Angle modulation type SPR sensor and SPR detection equipment

Abstract

The angle modulation type SPR sensor comprises a base, the upper end of the base is fixedly connected with the bottom of a transverse moving device, the top of the transverse moving device is fixedly connected with a reaction device, and the top of the reaction device is in sliding contact with the bottom of a prism. The utility model has the beneficial effects that the existing optical film only irradiates at one position in one direction under the irradiation of laser, but the SPR angle switching device can change the position to irradiate the optical film and switch different positions of the optical film, so that the SPR angle switching effect is greatly improved, and the SPR angle switching effect is greatly improved. And the surface of the gold film is not subjected to long-term irradiation heating and oxidation reaction, so that the service life of the light film is further prolonged, and the service life of detection equipment is prolonged.

Description

Technical Field

[0001] The utility model mainly relates to the technical field of copper melting furnaces, and specifically relates to an angle-modulated SPR sensor and an SPR detection device. Background Technique

[0002] The SPR technology, namely the surface plasmon resonance technology, is a powerful label-free method that can be used to detect macromolecular interactions, such as protein-protein interactions, protein-ligand, and drug-receptor interactions. In this technology, an interacting molecule called a "ligand" binds to the chip surface, and another molecule called an "analyte" is delivered to the surface in a continuous flow through a complex microfluidic system. Biomolecules can be attached to the chip surface by means of covalent fixation, high-affinity capture, or hydrophobic adsorption.

[0003] During the detection process of existing SPR detection devices using SPR sensors, a gold film needs to be used. However, the gold film is affected by light sources such as lasers. The gold film on the SPR sensor will be affected by the laser for a long time, and heat and oxidation reactions will occur on the surface of the gold film. The laser energy is concentrated in a small area on the metal surface. Under the irradiation of the laser beam, local melting will occur on the metal surface, making the metal surface uneven, so it will affect the SPR sensor to present errors in the intermolecular reaction. Summary of the Utility Model

[0004] The technical solution of the utility model aims at the technical problem that the existing technical solutions are too single, and provides a solution significantly different from the existing technology. It mainly provides an angle-modulated SPR sensor and an SPR detection device to solve the technical problems proposed in the above background technique.

[0005] The technical solution adopted by the utility model to solve the above technical problems is as follows: an angle-modulated SPR sensor, including a base, the upper end of the base is fixedly connected to the bottom of a lateral moving device, the top of the lateral moving device is fixedly connected to a reaction device, the top of the reaction device is in sliding contact with the bottom of a prism, and both sides of the prism are fixedly connected to a receiving device and an incident light source device respectively.

[0006] Preferably, a groove is opened at the top of the base, the groove is slidably connected to a sliding plate, a first threaded hole is opened on one side of the sliding plate, the first threaded hole is threadedly connected to a first screw rod, one end of the first screw rod is fixedly connected to the output end of a first driving motor, and the first driving motor is fixedly connected to the base.

[0007] Preferably, the lateral movement device includes a second driving motor. One end of the output of the second driving motor is fixedly connected to one end of a second screw rod. The second screw rod is in threaded connection with a screw sleeve. The other end of the second screw rod is fixedly connected to a limiting block. Both ends of the screw sleeve are fixedly connected to one end of a connecting rod. The other end of the connecting rod is fixedly connected to the reaction device.

[0008] Preferably, circular holes are formed at both ends of the screw sleeve. Ball bearings are arranged in the circular holes. The ball bearings are limited by one end of the connecting rod. The ball bearings are in sliding contact with the threads of the second screw rod.

[0009] Preferably, the reaction device includes a reaction block. A through rectangular groove is formed in the reaction block. One end of the rectangular groove communicates with a guiding port. The other end of the rectangular groove communicates with one end of a first drainage pipe. The other end of the first drainage pipe is fixedly connected to the connecting rod. A square opening is formed at the top of the reaction block. The square opening communicates with the rectangular groove. The inner wall of the square opening is fixedly connected to the outer ring of the gold film.

[0010] Preferably, a circular hole is formed on one side of the first drainage pipe. The circular hole communicates with a second drainage pipe.

[0011] Preferably, the SPR detection device includes the above-mentioned angle modulation type SPR sensor.

