Oscillation detection device

Abstract

The utility model belongs to the technical field of detection equipment, and discloses an oscillation detection device which comprises a shading base, an oscillation assembly, a sample disc and a detection assembly. The oscillation assembly is located in the shading cavity, the sample disc is arranged on the oscillation assembly, and a plurality of sample holes are formed in the sample disc; the detection assembly is located in the shading cavity and located above the oscillation assembly, the detection assembly comprises a light source and a photoelectric plate, the light source is configured to emit light with a specific wavelength to the sample disc on the oscillation assembly, and the photoelectric plate is configured to detect the light absorption value of the to-be-detected sample. The oscillation detection device can realize integrated detection of lipase and is high in detection efficiency.

Description

Technical Field

[0001] The utility model relates to the technical field of detection equipment, in particular to an oscillation detection device. Background Art

[0002] The activity of lipase has a great influence on the quality of liquid foods such as milk. Therefore, the determination of lipase activity has become a very important link in food detection. During the determination of lipase activity, multiple processes such as mixing and detection need to be introduced. Usually, mixing is realized by an oscillator. After mixing, the tester needs to transfer the sample tray containing the sample to be tested from the oscillator to the detector, resulting in low detection efficiency. Summary of the Utility Model

[0003] The purpose of the utility model is to provide an oscillation detection device, which can integrate the oscillation function and the detection function into one, and has a high detection effect on lipase.

[0004] To achieve the above object, the utility model adopts the following technical solutions:

[0005] The oscillation detection device includes a light-shielding base, an oscillation component, a sample tray and a detection component. A light-shielding cavity is arranged in the light-shielding base; the oscillation component is located in the light-shielding cavity, the sample tray is placed on the oscillation component, and a plurality of sample holes are arranged on the sample tray to be able to hold the sample to be tested; the oscillation component is configured to drive the sample tray to vibrate reciprocally; the detection component is located in the light-shielding cavity and above the oscillation component. The detection component includes a light source and a photoelectric plate. The light source is configured to emit light with a specific wavelength to the sample tray on the oscillation component, and the photoelectric plate is configured to detect the light absorption value of the sample to be tested.

[0006] In one embodiment, an opening communicating with the light-shielding cavity is arranged on one side of the light-shielding base along a first direction. The oscillation component includes a tray and an oscillation power component. The oscillation power component is configured to drive the tray to move reciprocally along the first direction, and at least part of the tray can extend out of the opening under the action of the oscillation power component.

[0007] In one embodiment, the tray is arranged in a drawer-like structure.

[0008] In one embodiment, the oscillation component further includes a pulley and a belt. The oscillation power component is a stepping motor. The belt is wound around the two pulleys, and the stepping motor is connected to at least one of the pulleys.

[0009] In one embodiment, the oscillation detection device further includes a guiding component. The guiding component includes a first guiding structure and a second guiding structure that cooperate with each other. The first guiding structure is arranged on the tray, and the second guiding structure is arranged in the light-shielding cavity.

[0010] In one embodiment, the first guiding structure is a guiding hole penetrating through the pallet along the first direction, and the second guiding structure is a guiding rod inserted into the guiding hole. The pallet and the light-shielding base are slidably engaged through the guiding hole and the guiding rod.

[0011] In one embodiment, the sample tray includes a heat-insulating layer, a temperature-regulating layer, and a heat-transfer layer arranged in sequence from bottom to top, and the sample holes are formed in the heat-transfer layer.

[0012] In one embodiment, the temperature-regulating layer includes an electric heating wire, a PCB circuit board, and a temperature sensor, and the PCB circuit board is electrically connected to both the electric heating wire and the temperature sensor.

[0013] In one embodiment, the temperature-regulating layer further includes a semiconductor refrigeration chip electrically connected to the PCB circuit board.

[0014] In one embodiment, the oscillation detection device further includes a display screen and a controller. The display screen is arranged on the light-shielding base, and the controller is communicatively connected to the display screen, the detection component, and the oscillation component.

[0015] Advantages of the present utility model: In the oscillation detection device of the present utility model, the oscillation component and the detection component are integrated inside the light-shielding base, which can integrally realize the oscillation operation and the detection operation in the lipase detection process, greatly improving the detection efficiency and the convenience of the work process. Moreover, due to the omission of the transfer process, the detection accuracy and reliability are also greatly improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 is a schematic structural diagram of the oscillation detection device in an embodiment of the present utility model.

