Portable aboriginal microbiome ectopic trap

Abstract

The utility model discloses a portable aboriginal microbiome ectopic trap, relates to microbe culture and utilization technical field, the incubator, tray, culture box, temperature module, temperature sensor, humidity sensor and oxygen sensor, culture box and the tray are provided in the incubator, the tray is used for holding sample, the temperature module is used for holding the temperature module, the humidity sensor is used for holding the temperature module, the temperature sensor is used for holding the humidity sensor, and the oxygen sensor is used for holding the temperature module. The culture box is used for being placed on a sample on the tray, the temperature module is used for providing needed temperature for microorganisms, the temperature sensor is used for detecting the temperature of the environment where the culture box is located, and the humidity sensor is used for detecting the humidity of the environment where the culture box is located; the oxygen sensor is used for detecting the oxygen content of the environment where the culture box is located. The portable aboriginal microbiome ectopic trap disclosed by the utility model can be used for reducing the change degree of the environment and reducing the loss of microbial components caused by the change of the environment.

Description

Technical Field

[0001] The utility model relates to the technical field of microbial culture and utilization, in particular to a portable in-situ collector for indigenous microbiome. Background Art

[0002] Microorganisms are ubiquitous in human life. In the past, people's understanding of microorganisms only stayed on single-bacterium culture and qualitative research. With the breakthrough of high-throughput sequencing technology and the development of bioinformatics, the research of microbiomics has developed rapidly. Microorganisms and their surrounding environment together constitute a complex microbiome, which plays an irreplaceable role in supporting the processes and functions of ecosystems. The microbiome provides a powerful tool for regulating soil microorganisms, promoting soil health, improving crop production, and environmental remediation. Therefore, the role of the microbiome in environmental and agricultural sustainable development has been increasingly concerned.

[0003] In the past 50 years, humans have made rapid progress in understanding the diversity of environmental microorganisms and their roles in sustainable agriculture and environmental remediation. Recently, someone analyzed the experimental results of 81 experiments globally, showing that in ecosystems, the restoration of the indigenous microbiome in soil can increase the plant biomass by an average of 64%.

[0004] Indigenous microorganisms are a group of native microbiomes that live in harmony with the local nature, which is an aggregate of different beneficial microorganisms, including bacteria, fungi, yeasts, protozoa, etc., inhabiting on the surface of soil and all organisms. They are physiologically compatible and complementary to each other, with the potential of biodegradation, nitrogen fixation, improving soil fertility, dissolving phosphorus and potassium, promoting plant growth, etc., and can be effectively applied to many fields such as nature-based agricultural systems, production of biofertilizers, biocomposting of agricultural waste, bioleaching of heavy metals, biodegradation and bioremediation of organic pollutants such as petroleum and herbicides.

[0005] The application of indigenous microbiome in agriculture was first developed by Dr. Chou Han-kyu in Korea in the 1960s and has been scaled up in more than 30 countries, including Korea, Japan, China, Vietnam, Malaysia, Thailand, the Philippines, the United States, Congo, Tanzania, Nigeria, and Mongolia, etc.

[0006] How to effectively capture indigenous microorganisms is the first step in using indigenous microorganisms in agriculture and the environment. Currently, there are two methods for capturing indigenous microorganisms: in situ and ex situ. When collecting in situ, a container containing a carbohydrate-rich matrix (such as rice or potatoes) is generally buried in the surface soil of a pre-selected farmland or natural ecosystem. After 5-10 days, the microorganisms in the environment will penetrate into the matrix and form white colonies, and the collection work is completed. The above-mentioned collection is then mixed with sugar or molasses and fermented for about a week. The fermentation product is diluted with water or directly mixed with the soil and applied as an agricultural microbial agent or soil conditioner. In addition, a dedicated in situ culture box is also used for in situ capture of microorganisms.

