Centrifugal-free cell culture bottle

Abstract

The utility model discloses a centrifugation-free cell culture bottle which comprises a hollow cavity, and a filter component is arranged on the inner wall of the cavity; a gap part is arranged between the filtering assembly and the cavity; a height difference exists between the inner walls of the cavity on the two sides of the filtering assembly, and when liquid is added into the cell culture bottle or taken out of the cell culture bottle, the inner wall close to the opening side of the cavity is lower than the inner wall away from the opening side. The utility model provides the centrifugation-free cell culture bottle which is simple in design, convenient and fast to operate and capable of effectively avoiding centrifugal operation and reducing the risk of cell pollution, and the efficiency and the safety of a cell culture experiment are effectively improved due to the design.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell culture, and relates to a cell culture flask without centrifugation. Background Art

[0002] Cell culture is a method of enabling cells to survive, grow, reproduce and maintain their main structures and functions in vitro. Cell culture is an important and commonly used technique in cell biology research methods. Through cell culture, a large number of cells can be obtained, and cell signal transduction, cell anabolism, cell growth and proliferation, etc. can be studied. During cell culture, when the cell density reaches 80%-90%, cell passage operation needs to be carried out on the cells. For adherent cells, the specific process is as follows: remove the original culture medium, add cell digestive juice to digest the cells. After resuspending the cells, transfer them to a centrifuge tube for centrifugation operation, precipitate the cells and discard the supernatant. Then add fresh culture medium and culture the cells in sub-flasks to achieve the purpose of cell passage and scale-up culture; for suspension cells, the specific process is as follows: transfer the original cell suspension to a centrifuge tube for centrifugation operation, precipitate the cells and discard the supernatant. Then add fresh culture medium and culture the cells in sub-flasks to passage and scale-up culture the cells. This process is not only cumbersome, but also increases the contamination of cells by exogenous microorganisms such as mycoplasma, bacteria, and fungi in the environment.

[0003] The Chinese utility model patent with the publication number CN217757521 U discloses a suspension cell culture flask. The suspension cell culture flask provided by this utility model uses a filter screen as a partition board to divide the inner cavity of the culture flask into two independent upper and lower cavities, and the upper and lower cavities each use independent bottle mouths for liquid replacement operations; the mesh holes of the filter screen allow liquid and / or gas molecules to pass through, but block the passage of cells, realizing cell isolation and dispersion, making the cells dispersed, which is beneficial to the rapid growth of cells, and at the same time reducing the pollution caused by liquid replacement. The structure of this device is relatively complex. The Chinese utility model patent with the publication number CN214572024 U discloses a cell culture flask with a filtering device, including a culture flask, a protective rubber pad, a bottle mouth, and an automatic filter screen mechanism. A fixed block is fixedly installed on one side of the outer part of the culture flask; a magnet block is clamped and fixed on the fixed block. When pouring the culture flask, the magnet block is attracted to the metal strip, so that the automatic filter screen mechanism is placed horizontally inside the culture flask to prevent solid substances from being poured out together with the liquid when changing the liquid during cell culture. The Chinese utility model patent with the publication number CN214032460 U discloses a cell culture flask convenient for liquid replacement. In this utility model, when supplementing the culture solution, the culture solution enters the main body of the culture flask above the partition through the liquid inlet pipe, and then by rotating the screw rod, the lifting piston and the sealing ring are driven to slide downward along the inner cavity of the main body of the culture flask. At this time, the culture solution enters the main body of the culture flask below the partition under the action of pressure, and cell culture is carried out above the filter screen; when it is necessary to change the liquid, the screw rod is rotated in the reverse direction, driving the lifting piston and the sealing ring to slide upward along the inner cavity of the main body of the culture flask. At this time, the waste liquid to be replaced is discharged and collected through the liquid outlet pipe by the drain pipe, while the cells remain on the filter screen to prevent waste. This utility model uses the lifting piston to realize the process of the inlet and outlet of the culture solution, without pouring the culture flask, further preventing pollution, and the setting of the filter screen can effectively prevent cell waste and is convenient for liquid replacement. The structures of existing cell culture flasks are all relatively complex, with high manufacturing costs and inconvenient operations, and the liquid replacement process in the cell culture flask cannot be achieved quickly. Summary of the Utility Model

[0004] Aiming at the problems existing in the prior art, the present utility model provides a cell culture flask without centrifugation, so as to solve the technical problems that the existing cell culture flask has a complex structure, high manufacturing cost, inconvenient operation, and cannot quickly realize the liquid replacement process in the cell culture flask.

