Micro-fluidic chip

Abstract

The utility model relates to the technical field of medical detection, in particular to a micro-fluidic chip. The micro-fluidic chip comprises the detection plate, the first sealing piece, the second sealing piece and the two third sealing pieces, and the detection plate comprises the detection body and the two detection units arranged on the detection body, so that two different samples can be injected into the two detection units, and the detection efficiency is improved. Meanwhile, the first sealing piece and the second sealing piece are respectively positioned on two sides of the accommodating cavity for sealing, so that the subsequent amplification device can simultaneously heat the surfaces of two sides of the accommodating cavity, the PCR reaction time is shortened, the nucleic acid detection time is further shortened, the interior of the cavity is uniformly heated, the temperature difference in the liquid is balanced, and the accuracy of a detection result is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of medical detection, in particular to a microfluidic chip. Background Art

[0002] At present, the kits used for real-time fluorescence PCR (Real-time PCR) detection generally adopt the form of EP tubes, with slow heat conduction, which will cause a long PCR amplification time and poor uniformity of heat received by the sample reagents in the tubes, thus affecting the accuracy of subsequent optical measurement results.

[0003] In addition, for the existing microfluidic chips, a single chip can only detect a single type of sample, resulting in low sample detection efficiency. Summary of the Utility Model

[0004] (I) Technical Problems to be Solved

[0005] In view of the above-mentioned disadvantages and deficiencies of the prior art, the utility model provides a microfluidic chip, which solves the technical problems of inaccurate detection results of the microfluidic chip and slow detection efficiency for multiple samples.

[0006] (II) Technical Solutions

[0007] In order to achieve the above object, the main technical solutions adopted by the utility model include:

[0008] An embodiment of the utility model provides a microfluidic chip, which includes a detection plate, a first sealing sheet, a second sealing sheet and two third sealing sheets; the detection plate includes a detection body and two detection units formed on the detection body, and the two detection units are arranged in mirror symmetry; each detection unit includes a sample injection cavity, a liquid inlet channel, a plurality of accommodation cavities and a plurality of exhaust holes, the sample injection cavity is communicated with the plurality of accommodation cavities through the liquid inlet channel to supply liquid to the accommodation cavities, both the plurality of accommodation cavities and the plurality of exhaust holes penetrate through both sides of the detection body, and the plurality of accommodation cavities and the plurality of exhaust holes are in one-to-one correspondence and communication; the first sealing sheet is located on the first side of the detection body, the second sealing sheet is located on the second side of the detection body, and the first sealing sheet and the second sealing sheet cooperate to seal the accommodation cavities; the third sealing sheet is attached to the second side of the detection body, and the first sealing sheet and the third sealing sheet cooperate to seal the exhaust holes.

[0009] Preferably, a liquid inlet channel is recessed and extended from the second side of the detection plate to the first side of the detection plate, and the second sealing sheet is further used to seal the liquid inlet channel; the liquid inlet channel includes a main channel, a connecting channel and a plurality of branch channels which are communicated; the sample injection cavity is communicated with the main channel through the connecting channel, the first ends of the plurality of branch channels are in one-to-one correspondence and communication with the plurality of accommodation cavities, and the second ends of the plurality of branch channels are all communicated with the main channel.

[0010] Preferably, the cross-section of the total channel is concave-shaped, and the openings of the total channels in the two detection units are arranged opposite to each other; each detection unit is provided with two rows of accommodation cavities, which are respectively arranged on both sides of the total channel, and each row of accommodation cavities includes a plurality of accommodation cavities arranged at intervals along the length direction of the detection body.

[0011] Preferably, it further includes a sealing plug; the sample injection cavity penetrates through both sides of the detection body; the sealing plug passes through the first sealing sheet and covers the first side of the sample injection cavity to cooperate with the second sealing sheet to seal the sample injection cavity.

[0012] Preferably, the second sealing sheet is in a shape of a Chinese character 'hui'.

[0013] Preferably, a plurality of exhaust channels are recessed on the first side of the detection plate extending towards the second side of the detection plate, and the first sealing sheet is also used to seal the exhaust channels; the plurality of exhaust channels, the plurality of accommodation cavities and the plurality of exhaust holes are arranged in one-to-one correspondence; both ends of the exhaust channel communicate with the exhaust hole and the accommodation cavity respectively.

[0014] Preferably, the thickness of the first sealing sheet and the second sealing sheet is 35 - 40 μm.