[0007] Patent application 201710072815.4 discloses a method for producing microbial fertilizer, nutrient solution, feed, pesticide, and fermentation bed breeding with indigenous microbial probiotics. A fir wood box is 25 cm long, 20 cm wide, and 10 cm high. It is filled with slightly harder rice, which accounts for two-thirds of the wooden box, i.e., 1-1.5 kg. It is covered with rice paper to seal the box, tied with tape or rope, and buried in forest soil containing white humus leaves. A pit is dug with a depth of 35 cm, a length of 40 cm, and a width of 40 cm. The mushroom soil leaves with white mycelium from the forest are collected and laid flat on the bottom of the pit. The wooden box filled with rice is placed in the pit, and the surrounding Fill the rice into a steamed bun shape with white mycelium soil and cover it with plastic film to prevent it from being damaged by rain. After 5-7 days in spring and autumn and 3-4 days in summer, the beneficial microorganisms in the surrounding leaf mold will penetrate into the rice and form white and pink beneficial colonies. The rice will become a thin mud. After taking the rice back, immediately mix the rice with fermented brown sugar in a 1:1 ratio and put it into a ceramic jar, accounting for two-thirds of the jar. Cover it with rice paper to seal it and place it in a place with a temperature above 18 degrees. After 7-8 days, the rice will turn into a dark red solute liquid. After filtration, the indigenous microbial concentrate, that is, the original microbial species, is extracted, which contains photosynthetic bacteria, Bacillus, etc.

[0008] The in situ mode can capture the indigenous microbiome to the maximum extent while maintaining the specific environmental conditions of the region (temperature, humidity, soil, plants), and has high environmental compatibility, but it generally needs to be buried in the wild for about a week, and is easily disturbed by the outside world (such as heavy rain, animals, humans, etc.), with low repeatability, and it is often more difficult to collect samples from difficult-to-reach locations, increasing labor costs and time costs. The current ex situ collection method requires that soil or environmental samples be collected and brought into the laboratory. The culture conditions are easy to control, and after sample pretreatment, the repeatability of the collection results is improved, but the degree of change in environmental conditions is large, and the transportation process may cause the loss of certain microbial components, which is very different from the actual indigenous microbiome. Utility Model Content

[0009] The purpose of the present utility model is to provide a portable in-situ trap for indigenous microbiota, which reduces the degree of environmental change and the loss of microbial components caused by environmental change.

[0010] To achieve the above object, the present utility model provides the following solutions:

[0011] The present utility model provides a portable in-situ trap for indigenous microbiota, comprising: a heat preservation box, a tray, a culture box, a temperature module, a temperature sensor, a humidity sensor and an oxygen sensor. The culture box and the tray are arranged in the heat preservation box. The tray is used for holding samples, the culture box is used for placing on the samples on the tray. The temperature module is used to provide the required temperature for the microorganisms. The temperature sensor is used to detect the temperature of the environment where the culture box is located. The humidity sensor is used to detect the humidity of the environment where the culture box is located. The oxygen sensor is used to detect the oxygen content of the environment where the culture box is located.

[0012] Preferably, it further comprises a trapping box, which is located in the heat preservation box. A trapping cavity is formed in the trapping box, and the culture box is located in the trapping cavity.

[0013] Preferably, the temperature sensor, the humidity sensor and the oxygen sensor are all located in the trapping box.

[0014] Preferably, the tray is located in the trapping cavity, and there is a gap between the tray and the inner wall of the trapping box.

[0015] Preferably, there is a heat insulation structure between the bottom of the tray and the trapping box.

[0016] Preferably, the heat preservation box comprises a heat preservation box body and a heat preservation box cover, and the heat preservation box body and the heat preservation box cover are detachably connected.

[0017] Preferably, the trapping box is made of stainless steel. The trapping box comprises a trapping box body and a trapping box cover, and the trapping box body and the trapping box cover are detachably connected.

[0018] Preferably, the temperature module comprises a refrigeration module and a heating module.

[0019] Preferably, through holes are provided at the bottom of the culture box.

[0020] Preferably, it further comprises a battery.

[0021] The present utility model has achieved the following technical effects compared with the prior art:

[0022] The portable indigenous microbiome ectopic trap of the present utility model can perform ectopic collection of the indigenous microbiome. After the collection is completed, the sample can be placed in the same or similar temperature environment as during collection through the temperature sensor and the temperature module, the humidity can be measured through the humidity sensor to keep the sample in the same or similar humidity environment as during collection, and the oxygen content can be measured through the oxygen sensor to keep the sample in the same or similar oxygen content environment as during collection. Through the ectopic collection mode, the present utility model reduces the influence of environmental condition changes, maintains the integrity of the indigenous microbiome, has easy-to-control conditions, is convenient to carry, easy to use, and greatly saves the time and cost of field operations. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required for use in the embodiments. Obviously, the drawings in the following description are only some embodiments of the present utility model. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0024] Figure 1 Front view of the portable indigenous microbiome ectopic trap of the present utility model;

