Milk cow mastitis bacterial liquid concentration detection device

Abstract

The utility model discloses a dairy cow mastitis bacterial liquid concentration detection device. The device comprises a propelling puncture assembly, a bacterial liquid concentration treatment bin and a test paper detection reaction cup, the propelling puncturing assembly comprises a piston handle and a small puncturing tip; the bacterial liquid concentration treatment bin comprises a sample loading bin, a sample recovery bin, a reaction tank, a concentrated liquid guide column, a bacterial liquid filter and a central sealing film; the small puncture tip is opposite to the central sealing film; the test paper detection reaction cup is arranged on the outer wall of the bacterial liquid concentration treatment bin, a clamping groove and a detection test strip mounted in the clamping groove are arranged in the test paper detection reaction cup, and the bottom of the test paper detection reaction cup is communicated with the reaction tank. According to the device, bacteria liquid concentration and detection reaction are integrated, various bacteria can be detected at the same time, and omission is avoided to the greatest extent; meanwhile, the process from milk sample collection to result reading can be completed within 20 min, the detection time is greatly shortened, early discovery and early treatment of mastitis can be truly achieved, and selection of antibacterial drugs is effectively guided.

Description

Technical Field

[0001] The utility model relates to the technical field of dairy cow mastitis detection devices, in particular to the concentration and detection of relevant bacterial liquids in milk. Background Technique

[0002] Dairy cow mastitis has always been a headache for farmers. This disease often reduces milk production and milk quality, not only reducing the economic income of farmers, but also posing a potential threat to human health. Controlling the occurrence of dairy cow mastitis is of extremely important significance for enhancing the added value of the dairy cow breeding industry and promoting the sustainable development of the dairy cow breeding industry.

[0003] Mastitis is divided into acute mastitis, chronic mastitis and occult mastitis. Acute mastitis is often characterized by breast swelling, heat sensation, induration and pain, and granular flocculants, pus and blood are occasionally seen in the milk. Chronic mastitis often evolves from the incomplete elimination of acute mastitis treatment, with relatively mild symptoms. There are lumps in the breast, the color of the milk becomes light, and small particles are occasionally suspended in it, and the milk production is significantly reduced. Occult mastitis usually has no obvious clinical manifestations, and there is no obvious change in the appearance of the milk, but lesions can be detected by physical, chemical or pathological methods, and it often turns into obvious clinical mastitis.

[0004] Chronic mastitis and occult mastitis often delay treatment due to untimely clinical detection, leading to further deterioration of the condition. Therefore, early detection and diagnosis are particularly important. Common methods for diagnosing mastitis include: clinical observation diagnosis method, somatic cell counting method, physical and chemical experiment diagnosis method, microbial culture and identification method, genomics detection method, and diagnostic test strip detection method. Each detection method has its own disadvantages. Among them, the clinical observation method is only applicable to acute mastitis; somatic cell counting requires professional instruments and the detection cost is relatively high; the physical and chemical experiment diagnosis method is easily affected by factors such as feed ratio, parity, and lactation time, and the physical and chemical properties vary greatly; the microbial culture and identification method has a large workload, is cumbersome to operate, and the detection time is relatively long, usually taking 3 days to complete, and it is easily affected by subjective judgment with a high false positive rate; the genomics detection method requires professional instruments and professional personnel to operate. Relatively speaking, the diagnostic test strip detection method is easy to operate, and the result reading is fast and intuitive, which is favored by farmers. The drawback is that as a primary screening product, the detection sensitivity is greatly affected by the content of pathogenic bacteria in the sample. When the content of pathogenic bacteria in the sample is low, it is easy to miss detection. Therefore, conventional milk sample enrichment operations are often used in the market, and the detection results can be obtained in about 6 - 7 hours. Compared with the conventional culture method, which takes about 1 - 5 days to obtain results, although the enrichment operation accelerates the detection process to a certain extent, for some urgent or suspected important cases, 6 - 7 hours is still too long. At the same time, considering that the milking frequency in the dairy farm is 2 - 3 times a day, and each milking takes 2 - 3 hours. Before the next milking, if mastitis can be diagnosed in time, it can ensure the quality and composition of the milk collected from the farm. Therefore, it is best to control the diagnostic time within 5 hours, and the shorter the overall time, the more convenient. Therefore, the overall detection time of the existing technology still does not meet the expectations, and it is particularly important to develop a detection method with shorter time, simple operation, and accurate results.

