Device for enzyme biotransformation

Abstract

The utility model relates to the field of biotransformation, in particular to a device for enzyme biotransformation, which comprises a transformation box, the transformation box is arranged on a placing frame, a sealing upper cover is arranged at the top of the transformation box, a motor is arranged in the middle of the sealing upper cover, a through hole is arranged on the upper surface of the sealing upper cover and positioned on one side of the motor, and an enzyme medicament needle cylinder is arranged at the through hole. A pair of mounting frames are arranged on a sealing upper cover of a conversion box, enzyme medicament needle cylinders can be effectively mounted on the mounting frames, the enzyme medicament needle cylinders can be mounted and dismounted conveniently and quickly only by rotating a limiting plate, a piston is pushed through cooperation of a servo electric cylinder and movement of a telescopic rod, and therefore quantitative enzyme extrusion can be achieved; therefore, the extending length of the telescopic rod can be accurately controlled, the enzyme extrusion amount can be accurately controlled, the method is more accurate, an operator can clearly know the enzyme extrusion amount, and waste is effectively reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of biotransformation, in particular to a device for enzymatic biotransformation. Background Art

[0002] Biotransformation refers to the chemical reaction process in an organism, which can convert one substance into another. There are many ways of biotransformation, and four of the more common ones are enzymatic catalysis, fermentation, photosynthesis, and respiration.

[0003] Among them, the utility model with the authorization announcement number CN214032517U discloses a device for the biotransformation of glucosyl steviol glycoside enzyme. Although through the cooperation of the feed hopper, mounting holes, support plates, fixing plates, telescopic rods, sliding holes, connecting blocks, connecting rods, fixing blocks, baffles, linkage blocks, limiting sleeves, positioning holes, and positioning blocks, the connecting block can be driven to move by operating the telescopic rod, thereby controlling the connecting rod to drive the baffle to deflect, controlling the gap between the two baffles, controlling the feeding speed of the enzyme, and thus controlling the reaction progress during the transformation process, ensuring full transformation between the enzyme and the mother liquor sugar, and preventing unnecessary waste or insufficient transformation. However, this method cannot accurately control the feeding amount of the enzyme, and the operator cannot clearly know the feeding amount. Summary of the Invention

[0004] The purpose of the utility model is to solve the deficiencies existing in the prior art, and a device for enzymatic biotransformation is proposed.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] A device for enzymatic biotransformation includes a conversion tank, which is arranged on a placement rack. A sealing upper cover is arranged on the top of the conversion tank. A motor is arranged in the middle of the sealing upper cover. A through hole is opened on the upper surface of the sealing upper cover on one side of the motor. An enzyme reagent syringe is arranged at the through hole. Mounting racks are symmetrically arranged on both sides of the through hole on the upper surface of the sealing upper cover. The enzyme reagent syringe is installed between the two mounting racks. A servo electric cylinder is arranged on the upper surface of the sealing upper cover on one side of the mounting rack;

[0007] An installation groove is opened on the upper surface of the plate body at the upper end of the mounting rack. A rotating seat is arranged on the upper surface of the mounting rack on one side of the installation groove. A limiting plate is rotatably arranged on the rotating seat;

[0008] A convex block is arranged on the upper surface of the plate body of the limiting plate, and a rubber pad is attached to the lower surface of the plate body of the limiting plate.

[0009] In addition, the preferred structure is that convex plates are symmetrically connected to both side walls at the top of the barrel of the enzyme reagent syringe, and a piston is arranged in the inner cavity of the barrel of the enzyme reagent syringe.

[0010] In addition, a preferred structure is that the convex plate can be installed into the installation groove, the limiting plate can be rotated above the installation groove, and the rubber pad can contact the upper surface of the convex plate.

[0011] In addition, a preferred structure is that a telescopic rod is provided at the bottom output end of the servo electric cylinder, and the telescopic rod and the piston are located at the same center.

[0012] In addition, a preferred structure is that a stirring rod is provided at the bottom output end of the motor, and the stirring rod is arranged in the inner cavity of the conversion box.

[0013] In addition, a preferred structure is that a feed inlet is provided on the upper surface of the sealing upper cover on the other side of the motor away from the mounting bracket.

