Cross contamination prevention ventilation device for nucleic acid extraction

Abstract

The utility model belongs to the technical field of nucleic acid extraction, and particularly relates to a cross contamination prevention ventilation device for nucleic acid extraction. Comprising an extraction box, a fan, an air inlet pipe, a centrifugal fan and an air pipe seat, an air inlet cavity and an exhaust cavity are formed in the extraction box, an extraction pipe is arranged in the air inlet cavity, the fan is communicated with the air inlet cavity through the air inlet pipe, the centrifugal fan is communicated with the exhaust cavity through the air pipe seat, and the air inlet cavity is communicated with the exhaust cavity. Fresh air is exhausted into the air inlet cavity through the fan and the air inlet pipe, so that aerosol containing nucleic acid molecules in the air inlet cavity is blown into the air outlet cavity, and the aerosol containing the nucleic acid molecules in the air outlet cavity is sucked into the air pipe base through the centrifugal fan. By arranging the fan, the air inlet pipe, the centrifugal fan and the air pipe base, aerosol containing nucleic acid molecules in the extraction box can be blown out, cross contamination of samples used for nucleic acid detection in all the extraction pipes in the air inlet cavity is avoided, and therefore the accuracy of nucleic acid detection can be guaranteed.

Description

Technical Field

[0001] The utility model belongs to the technical field of nucleic acid extraction, and particularly relates to an anti-cross-contamination ventilation device for nucleic acid extraction. Background Art

[0002] Nucleic acid detection is an advanced biological detection at the molecular level, which has significant advantages such as higher sensitivity, specificity, and no window period compared with traditional morphological detection, cytological detection, immunological detection, etc.

[0003] However, during the extraction process, the aerosol containing nucleic acid molecules generated in each extraction tube may run into adjacent extraction tubes, which will affect the accuracy of nucleic acid detection. Therefore, it is necessary to design an anti-cross-contamination ventilation device for nucleic acid extraction to solve the above problems. Content of the Utility Model

[0004] In view of the above problems, the utility model provides an anti-cross-contamination ventilation device for nucleic acid extraction to solve the problems raised in the above background art.

[0005] To achieve the above purpose, the utility model provides the following technical solution: An anti-cross-contamination ventilation device for nucleic acid extraction, including an extraction box, a fan, an air inlet pipe, a centrifugal fan, and an air duct seat. An air inlet cavity and an air exhaust cavity are arranged in the extraction box. The air inlet cavity is used for arranging extraction tubes. The fan is used to communicate with the air inlet cavity through the air inlet pipe. The centrifugal fan is used to communicate with the air exhaust cavity through the air duct seat. The air inlet cavity is communicated with the air exhaust cavity.

[0006] Further, the anti-cross-contamination ventilation device for nucleic acid extraction further includes an air inlet partition. The air inlet partition is arranged at the upper end of the extraction box. A plurality of sub-ports are opened on the air inlet partition. There are a plurality of air inlet cavities and a plurality of air exhaust cavities. One end of each of the plurality of sub-ports is communicated with the air inlet pipe, and the other end of each of the plurality of sub-ports is correspondingly communicated with one of the plurality of air inlet cavities. The plurality of air inlet cavities and the plurality of air exhaust cavities are alternately distributed. The air inlet pipe is used to communicate with the upper ends of the plurality of air inlet cavities. The lower ends of the plurality of air inlet cavities are communicated with the lower ends of the plurality of air exhaust cavities. The upper ends of the plurality of air exhaust cavities are communicated with the air duct seat.

[0007] Further, the anti-cross-contamination ventilation device for nucleic acid extraction further includes a sealing sliding sleeve. An inner hole is opened on the air inlet partition. The inner hole is communicated with the air inlet cavity and the sub-port. The sealing sliding sleeve is arranged in the inner hole.

[0008] Further, the anti-cross-contamination ventilation device for nucleic acid extraction further includes an extraction carrier, a sliding sleeve, a push rod, and a spring. The extraction carrier is for the extraction tube, and the extraction carrier is configured to be disposed inside the extraction box. The sliding sleeve is provided with the spring therein. One end of the push rod is connected to the extraction carrier, and the other end of the push rod is slidably connected to the inner wall of the sliding sleeve and abuts against the spring.

[0009] Further, the anti-cross-contamination ventilation device for nucleic acid extraction further includes an air duct cover, a high-efficiency filter cotton, a fan cover, and an air ring. The high-efficiency filter cotton is disposed at the upper end of the air duct seat. The air duct cover covers the air duct seat. The high-efficiency filter cotton is located between the air duct seat and the air duct cover. The centrifugal fan is disposed at the upper end of the air duct cover;

[0010] The fan cover is disposed at the upper end of the air duct cover. The fan cover is used to cover the centrifugal fan. The air ring is disposed at the connection between the upper end of the air duct cover and the fan cover.