[0015] Preferably, both between the first sealing sheet and the detection body and between the second sealing sheet and the detection body are connected by heat sealing.

[0016] Preferably, it further includes a locking plate; a clamping groove is recessed on the second side of the detection plate, the clamping groove corresponds to the third sealing sheet, and the locking plate is buckled on the clamping groove of the detection plate through a sealing film to press the third sealing sheet.

[0017] Preferably, the detection plate is in a rectangular structure; the accommodation cavity is in a rectangular structure.

[0018] (III) Beneficial Effects

[0019] The beneficial effects of the present utility model are as follows:

[0020] For the microfluidic chip of the present utility model, since the detection plate includes a detection body and two detection units formed on the detection body, two different samples can be injected into the two detection units, thus improving the detection efficiency. At the same time, since the first sealing sheet and the second sealing sheet are respectively located on both sides of the accommodation cavity for sealing, it is convenient for the subsequent amplification device to heat both surfaces of the accommodation cavity simultaneously, shortening the PCR reaction time, thereby shortening the nucleic acid detection time, and making the interior of the chamber heat evenly and balancing the temperature difference inside the liquid, improving the accuracy of the detection result. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 is an exploded schematic view of the microfluidic chip of the present utility model;

[0022] Figure 2 is Figure 1Top view of the detection plate;

[0023] Figure 3 is Figure 1 Bottom view of the detection plate.

[0024]

Explanation of the reference numerals of the drawings

[0025] 1: Detection plate; 11: Detection body; 12: Detection unit; 121: Injection cavity; 122: Liquid inlet channel; 1221: Main channel; 1222: Connection channel; 1223: Branch channel; 123: Accommodation cavity; 124: Exhaust hole; 125: Exhaust channel; 13: Clamping groove;

[0026] 2: First sealing piece;

[0027] 3: Second sealing piece;

[0028] 4: Third sealing piece;

[0029] 5: Sealing plug;

[0030] 6: Locking plate;

[0031] 7: Sealing film. Detailed implementation manners

[0032] For better explaining the present utility model and facilitating understanding, the present utility model will be described in detail below with reference to the drawings through specific implementation manners.

[0033] As Figure 1 shown, this embodiment provides a microfluidic chip, which includes a detection plate 1, a first sealing piece 2, a second sealing piece 3 and two third sealing pieces 4.

[0034] As Figure 2 and Figure 3 shown, the detection plate 1 includes a detection body 11 and two detection units 12. The two detection units 12 are provided on the detection body 11 and are mirror-symmetrically arranged. The detection unit 12 includes an injection cavity 121, a liquid inlet channel 122, a plurality of accommodation cavities 123 and a plurality of exhaust holes 124. The injection cavity 121 is communicated with the plurality of accommodation cavities 123 through the liquid inlet channel 122 to supply liquid to the accommodation cavities 123. The plurality of accommodation cavities 123 and the plurality of exhaust holes 124 both penetrate through two sides of the detection body 11, and the plurality of accommodation cavities 123 and the plurality of exhaust holes 124 are in one-to-one correspondence and communication. The first sealing piece 2 is located on the first side of the detection body 11, the second sealing piece 3 is located on the second side of the detection body 11, the first sealing piece 2 and the second sealing piece 3 cooperate to seal the accommodation cavity 123, the third sealing piece 4 is attached to the second side of the detection body 11, and the first sealing piece 2 and the third sealing piece 4 cooperate to seal the exhaust hole 124. Among them, the detection plate 1 is a rectangular structure, and the accommodation cavity 123 is a rectangular structure.

[0035] In this embodiment, since the detection plate 1 includes two detection units 12 formed on the detection body 11, two different samples can be injected into the two detection units 12 for simultaneous detection, thus improving the detection efficiency. At the same time, since the accommodation cavity 123 penetrates through the detection body 11 and the first sealing piece 2 and the second sealing piece 3 are respectively located on both sides of the accommodation cavity 123 for sealing, it is convenient for the subsequent amplification device to heat both surfaces of the accommodation cavity 123 simultaneously, shortening the PCR reaction time, further shortening the nucleic acid detection time, and making the interior of the cavity heated evenly and balancing the temperature difference inside the liquid, thereby improving the accuracy of the detection result.

