Culture equipment

Abstract

The utility model discloses culture equipment which comprises an adsorption device and a culture area used for containing a culture medium, the adsorption device is provided with a containing space used for containing an adsorption material, and the culture area is used for storing a to-be-cultured sample adsorbed by the adsorption material for a preset time. A to-be-cultured sample flows into an adsorption material in the adsorption device, antibiotics in the to-be-cultured sample are adsorbed through the adsorption material, the to-be-cultured sample subjected to adsorption flows into a culture medium in the culture area to be cultured, and in the whole adsorption process, the adsorption material in the adsorption device is separated from the culture medium in the culture area; the non-specific adsorption of a culture substance in the culture medium by an adsorption material is avoided, the culture performance of the culture medium is ensured, the influence of the whole adsorption treatment process on a culture detection result is reduced, and the detection rate of microbiological detection is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of medical devices, in particular to a culture device. Background Art

[0002] Sepsis has become a major health care problem due to its high incidence and high fatality rate in hospitals. A major cause of sepsis is bloodstream infection (BSI). BSI is most commonly diagnosed by blood culture. In hospital clinical and medical research trials, automated blood culture is a microbiological examination method used to detect the presence of bacteria in blood samples. It plays a very important role in quickly detecting whether there is bacterial growth in the blood of patients with sepsis and bacteremia, which seriously endanger the lives of patients clinically, so as to make a clear diagnosis. Therefore, blood culture is one of the most important and common bacterial test items.

[0003] Existing automated blood culture systems can detect the presence of microorganisms in blood within 1 - 48 hours and rule out the presence of microorganisms within 5 days. Usually, blood culture requires additives to ensure as fast and accurate a diagnosis of bloodstream infection as possible. For example, the patient's blood already contains antibiotics at the time of sampling. The presence of antibiotics will further increase the time required to detect microorganisms and even reduce the microorganism detection rate. In addition, an additional 12 - 48 hours is required to confirm the microorganisms by subculturing positive blood cultures and performing identification and antimicrobial susceptibility tests. These results may be too late to change the treatment process, leading to the death of the patient. For other samples such as cerebrospinal fluid, pleural effusion, etc., currently, they are also routinely cultured and enriched by a culture system before subsequent detection.

[0004] Conventional sterility testing methods in the industrial field are used to confirm that sterile products such as sterile pharmaceutical products and sterile medical devices do not contain contaminating microorganisms. Specifically, sterility testing is used to determine whether each batch of sterile products produced during the production process is suitable for release. Therefore, sterility testing is a basic and necessary quality control step before sterile products leave the factory and an essential link to ensure the safety of sterile products. Such industrial products as sterile food and beverages, advanced therapeutic drugs, traditional pharmaceuticals, and biological products will introduce bacteriostatic components such as preservatives or antimicrobial drug residues during the production process. At this time, culturing and testing the sterility of samples will affect the detection rate of contaminants.

[0005] Theoretically, various adsorption resins or activated carbons can be used to adsorb antibiotics, preservatives, or drug residues in samples, thereby shortening the time required to detect the presence of microorganisms and improving the detection rate of microorganism detection. The adsorption resin or activated carbon and the microorganism growth medium are generally mixed in a sealed container. However, through experimental verification, in some cases, adding the adsorption resin or activated carbon will instead cause a decline in culture performance and a decline in the microorganism detection rate.

[0006] Therefore, how to ensure the culture performance of the culture medium and improve the microorganism detection rate is a technical problem that those skilled in the art need to solve currently. Summary of the Invention

[0007] In view of this, the purpose of the present utility model is to provide a culture device to ensure the culture performance of the culture medium and improve the microorganism detection rate.

[0008] To achieve the above purpose, the present utility model provides the following technical solutions:

[0009] A culture device includes an adsorption device and a culture area for holding a culture medium. The adsorption device has a receiving space for placing an adsorption material, and the culture area is used to store a sample to be cultured after being adsorbed by the adsorption material for a preset time.

[0010] Optionally, in the above culture device, a connection channel is provided between the adsorption device and the culture area.

[0011] Optionally, in the above culture device, the adsorption device is provided with an inlet and an outlet both communicating with the receiving space. A sample receiving area for holding a sample to be cultured is communicatively arranged between the inlet and the receiving space. The sample to be cultured is configured to sequentially pass through the sample receiving area, the receiving space, and the outlet from the inlet; and / or,

[0012] There is a pressure difference between the adsorption device and the culture area, and the pressure in the receiving space of the adsorption device is greater than the pressure in the culture area to increase the transfer speed of the sample to be cultured between the adsorption device and the culture area.

[0013] Optionally, in the above culture device, the pressure difference is not greater than 1 Mpa.