[0017] In the figure:

[0018] 1. Light-shielding base; 1a. Light-shielding cavity; 2. Sample tray; 3. Oscillation component; 31. Pallet; 32. Oscillation power member; 33. Pulley; 34. Belt; 4. Detection component; 41. Photoelectric board; 42. Light source; 5. Display screen; 6. Controller; 7. Guiding component; 71. First guiding structure; 72. Second guiding structure. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0019] The following further elaborates on the present utility model in conjunction with the drawings and embodiments. It can be understood that the specific embodiments described herein are merely for explaining the present utility model and do not limit the present utility model. Additionally, it should be noted that for the sake of description, only the parts related to the present utility model are shown in the drawings rather than all the structures.

[0020] In the description of the present utility model, unless otherwise clearly defined and limited, the terms "connected", "connected to", and "fixed" shall be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or integrated; it may be a mechanical connection or an electrical connection; it may be directly connected or indirectly connected through an intermediate medium, and it may be the communication inside two elements or the interaction relationship between two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0021] In the present utility model, unless otherwise clearly defined and limited, the first feature being "above" or "below" the second feature may include the direct contact between the first and second features, or may include the situation where the first and second features are not in direct contact but in contact through other features between them. Moreover, the first feature being "above", "over", and "on" the second feature includes that the first feature is directly above and obliquely above the second feature, or merely indicates that the horizontal height of the first feature is higher than that of the second feature. The first feature being "below", "under", and "beneath" the second feature includes that the first feature is directly below and obliquely below the second feature, or merely indicates that the horizontal height of the first feature is lower than that of the second feature.

[0022] In the description of this embodiment, the orientation or positional relationships such as "above", "below", "right", etc. are based on the orientation or positional relationships shown in the drawings, and are only for the convenience of description and simplifying the operation, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as a limitation to the present utility model. In addition, the terms "first" and "second" are only used for distinction in description and have no special meanings.

[0023] p-Nitrophenyl ester is one of the most widely used substrates in the determination of lipase hydrolysis activity. Under the action of p-nitrophenyl ester, lipase hydrolyzes to produce p-nitrophenol, which shows yellow in an alkaline environment, has an absorbance value at a wavelength of 410 nm, and has high sensitivity. On this basis, an oscillation detection device is proposed in the embodiment of the present utility model, which can be used to detect lipase activity.

[0024] Reference Figure 1As shown in the figure, the oscillation detection device includes a light-shielding base 1, a sample tray 2, a detection component 4, and an oscillation component 3. A light-shielding cavity 1a is provided inside the light-shielding base 1. The detection component 4, the oscillation component 3, and the sample tray 2 are all located inside the light-shielding cavity 1a. The sample tray 2 is placed on the oscillation component 3, and a plurality of sample holes are provided thereon for holding samples to be tested. The oscillation component 3 is configured to drive the sample tray 2 to vibrate reciprocally. The detection component 4 is located above the oscillation component 3 and includes a light source 42 and a photoelectric plate 41. The light source 42 is provided on the photoelectric plate 41 and can emit light of a specific wavelength to the sample tray 2 located on the oscillation component 3. The photoelectric plate 41 is used to detect the absorbance value of the sample to be tested.

[0025] The above oscillation detection component 4 places the oscillation component 3 and the detection component 4 inside the dark light-shielding cavity 1a, realizes the vibration of the sample tray 2 placed inside the light-shielding cavity 1a through the oscillation component 3, and realizes the detection of the absorbance value of p-nitrophenol, the product of lipase, through the detection component 4, thereby obtaining the lipase activity, achieving the integration of the oscillation operation and the detection operation, reducing manual intervention, and having high detection efficiency.

[0026] To facilitate placing the sample to be tested on the sample tray 2, an opening communicating with the light-shielding cavity 1a is further provided on one side of the light-shielding base 1 along the first direction. The oscillation component 3 includes a support plate 31 and an oscillation power member 32. The support plate 31 is connected to the oscillation power member 32. The oscillation power member 32 is configured to drive the support plate 31 to reciprocally move along the first direction ( Figure 1 X direction in the figure), thereby realizing the vibration of the sample tray 2. At the same time, the support plate 31 can also at least partially extend out of the opening under the action of the oscillation power member 32 to facilitate placing the sample to be tested on the sample tray 2.

[0027] Optionally, the oscillation power member 32 adopts a stepping motor. The vibration component further includes a belt 34 and belt pulleys 33. The belt 34 is wound between two belt pulleys 33 spaced along the first direction. The stepping motor is connected to at least one belt pulley 33 for driving the belt pulley 33 to rotate. The support plate 31 is connected to the belt 34 and can move together with the belt 34, thereby realizing the oscillation of the support plate 31 driving the sample tray 2 or extending out of and retracting into the light-shielding cavity 1a.

[0028] To prevent the existence of the opening from affecting the light-shielding effect of the light-shielding cavity 1a, the support plate 31 is set as a drawer-like structure. After the support plate 31 enters the light-shielding cavity 1a, the side wall on one side thereof can block the opening.

