ATP fluorescence detection device

Abstract

The utility model belongs to the technical field of biological detection, and provides an ATP (Adenosine Triphosphate) fluorescence detection device which comprises a photodiode, a main control module, a signal processing module and a power supply module, the signal processing module comprises an analog-to-digital converter with the resolution not lower than 24 bits and an amplifying circuit; the output end of the photodiode is electrically connected with the input end of the amplification circuit, the output end of the amplification circuit is electrically connected with the input end of the analog-to-digital converter, the output end of the analog-to-digital converter is connected with the main control module, and the main control module and the signal processing module are respectively connected with the power supply module. Aiming at the problem of insufficient accuracy in the prior art, the utility model effectively improves the detection accuracy through a chip with higher resolution.

Description

Technical Field

[0001] The utility model belongs to the technical field of biological detection, and particularly relates to an ATP fluorescence detection device. Background Art

[0002] ATP fluorescence detectors are mainly applied in the fields of food safety and hygiene monitoring, which are crucial for timely and accurately understanding the microbial contamination status in food or the environment. Currently, there are generally two schemes for the detection circuit of ATP fluorescence detectors. The first is to use a photomultiplier tube. The fluorescence generated by exciting adenosine triphosphate can be converted into a relatively strong voltage signal, and then the voltage signal is detected to indirectly obtain the amount of microorganisms and other biological residues in the sample. However, when using a photomultiplier tube, a high-voltage circuit needs to be designed to drive the photomultiplier tube and a heat dissipation system, and its manufacturing cost is high. The second is to use a photodiode. The photodiode works through the photovoltaic effect. The photodiode does not require a complex high-voltage drive and heat dissipation system, but the converted electrical signal is small. The amplification and data processing functions in the detection circuit of the fluorescence detector will directly affect the detection effect and accuracy of ATP.

[0003] The existing technologies mainly focus on realizing signal amplification and processing through traditional analog circuit design, or using low-resolution AD chips for signal processing technology, which to some extent limits the flexibility and accuracy of its detection system. Summary of the Utility Model

[0004] The utility model provides an ATP fluorescence detection device to overcome the defect of poor accuracy in the above-mentioned existing technologies.

[0005] To solve the above technical problems, the technical solution of the utility model is as follows:

[0006] An ATP fluorescence detection device includes: a photodiode, a main control module, a signal processing module, and a power supply module; the signal processing module includes an analog-to-digital converter with a resolution of not less than 24 bits and an amplifier circuit; the output end of the photodiode is electrically connected to the input end of the amplifier circuit, the output end of the amplifier circuit is electrically connected to the input end of the analog-to-digital converter, the output end of the analog-to-digital converter is connected to the main control module, and the main control module and the signal processing module are respectively connected to the power supply module.

[0007] As a preferred solution, the amplifier circuit includes a first operational amplifier circuit and a second operational amplifier circuit. The output end of the photodiode is electrically connected to the input end of the first operational amplifier circuit, the output end of the first operational amplifier circuit is electrically connected to the input end of the analog-to-digital converter, the input end of the second operational amplifier circuit is electrically connected to the output end of the power supply module, and the output end of the second operational amplifier circuit is electrically connected to the input end of the first operational amplifier circuit.

[0008] Preferably, the first operational amplifier circuit includes a first operational amplifier, a first resistor, and a second resistor. The non-inverting input terminal of the first operational amplifier is connected to the output terminal of the photodiode, and the input terminal of the photodiode is connected to the inverting input terminal of the first operational amplifier; the first end of the first resistor is connected to the non-inverting input terminal of the first operational amplifier, the second end of the first resistor is connected to the output terminal of the second operational amplifier circuit, the first end of the second resistor is connected to the inverting input terminal of the first operational amplifier, the second end of the second resistor is connected to the output terminal of the first operational amplifier, and the output terminal of the first operational amplifier is connected to the input terminal of the analog-to-digital converter.

