Arc-shaped perfusion needle device

Abstract

The arc-shaped perfusion needle device can be used for mouse common bile duct puncture, the arc-shaped perfusion needle device comprises a puncture part and an injection part, the puncture part comprises a puncture needle, the puncture needle comprises a puncture section, the puncture section is of a bent structure, the degree of adaptation between the bent structure and the walking angle of the mouse common bile duct is high, and puncture needle insertion is easy to succeed. The injection part comprises an injector, an infusion tube and a filter, one end of the infusion tube is detachably connected with the injector, the other end of the infusion tube is connected with the filter, the puncture part is arranged at the end, not connected with the infusion tube, of the filter, the injector is used for containing liquid to be injected, and the filter is used for filtering impurities in the liquid to be injected entering the puncture part. The arc-shaped perfusion needle device can reduce the technical difficulty of a common bile duct puncture method for primary pancreas islet extraction, increase the success rate of the common bile duct puncture method and guarantee the yield of primary pancreas islet extraction.

Description

Technical Field

[0001] The utility model relates to the technical field of medical products, in particular to an arc-shaped perfusion needle device. Background Art

[0002] Islet isolation mainly includes three main steps: pancreatic digestive enzyme perfusion, islet cell isolation, and islet cell purification. Pancreatic perfusion is the primary step, and whether the pancreatic duct is filled with collagenase is the key to subsequent isolation and purification. In the current pancreatic perfusion method, an injection needle is used for puncture, but it is difficult to grasp the angle between the injection needle and the common bile duct of a mouse. A straight injection needle is very likely to penetrate the common bile duct and lead to failure. In addition, when injecting collagenase into the pancreas, if there are impurities in it, the injection needle and the common bile duct may be blocked, resulting in puncture failure.

[0003] Therefore, there is an urgent need for an arc-shaped perfusion needle device to solve the above problems. Summary of the Utility Model

[0004] The purpose of the utility model is to provide an arc-shaped perfusion needle device, which can reduce the technical difficulty of the common bile duct puncture method for primary islet extraction, increase the success rate of the common bile duct puncture method, and ensure the yield of primary islet extraction.

[0005] As such a concept, the technical solution adopted by the utility model is as follows:

[0006] Provide an arc-shaped perfusion needle device suitable for puncturing the common bile duct of a mouse. The arc-shaped perfusion needle device includes:

[0007] A puncture part, including a puncture needle, and the puncture needle includes a puncture section, and the puncture section is a curved structure;

[0008] An injection part, including a syringe, an infusion tube, and a filter. One end of the infusion tube is detachably connected to the syringe, and the other end is connected to the filter. The puncture part is arranged at the end of the filter that is not connected to the infusion tube. The syringe is used to hold the liquid to be injected, and the filter is used to filter impurities in the liquid to be injected entering the puncture section.

[0009] Optionally, the puncture needle further includes a connection section, the connection section is a straight structure, one end of the connection section is connected to the filter, and the other end is connected to the first end of the puncture section. The included angle between the tangent line of the first end and the connection section is α, and 150° ≤ α ≤ 170°.

[0010] Optionally, the radius of curvature of the puncture section is R1, and 850 mm ≤ R1 ≤ 950 mm.

[0011] Optionally, the length of the puncture section is L1, and 7 mm ≤ L1 ≤ 9 mm.

[0012] Optionally, the length of the connecting section is L2, where 3 mm ≤ L2 ≤ 5 mm.

[0013] Optionally, the length of the infusion tube is L3, where 90 mm ≤ L3 ≤ 110 mm.

[0014] Optionally, a plurality of filter holes are provided on the filter, and the diameter of the filter holes is d, where 75 μm ≤ d ≤ 85 μm.

[0015] Optionally, the puncturing part further includes a needle sleeve, and the needle sleeve can be sleeved on the puncturing needle.

[0016] Optionally, the puncturing needle is made of stainless steel.

[0017] Optionally, the injection part is made of PVC material.