[0012] Compared with the prior art, the beneficial effects of the present utility model are as follows: The driving motor drives the first screw rod to rotate. The first screw rod causes the sliding plate to slide along the groove, thereby causing the reaction device on the lateral movement device to move. As a result, the incident light source device, the prism, and the receiving device receive the reaction conditions at different positions of the gold film in the reaction device. Thus, the laser beam no longer only irradiates one place on the gold film, but continuously switches positions, thereby prolonging the service life of the gold film.

[0013] The second driving motor drives the second screw rod to rotate, thereby driving the screw sleeve, the connecting rod, and the reaction device. As a result, the incident light source device, the prism, and the receiving device receive the reaction conditions at different positions of the gold film in the reaction device, thereby further and more comprehensively changing the irradiation position of the gold film.

[0014] Hereinafter, the present utility model will be explained and described in detail in combination with the accompanying drawings and specific embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 is a three-dimensional schematic diagram of the present utility model;

[0016] Figure 2 is a structural schematic diagram of the lateral movement device in the present utility model;

[0017] Figure 3 is a schematic cross-sectional structure diagram of the screw sleeve in the present utility model.

[0018] Markings in the figure: 1 - base, 11 - first drive motor, 12 - sliding plate, 13 - first screw, 2 - lateral movement device, 21 - second drive motor, 22 - second screw, 23 - screw sleeve, 231 - ball, 24 - limit block, 25 - connecting rod, 3 - reaction device, 31 - reaction block, 32 - guiding port, 33 - first drainage tube, 34 - second drainage tube, 35 - gold film, 4 - receiving device, 5 - incident light source device, 6 - prism. Specific embodiments

[0019] To facilitate the understanding of the present utility model, the present utility model will be described more comprehensively below with reference to the relevant drawings. Several embodiments of the present utility model are given in the drawings. However, the present utility model can be implemented in different forms and is not limited to the embodiments described in the text. On the contrary, these embodiments are provided to make the disclosure of the present utility model more thorough and comprehensive.

[0020] It should be noted that when an element is referred to as "fixedly provided on" another element, it can be directly on the other element or there can be an intermediate element. When an element is considered to be "connected" to another element, it can be directly connected to the other element or there may be an intermediate element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are only for the purpose of illustration.

[0021] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the technical field to which the present utility model belongs. The terms used in the specification of the present utility model herein are only for the purpose of describing specific embodiments and are not intended to limit the present utility model. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0022] Please refer to Figure 1 , an angle modulation type SPR sensor and an SPR detection device, comprising a rectangular base 1. The upper end of the base 1 is fixedly connected to the bottom of the lateral movement device 2. The top of the lateral movement device 2 is fixedly connected to the reaction device 3. The top of the reaction device 3 is in sliding contact with the bottom of the prism 6. The two sides of the prism 6 are respectively fixedly connected to the receiving device 4 and the incident light source device 5.

[0023] Please refer to Figure 2, a groove is formed at the top of the base 1. The groove is slidably connected to the sliding plate 12. A first threaded hole is formed on one side of the sliding plate 12. The first threaded hole is threadedly connected to the first screw rod 13. One end of the first screw rod 13 is fixedly connected to the output end of the first driving motor 11. The first driving motor 11 is fixedly connected to the base 1. By driving the first screw rod 13 to rotate through the driving motor 11, the first screw rod 13 causes the sliding plate 12 to slide along the groove, thereby causing the reaction device 3 on the lateral movement device to move, thereby causing the incident light source device 5, the prism 6, and the receiving device 4 to receive the reaction conditions at different positions of the gold film 35 in the reaction device 3, thereby causing the laser beam to no longer irradiate only one place of the gold film 35, thereby continuously switching positions, and thereby extending the service life of the gold film 35.