[0009] Preferably, the second operational amplifier circuit includes a second operational amplifier, a third resistor, and a fourth resistor. The first ends of the third resistor and the fourth resistor are respectively connected to the non-inverting input terminal of the second operational amplifier, the second ends of the third resistor and the fourth resistor are respectively connected to the output terminal of the power supply module, and the output terminal of the second operational amplifier is connected to the input terminal of the first operational amplifier circuit.

[0010] Preferably, the power supply module includes: a lithium battery, a boost module, a first buck module, and a second buck module; the input terminal of the boost module is connected to the output terminal of the lithium battery; the input terminal of the first buck module is connected to the output terminal of the boost module, and the output terminal of the first buck module is connected to the power supply terminal of the main control module; the input terminal of the second buck module is connected to the output terminal of the boost module, and the output terminal of the second buck module is connected to the power supply terminal of the signal processing module.

[0011] Preferably, the ATP fluorescence detection device further includes a display module, and the display module includes a touch screen module, a USB interface module, and a conversion module; the conversion module includes a MOS transistor, the gate of the MOS transistor is connected to the power supply module, the drain of the MOS transistor is connected to the touch screen module, and the source of the MOS transistor is connected to the USB interface module.

[0012] Preferably, the analog-to-digital converter includes an AD7190 analog-to-digital converter.

[0013] Preferably, the main control module includes an STM32 single-chip microcomputer.

[0014] Preferably, the ATP fluorescence detection device further includes a temperature detection module, and the temperature detection module is connected to the main control module.

[0015] Preferably, the ATP fluorescence detection device further includes an alarm module, and the alarm module is connected to the main control module.

[0016] Compared with the prior art, the beneficial effects of the technical solution of the utility model are as follows: the utility model first amplifies the electrical signal converted by the photodiode through the signal processing module, and then processes it by an analog-to-digital converter with a resolution of not less than 24 bits. This high-precision analog-to-digital converter can more effectively process weak signals and accurately convert them into digital signals, improving the reliability and accuracy of ATP detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic structural diagram of an ATP fluorescence detection device.

[0018] Figure 2 It is a circuit diagram of a boost module.

[0019] Figure 3 It is a circuit diagram of a first buck module.

[0020] Figure 4 It is a circuit diagram of a second buck module.

[0021] Figure 5 It is a circuit diagram of a charging module.

[0022] Figure 6 It is a circuit diagram of an AD3V module.

[0023] Figure 7 It is a circuit diagram of a USB interface module.

[0024] Figure 8 It is a circuit diagram of a power supply module in a display module.

[0025] Figure 9 It is a circuit diagram of a screen interface circuit.

[0026] Figure 10 It is a circuit diagram of a conversion module.

[0027] Figure 11 It is a circuit diagram of a temperature detection module.

[0028] Figure 12 It is a circuit diagram of an alarm module. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0029] The drawings are only for illustrative purposes and should not be construed as a limitation of this patent;

[0030] To better illustrate this embodiment, some components in the drawings are omitted, enlarged or reduced, and do not represent the dimensions of the actual product;

[0031] For those skilled in the art, it is understandable that some well-known structures and their descriptions in the drawings may be omitted.

[0032] The technical solution of the present utility model will be further described below in conjunction with the accompanying drawings and embodiments.

[0033] Embodiment 1

[0034] This embodiment provides an ATP fluorescence detection device, as Figure 1 shown, which is a schematic structural diagram of the ATP fluorescence detection device.

[0035] The ATP fluorescence detection device proposed in this embodiment includes: a photodiode, a main control module, a signal processing module, and a power supply module; the signal processing module includes an analog-to-digital converter with a resolution of not less than 24 bits and an amplifier circuit; the output end of the photodiode is electrically connected to the input end of the amplifier circuit, the output end of the amplifier circuit is electrically connected to the input end of the analog-to-digital converter, the output end of the analog-to-digital converter is connected to the main control module, and the main control module and the signal processing module are respectively connected to the power supply module.

