CTC enrichment dyeing auxiliary equipment

Abstract

The utility model relates to the technical field of biological medicine, in particular to CTC enrichment dyeing auxiliary equipment, which comprises a heating tank, a micro-fluidic device, a micro-fluidic control device, a micro-fluidic control device, a control device and a control device, and is characterized in that the heating tank is positioned in a mounting frame and is used for providing a constant-temperature environment for reagents in the micro-fluidic device; the liquid suction assembly is used for sucking a reagent from the test tube and adding the reagent into the microfluidic device in the heating tank; the moving assembly is arranged on the mounting frame and used for driving the liquid suction assembly to move in the horizontal direction and the vertical direction; the liquid suction assembly comprises a limiting frame and a plurality of liquid suction devices installed in the limiting frame, a movable plate is arranged at the lower end of the limiting frame, the movable plate is connected with the limiting frame through a reset spring, and the lower ends of the liquid suction devices penetrate through the limiting frame and the movable plate and are connected with liquid suction heads in a sleeving mode; two limiting blocks are fixedly connected to the opposite positions of the inner side face of the mounting frame. The utility model solves the problem of low efficiency of the existing CTC enrichment dyeing experiment.

Description

Technical Field

[0001] The utility model relates to the technical field of biomedicine, in particular to an auxiliary device for CTC enrichment and staining. Background Technique

[0002] The pharmaceutical industry and the biomedical engineering industry are the two major pillars of the modern pharmaceutical industry. The biomedical industry is jointly composed of the biotechnology industry and the pharmaceutical industry. Biomedical engineering comprehensively applies the principles and methods of life science and engineering science. From an engineering perspective, it understands the structure, function, and other life phenomena of the human body at multiple levels, including molecules, cells, tissues, organs, and even the entire human body system, and studies the general term for artificial materials, products, devices, and system technologies used for disease prevention, treatment, human function assistance, and health care.

[0003] In recent years, some emerging tumor diagnostic and detection technologies have emerged in the field of biomedical engineering, such as the detection methods of circulating tumor DNA and circulating tumor cells (CTCs) called liquid biopsy. For the detection method of circulating tumor cells, the current mainstream operation method is to enrich first and then detect. Among them, during the detection process, steps such as blotting, enrichment, and staining need to be performed on the collected body fluid, and reagents need to be added multiple times for each step, and there are many types of reagents. Currently, when the existing reagents on the market are taken and placed, they are all manually taken and placed, with low efficiency. At the same time, there is a lack of a reagent heat preservation device during the experiment, resulting in low reaction activity of the reagents. Both of these greatly reduce the reagent identification efficiency. Content of the Utility Model

[0004] In order to solve the above problems of the prior art, the utility model provides an auxiliary device for CTC enrichment and staining, which solves the problem of low efficiency in the current CTC enrichment and staining experiment.

[0005] To achieve the above object, the utility model provides the following technical solution: A CTC enrichment staining auxiliary device, comprising: a heating tank, which is located in a mounting frame and is used to provide a constant temperature environment for the reagents in the microfluidic device; a liquid suction assembly, which is used to suck the reagents from a test tube and add them into the microfluidic device in the heating tank; a moving assembly, which is arranged on the mounting frame and is used to drive the liquid suction assembly to move in the horizontal and vertical directions; the liquid suction assembly includes a limiting frame and several liquid suction devices installed inside the limiting frame, the lower end of the limiting frame is provided with a movable plate, the movable plate is connected to the movable plate through a return spring, the lower end of the liquid suction device passes through the limiting frame and the movable plate and is sleeved with a liquid suction head, two limiting blocks are fixedly connected to the opposite positions of the inner side surface of the mounting frame, the movable plate is located below the limiting blocks, the moving assembly includes a second lead screw and a slide rail arranged in parallel at the upper end of the mounting frame, a cross beam is slidably connected to the upper ends of the second lead screw and the slide rail, a first lead screw is fixedly connected to the middle position of the cross beam, the first lead screw is arranged vertically, and the limiting frame is slidably connected to the first lead screw.

[0006] With the above structural design, the moving assembly drives the liquid suction assembly to move in the horizontal and vertical directions, so that the liquid suction assembly can automatically extract and transfer the reagents, improving the efficiency of reagent picking and placing, and solving the problems of cumbersome and laborious manual operation and low efficiency. The cross beam is driven to move by the second lead screw, and then the liquid suction assembly is driven to move horizontally. The liquid suction assembly is driven to move vertically by the second lead screw.