[0029] Alternatively, a light-shielding door is further provided on the light-shielding base 1. The light-shielding door is hinged at the inlet of the light-shielding base 1. Optionally, a limiting step is provided on the light-shielding base 1. The light-shielding door can abut against the limiting step, thereby restricting the light-shielding door from flipping inward into the light-shielding cavity 1a and affecting the light-shielding effect.

[0030] In other embodiments, the oscillating power member 32 may also be an electric telescopic rod, and the movement of the tray 31 is realized by the telescoping of the electric telescopic rod.

[0031] To further improve the stability of the sample tray 2 during movement, the oscillation detection device further includes a guiding assembly 7. The guiding assembly 7 includes a first guiding structure 71 and a second guiding structure 72 that cooperate with each other. The first guiding structure 71 is disposed on the tray 31, and the second guiding structure 72 is disposed on the light-shielding base 1 and located within the light-shielding cavity 1a. Under the cooperation of the first guiding structure 71 and the second guiding structure 72, the tray 31 can drive the sample tray 2 to stably move in the first direction.

[0032] In one embodiment, the first guiding structure 71 is a guiding hole that penetrates the tray 31 in the first direction, and the second guiding structure is a guiding rod inserted into the guiding hole. The tray 31 and the light-shielding base 1 are slidably engaged through the guiding hole and the guiding rod.

[0033] In other embodiments, the first guiding structure 71 is a pulley, and the pulley is rotatably disposed on the tray 31. The second guiding structure 72 is a slide rail, and the slide rail is disposed along the first direction on the inner wall of the light-shielding base 1, that is, the wall of the light-shielding cavity 1a. The pulley is slidably engaged with the slide rail. Specifically, two sets of guiding assemblies 7 are provided, and the two sets of pulleys are respectively disposed on opposite sides of the tray 31 along the second direction. The second direction is perpendicular to the first direction, and the second direction is the Y direction in the figure.

[0034] In one embodiment, the sample tray 2 includes a heat-insulating layer, a temperature-regulating layer, and a heat-transfer layer sequentially arranged from bottom to top. The heat-transfer layer is made of a metal material and can conduct heat. The sample holes are opened on the heat-transfer layer. To adapt to different sample detections, the heat-transfer layer and the temperature-regulating layer are detachably connected by means including but not limited to snap connection and bolt connection. The temperature-regulating layer is used to adjust the temperature to meet the constant-temperature requirement during the lipase detection process, and the heat-insulating layer is used to isolate the temperature-regulating layer and the tray 31 to prevent the tray 31 from being damaged.

[0035] Optionally, the temperature-regulating layer includes an electric heating wire, a PCB circuit board, and a temperature sensor. The PCB circuit board is electrically connected to both the electric heating wire and the temperature sensor. The temperature sensor is used to monitor the temperature of the electric heating wire, and the PCB circuit board can control the electric heating wire according to the temperature feedback by the temperature sensor to keep the temperature at a preset temperature. For example, if the required temperature for the constant-temperature oscillation incubation of lipase is 40 ± 1 °C, the PCB circuit board controls the electric heating wire to keep its temperature within the range of 40 ± 1 °C.

[0036] To achieve heat dissipation and prevent heat from accumulating in the light-shielding base 1, heat dissipation holes are further provided at the bottom of the light-shielding base 1.

[0037] In other embodiments, the temperature adjustment layer further includes a thermoelectric cooler electrically connected to the PCB circuit board. The thermoelectric cooler can be used for refrigeration, and the temperature sensor can also be used to detect the temperature of the thermoelectric cooler.

[0038] In one embodiment, the oscillation detection device further includes a display screen 5 and a controller 6. The display screen 5 is disposed on the light-shielding base 1; the controller 6 is communicatively connected to the display screen 5, the detection component 4, the PCB circuit board of the sample tray 2, and the oscillation power member 32 in the oscillation component 3. The preset temperature, oscillation time, and oscillation frequency can be set through the controller 6 to meet different oscillation incubation conditions, and the display screen 5 can display the results detected by the detection component 4.

[0039] The process of detecting lipase using the above oscillation detection device is briefly described below:

[0040] During use, take 180 μL of the diluted sample to be measured and place it in the sample well of the sample tray 2, then add 20 μL of p-nitrophenyl ester. Transfer the sample tray 2 to the pallet 31. Under the action of the oscillation power member 32, the pallet 31 transfers the sample tray 2 into the light-shielding chamber 1a. Measure the initial absorbance value of the sample to be measured at a wavelength of 405 nm, denoted as A0. Then oscillate the sample tray 2 and incubate it at 40 ± 1 °C and 150 r / min for 30 min. Re-measure the absorbance value of the sample to be measured at a wavelength of 405 nm, denoted as A1. According to the standard curve, calculate the concentration of p-nitrophenol corresponding to the value of A1 - A0.

