Symbiotic bacterium mediated plant root exudates collecting device

Abstract

The utility model discloses a symbiotic bacterium mediated plant root exudate collecting device, which belongs to the technical field of sampling devices and comprises a collecting device provided with a flowerpot component. A soil sampling assembly is mounted in the flowerpot assembly; a bracket assembly is erected at the bottom of the flowerpot assembly; the flowerpot assembly comprises a flowerpot, a first communicating vessel, a second communicating vessel, a first gauze layer, a partition plate and a communicating hole. Communicating holes are formed in the lower ends of the flowerpots, the two ends of the first communicating vessel are communicated with the flowerpot on the left side and the flowerpot in the middle respectively, the two ends of the second communicating vessel are communicated with the flowerpot in the middle and the flowerpot on the right side respectively, a first gauze layer is fixedly connected into the first communicating vessel, and a partition plate is fixedly connected into the second communicating vessel. According to the device, various data of a plant to be researched in a symbiotic bacterium environment and a non-symbiotic bacterium environment can be measured through a self-contrast experiment, and whether a hypha bridge can influence plant root exudates or not is judged according to a research result.

Description

Technical Field

[0001] The utility model relates to the technical field of sampling devices, in particular to a device for collecting plant root exudates mediated by symbiotic bacteria. Background Art

[0002] Pot experiments are a method of planting, observing, measuring and studying plants in a potted environment. It is a controlled experimental condition that can simulate important factors such as soil, climate, moisture and organisms in the natural environment in order to conduct scientific research on plant growth and development. Plant root exudates are the medium in the processes of material exchange, energy flow and information transmission, and are the key to alleviating certain stresses. Plant root exudates are closely related to plant growth. To explore whether the root exudates mediated by symbiotic bacteria and non-symbiotic bacteria in pot experiments change, and to explore the change mechanism, it is very important to collect the root exudates and conduct physical and chemical property tests; therefore, a device for collecting root exudates is needed to collect and study the root exudates.

[0003] Chinese patent CN211576592U discloses a sampling device for collecting root exudates. Its structure achieves the effect of distinguishing different root and stem parts by setting a connecting block, a connecting hole and a through hole, and achieves the purpose of cutting the root and stem through the connecting block.

[0004] However, its structure lacks a self-comparison experiment and cannot measure diverse data of the plant to be studied in the connected and non-connected states of the roots through one experiment; secondly, its structure obtains different detection results by detecting the root and stem at different positions, but the results cannot directly judge the influence of the plant root and stem on other factors such as soil and culture solution.

[0005] Based on this, the utility model designs a device for collecting plant root exudates mediated by symbiotic bacteria to solve the above problems. Summary of the Utility Model

[0006] In view of the above-mentioned drawbacks of the prior art, the utility model provides a device for collecting plant root exudates mediated by symbiotic bacteria.

[0007] To achieve the above object, the utility model is realized through the following technical solutions:

[0008] A device for collecting plant root exudates mediated by symbiotic bacteria includes a collection device, and a flower pot assembly for culturing symbiotic bacteria is installed on the collection device;

[0009] A soil sampling assembly is installed inside the flower pot assembly;

[0010] A support assembly for supporting the overall mechanism is erected at the bottom of the flower pot assembly;

[0011] The flowerpot assembly includes flowerpots, a first communicating vessel, a second communicating vessel, a first gauze layer, a partition board, and communicating holes; there are three flowerpots, and communicating holes communicating with the bottom are opened at the lower ends of the flowerpots. The two ends of the first communicating vessel are respectively communicated with the left flowerpot and the middle flowerpot, and the two ends of the second communicating vessel are respectively communicated with the middle flowerpot and the right flowerpot. A first gauze layer is fixedly connected inside the first communicating vessel, and a partition board is fixedly connected inside the second communicating vessel.

[0012] Furthermore, the soil sampling assembly includes a sampling outer cylinder, a sampling inner cylinder, a soil cutting inclined surface, an L-shaped ring, a fixing block, and a handle; the sampling outer cylinder and the sampling inner cylinder are placed inside the flowerpot; the bottom of the sampling outer cylinder and the bottom of the sampling inner cylinder are rotationally connected through a rotating shaft. Handles are fixedly connected to the tops of the sampling outer cylinder and the sampling inner cylinder, and a soil cutting inclined surface is arranged on the side wall of the sampling inner cylinder.

[0013] Furthermore, the bottom of the sampling outer cylinder is close to the inner bottom of the flowerpot.

[0014] Furthermore, an L-shaped ring is fixedly connected to the top of the sampling inner cylinder, a fixing block is fixedly connected to the top of the sampling outer cylinder, and the fixing block is slidably connected to the inner wall of the L-shaped ring.

