Raman spectrometer for antigen protein detection

Abstract

The utility model discloses a Raman spectrometer for antigen protein detection, which comprises a horizontally placed base, a sample table which is arranged on the base and moves along a straight line in the horizontal direction, and a laser which is arranged above the sample table and emits laser towards the sample table, the clamping groove is matched with a test strip dripped with a sample solution to be detected in size so as to accommodate the test strip; and the power device is used for controlling the sample table to move along a straight line and driving the sample table to move relative to the laser so as to ensure that the laser is focused on the specific position of the test strip on the sample table and realize Raman spectrum analysis on the specific position of the test strip. The Raman spectrometer is simple in structure, and errors caused by manual operation can be reduced, so that the use portability and the detection accuracy are both considered, and rapid and accurate detection of the virus antigen protein is realized.

Description

Technical Field

[0001] The utility model relates to the field of Raman spectrometers, and particularly to a Raman spectrometer for antigen protein detection. Background Art

[0002] After irradiating a sample with a laser, the scattered light whose propagation direction and frequency both change is called Raman scattering. Since the frequency difference between the scattered light and the incident light, that is, the Raman shift, is independent of the frequency of the incident light and only related to the structure of the sample molecules themselves. Different chemical bonds or different characteristic groups correspond to different Raman shifts. Therefore, through a Raman spectrometer, the molecular structure of a sample can be determined using Raman scattering.

[0003] Although traditional Raman spectrometers have high accuracy and sensitivity, the entire instrument structure is complex, has high requirements for the use site, and requires professional technical personnel to operate, and cannot be applied to scenarios that require rapid detection. In the prior art, although handheld Raman spectrometers have broken through the limitations of the site and professional technical personnel, the detection errors caused by humans are relatively large, and they cannot meet the needs of daily detection.

[0004] Therefore, the prior art still needs to be improved and developed. Content of the Utility Model

[0005] In view of the above deficiencies of the prior art, the purpose of the present utility model is to provide a Raman spectrometer for antigen protein detection, aiming to solve the problem that existing Raman spectrometers cannot balance portability and detection accuracy.

[0006] The technical solution of the present utility model is as follows:

[0007] The present utility model provides a Raman spectrometer for antigen protein detection, including: a horizontally placed base, a sample stage arranged on the base and moving linearly in the horizontal direction, and a laser arranged above the sample stage and emitting laser light towards the sample stage. The sample stage includes:

[0008] A card slot, the card slot is matched with the size of a test strip dropped with a sample solution to be measured to accommodate the test strip;

[0009] A power device, the power device controls the linear movement of the sample stage, drives the sample stage to move relative to the laser, so as to ensure that the laser focuses on a specific position of the test strip on the sample stage, and realizes Raman spectroscopy analysis of the specific position of the test strip.

[0010] In one embodiment, the power device includes:

[0011] A lead screw, the lead screw is horizontally arranged, and the sample stage moves along the lead screw through a slider;

[0012] A motor, which is connected to the lead screw and drives the lead screw to rotate so that the sample stage moves along the lead screw through the slider.

[0013] In one embodiment, the base includes:

[0014] A bottom plate, which is horizontally arranged to support the base;

[0015] A control device, which is fixed on the bottom plate and is communicatively connected to the laser, and receives the sampling signal of the laser to complete Raman spectroscopy analysis;

[0016] A substrate, which is parallel to the bottom plate and is arranged above the control device, and the sample stage is arranged on the substrate.

[0017] In one embodiment, vertical support columns are arranged between the bottom plate and the substrate, and the height of the support columns is greater than the height of the control device to ensure that there is a gap between the control device and the substrate.

[0018] In one embodiment, the base further includes:

[0019] A support device, which is vertically arranged at the edge of the substrate, and the laser is movably connected to the support device and is arranged above the card slot of the sample stage.

