Fluorescent quantitative detection module with multi-channel light insulation function

Abstract

The fluorescent quantitative detection module comprises a light insulation bottom shell and a light insulation upper cover arranged on the light insulation bottom shell, at least two fluorescent quantitative detection channels are arranged on the light insulation bottom shell, and detection light paths of the at least two fluorescent quantitative detection channels are linearly and tightly arranged. A light isolation wall is arranged between every two adjacent fluorescent quantitative detection channels. On the basis of ensuring the accuracy of a single fluorescent quantitative detection channel, a plurality of fluorescent quantitative detection channels are additionally arranged, so that the fluorescent quantitative detection efficiency can be improved, single-channel operation and multi-channel operation can be realized, light is isolated among the fluorescent quantitative detection channels through the light isolating walls during multi-channel operation, and the fluorescent quantitative detection efficiency is improved. And each channel is ensured not to be interfered during operation, so that the accuracy of the detection result of each fluorescent quantitative detection channel is ensured.

Description

Technical Field

[0001] The utility model relates to the technical field of molecular quantitative detection, and particularly relates to a fluorescence quantitative detection module with multi-channel light isolation. Background Art

[0002] In modern molecular quantitative analysis research, fluorescence quantitative detection technology is widely used in the fields of disease diagnosis, genetic research, etc. due to its high sensitivity and specificity. However, as Figure 1 shown, the traditional single-channel fluorescence detection system includes a two-channel light isolation wall 10. A single-channel for fluorescence detection is formed between the two-channel light isolation walls 10. An LED emission optical path 11 and a solution excitation optical path 12 are arranged in the channel. Among them, the LED emission optical path 11 includes an LED light source 13, an input filter 14, and an input sensor 15 arranged in sequence. The solution excitation optical path 12 includes an output filter 16 and an output sensor 17 arranged in sequence. A test tube placement position 18 is located between the input sensor 15 and the output filter 16, and the optical path forms a certain angle α. The traditional single-channel fluorescence detection system has problems such as low detection efficiency and large volume. In addition, the application of multi-channel quantitative detection technology also faces the problem of mutual interference between fluorescence channels, which directly affects the accuracy of the detection results. Summary of the Utility Model

[0003] The main purpose of the utility model is to propose a fluorescence quantitative detection module with multi-channel light isolation, aiming to improve the efficiency and accuracy of fluorescence detection to be applicable to molecular biology detection technologies such as real-time quantitative detection.

[0004] To achieve the above purpose, the utility model provides a fluorescence quantitative detection module with multi-channel light isolation, including: a light isolation bottom case and a light isolation upper cover arranged on the light isolation bottom case. At least two fluorescence quantitative detection channels are arranged on the light isolation bottom case, and the detection optical paths of the at least two fluorescence quantitative detection channels are linearly and closely arranged, and there is a light isolation wall between adjacent fluorescence quantitative detection channels.

[0005] A further technical solution of the utility model is that an LED emission optical path and a solution excitation optical path are arranged in each fluorescence quantitative detection channel. A test tube placement position is between the LED emission optical path and the solution excitation optical path, and an angle α is formed between the LED emission optical path and the solution excitation optical path, where 30° < α < 180°.

[0006] A further technical solution of the utility model is that the angle α between the LED emission optical path and the solution excitation optical path is 120°.

[0007] A further technical solution of the present utility model is that the horizontal height of the opening of the light-shielding bottom shell is higher than the horizontal height of the components in the LED emission optical path and the components in the solution excitation optical path.

[0008] A further technical solution of the present utility model is that a rabbet is formed by upward protrusion at the opening of the light-shielding bottom shell, and a groove corresponding to the rabbet is provided on the light-shielding upper cover.

[0009] A further technical solution of the present utility model is that the components in the LED emission optical path include an LED light source, an input filter, and an input sensor arranged in sequence from front to back, and the solution excitation optical path includes an output filter and an output sensor arranged in sequence from front to back.