[0015] Furthermore, the flowerpots, the first communicating vessel, and the second communicating vessel are made of PVC material; the sampling outer cylinder and the sampling inner cylinder are made of corrosion-resistant materials.

[0016] Furthermore, the bracket assembly includes an annular plate, a strip-shaped plate, and a second gauze layer; there are multiple annular plates and strip-shaped plates. The annular plates and the strip-shaped plates are fixedly connected, and the annular plates and the strip-shaped plates are arranged in a grid pattern. A second gauze layer is fixedly connected to the top of the bottom annular plate.

[0017] Furthermore, the bottom of the top annular plate is located at the top of the collection device; the bottom of the flowerpot is located at the top of the bottom annular plate.

[0018] Furthermore, the number of the communicating holes is 8 - 10, and the diameter is 0.5 - 1 cm; the diameters of the first communicating vessel and the second communicating vessel are both 2.5 - 5 cm, and the lengths are both 8 - 10 cm.

[0019] The utility model has the following technical effects:

[0020] 1. In this utility model, the plants to be studied are respectively planted in three flower pots of the flower pot assembly. The first gauze layer in the first communicating vessel can support the plant rhizomes to pass through, but cannot support the soil to pass through. The establishment of the first gauze layer enables the flower pots on the left and in the middle to form a symbiotic bacteria environment, and at the same time prevents the soil from flowing out; the partition in the second communicating vessel neither supports the rhizomes to pass through nor supports the soil to pass through. The establishment of the partition makes the flower pot on the right become a non-symbiotic bacteria environment; through its own comparative experiment, this structure can measure a variety of data of the plants to be studied in the symbiotic bacteria environment and the non-symbiotic bacteria environment through one experiment, and judge whether the hyphal bridge will affect the plant root exudates through the research results.

[0021] 2. When planting the plants to be studied in this utility model, the sampling outer cylinder and the sampling inner cylinder of the soil sampling assembly are placed in the soil, and the upper surface height of the soil is slightly lower than the bottom of the handle. When it is necessary to detect the soil, hold the two handles with both hands respectively, rotate the two handles in a mirror image of each other, so that the sampling outer cylinder and the sampling inner cylinder are combined into a cylinder, and then pull out the sampling outer cylinder and the sampling inner cylinder. Take a small amount of the pulled-out soil for detection, and then put the sampling outer cylinder and the sampling inner cylinder back to their original positions, and rotate the handle again to reset the sampling outer cylinder and the sampling inner cylinder. The cutting slope can reduce the force required to rotate the handle, making the overall structure convenient for the staff to use. The L-shaped ring, the fixing block and the rotating shaft are used to provide limits; when detecting, this structure can detect the plant rhizomes and the soil at different heights at the same time, and can detect various elements such as water, culture solution and root exudates in the soil at different heights. At the same time, after the detection is completed, this structure can reset the soil, which can reduce the impact of soil detection on plant growth.

[0022] 3. The annular plate of the support assembly of this utility model is placed on the top of the collection device, and the flower pots of the flower pot assembly are placed on the top of the annular plate at the bottom. The communication holes opened in the flower pots can pass through the soil, culture solution and root exudates, but the second gauze layer can only pass through the culture solution and root exudates. Therefore, while preventing the soil from flowing away, the second gauze layer can make the culture solution and root exudates flow into the interior of the collection device; this structure is simple to install and convenient to use, and is easy to assemble and disassemble. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the following described drawings are only some embodiments of the present utility model. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0024] Figure 1 For the three-dimensional view of the present utility model Figure 1 ;

[0025] Figure 2 It is the front view of the present utility model;

[0026] Figure 3 It is the right view of the present utility model;

[0027] Figure 4 It is a sectional view along the A-A direction of Figure 3 ;

[0028] Figure 5 It is the soil sampling assembly of the utility model;

[0029] Figure 6 It is the bracket assembly of the utility model.

[0030] The reference numerals in the figure respectively represent:

[0031] 1. Collection device; 2. Flower pot assembly; 21. Flower pot; 22. First communicating vessel; 23. Second communicating vessel; 24. First gauze layer; 25. Partition board; 26. Communication hole; 3. Soil sampling assembly; 31. Sampling outer cylinder; 32. Sampling inner cylinder; 33. Soil cutting inclined surface; 34. L-shaped ring; 35. Fixed block; 36. Handle; 4. Bracket assembly; 41. Annular plate; 42. Strip-shaped plate; 43. Second gauze layer. Specific embodiments

[0032] To make the objectives, technical solutions and advantages of the embodiments of the present utility model clearer, the technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model. Apparently, the described embodiments are some but not all of the embodiments of the present utility model. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts shall fall within the protection scope of the present utility model.

[0033] The present utility model will be further described below with reference to the embodiments.

