Multi-row reaction device for improving polypeptide synthesis efficiency

Abstract

The utility model discloses a multi-row reaction device for improving polypeptide synthesis efficiency, which comprises a base, a rotating base is arranged above the base, two ends of the rotating base are respectively provided with an electric telescopic rod, the two electric telescopic rods respectively enable the end part of the rotating base to ascend or descend, a rotating disc is arranged in the rotating base, and the rotating disc is connected with the rotating base. A plurality of sleeves for placing reaction columns are arranged at the top of the rotating disc. A polypeptide reaction liquid in the reaction column is uniformly and omnidirectionally shaken, so that the reaction synthesis rate is finally accelerated, the reaction of multiple rows of polypeptides is realized, and the utilization rate of the device is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of polypeptide synthesis, and particularly relates to a multi-row reaction device for improving the efficiency of polypeptide synthesis. Background Art

[0002] Polypeptide is a bioactive substance related to various cell functions in organisms. Its molecular structure is between amino acids and proteins, and it is a compound formed by multiple amino acids combined by peptide bonds in a certain arrangement order. Polypeptide is a general term for bioactive substances related to various cell functions in organisms, and is often applied to fields such as functional analysis, antibody research, especially drug research and development.

[0003] Currently, in polypeptide synthesis devices, existing reaction devices often accommodate a single polypeptide reaction column for reaction. However, in reality, there are multiple polypeptide reaction columns that need to react, so multi-row reaction column reactions cannot be carried out. At the same time, the single shaking effect cannot accelerate the polypeptide synthesis rate. Summary of the Utility Model

[0004] The purpose of the utility model is to provide a multi-row reaction device for improving the efficiency of polypeptide synthesis. By setting the rotating disk in the rotating base to rotate horizontally and the electric telescopic rods to make the two ends of the rotating base shake reciprocally at different heights, the shaking of the reaction column in the horizontal and vertical directions is realized, thereby improving the reaction rate and solving the problems existing in the prior art.

[0005] To solve the above technical problems, the utility model adopts the following solutions:

[0006] A multi-row reaction device for improving the efficiency of polypeptide synthesis, including a base. A rotating base is provided above the base. Electric telescopic rods are respectively provided at both ends of the rotating base, and the two electric telescopic rods respectively raise or lower the ends of the rotating base. A rotating disk is provided inside the rotating base, and a plurality of sleeves for placing reaction columns are provided at the top of the rotating disk.

[0007] Further, a placement rack for carrying a motor is provided at the bottom of the rotating base, and the telescopic end of the motor sequentially penetrates through the through hole of the rotating base and the center of the rotating disk.

[0008] Further, a bearing is provided between the through hole and the output end of the motor.

[0009] Further, the rotating base is provided with a rotating groove, and the rotating disk is located in the rotating groove.

[0010] Further, an annular groove is provided on the side wall of the rotating groove, and a sliding block adapted to the annular groove is provided at the end of the rotating disk.

[0011] Further, a plurality of clamping assemblies are provided on the inner wall of the sleeve, a heating resistance wire is provided between two clamping assemblies, the clamping assembly includes a piston seat, a piston rod connected to a first spring is provided in the piston seat, a clamping plate is provided at an end of the piston rod away from the first spring, and a side surface of the clamping plate contacts the outer peripheral surface of the reaction column.

[0012] Further, a protrusion is provided on a side surface of the clamping plate that contacts the outer peripheral surface of the reaction column.

[0013] Further, a plurality of second springs are provided at the bottom of the sleeve, a buffer plate is provided at the top of the second springs, and the bottom of the reaction column is located on the surface of the buffer plate.

[0014] Further, a controller is provided on the surface of the base, and the controller is electrically connected to the electric telescopic rod and the motor respectively.

[0015] The beneficial effects of the present utility model are as follows:

[0016] In the present utility model, by setting the electric telescopic rod and the motor, the electric telescopic rod makes the two ends of the rotating base alternately rise and fall one high and one low to perform vertical shaking, and the output end of the motor makes the rotating disk perform horizontal rotation shaking, so as to realize the uniform and all-round shaking of the polypeptide reaction solution in the reaction column, finally accelerate the reaction synthesis rate, realize the reaction of multiple rows of polypeptides, and improve the utilization rate of the device.

