Dual-channel portable fluorescence detector

Abstract

The utility model relates to the technical field of fluorescence detection instruments, in particular to a double-channel portable fluorescence detector. Comprising a lithium battery, a transformer, an electromagnetic relay, a constant current driving module, a cross light path module, a current-voltage conversion board and an analog-to-digital converter, and the lithium battery supplies power to an internal circuit of the detector; the transformer intelligently converts the power supply voltage of the lithium battery; the electromagnetic relay is connected with the constant-current driving module and drives the LED light source to be lightened in a pulse mode. The cross light path module comprises an LED light source, a silicon photocell and a light filter, and the LED light source and the silicon photocell are fixed through a buckle; the current-voltage conversion plate is connected with the silicon photocell, converts a photocurrent signal into a voltage signal, amplifies the voltage signal and inputs the amplified voltage signal into the analog-to-digital converter; the analog-to-digital converter converts the received voltage signal into a digital signal. According to the cross-shaped light path, double-channel fluorescence detection under double excitation wavelengths can be achieved, the detection flexibility is greatly improved, and the application range is greatly widened.

Description

Technical Field

[0001] The utility model relates to the technical field of fluorescence detection instruments, in particular to a dual-channel portable fluorescence detector. Background Technique

[0002] Fluorescence detection technology is a common detection technology in biochemical detection and analysis. This technology utilizes the characteristic that certain detection samples produce fluorescence after being activated, and uses a fluorescence detector to conduct qualitative or quantitative analysis on the detection samples.

[0003] The existing fluorescence detection technology relies on a fixed fluorescence spectrometer in the laboratory to measure the fluorescence response between pollutants and specific fluorescence probes. This process involves using a computer to generate a standard curve, and then converting the fluorescence signal into the pollutant concentration. However, this method has the problems of long time consumption and high cost. Especially when facing volatile or unstable pollutants, it is difficult to achieve instant detection and cannot meet the urgent need for rapid on-site screening of various pollutants. Currently, the portable fluorescence detection devices on the market generally rely on external ultraviolet light sources and rely on mobile phone cameras to capture fluorescence signals. Although the cost is well controlled, they face the problem of poor stability and require frequent calibration after changing the device or the detection environment. In addition, most of these devices use a fixed 365-nanometer wavelength light source, which limits their compatibility with diverse fluorescence probes and cannot adapt to the excitation requirements of different probes by simply changing the light source. Content of the Utility Model

[0004] In order to solve the problems existing in the prior art, the utility model provides a dual-channel portable fluorescence detector, including: a lithium battery, a transformer, an electromagnetic relay, a constant current drive module, a cross optical path module, a current-voltage conversion board, and an analog-to-digital converter. The lithium battery supplies power to the internal circuit of the detector; the transformer intelligently converts the power supply voltage of the lithium battery; the electromagnetic relay is connected to the constant current drive module to drive the LED light source to be lit in a pulsed manner; the cross optical path module includes an LED light source, a silicon photocell, and a filter. The LED light source and the silicon photocell are fixed by a buckle; the current-voltage conversion board is connected to the silicon photocell, converts the photocurrent signal into a voltage signal, amplifies it, and then inputs it into the analog-to-digital converter; the analog-to-digital converter converts the received voltage signal into a digital signal. The cross-shaped optical path of this application can realize dual-channel fluorescence detection under dual excitation wavelengths, greatly enhancing the flexibility and application range of detection.

[0005] The utility model adopts the following technical scheme. A dual-channel portable fluorescence detector includes: a lithium battery, a transformer, an electromagnetic relay, a constant current drive module, a cross optical path module, a current-voltage conversion board, and an analog-to-digital converter. Specifically:

[0006] The lithium battery is used to supply power to the internal circuit of the detector;

[0007] The transformer is used for intelligently converting the power supply voltage of the lithium battery;

[0008] The electromagnetic relay is connected to the constant current drive module and is used for driving the LED light source to be lit in a pulsed manner;

[0009] The cross optical path module includes an LED light source, a silicon photocell and a filter. The LED light source and the silicon photocell are fixed on the bracket of the cross optical path module through buckles;

[0010] The current-voltage conversion board is connected to the silicon photocell and is used for converting the photocurrent signal into a voltage signal, amplifying the voltage signal, and inputting the amplified signal into an analog-to-digital converter;

[0011] The analog-to-digital converter is used for converting the received voltage signal into a digital signal.

[0012] Further, the cross optical path module contains two LED light sources and two silicon photocells, and the LED light sources and the silicon photocells are arranged in a cross shape.

[0013] Further, the LED light source is driven by the constant current drive module, and the supported excitation wavelength range is from 270 nm to 525 nm.

[0014] Further, the cross optical path module contains four filters, which are used for filtering the excitation light of the LED light source and the emitted light of the silicon photocell to prevent the silicon photocell from receiving the optical signal excited by the LED light source.