[0005] In addition, the pathogenic bacteria causing cow mastitis include Gram-positive bacteria such as Staphylococcus aureus and Streptococcus, and Gram-negative bacteria such as Klebsiella pneumoniae, Escherichia coli, Salmonella, Pasteurella, and Brucella. However, the disadvantage of the existing technology is that it can only detect Gram-positive bacteria and cannot identify Gram-negative bacteria, so it will cause missed detection of clinical mastitis. Utility Model Content

[0006] The utility model can simultaneously detect Gram-positive bacteria and Gram-negative bacteria and distinguish different colonies, which can achieve the most comprehensive detection without omission; at the same time, the unique bacterial liquid concentration device can complete the process from milk sample collection to result reading within 20 minutes, greatly shortening the detection time, and can truly achieve early detection and early treatment of mastitis, effectively guiding the selection of antibacterial drugs.

[0007] To achieve the above objectives, the utility model adopts the following technical solutions:

[0008] A concentrated detection device for cow mastitis bacterial liquid, characterized in that it includes a propulsion component, a bacterial liquid concentration processing chamber, and a test strip detection reaction cup. The propulsion component includes a piston handle and a puncture tip provided at the lower end of the piston handle. The bacterial liquid concentration processing chamber is successively provided with a sample loading chamber, a sample recovery chamber, and a reaction tank from the chamber mouth to the chamber bottom. The sample recovery chamber consists of two spaced apart in the middle, and the gap area formed by the middle interval is a concentrated bacterial liquid diversion column. The upper ends of the two sample recovery chambers are respectively covered with bacterial liquid filters, and the upper end of the concentrated bacterial liquid diversion column is covered with a central sealing film. The puncture tip is arranged opposite to the concentrated bacterial liquid diversion column. The test strip detection reaction cup is arranged on the outer wall of the bacterial liquid concentration processing chamber. A card slot and a test strip installed in the card slot are arranged in the test strip detection reaction cup, and the bottom of the test strip detection reaction cup body is communicated with the reaction tank.

[0009] Preferably, the lower end opening of the concentrated bacterial liquid diversion column is communicated with the reaction tank. When the piston handle is pushed downward along the inner wall of the sample loading chamber, the puncture tip pierces the central sealing film, and the concentrated bacterial liquid filtered by the bacterial liquid filter flows into the reaction tank through the concentrated bacterial liquid diversion column, and then contacts the test strip in the bottom of the test strip detection reaction cup body and conducts a detection reaction.

[0010] Preferably, the reaction tank is arranged in a slope, and the slope height gradually decreases from the lower end opening of the concentrated bacterial liquid diversion column to the bottom of the test strip detection reaction cup body; the slope setting is beneficial to the rapid reflux and aggregation of the concentrated bacterial liquid into the reaction tank to realize the immersion test of the test strip.

[0011] Preferably, a plurality of air holes are provided at the upper end of the test strip detection reaction cup body; after piercing, with the continuous advancement of the piston, due to the setting of the air holes, the atmospheric resistance can be reduced, which is beneficial to the rapid recovery of the concentrated cow bacterial liquid sample.

[0012] Preferably, bearing frames are arranged at both ends of the sample recovery chamber close to the inner wall of the bacterial liquid concentration processing chamber, and the setting of the bearing frames is beneficial to supporting the bacterial liquid filter.

[0013] Preferably, the piston handle is divided into a piston propulsion handle and a piston compression section. The lower end of the piston compression section is in an inverted triangle shape, and the inverted triangle structure can reduce the propulsion resistance and accelerate the concentration process; the puncture tip is located at the bottom of the inverted triangle.