[0017] The clamping assembly arranged in the sleeve, after the clamping plate receives a thrust, compresses the first spring, so that the piston rod contracts inside the piston seat, thereby firmly clamping the reaction column, and is suitable for clamping reaction columns of multiple specifications. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 is a schematic structural diagram of the present utility model;

[0019] Figure 2 is a top view structural diagram of the rotating disk of the present utility model;

[0020] Figure 3 is a top view structural diagram of the sleeve of the present utility model;

[0021] Figure 4 is a sectional view structural diagram of the sleeve of the present utility model;

[0022] Figure 5 is a partial enlarged structural diagram of the clamping assembly of the present utility model.

[0023] Reference numerals: 1 - base, 10 - controller, 2 - electric telescopic rod, 3 - rotating base, 30 - rotating groove, 31 - through hole, 32 - bearing, 33 - placement rack, 34 - motor, 35 - annular groove, 4 - rotating disc, 40 - sleeve, 41 - clamping assembly, 410 - piston seat, 411 - piston rod, 412 - first spring, 413 - clamping plate, 4130 - protrusion, 42 - heating resistance wire, 43 - second spring, 44 - buffer plate, 46 - sliding block, 5 - reaction column. Detailed implementation manners

[0024] The following will further describe the present utility model in detail in conjunction with embodiments and the accompanying drawings, but the implementation manners of the present utility model are not limited thereto.

[0025] In the description of the present utility model, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "longitudinal", "lateral", "horizontal", "inner", "outer", "front", "rear", "top", "bottom", etc. is based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship in which the product of the present utility model is usually placed during use. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present utility model.

[0026] In the description of the present utility model, it should also be noted that unless otherwise clearly specified and limited, the terms "set", "provided with", "installed", "connected", "connected to" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific situations.

[0027] Embodiment 1

[0028] Embodiment 1 of the present utility model is a multi - row reaction device for improving the efficiency of polypeptide synthesis, including a base 1. Above the base 1, there is a rotating base 3. At both ends of the rotating base 3, there are respectively electric telescopic rods 2. The two electric telescopic rods 2 respectively raise or lower the ends of the rotating base 3. Inside the rotating base 3, there is a rotating disc 4. On the top of the rotating disc 4, there are multiple sleeves 40 for placing reaction columns 5.

[0029] Refer to Figure 1 And Figure 2, the multi-row reaction device of the present utility model mainly realizes the height adjustment of the rotating base 3 connected to the base 1 by setting two electric telescopic rods 2 to raise or lower respectively. That is, the two ends of the rotating base 3 are not on the same horizontal plane during the shaking process, and the two ends alternate up and down with one end higher and the other lower, so that the rotating base 3 drives the rotating disk 4 and the reaction column 5 located above it to perform height adjustment, thereby realizing the shaking in the vertical direction and finally accelerating the polypeptide reaction rate in the reaction column 5. At the same time, the rotating disk 4 rotates horizontally above the rotating base 3, so that the polypeptides in the reaction column 5 are mixed evenly. Thus, the shaking in the vertical direction is combined with the rotating shaking in the horizontal direction, thereby accelerating the reaction rate of the polypeptides, realizing the reaction of multiple rows of polypeptides, and improving the working efficiency.

[0030] In some preferred embodiments, a placement rack 33 for carrying the motor 34 is provided at the bottom of the rotating base 3, and the output end of the motor 34 sequentially passes through the through hole 31 of the rotating base 3 and the center of the rotating disk 4. A bearing 32 is provided between the through hole 31 and the telescopic end of the motor 34. The rotating base 3 is provided with a rotating groove 30, and the rotating disk 4 is located in the rotating groove 30.

[0031] Specifically, the rotating disk 4 is mainly located inside the rotating groove 30 of the rotating base 3, and at the same time, under the action of the motor 34, the rotating disk 4 rotates horizontally. And the bearing 32 provided at the through hole 31 where the output end of the motor 34 passes through the rotating base 3 provides space for the rotation of the output end of the motor 34 at this position of the through hole 31. The placement rack 33 provided on the rotating base 3 enables the motor 34 to change with the height change of the rotating base 3 during the lifting process of the electric telescopic rod 2, and the output end of the motor 34 is always perpendicular to the rotating disk 4, thereby ensuring that the output end of the motor 34 rotates the rotating disk 4 in the rotating groove 30.