[0018] The beneficial effects of the present utility model are as follows:

[0019] The arc-shaped perfusion needle device proposed by the present utility model can be used for puncturing the common bile duct of mice. The arc-shaped perfusion needle device includes a puncturing part and an injection part. The puncturing part includes a puncturing needle, and the puncturing needle includes a puncturing section. The puncturing section is a curved structure, and the adaptability of the curved structure to the running angle of the common bile duct of mice is relatively high, and the puncturing needle insertion is relatively easy to succeed. The injection part includes a syringe, an infusion tube, and a filter. One end of the infusion tube is detachably connected to the syringe, and the other end is connected to the filter. The end of the filter that is not connected to the infusion tube is provided with the puncturing part. The syringe is used to accommodate the liquid to be injected, and the filter is used to filter impurities in the liquid to be injected into the puncturing part. The non-freshly prepared collagenase solution or improper operation during the preparation process may cause impurities in the collagenase, and it is difficult to ensure that all consumables are disposable in actual operation. After multiple punctures, it is inevitable that some tissue fragments will remain in the puncturing needle, and during injection, they may be sucked back due to siphon or other reasons. The presence of the filter can prevent tissue fragments from flowing back into the infusion tube and the syringe, avoiding contamination of the collagenase and resulting in puncture failure. In addition, if an obstruction occurs during the injection process, it can be quickly checked whether the puncturing needle is blocked or the infusion tube is blocked, and then some components can be replaced or dredged. The setting of the infusion tube and the detachable connection between the infusion tube and the filter enable direct removal of the syringe to aspirate collagenase even when there is not enough collagenase during the perfusion process, without the need to remove the entire arc-shaped perfusion needle device to aspirate collagenase and then repeat puncturing the mice. The arc-shaped perfusion needle device can reduce the technical difficulty of the common bile duct puncture method for primary islet extraction, increase the success rate of the common bile duct puncture method, and ensure the yield of primary islet extraction. Description of the Drawings

[0020] Figure 1 is a schematic structural diagram of the arc-shaped perfusion needle device provided by an embodiment of the present utility model;

[0021] Figure 2 It is a schematic structural diagram of the puncture needle provided by the embodiment of the present utility model;

[0022] Figure 3 It is a schematic structural diagram of the filter provided by the embodiment of the present utility model;

[0023] Figure 4 It is a schematic structural diagram of the infusion tube provided by the embodiment of the present utility model.

[0024] In the figure:

[0025] 1. Puncture needle; 11. Puncturing section; 12. Connecting section; 2. Needle head sleeve; 3. Filter; 4. Infusion tube; 5. Syringe. Specific embodiments

[0026] In order to make the technical problems solved by the present utility model, the technical solutions adopted and the achieved technical effects clearer, the technical solutions of the present utility model will be further described below with reference to the accompanying drawings and through specific embodiments. It can be understood that the specific embodiments described herein are only used to explain the present utility model, rather than limiting the present utility model. In addition, it should be noted that for the sake of description, only the parts related to the present utility model are shown in the drawings, rather than all of them.

[0027] In the description of the present utility model, unless otherwise clearly defined and limited, the terms "connected", "connected" and "fixed" shall be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or integrated; it can be a mechanical connection or an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, and it can be the internal communication of two components or the interaction relationship between two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific situations.

[0028] In the present utility model, unless otherwise clearly defined and limited, the first feature being "above" or "below" the second feature may include the direct contact between the first and second features, or may include the situation where the first and second features are not in direct contact but in contact through other features between them. Moreover, the first feature being "above", "above" and "on" the second feature includes that the first feature is directly above and obliquely above the second feature, or simply means that the horizontal height of the first feature is higher than that of the second feature. The first feature being "below", "below" and "under" the second feature includes that the first feature is directly below and obliquely below the second feature, or simply means that the horizontal height of the first feature is lower than that of the second feature.

[0029] In the description of this embodiment, the orientation or positional relationships such as "upper", "lower", "left", "right", etc. are based on the orientation or positional relationships shown in the drawings. They are only for the convenience of description and simplifying the operations, rather than indicating or implying that the device or component referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation to the present utility model. In addition, the terms "first" and "second" are only used for distinction in description and have no special meanings.

[0030] The technical solution of the present utility model will be further described below in conjunction with the drawings and through specific embodiments.