[0020] In one embodiment, the support device includes:

[0021] A slide rail, which is vertically arranged at the edge of the substrate;

[0022] A laser holder, which holds the laser on the laser holder, and the laser holder is slidably arranged along the slide rail to adjust the distance between the laser and the sample stage.

[0023] In one embodiment, the laser holder includes:

[0024] A bayonet, which holds the laser in the bayonet to realize the movable connection between the laser and the support device;

[0025] A holder slider, which is sleeved on the slide rail and moves along the slide rail to adjust the position of the laser holder on the slide rail.

[0026] In one embodiment, a tightening knob is arranged on the holder slider. Loosen the tightening knob to make the holder slider move along the slide rail, and tighten the tightening knob to fix the holder slider on the slide rail.

[0027] In one embodiment, the control device is communicatively connected to the motor to control the motor to drive the sample stage to move relative to the laser, so as to ensure that the laser is focused on a specific position of the test strip on the sample stage.

[0028] In one embodiment, the control device is further connected to a display device to provide a user interaction interface and output the results of Raman spectroscopy analysis.

[0029] Beneficial effects: The present utility model provides a Raman spectrometer for antigen protein detection, comprising: a horizontally placed base, a sample stage arranged on the base and moving linearly in the horizontal direction, and a laser arranged above the sample stage and emitting laser towards the sample stage. The sample stage includes: a card slot, the card slot being matched in size with a test strip dropped with a sample solution to be measured to accommodate the test strip; and a power device, the power device controlling the sample stage to move linearly, driving the sample stage to move relative to the laser, so as to ensure that the laser is focused on a specific position of the test strip on the sample stage, and realizing Raman spectroscopy analysis of the specific position of the test strip. The Raman spectrometer has a simple structure and can reduce the error of manual operation, thus taking into account the portability in use and the detection accuracy, and realizing the rapid and accurate detection of virus antigen proteins. Description of the Drawings

[0030] Figure 1 It is a perspective view of the Raman spectrometer for antigen protein detection in the embodiment of the present utility model.

[0031] Figure 2 It is an exploded view of the Raman spectrometer for antigen protein detection in the embodiment of the present utility model.

[0032] Figure 3 It is a right view of the Raman spectrometer for antigen protein detection in the embodiment of the present utility model.

[0033] Figure 4 It is a front view of the Raman spectrometer for antigen protein detection in the embodiment of the present utility model.

[0034] Figure 5 It is a schematic diagram showing the change of characteristic peak intensity with the distance of the laser on the Z-axis in the embodiment of the present utility model. Detailed Embodiments

[0035] The present utility model provides a Raman spectrometer for antigen protein detection. To make the purpose, technical solution and effects of the present utility model clearer and more definite, the following further details the present utility model. It should be understood that the specific embodiments described herein are only used to explain the present utility model and are not used to limit the present utility model.

[0036] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field to which this utility model belongs. The terms used in the description of this utility model herein are only for the purpose of describing specific embodiments and are not intended to limit this utility model.

[0037] It should be noted that the orientation or positional relationship indicated by terms such as "center", "upper", "lower", "left", "right", "inner", "outer", "vertical", "horizontal", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing this utility model and simplifying the description, rather than indicating or implying that the structure referred to must have a specific orientation or must be constructed in a specific orientation, and should not be construed as a limitation to this utility model.

[0038] Immunochromatography is a commonly used method for virus detection. Generally, the antigen protein of the virus is used as the target detection substance. After dropping the sample to be tested on the immunochromatography test strip, the target detection substance is captured by the sandwich immunoassay method, and then a preliminary judgment on the presence or absence of the antigen protein is formed based on the appearance of the test line and the control line on the immunochromatography test strip, and further to judge whether there is a virus in the sample to be tested. However, for the existing immunochromatography test strip, only the appearance of the test line and the control line can be judged by the naked eye, there are deviations in visual observation, resulting in low accuracy of the detection results. This utility model provides a Raman spectrometer for antigen protein detection, which uses the Raman spectrometer to detect the test strip dropped with the sample solution to be tested, and judges whether the sample to be tested contains antigen protein based on the Raman spectroscopy results at the test line and the control line, thus eliminating the error of visual judgment. The structure of the Raman spectrometer is simple, requires little space, and has high portability, so that both portability and detection accuracy can be taken into account.

