Genomic DNA extraction device

Abstract

The utility model provides a genome DNA (deoxyribonucleic acid) extraction device, and mainly relates to the technical field of reagent suction equipment. The genome DNA extraction device comprises a base, a limiting plate is fixedly installed at the top of the base, a first through hole is formed in the top face of the limiting plate, rubber balls are fixedly installed at the movable ends of spring telescopic rods respectively, a centrifugal test tube is movably inserted into the first through hole, a suction pipe is placed in the centrifugal test tube, and a suction pipe is arranged in the suction pipe. The lower end of the centrifugal test tube is inserted into the groove and is in contact fit with the groove, a positioning assembly is arranged on the top surface of the limiting plate, and the positioning assembly can limit the suction tube. The device has the beneficial effects that when the device is used, a centrifugal test tube is clamped and positioned through the mutual matching of the spring telescopic rods in the limiting plate, and the downward moving range of the suction tube is determined through the pointer flush with the bottom end of the suction tube, so that the instability of judging the layering position while manually taking the suction tube to suck liquid is improved; the separation purity of the extracting solution is improved.

Description

Technical Field

[0001] The utility model mainly relates to the technical field of reagent suction equipment, and specifically relates to a genomic DNA extraction device. Background Technique

[0002] Preparing genomic DNA is an important step in studying gene structure and function, and usually requires the obtained fragment length to be not less than 100 - 200 kb. During the DNA extraction process, DNA breakage and degradation should be avoided as much as possible to ensure the integrity of DNA. Extracting DNA through chloroform is one of the commonly used gene extraction methods in genomic research. The stability of the protein colloid is destroyed by an organic solution to precipitate and separate the protein, and then DNA is precipitated by absolute ethanol. During this process, the extraction solution mixed with protease is kept warm, and then the reaction solution added with saturated phenol solution is centrifuged. The upper viscous aqueous phase in the centrifuge tube is sucked out through a pipette, and then the DNA in the form of a cloud-like floc is separated by absolute ethanol.

[0003] In the prior art, during the extraction of DNA in a centrifuge tube, it is necessary to manually suck the layered reaction solution multiple times. The operator needs to judge the stratification position of the extraction solution to stop sucking. And during the operation, due to the easy shaking of the pipette held by hand, it will cause a certain degree of precipitation disturbance, thus increasing the difficulty of judging the stratification position of the reaction solution, affecting the purity of the remaining extraction solution in the centrifuge tube, and multiple extractions are required to ensure the DNA purity, resulting in low extraction efficiency. Content of the Utility Model

[0004] To solve the deficiencies of the prior art, the utility model provides a genomic DNA extraction device, which is realized through the following technical solutions:

[0005] A genomic DNA extraction device includes a base. A limiting plate is fixedly installed on the top of the base. A first through hole is formed on the top surface of the limiting plate. A plurality of spring telescopic rods distributed in an annular array are fixedly installed in the first through hole. The movable ends of the spring telescopic rods are respectively fixedly installed with rubber balls. A groove coaxial with the first through hole is formed in the center of the top surface of the base. A centrifuge tube is movably inserted into the first through hole. A pipette is placed in the centrifuge tube. The lower end of the centrifuge tube is inserted into the groove and is in contact and cooperation with it. The outer circumference of the centrifuge tube abuts against all the rubber balls. A positioning component is arranged on the top surface of the limiting plate, and the positioning component can limit the pipette.

[0006] Furthermore, support legs are respectively fixedly installed at the four corners of the bottom surface of the limiting plate, and the lower ends of the support legs are respectively fixedly connected with the top surface of the base.

[0007] Further, the positioning component includes a rotating shaft. One side of the top surface of the limiting plate is installed with a rotating shaft through a bearing. The upper end of the rotating shaft is fixedly installed with a first connecting plate. A second through hole is opened on the top surface of the first connecting plate, and the second through hole is coaxial with the first through hole. The straw is inserted into the second through hole, and a lifting component capable of adjusting the position of the straw is fixedly installed on the first connecting plate.