[0025] Figure 2 Internal top view of the portable indigenous microbiome ectopic trap of the present utility model;

[0026] Figure 3 Schematic diagram of the connection of each structure circuit of the portable indigenous microbiome ectopic trap of the present utility model;

[0027] Figure 4 Photo of the indigenous microbiome capture test (Application Example 1);

[0028] Figure 5 Photo of the indigenous microbiome capture test (Application Example 2);

[0029] In the figure: 1 - heat preservation box body, 2 - heat preservation box cover, 3 - constant temperature controller, 4 - monitor, 5 - heating switch, 6 - refrigeration switch, 7 - refrigeration module, 8 - heating module, 9 - temperature sensor, 10 - humidity sensor, 11 - oxygen sensor, 12 - battery. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0030] The following will clearly and completely describe the technical solutions in the embodiments of the present utility model with reference to the drawings in the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, rather than all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present utility model.

[0031] The object of the present utility model is to provide a portable in-situ collector for indigenous microbiota, which reduces the degree of environmental change and decreases the loss of microbial components caused by environmental change.

[0032] In order to make the above objects, features and advantages of the present utility model more obvious and understandable, the present utility model will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0033] As Figures 1 to 3 shown, this embodiment provides a portable in-situ collector for indigenous microbiota, including: a heat preservation box, a tray, a culture box, a temperature module, a temperature sensor 9, a humidity sensor 10 and an oxygen sensor 11. The culture box and the tray are arranged in the heat preservation box. The tray is used for holding samples, and the culture box is used for placing on the samples on the tray. The temperature module is used to provide the required temperature for the microorganisms. The temperature sensor 9 is used to detect the temperature of the environment where the culture box is located. The humidity sensor 10 is used to detect the humidity of the environment where the culture box is located. The oxygen sensor 11 is used to detect the oxygen content of the environment where the culture box is located. The portable in-situ collector for indigenous microbiota of this embodiment can collect indigenous microbiota in-situ. After the collection is completed, the samples can be placed in the same or similar temperature environment as that during collection through the temperature sensor 9 and the temperature module, the humidity can be measured through the humidity sensor 10 to make the samples in the same or similar humidity environment as that during collection, and the oxygen content can be measured through the oxygen sensor 11 to make the samples in the same or similar oxygen content environment as that during collection. Through the in-situ collection mode of this embodiment, the integrity of the indigenous microbiota is maintained, the conditions are easy to control, it is convenient to carry and easy to use, greatly saving the time and cost of field operations.

[0034] Specifically, in this embodiment, the heat preservation box is selected as a thickened food-grade polystyrene resin foam heat preservation box. The external dimensions of the heat preservation box are: length 50 - 60 cm, width 30 - 40 cm, height 20 - 30 cm; the internal dimensions of the heat preservation box are: length 40 - 50 cm, width 20 - 30 cm, height 15 - 25 cm; the density of the heat preservation box is 700 g / m 3 , and the wall thickness is 4 - 5 cm. The heat preservation box includes a heat preservation box body 1 and a heat preservation box cover 2, and the heat preservation box body 1 and the heat preservation box cover 2 are detachably connected.

[0035] This embodiment further includes a collection box, which is located in the heat preservation box. A collection cavity is formed in the collection box, and the culture box is located in the collection cavity. The collection box is made of 304-type food-grade stainless steel. The external dimensions of the collection box are: length 38 - 48 cm, width 18 - 28 cm, height 13 - 23 cm. The collection box includes a collection box body and a collection box cover, and the collection box body and the collection box cover are detachably connected.

[0036] In this embodiment, the temperature sensor 9, the humidity sensor 10, and the oxygen sensor 11 are all located inside the capture box.

[0037] The tray in this embodiment is made of 304 - type food - grade stainless steel or food - grade plastic. The dimensions of the tray are: length 18 - 20 cm, width 12 - 14 cm, height 5 - 8 cm. The tray is located inside the capture cavity. The length and width of the culture box are smaller than those of the tray, and there is at least a 5 - cm gap between the top of the culture box and the capture box cover to facilitate air circulation. There is a gap between the tray and the inner wall of the capture box.