[0039] This utility model provides a Raman spectrometer for antigen protein detection, as Figure 1 shown, the Raman spectrometer includes a horizontally placed base 100, a sample stage 200 arranged on the base and moving linearly in the horizontal direction, and a laser 300 arranged above the sample stage 200 and emitting laser light towards the sample stage 200. Among them, the moving direction of the sample stage 200 is set as the X-axis direction, that is, the sample stage 200 can only displace along the X-axis; the vertical direction is set as the Z-axis direction, that is, the laser light emitted by the laser 300 is focused on the sample stage 200 along the Z-axis direction. By adjusting the position of the sample stage 200 on the X-axis, the laser light emitted by the laser 300 is focused on a specific position of the sample stage 200 to complete the Raman spectroscopy analysis of the specific position on the sample stage 200.

[0040] Furthermore, as Figure 1As shown in the figure, the sample stage 200 includes a card slot 210 and a power device 220. The test strip with the solution to be measured is accommodated in the card slot 210, and the power device controls the sample stage 200 to move along the X-axis direction, driving the test strip accommodated in the card slot 210 on the sample stage 200 to move relative to the laser 300, so as to ensure that the laser emitted by the laser is focused on a specific position of the test strip on the sample stage 200, and Raman spectroscopy analysis of the specific position of the test strip is realized.

[0041] Specifically, the card slot 210 is arranged on the top surface of the sample stage 200 and faces the laser 300, so as to ensure that the laser emitted by the laser 300 is focused on the test strip in the card slot 210 when the test strip is accommodated in the card slot 210. Further, the size of the card slot 210 matches that of the test strip. After the test strip is placed in the card slot 210, when the sample stage 200 moves, the test strip moves synchronously with the sample stage 200, and the test strip and the sample stage 200 remain relatively stationary, so as to ensure that the moving distance of the test strip does not deviate, and the laser emitted by the laser 300 can be accurately focused on a specific position of the test strip, so as to perform Raman spectroscopy analysis on a specific position on the test strip.

[0042] Specifically, as Figure 4 shown in the figure, the power device 220 includes a lead screw 221, a slider 222 and a motor 223. The motor 223 is fixed on the base 100, the lead screw 221 is horizontally arranged and parallel to the X-axis direction. The motor 223 is connected to the lead screw 221, and the motor 223 drives the lead screw 221 to rotate. The sample stage 200 is sleeved on the lead screw 221 through the slider 222. When the motor 223 drives the lead screw 221 to rotate, it pushes the slider 222 to move along the lead screw 221, that is, the sample stage 200 moves along the lead screw 221 through the slider 222, so as to realize the movement of the sample stage 200 along the X-axis direction. By controlling the movement of the sample stage 200 through the motor 223, it is ensured that the moving distance of the sample stage 200 is accurately controllable, so as to ensure Raman spectroscopy analysis of a specific position of the test strip and reduce the deviation caused by manual operation. Further, by continuously driving the lead screw 221 to rotate through the motor 223 to drive the test strip on the sample stage 200 to continuously move, continuous sampling of an interval on the test strip by the laser 300 can be realized, and a stable detection result can be obtained.

[0043] Further, as Figure 4As shown, the base 100 includes a bottom plate 110, a control device 120, a substrate 130, and a support device 400. The bottom plate 110, the control device 120, and the substrate 130 are all horizontally arranged, and the support device 400 is arranged on one side of the substrate 130 and is vertically arranged. The sample stage 200 is arranged on the substrate 130, and the laser 300 is arranged on the support device 400, so as to ensure that the laser 300 is kept above the sample stage 200, and the laser 300 emits laser light towards the sample stage 200.

