High-throughput grid staining instrument

Abstract

The utility model discloses a high-flux grid dyeing instrument, which comprises a grid storage plate and a sealing element, the top surface of the grid storage plate is provided with a plurality of communicated storage grooves, the grid storage plate is also provided with a liquid / gas inlet and a liquid / gas outlet which are communicated with the storage grooves, and the sealing element is arranged on the surface of the grid storage plate; the storage grooves are used for storing the carrying net, the sealing pieces cover notches of the storage grooves to enable the connected storage grooves to be relatively closed, staining liquid is discharged into the liquid / gas inlet so that the storage grooves can be filled with the staining liquid to dye the carrying net and the ultrathin section of the carrying net, and the staining liquid is discharged from the liquid / gas outlet after staining is completed. And meanwhile, cleaning and drying operations are realized through a liquid / gas inlet and a liquid / gas outlet. By adopting the arrangement, the carrying net can be stored in the carrying net storage plate at the same time, the ultra-thin section on the carrying net can be dyed, and the device has the effects of being easy and convenient to operate and saving cost.

Description

Technical Field

[0001] The utility model belongs to the technical field of the structure of a staining instrument and staining technology, and particularly relates to a high-throughput grid staining instrument. Background Art

[0002] In the prior art, when observing the internal ultrastructure of biological sample cells by using a transmission electron microscope, it is necessary to use an ultra-thin section preparation technique to prepare the sample into an ultra-thin section with a thickness within 100 nm, and carry it on a grid with a diameter of about 3 mm, an inner diameter of about 2.6 nm, a ring width of about 0.2 mm, and a thickness of about 0.02 mm. The grid is clamped and moved by tweezers at the ring width position to stain the ultra-thin section carried by the grid. After removing the grid, microscopic imaging observation of the ultra-thin section is carried out under the transmission electron microscope. In the staining technology, usually two kinds of staining solutions are used for two successive stainings, and after each staining, rinsing and air-drying operations are required.

[0003] In practice, the grids are usually stored in a grid box uniformly, and when staining is required, the grids are clamped by tweezers and placed into a corresponding staining device, and the grids are moved to achieve staining; sometimes, for the convenience of operation, the entire grid rack is also considered to be immersed in a corresponding staining device to achieve collective and unified staining. However, the grid storage and staining functions are independently in two different structures, and the two structures need to be assembled or coordinated during use, resulting in inconvenient operation and increased processing and production costs. Content of the Utility Model

[0004] In order to overcome the deficiencies of the prior art, the purpose of the utility model is to provide a high-throughput grid staining instrument, which can store grids in a grid rack and simultaneously stain the ultra-thin sections on the grids, and has the functions of simple operation and cost saving.

[0005] The purpose of the utility model is achieved by the following technical solutions:

[0006] A high-throughput grid staining instrument includes a grid storage board and a seal. A plurality of interconnected storage grooves are formed on the top surface of the grid storage board. The grid storage board is also provided with a liquid / gas inlet and a liquid / gas outlet both connected to the storage grooves. The seal is arranged on the surface of the grid storage board; the storage grooves are used for storing grids, and the seal covers the openings of a plurality of the storage grooves to make a plurality of interconnected storage grooves relatively airtight. By discharging a staining solution into the liquid / gas inlet, a plurality of the storage grooves are filled with the staining solution to stain the grids and their ultra-thin sections. After the staining is completed, the staining solution is discharged from the liquid / gas outlet, and at the same time, cleaning and drying operations are realized through the liquid / gas inlet and the liquid / gas outlet.

[0007] Further, the seal is composed of an upper clamping plate and a lower clamping plate. The upper clamping plate is hinged to the lower clamping plate through a hinge. A locking member is provided on the side of the upper clamping plate away from the hinge. The lower clamping plate is provided with a receiving groove, and the carrier mesh storage plate is arranged in the receiving groove. The liquid / gas inlet and outlet of the carrier mesh storage plate extend outside the lower clamping plate. After the upper clamping plate is closed, the locking member is detachably connected to the lower clamping plate, and the upper clamping plate covers the openings of a number of the storage grooves.

