Protein precipitation supernate drainage device

Abstract

The utility model relates to the field of protein purification, and discloses a protein precipitation supernate drainage device, a precipitation tank is mounted in a mounting cylinder, the upper half part of the precipitation tank is of a cylindrical structure, the inner diameter of the lower end of the precipitation tank is gradually reduced to form a conical part, a pipeline is welded at the bottom end of the conical part, and the lower end of the pipeline is provided with a liquid outlet. A valve is installed below the pipeline, a cover plate is rotationally installed above the centrifugal machine, the cover plate penetrates through the upper portion and the lower portion of the cover plate and is slidably connected with a liquid suction pipe in a sleeving mode, and a liquid suction hose is connected to the end, away from the sedimentation tank, of the liquid suction pipe and connected with a liquid inlet of an external peristaltic pump. The settling tank is fixed in the mounting cylinder, and the settling tank is combined with the centrifugal machine, so that the settling tank can be centrifuged after isoelectric point settling, secondary transfer of protein precipitate after settling is not needed, the loss is reduced, and the convenience is improved.

Description

Technical Field

[0001] The utility model relates to the field of protein purification, in particular to a device for discharging the supernatant of protein precipitation. Background Art

[0002] In the process of protein purification, isoelectric precipitation is a commonly used method. It utilizes the property that the solubility of proteins is the lowest at the isoelectric point for separation. After isoelectric precipitation, it is a common operation step to collect protein precipitation by centrifugation. Generally, the operation is to first perform isoelectric precipitation on the protein, and then transfer the precipitate to a centrifuge for centrifugal precipitation. During the transfer, it is necessary to collect the precipitate, which makes the operation relatively complex, and some of the precipitate will adhere to the precipitation tank, resulting in the loss of the precipitate. Therefore, we have designed a device for discharging the supernatant of protein precipitation. Summary of the Utility Model

[0003] To solve the technical problem that it is necessary to collect the precipitate during the transfer, which makes the operation relatively complex, and some of the precipitate will adhere to the precipitation tank, resulting in the loss of the precipitate, the utility model provides a device for discharging the supernatant of protein precipitation.

[0004] The utility model is realized by adopting the following technical solutions: A device for discharging the supernatant of protein precipitation includes a centrifuge. A high-speed turntable is rotatably arranged inside the centrifuge. An installation cylinder is installed on the high-speed turntable. A precipitation tank is installed inside the installation cylinder. The upper half of the precipitation tank is in a cylindrical structure, and the inner diameter of the lower end of the precipitation tank gradually shrinks to form a conical part. A pipeline is welded to the bottom end of the conical part, and a valve is installed below the pipeline;

[0005] A cover plate is rotatably installed above the centrifuge. A liquid suction pipe passes through the cover plate vertically and is slidably sleeved with the cover plate. The end of the liquid suction pipe away from the precipitation tank is connected with a liquid suction hose, and the liquid suction hose is connected with the liquid inlet of an external peristaltic pump.

[0006] As a further improvement of the above solution, four groups of uniformly distributed support columns are arranged on the high-speed turntable of the centrifuge. A lower flange is welded to the top end of the installation cylinder. The lower flange is connected to the support column by screws, and the bottom end of the installation cylinder is fixed to the high-speed turntable by screws, which can quickly complete the installation and disassembly of the installation cylinder.

[0007] As a further improvement of the above solution, an upper flange is welded to the top end of the precipitation tank. The upper flange overlaps above the lower flange. A plurality of annularly distributed threaded sleeves are welded below the lower flange, and the upper flange and the lower flange are fixed by screws. The precipitation tank is sleeved inside the installation cylinder, and the upper flange overlaps above the lower flange, and the round holes between them correspond. Then, the screws are screwed into the threaded sleeves through the screw holes to complete its installation and facilitate subsequent disassembly.

[0008] As a further improvement of the above solution, a support cover with a cylindrical structure is arranged at the inner bottom end of the installation cylinder. The upper end of the support cover is widened to form a widened part. The inner wall of the widened part is inclined, and the outer surface of the conical part of the precipitation tank is in contact with the inner wall of the widened part, which can support the lower end of the precipitation tank. The setting of the widened part increases the contact area with the precipitation tank, improves the support effect, and makes it more stable.

[0009] As a further improvement of the above solution, a thickened disk is formed by thickening the lower end of the cover plate. The size of the thickened disk matches the inner diameter of the precipitation tank. When the cover plate is closed, the thickened disk is tightly pressed against the top of the precipitation tank. The thickened disk can be pressed above the precipitation tank, and then a sealing structure can be formed, so that the internal suspended matter will not leak during high-speed centrifugation.

[0010] As a further improvement of the above solution, a round hole is opened at the upper end of the cover plate and penetrates downward through the thickened disk. A sleeve pipe is welded in the round hole. The upper half of the sleeve pipe is located above the cover plate. A threaded hole is arranged on the side of the sleeve pipe, and a bolt is threadedly connected in the threaded hole. The liquid suction pipe is placed inside the precipitation tank through the sleeve pipe and can move up and down, thereby changing the height placed in the precipitation tank, and then the bolt can be tightened to fix it.