[0041] Then according to the calculation formula: enzyme activity = [Δc / (0.01 × t)] × D

[0042] In the formula:

[0043] △c is the concentration change caused by the change in absorbance value during the reaction time, which is calculated by substituting A1 - A0 (negative value

[0044] calculated as 0) into the standard curve;

[0045] t is the reaction time (min);

[0046] D is the dilution factor, that is, the multiple of the total enzyme solution extracted to the enzyme solution in the reaction system.

[0047] It can be understood that the sample to be measured can be directly placed in the sample well, or the reagent tube containing the sample to be measured can be placed in the sample well to reduce the time for cleaning the sample tray 2 and improve the detection efficiency.

[0048] The above-mentioned oscillation detection device can be used for the detection of lipase, realize accurate temperature control and stable oscillation of lipase detection, ensure that the detection is carried out under appropriate conditions, and improve the accuracy and reliability of the detection. Through the clever combination with spectral detection technology, it can efficiently obtain detection data and quickly output results, greatly improving the detection efficiency and the convenience of the workflow.

[0049] Obviously, the above embodiments of the present invention are merely examples for the purpose of clearly illustrating the present invention, and are not intended to limit the implementation methods of the present invention. For those skilled in the art, various obvious changes, readjustments and substitutions can be made without departing from the scope of protection of the present invention. It is not necessary and impossible to list all implementation methods here. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention shall be included in the scope of protection of the claims of the present invention.

Claims

1. An oscillation detection device, characterized in that: include: A light-shielding base (1), wherein a light-shielding cavity (1a) is provided inside the light-shielding base (1); An oscillating component (3) is located in the light shielding cavity (1a). A sample plate (2) is placed on the oscillating component (3); a plurality of sample holes are provided on the sample plate (2) so as to accommodate samples to be tested; the oscillating component (3) is configured to drive the sample plate (2) to vibrate back and forth; A detection component (4) is located in the light-shielding cavity (1a) and above the oscillation component (3). The detection component (4) comprises a light source (42) and a photoelectric panel (41). The light source (42) is configured to emit light of a specific wavelength to the sample disk (2) on the oscillation component (3), and the photoelectric panel (41) is configured to detect the absorbance value of the sample to be tested.

2. The oscillation detection device according to claim 1, characterized in that: The shading base (1) is provided with an opening connected to the shading cavity (1a) on one side along the first direction; the oscillating assembly (3) comprises a support plate (31) and an oscillating power member (32); the oscillating power member (32) is configured to drive the support plate (31) to move back and forth along the first direction; and the support plate (31) can at least partially extend out of the opening under the action of the oscillating power member (32).

3. The oscillation detection device according to claim 2, characterized in that: The support plate (31) is configured as a drawer-like structure.

4. The oscillation detection device according to claim 2, characterized in that: The oscillation assembly (3) further comprises a pulley (33) and a belt (34); the oscillation power member (32) is a stepping motor; the belt (34) is wound around two of the belts (34); and the stepping motor is connected to at least one of the pulleys (33).

5. The oscillation detection device according to claim 2, characterized in that: The oscillation detection device further comprises a guide assembly (7), wherein the guide assembly (7) comprises a first guide structure (71) and a second guide structure (72) which cooperate with each other, wherein the first guide structure (71) is arranged on the support plate (31), and the second guide structure (72) is arranged in the light shielding cavity (1a).

6. The oscillation detection device according to claim 5, characterized in that: The first guide structure (71) is a guide hole that penetrates the support plate (31) along the first direction, and the second guide structure (72) is a guide rod that passes through the guide hole. The support plate (31) and the light-shielding base (1) are slidably matched through the guide hole and the guide rod.

7. The oscillation detection device according to claim 1, characterized in that: The sample plate (2) comprises a heat insulation layer, a temperature adjustment layer and a heat transfer layer which are arranged in sequence from bottom to top, and the sample hole is opened on the heat transfer layer.

8. The oscillation detection device according to claim 7, characterized in that: The temperature adjustment layer includes an electric heating wire, a PCB circuit board and a temperature sensor, and the PCB circuit board is electrically connected to the electric heating wire and the temperature sensor.

9. The oscillation detection device according to claim 8, characterized in that: The temperature adjustment layer also includes a semiconductor cooling sheet electrically connected to the PCB circuit board.

10. The oscillation detection device according to claim 1, characterized in that: The oscillation detection device further comprises a display screen (5) and a controller (6); the display screen (5) is arranged on the light-shielding base (1); and the controller (6) is communicatively connected with the display screen (5), the detection component (4) and the oscillation component (3).

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