Integrated card for wet sheet microscopic examination and dry chemical joint detection

Abstract

The utility model discloses a wet sheet microscopic examination and dry chemistry combined detection integrated card which comprises a detection card body formed by buckling a bottom plate and a cover plate, a microscopic examination groove, a dyeing groove and a sample groove are formed in the bottom plate, and through holes in one-to-one correspondence with the microscopic examination groove, the dyeing groove and the sample groove are formed in the cover plate; the groove bottom of the microscopic examination groove is provided with a light hole, a microscopic examination slide is adaptively clamped in the microscopic examination groove, the microscopic examination slide is formed by bonding a glass slide and a cover glass, the middle part of the microscopic examination slide is provided with a crack cavity, and the cover glass is provided with a sample adding port and an exhaust port which are communicated with the crack cavity. The combined detection card has the advantages that the combined detection card integrating wet sheet microscopic examination and dry chemical detection is formed, wet sheet microscopic examination consumables and dry chemical detection consumables do not need to be prepared respectively, the detection difficulty and the labor intensity of experimenters are reduced, the detection efficiency is improved, and the detection cost is reduced. And development and application of integrated equipment for automatically completing wet sheet microscopic examination and dry chemical detection are facilitated.

Description

Technical Field

[0001] The utility model relates to the technical field of medical inspection, in particular to an integrated card for wet mount microscopy and dry chemistry combined detection. Background Art

[0002] At present, the conventional detection methods for gynecological vaginitis and male prostatitis mainly include wet mount microscopy and dry chemistry detection. The combination of the two can improve the clinical coincidence rate. The wet mount microscopy method is to smear the processed specimen on a glass slide, cover it with a coverslip, and then observe, analyze and judge under a microscope; while the dry chemistry detection is a joint detection card that can simultaneously detect multiple related indicators of gynecological vaginitis or male prostatitis. During actual operation, only the processed specimen needs to be dropped into the corresponding card holes, and then a visible color change will occur. In the existing practical applications, although both wet mount microscopy and dry chemistry detection are used, combined detection cannot be achieved, and different detection consumables need to be prepared, resulting in a cumbersome operation process, a relatively high overall labor intensity, and a very low detection efficiency. Summary of the Invention

[0003] The purpose of the utility model is to provide an integrated card for wet mount microscopy and dry chemistry combined detection in view of the defects of the prior art.

[0004] To achieve the above purpose, the utility model can adopt the following technical solutions:

[0005] The integrated card for wet mount microscopy and dry chemistry combined detection of the utility model includes a detection card body formed by buckling a bottom plate and a cover plate; a microscopy groove, a staining groove and a sample groove are arranged on the bottom plate, and through holes corresponding to the microscopy groove, the staining groove and the sample groove are opened on the cover plate; wherein, a light-transmitting hole is opened at the bottom of the microscopy groove, and a microscopy glass slide is adaptively placed in the microscopy groove. The microscopy glass slide is formed by bonding a glass slide and a coverslip, and has a slit cavity in the middle. A sample adding port and an exhaust port communicated with the slit cavity are opened on the coverslip.

[0006] Specifically, there is at least one sample groove; the bottom of the microscopy groove is a slope structure with a gradually increasing height from one side to the other side; the sample adding port is a semi-circular opening opened at one end of the coverslip, the exhaust port is a strip-shaped opening opened at the other end of the coverslip, the arc end of the sample adding port faces the exhaust port, and a blocking strip located on the top surface of the coverslip is arranged near the arc end of the semi-circular opening.

[0007] Furthermore, an anti-fooling chamfer can be arranged at a corner of the detection card body, which can effectively prevent the wrong direction during use.

[0008] The advantages of the present utility model are its delicate design, compact structure, simple operation, and convenience for batch processing. By reasonably arranging a microscopic examination groove, a staining groove, and a sample groove on the bottom plate, and opening through holes corresponding to them on the cover plate, and placing a microscopic examination slide in the microscopic examination groove, a combined detection card integrating wet-mounted microscopic examination and dry chemical detection is formed, eliminating the need to separately prepare consumables for wet-mounted microscopic examination and dry chemical detection, reducing the detection difficulty and the labor intensity of experimental personnel, improving the detection efficiency, and contributing to the development and application of an integrated device for automatically completing wet-mounted microscopic examination and dry chemical detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0009] Figure 1 is a schematic structural diagram of the present utility model.

[0010] Figure 2 is Figure 1 an exploded view of

[0011] Figure 3 is Figure 2 an exploded view of the microscopic examination slide in DETAILED DESCRIPTION OF THE EMBODIMENTS

[0012] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0013] It should be noted that all directional indications (such as up, down, left, right, front, back...) in the embodiments of the present utility model are only used to explain the relative positional relationship and movement conditions between components in a specific posture (as shown in the drawings). If the specific posture changes, the directional indications will also change accordingly.

[0014] As Figures 1-3 shown, the wet-mounted microscopic examination and dry chemical combined detection integrated card of the present utility model includes a detection card body, which is formed by buckling a bottom plate 1 with a rectangular structure and a cover plate 2; in order to effectively adapt to an automated detection instrument and prevent misplacement during use, an anti-fooling chamfer is also provided at a corner of the detection card body, enabling the detection instrument to automatically detect whether the direction of the detection card body is incorrect.

[0015] A microscopic examination groove 3, a staining groove 4, and at least one sample groove 5 are provided on the bottom plate 1. The microscopic examination groove 3 and the staining groove 4 are arranged on one side of the bottom plate 1, while the sample groove 5 is arranged on the other side of the bottom plate 1; at the same time, through holes 6 corresponding to the microscopic examination groove 3, the staining groove 4, and the sample groove 5 are also opened on the cover plate 2.