[0011] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0012] 1. By arranging an installation cylinder in the centrifuge and fixing the precipitation tank in the installation cylinder, the precipitation tank is combined with the centrifuge, so that centrifugation operation can be carried out after isoelectric point precipitation, without secondary transfer of the precipitated protein precipitate, reducing loss and improving convenience;

[0013] 2. By setting the bottom end of the precipitation tank in a conical structure, it can be used to precipitate protein precipitates, making the stratification of the supernatant and protein precipitates clearer. With the liquid suction pipe, liquid suction hose and peristaltic pump arranged, the supernatant can be drained, greatly reducing the centrifugation volume and shortening the centrifugation time. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 is a schematic diagram of the overall structure of a protein precipitate supernatant drainage device provided by the present utility model;

[0015] Figure 2 is Figure 1 the front view of

[0016] Figure 3 is Figure 1 the structural schematic diagram of the installation cylinder and the precipitation tank in

[0017] Figure 4 is Figure 2 schematic diagram of the internal structure.

[0018] Main symbol description:

[0019] 1. Centrifuge; 11. High-speed turntable; 12. Cover plate; 2. Support column; 3. Installation cylinder; 31. Lower flange; 4. Support cover; 41. Widened part; 5. Precipitation tank; 51. Conical part; 52. Upper flange; 6. Liquid suction pipe; 61. Sleeve pipe; 7. Liquid suction hose. Specific implementation mode

[0020] Next, in combination with the accompanying drawings and specific implementation modes, the present utility model will be further described. It should be noted that, on the premise of no conflict, the following-described embodiments or technical features can be combined arbitrarily to form new embodiments.

[0021] Embodiment:

[0022] Please combine Figures 1-4 , a protein precipitation supernatant liquid drainage device of this embodiment includes a centrifuge 1. A high-speed turntable 11 is rotatably arranged inside the centrifuge 1. An installation cylinder 3 is installed on the high-speed turntable 11. A precipitation tank 5 is installed inside the installation cylinder 3. The upper half of the precipitation tank 5 has a cylindrical structure, and the inner diameter of the lower end of the precipitation tank 5 gradually contracts to form a conical part 51. A pipeline is welded to the bottom end of the conical part 51, and a valve is installed below the pipeline;

[0023] A cover plate 12 is rotatably installed above the centrifuge 1. A liquid suction pipe 6 passes through and is slidably sleeved through the cover plate 12 up and down. One end of the liquid suction pipe 6 away from the precipitation tank 5 is connected to a liquid suction hose 7, and the liquid suction hose 7 is connected to the liquid inlet of an external peristaltic pump.

[0024] Four groups of uniformly distributed support columns 2 are arranged on the high-speed turntable 11 of the centrifuge 1. A lower flange 31 is welded to the top end of the installation cylinder 3. The lower flange 31 is connected to the support column 2 by screws, and the bottom end of the installation cylinder 3 is fixed to the high-speed turntable 11 by screws, which can quickly complete the installation and disassembly of the installation cylinder 3.

[0025] An upper flange 52 is welded to the top end of the precipitation tank 5. The upper flange 52 overlaps above the lower flange 31. A plurality of annularly distributed threaded sleeves are welded below the lower flange 31. The upper flange 52 and the lower flange 31 are fixed by screws. The precipitation tank 5 is sleeved inside the installation cylinder 3, and the upper flange 52 overlaps above the lower flange 31. The circular holes between them correspond. Then, the screws are screwed into the threaded sleeves through the screw holes to complete its installation and facilitate subsequent disassembly.

[0026] At the inner bottom end of the installation cylinder 3, there is a support cover 4 with a cylindrical structure. The upper end of the support cover 4 is widened to form a widened part 41. The inner wall of the widened part 41 is inclined, and the outer surface of the inner wall of the widened part 41 is in contact with the conical part 51 of the sedimentation tank 5, which can support the lower end of the sedimentation tank 5. Moreover, the setting of the widened part 41 increases the contact area with the sedimentation tank 5, improves the support effect, and makes it more stable.

[0027] At the lower end of the cover plate 12, a thickened disc is formed after thickening. The size of the thickened disc matches the inner diameter of the sedimentation tank 5. When the cover plate 12 is closed, the thickened disc is tightly pressed against the top of the sedimentation tank 5. The thickened disc can be pressed on the sedimentation tank 5, and then a sealing structure can be formed. During high-speed centrifugation, the internal suspended matter will not leak.

[0028] A round hole is opened at the upper end of the cover plate 12, and the round hole penetrates downward through the thickened disc. A sleeve pipe 61 is welded in the round hole. The upper half of the sleeve pipe 61 is located above the cover plate 12. A threaded hole is provided on the side of the sleeve pipe 61, and a bolt is threadedly connected in the threaded hole. The liquid suction pipe 6 is placed inside the sedimentation tank 5 through the sleeve pipe 61, and it can move up and down, thereby changing the height in the sedimentation tank 5. Then, after tightening the bolt, it can be fixed.