[0044] Specifically, as Figure 3 shown, the substrate 130 is arranged parallel to the bottom plate 110 and above the bottom plate 110, and the control device 120 is arranged between the substrate 130 and the bottom plate 110. Further, support columns 111 are provided between the bottom plate 110 and the substrate 130, and the height of the support columns 111 is greater than the height of the control device 120, so as to ensure that there is a gap between the control device 120 and the substrate 130, thereby avoiding squeezing the control device 120, facilitating heat dissipation of the control device 120, and prolonging the service life of the control device 120.

[0045] Optionally, the control device 120 is connected to the motor 223, and the working state of the motor 223 is controlled by the control device 120, so as to realize the movement control of the sample stage 200. Optionally, the control device 120 is a computer installed with control software, so as to ensure the precise control of the sample stage 200, realize routine characterization, avoid deviation of test results caused by human errors of operators, and improve the accuracy of the final detection results.

[0046] Optionally, the control device 120 is communicatively connected to the laser 300, receives the sampling signal of the laser 300 and completes Raman spectroscopy analysis, so as to realize routine characterization, avoid deviation of test results caused by human errors of operators, and improve the accuracy of the final detection results.

[0047] Optionally, the control device 120 is further connected to a display device, and the control device 120 provides a user interaction interface through the display device and outputs the results of Raman spectroscopy analysis, thereby reducing the operation difficulty of the Raman spectrometer and realizing the portable operation of the Raman spectrometer.

[0048] Specifically, as Figure 2As shown in the figure, the support device 400 includes a slide rail 410 vertically disposed at the edge of the substrate 130 and a laser holder 420 sleeved on the slide rail 410. The laser holder 420 can slide along the slide rail 410, that is, the slide rail 410 is disposed parallel to the Z-axis direction, and the position of the laser holder 420 in the Z-axis direction can be adjusted. Specifically, the laser 300 is clamped and connected to the laser holder 420, and the laser 300 can move along the slide rail 410 with the laser holder 420. That is, by adjusting the position of the laser holder 420 on the slide rail 410, the position of the laser 300 in the Z-axis direction can be adjusted, so as to adjust the distance between the laser 300 and the test strip on the sample stage 200. By adjusting the distance between the laser 300 and the test strip, the optimal Raman signal intensity can be found. As Figure 5 shown in the figure is the change in the characteristic peak intensity finally measured when the laser 300 moves in the Z-axis direction to change the distance between the laser 300 and the test strip in the Z-axis direction. Among them, when the distance is large, the distance between the laser and the sample stage is too far, resulting in defocusing of the light spot, thus affecting the final test result; when the distance is too small, the distance between the laser and the sample stage is too close, resulting in too high laser intensity and burning out the test strip, resulting in deviation of the test result and affecting the final detection result. Optionally, the distance between the laser and the test strip is adjusted to 1 cm. By adjusting the appropriate distance between the laser and the test strip, the optimal Raman signal intensity can be obtained, and the test strip can be prevented from being burned out due to too high laser intensity, so as to realize routine characterization, avoid deviation of the test result caused by human error of the operator, and improve the accuracy of the final detection result.

[0049] Furthermore, as Figure 2 shown in the figure, one side of the laser holder 420 is a holder slider 422, and a bayonet 421 is provided on the other side of the laser holder 420. The laser holder 420 is sleeved on the slide rail 410 through the holder slider 422, and the position of the laser holder 420 on the Z-axis is adjusted by sliding the holder slider 422 on the slide rail 410. Furthermore, the laser holder 420 is clamped and connected to the laser 300 through the bayonet 421, so as to ensure that the laser 300 is disposed above the sample stage 200, and the laser 300 moves along the slide rail 410 with the laser holder 420, so as to adjust the distance between the laser 300 and the test strip on the sample stage 200.