[0032] It should be noted that since the electric telescopic rod 2 is connected to the rotating base 3, the electric telescopic rod 2 enables the rotating base 3 to perform vertical lifting activities and does not perform any activities in the horizontal direction.

[0033] In some preferred embodiments, an annular groove 35 is provided on the side wall of the rotating groove 30, and a sliding block 46 adapted to the annular groove 35 is provided at the end of the rotating disk 4.

[0034] Specifically, referring to Figure 1 , the sliding block 46 is located inside the annular groove 35, and the number of the sliding blocks 46 is at least two and they are symmetric with each other, so that during the rotation of the rotating disk 4, the sliding blocks 46 rotate inside the annular groove 35, enhancing the connection stability between the rotating disk 4 and the rotating base 3.

[0035] Embodiment 2

[0036] This Example 2 is implemented on the basis of Example 1. A plurality of clamping components 41 are provided on the inner wall of the sleeve 40. A heating resistance wire 42 is provided between two clamping components 41. The clamping component 41 includes a piston seat 410. A piston rod 411 connected to a first spring 412 is provided in the piston seat 410. A clamping plate 413 is provided at the end of the piston rod 411 away from the first spring 412. The side surface of the clamping plate 413 contacts the outer peripheral surface of the reaction column 5. A protrusion 4130 is provided on the side surface of the clamping plate 413 that contacts the outer peripheral surface of the reaction column 5.

[0037] Referring to Figures 3 to 5 , the sleeve 40 is fixedly connected to the surface of the rotating disk 4. The clamping components 41 provided in the sleeve 40 can accommodate the placement of reaction columns 5 of multiple specifications, thereby improving the reuse rate of the device. Specifically, the clamping is mainly achieved through the piston seat 410, the piston rod 411, and the first spring 412. First, the bottom of the reaction column 5 is placed in the sleeve 40. After the clamping plate 413 receives the thrust from the reaction column 5, the first spring 412 is compressed, causing the piston rod 411 to contract inside the piston seat 410, thereby firmly clamping the reaction column 5.

[0038] Moreover, in order to enhance the clamping stability of the clamping plate 413 on the reaction column 5, a plurality of protrusions 4130 are provided to increase the friction between the clamping plate 413 and the outer peripheral surface of the reaction column 5 and prevent detachment during shaking.

[0039] Clamping components 41 in the same vertical direction are also provided inside the sleeve 40 to ensure that the reaction column 5 is clamped in both the horizontal and vertical planes, thereby enhancing the clamping stability. The heating resistance wire 42 is specifically set according to the actual temperature requirements of the polypeptide reaction solution, so that under certain temperature conditions, the reaction rate of the polypeptide reaction solution in the reaction column 5 is accelerated and the reaction time is shortened.

[0040] In some preferred embodiments, a plurality of second springs 43 are provided at the bottom of the sleeve 40. A buffer plate 44 is provided at the top of the second spring 43. The bottom of the reaction column 5 is located on the surface of the buffer plate 44. The second spring 43 and the buffer plate 44 are mainly provided to provide a buffering effect on the reaction column 5 during shaking, to avoid the bottom end of the reaction column 5 directly contacting the bottom of the sleeve 40, to prevent damage during shaking, and to avoid leakage of the polypeptide liquid in the reaction column 5, which may affect the reaction.

[0041] In some preferred embodiments, a controller 10 is provided on the surface of the base 1, and the controller 10 is electrically connected to the electric telescopic rod 2 and the motor 34 respectively. Under the action of an external power source, the controller 10 realizes the reciprocating lifting of the electric telescopic rod 2 and the rotational operation of the output end of the motor 34. At the same time, the controller 10 can also start the heating resistance wire 42 to perform heating work. The control function of the controller 10 is prior art and will not be elaborated here.