[0024] The lateral movement device 2 includes a second driving motor 21. The output end of the second driving motor 21 is fixedly connected to one end of the second screw rod 22. The second screw rod 22 is threadedly connected to the screw sleeve 23. The other end of the second screw rod 22 is fixedly connected to the limiting block 24. Both ends of the screw sleeve 23 are fixedly connected to one end of the connecting rod 25. The other end of the connecting rod 25 is fixedly connected to the reaction device 3. By driving the second screw rod 22 to rotate through the second driving motor 21, the screw sleeve 23, the connecting rod 25, and the reaction device 3 are driven, thereby causing the incident light source device 5, the prism 6, and the receiving device 4 to receive the reaction conditions at different positions of the gold film 35 in the reaction device 3, and thereby further changing the irradiation position of the gold film 35 more comprehensively. The reaction device 3 includes a reaction block 31. A through rectangular groove is formed in the reaction block 31. One end of the rectangular groove communicates with the guiding port 32. The other end of the rectangular groove communicates with one end of the first drainage pipe 33. The other end of the first drainage pipe 33 is fixedly connected to the connecting rod 25. A square opening is formed at the top of the reaction block. The square opening communicates with the rectangular groove. The inner wall of the square opening is fixedly connected to the outer ring of the gold film 35. Molecules enter through the guiding port 32, and then the molecules enter the reaction block 31 and contact the molecules at the lower end of the gold film 35, and finally are led out from the first drainage pipe 33. A round hole is formed on one side of the first drainage pipe 33. The round hole communicates with the second drainage pipe 34. Through the second drainage pipe 34, the molecules can be led out of the base 1, thereby affecting the normal operation of the devices on the base 1.

[0025] Please refer to Figure 3 , round holes are formed at both ends of the screw sleeve 23. Ball bearings 231 are arranged in the round holes. The ball bearings 231 are limited by one end of the connecting rod 25. The ball bearings 231 are in threaded sliding contact with the thread on the outer circle of the second screw rod 22. By means of the ball bearings 231, the wear of the thread on the outer circle of the second screw rod 22 can be slowed down, thereby extending the service life of the screw rod 22.

[0026] The present invention also provides an SPR detection device, including the above-mentioned angle modulation type SPR sensor.

[0027] The above has given an exemplary description of the present utility model in conjunction with the accompanying drawings. Obviously, the specific implementation of the present utility model is not limited by the above-mentioned manner. As long as such non-substantial improvements are made by adopting the method concept and technical solution of the present utility model, or the concept and technical solution of the present utility model are directly applied to other occasions without improvement, they are all within the protection scope of the present utility model.

Claims

1. An angle-modulated SPR sensor, characterized in that: The invention comprises a base (1), the upper end of the base (1) is connected and fixed to the bottom of a transverse moving device (2), the top of the transverse moving device (2) is connected and fixed to a reaction device (3), the top of the reaction device (3) is in sliding contact with the bottom of a prism (36) (6), and the two sides of the prism (36) (6) are respectively connected and fixed to a receiving device (4) and an incident light source device (5).

2. An angle-modulated SPR sensor according to claim 1, characterized in that: A groove is provided on the top of the base (1), and the groove is slidably connected to the sliding plate (12). A first threaded hole is provided on one side of the sliding plate (12), and the first threaded hole is threadedly connected to the first screw rod (13). One end of the first screw rod (13) is connected and fixed to the output end of the first drive motor (11), and the first drive motor (11) is connected and fixed to the base (1).

3. The angle-modulated SPR sensor according to claim 1, characterized in that: The lateral moving device (2) comprises a second driving motor (21), the output end of the second driving motor (21) is connected and fixed to one end of a second screw rod (22), the second screw rod (22) is threadedly connected to a screw sleeve (23), the other end of the second screw rod (22) is connected and fixed to a limit block (24), both ends of the screw sleeve (23) are connected and fixed to one end of a connecting rod (25), and the other end of the connecting rod (25) is connected and fixed to a reaction device (3).

4. The angle-modulated SPR sensor according to claim 3, characterized in that: Circular holes are formed at both ends of the screw sleeve (23), and balls (231) are arranged in the circular holes. The balls (231) are limited by one end of the connecting rod (25), and the balls (231) are in sliding contact with the threads of the second screw rod (22).

5. The angle-modulated SPR sensor according to claim 3, characterized in that: The reaction device (3) comprises a reaction block (31). The reaction block (31) is provided with a rectangular groove extending therethrough. One end of the rectangular groove is connected to a guide port (32). The other end of the rectangular groove is connected to one end of a first drainage tube (33). The other end of the first drainage tube (33) is connected and fixed to a connecting rod (25). A square opening is provided on the top of the reaction block (31). The square opening is connected to the rectangular groove. The inner wall of the square opening is connected and fixed to the outer ring of the gold film (35).

6. The angle-modulated SPR sensor according to claim 5, characterized in that: A circular hole is provided on one side of the first drainage tube (33), and the circular hole is communicated with the second drainage tube (34).

7. An SPR detection device, characterized in that: The SPR detection device comprises the angle-modulated SPR sensor according to any one of claims 1 to 6.

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