[0014] Preferably, the diameter size of the piston compression section is the same as the inner diameter of the sample loading chamber.

[0015] Preferably, the test strips are Gram-positive bacteria test strips and Gram-negative bacteria test strips.

[0016] Preferably, the Gram-positive bacteria test strip is a Staphylococcus aureus test strip or a Streptococcus test strip, and the Gram-negative bacteria test strip is a Klebsiella pneumoniae test strip, an Escherichia coli test strip, a Salmonella test strip, a Pasteurella test strip, and a Brucella test strip.

[0017] Preferably, the bacterial liquid filter is a filter membrane with a pore size of 0.20 - 0.24 μm, which can effectively retain bacteria in the milk sample and achieve the enrichment of bacteria.

[0018] After the milk sample is poured into the sample loading bin of the bacterial liquid concentration treatment bin, with the advancement of the piston handle, through the filtering action of the bacterial liquid filter, the milk sample is filtered into the sample recovery bin, and the concentrated bacterial liquid is retained above the bacterial liquid filter. With the further advancement of the piston handle, the puncture tip pierces the central sealing film, and the concentrated bacterial liquid enters the reaction tank through the concentrated bacterial liquid diversion column, and then contacts the test strip at the bottom of the test strip detection reaction cup body for a detection reaction. Observe the detection result of the test strip for 10 - 15 minutes.

[0019] Preferably, the sample loading bin can accommodate 100 - 120 ml of milk sample. After concentration, 0.8 ml - 1.5 ml of concentrated bacterial liquid can be obtained, achieving a hundred-fold concentration within 5 minutes, and meeting the test sensitivity requirements to the greatest extent through detection.

[0020] Compared with the prior art, the present utility model has the following beneficial effects:

[0021] (1) Provide a device for concentrating and detecting bacterial liquid of cow mastitis. Through the structure and connection setting relationship of the pushing and piercing assembly, the bacterial liquid concentration treatment bin, and the test strip detection reaction cup, it integrates sample concentration and detection. The result can be obtained within 20 minutes, greatly shortening the diagnosis time and saving manpower and material resources;

[0022] (2) It can simultaneously detect Gram-positive bacteria and Gram-negative bacteria, maximizing the detection to ensure that all bacteria to be detected are detected without omission;

[0023] (3) It can detect a variety of bacteria, and the detection results of each bacterial infection are presented independently, which can provide targeted guidance for treatment, avoid drug abuse, and promote the healthy development of the aquaculture industry. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 is a schematic structural diagram of the device of the present utility model after detection and use.

[0025] Figure 2 is a cross-sectional view of the device structure of the present utility model.

[0026] Figure 3 is a schematic diagram of the exhaust hole setting of the device of the present utility model.

[0027] Figure 4 Schematic diagram of the diversion groove setting of the device of the present utility model.

[0028] Figure 5 Schematic diagram of the structure of the concentration and detection process of the device of the present utility model.

[0029] Figure 6 Schematic diagram of the detection result of the test strip of the device of the present utility model.

[0030] In the figure, the attached drawing reference signs are: 1. Pushing and puncturing assembly, 1-1. Piston handle, 1-11. Piston pushing handle, 1-12. Piston compression section, 1-2. Puncturing tip, 2. Bacterial liquid concentration treatment chamber, 2-1. Sample loading chamber, 2-2. Bacterial liquid filter, 2-3. Central sealing film, 2-4. Sample recovery chamber, 2-5. Concentrated bacterial liquid diversion column, 2-6. Reaction tank, 2-7. Carrier, 2-8. Ventilation hole, 3. Test strip detection reaction cup, 3-1. Card slot, 3-2. Test strip, 3-3. Ventilation hole, 14. Staphylococcus aureus test strip, 15. Streptococcus test strip, 16. Klebsiella pneumoniae test strip, 17. Escherichia coli test strip, 18. Salmonella test strip, 19. Pasteurella test strip, 20. Brucella test strip, 4. Concentrated bacterial liquid. Detailed implementation manners

[0031] The following further describes the present utility model in conjunction with the attached drawings and specific implementation manners. It should be understood that the described preferred embodiments are only used to illustrate and explain the present utility model, and are not used to limit the present utility model.