[0008] Further, the lifting component includes a support block. The support block is fixedly installed on the front surface of the first connecting plate. A square groove is opened on the top surface of the support block, and a square rod is slidably fitted in the square groove. The upper end of the square rod is fixedly installed with a second connecting plate. A third through hole coaxial with the second through hole is opened on the top surface of the second connecting plate. The straw is inserted into the third through hole, the second through hole from top to bottom and extends into the centrifuge tube. A locking component capable of fixing the square rod is arranged in the support block. A horizontally arranged pointer is fixedly installed on the lower part of the outer periphery of the square rod, and the pointer is flush with the lower end of the straw.

[0009] Further, the locking component includes a locking nut. A threaded hole communicating with the square groove is opened on the outer side of the support block, and the locking nut is threadedly fitted in the threaded hole. The inner end of the locking nut abuts against one side of the square rod.

[0010] Further, a limiting rod is fixedly installed on the top surface of the base. A blind hole matched with the limiting rod is opened at the lower end of the square rod, and the limiting rod can be inserted into the blind hole.

[0011] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0012] When the device is in use, the centrifuge tube is clamped and positioned through the mutual cooperation between the spring telescopic rods inside the limiting plate. After the extraction liquid in the centrifuge tube is layered, the straw is limited by the positioning component, and the downward movement range of the straw is determined through the pointer flush with the bottom end of the straw, so as to improve the instability of manually holding the straw to suck the liquid while judging the layering position, reduce the operation difficulty of DNA extraction, and improve the separation purity of the extraction liquid. Description of the Drawings

[0013] Figure 1 is a schematic structural diagram of the present utility model;

[0014] Figure 2 is a front view of the present utility model;

[0015] Figure 3 is a schematic cross-sectional structure diagram of the limiting plate of the present utility model.

[0016] Reference numerals shown in the drawings: 1, base; 2, limit plate; 3, first through hole; 4, spring telescopic rod; 5, rubber ball; 6, groove; 7, centrifuge tube; 8, straw; 9, support leg; 10, rotating shaft; 11, first connecting plate; 12, second through hole; 13, support block; 14, square groove; 15, square rod; 16, second connecting plate; 17, third through hole; 18, pointer; 19, locking nut; 20, screw hole; 21, limit rod; 22, blind hole. Detailed implementation manners

[0017] In combination with the drawings and specific embodiments, the present invention will be further described. It should be understood that these embodiments are only used to illustrate the present invention and not to limit the scope of the present invention. In addition, it should be understood that after reading the content taught by the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by this application.

[0018] Embodiment: A genomic DNA extraction device

[0019] As Figures 1-3 shown, a genomic DNA extraction device, its specific structure includes:

[0020] A base 1, a limit plate 2 is fixedly installed on the top of the base 1, a first through hole 3 is opened on the top surface of the limit plate 2, a plurality of spring telescopic rods 4 distributed in a circular array are fixedly installed in the first through hole 3, the movable ends of the spring telescopic rods 4 all face the central axis of the first through hole 3, rubber balls 5 are respectively fixedly installed at the movable ends of the spring telescopic rods 4, a groove 6 coaxial with the first through hole 3 is opened at the center of the top surface of the base 1, a centrifuge tube 7 is movably inserted into the first through hole 3, a straw 8 is placed in the centrifuge tube 7, the lower end of the centrifuge tube 7 is inserted into the groove 6 and is in contact and cooperation with it, the outer circumference of the centrifuge tube 7 abuts against all the rubber balls 5, and a positioning component is provided on the top surface of the limit plate 2, and the positioning component can limit the straw 8.

[0021] Support legs 9 are respectively fixedly installed at the four corners of the bottom surface of the limit plate 2, and the lower ends of the support legs 9 are respectively fixedly connected to the top surface of the base 1. The support of the limit plate 2 is realized through the support legs 9, so that the limit plate 2 can support the middle part of the centrifuge tube 7, and the stability of the device when clamping the centrifuge tube 7 is improved.