[0007] Preferably, the liquid suction device includes a sleeve end and a connection end fixedly connected from bottom to top, the connection end passes through the limiting frame and is fixedly connected with a fixing bolt, the bottom end of the sleeve end is matched with the liquid suction head, a vacuum pipeline is arranged along the length direction of the liquid suction device, one end of the pipeline opens at the sleeve end, the other end of the pipeline opens at the fixing bolt and is connected with a vacuum pump through a communicating pipe, a compression spring is sleeved on the connection end, and the two ends of the compression spring respectively abut against the sleeve end and the limiting frame.

[0008] With the above structural design, as the slider of the first lead screw moves downward, the sleeve end gradually approaches the liquid suction head bracket below until the sleeve end is tightly attached to the liquid suction head. At this time, the compression spring plays a buffering role. After the liquid suction head is clamped at the lower end of the liquid suction device, the slider of the first lead screw moves upward, and the liquid suction head moves upward with the liquid suction device, realizing the automatic lapping operation of the liquid suction device and the liquid suction head. The motor drives the liquid suction assembly to move to the upper part of the reagent kit along the second lead screw. The slider of the first lead screw drives the liquid suction assembly to move downward into the reagent kit. The negative pressure assembly is turned on to suck the reagent. After the reagent is sucked, the first lead screw drives the liquid suction assembly to move upward to reset, and then the motor drives the liquid suction assembly to move to the upper part of the microfluidic device along the second lead screw for liquid addition. After each liquid addition, the liquid suction assembly moves upward to reset.

[0009] Preferably, a liquid suction head support and a medical waste box are arranged in the mounting rack. The liquid suction head support is used for placing unused liquid suction heads, and the upper end of the medical waste box is open for storing used liquid suction heads.

[0010] With the above structural design, the motor drives the liquid suction assembly to move above the medical waste box along the second lead screw. At this time, the movable plate is located below the limit block, and the slider of the first lead screw moves upward. With the cooperation of the limit block, the movable plate removes the liquid suction head. The liquid suction assembly needs to repeat adding different reagents into the microfluidic device multiple times. After the liquid suction device discards the used liquid suction head, it can install the next liquid suction head more quickly, saving time, eliminating the process of manually replacing the liquid suction head, and increasing the automation degree of the device.

[0011] Preferably, a fixing rack is placed in the heating tank. A plurality of through holes for fixing the microfluidic device are equidistantly arranged at the upper end of the fixing rack. The drain pipe at the tail of the microfluidic device passes through the through holes and extends out from the gap between the fixing rack and the heating tank. A water pipe extends into the heating tank, and the other end of the water pipe is connected to a water pump to inject hot water into the heating tank.

[0012] With the above structural design, during the experiment, the fixing rack is placed in the heating tank for use, so that when the microfluidic device is placed in the heating tank for heating, it can be inserted into the through holes on the fixing rack for support and fixation, increasing the stability of the placement of the microfluidic device and making the microfluidic device not prone to tipping. The warm water directly contacts the sleeve of the microfluidic device. Due to the large specific heat capacity of water, the heat conduction effect is better and the heating is more uniform.

[0013] Preferably, the heating tank is divided into an outer water bath tank and an inner water bath tank. The upper end of the inner water bath tank is fixedly connected with an extension plate, and the extension plate overlaps above the outer water bath tank. A heating device is installed on the outer side wall of the inner water bath tank, and a heat preservation layer is installed on the inner side wall of the outer water bath tank.

[0014] With the above structural design, the heating tank is set as a detachable outer water bath tank and inner water bath tank. On the one hand, it is convenient to install the heating device and the heat preservation layer. On the other hand, it is convenient to pour out the water in the heating tank after the experiment. A heat preservation layer is arranged in the outer water bath tank, which helps to slow down heat conduction and reduce the influence of the external temperature on the internal liquid. The inner water bath tank is provided with a heating device to improve the heating effect and make the heating of the heating tank more uniform.

[0015] Compared with the prior art, the beneficial effects of the present utility model are:

[0016] 1. The moving component drives the liquid suction component to move in the horizontal and vertical directions, enabling the liquid suction component to automatically extract and transfer reagents, improving the efficiency of reagent picking and placing, and solving the problems of cumbersome and laborious manual operation and low efficiency.