[0008] Further, the upper clamping plate is provided with a counterbore corresponding to the receiving groove. Sealing rubber pads are arranged on the inner bottom surfaces of the receiving groove and the counterbore. When the upper clamping plate is closed and locked by the locking member, the two sealing rubber pads clamp the carrier mesh storage plate.

[0009] Further, a locking hook is provided on the side of the lower clamping plate away from the hinge. The locking member includes a quick lock. The quick lock is arranged on the side of the upper clamping plate away from the hinge. When the upper clamping plate is closed, the quick lock is rotated to be hooked with the locking hook.

[0010] Further, a number of the storage grooves on the carrier mesh storage plate are arranged in an array. A number of connecting grooves are provided on the top surface of the carrier mesh storage plate; in a row of the storage grooves, the connecting grooves are spaced apart, and the connecting grooves connect adjacent storage grooves. A U-shaped groove is provided between adjacent storage grooves. The connecting grooves and the U-shaped groove are arranged in a staggered manner in a row of the storage grooves, so that a row of the storage grooves are connected through the connecting grooves and the U-shaped groove; connecting grooves are provided between a number of rows of the storage grooves, so that a number of the storage grooves on the carrier mesh storage plate are connected. The liquid / gas inlet and outlet are both provided on the bottom surface of the carrier mesh storage plate.

[0011] Further, it further includes a dye solution assembly, a rinsing assembly, a ventilation assembly and a waste liquid assembly. The liquid outlet end of the dye solution assembly, the liquid outlet end of the rinsing assembly and the gas outlet end of the ventilation assembly are all connected to the liquid / gas inlet, and the liquid / gas outlet is connected to the liquid inlet end of the waste liquid assembly.

[0012] Further, there are multiple dye solution assemblies, which are respectively used to provide two different dye solutions. The dye solution assembly includes a linear moving member and a dye solution syringe. The dye solution syringe has a push rod. The injection port of the dye solution syringe is connected to the liquid / gas inlet through a one-way valve. The moving end of the linear moving member is connected to the push rod.

[0013] Further, the rinsing assembly includes a water storage bottle, an air pump, and a second solenoid valve. The water storage bottle is provided with an air inlet pipe and a liquid outlet pipe. The end of the air inlet pipe is above the liquid level of the liquid in the water storage bottle, and the end of the liquid outlet pipe is close to the inner bottom surface of the liquid storage bottle. The air outlet end of the air pump is connected to the air inlet pipe through the second solenoid valve, and the top end of the liquid outlet pipe is connected to the liquid / air inlet through a check valve.

[0014] The exhaust assembly includes the air pump, a filter, and a first solenoid valve. The filter is connected to the air inlet end of the air pump, and the air outlet end of the air pump is connected to the liquid / air inlet through the first solenoid valve and a check valve.

[0015] The waste liquid assembly includes a waste liquid bottle, which is connected to the liquid / air outlet.

[0016] A staining method for a high-throughput grid dyeing instrument, using the high-throughput grid dyeing instrument as described above, includes the following steps:

[0017] 1) Storage of grids: Set the ultra-thin sections on the grids, place multiple grids in multiple storage slots for storage respectively, and cover the sealing member on the surface of the grid storage plate to cover the openings of the storage slots.

[0018] 2) Staining of grids: Pass the staining solution into the grid storage plate through the liquid / air inlet, so that each storage slot is filled with the staining solution and the ultra-thin sections on the grids are stained.

[0019] 3) Cleaning of grids: The stained staining solution is discharged from the liquid outlet, and then clear water is passed into the grid storage plate through the liquid inlet to clean each storage slot.

[0020] 4) Post-treatment after cleaning: The stained staining solution is discharged from the air outlet, and then air is passed into the grid storage plate through the air inlet respectively to dry each storage slot.

[0021] Further, in the process of cleaning and drying the grid storage plate and its storage slots, the following method is adopted: Start the air pump and the second solenoid valve to drain the clear water in the water storage bottle into the grid storage plate to rinse each storage slot, and the rinsed clear water is discharged into the waste liquid bottle from the liquid / air outlet; at the same time, start the air pump and the first solenoid valve to discharge air into the grid storage plate to dry each storage slot in the grid storage plate.