[0010] The beneficial effect of the present utility model having a multi-channel light-shielding fluorescence quantitative detection module is as follows:

[0011] Through the above technical solution, on the basis of ensuring the accuracy of a single fluorescence quantitative detection channel, the present utility model increases multiple fluorescence quantitative detection channels, which can improve the fluorescence quantitative detection efficiency. It can operate either in a single channel or in multiple channels. When operating in multiple channels, the light between each fluorescence quantitative detection channel is shielded by the light-shielding wall, ensuring that each channel operates without interference, and thus ensuring the accuracy of the detection results of each fluorescence quantitative detection channel. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present utility model. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on the structures shown in these drawings.

[0013] Figure 1 It is a schematic diagram of the optical path structure of a single-channel fluorescence detection system in the prior art;

[0014] Figure 2 It is a schematic structural diagram of a preferred embodiment of the multi-channel light-shielding fluorescence quantitative detection module of the present utility model without the light-shielding upper cover;

[0015] Figure 3 It is a cross-sectional view of a preferred embodiment of the multi-channel light-shielding fluorescence quantitative detection module of the present utility model;

[0016] Figure 4 It is a schematic diagram of the optical path structure of a preferred embodiment of the multi-channel light-shielding fluorescence quantitative detection module of the present utility model;

[0017] Figure 5It is a schematic diagram of the overall structure of a preferred embodiment of the fluorescence quantitative detection module with multi-channel light isolation of the present utility model.

[0018] The realization of the purpose, functional characteristics and advantages of the present utility model will be further described with reference to the embodiments and the accompanying drawings. Specific embodiments

[0019] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described with reference to the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present utility model.

[0020] It should be noted that if there are directional indications (such as up, down, left, right, front, back...) involved in the embodiments of the present utility model, the directional indications are only used to explain the relative positional relationship and movement conditions between components in a specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indications will also change accordingly.

[0021] In addition, if there are descriptions such as "first", "second", etc. involved in the embodiments of the present utility model, the descriptions of "first", "second", etc. are only for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include at least one of such features. In addition, the meaning of "and / or" appearing throughout the text includes three parallel solutions. Taking "A and / or B" as an example, it includes solution A, solution B, or a solution where A and B are satisfied simultaneously. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the fact that those of ordinary skill in the art can implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the protection scope required by the present utility model.

[0022] To solve the problem of mutual interference between channels during multi-channel fluorescence quantitative detection, the present utility model proposes a multi-channel light isolation design with a horizontal opening of the light isolation channel higher than the horizontal height of the components and with a rabbet for light isolation. Combining with the real-time fluorescence quantitative detection technology, efficient multi-channel fluorescence quantitative detection is realized.

[0023] Specifically, please refer to Figures 2 to 5, A preferred embodiment of the fluorescence quantitative detection module with multi-channel light isolation of the present utility model includes a light isolation bottom case 201 and a light isolation upper cover 202 provided on the light isolation bottom case 201. At least two fluorescence quantitative detection channels are provided on the light isolation bottom case 201, and the detection light paths of the at least two fluorescence quantitative detection channels are linearly and closely arranged, and there is a light isolation wall 203 between adjacent fluorescence quantitative detection channels.

[0024] On the basis of ensuring the accuracy of a single fluorescence quantitative detection channel, this embodiment adds multiple fluorescence quantitative detection channels, which can improve the fluorescence quantitative detection efficiency. It can operate in a single channel or in multiple channels. When operating in multiple channels, the light between each fluorescence quantitative detection channel is isolated by the light isolation wall 203 to ensure that each channel is not interfered during operation, and thus ensure the accuracy of the detection results of each fluorescence quantitative detection channel.

[0025] Further, in this embodiment, an LED emission light path 204 and a solution excitation light path 205 are provided in each fluorescence quantitative detection channel. A test tube placement position is between the LED emission light path 204 and the solution excitation light path 205, and an angle α is formed between the LED emission light path 204 and the solution excitation light path 205, where 30° < α < 180°.

[0026] As a preferred implementation, in this embodiment, the angle α between the LED emission light path 204 and the solution excitation light path 205 is 120°.