[0022] The positioning component includes a rotating shaft 10. One side of the top surface of the limiting plate 2 is installed with the rotating shaft 10 through a bearing. The upper end of the rotating shaft 10 is fixedly installed with a first connecting plate 11. A second through hole 12 is opened on the top surface of the first connecting plate 11. The second through hole 12 is coaxial with the first through hole 3. The straw 8 is inserted into the second through hole 12. An elevating component capable of adjusting the position of the straw 8 is fixedly installed on the first connecting plate 11. When this structural design is in use, the rotating shaft 10 can drive the connected first connecting plate 11 to rotate, so that the second through hole 12 on the top surface of the first connecting plate 11 is located above the centrifuge tube 7, thereby realizing the positioning of the straw 8. After the straw 8 is inserted into the second through hole 12, the height of the straw 8 can be adjusted through the elevating component, and then the extraction solution in the centrifuge tube 7 can be sucked through the straw 8.

[0023] The elevating component includes a support block 13. The support block 13 is fixedly installed on the front surface of the first connecting plate 11. A square groove 14 is opened on the top surface of the support block 13. A square rod 15 is slidably installed in the square groove 14 in a mating manner. The upper end of the square rod 15 is fixedly installed with a second connecting plate 16. A third through hole 17 coaxial with the second through hole 12 is opened on the top surface of the second connecting plate 16. The straw 8 is inserted into the third through hole 17, the second through hole 12 in sequence from top to bottom and extends into the centrifuge tube 7. A locking component capable of fixing the square rod 15 is arranged in the support block 13. A horizontally arranged pointer 18 is fixedly installed on the lower part of the outer circumference of the square rod 15. The pointer 18 is flush with the lower end of the straw 8. When this structural design is in use, when the straw 8 is inserted into the third through hole 17, the liquid storage sac at the top of the straw 8 contacts the top of the second connecting plate 16. At this time, the lower end of the straw 8 is flush with the pointer 18. By moving the position of the square rod 15, the square rod 15 can drive the straw 8 to move synchronously through the second connecting plate 16, thereby realizing the adjustment of the position of the straw 8. By moving the pointer 18 to the extraction solution stratification part, the suction depth of the straw 8 can be controlled, and the accuracy of extracting the centrifuged DNA mixed solution can be improved.

[0024] The locking component includes a locking nut 19. A threaded hole 20 communicating with the square groove 14 is opened on the outer side of the support block 13. The locking nut 19 is threadedly installed in the threaded hole 20. The inner end of the locking nut 19 abuts against one side of the square rod 15. When this structural design is in use, by rotating the locking nut 19, it can move along the axial direction of the threaded hole 20, so that the locking nut 19 can tightly press the square rod 15 to fix it, or the locking nut 19 can move outwards to loosen the square rod 15. The structure is simple and convenient to use.

[0025] A limiting rod 21 is fixedly installed on the top surface of the base 1. A blind hole 22 matching with the limiting rod 21 is formed at the lower end of the square rod 15, and the limiting rod 21 can be inserted into the blind hole 22. The positioning of the square rod 15 is realized through the limiting rod 21. During use, the first connecting plate 11 is rotated to a position above the centrifuge tube 8 by rotating the rotating shaft 10. At the same time, the second connecting plate 16 drives the square rod 15 to move above the limiting rod 21 through the support block 13. The square rod 15 is moved downward to insert the limiting rod 21 into the blind hole 22, and the positioning of the shift lever 15 can be realized, which can avoid the shaking of the first connecting plate 11 and the second connecting plate 16 during the use of the pipette 8, improve the stability of the pipette 8 during use, avoid stirring the liquid in the centrifuge tube 7 during the use of the centrifuge tube 8, and prevent the liquid in the centrifuge tube 7 from becoming turbid again.