[0022] Further, in the process of passing the staining solution into the grid storage plate through the liquid / air inlet for grid staining, the following method is adopted: Start its linear moving member to drain the staining solution in the dye syringe into the grid storage plate through the liquid / air inlet for staining. When multiple stainings are required, between adjacent staining processes, the step of 3) cleaning of grids needs to be carried out.

[0023] Further, before storing the carrier grids in the step 1), the following steps are further included: Preparation before dyeing: Start the air pump and the second solenoid valve to drain the clear water in the water storage bottle into the carrier grid storage plate, and rinse each storage tank. The rinsed clear water is discharged from the liquid / gas outlet into the waste liquid bottle; then start the air pump and the first solenoid valve to discharge air into the carrier grid storage plate to dry each storage tank in the carrier grid storage plate.

[0024] The utility model has the following beneficial effects:

[0025] 1. For the high-throughput carrier grid dyeing instrument of the utility model, by providing a number of interconnected storage tanks on the carrier grid storage plate, and simultaneously providing a liquid / gas inlet and a liquid / gas outlet both connected to the storage tanks, several carrier grids can be respectively placed in several storage tanks for storage. Then, through the sealing member provided on the surface of the carrier grid storage plate to cover the openings of several storage tanks, several interconnected storage tanks are in a relatively sealed space. Thus, dyeing liquid, clear water or air can be introduced into the carrier grid storage plate through the liquid / gas inlet, so as to realize the operations of dyeing, cleaning or drying the carrier grids, and the generated waste water and waste gas can be discharged through the liquid / gas outlet. Compared with the prior art in which the carrier grid storage function and the dyeing function are realized by different structures, the utility model can integrate the carrier grid storage and dyeing functions in the same structure, which has the advantages of simple operation and cost saving.

[0026] 2. The dyeing method of the utility model mainly consists of four steps: preparation before dyeing, storage of carrier grids, dyeing of carrier grids, and post-dyeing treatment; before dyeing, the storage operation of carrier grids can be realized through multiple storage tanks of the carrier grid storage plate. During the dyeing process, dyeing liquid can be introduced into the carrier grid storage plate through the liquid / gas inlet, so that the dyeing liquid is introduced into several interconnected storage tanks to synchronously dye the carrier grids and their ultra-thin sections; at the same time, before and after dyeing, clear water and air can be introduced into the carrier grid storage plate through the liquid / gas inlet, so as to realize the operations of flushing and drying multiple storage tanks and carrier grids synchronously; therefore, it has the beneficial effect of simple and efficient operation. Description of the Drawings

[0027] Figure 1 It is a schematic diagram of the overall structure of the utility model.

[0028] Figure 2 It is a cross-sectional view schematic diagram of the lower clamping plate, the upper clamping plate and the carrier grid storage plate of the utility model.

[0029] Figure 3 It is a schematic diagram of the structure of the lower clamping plate, the upper clamping plate and the carrier grid storage plate of the utility model.

[0030] Figure 4This is a schematic diagram simulating the flow direction of the dye solution in the carrier grid storage plate of the present utility model.

[0031] In the figure:

[0032] 1. Carrier grid storage plate; 11. Storage tank; 12. Connecting groove; 13. U-shaped groove; 14. Liquid / gas inlet; 15. Liquid / gas outlet; 16. Carrier grid;

[0033] 2. Lower clamping plate; 21. Accommodating groove; 22. Lock hook; 23. Hinge; 24. Sealing gasket;

[0034] 3. Upper clamping plate; 31. Sunk groove; 32. Quick lock; 33. Handle;

[0035] 4. Dye solution assembly; 41. Linear moving part; 42. Dye solution syringe; 43. Push rod;

[0036] 5. Waste liquid assembly; 51. Waste liquid bottle; 52. Exhaust pipe;

[0037] 6. Rinsing assembly; 61. Liquid storage bottle; 62. Second solenoid valve;

[0038] 7. Ventilation assembly; 71. Air pump; 72. Filter; 73. First solenoid valve;

[0039] 8. Control circuit. Detailed implementation mode

[0040] The following further elaborates on the present utility model in conjunction with the attached drawings and specific embodiments. Terms such as "upper", "inner", "middle", "left", "right", and "one" cited in this specification are only for the convenience of description and are not used to limit the scope of implementation of the present utility model. Changes or adjustments in their relative relationships, without substantial changes in the technical content, should also be regarded as the scope of implementation of the present utility model.