[0038] In this embodiment, there is a heat - insulation structure between the bottom of the tray and the capture box. The heat - insulation structure is located at the four corners of the bottom of the tray and is made of food - grade foam pads to facilitate air circulation and control heat conduction.

[0039] In this embodiment, the bottom of the culture box is provided with through - holes. The culture box is made of a disposable bio - based degradable environmental - protection lunch box (rice - husk lunch box, corn - starch lunch box, wheat - straw lunch box); or, it is a water - draining type 304 stainless - steel water - draining basket or food - grade water - draining basket with through - holes opened at the bottom; or, it is an existing special microbial culture vessel.

[0040] In this embodiment, the temperature module includes a refrigeration module 7 and a heating module 8. This embodiment also includes a thermostat 3. The thermostat 3 includes a heating switch 5, a refrigeration switch 6, and a temperature display. The heating switch 5 is electrically connected to the heating module 8, and the refrigeration switch 6 is electrically connected to the refrigeration module 7. The temperature control range of the thermostat 3 is 10 - 30 °C, and the control accuracy is ±2 °C. Through the temperature collected by the temperature sensor 9, the heating switch 5 or the refrigeration switch 6 is controlled to achieve heating or refrigeration until the required temperature is reached.

[0041] This embodiment also includes a battery 12. The battery 12 is preferably a 12V, 50Ah lithium iron phosphate battery, which can be charged using a car on - vehicle charger or charged using 220V indoors. After being fully charged, it can supply power to the load for 8 - 10 hours.

[0042] This embodiment also includes a monitor 4, which is used to display the measured temperature, humidity, and oxygen content. The humidity is controlled at a relative humidity of 60 - 70%. When the humidity is lower than this range, a hand - held sprayer is used to spray mineral water or pure water for humidification. The oxygen content is controlled at 6 - 21%. When the oxygen content is lower than 6%, the capture box cover is opened to briefly ventilate and supplement oxygen.

[0043] The portable in-situ indigenous microbiome trap of this embodiment includes a heat preservation box, a trapping box, a temperature module, a battery 12, a temperature sensor 9, a humidity sensor 10, an oxygen sensor 11, etc. The total weight does not exceed 4 Kg, it is light and convenient to carry, can be vehicle-mounted, and is convenient for field operations; it is convenient to control environmental conditions such as temperature, humidity, and oxygen content, and is as similar as possible to the environmental conditions of the regional indigenous microbiome to ensure that the trapped indigenous microbiome is maximally similar to the real situation; it changes in-situ trapping to ex-situ trapping, greatly shortening the field operation time from 5 - 10 days to about 1 - 2 hours, and changing from at least 2 field operations to 1 field operation; the materials selected in this embodiment are environmentally friendly and do not pose a threat to the growth of microorganisms; the production materials of this embodiment are cheap and easy to obtain, the usage method is simple, easy to operate, and greatly saves costs; a lithium iron phosphate battery 12 is selected as the portable charging power supply, which is convenient for charging and field use; a heating semiconductor is used to form a heating module 8, and a refrigeration semiconductor refrigeration module 7, which is small in size, light in weight, high in efficiency, stable in function, and convenient to control.

[0044] The present invention aims at the improvement of the device structure, and the control process is the prior art.

[0045] Prototype production of the portable in-situ indigenous microbiome trap of this embodiment:

[0046] (1) Heat preservation box: Select a thickened food-grade polystyrene resin foam heat preservation box, with external dimensions: length 60 cm, width 38 cm, height 28 cm; internal dimensions: length 47 cm, width 26 cm, height 21 cm. The density is 700 g, the heat preservation box cover is 5 cm thick, and the wall thickness is 4 cm;

[0047] (2) Trapping box: Select a 304-type food-grade stainless steel box, with length 29.5 cm, width 23.5 cm, height 20 cm, and a 304 stainless steel cover;

[0048] (3) Tray: Select a 304-type food-grade stainless steel square tray, with length 20 cm, width 12 cm, height 5 cm;

[0049] (4) Battery 12: Select a rechargeable lithium iron phosphate battery, 12V, 50Ah;

[0050] (5) Heating module 8: Select a heating semiconductor model 12706, 12V, 60W;

[0051] Refrigeration module 7: Refrigeration semiconductor model 12706, 12V, 60W, electric fan 12V 5W;

[0052] (6) Measurement range of the temperature sensor 9: -30 - 70 °C ± 2 °C, measurement range of the humidity sensor 10: 0 - 100% ± 1%, measurement range of the oxygen sensor: 0 - 30% VOL;

[0053] (7) Select a thermostat 3 model: XY-ST10 (10A relay); heating switch 5 model: KCD1; cooling switch 6 model: KCD1;

[0054] (8) Select a monitor 4: SYW-3 model;

[0055] (9) Assemble and connect according to the attached drawings, and use foaming foam glue to connect and seal the relevant parts to make a portable in-situ trap for indigenous microbiota.