[0050] Optionally, a tightening knob 423 is provided at the position where the card holder slider 422 is sleeved on the slide rail 410 to adjust the frictional force between the card holder slider 422 and the slide rail 410. When the tightening knob 423 is tightened, the card holder slider 422 clamps the slide rail 410, thereby fixing the laser card holder 420 on the slide rail 410 to ensure the fixed position of the laser 300. When the tightening knob 423 is loosened, the card holder slider 422 can slide freely on the slide rail 410, thereby driving the laser card holder 420 to move to the required position on the slide rail 410 to adjust the distance between the laser 300 and the test strip on the sample stage 200.

[0051] Optionally, as Figure 2 and Figure 3 shown, the laser 300 includes a laser body 310 and a laser emitter 320. The size of the laser emitter 320 is smaller than the inner diameter of the bayonet 421, and the laser body 310 is larger than the inner diameter of the bayonet 421. The laser emitter 320 is arranged vertically downward through the bayonet 421 from above to clamp the laser 300 to the laser card holder 420 through the bayonet 421. Further, a USB interface is provided on the laser body 310 to communicate with the computing device, so as to control the operation of the laser 300 through the computing device, realize the stable output of the laser, and ensure the stability and reliability of the spectral detection result.

[0052] The application method of the Raman spectrometer for antigen protein detection according to the present invention will be described below with reference to specific embodiments.

[0053] First, a test strip needs to be prepared. The test strip includes a sample pad, a conjugate pad, a nitrocellulose membrane, and an absorbent pad connected in sequence. A test line and a control line are provided on the nitrocellulose membrane, and the test line is close to the conjugate pad, and the control line is close to the absorbent pad. Prepare nanoparticles modified with Raman reporter molecules and antibodies of specific antigen proteins as probe molecules, drop the solution of the probe molecules on the conjugate pad, drop the test sample solution on the sample pad to obtain a test strip with the test sample solution dropped thereon, and place the test strip in the card slot 210 of the sample stage 200.

[0054] Turn on the laser 300 and adjust the position of the laser holder 420 on the slide rail 410 to adjust the distance between the laser 300 and the test strip to 1 cm. Then, drive the lead screw 221 to rotate through the motor 223, so as to drive the sample stage 200 to move along with the slider 222 sleeved on the lead screw 221, and move until the laser emitted by the laser 300 is focused on the test line of the test strip. The control device 120 receives the sampling signal of the laser 300, completes the Raman spectroscopy analysis of the test strip at the test line position, and transmits the result of the Raman spectroscopy analysis to the computing device for storage. If the characteristic peak of the Raman reporter molecule modified in the probe molecule appears in the Raman spectrum at the test line position of the test strip, it indicates that the antigen protein is present in the sample to be tested. Through the full-automatic processing of the Raman spectrometer, standardized operation is achieved, making the final output result of the spectrum stable and reliable, effectively avoiding the errors of manual operation, and improving the accuracy of the final detection result.

[0055] Optionally, by controlling the motor 223, the control device 120, and the laser 300 through the computing device, continuous sampling of the area near the test line of the test strip can be achieved. Specifically, the sample stage 200 is controlled to move at a constant speed through the motor 223, and at every preset time interval, the sample stage 200 stops moving for the laser 300 to sample a certain point on the test strip until the laser 300 completes the continuous test of the test strip, thereby reducing the interference of background noise. Through the full-automatic processing of the Raman spectrometer, the continuous adjustment of the detection interval is achieved, making the final output result of the spectrum stable and reliable, effectively avoiding the errors of manual operation, and improving the accuracy of the final detection result.