[0011] The technical effects and advantages of the present utility model:

[0012] 1. By providing a fan, an air inlet duct, a centrifugal fan, and an air duct seat, the aerosol containing nucleic acid molecules in the extraction box can be blown out, avoiding cross-contamination of the samples for nucleic acid detection in each extraction tube in the air inlet cavity, thereby ensuring the accuracy of nucleic acid detection.

[0013] Other features and advantages of the present utility model will be described in the following description of the specification, and some of them will be obvious from the description of the specification, or will be understood by implementing the present utility model. The objectives and other advantages of the present utility model can be achieved and obtained by the structures pointed out in the specification and the drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the following drawings are some embodiments of the present utility model. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0015] Figure 1 Shows a schematic structural view of the anti-cross-contamination ventilation device for nucleic acid extraction according to an embodiment of the present utility model;

[0016] Figure 2 Shows a schematic structural view of the anti-cross-contamination ventilation device for nucleic acid extraction according to an embodiment of the present utility model from another perspective;

[0017] Figure 3Shows Figure 2 A cross-sectional view in the A-A direction in

[0018] Figure 4 Shows Figure 2 A cross-sectional view in the B-B direction in

[0019] Figure 5 Shows Figure 2 A cross-sectional view in the C-C direction in

[0020] Figure 6 Shows Figure 2 A cross-sectional view in the D-D direction in

[0021] Figure 7 Shows a schematic structural view of another perspective of the anti-cross-contamination ventilation device for nucleic acid extraction according to an embodiment of the present invention;

[0022] Figure 8 Shows Figure 7 A cross-sectional view in the E-E direction in

[0023] Reference numerals: 1, extraction box; 11, intake cavity; 12, exhaust cavity; 21, fan; 22, intake pipe; 23, intake air dividing block; 231, dividing port; 232, inner hole; 24, sealing sliding sleeve; 31, centrifugal fan; 32, air duct seat; 33, air duct cover; 34, high-efficiency filter cotton; 35, fan cover; 36, air ring; 4, extraction tube; 51, extraction carrier; 52, sliding sleeve; 53, push rod; 54, spring; 55, extraction cavity door. Detailed implementation manners

[0024] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0025] As Figures 1 to 8 shown, an anti-cross-contamination ventilation device for nucleic acid extraction according to an embodiment of the present invention includes an extraction box 1, a fan 21, an intake pipe 22, a centrifugal fan 31 and an air duct seat 32. An intake cavity 11 and an exhaust cavity 12 are provided in the extraction box 1. The extraction tube 4 is arranged in the intake cavity 11. The fan 21 is used to communicate with the intake cavity 11 through the intake pipe 22. The centrifugal fan 31 is used to communicate with the exhaust cavity 12 through the air duct seat 32. The intake cavity 11 communicates with the exhaust cavity 12.

[0026] Specifically, a plurality of extraction tubes 4 are arranged in the air inlet cavity 11.

[0027] In this embodiment, by arranging the extraction tube 4 in the air inlet cavity 11, and a sample for nucleic acid detection is arranged in the extraction tube 4. The sample for nucleic acid detection generates an aerosol containing nucleic acid molecules in the air inlet cavity 11, and the aerosol containing nucleic acid molecules may enter other samples for nucleic acid detection, thus affecting the accuracy of nucleic acid detection.

[0028] The fresh air is discharged into the air inlet cavity 11 by using the fan 21 and the air inlet pipe 22, so as to blow the aerosol containing nucleic acid molecules in the air inlet cavity 11 into the exhaust cavity 12, and the aerosol containing nucleic acid molecules in the exhaust cavity 12 is sucked into the air duct base 32 by using the centrifugal fan 31. Thus, by arranging the fan 21, the air inlet pipe 22, the centrifugal fan 31 and the air duct base 32, the aerosol containing nucleic acid molecules in the extraction box 1 can be blown out, avoiding cross-contamination of the samples for nucleic acid detection in each extraction tube 4 in the air inlet cavity, and thus the accuracy of nucleic acid detection can be ensured.