[0056] Application Example 1

[0057] Select the Mulinzi Primeval Forest Reserve in Hefeng, Hubei. Collect a mixed sample of rotten leaves and humus-rich soil from the litter layer under the fallen leaves in the forest, and place it in a pre-sterilized tray. The thickness of the sample layer is about 1 cm. Place a washed and disinfected culture box (a disposable corn starch lunch box with microporous hollow at the bottom) on the mixed sample, fill it with pre-steamed organic brown rice to a height of 2 / 3, leaving 1 / 3 space. Cover the culture box with disinfected gauze and fix it with a disinfected rubber band. After the above operations are completed, place it in the constant temperature chamber of the portable in-situ trap for indigenous microbiota for cultivation. The temperature measured at the sampling site is 20 °C and the relative humidity is 70%. Therefore, the temperature of the portable in-situ trap chamber for indigenous microbiota is controlled and set at 20 °C, and a small amount of water is supplemented by spraying to maintain the relative humidity in the trap chamber at about 70%. The portable in-situ trap for indigenous microbiota is placed in a car and brought back indoors, and the battery 12 is charged once every 12 hours. After 5 days of cultivation, a large number of white and pink colonies are observed to form on the rice, and the collection process ends, as Figure 4 shown. Mix the trapped sample with brown sugar at a ratio of 1:1 (by mass) and place it in a washed and disinfected brown bottle, then store it in the refrigerator for subsequent research or the development of microbial agent products.

[0058] Application Example 2

[0059] The original forest reserve of Dashui Forest Farm in Baokang, Hubei Province was selected to collect the mixed samples of rotten leaves and humus-containing soil in the litter layer under the deciduous forest, and put them into a pre-sterilized tray. The sample was laid with a thickness of about 1 cm. A culture box (a disposable corn starch lunch box with microporous hollows at the bottom) that had been cleaned and disinfected was placed on the mixed sample, filled with pre-steamed organic brown rice, filled to a height of 2 / 3, leaving 1 / 3 of the gap, and the culture box was covered with sterilized gauze and fixed with a sterilized rubber band. After the above operation was completed, it was placed in a portable indigenous microbiome ectopic trap constant temperature room for culture. The temperature measured at the sampling site was 19°C and the relative humidity was 70%. Therefore, the temperature control of the portable indigenous microbiome ectopic trap room was set to 19°C, and a small amount of water was added by spraying to maintain the relative humidity of the trap room at about 70%. The portable indigenous microbiome ectopic trap was placed in the car and brought back to the room, and the battery 12 was charged once every 12 hours. After 5 days of culture, a large number of white and pink colonies were observed to form on the rice, and the collection process was completed. The captured sample is thoroughly mixed with brown sugar in a ratio of 1:1 (by mass), placed in a cleaned and sterilized brown bottle, and stored in a refrigerator for subsequent research or microbial product development.

[0060] Application example three:

[0061] Tianmu Mountain Nature Reserve in Lin'an, Zhejiang Province was selected to collect the rotten layer of fallen leaves and their humus-containing soil mixed samples under the bamboo forest, and put them into a pre-sterilized tray with a sample laying thickness of about 1 cm. A cleaned and disinfected culture box (a disposable environmentally friendly straw lunch box with microporous hollows at the bottom) was placed on the mixed sample, filled with pre-steamed organic brown rice, filling 2 / 3 of the height, leaving 1 / 3 of the gap, and the culture box was covered with sterilized gauze and fixed with a sterilized rubber band. After the above operations were completed, the portable indigenous microbiome ectopic trap was placed in a constant temperature chamber for culture. The temperature measured at the sampling site was 22°C and the relative humidity was 75%. Therefore, the temperature control of the portable indigenous microbiome ectopic trap chamber was set to 22°C, and a small amount of water was added by spraying to maintain the relative humidity of the trap chamber at about 75%. The portable indigenous microbiome ectopic trap was placed in the car and brought back indoors, and the battery 12 was charged once every 12 hours. After 5 days of culture, a large number of white and pink colonies were observed to form on the rice, and the collection process was completed. Figure 5 The captured sample was mixed with brown sugar in a ratio of 1:1 (by mass) and then placed in a cleaned and sterilized brown bottle and stored in a refrigerator for subsequent research or development of microbial products.