[0036] In this embodiment, the fluorescence signal is first converted into an electrical signal by the photodiode. After the electrical signal is generated, the amplifier circuit in the signal processing module first amplifies the converted electrical signal. This amplifier circuit can enhance weak electrical signals to ensure the integrity and recognizability of the signal in the subsequent processing; then it is processed by an analog-to-digital converter with a resolution of not less than 24 bits. High-precision conversion is the key to achieving high-sensitivity and high-precision signal processing. Especially when processing weak signals, it can minimize errors and improve the accuracy and repeatability of the signal; subsequently, the signal is sent to the main control module for further processing, and the signal is comprehensively analyzed to obtain accurate detection results.

[0037] In an optional embodiment, the amplifier circuit includes a first operational amplifier circuit and a second operational amplifier circuit. The output end of the photodiode is electrically connected to the input end of the first operational amplifier circuit. The output end of the first operational amplifier circuit is electrically connected to the input end of the analog-to-digital converter. The input end of the second operational amplifier circuit is electrically connected to the output end of the power supply module. The output end of the second operational amplifier circuit is electrically connected to the input end of the first operational amplifier circuit.

[0038] In this embodiment, the electrical signal is amplified by the first operational amplifier circuit, and the second operational amplifier circuit provides a reference voltage for the first operational amplifier circuit. The second operational amplifier circuit can ensure that the first operational amplifier circuit operates at a stable and predetermined voltage level, greatly reducing signal distortion caused by voltage fluctuations or instability of the operational amplifier, thereby optimizing the performance of the entire circuit and improving the accuracy and reliability of signal processing.

[0039] Further optionally, the first operational amplifier circuit includes a first operational amplifier, a first resistor R38, and a second resistor R39. The non-inverting input terminal of the first operational amplifier is connected to the output terminal of the photodiode, and the input terminal of the photodiode is connected to the inverting input terminal of the first operational amplifier; the first end of the first resistor R38 is connected to the non-inverting input terminal of the first operational amplifier, the second end of the first resistor R38 is connected to the output terminal of the second operational amplifier circuit, the first end of the second resistor R39 is connected to the inverting input terminal of the first operational amplifier, the second end of the second resistor R39 is connected to the output terminal of the first operational amplifier, and the output terminal of the first operational amplifier is connected to the input terminal of the analog-to-digital converter.

[0040] In this embodiment, the first operational amplifier circuit amplifies the weak electrical signal received from the photodiode. One end of the first resistor R38 is connected to the non-inverting input terminal of the first operational amplifier, and the other end is connected to the output terminal of the second operational amplifier circuit, thereby introducing a stable reference voltage to the non-inverting terminal, which helps to stabilize the voltage level of the input signal, improve the response speed and stability of the circuit to the signal; the second resistor R39 constitutes a negative feedback loop, which can not only stabilize the output of the amplifier, but also help to suppress possible noise interference, ensuring the accuracy and linearity of the output signal. The output terminal of the first operational amplifier is connected to the input terminal of the analog-to-digital converter, so that the amplified electrical signal is sent to the analog-to-digital converter for further processing.

[0041] Further optionally, the second operational amplifier circuit includes a second operational amplifier, a third resistor R37, and a fourth resistor R41. The first ends of the third resistor R37 and the fourth resistor R41 are respectively connected to the non-inverting input terminal of the second operational amplifier, the second ends of the third resistor R41 and the fourth resistor R41 are respectively connected to the output terminal of the power supply module, and the output terminal of the second operational amplifier is connected to the input terminal of the first operational amplifier circuit.

[0042] In this embodiment, the second operational amplifier circuit is composed of a resistor voltage division network, which can provide a reference voltage for the first operational amplifier circuit, improve the stability of signal processing, and enhance the system's ability to process weak signals.