[0036] In this embodiment, the thickness of the first sealing piece 2 and the second sealing piece 3 is 35 - 40 μm, so that the first sealing piece 2 and the second sealing piece 3 have fast heat transfer on the premise of not being easily damaged. The third sealing piece 4 is made of a multi-layer composite material, preferably a non-water-absorbing material, which avoids the third sealing piece 4 sucking the liquid from the exhaust hole 124 during the amplification reaction and generating bubbles in the accommodation cavity 123, thereby affecting the detection accuracy.

[0037] To improve the reliability of the connection between the first sealing piece 2 and the second sealing piece 3 and the detection body 11, both between the first sealing piece 2 and the detection body 11 and between the second sealing piece 3 and the detection body 11 are connected by heat sealing to prevent the reagent or sample from overflowing during the thermal reaction process.

[0038] As Figure 2 shown, a liquid inlet channel 122 is recessed on the second side of the detection plate 1 extending towards the first side of the detection plate 1, and the second sealing piece 3 is also used to seal the liquid inlet channel 122. The liquid inlet channel 122 includes a connected main channel 1221, a connecting channel 1222, and a plurality of branch channels 1223. The sample injection cavity 121 is connected to the main channel 1221 through the connecting channel 1222. The first ends of the plurality of branch channels 1223 are respectively connected to the plurality of accommodation cavities 123 in one-to-one correspondence, and the second ends of the plurality of branch channels 1223 are all connected to the main channel 1221. Among them, the cross-section of the main channel 1221 is concave-shaped, and the openings of the main channels 1221 in the two detection units 12 are arranged oppositely. Each detection unit 12 is provided with two rows of accommodation cavities 123, and the two rows of accommodation cavities 123 are respectively arranged on both sides of the main channel 1221. Each row of accommodation cavities 123 includes a plurality of accommodation cavities 123 arranged at intervals along the length direction of the detection body 11.

[0039] As Figure 1 shown, for the convenience of sealing the sample injection cavity 121, the microfluidic chip further includes a sealing plug 5. The sample injection cavity 121 penetrates through both sides of the detection body 11, and the sealing plug 5 passes through the first sealing piece 2 and covers the first side of the sample injection cavity 121 to cooperate with the second sealing piece 3 to seal the sample injection cavity 121. In the actual application process, the second sealing piece 3 has a square frame structure.

[0040] As shown Figure 3 in the figure, a plurality of exhaust channels 125 are recessed and extended from the first side of the detection plate 1 to the second side of the detection plate 1, and the first sealing sheet 2 is also used to seal the exhaust channels 125. Among them, the plurality of exhaust channels 125, the plurality of accommodation cavities 123 and the plurality of exhaust holes 124 are arranged in one-to-one correspondence, and both ends of the exhaust channel 125 communicate with the exhaust hole 124 and the accommodation cavity 123 respectively.

[0041] To prevent the third sealing sheet 4 from being pushed open during the amplification process, the microfluidic chip further includes a locking plate 6. A clamping groove 13 is recessed on the second side of the detection plate 1. The clamping groove 13 is arranged corresponding to the third sealing sheet 4. The locking plate 6 is buckled on the clamping groove 13 of the detection plate 1 through a sealing film to press the third sealing sheet 4.

[0042] It should be noted that in this embodiment, two sealing plugs 5, two locking plates 6, two sealing films 7 and two third sealing sheets 4 are provided to respectively correspond to the two detection units 12.

[0043] In the description of the present invention, it should be understood that the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the present invention, the meaning of "a plurality of" is two or more unless otherwise specifically defined.

[0044] In the present invention, unless otherwise clearly defined and limited, the terms such as "installation", "connection", "connection", "fixation" and other terms should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection or integrated; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium; it can be the internal communication of two components or the interaction relationship between two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0045] In the present invention, unless otherwise clearly defined and limited, the first feature being "on" or "under" the second feature can be that the first and second features are in direct contact, or the first and second features are indirectly in contact through an intermediate medium. Moreover, the first feature being "above", "over" and "on" the second feature can be that the first feature is directly above or obliquely above the second feature, or only indicates that the first feature is at a higher horizontal height than the second feature. The first feature being "under", "beneath" and "under" the second feature can be that the first feature is directly below or obliquely below the second feature, or only indicates that the first feature is at a lower horizontal height than the second feature.

[0046] In the description of this specification, the descriptions of terms such as "one embodiment", "some embodiments", "embodiment", "example", "specific example" or "some examples" mean that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present utility model. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, without contradiction, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples.