[0041] Embodiment 1

[0042] A high-throughput carrier grid 16 dyeing instrument, such as Figures 1 to 4As shown in the figure, it includes a carrier mesh storage plate 1 and a seal. A plurality of interconnected storage grooves 11 are formed on the top surface of the carrier mesh storage plate 1. The carrier mesh storage plate 1 is also provided with a liquid / gas inlet 14 and a liquid / gas outlet 15 that are both connected to the storage grooves 11. The seal is arranged on the surface of the carrier mesh storage plate 1. Among them, the storage grooves 11 are used to store the carrier mesh 16 with ultra-thin sections. The seal covers the openings of a plurality of storage grooves 11 to make a plurality of interconnected storage grooves 11 relatively airtight. Therefore, by discharging the staining solution into the liquid / gas inlet 14, a plurality of storage grooves 11 are filled with the staining solution to stain the carrier mesh 16 and its ultra-thin sections. After the staining is completed, the staining solution is discharged from the liquid / gas outlet 15. At the same time, water and air are passed through the liquid / gas inlet 14 and the liquid / gas outlet 15 to achieve cleaning and drying operations.

[0043] In this embodiment, the seal adopts a fixture structure. Regarding the fixture structure: The fixture structure includes an upper clamping plate 3 and a lower clamping plate 2. The upper clamping plate 3 is hinged to the lower clamping plate 2 through a hinge. A locking member is arranged on the side of the upper clamping plate 3 away from the hinge 23. The lower clamping plate 2 is provided with a receiving groove 21. The carrier mesh storage plate 1 is inserted and fitted in the receiving groove 21. The liquid / gas inlet 14 and the liquid / gas outlet 15 of the carrier mesh storage plate 1 extend outside the lower clamping plate 2. After the upper clamping plate 3 is closed, the locking member is detachably connected to the lower clamping plate 2. The upper clamping plate 3 covers the openings of a plurality of storage grooves 11 to make a plurality of interconnected storage grooves 11 in a relatively airtight environment, which is beneficial to improving the stability of internal fluid flow.

[0044] In order to improve the sealing performance of the upper clamping plate 3 for sealing the storage grooves 11, a sunk groove 31 is formed on the upper clamping plate 3 corresponding to the receiving groove 21. Sealing rubber pads 24 are arranged on the inner bottom surfaces of the receiving groove 21 and the sunk groove 31. The sealing rubber pads 24 can be made of rubber, plastic or silica gel materials. When the upper clamping plate 3 is closed and locked by the locking member, the two sealing rubber pads 24 clamp the carrier mesh storage plate 1 and well cover the openings of a plurality of storage grooves 11.

[0045] In order for the upper clamping plate 3 to be connected to the lower clamping plate 2 by the locking member, so as to achieve the fixing effect after the upper clamping plate 3 is closed. A locking hook 22 is arranged on the side of the lower clamping plate 2 away from the hinge 23. The locking member includes a quick lock 32. The quick lock 32 is rotatably installed on the side of the upper clamping plate 3 away from the hinge 23. When the upper clamping plate 3 is closed, the quick lock 32 is rotated to hook with the locking hook 22. In this embodiment, the quick lock 32 is provided with a handle 33. The handle 33 extends to the top surface of the upper clamping plate 3, which has the advantage of facilitating the flipping of the upper clamping plate 3.