[0029] Optionally, as Figure 1 , Figures 3 to 5 , Figure 7 and Figure 8 shown, the anti-cross-contamination ventilation device for nucleic acid extraction further includes an air inlet partition 23. The air inlet partition 23 is arranged at the upper end of the extraction box 1. A plurality of branch ports 231 are formed on the air inlet partition 23. A plurality of air inlet cavities 11 and a plurality of exhaust cavities 12 are provided. One ends of the plurality of branch ports 231 are communicated with the air inlet pipe 22, and the other ends of the plurality of branch ports 231 are communicated with the plurality of air inlet cavities 11 in one-to-one correspondence. The plurality of air inlet cavities 11 and the plurality of exhaust cavities 12 are alternately distributed. The air inlet pipe 22 is used for communicating with the upper ends of the plurality of air inlet cavities 11. The lower ends of the plurality of air inlet cavities 11 are communicated with the lower ends of the plurality of exhaust cavities 12. The upper ends of the plurality of exhaust cavities 12 are communicated with the air duct base 32.

[0030] Specifically, an extraction cavity is arranged in the extraction box 1. The extraction cavity includes a plurality of air inlet cavities 11 and a plurality of exhaust cavities 12 in the upper space and a communication cavity in the lower space. The plurality of air inlet cavities 11 and the plurality of exhaust cavities 12 are alternately distributed in the left-right direction. The lower end of the air inlet cavity 11 is communicated with the lower end of the exhaust cavity 12 through the communication cavity.

[0031] In this embodiment, by providing a plurality of air inlet chambers 11, an extraction tube 4 can be provided in each air inlet chamber 11. By providing an air inlet partition 23 between the air inlet pipe 22 and the extraction box 1, and opening a plurality of sub-ports 231 on the air inlet partition 23 and correspondingly connecting the plurality of sub-ports 231 to the plurality of air inlet chambers 11, fresh air can be blown into each air inlet chamber 11. Thus, the aerosol containing nucleic acid molecules in the plurality of extraction tubes 4 can be blown out simultaneously.

[0032] Secondly, by connecting the plurality of air inlet chambers 11 and the plurality of air exhaust chambers 12, the aerosol containing nucleic acid molecules in the plurality of air inlet chambers 11 can be blown into the plurality of air exhaust chambers 12 for discharge. Specifically, the aerosol containing nucleic acid molecules can enter the lower end of the adjacent air exhaust chamber 12 through the lower end of the air inlet chamber 11 and be discharged from the upper end of the air exhaust chamber 12.

[0033] Optionally, as Figure 3 shown, the anti-cross contamination ventilation device for nucleic acid extraction further includes a sealing sliding sleeve 24. An inner hole 232 is opened on the air inlet partition 23. The inner hole 232 communicates with the air inlet chamber 11 and the sub-port 231, and the sealing sliding sleeve 24 is arranged in the inner hole 232.

[0034] In this embodiment, by opening an inner hole 232 on the air inlet partition 23 and arranging a sealing sliding sleeve 24 in the inner hole 232, the inner hole 232 is the entrance for the extraction stirring rod sleeve, the stirring rod, and the magnetic rod to enter the extraction chamber, and the sealing sliding sleeve 24 is used for sealing with the extraction stirring rod sleeve.

[0035] Optionally, as Figures 1 to 4 and Figure 7 shown, the anti-cross contamination ventilation device for nucleic acid extraction further includes an extraction carrier 51, a sliding sleeve 52, a push rod 53, and a spring 54. The extraction carrier 51 is for the extraction tube 4, the extraction carrier 51 is arranged inside the extraction box 1, the spring 54 is arranged in the sliding sleeve 52, one end of the push rod 53 is connected to the extraction carrier 51, and the other end of the push rod 53 is slidably connected to the inner wall of the sliding sleeve 52 and abuts against the spring 54.

[0036] Specifically, the extraction carrier 51 is integrally arranged in the communication chamber in the lower space of the extraction chamber, and the extraction tube 4 is correspondingly arranged in the air inlet chamber 11 in the upper space of the extraction chamber. Secondly, an extraction chamber door 55 is provided at the front end of the extraction carrier 51, and the extraction chamber door 55 is hermetically connected to the extraction box 1. In addition, the sliding sleeve 52 is arranged through the base, and a sealing sliding sleeve 24 is arranged between the sliding sleeve 52 and the base.

[0037] In this embodiment, by using the push rod 53 to be slidably connected to the inner wall of the sliding sleeve 52, the extraction carrier 51 can be integrally slidably connected to the extraction box 1, facilitating the setting of the extraction carrier 51 on the inner wall of the extraction box 1, thereby changing the position of the extraction tube 4 and setting it into the air inlet chamber 11. By arranging a spring 54 in the sliding sleeve 52, the extraction carrier 51 can be pushed out by the elastic force of the spring 54, facilitating the removal of the extraction tube 4 from the air inlet chamber 11.