[0043] Embodiment 2

[0044] This embodiment makes improvements on the basis of the ATP fluorescence detection device proposed in Embodiment 1, such as Figure 2 The circuit diagram of the boost module is shown as Figure 3 The circuit diagram of the first buck module is shown as Figure 4 The circuit diagram of the second buck module is shown as

[0045] The ATP fluorescence detection device proposed in this embodiment includes: a photodiode, a main control module, a signal processing module, and a power supply module; the signal processing module includes an analog-to-digital converter with a resolution of no less than 24 bits and an amplifier circuit; the output end of the photodiode is electrically connected to the input end of the amplifier circuit, the output end of the amplifier circuit is electrically connected to the input end of the analog-to-digital converter, the output end of the analog-to-digital converter is connected to the main control module, and the main control module and the signal processing module are respectively connected to the power supply module.

[0046] In an optional embodiment, the power supply module includes: a lithium battery, a boost module, a first buck module, and a second buck module; the input end of the boost module is connected to the output end of the lithium battery; the input end of the first buck module is connected to the output end of the boost module, and the output end of the first buck module is connected to the power supply end of the main control module; the input end of the second buck module is connected to the output end of the boost module, and the output end of the second buck module is connected to the power supply end of the signal processing module.

[0047] Specifically, the boost module includes a boost chip and an inductor L1. The first end of the inductor L1 is connected to the switch pin of the boost chip, the other end of the inductor L1 is connected to the output end of the lithium battery, and the output end of the lithium battery is connected to the voltage input end of the boost chip; the first buck module includes a low-dropout linear regulator and an inductor L2. The first end of the inductor L2 is connected to the output end of the low-dropout linear regulator, and the second end of the inductor L2 is connected to the voltage input end of the low-dropout linear regulator; the second buck module includes a low-dropout linear regulator, and the output end of the boost module is connected to the voltage input end of the low-dropout linear regulator.

[0048] As an example, the boost chip uses the SX1308 chip, and the low-dropout linear regulator uses the ME6211 chip.

[0049] In this embodiment, the output voltage of the lithium battery is boosted by the boost module, and then different buck modules are used to provide power supplies with different voltages to the main control module and the signal processing module respectively.

[0050] Further optionally, the power supply module further includes a charging module and an AD3V module, as Figure 5 shown is the circuit diagram of the charging module, Figure 6 shown is the circuit diagram of the AD3V module.

[0051] The charging module includes a lithium battery charger for charging the lithium battery; the AD3V module includes a low-dropout voltage regulator for providing a standard power supply of 3V voltage.

[0052] Embodiment 3

[0053] This embodiment makes improvements on the basis of the ATP fluorescence detection device proposed in Embodiment 1 or Embodiment 2. For example, Figure 7 The figure shows the circuit diagram of the USB interface module. Figure 8 The figure shows the circuit diagram of the power supply module in the display module. Figure 9 The figure shows the circuit diagram of the screen interface circuit. Figure 10 It is the circuit diagram of the conversion module.

[0054] The ATP fluorescence detection device proposed in this embodiment includes: a photodiode, a main control module, a signal processing module, and a power supply module; the signal processing module includes an analog-to-digital converter with a resolution of not less than 24 bits and an amplification circuit; the output end of the photodiode is electrically connected to the input end of the amplification circuit, the output end of the amplification circuit is electrically connected to the input end of the analog-to-digital converter, the output end of the analog-to-digital converter is connected to the main control module, and the main control module and the signal processing module are respectively connected to the power supply module.

[0055] In an optional embodiment, the ATP fluorescence detection device further includes a display module, and the display module includes a touch screen module, a USB interface module, and a conversion module; the conversion module includes a MOS transistor, the gate of the MOS transistor is connected to the power supply module, the drain of the MOS transistor is connected to the touch screen module, and the source of the MOS transistor is connected to the USB interface module.

[0056] Specifically, the touch screen module includes a screen interface circuit, and the interface circuit is connected to the touch screen; the USB interface module includes a USB-to-serial port module and a low-dropout linear LDO voltage regulator, and the voltage input end of the low-dropout linear LDO voltage regulator is connected to the output end of the boost module in the power supply module; the voltage output end of the low-dropout linear LDO voltage regulator is connected to the voltage input end of the USB-to-serial port module.

