Ultrafiltration centrifuge tube

Abstract

The utility model belongs to the technical field of centrifuge tubes, and provides an ultrafiltration centrifuge tube, which comprises: an outer tube, the bottom of the outer tube is provided with a collection cavity for collecting filtered substances, and the top end of the outer tube is provided with a loading port; the inner tube is used for containing a sample to be centrifugally filtered, and the inner tube can extend into the outer tube from the loading opening or move out of the outer tube; a limiting structure for limiting the depth of the inner pipe extending into the outer pipe is arranged on the inner wall of the outer pipe; according to the invention, the inner tube is inverted and arranged in the outer tube, the inner tube is blocked by the limiting structure, and the residual filter sample in the inner tube can directly enter the collecting cavity of the other outer tube, so that the transfer and recovery of the filter sample are completed, and the transfer and recovery of the filter sample can be carried out without adopting a pipette; the filtered sample in the inner tube is directly transferred and recycled into the other outer tube, so that the transferring and recycling operation is fast, and the recovery rate of the filtered sample is high.

Description

Technical Field

[0001] This application relates to the technical field of centrifuge tubes, and particularly to an ultrafiltration centrifuge tube. Background Art

[0002] Centrifugation technology is one of the most commonly used methods for separating proteins, enzymes, nucleic acids, and cell subcomponents, and is also a common method for separation, purification, or clarification in biochemical laboratories. With the in-depth development of life science research, the demand for high-purity, rapid separation, and concentration of biological samples is increasing. Although traditional centrifuge tubes can achieve preliminary separation of samples, they have limitations in removing small-molecule impurities and achieving efficient filtration.

[0003] In related technologies for filtering trace samples, after the sample is filtered in the centrifuge tube, it is usually necessary to transfer and recover the concentrated sample with a pipette. However, when using a pipette for sample transfer and recovery, there are problems such as long operation time, puncturing of the filter membrane, and low recovery rate. Utility Model Content

[0004] In view of the above-mentioned disadvantages of the prior art, the purpose of this application is to provide an ultrafiltration centrifuge tube for solving the problem of low recovery rate when using a pipette for sample transfer and recovery in filtering trace samples.

[0005] To achieve the above object and other related objects, this application provides an ultrafiltration centrifuge tube, including:

[0006] An outer tube, the bottom of the outer tube has a collection cavity for collecting filtrate, and the top of the outer tube is provided with a loading port;

[0007] An inner tube for accommodating the sample to be centrifuged and filtered, and the inner tube can extend into the outer tube from the loading port or be removed from the outer tube;

[0008] The inner wall of the outer tube is provided with a limiting structure for restricting the depth of the inner tube extending into the outer tube.

[0009] Optionally, the first end of the inner tube extends into the outer tube, and the limiting structure abuts against the end of the first end of the inner tube.

[0010] Optionally, the limiting structure includes protrusions radially distributed along the inner wall of the outer tube, and the protrusions are continuous or discontinuous.

[0011] Optionally, the inner tube includes an open section and a filtering section connected to each other. The open section has an opening for the sample to enter. Opposite sides of the filtering section are provided with mounting surfaces, and filtering holes and a filter membrane covering the filtering holes are provided on the mounting surfaces. A collection groove for accommodating the filtered sample is provided at the bottom of the filtering section.

[0012] Optionally, when the inner tube is inserted into the outer tube, at least a part of the open section is exposed from the loading opening, and the outer diameter of the open section is smaller than the inner diameter of the outer tube.

[0013] Optionally, a protective housing is sleeved outside the filtering section. When the inner tube is inserted into the outer tube, the end face of the protective housing abuts against the limiting structure.

[0014] Optionally, the outer diameters of both the open section and the protective housing are larger than the inner diameter of the limiting ring formed by the protrusions.

[0015] Optionally, the inner wall of the protective housing is in close contact with the filter membrane, and diversion grooves are formed on the inner wall of the protective housing for guiding the filtered matter into the outer tube.

[0016] Optionally, the mounting surface is an inclined surface, and the two mounting surfaces gradually approach each other along the insertion direction of the inner tube.