[0032] Embodiment 1

[0033] A device for concentrating and detecting bacterial liquid of cow mastitis, as Figure 1 shown, the device includes a pushing and puncturing assembly 1, a bacterial liquid concentration treatment chamber 2, and a test strip detection reaction cup 3.

[0034] As Figure 2 , 3 , 4 shown, the pushing and puncturing assembly 1 includes a piston handle 1-1 and a puncturing tip 1-2. The piston handle 1-1 is further subdivided into a piston pushing handle 1-11 and a piston compression section 1-12. The lower end of the piston compression section 1-12 is in an inverted triangle shape, and the inverted triangle structure can reduce the pushing resistance during the pushing process and accelerate the concentration process; the puncturing tip 1-2 is located at the bottom of the inverted triangle at the lower end of the piston compression section 1-12.

[0035] The bacterial liquid concentration treatment chamber 2 is sequentially provided with a sample loading chamber 2-1, a sample recovery chamber 2-4, and a reaction tank 2-6 from the chamber opening to the chamber bottom. The sample recovery chamber 2-4 is composed of two left and right sample recovery chambers arranged at intervals in the middle, and the middle interval gap area formed by the two sample recovery chambers is the concentrated bacterial liquid diversion column 2-5.

[0036] At the upper ends of the two sample recovery bins, there are respectively covered with bacterial liquid filters 2-2. The bacterial liquid filter 2-2 is a filter membrane with a pore size of 0.22 um, and the filter membrane has a certain hardness. The upper end of the concentrated bacterial liquid diversion column 2-5 is covered with a central sealing film 2-3. The puncture tip 1-2 is arranged opposite to the central sealing film 2-3. The diameter size of the piston compression section 1-12 is the same as the inner diameter of the sample loading bin 2-1. When the piston handle 1-1 continuously advances downward along the inner side wall of the sample loading bin 2-1, the puncture tip 1-2 can pierce the central sealing film 2-3.

[0037] The concentrated bacterial liquid diversion column 2-5 is sealed with the central sealing film 2-3 at its upper end and is provided with an opening at the lower end. The lower end opening of the concentrated bacterial liquid diversion column 2-5 is communicated with the reaction tank 2-6; the lower ends of the left and right two sample recovery bins 2-4 are closed (i.e., not communicated with the reaction tank 2-6). When the piston handle 1-1 advances downward along the inner side wall of the sample loading bin 2-1, the milk sample added to the sample loading bin 2-1, after being filtered by the bacterial liquid filter 2-2, flows into the two sample recovery bins 2-4; as the piston handle 1-1 further advances, the puncture tip 1-2 pierces the central sealing film 2-3. Due to the lower end of the piston compression section 1-12 being in an inverted triangle shape and the filter membrane of the bacterial liquid filter 2-2 having a certain hardness, the piston compression section 1-12 presses up the bacterial liquid filter 2-2 covering the upper ends of the two sample recovery bins 2-4, so that the concentrated bacterial liquid 4 gathers at the punctured central sealing film 2-3 and further quickly flows into the reaction tank 2-6 along the concentrated bacterial liquid diversion column 2-5.

[0038] As Figure 4 shown, the test strip detection reaction cup 3 is arranged on the outer wall of the bacterial liquid concentration treatment bin 2. In the test strip detection reaction cup 3, there are provided a card slot 3-1 and a test strip 3-2 installed in the card slot, and it is communicated with the reaction tank 2-6 through the bottom of the test strip detection reaction cup 3.

[0039] In this embodiment, the reaction tank 2-6 is arranged in a slope shape, and the slope height gradually decreases from the lower end opening of the concentrated bacterial liquid diversion column 2-5 to the bottom of the test strip detection reaction cup 3. The slope setting is beneficial for the concentrated bacterial liquid to quickly flow back and gather in the reaction tank, and then contact the test strip 3-2 at the bottom of the test strip detection reaction cup 3 and carry out a detection reaction.

