Organ-like microporous hydrogel generation device

Abstract

The utility model discloses an organ-like microporous hydrogel generating device which comprises an outer frame, a plurality of storage chambers are arranged in the outer frame through partition plates, and a first culture dish, a second culture dish and a third culture dish are sequentially placed in the storage chambers. Different numbers of hole grooves are formed in the inner bottom walls of the first culture dish, the second culture dish and the third culture dish, limiting grooves are formed in the outer side of the outer frame and the outer side of the partition plate of the storage chamber, supporting pieces are arranged on the outer sides of the first culture dish, the second culture dish and the third culture dish, and the supporting pieces and the limiting grooves are clamped in a matched mode. According to the utility model, the plurality of storage chambers are arranged in the outer frame through the partition plates, and the first culture dish, the second culture dish and the third culture dish are sequentially clamped in the plurality of storage chambers, so that the plurality of culture dishes can be clamped and positioned, and the plurality of culture dishes can be conveniently subjected to contrast culture observation treatment.

Description

Technical Field

[0001] The utility model relates to the technical field of organoid culture and forming equipment, in particular to an organoid microporous hydrogel generating device. Background Technique

[0002] Organoids are three-dimensional assemblies of cells, containing more than one cell type, and can exhibit some physiological characteristics of the organs to which the cells belong. Organoids are widely used in basic research and disease modeling, greatly improving the experimental level. When conducting comparative analysis, it is often necessary to culture multiple types of cells on the same culture plate simultaneously. The organoid technology has been growing rapidly. In the existing laboratory organoid culture system, well plates or culture dishes are used to culture organoids. We have invented a hydrogel system for forming organoids targeting well plates or dishes.

[0003] In the prior art, there is a culture plate device with the application number CN202122685833.4 for observing living cells in 3D brain organoids. With the above structure, an operator can splice different numbers of culture units according to needs to form a culture plate, thereby effectively controlling the number of culture cavities on the culture plate. And through the pipetting chamber, piston, piston shaft and handle, cell culture medium can be added to several culture cavities on the same culture unit more evenly. However, the positioning stability of multiple hydrogel generating culture dishes is poor, and it is inconvenient to disassemble, assemble and use, and the flexibility of assembling and using multiple culture dishes is poor. Content of the Utility Model

[0004] The purpose of the utility model is to provide an organoid microporous hydrogel generating device to solve the problems in the prior art that the positioning stability of multiple hydrogel generating culture dishes is poor, it is inconvenient to disassemble, assemble and use, and the flexibility of assembling and using multiple culture dishes is poor.

[0005] To achieve the above purpose, the utility model provides the following technical solution: An organoid microporous hydrogel generating device includes an outer frame. Inside the outer frame, multiple storage chambers are arranged through partition plates, and a first culture dish, a second culture dish and a third culture dish are sequentially placed in the multiple storage chambers. Different numbers of hole grooves are provided on the inner bottom walls of the first culture dish, the second culture dish and the third culture dish. Limiting grooves are opened on the outer frame and the outer sides of the partition plates of the storage chamber. Support members are arranged on the outer sides of the first culture dish, the second culture dish and the third culture dish, and the support members are cooperatively clamped with the limiting grooves.

[0006] Further, the outer frame is made of a metal frame, and multiple partition plates are perpendicularly welded to the inner side of the outer frame.

[0007] Further, four support members are equidistantly arranged on the outer side of the culture dish, and a magnetic block is arranged at one end of the support member.

[0008] Further, the magnetic blocks are arranged as rectangular blocks, and the magnetic blocks arranged opposite to each other at 180 degrees have opposite magnetic polarities.

[0009] Further, a plurality of the hole grooves are annularly arranged on the inner bottom walls of the first culture dish, the second culture dish and the third culture dish, and the hole grooves are hexagonal grooves.

[0010] Further, the number of the hole grooves on the inner bottom wall of the second culture dish is twice that of the hole grooves in the first culture dish, and the number of the hole grooves on the inner bottom wall of the third culture dish is twice that of the hole grooves in the second culture dish.