[0017] Optionally, flat-shaped grasping portions are provided on both sides of the inner tube. After the inner tube is inserted into the outer tube, a clearance notch is formed between the grasping portions and the inner wall of the outer tube.

[0018] In the ultrafiltration centrifuge tube provided by the present application, first, the inner tube is inserted into the outer tube from the loading opening, then the sample is added into the inner tube for centrifugal filtration, and the filtered matter is collected in the collection cavity at the bottom of the outer tube. The remaining filtered sample in the inner tube is taken. Another outer tube is taken, and the inner tube is inverted and inserted into the other outer tube. Blocked by the limiting structure, the remaining filtered sample in the inner tube can directly enter the collection cavity of the other outer tube, completing the transfer and recovery of the filtered sample. It is not necessary to use a pipette to transfer and recover the filtered sample. The filtered sample in the inner tube can be directly transferred and recovered into another outer tube, which not only has a fast transfer and recovery operation but also a high recovery rate of the filtered sample. Description of the Drawings

[0019] Figure 1 It is a schematic structural view of the ultrafiltration centrifuge tube shown in the first embodiment of the present application;

[0020] Figure 2 It is a cross-sectional view of the ultrafiltration centrifuge tube shown in the first embodiment of the present application;

[0021] Figure 3 It is a schematic structural view of the inner tube and the protective housing shown in the first embodiment of the present application;

[0022] Figure 4 It is a schematic structural view of the ultrafiltration centrifuge tube shown in the second embodiment of the present application;

[0023] Figure 5 It is a schematic structural view of the inner tube and the protective housing shown in the second embodiment of the present application.

[0024] Description of Part Numbers

[0025] 1 - Outer tube; 2 - Inner tube; 3 - Tube cap; 4 - First sealing part; 5 - Second sealing part; 6 - Connector; 7 - Collection cavity; 8 - Limiting structure; 9 - Open section; 10 - Filter section; 11 - Open end; 12 - Collection groove; 13 - Protective housing; 14 - Mounting surface; 15 - Filter membrane; 16 - Flow guiding groove; 17 - Gripping part. Specific Embodiment

[0026] The following specific embodiments illustrate the implementation manners of the present application. Those skilled in the art can easily understand other advantages and effects of the present application from the content disclosed in this specification.

[0027] It should be noted that the structures, ratios, sizes, etc. shown in the drawings of this specification are only used to cooperate with the content disclosed in the specification for those skilled in the art to understand and read, and are not used to limit the limiting conditions under which the present application can be implemented. Therefore, they do not have a technical essence. Any modification of the structure, change of the proportional relationship, or adjustment of the size, without affecting the effects that the present application can produce and the purposes that can be achieved, should still fall within the scope covered by the technical content disclosed in the present application. At the same time, the terms such as "front", "rear", "left", "right", "middle", and "one" cited in this specification are only for the convenience of clear narration and are not used to limit the scope under which the present application can be implemented. The change or adjustment of their relative relationships, without substantial change in the technical content, should also be regarded as the scope under which the present application can be implemented.

[0028] It should be noted that for the concentration and purification of proteins, the traditional technical means of centrifuge tubes is to obtain samples through filtration and centrifugation to dry, dilute them with buffer or bovine serum albumin, and then aspirate the samples from the centrifuge tubes with a pipette. However, the recovery transfer of samples with a pipette is cumbersome, time-consuming, and has a low recovery rate. For samples with a low recovery rate, there will be a large error in the determination. Therefore, although traditional centrifuge tubes can achieve the preliminary separation of samples, they still have limitations in removing small molecule impurities and achieving efficient filtration. In related technologies, some centrifuge tubes use a horizontally designed filter membrane to bind analytes, and then use a suitable eluent to elute the analytes. This method is cumbersome, and there is a problem of low accuracy in the determination of substances with low content. Moreover, the horizontally designed filter membrane has a small filtration area and is easy to clog. Some centrifuge tubes still fix the filter membrane inside the centrifuge tube, and it is very easy to pierce the filter membrane when adding samples with a pipette, causing damage to the filter membrane.