[0017] 2. A heat preservation layer is arranged in the outer water bath tank, which helps to slow down heat conduction and reduce the influence of the external temperature on the internal liquid. A heating device is arranged in the inner water bath tank to improve the heating effect, making the heating of the heating tank more uniform, enhancing the reaction activity of the reagent, and improving the experimental efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 is a schematic structural diagram of the present utility model;

[0019] Figure 2 is a schematic structural diagram of the liquid suction component of the present utility model;

[0020] Figure 3 is a schematic structural diagram of the liquid suction device of the present utility model;

[0021] Figure 4 is a schematic structural diagram of the heating tank of the present utility model;

[0022] Figure 5 is a schematic internal structural diagram of the heating tank of the present utility model. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0023] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0024] Please refer to Figures 1-5, the present utility model provides a technical solution: a CTC enrichment staining auxiliary device, comprising: a heating tank 10, which is located in the mounting frame 1 and is used to provide a constant temperature environment for the reagent in the microfluidic device 15; a liquid suction assembly 6, which is used to suck the reagent from the test tube 20 and add it to the microfluidic device 15 in the heating tank 10; a moving assembly 2, which is arranged on the mounting frame 1 and is used to drive the liquid suction assembly 6 to move in the horizontal and vertical directions; the mounting frame 1 is fixedly connected to the base 22 with a rectangular plate structure, the liquid suction assembly 6 includes a limit frame 601 and several liquid suction devices 5 installed inside the limit frame 601, the limit frame 601 is a metal plate with a C-shaped cross-section, a movable plate 603 is arranged at the lower end of the limit frame 601, the movable plate 603 is connected to the movable plate 603 through a return spring 602, the lower end of the liquid suction device 5 passes through the limit frame 601 and the movable plate 603 and is sleeved with a liquid suction head 9, and two limit blocks 7 are fixedly connected to the relative positions on the inner side surface of the mounting frame 1, and the movable plate 603 is located below the limit blocks 7. By driving the liquid suction assembly 6 to move in the horizontal and vertical directions through the moving assembly 2, the liquid suction assembly 6 can automatically extract and transfer the reagent, improving the efficiency of reagent picking and placing, and solving the problems of cumbersome and laborious manual operation and low efficiency.

[0025] The moving assembly 2 includes a second lead screw 201 and a slide rail 202 arranged in parallel at the upper end of the mounting frame 1, a slider one threadedly connected to the second lead screw 201 and a slider two slidably connected to the slide rail 202 are fixedly connected to both ends of the cross beam 3, a first lead screw 203 is fixedly connected to the middle position of the cross beam 3, the first lead screw 203 is arranged vertically, and the limit frame 601 is slidably connected to the first lead screw 203. By driving the cross beam 3 to move through the second lead screw 201, the liquid suction assembly 6 is driven to move horizontally, and the liquid suction assembly 6 is driven to move vertically through the second lead screw 201.

[0026] The liquid aspirator 5 includes a sleeve end 501 and a connection end 502 fixedly connected from bottom to top. The connection end 502 passes through the limit frame 601 and is fixedly connected with a fixing bolt 503. The bottom end of the sleeve end 501 cooperates with the liquid suction head 9. A vacuum pipeline is arranged along the length direction of the liquid aspirator 5. One end of the pipeline opens at the sleeve end 501, and the other end of the pipeline opens at the fixing bolt 503 and is connected to a vacuum pump through a communicating pipe 8. A compression spring 504 is sleeved on the connection end 502, and both ends of the compression spring 504 are abutted against the sleeve end 501 and the limit frame 601 respectively. As the slider of the first lead screw 203 of the liquid suction assembly 6 moves downward, the sleeve end 501 gradually approaches the liquid suction head bracket 19 below until the sleeve end 501 is in close fit with the liquid suction head 9. At this time, the compression spring 504 plays a buffering role. After the liquid suction head 9 is clamped at the lower end of the liquid aspirator 5, the slider of the first lead screw 203 moves upward, and the liquid suction head 9 moves upward with the liquid aspirator 5, realizing the automatic clamping operation of the liquid aspirator 5 to the liquid suction head 9. The motor drives the liquid suction assembly 6 to move along the second lead screw 201 above the reagent kit. The slider of the first lead screw 203 drives the liquid suction assembly 6 to move downward into the reagent kit. The liquid suction head 9 extends into the test tube 20. The negative pressure assembly is turned on to suck the reagent. After the reagent is sucked up, the first lead screw 203 drives the liquid suction assembly 6 to move upward and reset, and then the motor drives the liquid suction assembly 6 to move along the second lead screw 201 above the microfluidic device 15 for liquid addition. After each liquid addition is completed, the liquid suction assembly 6 moves upward and resets.