[0014] Optionally, in the above culture device, the adsorption device is further provided with a sealing cover for adjusting the covering area of the inlet to control the outflow speed of the sample to be cultured from the outlet; and / or,

[0015] The adsorption device is further provided with a diversion tube communicatively connected to the outlet, so that the sample to be cultured flowing out from the outlet flows into the culture area through the diversion tube.

[0016] Optionally, in the above culture device, the culture area is arranged in the cavity of a culture bottle, and there is a clearance between the adsorption material and the culture area.

[0017] Optionally, in the above-mentioned culture device, the culture flask is provided with a bottle mouth extension part. The bottle mouth of the culture flask communicates with the culture area through the bottle mouth extension part. The adsorption device is provided with an installation opening, so that the bottle mouth extension part extends into the inner cavity of the adsorption device through the installation opening, and the edge of the installation opening is clamped on the outer wall of the bottle mouth extension part. The adsorption material is located between the inflow port and the bottle mouth of the culture flask.

[0018] Optionally, in the above-mentioned culture device, one end of the adsorption device where the outflow port is arranged extends into the inner cavity of the culture flask, faces the culture area, and has the avoidance gap with the culture area. One end of the adsorption device where the inflow port is arranged extends out of the inner cavity of the culture flask; and / or,

[0019] The adsorption device and the culture flask are of an integral structure.

[0020] Optionally, in the above-mentioned culture device, a blocking layer is arranged in the inner cavity of the culture flask. The blocking layer is connected to the inner wall of the culture flask as the adsorption device, and divides the culture flask into two upper and lower chambers. The chamber located below serves as the culture area, and the chamber located above forms the accommodation space. The blocking layer is provided with a plurality of through holes, and the through holes communicate the culture area and the accommodation space.

[0021] Optionally, in the above-mentioned culture device, a partition layer is arranged in the inner cavity of the culture flask. The partition layer divides the inner cavity of the culture flask into a first chamber and a second chamber. The first chamber contains the adsorption material to serve as the adsorption device, and the first chamber corresponds to the bottle mouth of the culture flask. The second chamber avoids the bottle mouth of the culture flask and is used to contain the culture medium to serve as the culture area;

[0022] The first end of the partition layer is connected to the bottom of the culture flask. The second end of the partition layer extends towards the bottle mouth of the culture flask, and a communication port is arranged between the second end of the partition layer and the inner wall of the culture flask. The first chamber and the second chamber are connected through the communication port.

[0023] Optionally, in the above-mentioned culture device, the adsorption device and the culture area are arranged in different regions within the same container. The adsorption device includes an isolation layer that jointly encloses the accommodation space with the inner wall of the container. The isolation layer is provided with an avoidance structure for the sample to be cultured to pass through, and the avoidance structure separates the adsorption material and the culture area;

[0024] The container is rotatable. When the container rotates to the first state, the accommodation space is disposed above the culture area. When the container rotates to the second state, the accommodation space is disposed below the culture area.

[0025] When the culture device provided by the present utility model is in use, first, a sample to be cultured is poured into the adsorption device. The sample to be cultured flows into the adsorption material in the adsorption device, and the antibiotic in the sample to be cultured is adsorbed by the adsorption material. The sample to be cultured after adsorption flows into the culture medium in the culture area for culturing. During the entire adsorption process, the adsorption material in the adsorption device is separated from the culture medium in the culture area. Thus, while completing the adsorption treatment of the antibiotic in the sample to be cultured, non-specific adsorption of the culture substances in the culture medium by the adsorption material is avoided, the culture performance of the culture medium is ensured, the influence of the entire adsorption treatment process on the culture detection result is reduced, and the detection rate of microorganism detection is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present utility model. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0027] Figure 1 It is a schematic structural diagram of the culture device disclosed in the first embodiment of the present utility model;

[0028] Figure 2 It is a schematic structural diagram of the culture device disclosed in the second embodiment of the present utility model;

[0029] Figure 3 It is a schematic structural diagram of the culture device disclosed in the third embodiment of the present utility model;

[0030] Figure 4 It is a schematic structural diagram of the culture device disclosed in the fourth embodiment of the present utility model;

[0031] Figure 5 It is a schematic structural diagram of the culture device disclosed in the fifth embodiment of the present utility model;

[0032] Figure 6 It is a schematic structural diagram of the culture device disclosed in the sixth embodiment of the present utility model;

[0033] Figure 7 It is a schematic structural diagram of the culture device disclosed in the seventh embodiment of the present utility model Figure 1 ;

[0034] Figure 8Structural schematic of the culture device disclosed in the seventh embodiment of the present utility model Figure 2 。

[0035] Among them, 100 is a culture bottle, 110 is a bottle mouth extension part, 120 is a barrier layer, 130 is a partition layer, 140 is a first chamber, 150 is a second chamber, and 160 is an isolation layer;

[0036] 200 is an adsorption device, and 210 is a diversion tube;

[0037] 300 is an adsorption material;

[0038] 400 is a sealing cap. Detailed implementation manners

[0039] The core of the present utility model lies in disclosing a culture device to ensure the culture performance of the culture medium and improve the microorganism detection rate.