[0040] In this embodiment, at both ends of the two sample recovery bins 2-4 close to the inner side wall of the bacterial liquid concentration treatment bin 2, there is provided a carrier 2-7 respectively. One end of the carrier 2-7 supports on the bacterial liquid filter 2-2, and one end supports on the bottom end of the sample recovery bin 2-4. The width of the carrier 2-7 gradually becomes smaller from the end of the bacterial liquid filter 2-2 to the bottom end of the sample recovery bin 2-4. The setting of the carrier is beneficial for supporting the bacterial liquid filter.

[0041] In this embodiment (asFigure 2 As shown, the right sample recovery bin 2-4 (i.e., the sample recovery bin closer to the test strip detection reaction cup 3), and there is a ventilation hole 2-8 at the upper end of the sample recovery bin wall to facilitate the rapid concentration of the milk sample before the central sealing film 2-3 is punctured.

[0042] In this embodiment, there are seven pathogen test strips in the test strip detection reaction cup 3, namely: Staphylococcus aureus test strip 14, Streptococcus test strip 15, Klebsiella pneumoniae test strip 16, Escherichia coli test strip 17, Salmonella test strip 18, Pasteurella test strip 19 and Brucella test strip 20. The above seven test strips basically cover all the pathogenic bacteria causing mastitis and have good recognition for chronic mastitis and occult mastitis. The test results can reflect which specific bacteria are infected and can effectively guide the treatment and medication.

[0043] In this embodiment, there are three ventilation holes 3-3 provided at intervals at the upper end of the test strip detection reaction cup 3, as Figure 3 shown. After piercing, as the piston continues to advance, due to the setting of the ventilation holes 3-3, the atmospheric resistance can be reduced, which is beneficial to the rapid recovery of the concentrated milk bacterial liquid sample. In some preferred embodiments, the number of ventilation holes 3-3 can be 1-8.

[0044] The device concentration and detection method is as Figure 5 shown:

[0045] Place the test strip 3-2 in the card slot 3-1, and the card slot 3-1 is embedded in the test strip detection reaction cup 3. Remove the piston handle 1-1, add 120 ml of milk sample to the sample loading bin 2-1, then install the piston handle 1-1 and slowly push it to the bottom, which can effectively intercept the bacteria in the milk sample, achieve the concentration and enrichment of the bacteria, and complete the concentration and diversion of the bacterial liquid. When the concentrated bacterial liquid is introduced into the reaction tank 2-6, the test strip starts the chromatography reaction. Start timing, and by observing the display result of the test strip window of the cup body (as Figure 6 shown), the detection of mastitis can be completed. Read the result after 10-15 minutes, and the result is invalid after 20 minutes.

[0046] As Figure 6 shown, through the detection result observation schematic diagram of the test strip of this device, the specific interpretation result criteria are as shown in the following table:

[0047]

[0048]

[0049] The obtained results are rapid, accurate, easy to interpret, have good stability, and have strong practical application value.

[0050] Example 2

[0051] A concentrated detection device for cow mastitis bacterial liquid. Different from Embodiment 1, a sealing ring is provided on the piston compression section 1-12 of the piston handle 1-1, making the connection with the inner wall of the sample loading chamber 2-1 more sealed. When the piston handle 1-1 is pushed downward along the inner wall of the sample loading chamber 2-1, it is more conducive to compressing the original milk sample in the sample loading chamber 2-1 and obtaining the concentrated bacterial liquid 4 after filtering through the bacterial liquid filter 2-2.

[0052] Embodiment 3

[0053] A concentrated detection device for cow mastitis bacterial liquid. Different from Embodiment 1, with the increase in the types of bacteria infections to be detected, the test paper detection reaction cups 3 are circumferentially arranged on the outer wall of the bacterial liquid concentration treatment chamber 2 for one week, and the number of inner card slots (3-1) in the test paper detection reaction cups 3 and the test paper strips (3-2) installed in the card slots is increased, so as to achieve the purpose of screening and detecting more bacteria causing cow mastitis.