[0034] The "left", "right", "front", "rear", "upper" and "lower" mentioned in the following description are oriented in the Figure 2 viewing direction.

[0035] Please refer to the attached Figures 1-6 of the specification. A plant root exudate collection device mediated by symbiotic bacteria includes a collection device 1;

[0036] The collection device 1 is installed with a flower pot assembly 2 for culturing symbiotic bacteria;

[0037] The flowerpot assembly 2 includes a flowerpot 21, a first connector 22, a second connector 23, a first gauze layer 24, a partition 25, and a communication hole 26; there are three flowerpots 21, and a communication hole 26 communicating with the bottom is opened at the lower end of the flowerpot 21. The two ends of the first connector 22 are respectively communicated with the left flowerpot 21 and the middle flowerpot 21, and the two ends of the second connector 23 are respectively communicated with the middle flowerpot 21 and the right flowerpot 21. A first gauze layer 24 is fixedly connected inside the first connector 22, and a partition 25 is fixedly connected inside the second connector 23;

[0038] Preferably, the flowerpot 21, the first connector 22, and the second connector 23 are made of PVC material; this material has a low cost and is easy to manufacture;

[0039] Preferably, the number of the communication holes 26 is 8 - 10, the diameter is 0.5 - 1 cm, the diameters of the first connector 22 and the second connector 23 are 2.5 - 5 cm, and the length is 8 - 10 cm;

[0040] During use, the plants to be studied are respectively planted in the three flowerpots 21 of the flowerpot assembly 2. The first gauze layer 24 in the first connector 22 can support the plant rhizomes to pass through, but cannot support the soil to pass through. The establishment of the first gauze layer 24 enables the left flowerpot 21 and the middle flowerpot 21 to form a symbiotic bacteria environment, and at the same time prevents the soil from flowing out; the partition 25 in the second connector 23 neither supports the rhizomes to pass through nor supports the soil to pass through. The establishment of the partition 25 makes the right flowerpot 21 a non - symbiotic bacteria environment; through its own comparative experiment, this structure can measure a variety of data of the plants to be studied in the symbiotic bacteria environment and the non - symbiotic bacteria environment through one experiment, and judge whether the hyphal bridge will affect the plant root exudates through the research results;

[0041] A soil sampling component 3 is installed inside the flowerpot assembly 2;

[0042] The soil sampling component 3 includes a sampling outer cylinder 31, a sampling inner cylinder 32, a soil - cutting inclined surface 33, an L - shaped ring 34, a fixing block 35, and a handle 36; the sampling outer cylinder 31 and the sampling inner cylinder 32 are placed inside the flowerpot 21; the bottom of the sampling outer cylinder 31 and the bottom of the sampling inner cylinder 32 are rotationally connected through a rotating shaft. Handles 36 are fixedly connected to the tops of both the sampling outer cylinder 31 and the sampling inner cylinder 32. The handles 36 are located directly above the flowerpot 21. A soil - cutting inclined surface 33 is provided on the side wall of the sampling inner cylinder 32. An L - shaped ring 34 is fixedly connected to the top of the sampling inner cylinder 32, and a fixing block 35 is fixedly connected to the top of the sampling outer cylinder 31. The fixing block 35 is slidably connected to the inner wall of the L - shaped ring 34; the bottom of the sampling outer cylinder 31 is close to the inner bottom of the flowerpot 21;

[0043] Preferably, the sampling outer cylinder 31 and the sampling inner cylinder 32 are made of corrosion-resistant materials, which can extend the service life of the structure and prevent the culture environment from being polluted after the structure is corroded.

[0044] When planting the plants to be studied, place the sampling outer cylinder 31 and the sampling inner cylinder 32 of the soil sampling assembly 3 in the soil, with the upper surface of the soil slightly lower than the bottom of the handle 36. When it is necessary to detect the soil, hold the two handles 36 with both hands and rotate the two handles 36 mirror-image to each other, so that the sampling outer cylinder 31 and the sampling inner cylinder 32 are combined into a cylinder, then pull out the sampling outer cylinder 31 and the sampling inner cylinder 32, take a small amount of the pulled-out soil for detection, and then put the sampling outer cylinder 31 and the sampling inner cylinder 32 back to their original positions, and rotate the handle 36 again to reset the sampling outer cylinder 31 and the sampling inner cylinder 32. The cutting slope 33 can reduce the force required to rotate the handle 36, making the overall structure convenient for the staff to use. The L-shaped ring 34, the fixing block 35 and the rotating shaft are used for providing limits. When detecting, this structure can detect the plant roots and the soil at different heights at the same time, and can detect various elements such as water, culture solution and root exudates in the soil at different heights. At the same time, after the detection is completed, this structure can reset the soil, which can reduce the impact of soil detection on plant growth.