[0057] In this embodiment, the ATP fluorescence detection device is additionally provided with a display module, including a touch screen module, a USB interface module, and a conversion module, and the core of the conversion module is composed of a MOS transistor; through the display module, the human-computer interaction becomes more intuitive and convenient. Among them, when the USB interface is not connected, the touch screen module directly maintains communication with the main control chip, and the user can input control information through the touch screen, and this information is then sent to the main control module. The main control module processes the received control information and sends the corresponding data information back to the touch screen for real-time display; after connecting the USB interface, the system automatically switches through the conversion module to keep the resistive touch screen connected to the USB communication, and at the same time disconnects its communication link with the main control chip, allowing the user to directly access the historical information in the device through the USB interface, improving the convenience and efficiency of data access and meeting the needs of diverse usage scenarios.

[0058] Example 4

[0059] This embodiment makes improvements on the basis of the ATP fluorescence detection device proposed in any one of Embodiments 1 to 3.

[0060] In an alternative embodiment, the analog-to-digital converter includes an AD7190 analog-to-digital converter.

[0061] In this embodiment, the AD7190 is selected as the analog-to-digital converter. The AD7190 is a 24-bit high-precision, low-noise analog-to-digital converter with a programmable data output rate of up to 4.8 kHz, supporting multi-channel differential input and an internal programmable gain amplifier; in addition, the AD7190 analog-to-digital converter also has a synchronous digital filter, which can effectively reduce noise and improve signal quality. By using an analog-to-digital converter with higher resolution, even when there are slight changes in the output voltage of the photodiode, they can be promptly distinguished and displayed.

[0062] Example 5

[0063] This embodiment makes improvements on the basis of the ATP fluorescence detection device proposed in any one of Embodiments 1 to 4.

[0064] In an alternative embodiment, the main control module includes an STM32 single-chip microcomputer.

[0065] In this embodiment, the STM32 single-chip microcomputer used in the main control module has significant technical advantages compared with the old chips used in traditional ATP fluorescence detectors. Compared with the 8-bit or 16-bit microcontrollers used before, it provides a higher processing speed and a larger storage capacity, making the entire detector more efficient when running complex algorithms and improving the detection accuracy; in addition, the advanced communication protocols supported by the STM32 single-chip microcomputer, such as USB, CAN, etc., also provide more flexibility and scalability for the detector.

[0066] Example 6

[0067] This embodiment makes improvements on the basis of the ATP fluorescence detection device proposed in any one of Embodiments 1 to 5, as Figure 11 shown in the circuit diagram of the temperature detection module, and as Figure 12 shown in the circuit diagram of the alarm module.

[0068] In an alternative embodiment, the ATP fluorescence detection device further includes a temperature detection module, and the temperature detection module is connected to the main control module.

[0069] Specifically, the temperature detection module includes a thermistor, the first end of the thermistor is connected to the output end of the first step-down module, and the second end of the thermistor is connected to the main control module.

[0070] In this embodiment, by integrating a temperature detection module into the ATP fluorescence detection device, real-time monitoring of the detection ambient temperature is achieved, thereby significantly improving the accuracy and reliability of the detection; because the sensitivity of ATP detection may vary with the change of ambient temperature. By real-time monitoring and adjusting the detection conditions to adapt to the ambient temperature, false positive or false negative results can be avoided, ensuring the accuracy of the detection data.

[0071] Further optionally, the ATP fluorescence detection device further includes an alarm module, and the alarm module is connected to the main control module.

[0072] Specifically, the alarm module includes a buzzer.

[0073] In this embodiment, by integrating an alarm module into the ATP fluorescence detection device, the safety and response ability of the device when key parameters exceed the preset range can be enhanced. Specifically, when the temperature is too high, this alarm module will give a prompt to help the operator take necessary measures to adjust the ambient temperature, prevent equipment damage or sample degradation caused by abnormal temperature, ensure the effectiveness and reliability of the experimental results, reduce the maintenance cost and the risk of potential equipment failures.