[0040] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the protection scope of the present utility model.

[0041] The inventors of the present application found that adsorption resins or activated carbon have the characteristics of non-specific adsorption. Therefore, in addition to being able to adsorb antibiotics, preservatives or drug residues in the sample to be cultured, adsorption resins or activated carbon will also non-specifically adsorb nutrients in the microbial growth medium and the sample to be cultured, including some basic nutrients, amino acids and growth factors, etc. It is precisely due to the adsorption of such substances by adsorption resins or activated carbon that the culture performance of the culture medium is affected, resulting in a decline in culture performance and a decline in the microorganism detection rate. Based on this, the inventors of the present application disclose the following culture methods and culture devices.

[0042] The culture method disclosed in the embodiment of the present utility model adsorbs antibiotics in the blood by adding the adsorption material 300, and specifically includes the following steps:

[0043] Step a: Make the adsorption material 300 contact with the sample to be cultured, and adsorb and cultivate the sample to be cultured by the adsorption material 300 for a preset time.

[0044] Combine Figures 1 - 6, specifically, first, perform an adsorption treatment on the sample to be cultured. Place the sample to be cultured containing antibiotics, preservatives, or drug residues into the adsorption device 200 in the culture equipment, and let the adsorption material 300 in the adsorption device 200 adsorb the antibiotics, preservatives, or drug residues in the sample to be cultured according to the preset adsorption time. The adsorption material 300 and the culture medium do not contact each other before the adsorption culture, which can avoid the loss of the culture substances in the culture medium caused by the contact between the adsorption material 300 and the culture medium and long-term storage, and ensure the culture effect. The preset time can be adjusted according to actual needs.

[0045] Step b: Add a culture medium to the sample to be cultured for sample culture.

[0046] Combine Figures 1 - 5 , after being adsorbed and cultured by the adsorption material 300, the sample to be cultured can flow into the culture medium solution in the culture flask 100 to realize the mixing of the sample to be cultured and the culture medium. At the same time, the separation of the adsorption material 300 and the culture medium during the sample culture process can also be realized. Or, the adsorption material 300 and the sample to be cultured can be directly mixed with the culture medium and sample cultivation can be carried out.

[0047] Compared with the prior art, the culture method disclosed by the present utility model realizes the isolation of the culture medium and the adsorption material 300 during the adsorption culture process by mixing the sample to be cultured with the adsorption material 300 for a preset time and then mixing it with the culture medium for sample culture, avoiding excessive non-specific adsorption of the culture substances in the sample to be cultured by the adsorption material 300 during the adsorption culture process, ensuring the culture performance of the culture medium, enabling the culture medium to provide sufficient nutrition for the sample to be cultured, thereby shortening the time required to detect the presence of microorganisms and improving the detection rate of microorganism detection.

[0048] In addition, the culture method disclosed in the embodiment of the present utility model further includes step c.

[0049] Step c is specifically to separate the sample to be cultured from the adsorption material 300 after being cultured for a preset time to avoid non-specific adsorption of the culture substances in the culture medium by the adsorption material 300 during the sample culture process and ensure that the culture medium can provide sufficient nutrition for the sample to be cultured.

[0050] Specifically, after the adsorption culture of the sample to be cultured reaches the preset time, the sample to be cultured is separated from the adsorption material 300. The separated sample to be cultured is mixed with the culture medium and then placed in an incubator for constant-temperature culture at a preset temperature. After the culture is completed, it is placed in a detection device for detection, and the results of the detection items and the positive alarm time are recorded, and finally the detection is completed.

[0051] Further, before step a, there is also step d.

[0052] Step d: Sterilize the adsorption material 300, the culture medium, and the sample to be cultured.

[0053] Specifically, before placing the sample to be cultured into the culture device, aseptic treatment should be carried out first, and the adsorption material 300 and the culture medium should be sterilized. In a specific embodiment, aseptic treatment can be carried out by means such as high-pressure steam sterilization, cobalt-60 irradiation, electron beam sterilization, ethylene oxide sterilization, etc., to reduce the probability of the sample to be cultured being contaminated by external equipment, thereby improving the accuracy of the test results.