[0046] In this embodiment, in order to enable the fluid to enter the carrier grid storage plate 1 from the liquid / gas inlet 14, and after passing through a number of storage tanks 11, smoothly discharge from the liquid / gas outlet 15, so that each carrier grid 16 in each storage tank 11 can be dyed, cleaned and dried, and the working efficiency is improved. A number of storage tanks 11 on the carrier grid storage plate 1 are evenly distributed in an array, so that a number of storage tanks 11 are divided into a number of rows and a number of columns. A number of connecting grooves 12 are provided on the top surface of the carrier grid storage plate 1. In each row of storage tanks 11, a number of connecting grooves 12 are equally spaced. The connecting groove 12 connects two adjacent storage tanks 11. A U-shaped groove 13 is provided between adjacent storage tanks 11. The U-shaped groove 13 is located below the storage tank 11, and the connecting groove 12 and the U-shaped groove 13 are arranged in a staggered manner in a row of storage tanks 11, so that a row of storage tanks 11 are connected through the connecting groove 12 and the U-shaped groove 13; a connecting groove is provided between adjacent rows of storage tanks 11 in the carrier grid storage plate 1. The connecting groove is located at the head or tail of a row of storage tanks 11, so that a number of connected storage tanks 11 on the carrier grid storage plate 1 are in a continuous disk shape; wherein the liquid / gas inlet 14 and the liquid / gas outlet 15 are both provided on the bottom surface of the carrier grid storage plate 1, and the liquid / gas inlet 14 and the liquid / gas outlet 15 are respectively connected to two storage tanks 11 at both ends of the head and tail along the communication direction. Therefore, when the fluid is introduced into the carrier grid storage plate 1 through the liquid / gas inlet 14, the fluid will pass through each storage tank 11 along the flow direction and then discharge from the liquid / gas outlet 15, so that the carrier grid 16 and its ultra-thin sections in each storage tank 11 can be dyed, cleaned or dried, and the situation of the carrier grid 16 leaking dyeing can be reduced, which has the advantage of improving the working efficiency.

[0047] It should be noted that the cross-sectional contour of the storage tank 11 of the present utility model is diamond-shaped to facilitate the storage and placement of the carrier grid 16. The connecting groove 12 is provided with a flared opening to facilitate the connection between fluids. The cross-sectional size of the U-shaped groove 13 is smaller than the cross-sectional size of the storage tank 11 to reduce the situation of the stored carrier grid 16 falling into the U-shaped groove 13.

[0048] Based on this, when the carrier grid storage plate 1 and the seal of the present utility model are used separately, the dyeing solution, clean water and / or air can be introduced into the carrier grid storage plate 1 through the liquid / gas inlet 14 manually, and the waste liquid and waste gas are discharged from the liquid / gas outlet 15, so as to realize the manual operations of dyeing, cleaning and drying. Among them, the manual operation can be realized by means of an air bag or a manual air pump in the prior art.

[0049] Thus, the present utility model can realize the operations of dyeing, cleaning and drying manually or automatically. At the same time, the automatic operation can be realized through relevant structures. The following structures for realizing the automatic operation are disclosed.

[0050] The utility model further includes a dye solution assembly 4, a rinsing assembly 6, a ventilation assembly 7 and a waste liquid assembly 5. The dye solution assembly 4, the rinsing assembly 6 and the ventilation assembly 7 are all electrically connected to a control circuit 8 to control the start, stop and working duration of the dye solution assembly 4, the rinsing assembly 6 and the ventilation assembly 7 by using the control circuit 8. The liquid outlet end of the dye solution assembly 4, the liquid outlet end of the rinsing assembly 6 and the gas outlet end of the ventilation assembly 7 are all connected to the liquid / gas inlet 14 to introduce dye solution, clear water and air into the liquid / gas inlet 14. The liquid / gas outlet 15 is connected to the liquid inlet end of the waste liquid assembly 5 to facilitate the discharge of waste liquid or waste gas from the liquid / gas outlet 15 into the waste liquid assembly 5.

[0051] There are multiple dye solution assemblies 4. In the attached drawings of this embodiment, there are two dye solution assemblies 4, which are respectively used to provide two different dye solutions (dye solution A and dye solution B). The dye solution assembly 4 includes a linear moving member 41 and a dye solution syringe 42. The linear moving member 41 is electrically connected to the control circuit 8. The linear moving member 41 can adopt a stepping push rod 43 motor, an electric cylinder, a cylinder, an oil cylinder or other mechanisms that can achieve linear motion. The dye solution syringe 42 has a push rod 43. The injection port of the dye solution syringe 42 is connected to the liquid / gas inlet 14 through a one-way valve. The moving end of the linear moving member 41 is connected to the push rod 43. Therefore, when the linear moving member 41 is started, it can drive the push rod 43 to squeeze the inner cavity of the dye solution syringe 42, so that the dye solution is discharged into the liquid / gas inlet 14 from the injection port. At the same time, by limiting the working duration or moving distance of the linear moving member 41, the amount of dye solution discharged into the carrier grid storage plate 1 can be controlled.

