Stem cell culture device with replaceable culture medium

Abstract

The stem cell culture device is characterized by comprising a porous culture dish arranged in the middle, a new liquid storage device and a new liquid filling device which are arranged on one side of the porous culture dish, and a waste liquid storage device and a waste liquid extraction device which are arranged on the other side of the porous culture dish, the porous culture dish is filled with a culture medium, mesenchymal stem cells or embryonic stem cells or induced pluripotent stem cells are cultured in the porous culture dish, the culture medium is stored in the new liquid storage device, and the culture medium comprises at least one of collagen, fibronectin and laminin; the waste liquid extraction device is used for extracting the invalid culture medium from the porous culture dish into the waste liquid storage device; the new liquid filling device is used for extracting the culture medium in the new liquid storage device and filling the culture medium into the porous culture dish. According to the scheme, safe replacement of the stem cell culture medium is achieved, and practical value and economic value are achieved.

Description

Technical Field

[0001] This application relates to the fields of cell engineering, bioengineering, and tissue engineering equipment, and particularly relates to a stem cell culture device with replaceable culture medium. Background Art

[0002] Tissue engineering is an interdisciplinary field of biology, engineering, and chemistry. Its basic concept is to create functional tissues through cells, engineering methods, and appropriate biomaterials to replace damaged or dysfunctional tissues. In the process of realizing the above functions, the culture of stem cells is a key task. Stem cells absorb the nutrients required for growth from the culture device during the culture process and excrete metabolic wastes. To ensure the smooth culture of stem cells, the freshness of the culture medium and the excretion of wastes should be ensured as much as possible, but the stem cells in culture cannot be removed. How to smoothly replace the culture medium. Utility Model Content

[0003] (1) Technical Problem

[0004] In view of the above-mentioned prior art, the technical problem to be solved by this application is how to replace the culture medium during the culture process of stem cells.

[0005] (2) Technical Solution

[0006] In view of the above technical problems, this application proposes a stem cell culture device with replaceable culture medium, which is characterized in that it includes a porous culture dish arranged in the middle, a fresh liquid storage device and a fresh liquid filling device arranged on one side of the porous culture dish, and a waste liquid storage device and a waste liquid extraction device arranged on the other side of the porous culture dish; the porous culture dish is filled with a culture medium and is cultured with mesenchymal stem cells or embryonic stem cells or induced pluripotent stem cells, the fresh liquid storage device stores a culture medium, and the culture medium includes at least one of collagen, fibronectin, and laminin; the waste liquid extraction device extracts the expired culture medium from the porous culture dish into the waste liquid storage device; the fresh liquid filling device extracts the culture medium in the fresh liquid storage device and fills it into the porous culture dish.

[0007] Further, the fresh liquid filling device includes a first horizontal motor screw module arranged on a fixed frame, a first vertical motor screw module arranged on the slider of the first horizontal motor screw module, a first pipe system arranged on the slider of the first vertical motor screw module, and suction heads communicating with each culture hole on the porous culture dish are arranged on the first pipe system; the pipe system is connected to a pump.

[0008] Further, the waste liquid extraction device includes a second horizontal motor screw module disposed on a fixed frame, a second vertical motor screw module disposed on a slider of the second horizontal motor screw module, a second pipe system disposed on a slider of the second vertical motor screw module, and suction heads communicated with each culture hole on the porous culture dish corresponding to the second pipe system; the pipe system is connected to a pump.

[0009] Further, the downward stroke of the slider of the second vertical motor screw module makes the suction head extend into the culture hole but not touch the bottom of the culture hole.

[0010] Further, the downward stroke of the slider of the second vertical motor screw module enables the suction head to extend downward above the culture hole.

[0011] Further, the fresh liquid storage device includes an open cylinder from which all the suction heads on the first pipe system can suck the culture medium; the waste liquid storage device includes a waste liquid cylinder.

[0012] (III) Beneficial Effects

[0013] This solution realizes the safe replacement of the stem cell culture medium and has practical and economic value. Description of the Drawings

[0014] Figure 1 is the overall schematic diagram of the present application;

[0015] Figure 2 is the overall schematic diagram of the present application observed from another perspective;

[0016] Figure 3 is Figure 2 the schematic diagram of another working state in

[0017] Figure 4 is Figure 3 the schematic diagram of another aspect of the work in

[0018] Reference Signs

[0019] 1. Porous culture dish

[0020] 2. Fresh liquid storage device

[0021] 3. Waste liquid storage device

[0022] 4. Fresh liquid filling device

[0023] 5. Waste liquid extraction device

[0024] 6. Culture hole

[0025] 7. Open cylinder

[0026] 8. Waste liquid cylinder

[0027] 9. First horizontal motor lead screw module

[0028] 10. First vertical motor lead screw module

[0029] 11. First pipe system

[0030] 12. Suction head

[0031] 13. Second horizontal motor lead screw module

[0032] 14. Second vertical motor lead screw module

[0033] 15. Second pipe system

[0034] 16. Suction head

[0035] 17. Pump Detailed implementation manners

[0036] The following further describes the present utility model in conjunction with embodiments.