[0054] The above sample to be cultured can specifically be a blood sample, cerebrospinal fluid sample, ascites sample, or other biological samples, as well as industrial samples such as aseptic food and beverages, advanced therapeutic drugs, traditional pharmaceuticals, and biological products. The adsorption material 300 can specifically be an adsorption resin, activated carbon, or other similar substances, as well as their compositions, etc., which will not be listed one by one herein.

[0055] In some embodiments, the preset time for adsorption and cultivation is at least not less than 5 minutes to ensure the adsorption effect on substances such as antibiotics, preservatives, or drug residues in the sample to be cultured. The particle size of the adsorption material of the adsorption material 300 is 1 mesh - 1000 meshes, and the height range of the adsorption layer is 1 cm - 10 cm to ensure the adsorption capacity of the adsorption material 300. Of course, it can be understood that the size of the aperture provided in the adsorption device 200 for the outflow of the sample to be cultured needs to be smaller than the diameter of the particles of the adsorption material 300 to prevent the adsorption material 300 from falling into the culture medium in the culture bottle 100 from the adsorption device 200.

[0056] As Figure 1 shown, the culture device disclosed in the embodiment of the present invention is used for culturing by the above-mentioned culture method, and includes an adsorption device 200 and a culture area for containing the culture medium. The adsorption device 200 has a receiving space for placing the adsorption material 300, and the culture area is used for storing the sample to be cultured after being adsorbed by the adsorption material for a preset time.

[0057] In the specific culture process, the sample to be cultured (or the mixed liquid of the sample to be cultured and the culture medium) enters the receiving space of the adsorption device 200 and comes into direct contact with the adsorption material 300 to be adsorbed by the adsorption material 300 to remove antibiotics. After being adsorbed for a preset time, the sample to be cultured can flow into the culture area (the culture medium can be pre-installed in the culture area) and continue to be cultured by the culture medium. Since the sample to be cultured is cultured by the above-mentioned culture method, it also has the above-mentioned beneficial effects, which will not be elaborated here.

[0058] In order to facilitate the flow of the sample to be cultured from the receiving space to the culture area, a connection channel is provided between the adsorption device 200 and the culture area.

[0059] In one embodiment, the adsorption device 200 is provided with an inlet and an outlet that are both in communication with the accommodation space. A sample accommodation area for holding the sample to be cultured is arranged between the inlet and the adsorption material 300 located in the adsorption device 200 (i.e., between the inlet and the accommodation space). The sample accommodation area can buffer a certain amount of the sample to be cultured, ensuring a continuous supply of the sample to be cultured at the adsorption material 300 in the accommodation space.

[0060] Preferably, a pressure difference is set between the adsorption device 200 and the culture area to facilitate the flow of the sample to be cultured from the adsorption device 200 into the culture area.

[0061] The above-mentioned culture area can be specifically arranged in the cavity of the culture bottle 100, and a clearance is left between the adsorption material 300 and the culture area to avoid interference caused by the contact between the adsorption material 300 and the culture medium in the culture area.

[0062] Specifically, in combination with Figure 1 , the entire inner cavity of the culture bottle 100 serves as the above-mentioned culture area, the culture medium is placed in the culture bottle 100, and the adsorption material 300 for adsorbing the sample to be cultured is placed in the adsorption device 200. And a spacing gap is left between the adsorption material 300 in the adsorption device 200 and the inlet of the adsorption device 200 to form the above-mentioned sample accommodation area, ensuring that the sample to be cultured poured into the adsorption device 200 can be temporarily stored. The sample to be cultured can slowly penetrate into the adsorption material 300, and the adsorption material 300 can preferably adsorb the antibiotics in the sample to be cultured. The adsorbed sample to be cultured can then flow out through the outlet of the adsorption device 200 and into the culture bottle 100, and be mixed into the culture medium in the culture area.

[0063] During the entire adsorption process, since a clearance is provided between the adsorption material 300 in the adsorption device 200 and the culture medium in the culture bottle 100, the separation of the adsorption material 300 from the culture medium is achieved, avoiding the non-specific adsorption of the culture substances in the culture medium by the adsorption material 300, ensuring the culture performance of the culture medium, enabling the culture medium to provide sufficient nutrition for the sample to be cultured, thereby shortening the time required to detect the presence of microorganisms and improving the detection rate of microorganism detection. In addition, after the adsorption material 300 in the adsorption device 200 adsorbs and processes the sample to be cultured, the adsorbed sample to be cultured can directly flow into the culture bottle 100 from the outlet of the adsorption device 200, without the need to separately process the sample to be cultured and the culture medium through multiple containers and then complete the mixing of the filtered sample to be cultured and the culture medium after multiple transfer steps, thereby further reducing the probability of contaminating the sample to be cultured during the multi-step transfer process.