[0005] The present utility model is realized through the following technical solutions:

[0006] A cell culture flask without centrifugation includes a hollow cavity, and a filtering component is provided on the inner wall of the cavity;

[0007] A gap part is provided between the filtering component and the cavity;

[0008] The inner walls of the cavities on both sides of the filtering component have a height difference. When adding or removing liquid from the cell culture flask, the inner wall closer to the opening of the cavity is lower than the inner wall farther away from the opening.

[0009] Preferably, the cavity includes a first bottle body and a second bottle body which are connected. In the direction perpendicular to the axis of the cavity, the cross-sectional height of the first bottle body is greater than that of the second bottle body.

[0010] Preferably, the cross-section of the inner wall of the first bottle body along the axial direction of the cavity is trapezoidal, rectangular or arc-shaped.

[0011] Preferably, when the cross-section of the inner wall of the first bottle body along the axial direction of the cavity is rectangular, a cushion block with an inclined surface is arranged on the inner wall of the first bottle body.

[0012] Preferably, the cross-section of the inner wall of the second bottle body along the axial direction of the cavity is trapezoidal, rectangular or arc-shaped.

[0013] Preferably, the first bottle body and the second bottle body are integrally arranged.

[0014] Preferably, the aperture of the filtering component is less than 5 μm.

[0015] Preferably, the height of the gap part is 1 - 2 cm.

[0016] Preferably, the height difference is 2 - 4 cm.

[0017] Preferably, the outer wall of the cavity is rectangular.

[0018] Compared with the prior art, the utility model has the following beneficial technical effects:

[0019] The utility model discloses a centrifugation-free cell culture bottle, wherein the main body of the cell culture bottle is a hollow cavity, and the interior thereof is designed by a simple structure, including a filter assembly and a gap portion, so that the culture fluid can be replaced without centrifugation. When the culture fluid needs to be replaced, the user only needs to flip the cell culture bottle so that the liquid flows to a position close to the opening through the filter assembly. At the same time, the filter assembly can effectively intercept cells to prevent them from flowing out with the culture fluid. This operation method is simple and easy, and does not require complicated operation equipment or steps. The setting of the gap portion makes it easy to add new culture fluid on the one hand, and on the other hand, it is easy for cells to be distributed to the entire culture bottle, which is convenient for their growth. Through the centrifugation-free design, the cell contamination problem that may be caused by traditional centrifugation operation is avoided. The setting of the filter assembly enables the cells to be effectively retained in the culture bottle when the culture fluid is replaced, thereby reducing the risk of cell contamination. The height difference design of the inner wall of the cavity on both sides of the filter assembly enables the culture fluid to be replaced to be gathered on the lower side, so that it is effectively separated and convenient for the user to take it out. At the same time, after adding new culture fluid, by flipping the cell culture bottle again, the cells can be quickly dispersed in the new culture fluid, thereby realizing efficient replacement of the culture fluid. The centrifugation-free cell culture bottle is suitable for various cell culture experiments, especially for experimental projects that require frequent replacement of culture fluid, and its advantages are more obvious. The utility model provides a centrifugation-free cell culture bottle with simple design, convenient operation, and the ability to effectively avoid centrifugation operation and reduce the risk of cell contamination. The design effectively improves the efficiency and safety of cell culture experiments.

[0020] Further, the cavity includes a first bottle body and a second bottle body connected and arranged, and in a direction perpendicular to the axis of the cavity, the cross-sectional height of the first bottle body is greater than the cross-sectional height of the second bottle body. Since the cross-sectional height of the first bottle body is larger, when the cell culture bottle is turned over, more culture fluid will flow to a position close to the opening, thereby facilitating the removal and addition of liquid. The design of the second bottle body, although the cross-sectional height is smaller, is sufficient to accommodate cells, so that when the culture fluid is replaced, the cells can be stably retained in the second bottle body to avoid loss. The smaller cross-sectional height design of the second bottle body makes it difficult for cells to move due to liquid flow during the turning process, further reducing the possibility of cell loss. The connection design of the first bottle body and the second bottle body makes the entire cell culture bottle more stable during operation and not easy to tip over or slide. By reducing cell loss, the design ensures the stability of the number of cells during the experiment, thereby improving the accuracy and reliability of the experimental results. The efficient culture fluid replacement process also helps to reduce the stress response of cells during the replacement process, further improving the accuracy of the experimental results.