[0015] Further, the fluorescence detector further includes a communication module. The communication module includes a USB communication module and a Bluetooth communication module. The USB communication module is used for sending the digital signal to a computer terminal in a wired manner; the Bluetooth communication module is used for sending the digital signal to a mobile phone terminal through a Bluetooth wireless connection method.

[0016] The beneficial effects of the present utility model are as follows: The cross-shaped optical path module proposed in the present utility model realizes dual-channel fluorescence detection under double excitation wavelengths by integrating precise optical components with interchangeable light sources and filters, effectively enhancing the flexibility and applicable range of detection. And a photoelectric sensor is arranged inside the instrument, which can convert the received fluorescence signal into an electrical signal, and use Bluetooth or USB technology to instantly transmit the signal to a mobile phone or other intelligent terminal devices. With the corresponding application program, users can view, process and analyze the detection data in real time, improving the detection efficiency and convenience. Description of the Drawings

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0018] Figure 1 Structural schematic diagram of a dual-channel portable fluorescence detector in an embodiment of the present application;

[0019] Figure 2 Structural schematic diagram of the upper cover of the dual-channel portable fluorescence detector in an embodiment of the present application;

[0020] Figure 3 Top view of the dual-channel portable fluorescence detector in an embodiment of the present application;

[0021] Figure 4 Front view of the dual-channel portable fluorescence detector in an embodiment of the present application;

[0022] Figure 5 Rear view of the dual-channel portable fluorescence detector in an embodiment of the present application;

[0023] Figure 6 Side view of the dual-channel portable fluorescence detector in an embodiment of the present application;

[0024] Figure 7 Magnified schematic diagram of the cross optical module of the dual-channel portable fluorescence detector in an embodiment of the present application;

[0025] Figure 8 Schematic diagram of the detection result of the hydrolysis product 3-phenoxybenzaldehyde of pyrethroid pesticides detected by the dual-channel portable fluorescence detector in an embodiment of the present application;

[0026] Figure 9 Schematic diagram of the detection result of tetracycline antibiotics detected by the dual-channel portable fluorescence detector in an embodiment of the present application;

[0027] In the figure, reference numeral 1. light-shielding cover; 2. cuvette slot; 3. lithium battery; 4. transformer; 5. electromagnetic relay; 6. switch; 7. cross optical module; 8. constant current drive module; 9. cooling fan; 10. I / V conversion board; 11. USB-C communication module; 12. analog-to-digital converter; 13. Bluetooth communication module; 14. USB-C interface; 15. power display window; 16. light source switch; 17. DC interface; 18. heat dissipation window; 19. heat dissipation window; 71. silicon photocell buckle; 72. LED light source buckle; 73. filter slot; 74. filter slot. Detailed implementation manners

[0028] The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts shall fall within the protection scope of the present utility model.

[0029] A structural schematic diagram of a dual-channel portable fluorescence detector according to an embodiment of the present utility model is as Figure 1 shown, including: a lithium battery, a transformer, an electromagnetic relay, a constant current drive module, a cross optical path module, a current-voltage conversion board, and an analog-to-digital converter, wherein: the lithium battery is used to supply power to the internal circuit of the detector; the transformer is used to intelligently convert the power supply voltage of the lithium battery; the electromagnetic relay is connected to the constant current drive module and is used to drive the LED light source to be lit in a pulsed manner; the cross optical path module includes an LED light source, a silicon photocell, and a filter, and the LED light source and the silicon photocell are fixed on the bracket of the cross optical path module by a buckle; the current-voltage conversion board is connected to the silicon photocell and is used to convert the photocurrent signal into a voltage signal and amplify it, and input the amplified signal into the analog-to-digital converter; the analog-to-digital converter is used to convert the received voltage signal into a digital signal.

[0030] The fluorescence detector further includes a communication module, and the communication module includes a USB communication module and a Bluetooth communication module. The USB communication module is used to send the digital signal to the computer terminal in a wired manner; the Bluetooth communication module is used to send the digital signal to the mobile phone terminal by Bluetooth wireless connection.