[0029] The implementation principle of a protein precipitation supernatant drainage device in the embodiment of the present application is as follows: In actual use, first fix the sedimentation tank 5 inside the installation cylinder 3, and then place the protein in the sedimentation tank 5. At this time, the protein can be precipitated by the isoelectric point precipitation method. Since the bottom end of the sedimentation tank 5 is provided with a conical part 51 and the inner diameter is small, the protein will precipitate at the bottom end of the conical part 51, and the supernatant will be separated from it. At this time, the sleeve pipe 61 can be moved downward and extended below the supernatant and 3 - 5 cm above the protein precipitate, and then connect the peristaltic pump. Then, the supernatant can be discharged and collected through the liquid suction pipe 6 and the liquid suction hose 7. At this time, the supernatant remaining in the sedimentation pipe is reduced, and the overall volume is decreased. Then, move the liquid suction pipe 6 upward and start the centrifuge 1. The sedimentation tank 5 can rotate at the same height, and then the protein precipitate can be centrifuged and purified;

[0030] By setting the installation cylinder 3 inside the centrifuge 1 and fixing the sedimentation tank 5 inside the installation cylinder 3, the sedimentation tank 5 is combined with the centrifuge 1, so that centrifugation can be carried out after isoelectric point precipitation without secondary transfer of the precipitated protein precipitate, reducing the loss and improving the convenience;

[0031] By setting the bottom end of the sedimentation tank 5 in a conical structure, it can be used to precipitate the protein precipitate, making the stratification of the supernatant and the protein precipitate clearer. With the cooperation of the liquid suction pipe 6, the liquid suction hose 7 and the peristaltic pump, the supernatant can be drained, greatly reducing the centrifugation volume and shortening the centrifugation time.

[0032] The above embodiments are only the preferred embodiments of the present utility model, and cannot be used to limit the scope of protection of the present utility model. Any non-substantive changes and substitutions made by those skilled in the art based on the present utility model fall within the scope of protection required by the present utility model.

Claims

1. A protein precipitation supernatant liquid discharging device, comprising a centrifuge (1), wherein a high-speed turntable (11) is rotatably arranged inside the centrifuge (1), and is characterized in that, An installation cylinder (3) is installed on the high-speed turntable (11). A precipitation tank (5) is installed inside the installation cylinder (3). The upper half of the precipitation tank (5) is of a cylindrical structure, and the inner diameter of the lower end of the precipitation tank (5) gradually contracts to form a conical part (51). A pipe is welded to the bottom end of the conical part (51), and a valve is installed below the pipe. A cover plate (12) is rotatably installed above the centrifuge (1). A liquid suction pipe (6) passes through the cover plate (12) vertically and is slidably sleeved thereon. One end of the liquid suction pipe (6) away from the precipitation tank (5) is connected to a liquid suction hose (7), and the liquid suction hose (7) is connected to the liquid inlet of an external peristaltic pump.

2. The protein precipitation supernatant liquid discharging device according to claim 1, wherein Four uniformly distributed support columns (2) are arranged on the high-speed turntable (11) of the centrifuge (1). A lower flange (31) is welded to the top end of the installation cylinder (3). The lower flange (31) is connected to the support column (2) by screws, and the bottom end of the installation cylinder (3) is fixed to the high-speed turntable (11) by screws.

3. The protein precipitation supernatant liquid discharging device according to claim 1, characterized in that, An upper flange (52) is welded to the top end of the precipitation tank (5). The upper flange (52) overlaps above the lower flange (31). A plurality of annularly distributed threaded sleeves are welded below the lower flange (31), and the upper flange (52) and the lower flange (31) are fixed by screws.

4. A protein precipitation supernatant liquid discharging device according to claim 1, characterized in that, A support cover (4) of a cylindrical structure is arranged at the bottom end inside the installation cylinder (3). The upper end of the support cover (4) is widened to form a widened part (41). The inner wall of the widened part (41) is inclined, and the inner wall of the widened part (41) contacts the outer surface of the conical part (51) of the precipitation tank (5).

5. The protein precipitation supernatant liquid discharging device according to claim 1, characterized in that The lower end of the cover plate (12) is thickened to form a thickened disc. The size of the thickened disc matches the inner diameter of the precipitation tank (5). When the cover plate (12) is closed, the thickened disc is tightly pressed against the top of the precipitation tank (5).

6. The protein precipitation supernatant liquid discharging device according to claim 5, wherein, A round hole is opened at the upper end of the cover plate (12), and the round hole penetrates downward through the thickened disc. A sleeve pipe (61) is welded in the round hole. The upper half of the sleeve pipe (61) is located above the cover plate (12). A threaded hole is arranged on the side surface of the sleeve pipe (61), and a bolt is threadedly connected in the threaded hole.

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