[0047] Although the embodiments of the present utility model have been shown and described above, it can be understood that the above embodiments are exemplary and should not be construed as limiting the present utility model. Those of ordinary skill in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present utility model.

Claims

1. A microfluidic chip, characterized in that: It comprises a detection plate (1), a first sealing sheet (2), a second sealing sheet (3) and two third sealing sheets (4); The detection board (1) comprises a detection body (11) and two detection units (12) provided on the detection body (11), wherein the two detection units (12) are arranged in a mirror-symmetrical manner; The detection unit (12) comprises a sample injection chamber (121), a liquid inlet channel (122), a plurality of accommodating chambers (123) and a plurality of exhaust holes (124); the sample injection chamber (121) is connected to the plurality of accommodating chambers (123) through the liquid inlet channel (122) to supply liquid to the accommodating chambers (123); the plurality of accommodating chambers (123) and the plurality of exhaust holes (124) both penetrate two sides of the detection body (11); and the plurality of accommodating chambers (123) and the plurality of exhaust holes (124) are connected in a one-to-one correspondence; The first sealing sheet (2) is located on a first side of the detection body (11), and the second sealing sheet (3) is located on a second side of the detection body (11), and the first sealing sheet (2) and the second sealing sheet (3) cooperate to seal the accommodating cavity (123); The third sealing sheet (4) is attached to the second side of the detection body (11), and the first sealing sheet (2) and the third sealing sheet (4) cooperate to seal the exhaust hole (124).

2. The microfluidic chip according to claim 1, characterized in that: The second side of the detection plate (1) is extended toward the first side of the detection plate (1) and is concavely provided with the liquid inlet channel (122), and the second sealing sheet (3) is also used to seal the liquid inlet channel (122); The liquid inlet channel (122) comprises a main channel (1221), a connecting channel (1222) and a plurality of branch channels (1223) that are connected to each other; The injection cavity (121) is connected to the main channel (1221) via a connecting channel (1222); the first ends of the plurality of branch channels (1223) are connected to the plurality of accommodating cavities (123) in a one-to-one correspondence; and the second ends of the plurality of branch channels (1223) are all connected to the main channel (1221).

3. The microfluidic chip according to claim 2, characterized in that: The cross section of the main channel (1221) is concave-shaped, and the openings of the main channels (1221) in the two detection units (12) are arranged opposite to each other; Each of the detection units (12) is provided with two rows of accommodating cavities (123), the two rows of accommodating cavities (123) being respectively arranged on both sides of the main channel (1221), and each row of the accommodating cavities (123) comprising a plurality of accommodating cavities (123) arranged at intervals along the length direction of the detection body (11).

4. The microfluidic chip according to claim 3, characterized in that: Also includes a sealing plug (5); The sample injection cavity (121) runs through both sides of the detection body (11); The sealing plug (5) passes through the first sealing sheet (2) and is covered on the first side of the injection cavity (121) to cooperate with the second sealing sheet (3) to seal the injection cavity (121).

5. The microfluidic chip according to claim 4, characterized in that: The second sealing sheet (3) is of a U-shaped structure.

6. The microfluidic chip according to claim 1, characterized in that: A plurality of exhaust channels (125) are recessed and extend from the first side of the detection plate (1) toward the second side of the detection plate (1), and the first sealing sheet (2) is also used to seal the exhaust channels (125); The plurality of exhaust channels (125), the plurality of accommodating chambers (123) and the plurality of exhaust holes (124) are arranged in a one-to-one correspondence; the two ends of the exhaust channels (125) are respectively connected to the exhaust holes (124) and the accommodating chambers (123).

7. The microfluidic chip according to claim 1, characterized in that: The thickness of the first sealing sheet (2) and the second sealing sheet (3) is 35-40 μm.

8. The microfluidic chip according to claim 1, characterized in that: The first sealing sheet (2) and the detection body (11), as well as the second sealing sheet (3) and the detection body (11) are connected by heat sealing.

9. The microfluidic chip according to claim 1, characterized in that: Also includes a locking plate (6); A snap-fit ​​groove (13) is recessed on the second side of the detection plate (1), the snap-fit ​​groove (13) being arranged corresponding to the third sealing sheet (4), and the locking plate (6) is buckled on the snap-fit ​​groove (13) of the detection plate (1) via a sealing rubber sheet (7) to press the third sealing sheet (4).

10. The microfluidic chip according to claim 1, characterized in that: The detection board (1) is a rectangular structure; The accommodating cavity (123) is a rectangular structure.

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