[0027] In this embodiment, the angle α between the LED emission light path 204 and the solution excitation light path 205 is set to 120°, which can enable the components of the LED emission light path 204 and the components of the solution excitation light path 205 to be closely arranged without occupying too much space.

[0028] Further, in this embodiment, the horizontal height of the opening 206 of the light isolation bottom case 201 is higher than the horizontal height of the components of the LED emission light path 204 and the components of the solution excitation light path 205. Thus, the transmission channels of the LED emission light path 204 and the solution excitation light path 205 can be on one housing, which can exclude the influence of external light on the internal light path.

[0029] Further, in this embodiment, a rabbet 207 is formed by the opening 206 of the light isolation bottom case 201 protruding upward, and a groove corresponding to the rabbet 207 is provided on the light isolation upper cover 202.

[0030] In this embodiment, by providing the rabbet 207 at the opening 206 of the light-shielding bottom case 201 and providing a groove corresponding to the rabbet 207 on the light-shielding top cover 202, light leakage can be prevented, and the accuracy and detection efficiency of fluorescence quantitative detection can be further improved.

[0031] In this embodiment, the components of the LED emission optical path 204 include an LED light source 208, an input filter 209, and an input sensor 210 arranged in sequence from front to back. The solution excitation optical path 205 includes an output filter 211 and an output sensor 212 arranged in sequence from front to back. The test tube placement position 213 is located between the input sensor 210 and the output filter 211.

[0032] The beneficial effect of the fluorescence quantitative detection module with multi-channel light shielding of the present utility model is:

[0033] Through the above technical solution, on the basis of ensuring the accuracy of a single fluorescence quantitative detection channel, the present utility model increases multiple fluorescence quantitative detection channels, which can improve the fluorescence quantitative detection efficiency. It can operate in a single channel or in multiple channels. When operating in multiple channels, the light between the fluorescence quantitative detection channels is shielded by the light-shielding wall to ensure that each channel operates without interference, thereby ensuring the accuracy of the detection results of each fluorescence quantitative detection channel.

[0034] The above are only the preferred embodiments of the present utility model, and do not limit the patent scope of the present utility model accordingly. Any equivalent structural transformation made under the concept of the present utility model by using the content of the specification and drawings of the present utility model, or any direct / indirect application in other related technical fields, is included in the patent protection scope of the present utility model.

Claims

1. A fluorescence quantitative detection module with multi-channel light isolation, characterized in that: include: A light-isolating bottom shell and a light-isolating upper cover arranged on the light-isolating bottom shell, wherein at least two fluorescence quantitative detection channels are arranged on the light-isolating bottom shell, the detection light paths of the at least two fluorescence quantitative detection channels are linearly and closely arranged, and light-isolating walls are arranged between adjacent fluorescence quantitative detection channels.

2. The fluorescence quantitative detection module with multi-channel light isolation according to claim 1, characterized in that: Each of the fluorescence quantitative detection channels is provided with an LED emission light path and a solution excitation light path, a test tube placement position is provided between the LED emission light path and the solution excitation light path, and an angle α is formed between the LED emission light path and the solution excitation light path, wherein 30°<α<180°.

3. The fluorescence quantitative detection module with multi-channel light isolation according to claim 2, characterized in that: The angle α between the LED emission light path and the solution excitation light path is 120°.

4. The fluorescence quantitative detection module with multi-channel light isolation according to claim 3, characterized in that: The horizontal height of the opening of the light-isolating bottom shell is higher than the horizontal heights of the components of the LED emission light path and the components of the solution excitation light path.

5. The fluorescence quantitative detection module with multi-channel light isolation according to claim 4, characterized in that: The opening of the light-isolating bottom shell is upwardly protruded to form a stopper, and the light-isolating upper cover is provided with a groove corresponding to the stopper.

6. The fluorescence quantitative detection module with multi-channel light isolation according to claim 5, characterized in that: The components of the LED emission light path include an LED light source, an input filter and an input sensor arranged in sequence from front to back, and the solution excitation light path includes an output filter and an output sensor arranged in sequence from front to back.

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