[0064] In a specific embodiment, in combination with Figure 6, the adsorption device 200 is further provided with a sealing cover 400 and / or a diversion tube 210. The sealing cover 400 is used to seal the inlet of the adsorption device 200. By controlling the ratio of the sealing area of the inlet by the sealing cover 400, the flow rate of the sample to be cultured flowing out from the outlet can be controlled. The larger the sealing area, the slower the flow rate of the sample to be cultured flowing out from the outlet. Subsequently, the adsorption time of the adsorption material 300 in the adsorption device 200 for the sample to be cultured can be controlled, so that the adsorption material 300 can achieve a better adsorption effect on the sample to be cultured. At the same time, in order to enable the adsorbed sample to be cultured to flow into the culture bottle 100 better, the culture device disclosed in this embodiment is further provided with a diversion tube 210. One end of the diversion tube 210 is connected to the outlet of the adsorption device 200, and the other end extends into the culture bottle 100. The sample to be cultured flowing out from the outlet of the adsorption device 200 flows into the culture bottle 100 through the diversion tube 210, improving the smoothness and uniformity of the flow of the sample to be cultured. In addition, a switching valve can be provided on the diversion tube 210 to cut off or conduct the diversion tube 210 through the switching valve, so as to control whether the sample to be cultured continues to flow into the culture bottle 100 according to actual needs.

[0065] As Figure 2 shown, in one embodiment, the culture bottle 100 is provided with a bottle mouth extension 110. The bottle mouth of the culture bottle 100 is opened at the end of the bottle mouth extension 110, so that the bottle mouth of the culture bottle 100 is connected to the culture area in the inner cavity of the culture bottle 100 through the bottle mouth extension 110. The adsorption device 200 is provided with an installation opening, so that the bottle mouth extension 110 extends into the inner cavity of the adsorption device 200 through the installation opening, and the edge of the installation opening is clamped on the outer wall of the bottle mouth extension 110 to realize the mutual connection between the culture bottle 100 and the adsorption device 200. At this time, the adsorption material 300 is located between the inlet of the adsorption device 200 and the bottle mouth of the culture bottle 100, so that the adsorbed sample to be cultured can directly flow into the culture bottle 100. Through the above scheme of connecting the culture bottle 100 and the adsorption device 200 to each other, during the adsorption process, the adsorption device 200 can be directly placed on the culture bottle 100, which not only avoids the need to equip a dedicated support structure to support and fix the adsorption device 200, reducing the complexity of the overall structure, but also the adsorption device 200 and the culture bottle 100 are directly nested and connected to each other, reducing the occurrence of the problem of contamination of the sample to be cultured and the culture medium by external impurities during the process of the sample to be cultured flowing into the culture bottle 100.

[0066] In a specific embodiment, a conical portion is provided in the inner cavity of the adsorption device 200. The conical portion is a cavity structure with openings at both ends, and the inner cavity of the conical portion communicates with the inner cavity of the adsorption device 200. The first end of the conical portion is connected to the inner wall of the adsorption device 200, so that the conical portion is fixed on the inner wall of the adsorption device 200. The diameter of the second end of the conical portion is smaller than the diameter of the bottle mouth of the culture bottle 100, so that the second end of the conical portion can extend into the culture bottle 100 or into the inner cavity of the culture bottle 100 through the bottle mouth of the culture bottle 100. At the same time, the diameter of the first end of the conical portion is larger than the diameter of the bottle mouth of the culture bottle 100, so that the outer wall of the conical portion can be clamped on the bottle mouth of the culture bottle 100 to achieve relative fixation between the conical portion and the culture bottle 100. At this time, the adsorption material 300 is located between the conical portion and the inlet. The to-be-cultured sample after being adsorbed by the adsorption material 300 flows into the culture bottle 100 through the conical portion. When the bottle mouth extension portion 110 is embedded in the inner cavity of the adsorption device 200, the conical portion can be directly clamped on the bottle mouth of the culture bottle 100, further improving the connection stability between the culture bottle 100 and the adsorption device 200.

[0067] As Figure 3 shown, in one embodiment, one end of the adsorption device 200 provided with an outlet extends into the inner cavity of the culture bottle 100, opposite to the culture area. A clearance is provided between the outlet of the adsorption device 200 and the culture medium in the culture bottle 100 to separate the adsorption material 300 and the culture medium. One end of the adsorption device 200 provided with an inlet extends out of the culture bottle 100 to facilitate injecting the to-be-cultured sample into the adsorption device 200. In addition, the adsorption device 200 and the culture bottle 100 can be integrally processed, saving the assembly work link between the adsorption device 200 and the culture bottle 100 and further improving the efficiency of the culture work.