[0021] Furthermore, the cross-section of the inner wall of the first bottle body along the axial direction of the cavity is trapezoidal, rectangular or arc-shaped. Further, the cross-section of the inner wall of the second bottle body along the axial direction of the cavity is trapezoidal, rectangular or arc-shaped. The trapezoidal shape can guide the culture medium to flow more smoothly towards the opening when the bottle is flipped. Especially when the lower base of the trapezoid (i.e., the side closer to the opening) is wider, this effect is more significant. This helps to accelerate the discharge of the culture medium and the addition of new culture medium. The inclined surface design of the trapezoid helps the cells to gather towards the second bottle body during the flipping process, reducing the risk of cell loss with the culture medium. By adjusting the angle and size of the trapezoid, it can meet the requirements of different cell types and experimental conditions, improving the flexibility and adaptability of the experiment. The rectangular cross-section has a simple geometric shape, which is easy to manufacture and clean. This helps to reduce production costs, improve production efficiency, and reduce experimental errors caused by improper cleaning. The rectangular cross-section is easy to standardize and mass-produce, and is suitable for laboratories or production environments that require a large number of cell culture bottles. The arc-shaped cross-section can provide stronger structural strength, making the cell culture bottle more stable and safe when subjected to external impact or vibration. The arc-shaped cross-section can reduce dead corners and residues on the inner wall, reducing the risk of cell contamination caused by improper cleaning.

[0022] Furthermore, when the cross-section of the inner wall of the first bottle body along the axial direction of the cavity is rectangular, a spacer block with an inclined surface is provided on the inner wall of the first bottle body. The spacer block with an inclined surface can change the flow characteristics of the culture medium in the first bottle body. When the culture bottle is flipped, the inclined surface of the spacer block can guide the culture medium to gather more smoothly at the opening of the cavity, thus accelerating the discharge process of the culture medium. The design of the inner wall of the first bottle body with a rectangular cross-section in combination with the inclined surface spacer block makes cleaning and maintenance more convenient. During cleaning, the inclined surface of the spacer block can guide the cleaning liquid to flow, ensuring that the cleaning liquid can cover the entire inner wall surface and reducing the existence of dead corners and residues.

[0023] Furthermore, the first bottle body and the second bottle body are integrally provided. The integral design ensures that there is no connecting gap between the first bottle body and the second bottle body, thus greatly enhancing the structural stability of the entire cell culture bottle. This helps reduce the risk of rupture or leakage caused by external impacts or improper operations, ensuring the safety and reliability of the experiment. The integral design reduces potential connecting gaps that could serve as breeding grounds for bacteria, viruses, or other contaminants. By eliminating these gaps, the integral design significantly reduces the risk of contamination during cell culture, guaranteeing the accuracy and reliability of experimental results. The integral design simplifies the manufacturing process of the cell culture bottle as no additional connecting steps or components are required. This not only reduces production costs but also improves production efficiency, making the manufacturing of cell culture bottles more economical and efficient. The integral design generally has better sealing performance as there is no potential leakage risk at the connection. This helps maintain the stability of the internal environment of the cell culture bottle, ensuring that cells grow and reproduce under constant conditions such as temperature, humidity, and gas concentration.

[0024] Furthermore, the pore size of the filtration component is less than 5 μm. The size of cells is usually greater than 5 μm. Therefore, using a filtration component with a pore size less than 5 μm can effectively prevent cells from being lost with the liquid when changing the culture medium. This is crucial for maintaining the stability of the cell count during the experiment, ensuring the accuracy and reliability of experimental results. Microorganisms such as bacteria, fungi, and viruses are usually smaller than cells. By using a filtration component with a pore size less than 5 μm, the risk of these microorganisms entering the cell culture environment through the filtration component can be further reduced, thereby decreasing the likelihood of microbial contamination. This is crucial for maintaining the sterility of the cell culture environment and helps protect cells from contamination and damage. The filtration component can not only prevent cell loss and microbial contamination but also remove impurities and particles in the culture medium. These impurities and particles may affect cell growth and reproduction and even have a toxic effect on cells. By filtration, the quality of the culture medium can be improved, providing a purer and more suitable growth environment for cells. Since the filtration component can effectively prevent cell loss and microbial contamination and improve the quality of the culture medium, using such a filtration component can enhance the repeatability of the experiment. Between different experimental batches, factors such as cell count, culture medium quality, and environmental conditions can remain relatively stable, thus reducing the fluctuations and uncertainties of experimental results. Using a filtration component with a pore size less than 5 μm can simplify the operation process during cell culture. When changing the culture medium, users do not need to worry about cell loss or microbial contamination. They only need to connect the filtration component to the cell culture bottle and then pour out the old culture medium and add the new one. This simplified operation process reduces the complexity and difficulty of experimental operations and improves experimental efficiency.