[0037] As Figure 1 shown, a stem cell culture device with replaceable culture medium according to the present application is characterized in that: it includes a porous culture dish 1 arranged in the middle, a fresh liquid storage device 2 and a fresh liquid filling device 4 arranged on one side of the porous culture dish, and a waste liquid storage device 3 and a waste liquid extraction device 5 arranged on the other side of the porous culture dish; the porous culture dish is filled with a culture medium and cultured with mesenchymal stem cells or embryonic stem cells or induced pluripotent stem cells, the fresh liquid storage device stores a culture medium, and the culture medium includes at least one of collagen, fibronectin and laminin; the waste liquid extraction device extracts the expired culture medium from the porous culture dish into the waste liquid storage device; the fresh liquid filling device extracts the culture medium in the fresh liquid storage device and fills it into the porous culture dish.

[0038] In the stem cell culture operation, the difficulty in replacing the culture medium lies in that it is impossible to ensure that the stem cells in culture are not sucked away when extracting the waste liquid. In this solution, components such as collagen are added to the culture dish. For adherent cultured stem cells, after absorbing the above nutrients, they adhere to the bottom of the porous culture dish. At this time, the waste liquid can be extracted and the fresh liquid can be filled.

[0039] Further, the fresh liquid filling device includes a first horizontal motor lead screw module 9 arranged on a fixed frame, a first vertical motor lead screw module 10 arranged on the slider of the first horizontal motor lead screw module, a first pipe system 11 arranged on the slider of the first vertical motor lead screw module, and suction heads communicating with each culture hole 6 on the porous culture dish corresponding to the first pipe system; the pipe system is connected to a pump.

[0040] Further, the waste liquid extraction device includes a second horizontal motor screw module 13 disposed on the fixed frame, a second vertical motor screw module 14 disposed on the slider of the second horizontal motor screw module, a second pipe system 15 disposed on the slider of the second vertical motor screw module, and suction heads communicating with each culture well 6 on the porous culture dish are provided on the second pipe system; the pipe system is connected to a pump.

[0041] Further, the downward stroke of the slider of the second vertical motor screw module causes the suction head to extend into the culture well but not touch the bottom of the culture well.

[0042] Further, the downward stroke of the slider of the second vertical motor screw module enables the suction head to extend downward above the culture well.

[0043] Further, the fresh liquid storage device includes an open cylinder, and all the suction heads on the first pipe system can suck the culture medium therefrom; the waste liquid storage device includes a waste liquid cylinder.

[0044] During use, when the culture medium needs to be replaced, first use the waste liquid extraction device to extend the suction head into the culture well through the second horizontal module and the second vertical module, and extract the culture medium eliminated above the stem cells adhered to the bottom of the culture well. Then, the two modules return to their original positions, and the extracted eliminated culture medium is discharged into the waste liquid cylinder. Then, the first horizontal module and the first vertical module suck the fresh culture medium in the fresh liquid cylinder and inject it into the culture well to complete the replacement of the culture medium. During the above process, the pump sucks or discharges through the pipe system.

[0045] The above has described in detail an embodiment of the present invention, but the above content is only a preferred embodiment of the present invention and cannot be considered as limiting the scope of implementation of the present invention. All equivalent changes and improvements made according to the scope of the present invention application should still fall within the patent coverage scope of the present invention.

Claims

1. A stem cell culture device with a replaceable culture medium, characterized in that: It includes a porous culture dish disposed in the middle, a fresh liquid storage device and a fresh liquid filling device disposed on one side of the porous culture dish, and a waste liquid storage device and a waste liquid extraction device disposed on the other side of the porous culture dish; the porous culture dish is filled with a culture medium and is culturing mesenchymal stem cells or embryonic stem cells or induced pluripotent stem cells, the fresh liquid storage device stores a culture medium, and the culture medium includes at least one of collagen, fibronectin and laminin; the waste liquid extraction device extracts the expired culture medium from the porous culture dish into the waste liquid storage device; The fresh liquid filling device extracts the culture medium in the fresh liquid storage device and fills it into the porous culture dish.

2. The stem cell culture device with a replaceable culture medium according to claim 1, characterized in that: The fresh liquid filling device includes a first horizontal motor screw module disposed on a fixed frame, a first vertical motor screw module disposed on the slider of the first horizontal motor screw module, a first pipe system disposed on the slider of the first vertical motor screw module, and suction heads communicating with each culture hole on the porous culture dish are provided corresponding to the first pipe system; the pipe system is connected to a pump.

3. The stem cell culture device with replaceable culture medium according to claim 2, characterized in that: The waste liquid extraction device includes a second horizontal motor screw module disposed on a fixed frame, a second vertical motor screw module disposed on the slider of the second horizontal motor screw module, a second pipe system disposed on the slider of the second vertical motor screw module, and suction heads communicating with each culture hole on the porous culture dish are provided corresponding to the second pipe system; the pipe system is connected to a pump.

4. The stem cell culture device with a replaceable culture medium according to claim 3, characterized in that: The downward stroke of the slider of the second vertical motor screw module makes the suction head extend into the culture hole but not touch the bottom of the culture hole.

5. The stem cell culture device with replaceable culture medium according to claim 4, characterized in that: The downward stroke of the slider of the second vertical motor screw module enables the suction head to extend downward above the culture hole.

6. The stem cell culture device with replaceable culture medium according to claim 5, characterized in that: The fresh liquid storage device includes an open cylinder, and all the suction heads on the first pipe system can suck the culture medium therefrom; the waste liquid storage device includes a waste liquid cylinder.

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