[0029] Example 1: Please refer to Figures 1 to 3 , a ultrafiltration centrifuge tube, comprising:

[0030] An outer tube 1, the bottom of the outer tube 1 has a collection cavity 7 for collecting filtrates, and an inlet is provided at the top of the outer tube 1;

[0031] An inner tube 2 for accommodating a sample to be centrifugally filtered, and the inner tube 2 can extend into the outer tube 1 from the loading port or be removed from the outer tube 1.

[0032] A limiting structure 8 for limiting the depth of the inner tube 2 extending into the outer tube 1 is provided on the inner wall of the outer tube 1, and when the inner tube 2 is inserted into the outer tube 1, at least a part of the inner tube 2 is exposed from the loading port.

[0033] In the ultrafiltration centrifuge tube provided by the present application, first, the inner tube 2 is inserted into the outer tube 1 from the loading port. The inner tube 2 is blocked by the limiting structure 8. Preferably, at least a part of the inner tube 2 is exposed from the loading port. Then, the sample is added into the inner tube 2 for centrifugal filtration. The filtrate is collected into the collection chamber 7 at the bottom of the outer tube 1. For the remaining filtered sample in the inner tube 2, take another outer tube 1, invert the inner tube 2 and insert it into the other outer tube 1. The inner tube 2 is blocked by the limiting structure 8, and the remaining filtered sample in the inner tube 2 can directly enter the collection chamber 7 of the other outer tube 1, completing the transfer and recovery of the filtered sample. It is not necessary to use a pipette for the transfer and recovery of the filtered sample, avoiding damage to the filter membrane 15 by the pipette. By inverting the inner tube 2 and inserting it into another outer tube 1, the filtered sample in the inner tube 2 can be directly transferred and recovered into the other outer tube 1. Not only is the transfer and recovery operation fast, but also the recovery rate of the filtered sample is high.

[0034] In addition, when there is remaining filtered sample in the inner tube 2, the operator can also directly use a pipette to pipette out the filtered sample in the inner tube 2.

[0035] In this embodiment, during centrifugal filtration, the first end of the inner tube 2 extends into the outer tube 1, and the second end is exposed from the outer tube 1. The limiting structure 8 abuts against the end of the first end of the inner tube 2. After filtration is completed, the inner tube 2 is inverted and inserted into the outer tube 1. The second end of the inner tube 2 extends into the outer tube 1, and the first end is exposed from the outer tube 1. The limiting structure 8 abuts against the end of the second end of the inner tube 2. Through the part of the inner tube 2 exposed from the outer tube 1, it is convenient to remove the inner tube 2 from the outer tube 1.

[0036] In this embodiment, please refer to Figure 2 , the limiting structure 8 includes protrusions radially distributed along the inner wall of the outer tube 1, blocking in the loading direction of the inner tube 2. The protrusions are continuous or discontinuous and enclose to form a limiting ring.

[0037] In some embodiments, the inner tube 2 includes an open section 9 and a filtering section 10 connected to each other. The open section 9 has an opening 11 for the sample to enter. Mounting surfaces 14 are oppositely arranged on both sides of the filtering section 10. Filtering holes and a filtering membrane 15 covering the filtering holes are provided on the mounting surfaces 14. A collection groove 12 for accommodating the filtered sample is provided at the bottom of the filtering section 10. Specifically, the filtering membranes 15 are symmetrically arranged. By symmetrically arranging the filtering membranes 15 on both sides of the filtering section 10 of the inner tube 2, more membrane area can be provided, achieving a better filtering effect.

[0038] In the above embodiment, when the inner tube 2 is inserted into the outer tube 1, at least a part of the open section 9 is exposed at the insertion opening. The outer diameter of the open section 9 is smaller than the inner diameter of the outer tube 1, and the outer diameter of the opening 11 of the open section 9 is larger than the inner diameter of the limiting ring formed by the protrusion. When the inner tube 2 is inserted into the outer tube 1 in an inverted manner, the opening 11 of the open section 9 is blocked by the blocking structure, realizing the inversion of the inner tube 2 inside the outer tube 1.