[0068] In one embodiment, as Figure 4 shown, a blocking layer 120 is provided in the inner cavity of the culture bottle 100. The blocking layer 120 extends along the radial direction of the culture bottle 100 and is connected to the inner wall of the culture bottle 100, dividing the inner cavity of the culture bottle 100 into upper and lower chambers. The lower chamber is used as the culture area, and the upper chamber forms an accommodation space. At the same time, a plurality of through holes are arranged at intervals in the blocking layer 120, and the through holes communicate the culture area and the accommodation space. Before adding the to-be-cultured sample to the culture medium, first place the adsorption material on the blocking layer 120 in the inner cavity of the culture bottle 100, and then pour in the to-be-cultured sample. The to-be-cultured sample adsorbed by the adsorption material 300 flows into the lower culture medium through the through holes in the blocking layer 120. A clearance is left between the blocking layer 120 and the culture area in the culture bottle 100 to separate the adsorption material 300 from the culture medium and avoid specific adsorption of the culture substances in the culture medium by the adsorption material 300.

[0069] As Figure 5As shown, in one embodiment, a partition layer 130 is provided in the inner cavity of the culture bottle 100. The first end of the partition layer 130 is connected to the bottom of the culture bottle 100, and the second end of the partition layer 130 extends towards the mouth of the culture bottle 100, such that the partition layer 130 divides the inner cavity of the culture bottle 100 into a first chamber 140 and a second chamber 150. An adsorption material 300 is placed in the first chamber 140, and the culture medium is placed in the second chamber 150, such that the first chamber 140 forms an adsorption device 200, and the second chamber 150 serves as a culture area. At the same time, the first chamber 140 is located on one side of the mouth of the culture bottle 100. When adding a sample to be cultured through the mouth of the culture bottle 100, the sample to be cultured will directly flow into the first chamber 140 and be adsorbed by the adsorption material 300. At this time, the partition layer 130 separates the adsorption material 300 from the culture medium in the second chamber 150. A communication port is provided between the second end of the partition layer 130 and the inner wall of the culture bottle 100, and the first chamber 140 and the second chamber 150 can be connected through the communication port (for example, tilting the culture bottle 100 in Figure 5 a certain angle to the right), so that the sample to be cultured that has been adsorbed in the first chamber 140 can be poured into the second chamber 150 through the communication port and merged with the culture medium.

[0070] In addition, the inner cavity of the culture bottle 100 has a certain degree of vacuum, and the vacuum value is maintained within a preset range to facilitate the smooth collection of the sample to be cultured. In addition, according to actual needs (such as aerobic or anaerobic culture), the inner cavity of the culture bottle 100 can be set with a suitable gas environment and the composition of the culture medium can be adjusted.

[0071] In one embodiment, as Figure 7 and Figure 8 shown, the adsorption device 200 and the culture area can be arranged in different regions within the same container (such as the culture bottle 100). The adsorption device 200 includes a partition layer 160 in the form of a membrane structure or a cage-like structure. The partition layer 160 and the inner wall of the container jointly enclose the above-mentioned accommodation space. The adsorption material 300 is covered and fixed by the partition layer 160 and fixed on the inner wall of the container, and the partition layer 160 is provided with an avoidance structure (exemplary avoidance structures can be holes, strip-shaped gaps, etc.) through which the sample to be cultured can pass. The avoidance structure only allows the sample to be cultured to pass through, while the adsorption material 300 cannot pass through. The container can be flipped, and when the container is rotated to the first state, the accommodation space is arranged above the culture area, and when the container is rotated to the second state, the accommodation space is arranged below the culture area.

[0072] In use, a sample to be cultured containing a culture medium is added to the container in the second state described above, and the adsorption material 300 is brought into full contact and mixed with the sample to be cultured, so as to adsorb and cultivate the sample to be cultured through the adsorption material 300. After culturing for a preset time, the container is turned over so that the container is in the first state, and the sample to be cultured is separated from the adsorption material 300 under the action of gravity and flows to a position (culture area) where it will not come into contact with the adsorption material 300, and then the sample can be continuously cultured at an appropriate temperature.

[0073] Combined Figure 7 with Figure 8 , for example, the container can be turned over 180° up and down, and both ends of the container serve as the adsorption device 200 and the culture area respectively. Figure 7 Corresponding to the second state of the container, at this time, a sample to be cultured can be added to the container for adsorption culture. After adsorbing for a preset time, the container is turned over 180° up and down to Figure 8 the state of ( Figure 8 corresponding to the first state of the container), the sample to be cultured in the container flows into the culture area at the other end of the container under the action of gravity, while the position of the adsorption material 300 remains stationary and is separated from the sample to be cultured. At this time, the culture of the sample to be cultured in the culture area can be free from the influence of the adsorption material 300.