[0027] A liquid suction head bracket 19 and a medical waste box 21 are arranged in the mounting bracket 1. The liquid suction head bracket 19 is used to place unused liquid suction heads 9. The upper end of the medical waste box 21 is open and is used to store used liquid suction heads 9. The motor drives the liquid suction assembly 6 to move along the second lead screw 201 above the medical waste box 21. At this time, the movable plate 603 is located below the limit block 7. The slider of the first lead screw 203 moves upward. With the cooperation of the limit block 7, the movable plate 603 removes the liquid suction head 9. The liquid suction assembly 6 needs to repeat adding different reagents into the microfluidic device 15 multiple times. After the liquid aspirator 5 discards the used liquid suction head 9, it can install the next liquid suction head 9 more quickly, saving time, eliminating the process of manually replacing the liquid suction head 9, and increasing the automation degree of the device.

[0028] A fixing frame 12 is placed in the heating tank 10, and a plurality of through holes 2001 for fixing the microfluidic device 15 are equidistantly provided on the upper end of the fixing frame 12, and a discharge pipe 16 at the tail of the microfluidic device 15 passes through the through hole 2001 and extends from the gap between the fixing frame 12 and the heating tank 10, and a water pipe 23 is inserted into the interior of the heating tank 10, and a water pump is connected to the other end of the water pipe 23 to inject hot water into the heating tank 10. During the experiment, the fixing frame 12 is placed in the heating tank 10 for use, so that the microfluidic device 15 can be inserted into the through hole 2001 on the fixing frame 12 for support and fixation when placed in the heating tank 10 for heating, thereby increasing the stability of the placement of the microfluidic device 15 and preventing the microfluidic device 15 from tipping over. The warm water directly contacts the sleeve of the microfluidic device 15, and due to the large specific heat capacity of water, the heat conduction effect is better and the heating is more uniform.

[0029] The heating tank 10 is a hollow structure, in which a heating device 18 and a heat-insulating layer 17 are arranged. The hollow structure effectively slows down the speed at which the temperature inside the heating tank 10 is transferred to the outside, thereby achieving a heat-insulating effect.

[0030] The heating tank 10 is divided into an outer water bath 101 and an inner water bath 102. The upper end of the inner water bath 102 is fixedly connected with an extension plate 1021, and the extension plate 1021 is overlapped on the top of the outer water bath 101. The heating device 18 is installed on the outer side wall of the inner water bath 102, and the insulation layer 17 is installed on the inner side wall of the outer water bath 101. The heating tank 10 is set as a detachable outer water bath 101 and inner water bath 102. On the one hand, it is convenient to install the heating device 18 and the insulation layer 17, and on the other hand, it is convenient to pour out the water in the heating tank 10 after the experiment is completed. The insulation layer 17 is set in the outer water bath 101, which helps to slow down the heat conduction and reduce the influence of the external temperature on the internal liquid. The inner water bath 102 is provided with a heating device 18 to improve the heating effect and make the heating of the heating tank 10 more uniform.

[0031] A temperature sensor 14 is installed on the inner wall of the inner water bath 102, and a display panel 13 is provided outside the heating tank 10 to receive the electrical signal of the temperature sensor 14. The temperature sensor 14 can monitor the temperature inside the heating tank 10 and display it through the display panel 13 to remind the experimenter.

[0032] Working principle: Before the liquid suction assembly 6 sucks the reagent each time, the liquid suction head 9 needs to be installed first. After one kind of reagent is injected, it is necessary to remove the liquid suction head 9 and install a new one. The liquid suction assembly 6 moves downward along with the slider of the first lead screw 203, and the sleeve end 501 gradually approaches the liquid suction head 9 placement rack below until the sleeve end 501 is in close fit with the liquid suction head 9. At this time, the compression spring 504 plays a buffering role. After the liquid suction head 9 is clamped at the lower end of the liquid suction device 5, the slider of the first lead screw 203 moves upward, and the liquid suction head 9 moves upward with the liquid suction device 5, realizing the automatic latching operation of the liquid suction device 5 with the liquid suction head 9. The motor drives the liquid suction assembly 6 to move along the second lead screw 201 above the reagent kit. The slider of the first lead screw 203 drives the liquid suction assembly 6 to move downward into the reagent kit. The liquid suction head 9 extends into the test tube 20. The vacuum pump is turned on to suck the reagent. After the reagent is sucked, the first lead screw 203 drives the liquid suction assembly 6 to move upward and reset. Then the motor drives the liquid suction assembly 6 to move along the second lead screw 201 above the microfluidic device 15 for liquid addition. After each liquid addition, the liquid suction assembly 6 moves upward and resets. The motor drives the liquid suction assembly 6 to move along the second lead screw 201 above the medical waste box 21. At this time, the movable plate 603 is located below the limit block 7. The slider of the first lead screw 203 moves upward. With the cooperation of the limit block 7, the movable plate 603 removes the liquid suction head 9. The liquid suction assembly 6 needs to repeat the operation of adding different reagents into the microfluidic device 15 for multiple times. After the liquid suction device 5 discards the used liquid suction head 9, it can install the next liquid suction head 9 more quickly.