[0011] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0012] 1. Since a plurality of storage chambers are arranged inside the outer frame of the present utility model through partition plates, and the first culture dish, the second culture dish and the third culture dish are sequentially clamped and placed in the plurality of storage chambers, the clamping and positioning of a plurality of culture dishes can be carried out, which is convenient for the comparative culture and observation of a plurality of culture dishes. Moreover, limiting grooves are formed on the outer sides of the outer frame and the partition plates of the storage chamber, and support members are arranged on the outer sides of the first culture dish, the second culture dish and the third culture dish, and the support members are clamped and matched with the limiting grooves, so that the first culture dish, the second culture dish and the third culture dish are highly stable in positioning inside the outer frame and are convenient and quick to disassemble and assemble.

[0013] 2. Different numbers of hole grooves are provided on the inner bottom walls of the first culture dish, the second culture dish and the third culture dish of the present utility model. The number of the hole grooves on the inner bottom wall of the second culture dish is twice that of the hole grooves in the first culture dish, and the number of the hole grooves on the inner bottom wall of the third culture dish is twice that of the hole grooves in the second culture dish. The hole grooves are hexagonal grooves, so that the culture generation with different numbers of hole grooves can be satisfied, and the hydrogel is convenient to form, has a good effect, accelerates the forming quantity of the organoids, and increases the uniformity of the forming quality.

[0014] 3. The outer frame of the present utility model is made of a metal frame, and a plurality of partition plates are perpendicularly welded to the inner side of the outer frame. Four support members are equidistantly arranged on the outer side of the culture dish, and a magnetic block is arranged at one end of the support member. When the support member is clamped in the limiting groove, the magnetic block can adsorb to the outer frame and the partition plates made of metal materials, which is beneficial to improving the stability of the culture dish clamped in the limiting groove through the support member. Moreover, a plurality of culture dishes can be arranged in an attractable and arranged manner with each other through the magnetic blocks, which improves the flexibility of the organoid microporous hydrogel culture generation. Description of the Drawings

[0015] The drawings are used to provide further understanding of the present utility model, and constitute a part of the specification. They are used together with the embodiments of the present utility model to explain the present utility model, and do not constitute a limitation to the present utility model. In the drawings:

[0016] Figure 1It is the top view of the overall structure of the present utility model;

[0017] Figure 2 It is the schematic structural diagram of the culture dish of the present utility model;

[0018] Figure 3 It is the schematic structural diagram of the separate connection of multiple culture dishes of the present utility model.

[0019] In the figure: 1. Outer frame; 2. First culture dish; 3. Second culture dish; 4. Third culture dish; 5. Support member; 6. Magnet block; 7. Hole groove; 8. Storage chamber; 9. Partition board; 10. Limit groove. Specific implementation manner

[0020] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0021] Please refer to Figure 1 , Figure 2 , Figure 3 , in the embodiment of the present utility model, an organoid micro - pore hydrogel generating device includes an outer frame 1. Inside the outer frame 1, a plurality of storage chambers 8 are provided through a partition board 9, and a first culture dish 2, a second culture dish 3, and a third culture dish 4 are sequentially clamped and placed in the plurality of storage chambers 8, so as to be able to perform clamping and positioning processing on the plurality of culture dishes, facilitating the comparative culture and observation processing of the plurality of culture dishes. Different numbers of hole grooves 7 are provided on the inner bottom walls of the first culture dish 2, the second culture dish 3, and the third culture dish 4. The number of hole grooves 7 on the inner bottom wall of the second culture dish 3 is twice the number of hole grooves 7 in the first culture dish 2, and the number of hole grooves 7 on the inner bottom wall of the third culture dish 4 is twice the number of hole grooves 7 in the second culture dish 3, so as to meet the use requirements for the generation of different numbers of hole - groove cultures. Limit grooves 10 are opened on the outer frame 1 and the outer side of the partition board 9 of the storage chamber 8. Support members 5 are provided on the outer sides of the first culture dish 2, the second culture dish 3, and the third culture dish 4, and the support members 5 are clamped and matched with the limit grooves 10, so that the first culture dish 2, the second culture dish 3, and the third culture dish 4 have high stability in positioning within the outer frame 1 and are convenient and fast to disassemble and assemble.