[0031] Specifically, as Figure 2 - 6As shown, when using the dual-channel fluorescence detector proposed in this application for detection, first combine the upper cover with the device, insert the cuvette into the cuvette slot 2 and close the light-shielding cover 1; turn on the switch 6, and after placing the light source switch 16 in the on position, the instrument starts to measure; the circuit in this fluorescence detector is powered by a lithium battery 3, and its remaining power can be observed through the power display window 15, and at the same time, power supply replacement or charging is achieved through the DC interface 17; the transformer 4 reduces the 12V voltage provided by the lithium battery to 5V and supplies power to the I / V converter 10, the USB-C communication module 11, the analog-to-digital converter 12, and the Bluetooth communication module 13, and at the same time supplies 12V DC power to the electromagnetic relay 5 and the cooling fan 9; the electromagnetic relay 5 is connected to two constant current drive modules 8, enabling each to drive an LED light source. When the light source switch 16 is turned on, the electromagnetic relay 5 operates at a fixed frequency, causing the LED light source to be lit in a pulsed manner, which can avoid overheating and increase the device usage time; two silicon photocells are respectively connected to the I / V conversion board 10, thereby converting the photocurrent signal into a voltage signal and amplifying it by 100 to 200,000 times, and then inputting it into the analog-to-digital converter 12 to be converted into a digital signal. The signal can be sent to a computer, mobile phone, or other intelligent device terminals in a wired manner or through a wireless Bluetooth connection through the USB-C communication module 11 and the Bluetooth communication module 13 connected to the analog-to-digital converter 12.

[0032] Since traditional portable fluorescence detectors cannot detect two emission wavelengths simultaneously, and a desktop fluorescence spectrometer takes more than 10 seconds to measure a set of data when scanning the entire band for dual-wavelength measurement; based on the design principle that the excitation light source of fluorescence detection must be perpendicular to the detector, this application has developed a cross-shaped optical path module with dual excitation light sources and dual detectors, which can provide up to two-way excitation and two-channel simultaneous detection. Due to having two high-power LED light sources, different excitation wavelengths can be provided simultaneously, which is suitable for the detection of multi-component fluorescence probes with different excitations; as Figure 7 shown, this optical module fixes the silicon photocell and the LED light source on the module bracket through the 71. silicon photocell buckle and the 72. LED light source buckle, so different-sensitivity silicon photocells and LED light sources with different wavelengths can be replaced at any time, which is convenient for adjustment according to different fluorescence probe systems. At the same time, the filter can be inserted into the 73. filter slot and the 74. filter slot to achieve the filtering of the excitation light and the emission light, and prevent the silicon photocell from receiving the light signal of the LED excitation light source.

[0033] In another specific embodiment of this application, when using a fluorescence probe to detect m-phenoxybenzaldehyde, the hydrolysis product of pyrethroid pesticides, when the concentration of m-phenoxybenzaldehyde is in the range of 0 - 20 μM, the concentration obtained by the fluorescence detection instrument proposed in this application has a good linear relationship with the electrical signal intensity, as Figure 8As shown, its linear equation is y = -0.0276x + 1.4988, R2 = 0.9983, and the detection limit is 0.770 μM.

[0034] In another specific embodiment of the present application, when using the fluorescent probe to detect tetracycline antibiotics, when the concentration of tetracycline hydrochloride is in the range of 0 - 75 μM, a good linear relationship exists between the concentration obtained by the fluorescence detection instrument proposed in the present application and the electrical signal intensity, as Figure 9 shown, its linear equation is y = 0.0055x + 0.8000, R2 = 0.9957, and the detection limit is 0.396 μM.

[0035] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A dual-channel portable fluorescence detector, comprising: A lithium battery, a transformer, an electromagnetic relay, a constant current drive module, a cross optical path module, a current-voltage conversion board, and an analog-to-digital converter, characterized in that: The lithium battery is used to power the internal circuit of the detector; The transformer is used to intelligently convert the power supply voltage of the lithium battery; The electromagnetic relay is connected to the constant current driving module and is used to drive the LED light source to light up in a pulsed manner; The cross light path module comprises an LED light source, a silicon photocell and a filter, and the LED light source and the silicon photocell are fixed on the bracket of the cross light path module by buckles; The current-voltage conversion board is connected to the silicon photocell and is used to convert the photocurrent signal into a voltage signal and amplify it, and input the amplified signal into an analog-to-digital converter; The analog-to-digital converter is used to convert the received voltage signal into a digital signal.

2. A dual-channel portable fluorescence detector according to claim 1, characterized in that: The cross light path module includes two LED light sources and two silicon photocells, and the LED light sources and the silicon photocells are arranged in a cross shape.

3. A dual-channel portable fluorescence detector according to claim 1, characterized in that: The LED light source is driven by the constant current driving module, and supports an excitation wavelength range of 270 nm to 525 nm.

4. A dual-channel portable fluorescence detector according to claim 1, characterized in that: The cross optical path module includes four filters for filtering the excitation light of the LED light source and the emission light of the silicon photocell to prevent the silicon photocell from receiving the light signal excited by the LED light source.

5. A dual-channel portable fluorescence detector according to claim 1, characterized in that: The fluorescence detector also includes a communication module, which includes a USB communication module and a Bluetooth communication module. The USB communication module is used to send the digital signal to a computer via a wired method; the Bluetooth communication module is used to send the digital signal to a mobile phone via a Bluetooth wireless connection.

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