[0074] The same or similar reference numerals correspond to the same or similar components;

[0075] The terms describing the positional relationship in the drawings are only for illustrative purposes and should not be construed as a limitation of this patent;

[0076] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, rather than limitations on the implementation manners of the present invention. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to enumerate all the implementation manners here. Any modifications, equivalent replacements, and improvements made within the spirit and principle of the present invention shall be included in the protection scope of the claims of the present invention.

Claims

1. An ATP fluorescence detection device, characterized in that: include: Photodiode, main control module, signal processing module and power supply module; The signal processing module includes an analog-to-digital converter and an amplifier circuit with a resolution of not less than 24 bits; the output end of the photodiode is electrically connected to the input end of the amplifier circuit, the output end of the amplifier circuit is electrically connected to the input end of the analog-to-digital converter, the output end of the analog-to-digital converter is connected to the main control module, and the main control module and the signal processing module are respectively connected to the power supply module.

2. The ATP fluorescence detection device according to claim 1, characterized in that: The amplifier circuit includes a first operational amplifier circuit and a second operational amplifier circuit, the output end of the photodiode is electrically connected to the input end of the first operational amplifier circuit, the output end of the first operational amplifier circuit is electrically connected to the input end of the analog-to-digital converter, the input end of the second operational amplifier circuit is electrically connected to the output end of the power supply module, and the output end of the second operational amplifier circuit is electrically connected to the input end of the first operational amplifier circuit.

3. The ATP fluorescence detection device according to claim 2, characterized in that: The first operational amplifier circuit includes a first operational amplifier, a first resistor and a second resistor. The non-inverting input terminal of the first operational amplifier is connected to the output terminal of the photodiode, and the input terminal of the photodiode is connected to the inverting input terminal of the first operational amplifier; the first end of the first resistor is connected to the non-inverting input terminal of the first operational amplifier, and the second end of the first resistor is connected to the output terminal of the second operational amplifier circuit; the first end of the second resistor is connected to the inverting input terminal of the first operational amplifier, and the second end of the second resistor is connected to the output terminal of the first operational amplifier; the output terminal of the first operational amplifier is connected to the input terminal of the analog-to-digital converter.

4. The ATP fluorescence detection device according to claim 2, characterized in that: The second operational amplifier circuit includes a second operational amplifier, a third resistor and a fourth resistor, the first ends of the third resistor and the fourth resistor are respectively connected to the in-phase input terminal of the second operational amplifier, the second ends of the third resistor and the fourth resistor are respectively connected to the output terminal of the power supply module, and the output terminal of the second operational amplifier is connected to the input terminal of the first operational amplifier circuit.

5. The ATP fluorescence detection device according to claim 1, characterized in that: The power supply module includes: a lithium battery, a boost module, a first buck module and a second buck module; the input end of the boost module is connected to the output end of the lithium battery; the input end of the first buck module is connected to the output end of the boost module, and the output end of the first buck module is connected to the power supply end of the main control module; the input end of the second buck module is connected to the output end of the boost module, and the output end of the second buck module is connected to the power supply end of the signal processing module.

6. The ATP fluorescence detection device according to claim 1, characterized in that: The ATP fluorescence detection device also includes a display module, which includes a touch screen module, a USB interface module and a conversion module; the conversion module includes a MOS tube, the gate of the MOS tube is connected to the power supply module, the drain of the MOS tube is connected to the touch screen module, and the source of the MOS tube is connected to the USB interface module.

7. The ATP fluorescence detection device according to any one of claims 1 to 6, characterized in that: The analog-to-digital converter includes an AD7190 analog-to-digital converter.

8. The ATP fluorescence detection device according to any one of claims 1 to 6, characterized in that: The main control module includes an STM32 single-chip microcomputer.

9. The ATP fluorescence detection device according to any one of claims 1 to 6, characterized in that: The ATP fluorescence detection device also includes a temperature detection module, and the temperature detection module is connected to the main control module.

10. The ATP fluorescence detection device according to any one of claims 1 to 6, characterized in that: The ATP fluorescence detection device also includes an alarm module, and the alarm module is connected to the main control module.

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