[0062] Application example 4:

[0063] Select an organic agricultural park in Suzhou, collect surface soil samples, and place them in a pre-sterilized tray. The thickness of the sample layer is about 1 cm. Place a washed and disinfected culture box (a disposable environmentally friendly corn starch lunch box with microporous hollowing at the bottom) on the mixed sample, fill it with pre-steamed organic brown rice to a height of 2 / 3, leaving 1 / 3 space. Cover the culture box with disinfected gauze and fix it with a disinfected rubber band. After the above operations are completed, place it in the constant temperature chamber of the portable indigenous microbiome off-site collector for cultivation. The temperature measured at the sampling site is 18°C, and the relative humidity is 70%. Therefore, the temperature of the portable indigenous microbiome off-site collection chamber is controlled and set at 18°C, and a small amount of water is supplemented by spraying to maintain the relative humidity in the collection chamber at about 70%. The portable indigenous microbiome off-site collector is placed in a car and taken back indoors, and the battery is charged every 12 hours. After 9 days of cultivation, a large number of white and pink colonies are observed on the rice, and the collection process ends. Mix the collected sample with brown sugar at a ratio of 1:1 (by mass) and place it in a washed and disinfected brown bottle, then store it in the refrigerator for subsequent research or the development of microbial agent products.

[0064] In the present utility model, specific examples are used to elaborate on the principle and implementation mode of the present utility model. The description of the above embodiments is only used to help understand the method and its core idea of the present utility model; at the same time, for those of ordinary skill in the art, according to the idea of the present utility model, there will be changes in the specific implementation mode and application scope. In summary, the content of this specification should not be construed as a limitation to the present utility model.

Claims

1. A portable ex situ trap for indigenous microorganisms, characterized by: include: An incubator, a tray, a culture box, a temperature module, a temperature sensor, a humidity sensor and an oxygen sensor, wherein the culture box and the tray are arranged in the incubator, the tray is used to hold samples, the culture box is used to be placed on the samples on the tray, the temperature module is used to provide the required temperature for microorganisms, the temperature sensor is used to detect the temperature of the environment where the culture box is located, the humidity sensor is used to detect the humidity of the environment where the culture box is located, and the oxygen sensor is used to detect the oxygen content of the environment where the culture box is located.

2. The portable ex situ trap for indigenous microorganisms according to claim 1, characterized in that: It also includes a capture box, which is located in the incubator. A capture cavity is formed in the capture box, and the culture box is located in the capture cavity.

3. The portable ex situ trap for indigenous microorganisms according to claim 2, characterized in that: The temperature sensor, the humidity sensor and the oxygen sensor are all located in the capture box.

4. The portable ex situ trap for indigenous microorganisms according to claim 2, characterized in that: The tray is located in the capture cavity, and there is a gap between the tray and the inner wall of the capture box.

5. The portable ex situ trap for indigenous microorganisms according to claim 4, characterized in that: There is a heat insulation structure between the bottom of the tray and the capture box.

6. The portable ex situ trap for indigenous microorganisms according to claim 1, characterized in that: The heat preservation box comprises a heat preservation box body and a heat preservation box cover, and the heat preservation box body and the heat preservation box cover are detachably connected.

7. The portable ex situ trap for indigenous microorganisms according to claim 2, characterized in that: The capture box is made of stainless steel and comprises a capture box body and a capture box cover, wherein the capture box body and the capture box cover are detachably connected.

8. The portable ex situ trap for indigenous microorganisms according to claim 1, characterized in that: The temperature module includes a cooling module and a heating module.

9. The portable ex situ trap for indigenous microorganisms according to claim 1, characterized in that: The bottom of the culture box is provided with a through hole.

10. The portable ex situ trap for indigenous microorganisms according to claim 1, characterized in that: Also includes batteries.

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