[0016] Specifically, the microscopy slot 3 is used to complete the wet mount microscopy operation. A microscopy slide 7 is adaptively placed therein, and a light-transmitting hole 8 for facilitating light transmission during microscopy observation is provided on the bottom wall of the microscopy slot 3. The microscopy slide 7 is formed by adhesively bonding a glass slide 7.1 and a coverslip 7.2 up and down. A thin groove cavity is provided on the top surface of the glass slide 7.1. Thus, when the glass slide 7.1 and the coverslip 7.2 are fixedly bonded together, a gap cavity 7.3 can be formed between the glass slide 7.1 and the coverslip 7.2. At this time, a sample addition port 7.4 and an exhaust port 7.5 communicating with the gap cavity 7.3 are provided on the coverslip 7.2. Thus, the capillary principle can be utilized to automatically suck the sample liquid injected into the sample addition port 7.4 into the gap cavity 7.3 to complete the preparation of the microscopy slide 7. Among them, the sample addition port 7.4 is a semi-circular structural opening provided at one end of the coverslip 7.2, and the exhaust port 7.5 is a strip-shaped structural opening provided at the other end of the coverslip 7.2. The arc end of the sample addition port 7.4 faces the exhaust port 7.5, which can make the sample liquid injected therein fill the gap cavity 7.3 more uniformly and quickly. In addition, in order to prevent the sample liquid from flowing into the exhaust port 7.5 from the top surface of the coverslip 7.2, a stop bar 7.6 should also be provided on the top surface of the coverslip 7.2. The stop bar 7.6 is located near the arc end of the sample addition port 7.4 and can effectively block the sample liquid injected into the sample addition port 7.4. Further, the bottom of the microscopy slot 3 should also be a slope structure with a gradually increasing height from one side to the other side. When placing the microscopy slide 7, the sample addition port 7.4 is located at the lower end of the slope, and the exhaust port 7.5 is located at the upper end of the slope. Thus, when sucking the sample liquid injected into the sample addition port 7.4 by using the capillary principle, the sample liquid has an upward climbing flow state in the gap cavity 7.3, which can prevent the generation of bubbles during the microscopy slide preparation process.

[0017] The staining slot 4 is used in conjunction with the wet mount microscopy and is used to complete the staining operation of the sample liquid. The staining slot 4 is an empty slot, and only when in use, a staining solution and the sample liquid are injected into the staining slot 4 for mixing, and then the stained sample liquid is sucked and injected into the sample addition port 7.4 of the microscopy slide 7 to complete the slide preparation.

[0018] The sample cell 5 is used to complete dry chemical detection, and solid dry chemical detection reagents are coated therein. Common sample cells 5 include one-hole, four-hole, six-hole, and eight-hole ones; the one-hole sample cell 5 belongs to a chlamydia combined detection card, and α-mannosidase is coated in the hole; the four-hole sample cell 5 belongs to a mycoplasma combined detection card, and proline aminopeptidase, leucine aminopeptidase, glucosidase, and sialidase are respectively coated in the holes; the six-hole sample cell 5 belongs to a prostatitis combined detection card, and pH value, leukocyte esterase, lecithin body, citric acid, zinc, and PAP are respectively coated in the holes; the eight-hole sample cell 5 belongs to a gynecology combined detection card, and hydrogen peroxide, leukocyte esterase, coagulase, proline aminopeptidase, sialidase, N-acetylglucosaminidase, glucosidase, and pH value are respectively coated in the holes.

[0019] The integrated card for the whole wet mount microscopy and dry chemical combined detection is exquisitely designed, with a compact structure and simple operation, facilitating batch processing; through the reasonable arrangement of the microscopy cell 3, staining cell 4, and sample cell 5, a combined detection card integrating wet mount microscopy and dry chemical detection is formed, eliminating the need to separately prepare wet mount microscopy consumables and dry chemical detection consumables, reducing the detection difficulty and the labor intensity of experimental personnel, improving the detection efficiency, and contributing to the development and application of an integrated device for automatically completing wet mount microscopy and dry chemical detection.

Claims

1. A wet mount microscopy and dry chemical combined detection integrated card, comprising a detection card body formed by buckling a bottom plate and a cover plate; characterized in that: A microscopic examination slot, a staining slot and a sample slot are arranged on the bottom plate, and through holes corresponding to the microscopic examination slot, the staining slot and the sample slot are opened on the cover plate; wherein a light-transmitting hole is opened at the bottom of the microscopic examination slot, and a microscopic examination glass slide is placed in the microscopic examination slot in an adapter card, the microscopic examination glass slide is formed by gluing a glass slide and a cover glass, and has a slit cavity in the middle, and a sample addition port and an exhaust port connected to the slit cavity are opened on the cover glass.

2. The wet mount microscopy and dry chemistry combined detection integrated card according to claim 1, characterized in that: There is at least one sample slot.

3. The wet mount microscopy and dry chemistry combined detection integrated card according to claim 1, characterized in that: The bottom of the mirror inspection groove is a slope structure with a height gradually increasing from one side to the other side.

4. The wet mount microscopy and dry chemistry combined detection integrated card according to claim 1, characterized in that: The sample loading port is a semicircular opening opened at one end of the cover glass, and the exhaust port is a strip opening opened at the other end of the cover glass. The arc end of the sample loading port faces the exhaust port, and a baffle located on the top surface of the cover glass is arranged near the arc end of the semicircular opening.

5. The wet mount microscopy and dry chemistry combined detection integrated card according to claim 1, characterized in that: A fool-proof chamfer is provided at one corner of the detection card body.

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