[0039] In this embodiment, as Figure 2 and Figure 3 shown, a protective housing 13 is sleeved outside the filtering section 10. When the inner tube 2 is inserted into the outer tube 1, the end face of the protective housing 13 abuts against the limiting structure 8. Specifically, the outer diameter of the protective housing 13 is larger than the inner diameter of the limiting ring surrounded by the protrusions. By surrounding the filtering section 10 with the protective housing 13, the contact between the outside of the inner tube 2 and the inner wall of the outer tube 1 is increased, reducing the risk of the inner tube 2 breaking during centrifugation.

[0040] Specifically, the inner wall of the protective housing 13 is in fitting contact with the filtering membrane 15. Flow guiding grooves 16 are provided on the inner wall of the protective housing 13 for guiding the impurities filtered out by the filtering membrane 15 into the outer tube 1. Through the arrangement of the flow guiding grooves 16, there is no need to reserve a gap for liquid to flow out between the inner tube 2 and the outer tube 1. The outside of the inner tube 2 can be in fitting contact with the inner wall of the outer tube 1. The centrifugal force received by the inner tube 2 can be dispersed to the outer tube 1 to the maximum extent, further reducing the risk of the inner tube 2 breaking. At the same time, the openings of the flow guiding grooves 16 face the collection cavity 7 of the outer tube 1, and the impurities filtered out by the filtering membrane 15 can quickly flow into the collection cavity 7, improving the liquid flow rate and saving the filtering time.

[0041] In some embodiments, please refer to Figure 2 , the mounting surfaces 14 are inclined surfaces, and the two mounting surfaces 14 gradually approach along the insertion direction of the inner tube 2. Through the inclined arrangement of the mounting surfaces 14, the filtering membrane 15 is covered in an approximately vertical state, which can reduce blockage during the tangential flow filtration of the sample liquid, increase the effective filtration area of the filtering membrane 15. At the same time, when using a pipette to transfer the filtered sample, it can also avoid piercing the filtering membrane 15 and causing damage to the filtering membrane 15.

[0042] In this embodiment, flat gripping portions 17 are provided on both sides of the inner tube 2. The gripping portions 17 extend from one end of the protective outer shell 13 away from the opening 11 to the opening 11 and form a flat structure on the outer wall of the inner tube 2. After the inner tube 2 is installed in the outer tube 1, a clearance gap is formed between the gripping portions 17 and the inner wall of the outer tube 1. The provision of the gripping portions 17 increases the stability of the inner tube 2 when it is clamped with tweezers or grasped by hand in a wet condition, making it easier to take and place the inner tube 2.

[0043] In the above embodiment, the ultrafiltration centrifuge tube also includes a tube cover 3 for sealing the inner tube 2. The tube cover 3 has a first sealing portion 4 and a second sealing portion 5 connected to each other on the side facing the inner tube 2. The first sealing portion 4 matches the open port 11 of the inner tube 2, and the second sealing portion 5 matches the loading port of the outer tube 1. During the centrifugation process, the tube cover 3 is covered on the open port 11, and the first sealing portion 4 extends into the open port 11 to seal the inner tube 2 to prevent the sample in the inner tube 2 from running out under the action of centrifugation. After centrifugal filtration, another outer tube 1 is taken, and the inner tube 2 is inverted and loaded into the outer tube 1. Then, the tube cover 3 is covered on the loading port, and the first sealing portion 4 and the second sealing portion 5 are simultaneously extended into the loading port. The second sealing portion 5 is in contact with the inner wall of the outer tube 1 to seal the outer tube 1 to prevent the concentrated sample in the outer tube 1 from running out when the outer tube 1 is moved.

[0044] Specifically, the tube cover 3 is connected to the outer tube 1 through the connector 6. The connector 6 is a flexible structure. The tube cover 3 is connected to the outer tube 1 as a whole through the connector 6, so as to avoid the tube cover 3 from being lost and facilitate operation.

[0045] Embodiment 2: The technical solution of Embodiment 2 is basically the same as that of Embodiment 1, except that:

[0046] like Figure 4 and Figure 5 As shown, the gripping portion 17 on the inner tube 2 does not extend to the opening 11 . The opening 11 is a circular structure. The first sealing portion 4 is also a circular structure and matches the opening 11 .