[0045] A support assembly 4 for supporting the overall mechanism is provided at the bottom of the flowerpot assembly 2.

[0046] The support assembly 4 includes an annular plate 41, a strip plate 42 and a second gauze layer 43. There are multiple annular plates 41 and strip plates 42. The annular plate 41 is fixedly connected to the strip plate 42, and the annular plate 41 and the strip plate 42 are arranged in a grid pattern. The bottom of the top annular plate 41 is located at the top of the collection device 1, and the top of the bottom annular plate 41 is fixedly connected with the second gauze layer 43. The bottom of the flowerpot 21 is located at the top of the bottom annular plate 41.

[0047] During use, place the annular plate 41 of the support assembly 4 on the top of the collection device 1, and place the flowerpot 21 of the flowerpot assembly 2 on the top of the bottom annular plate 41. The communication hole 26 opened in the flowerpot 21 can pass soil, culture solution and root exudates, but the second gauze layer 43 can only pass the culture solution and root exudates. Therefore, while preventing soil loss, the second gauze layer 43 can make the culture solution and root exudates flow into the interior of the collection device 1. This structure is simple to install and convenient to use, and is easy to assemble and disassemble.

[0048] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A symbiotic bacteria-mediated plant root exudate collection device, comprising a collection device (1), characterized in that: The collecting device (1) is equipped with a flowerpot assembly (2) for cultivating symbiotic bacteria; A soil sampling assembly (3) is installed inside the flower pot assembly (2); A bracket assembly (4) for supporting the entire structure is provided at the bottom of the flower pot assembly (2); The flower pot assembly (2) comprises a flower pot (21), a first communicating vessel (22), a second communicating vessel (23), a first gauze layer (24), a partition (25) and a connecting hole (26); three flower pots (21) are provided, a connecting hole (26) connected to the bottom is provided at the lower end of the flower pot (21), two ends of the first communicating vessel (22) are respectively connected to the flower pot (21) on the left and the flower pot (21) in the middle, two ends of the second communicating vessel (23) are respectively connected to the flower pot (21) in the middle and the flower pot (21) on the right, the first communicating vessel (22) is fixedly connected to the first gauze layer (24) inside, and the second communicating vessel (23) is fixedly connected to the partition (25) inside.

2. The symbiotic bacteria-mediated plant root exudate collection device according to claim 1, characterized in that: The soil sampling assembly (3) comprises a sampling outer cylinder (31), a sampling inner cylinder (32), a soil cutting slope (33), an L-shaped ring (34), a fixing block (35) and a handle (36); the sampling outer cylinder (31) and the sampling inner cylinder (32) are placed inside a flower pot (21); the bottom of the sampling outer cylinder (31) and the bottom of the sampling inner cylinder (32) are rotatably connected via a rotating shaft, the tops of the sampling outer cylinder (31) and the sampling inner cylinder (32) are fixedly connected with a handle (36), and the side wall of the sampling inner cylinder (32) is provided with a soil cutting slope (33).

3. The symbiotic bacteria-mediated plant root exudate collection device according to claim 2, characterized in that: The bottom of the sampling outer cylinder (31) is close to the inner bottom of the flower pot (21).

4. The symbiotic bacteria-mediated plant root exudate collection device according to claim 3, characterized in that: An L-shaped ring (34) is fixedly connected to the top of the sampling inner cylinder (32), and a fixing block (35) is fixedly connected to the top of the sampling outer cylinder (31). The fixing block (35) is slidably connected to the inner wall of the L-shaped ring (34).

5. The symbiotic bacteria-mediated plant root exudate collection device according to claim 4, characterized in that: The flower pot (21), the first communicating vessel (22) and the second communicating vessel (23) are made of PVC material.

6. The symbiotic bacteria-mediated plant root exudate collection device according to claim 5, characterized in that: The support assembly (4) comprises an annular plate (41), a strip plate (42) and a second gauze layer (43); there are a plurality of annular plates (41) and strip plates (42); the annular plates (41) are fixedly connected to the strip plates (42), and the annular plates (41) and the strip plates (42) are arranged in a grid, and the top of the bottom annular plate (41) is fixedly connected to the second gauze layer (43).

7. The symbiotic bacteria-mediated plant root exudate collection device according to claim 6, characterized in that: The bottom of the top annular plate (41) is located on the top of the collecting device (1); the bottom of the flower pot (21) is located on the top of the bottom annular plate (41).

8. The symbiotic bacteria-mediated plant root exudate collection device according to claim 7, characterized in that: The number of the communicating holes (26) is 8 to 10, and the diameter is 0.5 to 1 (cm); the diameters of the first communicating vessel (22) and the second communicating vessel (23) are both 2.5 to 5 (cm), and the lengths are both 8 to 10 (cm).

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