[0052] The rinsing assembly 6 includes a water storage bottle, an air pump 71 and a second solenoid valve 62. The air pump 71 and the second solenoid valve 62 are both electrically connected to the control circuit 8. The water storage bottle is provided with an air inlet pipe and a liquid outlet pipe. The end of the air inlet pipe is located above the liquid level of the liquid in the water storage bottle. The end of the liquid outlet pipe is close to the inner bottom surface of the liquid storage bottle 61. The air outlet end of the air pump 71 is connected to the air inlet pipe through the second solenoid valve 62. The top end of the liquid outlet pipe is connected to the liquid / gas inlet 14 through a one-way valve. Therefore, when it is necessary to clean the carrier grid 16 in the carrier grid storage plate 1, by starting the air pump 71 and the second solenoid valve 62, air is introduced into the water storage bottle, and under the pressure of air pressure, clear water is discharged into the carrier grid storage plate 1 through the liquid outlet pipe for cleaning.

[0053] The exhaust assembly includes the above-mentioned air pump 71, a filter 72 and a first solenoid valve 73. The first solenoid valve 73 is also electrically connected to the control circuit 8. The filter 72 is connected to the air inlet end of the air pump 71. The air outlet end of the air pump 71 is connected to the liquid / gas inlet 14 through the first solenoid valve 73 and a one-way valve. Therefore, when it is necessary to dry the inside of the carrier grid storage plate 1, by starting the air pump 71 and the first solenoid valve 73, after filtering impurities through the filter 72, impurity-free air is discharged into the inside of the carrier grid storage plate 1 for drying.

[0054] The waste liquid assembly 5 includes a waste liquid bottle 51 and an exhaust pipe 52. The waste liquid bottle 51 is connected to the liquid / gas outlet 15, and the exhaust pipe 52 is connected to the waste liquid bottle 51. Therefore, the dyeing waste liquid, cleaning waste liquid, and air in the carrier grid storage plate 1 can be discharged to the waste liquid bottle 51 through the liquid / gas outlet 15 for collection, and the air can be discharged outside the waste liquid bottle 51 through the exhaust pipe 52.

[0055] In summary, for the high-throughput carrier grid 16 dyeing instrument of the present utility model, by providing a number of interconnected storage slots 11 in the carrier grid storage plate 1, and simultaneously providing a liquid / gas inlet 14 and a liquid / gas outlet 15 that are both connected to the storage slots 11, several carrier grids 16 can be respectively placed in several storage slots 11 for storage. Then, through the sealing member provided on the surface of the carrier grid storage plate 1 to cover the openings of several storage slots 11, several interconnected storage slots 11 are in a relatively airtight space. Thus, by introducing dyeing liquid, cleaning water, or air into the carrier grid storage plate 1 through the liquid / gas inlet 14, operations such as dyeing, cleaning, or drying the carrier grid 16 can be achieved, and the generated waste water and waste gas can be discharged through the liquid / gas outlet 15. Compared with the prior art where the storage function and dyeing function of the carrier grid 16 are realized through different structures, the present utility model can unify and combine the storage and dyeing functions of the carrier grid 16 in the same structure, which has the effects of simple operation and cost savings.

[0056] Embodiment 2

[0057] A dyeing method for a high-throughput carrier grid 16 dyeing instrument, using the high-throughput carrier grid 16 dyeing instrument of Embodiment 1, includes the following steps:

[0058] 1) Storage of the carrier grid 16: Set the ultra-thin sections on the carrier grid 16, use tweezers to clamp the ring width position of the carrier grid 16, and place multiple carrier grids 16 in multiple storage slots 11 for storage respectively. Cover the surface of the carrier grid storage plate 1 with the sealing member to cover the openings of the storage slots 11, so that several interconnected storage slots 11 are in a relatively airtight space.