[0033] In this embodiment, the temperature is set to 37 degrees. The water pump is turned on to inject water into the heating tank 10. After the water injection is completed, the water pump is turned off. The experimenter can determine the water temperature according to the display panel 13 set on the heating tank 10 during the experiment, and then control the temperature and keep it warm by controlling the number of heating tapes installed inside the heating tank 10.

[0034] The content not described in detail in this specification belongs to the prior art well-known to those skilled in the art. Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A CTC enrichment and staining auxiliary device, characterized in that: include: A heating tank (10), the heating tank (10) being located in the mounting frame (1) and used to provide a constant temperature environment for the reagents in the microfluidic device (15); A liquid aspiration component (6), used for aspirating reagents from the test tube (20) and adding the reagents to the microfluidic device (15) in the heating tank (10); A moving assembly (2), the moving assembly (2) being arranged on the mounting frame (1) and being used for driving the liquid suction assembly (6) to move in horizontal and vertical directions; The liquid pipetting assembly (6) comprises a limit frame (601) and a plurality of liquid pipettes (5) installed inside the limit frame (601); a movable plate (603) is arranged at the lower end of the limit frame (601); the movable plate (603) is connected to the movable plate (603) via a reset spring (602); the lower end of the liquid pipette (5) passes through the limit frame (601) and the movable plate (603) and is sleeved with a liquid pipette head (9); two limit blocks (7) are fixedly connected to the relative positions of the inner side surface of the mounting frame (1); the movable plate (603) is located below the limit block (7); The moving assembly (2) comprises a second lead screw (201) and a slide rail (202) which are arranged in parallel at the upper end of the mounting frame (1); the second lead screw (201) and the upper ends of the slide rail (202) are slidably connected to a crossbeam (3); a first lead screw (203) is fixedly connected to the middle position of the crossbeam (3); the first lead screw (203) is arranged vertically, and the limit frame (601) is slidably connected to the first lead screw (203).

2. A CTC enrichment and staining auxiliary device according to claim 1, characterized in that: The pipette (5) comprises, from bottom to top, a fixedly connected sleeve end (501) and a connecting end (502); the connecting end (502) passes through the limiting frame (601) and is fixedly connected to a fixing bolt (503); the bottom end of the sleeve end (501) cooperates with the pipette head (9); a vacuum pipe is arranged along the length direction of the pipette (5); one end of the pipe opens at the sleeve end (501); the other end of the pipe opens at the fixing bolt (503) and is connected to the vacuum pump through a connecting pipe (8); a compression spring (504) is sleeved on the connecting end (502); the two ends of the compression spring (504) are respectively in contact with the sleeve end (501) and the limiting frame (601).

3. A CTC enrichment and staining auxiliary device according to claim 1, characterized in that: The mounting frame (1) is provided with a pipette head support (19) and a medical waste box (21); the pipette head support (19) is used to place unused pipette heads (9); the medical waste box (21) has an opening at the upper end and is used to store used pipette heads (9).

4. A CTC enrichment and staining auxiliary device according to claim 1, characterized in that: A fixing frame (12) is placed in the heating tank (10), and a plurality of through holes (2001) for fixing the microfluidic device (15) are equidistantly provided at the upper end of the fixing frame (12), and a discharge pipe (16) at the tail of the microfluidic device (15) passes through the through hole (2001) and extends out from the gap between the fixing frame (12) and the heating tank (10), and a water pipe (23) extends into the interior of the heating tank (10), and the other end of the water pipe (23) is connected to a water pump for injecting hot water into the heating tank (10).

5. A CTC enrichment and staining auxiliary device according to claim 1, characterized in that: The heating tank (10) is divided into an outer water bath tank (101) and an inner water bath tank (102); an extension plate (1021) is fixedly connected to the upper end of the inner water bath tank (102); the extension plate (1021) is overlapped on the upper part of the outer water bath tank (101); a heating device (18) is installed on the outer side wall of the inner water bath tank (102); and an insulation layer (17) is installed on the inner side wall of the outer water bath tank (101).

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