Claims

1. A concentrated detection device for cow mastitis bacterial liquid, characterized in that: It includes a propulsion puncturing assembly (1), a bacterial liquid concentration treatment chamber (2), and a test strip detection reaction cup (3). The propulsion puncturing assembly (1) includes a piston handle (1-1) and a puncturing tip (1-2) provided at the lower end of the piston handle. The bacterial liquid concentration treatment chamber (2) is sequentially provided with a sample loading chamber (2-1), a sample recovery chamber (2-4), and a reaction tank (2-6) from the chamber opening to the chamber bottom. The sample recovery chamber (2-4) consists of two parts spaced in the middle, and the gap area formed by the middle space is a concentrated liquid diversion column (2-5). The upper ends of the two sample recovery chambers (2-4) are respectively covered with a bacterial liquid filter (2-2), and the upper end of the concentrated liquid diversion column (2-5) is covered with a central sealing film (2-3). The puncturing tip (1-2) is disposed opposite to the central sealing film (2-3). The test strip detection reaction cup (3) is provided on the outer wall of the bacterial liquid concentration treatment chamber (2), and a card slot (3-1) and a test strip (3-2) installed in the card slot are provided in the test strip detection reaction cup (3). The bottom of the test strip detection reaction cup (3) is communicated with the reaction tank (2-6).

2. The milk cow mastitis bacterial liquid concentration detection device according to claim 1, wherein: The lower end opening of the concentrated liquid diversion column (2-5) is communicated with the reaction tank (2-6). When the piston handle (1-1) is pushed downward along the inner wall of the sample loading chamber (2-1), the puncturing tip (1-2) punctures the central sealing film (2-3), and the concentrated bacterial liquid (4) filtered by the bacterial liquid filter (2-2) flows into the reaction tank (2-6) through the concentrated liquid diversion column (2-5), and then contacts the test strip (3-2) at the bottom of the test strip detection reaction cup (3) and conducts a detection reaction.

3. The milk cow mastitis bacterial liquid concentration detection device according to claim 2, wherein: The reaction tank (2-6) is arranged in a slope, and the slope height gradually decreases from the lower end opening of the concentrated liquid diversion column (2-5) to the bottom of the test strip detection reaction cup (3).

4. A milk cow mastitis bacterial liquid concentration detection device according to claim 1, characterized in that: A number of air holes (3-3) are provided at the upper end of the test strip detection reaction cup (3).

5. The concentrated detection device for cow mastitis bacterial liquid according to claim 1, characterized in that, Carrying racks (2-7) are respectively arranged at both ends of the sample recovery chamber (2-4) close to the inner wall of the bacterial liquid concentration treatment chamber (2).

6. The milk cow mastitis bacterial liquid concentration detection device according to claim 1, characterized in that The piston handle (1-1) is divided into a piston propulsion handle (1-11) and a piston compression section (1-12). The lower end of the piston compression section (1-12) is in an inverted triangle shape, and the puncturing tip (1-2) is located at the bottom of the inverted triangle.

7. The milk cow mastitis bacterial liquid concentration detection device according to claim 6, characterized in that, The diameter size of the piston compression section (1-12) is the same as the inner diameter of the sample loading chamber (2-1).

8. The concentrated detection device for cow mastitis bacterial liquid according to claim 1, characterized in that, The test strip (3-2) is a Gram-positive bacteria test strip and a Gram-negative bacteria test strip.

9. The milk cow mastitis bacterial liquid concentration detection device according to claim 8, characterized in that, The Gram-positive bacteria test strip is a Staphylococcus aureus test strip (14) and a Streptococcus test strip (15), and the Gram-negative bacteria test strip is a Klebsiella pneumoniae test strip (16), an Escherichia coli test strip (17), a Salmonella test strip (18), a Pasteurella test strip (19), and a Brucella test strip (20).

10. The milk cow mastitis bacterial liquid concentration detection device according to claim 1, characterized in that, The bacterial liquid filter (2-2) is a filter membrane with a pore diameter of 0.20-0.24 um.

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