[0059] Before step 1) the storage of the carrier grid, the following steps are further included:

[0060] Preparation before dyeing: Start the air pump 71 and the second solenoid valve 62 to discharge the cleaning water in the water storage bottle into the carrier grid storage plate 1 to wash each storage slot 11, and the washed cleaning water is discharged to the waste liquid bottle 51 through the liquid / gas outlet 15; then start the air pump 71 and the first solenoid valve 73 to discharge air into the carrier grid storage plate 1 to dry each storage slot 11 in the carrier grid storage plate 1. Thus, the pre-cleaning and drying operation of the carrier grid storage plate 1 before dyeing is realized, improving the subsequent dyeing quality. It should be noted that this cleaning step before dyeing is a non-essential step and needs to be determined according to the cleanliness of the carrier grid storage plate 1 and the test accuracy.

[0061] 2) Staining of the specimen grid 16: Inject the staining solution into the specimen grid storage plate 1 through the liquid / gas inlet 14 so that each storage tank 11 is filled with the staining solution and the ultra-thin sections on the specimen grid 16 are stained.

[0062] During the process of injecting the staining solution into the specimen grid storage plate 1 through the liquid / gas inlet 14 for staining the specimen grid 16, the following method is adopted: Start the linear moving member 41 to discharge the staining solution A in one of the staining solution syringes 42 from the liquid / gas inlet 14 into the specimen grid storage plate 1 for primary staining. After the primary staining, inject clean water into the specimen grid storage plate 1 through the liquid / gas inlet 14 to clean and rinse each storage tank 11, and then start the other linear moving member 41 to discharge the staining solution B in the other staining solution syringe 42 from the liquid / gas inlet 14 into the specimen grid storage plate 1 for secondary staining.

[0063] 3) Cleaning of the specimen grid: The used staining solution is discharged from the liquid outlet. Then, inject clean water into the specimen grid storage plate 1 through the liquid / gas inlet 14 to clean each storage tank 11. It should be noted that during the staining process of the specimen grid 16 in step 2), between adjacent staining processes, step 3) cleaning of the specimen grid 16 is required to reduce the contamination of the staining process by the previous staining solution and improve the staining quality.

[0064] 4) Post-staining treatment: The used staining solution is discharged from the liquid / gas outlet 15. Then, inject clean water and air into the specimen grid storage plate 1 through the liquid / gas inlet 14 respectively to clean and dry each storage tank 11.

[0065] During the process of cleaning and drying the specimen grid storage plate 1 and its storage tanks 11, the following method is adopted: Start the air pump 71 and the second solenoid valve 62 to discharge the clean water in the water storage bottle into the specimen grid storage plate 1 to rinse each storage tank 11, and the rinsed clean water is discharged into the waste liquid bottle 51 from the liquid / gas outlet 15; at the same time, start the air pump 71 and the first solenoid valve 73 to discharge air into the specimen grid storage plate 1 to dry each storage tank 11 in the specimen grid storage plate 1.

[0066] In summary, the dyeing method of the present utility model mainly consists of four steps: preparation before dyeing, storage of the carrier net 16, dyeing of the carrier net 16, and post-dyeing treatment; before dyeing, the storage operation of the carrier net 16 can be achieved through multiple storage slots 11 of the carrier net storage plate 1. During the dyeing process, the dyeing solution can be introduced into the carrier net storage plate 1 through the liquid / gas inlet 14, so that the dyeing solution is introduced into several interconnected storage slots 11 to synchronously dye the carrier net 16 and its ultra-thin sections; at the same time, before and after dyeing, clean water and air can be introduced into the carrier net storage plate 1 through the liquid / gas inlet 14, so as to synchronously wash and dry multiple storage slots 11 and the carrier net 16; therefore, it has the beneficial effect of simple and efficient operation.

[0067] The implementation mode of the present utility model is not limited to this. According to the above content of the present utility model, using the common technical knowledge and conventional means in the art, without departing from the above basic technical idea of the present utility model, the present utility model can also be modified, replaced or combined in many other forms, all of which fall within the scope of the patent protection of the present utility model.