[0026] Working principle:

[0027] When the device is in use, the support of the bottom of the centrifuge tube 7 and the limit of the middle part are realized through the mutual cooperation between the groove 6 on the top surface of the base 1 and the spring telescopic rod 4, improving the stability of the centrifuge tube 7 when placed. By moving the position of the square rod 15, the square rod 15 drives the pipette 8 to move synchronously through the second connecting plate 16, so as to realize the adjustment of the position of the pipette 8. By moving the pointer 18 to the extraction liquid stratification part, the suction depth of the pipette 8 can be controlled, which is convenient for the user to suck the DNA liquid in the centrifuge tube 7. After the work is completed, by rotating the rotating shaft 10, the rotating shaft 10 drives the first connecting plate 11 connected thereto to rotate, so that the first connecting plate 11 and the second connecting plate 16 move outside the base 1. At this time, the first connecting plate 11 and the second connecting plate 16 can be used as a storage rack to place the pipette 8, which is convenient for the user to use.

[0028] In the explanation of the present utility model, it should be noted that the term nouns indicating directions are only for the convenience of description and understanding, and do not uniquely limit the installation positions of specific technical features, and other installable ways that can be realized are not excluded.

[0029] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present utility model, rather than to limit them; although the present utility model has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements for some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the various embodiments of the present utility model.

Claims

1. A genomic DNA extraction device, comprising a base (1), a limit plate (2) fixedly mounted on the top of the base (1), a first through hole (3) being formed on the top surface of the limit plate (2), characterized in that: A plurality of spring telescopic rods (4) distributed in a circular array are fixedly mounted in the first through hole (3), and rubber balls (5) are fixedly mounted on the movable ends of the spring telescopic rods (4). A groove (6) coaxial with the first through hole (3) is provided at the center of the top surface of the base (1). A centrifugal test tube (7) is movably inserted in the first through hole (3), and a straw (8) is placed in the centrifugal test tube (7). The lower end of the centrifugal test tube (7) is inserted into the groove (6) and contacts and cooperates therewith, and the outer periphery of the centrifugal test tube (7) abuts against all the rubber balls (5). A positioning component is provided on the top surface of the limiting plate (2), and the positioning component can limit the straw (8).

2. A genomic DNA extraction device according to claim 1, characterized in that: Support legs (9) are fixedly mounted at the four corners of the bottom surface of the limiting plate (2), and the lower ends of the support legs (9) are fixedly connected to the top surface of the base (1).

3. A genomic DNA extraction device according to claim 1, characterized in that: The positioning assembly comprises a rotating shaft (10), a bearing on one side of the top surface of the limiting plate (2) is mounted with the rotating shaft (10), a first connecting plate (11) is fixedly mounted on the upper end of the rotating shaft (10), a second through hole (12) is formed on the top surface of the first connecting plate (11), the second through hole (12) is coaxial with the first through hole (3), the suction tube (8) is inserted into the second through hole (12), and a lifting assembly capable of adjusting the position of the suction tube (8) is fixedly mounted on the first connecting plate (11).

4. A genomic DNA extraction device according to claim 3, characterized in that: The lifting assembly comprises a support block (13), the support block (13) being fixedly mounted on the front of the first connecting plate (11), the top surface of the support block (13) being provided with a square groove (14), a square rod (15) being slidably mounted in the square groove (14), a second connecting plate (16) being fixedly mounted on the upper end of the square rod (15), a third through hole (17) being coaxial with the second through hole (12) being formed on the top surface of the second connecting plate (16), the suction tube (8) being inserted into the third through hole (17) and the second through hole (12) in sequence from top to bottom and extending into the centrifuge test tube (7), a locking assembly capable of fixing the square rod (15) being provided in the support block (13), a horizontally arranged pointer (18) being fixedly mounted on the lower part of the outer periphery of the square rod (15), the pointer (18) being flush with the lower end of the suction tube (8).

5. A genomic DNA extraction device according to claim 4, characterized in that: The locking assembly comprises a locking nut (19); a screw hole (20) communicating with the square groove (14) is formed on the outer side of the support block (13); the locking nut (19) is mounted on the inner thread of the screw hole (20); and the inner end of the locking nut (19) abuts against one side of the square rod (15).

6. A genomic DNA extraction device according to claim 4, characterized in that: A limiting rod (21) is fixedly mounted on the top surface of the base (1); a blind hole (22) matching the limiting rod (21) is formed at the lower end of the square rod (15); and the limiting rod (21) can be inserted into the blind hole (22).

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