[0038] Optionally, as Figures 1 to 4 and Figure 7 shown, the anti-cross-contamination ventilation device for nucleic acid extraction further includes an air duct cover 33, a high-efficiency filter cotton 34, a fan cover 35, and an air ring 36. The high-efficiency filter cotton 34 is arranged at the upper end of the air duct seat 32. The air duct cover 33 covers the air duct seat 32. The high-efficiency filter cotton 34 is located between the air duct seat 32 and the air duct cover 33. The centrifugal fan 31 is arranged at the upper end of the air duct cover 33;

[0039] The fan cover 35 is arranged at the upper end of the air duct cover 33 for covering the centrifugal fan 31. The air ring 36 is arranged at the connection between the upper end of the air duct cover 33 and the fan cover 35.

[0040] In this embodiment, by arranging the high-efficiency filter cotton 34, the aerosol containing nucleic acid molecules can be filtered by the high-efficiency filter cotton 34, enabling the discharged air to meet the national outdoor emission standards. By arranging the air duct cover 33, the high-efficiency filter cotton 34 can be wrapped and protected. Secondly, by arranging the fan cover 35, the centrifugal fan 31 can be wrapped and protected. Since the air duct cover 33 is large in area, made of sheet metal with a thin sheet metal plate, by arranging the air ring 36, the resonance noise can be effectively reduced.

[0041] Although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements for some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A cross-contamination prevention ventilation device for nucleic acid extraction, characterized in that: The invention comprises an extraction box (1), a fan (21), an air inlet pipe (22), a centrifugal fan (31) and an air duct seat (32); the extraction box (1) is provided with an air inlet chamber (11) and an air exhaust chamber (12); the air inlet chamber (11) is used to be provided with an extraction pipe (4); the fan (21) is used to communicate with the air inlet chamber (11) through the air inlet pipe (22); the centrifugal fan (31) is used to communicate with the air exhaust chamber (12) through the air duct seat (32); and the air inlet chamber (11) is connected with the air exhaust chamber (12).

2. The cross-contamination prevention ventilation device for nucleic acid extraction according to claim 1, characterized in that: The invention also comprises an air inlet block (23), wherein the upper end of the extraction box (1) is provided with the air inlet block (23), and a plurality of branch openings (231) are provided on the air inlet block (23), and the air inlet cavity (11) and the air exhaust cavity (12) are both provided with a plurality of branch openings (231), one end of the plurality of branch openings (231) is connected to the air inlet pipe (22), and the other end of the plurality of branch openings (231) is connected to the plurality of air inlet cavities (11) in a one-to-one correspondence, and the plurality of air inlet cavities (11) and the plurality of exhaust cavities (12) are alternately distributed, and the air inlet pipe (22) is used to be connected to the upper ends of the plurality of air inlet cavities (11), the lower ends of the plurality of air inlet cavities (11) are connected to the lower ends of the plurality of exhaust cavities (12), and the upper ends of the plurality of exhaust cavities (12) are connected to the air duct seat (32).

3. The cross-contamination prevention ventilation device for nucleic acid extraction according to claim 2, characterized in that: It also comprises a sealing sleeve (24); an inner hole (232) is provided on the air inlet block (23); the inner hole (232) is communicated with the air inlet cavity (11) and the branch port (231); and the sealing sleeve (24) is arranged in the inner hole (232).

4. The cross-contamination prevention ventilation device for nucleic acid extraction according to claim 2, characterized in that: It also includes an extraction carrier (51), a sliding sleeve (52), a push rod (53) and a spring (54), wherein the extraction carrier (51) is used for the extraction tube (4), the extraction carrier (51) is used to be arranged inside the extraction box (1), the sliding sleeve (52) is provided with the spring (54), one end of the push rod (53) is connected to the extraction carrier (51), and the other end of the push rod (53) is slidably connected to the inner wall of the sliding sleeve (52) and abuts against the spring (54).

5. The cross-contamination prevention ventilation device for nucleic acid extraction according to claim 1, characterized in that: It also comprises an air duct cover (33), a high-efficiency filter cotton (34), a fan cover (35) and an air ring (36); the high-efficiency filter cotton (34) is arranged at the upper end of the air duct seat (32); the air duct cover (33) is arranged on the air duct seat (32); the high-efficiency filter cotton (34) is located between the air duct seat (32) and the air duct cover (33); and the centrifugal fan (31) is arranged at the upper end of the air duct cover (33); The fan cover (35) is arranged at the upper end of the air duct cover (33), and the fan cover (35) is used to cover the centrifugal fan (31). The air ring (36) is arranged at the connection between the upper end of the air duct cover (33) and the fan cover (35).

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