[0074] In a specific test example, the bloodstream infection microorganisms in Table 1 below were cultured and divided into six groups for testing. Among them, the first group did not use the above-mentioned culture method and equipment to adsorb and treat the antibiotics in the sample to be cultured, while the other five groups treated the sample to be cultured with different adsorption times through the culture method and equipment provided by the present utility model.

[0075] Table 1

[0076] Strain Strain number Pseudomonas aeruginosa ATCC27853 Alcaligenes faecalis ATCC8750 Streptococcus pneumoniae ATCC6305 Haemophilus influenzae ATCC19418 Neisseria meningitidis ATCC13090 Streptococcus pyogenes ATCC19615 Staphylococcus aureus ATCC25923 Candida albicans ATCC18804 Escherichia coli ATCC25922

[0077] Specific steps:

[0078] 1. Take the dry powder of the strain, reconstitute it with sterile normal saline, inoculate the plate and place it in a constant temperature incubator at 35°C - 37°C for culture.

[0079] 2. Pick the colonies on the plate cultured for 18h - 24h and prepare them into a 0.5 McFarland bacterial suspension, and dilute it serially to 5 cfu / ml - 30 cfu / ml.

[0080] 3. Sterilize the culture equipment and the macroporous adsorption resin HPD100B (adsorption material 300), and in accordance with the ratio of the adsorption material 300 to the sample to be cultured of 1:1 (mass ratio), add 10 ml of sterile fresh normal human blood, 1 ml of the diluted bacterial suspension of 5 cfu / ml - 30 cfu / ml and the antibiotic solution to the adsorption device 200 in sequence.

[0081] 4. Detection was performed using the on-machine microbial culture monitor BC60, the detection time was recorded, and the test results are shown in Table 2 below.

[0082] Table 2

[0083]

[0084] From the test data in Table 2, it can be seen that compared with the first group treated by the prior art (continuously maintaining the mixture of the adsorption material 300 with the sample to be cultured and the culture medium), the 1-minute treatment group was not sufficient to eliminate the influence of antibiotics. However, for the groups with the treatment time extended to 5 minutes and above, the samples to be cultured were treated by the culture method and equipment provided by the present utility model, which avoided the non-specific adsorption of the culture substances in the culture medium by the adsorption material 300, ensured the culture performance of the culture medium, significantly improved the detection rate of microbial detection, and shortened the positive culture time.

[0085] In another specific test example, common industrial contaminated microorganisms in Table 3 below were cultured and divided into six groups for testing. Among them, the first group did not use the above-mentioned culture method and equipment to adsorb and treat the antibiotics in the samples to be cultured, while the other five groups adsorbed and treated the samples to be cultured by the culture method and equipment provided by the present utility model.

[0086] Table 3

[0087] Strain Strain number Staphylococcus aureus CMMCC(B)26003 Pseudomonas aeruginosa CMCC(B)10104 Bacillus subtilis CMCC(B)63501 Candida albicans CMCC(F)98001 Aspergillus niger CMCC(F)98003

[0088] Specific steps:

[0089] 1. The dry strain powder was reconstituted with sterile normal saline, inoculated on a plate, and placed in a constant temperature incubator at 35°C - 37°C for culture.

[0090] 2. Colonies on the plate cultured for 18h - 48h were picked and prepared into a 0.5 McFarland bacterial suspension, which was serially diluted to 5 cfu / ml - 30 cfu / ml.

[0091] 3. The culture equipment and activated carbon (adsorption material 300) were sterilized. According to the ratio of the adsorption material 300 to the sample to be cultured of 1:2, then 10 ml of cell culture medium, 1 ml of the diluted bacterial suspension of 5 cfu / ml - 30 cfu / ml, and the antibiotic solution were sequentially added to the adsorption device 200, and the preset adsorption time was set to 15 minutes.

[0092] 4. Detection was performed using the on-machine microbial culture monitor BC60, the detection time was recorded, and the test results are shown in Table 4 below.

[0093] Table 4

[0094]

[0095] It can be seen from the test data in Table 4 that, compared with the first group processed by the prior art (continuously maintaining the mixture of the adsorption material 300, the sample to be cultured and the culture medium), the 1-minute treatment group is not sufficient to eliminate the influence of antibiotics. However, for the groups with a treatment time extended to 5 minutes and above, by using the culture method and equipment provided by the present utility model to process the sample to be cultured, the non-specific adsorption of the culture substances in the culture medium by the adsorption material 300 is avoided, the culture performance of the culture medium is ensured, the detection rate of microbial detection is significantly improved, and the positive culture time is shortened. It should be noted that each embodiment in this specification is described in a progressive manner, and the key point of each embodiment is the difference from other embodiments. The same or similar parts among the embodiments can be referred to each other.