[0022] Preferably, the outer frame 1 is made of a metal frame, and a plurality of partitions 9 are perpendicularly welded to the inner side of the outer frame 1. Four support members 5 are equidistantly arranged outside the culture dish, and a magnetic block 6 is provided at one end of the support member 5. The magnetic block 6 is arranged as a rectangular block, and the magnetic blocks 6 arranged in a 180-degree opposite direction have opposite magnetic properties. When the support member 5 is clamped in the limiting groove 10, the magnetic block can adsorb to the outer frame and partition made of metal material, which is beneficial to improving the stability of the culture dish clamped in the limiting groove 10 through the support member 5. Moreover, multiple culture dishes can be arranged in an attracting manner through the magnetic blocks, improving the flexibility of the generation of organoid microporous hydrogels.

[0023] Preferably, a plurality of hole grooves 7 are annularly arranged on the inner bottom walls of the first culture dish 2, the second culture dish 3, and the third culture dish 4, and the hole grooves 7 are hexagonal grooves, which facilitates the cultivation of organoids through the culture dish.

[0024] The working principle and usage process of the present utility model: Since a plurality of storage chambers 8 are provided inside the outer frame 1 through the partition 9, and the first culture dish 2, the second culture dish 3, and the third culture dish 4 are sequentially clamped and placed in the plurality of storage chambers 8, it is possible to perform clamping and positioning processing on a plurality of culture dishes, facilitating the comparative cultivation and observation processing of a plurality of culture dishes. Moreover, limiting grooves 10 are opened on the outer sides of the outer frame 1 and the partition 9 of the storage chamber 8, and the outer sides of the first culture dish 2, the second culture dish 3, and the third culture dish 4 are clamped with the limiting grooves 10 through the support members 5, so that the first culture dish 2, the second culture dish 3, and the third culture dish 4 have high stability in being positioned inside the outer frame 1, and the disassembly and assembly are convenient and fast.

[0025] Finally, it should be noted that the above are only the preferred embodiments of the present utility model and are not used to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements on some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present utility model shall be included within the protection scope of the present utility model.

Claims

1. An organoid microporous hydrogel production device, comprising an outer frame (1), characterized in that: The outer frame (1) is provided with a plurality of storage chambers (8) via a partition (9), and a first culture dish (2), a second culture dish (3) and a third culture dish (4) are sequentially placed in the plurality of storage chambers (8); the inner bottom walls of the first culture dish (2), the second culture dish (3) and the third culture dish (4) are provided with different numbers of holes (7); the outer sides of the outer frame (1) and the partition (9) of the storage chambers (8) are provided with limiting grooves (10); the outer sides of the first culture dish (2), the second culture dish (3) and the third culture dish (4) are provided with support members (5), and the support members (5) are locked in cooperation with the limiting grooves (10).

2. The organoid microporous hydrogel generating device according to claim 1, characterized in that: The outer frame (1) is a metal frame, and a plurality of partitions (9) are vertically welded to the inner side of the outer frame (1).

3. The organoid microporous hydrogel generation device according to claim 1, characterized in that: Four support members (5) are arranged at equal intervals on the outside of the culture dish, and a magnetic block (6) is arranged at one end of the support member (5).

4. The organoid microporous hydrogel generating device according to claim 3, characterized in that: The magnetic blocks (6) are arranged in rectangular blocks, and the magnetic properties of the magnetic blocks (6) arranged 180 degrees opposite to each other are opposite.

5. The organoid microporous hydrogel generation device according to claim 1, characterized in that: The hole grooves (7) are provided in a plurality of annular shapes on the inner bottom walls of the first culture dish (2), the second culture dish (3) and the third culture dish (4), and the hole grooves (7) are hexagonal grooves.

6. The organoid microporous hydrogel generation device according to claim 5, characterized in that: The number of the holes and grooves (7) on the bottom wall of the second culture dish (3) is twice the number of the holes and grooves (7) on the bottom wall of the first culture dish (2), and the number of the holes and grooves (7) on the bottom wall of the third culture dish (4) is twice the number of the holes and grooves (7) on the bottom wall of the second culture dish (3).

Images