Claims

1. A high-throughput web staining instrument, characterized in that: It comprises a grid storage plate and a sealing component, wherein a plurality of interconnected storage slots are provided on the top surface of the grid storage plate, and a liquid inlet / gas port and a liquid / gas outlet are provided on the grid storage plate, both of which are interconnected with the storage slots, and the sealing component is arranged on the surface of the grid storage plate; the storage slots are used to store grids, and the sealing component covers the slots of a plurality of the storage slots to make the plurality of interconnected storage slots relatively closed, and the grid and its ultra-thin sections are stained by discharging staining liquid into the liquid inlet / gas port so that the plurality of the storage slots are filled with staining liquid, and after staining is completed, the staining liquid is discharged from the liquid / gas outlet, and cleaning and drying operations are simultaneously realized through the liquid inlet / gas port and the liquid / gas outlet.

2. The high-throughput web staining instrument according to claim 1, characterized in that: The sealing member comprises an upper splint and a lower splint, the upper splint is hinged to the lower splint via a hinge, a locking member is provided on the side of the upper splint away from the hinge, a receiving groove is provided on the lower splint, the mesh storage plate is arranged in the receiving groove, the liquid / gas inlet and the liquid / gas outlet of the mesh storage plate extend to the outside of the lower splint, after the upper splint is closed, the locking member is detachably connected to the lower splint, and the upper splint covers the notches of several storage grooves.

3. The high-throughput web staining instrument according to claim 2, characterized in that: The upper clamping plate is provided with a sinking groove corresponding to the accommodating groove, and the inner bottom surfaces of the accommodating groove and the sinking groove are both provided with sealing rubber pads. When the upper clamping plate is closed and locked by the locking member, the two sealing rubber pads clamp the grid storage plate.

4. The high-throughput web staining apparatus according to claim 2, characterized in that: A locking hook is arranged on the side of the lower clamping plate away from the hinge, and the locking member comprises a quick lock buckle, which is arranged on the side of the upper clamping plate away from the hinge. When the upper clamping plate is closed, the quick lock buckle is rotated to hook with the locking hook.

5. The high-throughput screen staining instrument according to claim 1, characterized in that: The plurality of storage slots on the mesh storage plate are distributed in an array, and a plurality of connecting slots are provided on the top surface of the mesh storage plate; in a row of the storage slots, a plurality of connecting slots are distributed at intervals, and the connecting slots are connected to adjacent storage slots, and U-shaped slots are provided between adjacent storage slots, and the connecting slots and the U-shaped slots are staggered in a row of the storage slots, so that a row of the storage slots are connected through the connecting slots and the U-shaped slots; connecting slots are provided between a plurality of rows of the storage slots, so that a plurality of the storage slots on the mesh storage plate are connected, and the liquid / gas inlet and the liquid / gas outlet are both provided on the bottom surface of the mesh storage plate.

6. The high-throughput screen staining instrument according to claim 1 or 2, characterized in that: It also includes a dye liquid component, a rinsing component, a ventilation component and a waste liquid component. The liquid outlet end of the dye liquid component, the liquid outlet end of the rinsing component and the air outlet end of the ventilation component are all connected to the liquid inlet / air port, and the liquid outlet / air port is connected to the liquid inlet end of the waste liquid component.

7. The high-throughput web staining instrument according to claim 6, characterized in that: The dye liquid components are multiple and are respectively used to provide two different dyes. The dye liquid components include a linear moving member and a dye liquid injector. The dye liquid injector has a push rod. The injection port of the dye liquid injector is connected to the liquid / gas inlet through a one-way valve. The moving end of the linear moving member is connected to the push rod. The rinsing assembly includes a water storage bottle, an air pump and a second solenoid valve, the water storage bottle is provided with an air inlet pipe and a liquid outlet pipe, the end of the air inlet pipe is located above the liquid level in the water storage bottle, the end of the liquid outlet pipe is close to the inner bottom surface of the liquid storage bottle, the air outlet end of the air pump is connected to the air inlet pipe through the second solenoid valve, and the top end of the liquid outlet pipe is connected to the liquid / air inlet through a one-way valve; The exhaust assembly includes the air pump, a filter and a first solenoid valve, the filter is connected to the air inlet end of the air pump, and the air outlet end of the air pump is connected to the liquid / gas inlet interface through the first solenoid valve and a one-way valve; The waste liquid component comprises a waste liquid bottle, and the waste liquid bottle is connected to the liquid / gas outlet.

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