[0056] In summary, the present invention provides a Raman spectrometer for antigen protein detection, including: a horizontally placed base, a sample stage arranged on the base and moving linearly in the horizontal direction, and a laser arranged above the sample stage and emitting laser towards the sample stage. The sample stage includes: a card slot, the card slot is matched with the size of the test strip dropped with the sample solution to be tested to accommodate the test strip; and a power device, the power device controls the sample stage to move linearly, driving the sample stage to move relative to the laser to ensure that the laser is focused on a specific position of the test strip on the sample stage, realizing the Raman spectroscopy analysis of the specific position of the test strip. The Raman spectrometer has a simple structure and can reduce the errors of manual operation, thus taking into account the portability and detection accuracy in use, and realizing the rapid and accurate detection of virus antigen proteins.

[0057] It should be understood that the application of the present utility model is not limited to the above examples. For those of ordinary skill in the art, improvements or transformations can be made according to the above description, and all such improvements and transformations shall fall within the protection scope of the appended claims of the present utility model.

Claims

1. A Raman spectrometer for antigen protein detection, characterized in that, Comprising: a horizontally placed base, a sample stage disposed on the base and moving linearly in the horizontal direction, and a laser disposed above the sample stage and emitting laser towards the sample stage. The sample stage includes: A card slot, which is sized to match the test strip with the test sample solution dropped thereon to accommodate the test strip; A power device, which controls the linear movement of the sample stage, driving the sample stage to move relative to the laser, so as to ensure that the laser focuses on a specific position of the test strip on the sample stage, realizing Raman spectroscopic analysis of the specific position of the test strip.

2. The Raman spectrometer for antigen protein detection according to claim 1, characterized in that, The power device includes: A lead screw, which is horizontally arranged, and the sample stage moves along the lead screw through a slider; A motor, which is connected to the lead screw and drives the lead screw to rotate to realize the movement of the sample stage along the lead screw through the slider.

3. The Raman spectrometer for antigen protein detection according to claim 2, wherein The base includes: A bottom plate, which is horizontally arranged to support the base; A control device, which is fixed on the bottom plate and communicatively connected to the laser, receiving the sampling signal of the laser to complete Raman spectroscopic analysis; A substrate, which is parallel to the bottom plate and disposed above the control device, and the sample stage is disposed on the substrate.

4. The Raman spectrometer for antigen protein detection according to claim 3, wherein, Support columns are vertically arranged between the bottom plate and the substrate, and the height of the support columns is greater than the height of the control device to ensure a gap is left between the control device and the substrate.

5. The Raman spectrometer for antigen protein detection according to claim 3, characterized in that, The base further includes: A support device, which is vertically arranged at the edge of the substrate, and the laser is movably connected to the support device and disposed above the card slot of the sample stage.

6. The Raman spectrometer for antigen protein detection according to claim 5, characterized in that, The support device includes: A slide rail, which is vertically arranged at the edge of the substrate; A laser holder, which holds the laser on the laser holder, and the laser holder is slidably arranged along the slide rail to adjust the distance between the laser and the sample stage.

7. The Raman spectrometer for antigen protein detection according to claim 6, characterized in that, The laser holder includes: A bayonet, which holds the laser in the bayonet to realize the movable connection between the laser and the support device; A holder slider, which is sleeved on the slide rail and moves along the slide rail to adjust the position of the laser holder on the slide rail.

8. The Raman spectrometer for antigen protein detection according to claim 7, characterized in that, A tightening knob is provided on the holder slider. Loosen the tightening knob to enable the holder slider to move along the slide rail, and tighten the tightening knob to fix the holder slider on the slide rail.

9. The Raman spectrometer for antigen protein detection according to claim 3, characterized in that, The control device is communicatively connected to the motor to control the motor to drive the sample stage to move relative to the laser, so as to ensure that the laser focuses on a specific position of the test strip on the sample stage.

10. The Raman spectrometer for antigen protein detection according to claim 3, wherein The control device is further connected to a display device to provide a user interaction interface and output the result of Raman spectroscopic analysis.

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