[0011] Preferably, support sliders are fixedly installed at the bottoms of the four moving rods, and the bottoms of the four support sliders are all in contact with the bottom of the inner wall of the base.

[0012] Preferably, cylinders are fixedly installed at the four corners of the bottom of the centrifuge body. The bottoms of the four cylinders are slidably installed with abutting rods, and rubber layer blocks are installed at the tops of the four cylinders.

[0013] Preferably, the adsorbing member includes a plate body fixedly installed on the surface of the base. Two tubes are embedded in each of the two plate bodies. Suction cups are connected to the bottoms of the two tubes. Sealing pistons are installed inside the two tubes. Tie rods are fixedly installed at the tops of the two sealing pistons, and a pull plate is fixedly installed at the upper ends of the two tie rods.

[0014] Preferably, the four suction cups are all made of rubber.

[0015] Preferably, a counterbore bolt is installed inside the pull plate in a threaded manner, and the bottom of the counterbore bolt is in contact with the top of the plate body.

[0016] Preferably, the sealing piston is a rubber piston, and the surface of the sealing piston is in close contact with the inner wall of the tube.

[0017] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0018] When the centrifuge body of the present utility model is started to work, the generated vibration is transmitted to the four push rods. The four push rods push the moving rod to move, so that the piston block at one end of the moving rod reciprocates in the sealing frame. The damping oil is filled in the sealing frame. During the movement of the piston block, the damping oil in the sealing frame flows through the flow holes on the surface of the piston block, thereby generating a large damping force. Among them, the resistance received by the piston block during vibration will gradually reduce the vibration amplitude of the piston block, thereby absorbing energy and buffering the vibration generated during the operation of the centrifuge body. Description of the Drawings

[0019] Figure 1 is a three-dimensional structural schematic diagram of the present utility model;

[0020] Figure 2 is a three-dimensional sectional view of the tube of the present utility model;

[0021] Figure 3 is a three-dimensional sectional view of the base of the present utility model;

[0022] Figure 4 is a three-dimensional view of the buffer member of the present utility model;

[0023] Figure 5 is a three-dimensional sectional view of the cylinder of the present utility model.

[0024] In the figure: 1. Centrifuge body; 2. Base; 3. Adsorbing member; 31. Plate body; 32. Tube; 33. Suction cup; 34. Sealing piston; 35. Tie rod; 36. Pull plate; 4. Buffer member; 41. Push rod; 42. Moving rod; 43. Sealing frame; 44. Piston block; 45. Flow hole; 5. Support sliding seat; 6. Cylinder; 7. Counterbore rod; 8. Rubber layer block; 9. Counterbore bolt. Detailed implementation mode

[0025] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0026] Please refer to Figures 1-5 , the present invention provides a technical solution: a separation device for glucuronidase, including:

[0027] A centrifuge body 1 and a base 2 slidably installed at the bottom of the centrifuge body 1;

[0028] Both sides of the base 2 are provided with suction attachments 3 for firmly placing the base 2;

[0029] A buffer member 4 for reducing the vibration transmission of the centrifuge body 1 is installed inside the base 2;

[0030] The buffer member 4 includes four push rods 41 hinged to the bottom of the centrifuge body 1. One end of each of the four push rods 41 is hinged with a moving rod 42. A sealing frame 43 is slidably installed on one side of the moving rod 42. The bottoms of the four sealing frames 43 are fixedly installed on the inner wall of the base 2. One end of the moving rod 42 is fixedly installed with a piston block 44. Flow holes 45 are formed at the four corners of the surface of the piston block 44.

[0031] Support sliding seats 5 are fixedly installed at the bottoms of the four moving rods 42, and the bottoms of the four support sliding seats 5 are all in contact with the bottom of the inner wall of the base 2;