[0096] It should be noted that each embodiment in this specification is described in a progressive manner, and the key point of each embodiment is the difference from other embodiments. The same or similar parts among the embodiments can be referred to each other.

[0097] In this article, specific examples are used to elaborate on the principle and implementation manner of the present utility model. The description of the above embodiments is only used to help understand the core idea of the present utility model. It should be pointed out that for those of ordinary skill in the art in this technical field, without departing from the principle of the present utility model, several improvements and modifications can still be made to the present utility model, and these improvements and modifications also fall within the protection scope of the claims of the present utility model.

Claims

1. A culture device, characterized in that: It comprises an adsorption device (200) and a culture area for containing a culture medium, wherein the adsorption device (200) has a containing space for placing an adsorption material (300), and the culture area is used to store a sample to be cultured after being adsorbed by the adsorption material (300) for a preset time.

2. The culture device according to claim 1, characterized in that: A connecting channel is provided between the adsorption device (200) and the culture area.

3. The culture device according to claim 1 or 2, characterized in that: There is a pressure difference between the adsorption device (200) and the culture area; and / or, The adsorption device (200) is provided with an inlet and an outlet both connected to the containing space; a sample containing area for containing samples to be cultured is provided between the inlet and the containing space; the samples to be cultured are used to pass through the sample containing area, the containing space and the inlet in sequence.

4. The culture device according to claim 3, characterized in that: The adsorption device (200) is further provided with a sealing cover (400), and the sealing cover (400) is used to adjust the sealing area of ​​the inlet to control the speed at which the sample to be cultured flows out of the inlet; and / or, The adsorption device (200) is further provided with a flow guide tube (210), and the flow guide tube (210) is connected to the outflow port, so that the sample to be cultured flowing out of the outflow port flows into the culture area through the flow guide tube (210).

5. The culture device according to claim 3, characterized in that: The culture area is arranged in the cavity of the culture bottle (100), and there is an avoidance gap between the adsorption material (300) and the culture area.

6. The culture device according to claim 5, characterized in that: The culture bottle (100) is provided with a bottle mouth extension portion (110), and the bottle mouth of the culture bottle (100) is connected to the culture area through the bottle mouth extension portion (110). The adsorption device (200) is provided with a mounting opening, so that the bottle mouth extension portion (110) extends into the inner cavity of the adsorption device (200) through the mounting opening, and the edge of the mounting opening is clamped on the outer wall of the bottle mouth extension portion (110), and the adsorption material (300) is located between the inlet and the bottle mouth.

7. The culture device according to claim 5, characterized in that: The adsorption device (200) is provided with one end of the outflow port extending into the inner cavity of the culture bottle (100), and is opposite to the culture area, and has the avoidance gap with the culture area. The adsorption device (200) is provided with one end of the inflow port extending out of the inner cavity of the culture bottle (100).

8. The culture device according to claim 5, characterized in that: The adsorption device (200) and the culture bottle (100) are an integrated structure.

9. The culture device according to claim 5, characterized in that: A barrier layer (120) is arranged in the inner cavity of the culture bottle (100); the barrier layer (120) is connected to the inner wall of the culture bottle (100) as the adsorption device (200), and divides the culture bottle (100) into two upper and lower chambers, the lower chamber being the culture area, and the upper chamber forming the accommodation space; the barrier layer (120) is provided with a plurality of through holes, and the through holes connect the culture area and the accommodation space.

10. The culture device according to claim 5, characterized in that: A partition layer (130) is provided in the inner cavity of the culture bottle (100), and the partition layer (130) divides the inner cavity of the culture bottle (100) into a first chamber (140) and a second chamber (150); the first chamber (140) contains the adsorption material (300) to serve as the adsorption device (200), and the first chamber (140) corresponds to the bottle mouth of the culture bottle (100); the second chamber (150) avoids the bottle mouth and is used to contain culture medium to serve as the culture area; The first end of the partition layer (130) is connected to the bottom of the culture bottle (100), the second end of the partition layer (130) extends toward the bottle mouth of the culture bottle (100), and a connecting port is provided between the second end of the partition layer (130) and the inner wall of the culture bottle (100), and the first chamber (140) and the second chamber (150) are connected through the connecting port.

11. The culture device according to claim 1, characterized in that: The adsorption device (200) and the culture area are arranged in different areas in the same container, the adsorption device (200) comprises an isolation layer (160) which is enclosed together with the inner wall of the container to form the accommodation space, the isolation layer (160) is provided with an avoidance structure for the sample to be cultured to pass through, and the avoidance structure separates the adsorption material (300) and the culture area; The container is flippable, and when the container is rotated to a first state, the accommodating space is arranged above the culture area, and when the container is rotated to a second state, the accommodating space is arranged below the culture area.

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