[0031] As Figures 1 to 4 shown, this embodiment provides an arc-shaped perfusion needle device, which can be used for puncturing the common bile duct of mice. The arc-shaped perfusion needle device includes a puncture part and an injection part. The puncture part includes a puncture needle 1, and the puncture needle 1 includes a puncture section 11. The puncture section 11 is a curved structure, and the degree of adaptation of the curved structure to the running angle of the common bile duct of mice is relatively high, and it is relatively easy to succeed in puncturing the needle. The injection part includes a syringe 5, an infusion tube 4, and a filter 3. One end of the infusion tube 4 is detachably connected to the syringe 5, and the other end is connected to the filter 3. The end of the filter 3 that is not connected to the infusion tube 4 is provided with the puncture part. The syringe 5 is used to accommodate the liquid to be injected, and the filter 3 is used to filter the impurities in the liquid to be injected into the puncture part. The non-freshly prepared collagenase solution or improper operation during the preparation process may cause impurities in the collagenase, and it is difficult to ensure that all consumables are used only once in actual operation. After multiple punctures, it may be difficult to avoid some tissue fragments remaining in the puncture needle 1. During injection, it may be sucked back due to siphon or other reasons. The presence of the filter 3 can prevent tissue fragments from flowing back into the infusion tube 4 and the syringe 5, and can avoid contaminating the collagenase and resulting in puncture failure. In addition, if an obstruction occurs during the injection process, it can be quickly checked whether the puncture needle 1 is blocked or the infusion tube 4 is blocked, and then some components can be replaced or dredged. The setting of the infusion tube 4 and the detachable connection between the infusion tube 4 and the filter 3 enable directly removing the syringe 5 to suck the collagenase even when there is not enough collagenase during the infusion process, without the need to remove the entire arc-shaped perfusion needle device to suck the collagenase and then repeat the puncture of the mice. The arc-shaped perfusion needle device provided in this embodiment can reduce the technical difficulty of the common bile duct puncture method for primary islet extraction, increase the success rate of the common bile duct puncture method, and ensure the yield of primary islet extraction.

[0032] During the specific implementation, 5 ml or slightly more than 5 ml of collagenase is firstly drawn into the syringe 5 and the air in the syringe 5 is emptied. Then, the syringe 5 is connected to the infusion tube 4, and the collagenase is injected through the infusion tube 4 to reach the needle tip of the puncture needle 1, a small amount of liquid is pushed out, and the bubbles visible to the naked eye are discharged out of the infusion tube 4. Finally, the infusion tube 4 is filled with collagenase and there are no bubbles visible to the naked eye. After the mouse is anesthetized, its limbs are fixed. After blood is collected from the heart, the abdominal cavity is cut open with ophthalmic scissors to fully expose the mouse's abdominal viscera. The point where the common bile duct joins the duodenum is accurately found below the liver. Normally, it is in the shape of a white dot. It is clamped with a vascular clamp, and then the common bile duct is bluntly separated with forceps. At the beginning of the common bile duct, the arc-shaped puncture needle 1 is slowly inserted into the common bile duct. The needle insertion depth is about 3mm to 5mm. Be careful to avoid puncturing the common bile duct. Slowly push the syringe 5 filled with collagenase connected to the infusion tube 4. When the head of the pancreas is filled, it indicates that the puncture is successful. Continue to inject collagenase until the tail of the pancreas is filled and stop the injection.

[0033] Optionally, the puncture needle 1 also includes a connecting section 12, which is a linear structure, one end of the connecting section 12 is connected to the filter 3, and the other end is connected to the first end of the puncture section 11, and the angle between the tangent of the first end and the connecting section 12 is α, 150°≤α≤170°. In specific implementation, the connecting section 12 is non-coaxially arranged with the central axis of the filter 3, so that the puncture needle 1 as a whole can be located at the center of the filter 3, which is convenient for puncture. In this embodiment, α=160°, that is, the angle between the puncture section 11 and the connecting section 12 is 160°, which not only takes into account the convenience of puncture, but also prevents the puncture needle 1 from puncturing the common bile duct of the mouse.

[0034] Optionally, the curvature radius of the puncture section 11 is R1, 850 mm ≤ R1 ≤ 950 mm. In this embodiment, the curvature radius of the puncture section 11 is 900 mm, which is better adapted to the curvature of the common bile duct of mice.

[0035] Optionally, the length of the puncture section 11 is L1, 7mm≤L1≤9mm. In this embodiment, the length of the puncture section 11 is 8mm. During the specific implementation, the puncture needle 1 slowly penetrates the common bile duct, and the needle insertion depth is about 3mm to 5mm. The 8mm puncture section 11 ensures the needle insertion depth and is also convenient for needle removal after puncture, which can prevent the needle from falling off after puncture.

[0036] Optionally, the length of the connecting section 12 is L2, 3mm≤L2≤5mm.