[0032] In this embodiment, first, the two suction cups 33 at the bottom of the two plate bodies 31 are attached to the workbench, and the pull plate 36 is pulled to drive the two pull rods 35 to move. The pull rods 35 will drive the sealing pistons 34 at their bottoms to move within the tube bodies 32. Since the sealing pistons 34 are rubber pistons and fit tightly with the inner walls of the tube bodies 32, and at the same time the suction cups 33 are rubber discs and fit tightly with the top of the workbench, a negative pressure chamber is formed at the bottom of the sealing pistons 34, similar to the inside of a syringe, so that the four suction cups 33 are firmly adsorbed on the workbench. Then, the abutting bolt 9 is rotated so that the bottom of the abutting bolt 9 fits with the top of the plate body 31 to prevent the pull plate 36 from descending, thereby firmly adsorbing the base 2 on the workbench and reducing the generation of noise at the same time. The glucuronidase sample is placed into the centrifuge body 1 for separation processing. When the centrifuge body 1 is started to work, the generated vibration is transmitted to the four push rods 41, and the four push rods 41 push the moving rod 42 to move, so that the piston block 44 at one end of the moving rod 42 reciprocates within the sealing frame 43. The sealing frame 43 is filled with damping oil. During the movement of the piston block 44, the damping oil in the sealing frame 43 flows through the circulation holes 45 on the surface of the piston block 44, thereby generating a large damping force. Among them, the resistance that the piston block 44 receives during vibration will gradually reduce the vibration amplitude of the piston block 44, thereby absorbing and buffering the vibration generated by the operation of the centrifuge body 1. As the moving rod 42 moves, the supporting sliding seat 5 follows the movement to serve the purpose of assisting in supporting the moving rod 42;

[0033] The damping size can be adjusted by adjusting the design of the through holes of the circulation holes 45 on the surface of the piston block 44 (such as aperture size, shape, quantity, etc.) and the viscosity of the damping oil.

[0034] Four cylinders 6 are fixedly installed at the four corners of the bottom of the centrifuge body 1. The bottoms of the four cylinders 6 are all slidably installed with abutting rods 7, and rubber layer blocks 8 are installed at the tops of the four cylinders 6;

[0035] Specifically, as the centrifuge body 1 moves, it will drive the cylinders 6 to move on the abutting rods 7, and the abutting rods 7 will squeeze the rubber layer blocks 8, and the rubber layer blocks 8 will deform to absorb the impact force to serve the purpose of absorbing and buffering energy.

[0036] The suction attachment 3 includes a plate body 31 fixedly installed on the surface of the base 2. Two tube bodies 32 are embedded inside the two plate bodies 31. The bottoms of the two tube bodies 32 are both communicated with suction cups 33. Sealing pistons 34 are installed inside the two tube bodies 32. Pull rods 35 are fixedly installed at the tops of the two sealing pistons 34, and a pull plate 36 is fixedly installed at the upper ends of the two pull rods 35;

[0037] Among them, the two adsorption plates 33 at the bottom of the two plate bodies 31 are attached to the workbench, and the pull plate 36 is pulled to drive the two pull rods 35 to move. The pull rod 35 will drive the sealing piston 34 at its bottom to move in the tube body 32. Since the sealing piston 34 is a rubber piston and fits tightly with the inner wall of the tube body 32, and the adsorption plate 33 is a rubber plate and fits tightly with the top of the workbench, a negative pressure chamber is formed at the bottom of the sealing piston 34, similar to the inside of a syringe, so that the four adsorption plates 33 are firmly adsorbed on the workbench.

[0038] The four adsorption discs 33 are all configured as rubber discs;

[0039] The working principle of the rubber adsorption disc 33 is based on the elasticity of rubber and the principle of vacuum negative pressure. When the rubber adsorption disc 33 is in close contact with the surface of an object, the air between the adsorption disc 33 and the object is expelled by squeezing, forming a relatively closed space. After the air in this closed space is expelled, a low-pressure area is formed, that is, a negative pressure state. Due to the existence of external atmospheric pressure, this low-pressure area will produce an inward adsorption force on the object.

[0040] The inner thread of the pull plate 36 is provided with a resisting bolt 9, and the bottom of the resisting bolt 9 is fitted with the top of the plate body 31;

[0041] Specifically, the interference bolt 9 is rotated to make the bottom of the interference bolt 9 fit with the top of the plate body 31 to prevent the pull plate 36 from falling, thereby firmly adsorbing the base 2 on the workbench and reducing the generation of noise.