[0014] The beneficial effects of the present utility model are as follows: By providing a pair of mounting brackets on the sealing upper cover of the conversion box, the mounting brackets can effectively mount the enzyme reagent syringe, and the installation and disassembly method of the enzyme reagent syringe is relatively convenient. Only by rotating the limiting plate, the piston can be pushed by the movement of the servo electric cylinder in cooperation with the telescopic rod, so as to realize quantitative extrusion of the enzyme. In this way, the length of the telescopic rod extending out can be accurately controlled, and thus the amount of the enzyme extruded can be accurately controlled. Such a method is more accurate, and the operator can also clearly know the amount of the enzyme extruded, effectively reducing waste. Description of the Drawings

[0015] Figure 1 It is a schematic structural diagram of a biological conversion device;

[0016] Figure 2 It is a schematic structural diagram of the conversion box;

[0017] Figure 3 It is a schematic structural diagram of the sealing upper cover;

[0018] Figure 4 It is a schematic structural diagram after the enzyme reagent syringe is separated from the mounting bracket;

[0019] Figure 5 It is a schematic structural diagram of the limiting plate;

[0020] Figure 6 It is a schematic structural diagram of the telescopic rod extending into the enzyme reagent syringe 6.

[0021] In the figure: 1 conversion box, 2 sealing upper cover, 21 through hole, 22 feed inlet, 3 placement rack, 4 servo electric cylinder, 41 telescopic rod, 5 mounting bracket, 51 installation groove, 52 rotating seat, 53 limiting plate, 531 rubber pad, 532 convex block, 6 enzyme reagent syringe, 61 convex plate, 62 piston, 7 motor, 8 stirring rod. Detailed Description of the Invention

[0022] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments.

[0023] Referring to Figure 1-6 , a device for enzymatic biotransformation includes a conversion tank 1, the conversion tank 1 is arranged on a placement rack 3, a sealing upper cover 2 is arranged on the top of the conversion tank 1, a motor 7 is arranged in the middle of the sealing upper cover 2, a through hole 21 is opened on the upper surface of the sealing upper cover 2 on one side of the motor 7, an enzyme reagent syringe 6 is arranged at the through hole 21, mounting frames 5 are symmetrically arranged on the upper surface of the sealing upper cover 2 on both sides of the through hole 21, the enzyme reagent syringe 6 is installed between the two mounting frames 5, and a servo electric cylinder 4 is arranged on the upper surface of the sealing upper cover 2 on one side of the mounting frame 5;

[0024] Among them, a mounting groove 51 is opened on the upper surface of the plate body at the upper end of the mounting frame 5, a rotating seat 52 is arranged on the upper surface of the mounting frame 5 on one side of the mounting groove 51, a limiting plate 53 is rotatably arranged on the rotating seat 52, and the mounting groove 51 is convenient for installing the enzyme reagent syringe 6, so as to play a positioning role.

[0025] In addition, a convex block 532 is arranged on the upper surface of the plate body of the limiting plate 53, and a rubber pad 531 is attached to the lower surface of the plate body of the limiting plate 53. The rubber pad 531 can be used to tightly fit the enzyme reagent syringe 6 to prevent it from loosening.

[0026] In addition, convex plates 61 are symmetrically connected and arranged on both side walls at the top of the barrel of the enzyme reagent syringe 6, a piston 62 is arranged in the inner cavity of the barrel of the enzyme reagent syringe 6, the convex plates 61 can be installed into the mounting groove 51, and the limiting plate 53 can be rotated above the mounting groove 51, and the rubber pad 531 can contact the upper surface of the convex plates 61. The convex plates 61 play an effective limiting role and can fix the enzyme reagent syringe 6 on the mounting frame 5.

[0027] Among them, a telescopic rod 41 is arranged at the bottom output end of the servo electric cylinder 4, and the telescopic rod 41 and the piston 62 are located at the same center. The telescopic rod 41 is convenient for pushing against the piston 62 to push it downward, so as to squeeze out the enzyme reagent in the enzyme reagent syringe 6.

[0028] Moreover, a stirring rod 8 is arranged at the bottom output end of the motor 7, the stirring rod 8 is arranged in the inner cavity of the conversion tank 1, and the stirring rod 8 can perform stirring.

[0029] In addition, a feed inlet 22 is arranged on the upper surface of the sealing upper cover 2 on the other side of the motor 7 away from the mounting frame 5, and the feed inlet 22 is used for pouring raw materials.