[0037] In this embodiment, the puncture needle 1 is made of stainless steel, and the outer diameter of the needle head of the puncture needle 1 is 0.2 mm.

[0038] Optionally, the length of the infusion tube 4 is L3, where 90 mm ≤ L3 ≤ 110 mm. In this embodiment, the length of the infusion tube 4 is 100 mm. The infusion tube 4 includes a connected main body section and a flared section. The length of the main body section is 90 mm, and the diameter of the main body section is 2 mm. The main body section is connected to the filter 3, while the flared section is connected to the syringe 5. The length of the flared section is 10 mm, and the diameter of the flared section is 3 mm to facilitate connection to the needle hub of the syringe 5.

[0039] Optionally, the filter 3 is provided with a plurality of filter holes, and the diameter of the filter holes is d, where 75 μm ≤ d ≤ 85 μm. The filter 3 can be a disposable medical filter, and the size of the filter 3 is suitable for filtering out impurities contained in the collagenase.

[0040] In this embodiment, the filter 3 is made of PVC (Polyvinyl chloride). The outer diameter of the filter 3 is 10 mm.

[0041] Optionally, as Figure 1 shown, the puncture part further includes a needle sleeve 2, and the needle sleeve 2 can be sleeved on the puncture needle 1. Specifically, during implementation, the puncture needle 1 is arranged on the filter 3, and the needle sleeve 2 is sleeved on the puncture needle 1, and the needle sleeve 2 is in interference fit with the filter 3 to achieve the fixation of the needle sleeve 2 and other components of the arc-shaped perfusion needle device, protect the puncture needle 1, and isolate bacteria.

[0042] The above embodiments only illustrate the basic principles and characteristics of the present invention. The present invention is not limited by the above embodiments. Without departing from the spirit and scope of the present invention, there are various changes and modifications to the present invention, and these changes and modifications all fall within the scope of the present invention claimed. The scope of protection claimed by the present invention is defined by the appended claims and their equivalents.

Claims

1. An arc-shaped perfusion needle device, applicable to the puncture of the common bile duct of mice, characterized in that, The arc-shaped infusion needle device includes: A puncture part, including a puncture needle (1), and the puncture needle (1) includes a puncture section (11), and the puncture section (11) is a curved structure; An injection part, including a syringe (5), an infusion tube (4) and a filter (3). One end of the infusion tube (4) is detachably connected to the syringe (5), and the other end is connected to the filter (3). The puncture part is provided at the end of the filter (3) that is not connected to the infusion tube (4). The syringe (5) is used to accommodate the liquid to be injected, and the filter (3) is used to filter impurities in the liquid to be injected that enters the puncture section (11).

2. The arc-shaped perfusion needle device according to claim 1, characterized in that, The puncture needle (1) further includes a connection section (12), the connection section (12) is a straight structure, one end of the connection section (12) is connected to the filter (3), and the other end is connected to the first end of the puncture section (11). The included angle between the tangent of the first end and the connection section (12) is α, and 150° ≤ α ≤ 170°.

3. The arc-shaped perfusion needle device according to claim 1, characterized in that, The radius of curvature of the puncture section (11) is R1, and 850 mm ≤ R1 ≤ 950 mm.

4. The arc-shaped perfusion needle device according to claim 1, characterized in that, The length of the puncture section (11) is L1, and 7 mm ≤ L1 ≤ 9 mm.

5. The arc-shaped perfusion needle device according to claim 2, wherein The length of the connection section (12) is L2, and 3 mm ≤ L2 ≤ 5 mm.

6. The arc-shaped perfusion needle device according to claim 1, wherein The length of the infusion tube (4) is L3, and 90 mm ≤ L3 ≤ 110 mm.

7. The arc-shaped perfusion needle device according to claim 1, wherein, A plurality of filter holes are provided on the filter (3), and the diameter of the filter holes is d, and 75 μm ≤ d ≤ 85 μm.

8. The arc-shaped perfusion needle device according to claim 1, characterized in that, The puncture part further includes a needle head sleeve (2), and the needle head sleeve (2) can be sleeved on the puncture needle (1).

9. The arc-shaped perfusion needle device according to any one of claims 1 to 8, characterized in that The puncture needle (1) is made of stainless steel.

10. The arc-shaped perfusion needle device according to any one of claims 1 to 8, characterized in that, The injection part is made of PVC material.

Images