[0047] In summary, in the ultrafiltration centrifuge tube provided in this application, first, the inner tube 2 is inserted into the outer tube 1 from the loading port. The inner tube 2 is blocked by the limiting structure 8. Preferably, at least a part of the inner tube 2 is exposed from the loading port. Then, a sample is added to the inner tube 2 for centrifugal filtration. The filtrate is collected in the collection chamber 7 at the bottom of the outer tube 1. The remaining filtered sample in the inner tube 2 is taken. Another outer tube 1 is taken, and the inner tube 2 is inverted and inserted into another outer tube 1. The inner tube 2 is blocked by the limiting structure 8. The remaining filtered sample in the inner tube 2 can directly enter the collection chamber 7 of another outer tube 1, completing the transfer and recovery of the filtered sample. It is not necessary to use a pipette for the transfer and recovery of the filtered sample, avoiding damage to the filter membrane 15 by the pipette. By inverting the inner tube 2 and inserting it into another outer tube 1, the filtered sample in the inner tube 2 can be directly transferred and recovered into another outer tube 1. Not only is the transfer and recovery operation fast, but also the recovery rate of the filtered sample is high.

[0048] The above embodiments merely exemplarily illustrate the principles and effects of this application, rather than limiting this application. Any person familiar with this technology can modify or change the above embodiments without departing from the spirit and scope of this application. Therefore, all equivalent modifications or changes completed by those with ordinary knowledge in the technical field without departing from the spirit and technical ideas disclosed in this application should still be covered by the claims of this application.

Claims

1. An ultrafiltration centrifuge tube, characterized in that: include: An outer tube, wherein the bottom of the outer tube has a collecting cavity for collecting filtrate, and the top of the outer tube is provided with a loading port; An inner tube, used for containing a sample to be centrifugally filtered, wherein the inner tube can be extended into the outer tube from the loading port or removed from the outer tube; The inner wall of the outer tube is provided with a limiting structure for limiting the depth of the inner tube extending into the outer tube.

2. The ultrafiltration centrifuge tube according to claim 1, characterized in that: The first end of the inner tube extends into the outer tube, and the limiting structure abuts against the end of the first end of the inner tube.

3. The ultrafiltration centrifuge tube according to claim 2, characterized in that: The limiting structure includes protrusions radially distributed along the inner wall of the outer tube, and the protrusions are continuous or discontinuous.

4. The ultrafiltration centrifuge tube according to claim 3, characterized in that: The inner tube includes an open section and a filtering section that are connected to each other. The open section has an opening for samples to enter. Mounting surfaces are arranged opposite to each other on both sides of the filtering section. Filter holes and a filter membrane covering the filter holes are arranged on the mounting surfaces. A collection tank for accommodating filtered samples is arranged at the bottom of the filtering section.

5. The ultrafiltration centrifuge tube according to claim 4, characterized in that: When the inner tube is inserted into the outer tube, the open section at least partially exposes the insertion port, and the outer diameter of the open section is smaller than the inner diameter of the outer tube.

6. The ultrafiltration centrifuge tube according to claim 4, characterized in that: The outer side of the filter section is sleeved with a protective shell. When the inner tube is installed into the outer tube, the end surface of the protective shell abuts against the limiting structure.

7. The ultrafiltration centrifuge tube according to claim 6, characterized in that: The outer diameters of the open section and the protective shell are both larger than the inner diameter of the limiting ring formed by the protrusion.

8. The ultrafiltration centrifuge tube according to claim 6, characterized in that: The inner wall of the protective shell is in close contact with the filter membrane, and a guide groove is provided on the inner wall of the protective shell for guiding the filtrate into the outer tube.

9. The ultrafiltration centrifuge tube according to claim 4, characterized in that: The installation surface is an inclined surface, and the two installation surfaces gradually approach each other along the installation direction of the inner tube.

10. The ultrafiltration centrifuge tube according to claim 9, characterized in that: Flat gripping parts are arranged on both sides of the inner tube. After the inner tube is installed into the outer tube, a clearance gap is formed between the gripping parts and the inner wall of the outer tube.

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