[0042] The working principle of the present utility model is as follows: During use, the reaction column 5 containing the polypeptide reaction solution is placed inside the sleeve 40. After the clamping plate 413 inside the sleeve 40 receives a thrust, the first spring 412 is compressed, causing the piston rod 411 to contract inside the piston seat 410, thereby firmly clamping the reaction column 5 and enabling the clamping of reaction columns 5 of multiple specifications; after placing multiple reaction columns 5, the controller 10 is connected to an external power source, the electric telescopic rod 2 and the motor 34 are started to work, and the heating resistance wire 42 is turned on for heating. The electric telescopic rod 2 causes the two ends of the rotating base 3 to alternately lift one high and one low, thereby driving the rotating disk 4 and the reaction column 5 located above it to lift in height. The output end of the motor 34 causes the rotating disk 4 to rotate and shake in the horizontal direction, realizing the uniform and all-round shaking of the polypeptide reaction solution in the reaction column 5, ultimately accelerating the reaction synthesis rate, realizing the reaction of multiple rows of polypeptides, and improving the utilization rate of the device.

[0043] The above is only a preferred embodiment of the present utility model and does not impose any form of limitation on the present utility model. Based on the technical essence of the present utility model, any simple modification, equivalent replacement, and improvement made to the above embodiments within the spirit and principle of the present utility model still fall within the protection scope of the technical solution of the present utility model.

Claims

1. A multi-row reaction device for improving the efficiency of polypeptide synthesis, characterized in that: The invention comprises a base (1), a rotating base (3) is arranged above the base (1), electric telescopic rods (2) are respectively arranged at both ends of the rotating base (3), and the two electric telescopic rods (2) respectively raise or lower the ends of the rotating base (3), a rotating disk (4) is arranged inside the rotating base (3), and a plurality of sleeves (40) for placing reaction columns (5) are arranged on the top of the rotating disk (4).

2. A multi-row reaction device for improving polypeptide synthesis efficiency according to claim 1, characterized in that: A placement frame (33) for carrying a motor (34) is provided at the bottom of the rotating base (3), and the telescopic end of the motor (34) passes through the through hole (31) of the rotating base (3) and the center of the rotating disk (4) in sequence.

3. A multi-row reaction device for improving polypeptide synthesis efficiency according to claim 2, characterized in that: A bearing (32) is provided between the through hole (31) and the output end of the motor (34).

4. A multi-row reaction device for improving polypeptide synthesis efficiency according to claim 2, characterized in that: The rotating base (3) is provided with a rotating groove (30), and the rotating disk (4) is located in the rotating groove (30).

5. A multi-row reaction device for improving polypeptide synthesis efficiency according to claim 4, characterized in that: The side wall of the rotating groove (30) is provided with an annular groove (35), and the end of the rotating disk (4) is provided with a sliding block (46) matched with the annular groove (35).

6. A multi-row reaction device for improving polypeptide synthesis efficiency according to claim 4, characterized in that: The inner wall of the sleeve (40) is provided with a plurality of clamping assemblies (41), a heating resistance wire (42) is provided between two clamping assemblies (41), the clamping assembly (41) comprises a piston seat (410), a piston rod (411) connected to a first spring (412) is provided inside the piston seat (410), a clamping plate (413) is provided at the end of the piston rod (411) away from the first spring (412), and the side surface of the clamping plate (413) is in contact with the outer peripheral surface of the reaction column (5).

7. A multi-row reaction device for improving polypeptide synthesis efficiency according to claim 6, characterized in that: A protrusion (4130) is provided on the side of the clamping plate (413) that contacts the outer peripheral surface of the reaction column (5).

8. A multi-row reaction device for improving polypeptide synthesis efficiency according to claim 6, characterized in that: A plurality of second springs (43) are provided at the bottom of the sleeve (40), a buffer plate (44) is provided at the top of the second spring (43), and the bottom of the reaction column (5) is located on the surface of the buffer plate (44).

9. A multi-row reaction device for improving polypeptide synthesis efficiency according to claim 6, characterized in that: A controller (10) is provided on the surface of the base (1), and the controller (10) is electrically connected to the electric telescopic rod (2) and the motor (34) respectively.

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