[0042] The sealing piston 34 is configured as a rubber piston, and the surface of the sealing piston 34 is closely fitted to the inner wall of the tube body 32;

[0043] Furthermore, the sealing piston 34 is a rubber piston and fits tightly against the inner wall of the tube body 32. Meanwhile, the adsorption disk 33 is a rubber disk and fits tightly against the top of the workbench, forming a negative pressure chamber at the bottom of the sealing piston 34, similar to the inside of a syringe, so that the four adsorption disks 33 are firmly adsorbed on the workbench.

[0044] The device first attaches the two suction cups 33 at the bottom of the two plate bodies 31 to the workbench, and pulls the pull plate 36 to drive the two pull rods 35 to move. The pull rods 35 will drive the sealing pistons 34 at their bottoms to move within the tube bodies 32. Since the sealing pistons 34 are rubber pistons and fit tightly with the inner walls of the tube bodies 32, and at the same time the suction cups 33 are rubber discs and fit tightly with the top of the workbench, a negative pressure chamber is formed at the bottom of the sealing pistons 34, similar to the inside of a syringe, so that the four suction cups 33 are firmly adsorbed on the workbench. Then, turn the abutting bolt 9 so that the bottom of the abutting bolt 9 fits with the top of the plate body 31 to prevent the pull plate 36 from descending, thereby firmly adsorbing the base 2 on the workbench and reducing the generation of noise at the same time. Put the glucuronidase sample into the centrifuge body 1 for separation processing. Start the centrifuge body 1 to work. The generated vibration is transmitted to the four push rods 41, and the four push rods 41 push the moving rod 42 to move, so that the piston block 44 at one end of the moving rod 42 reciprocates within the sealing frame 43. The damping oil is filled in the sealing frame 43. During the movement of the piston block 44, the damping oil in the sealing frame 43 flows through the flow holes 45 on the surface of the piston block 44, thereby generating a large damping force. Among them, the resistance received by the piston block 44 during vibration will gradually reduce the vibration amplitude of the piston block 44, thereby absorbing energy and buffering the vibration generated by the operation of the centrifuge body 1.

[0045] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principle and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A glucuronidase separation device, characterized in that: include: A centrifuge body (1), and a base (2) slidably mounted on the bottom of the centrifuge body (1); Adsorption components (3) for firmly placing the base (2) are installed on both sides of the base (2); A buffer (4) is installed inside the base (2) for reducing vibration transmission of the centrifuge body (1); The buffer member (4) comprises four push rods (41) hinged on the bottom of the centrifuge body (1), one end of each of the four push rods (41) is hinged with a moving rod (42), a sealing frame (43) is slidably mounted on one side of the moving rod (42), the bottoms of the four sealing frames (43) are fixedly mounted on the inner wall of the base (2), one end of each of the moving rods (42) is fixedly mounted with a piston block (44), and four corners of the surface of the piston block (44) are provided with flow holes (45).

2. A glucuronidase separation device according to claim 1, characterized in that: The bottoms of the four moving rods (42) are all fixedly mounted with support slides (5), and the bottoms of the four support slides (5) are all in contact with the bottom of the inner wall of the base (2).

3. A glucuronidase separation device according to claim 1, characterized in that: Cylinders (6) are fixedly mounted at the four corners of the bottom of the centrifuge body (1), abutment rods (7) are slidably mounted at the bottoms of the four cylinders (6), and adhesive blocks (8) are mounted at the tops of the four cylinders (6).

4. The separation device for glucuronidase according to claim 1, characterized in that: The adsorption component (3) comprises a plate body (31) fixedly mounted on the surface of the base (2), two tube bodies (32) are embedded in the two plate bodies (31), the bottoms of the two tube bodies (32) are connected to adsorption plates (33), the interiors of the two tube bodies (32) are installed with sealing pistons (34), the tops of the two sealing pistons (34) are fixedly mounted with pull rods (35), and the upper ends of the two pull rods (35) are fixedly mounted with pull plates (36).

5. A glucuronidase separation device according to claim 4, characterized in that: The four adsorption discs (33) are all configured as rubber discs.

6. A glucuronidase separation device according to claim 4, characterized in that: The inner thread of the pull plate (36) is provided with a resisting bolt (9), and the bottom of the resisting bolt (9) is in contact with the top of the plate body (31).

7. A glucuronidase separation device according to claim 4, characterized in that: The sealing piston (34) is configured as a rubber piston, and the surface of the sealing piston (34) is tightly fitted to the inner wall of the tube body (32).

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