[0030] In this embodiment, when performing enzyme-related biotransformation, first install the enzyme reagent syringe 6 filled with enzyme into the mounting rack 5. The telescopic rod 41 of the servo cylinder 4 is in a non-extended state. At this time, there is enough space to facilitate the installation of the enzyme reagent syringe 6. At this time, the enzyme reagent syringe 6 can be vertically inserted into the through hole 21, and the convex plates 61 on both sides are installed into the mounting grooves 51. Then, the limiting plate 53 can be rotated by pinching the convex block 532, so that the limiting plate 53 abuts above the convex plate 61 to prevent it from disengaging from the mounting groove 51. And the rubber pad 531 fits against the convex plate 61. The rubber pad 531 can ensure that it fits against the convex plate 61 without scratching the convex plate 61.

[0031] After the enzyme reagent syringe 6 is installed, enzyme biotransformation can be carried out. The telescopic rod 41 at the bottom of the servo cylinder 4 extends out. While abutting against the piston 62, it continues to push downward. The enzyme inside is extruded by the downward movement of the piston 62 and is extruded into the inside of the conversion tank 1. Then, the raw materials are poured in from the feed port 22, and the stirring rod 8 is driven by the motor 7 to stir and mix.

[0032] Moreover, the servo cylinder 4 can provide more accurate positioning and rapid real-time feedback. And it can be known in advance through testing how much enzyme is extruded corresponding to each section of the telescopic rod 41 extending out. In this way, the length of the telescopic rod 41 extending out can be accurately controlled, so as to accurately control the amount of enzyme extruded.

[0033] In this utility model, by providing a pair of mounting racks 5 on the sealed upper cover 2 of the conversion tank 1, the mounting rack 5 can effectively install the enzyme reagent syringe 6, and the installation and disassembly method of the enzyme reagent syringe 6 is relatively convenient. Only the limiting plate 53 needs to be rotated. By the cooperation of the servo cylinder 4 and the telescopic rod 41 to push the piston 62, quantitative extrusion of the enzyme can be achieved. In this way, the length of the telescopic rod 41 extending out can be accurately controlled, so as to accurately control the amount of enzyme extruded. Such a method is more accurate, and the operator can also clearly know the amount of enzyme extruded, effectively reducing waste.

[0034] The above is only a preferred specific embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present utility model, according to the technical solution and the inventive concept of the present utility model, making equivalent replacements or changes should be covered within the protection scope of the present utility model.

Claims

1. A device for enzymatic bioconversion, comprising a conversion box (1), the conversion box (1) being arranged on a placement rack (3), characterized in that: The conversion box (1) is provided with a sealed upper cover (2) on the top, a motor (7) is provided in the middle of the sealed upper cover (2), a through hole (21) is provided on the upper surface of the sealed upper cover (2) on one side of the motor (7), an enzyme agent syringe (6) is provided at the through hole (21), mounting frames (5) are symmetrically provided on both sides of the through hole (21) on the upper surface of the sealed upper cover (2), the enzyme agent syringe (6) is installed between the mounting frames (5) on both sides, and a servo electric cylinder (4) is provided on the upper surface of the sealed upper cover (2) on one side of the mounting frame (5); A mounting groove (51) is provided on the upper surface of the plate body at the upper end of the mounting frame (5); a rotating seat (52) is provided on the upper surface of the mounting frame (5) at one side of the mounting groove (51); a limit plate (53) is rotatably provided on the rotating seat (52); A convex block (532) is provided on the upper surface of the limit plate (53), and a rubber pad (531) is attached to the lower surface of the limit plate (53).

2. A device for enzymatic bioconversion according to claim 1, characterized in that: The side walls on both sides of the top of the enzyme medicine syringe (6) are symmetrically connected to each other and are provided with convex plates (61), and the inner cavity of the enzyme medicine syringe (6) is provided with a piston (62).

3. A device for enzymatic bioconversion according to claim 2, characterized in that: The convex plate (61) can be installed in the installation groove (51), and the limiting plate (53) can be rotated to the top of the installation groove (51), and the rubber pad (531) can contact the upper surface of the convex plate (61).

4. The device for enzymatic bioconversion according to claim 1, characterized in that: A telescopic rod (41) is provided at the bottom output end of the servo electric cylinder (4), and the telescopic rod (41) and the piston (62) are located at the same center.

5. The device for enzymatic bioconversion according to claim 1, characterized in that: The bottom output end of the motor (7) is provided with a stirring rod (8), and the stirring rod (8) is arranged in the inner cavity of the conversion box (1).

6. The device for enzymatic bioconversion according to claim 1, characterized in that: A feed port (22) is provided on the upper surface of the sealing upper cover (2) at the other side of the motor (7) away from the mounting frame (5).

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