[0025] Furthermore, the height of the gap portion is 1 to 2 cm, and the appropriate height of the gap portion enables the user to more clearly observe the growth of cells in the first bottle body and the second bottle body. This is very important for monitoring the growth status of cells, adjusting experimental conditions, and timely discovering problems. The design of the gap portion can increase the overall structural stability of the cell culture bottle. When subjected to external force, the gap portion can play a certain buffering role and reduce damage to the entire culture bottle. The appropriate height of the gap portion makes the manufacturing process of the cell culture bottle simpler, and is also easy to clean and maintain. The design of the gap portion can reduce cleaning dead corners and reduce the risk of contamination.

[0026] Furthermore, the height difference is 2 to 4 cm, and the height difference of 2 to 4 cm can ensure that the culture fluid can flow smoothly from the first bottle body to the second bottle body when the cell culture bottle is flipped or tilted. This flow helps to effectively replace the culture fluid while reducing the loss of cells during the transfer process. The height difference of 2 to 4 cm allows the user to more easily control the flow of liquid when flipping or tilting the cell culture bottle. This convenience helps to improve the efficiency and accuracy of experimental operations.

[0027] Furthermore, the outer wall of the cavity is rectangular, and the rectangular outer wall shape makes the cell culture bottle more stable when placed, and is not easy to tip over or roll. This stability is particularly important for cell culture experiments that need to be stationary for a long time, which can ensure that the cells grow in a stable environment. The rectangular outer wall shape allows the cell culture bottles to be neatly stacked together, which is convenient for storage and transportation in the laboratory or warehouse. This design can save space and improve storage efficiency. The rectangular outer wall shape usually meets the size requirements of standard experimental equipment, such as shakers, incubators, etc. This allows the cell culture bottles to be easily placed in these devices for culture without the need for additional adapters or adjustments. The rectangular outer wall shape has no complex curves or edges, making the cleaning and disinfection process simpler and more efficient. This helps to reduce the risk of contamination and ensure the accuracy of the experimental results. The rectangular outer wall shape can provide better structural strength, making the cell culture bottle more durable when subjected to external forces. This is particularly important for experiments that require frequent movement or operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the utility model and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying creative work.

[0029] Figure 1 A side view of a centrifugation-free cell culture bottle in one embodiment of the utility model;

[0030] Figure 2 Top view of a cell culture flask without centrifugation in an embodiment of the present utility model;

[0031] Figure 3 View of another perspective of a cell culture flask without centrifugation in an embodiment of the present utility model;

[0032] Figure 4 Schematic diagram of cell culture using a cell culture flask without centrifugation in an embodiment of the present utility model;

[0033] Figure 5 Schematic diagram of the position change when changing the culture medium using a cell culture flask without centrifugation in an embodiment of the present utility model;

[0034] Figure 6 Schematic diagram of the setting of the spacer block in a cell culture flask without centrifugation in an embodiment of the present utility model;

[0035] Figure 7 Cross-sectional view of a cell culture flask without centrifugation in an embodiment of the present utility model;

[0036] Figure 8 Cross-sectional view of a cell culture flask without centrifugation in another embodiment of the present utility model.

[0037] Wherein: 1, cavity; 2, filtration assembly; 3, clearance part; 11, first bottle body; 12, second bottle body; 13, spacer block; 4, cells; 5, culture medium; 6, fresh culture medium. Detailed implementation manners

[0038] To make the objectives, technical solutions and advantages of the embodiments of the present utility model clearer, the technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are some but not all of the embodiments of the present utility model. Generally, the components of the embodiments of the present utility model described and illustrated herein can be arranged and designed in various different configurations.

[0039] Therefore, the following detailed description of the embodiments of the present utility model provided in the drawings is not intended to limit the scope of the present utility model to be protected, but merely represents the selected embodiments of the present utility model. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts shall fall within the protection scope of the present utility model.

[0040] It should be noted that similar reference numerals and letters indicate similar items in the following drawings. Therefore, once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings.

[0041] In the description of the embodiments of the present invention, it should be noted that if terms such as "upper", "lower", "horizontal", "inner", etc. are used to indicate the orientation or positional relationship, it is based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship in which the product of the present invention is usually placed during use. This is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation to the present invention. In addition, terms such as "first", "second", etc. are only used for distinguishing descriptions and cannot be understood as indicating or implying relative importance.

[0042] In addition, if the term "horizontal" appears, it does not mean that the component is required to be absolutely horizontal, but it can be slightly inclined. For example, "horizontal" only means that its direction is more horizontal relative to "vertical", and does not mean that the structure must be completely horizontal, but it can be slightly inclined.

[0043] In the description of the embodiments of the present invention, it should also be noted that unless otherwise clearly specified and limited, if terms such as "set", "installed", "connected", "connected" are used, they should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0044] The present invention will be further described in detail below with reference to the drawings:

[0045] Embodiment 1

[0046] The present invention discloses a cell culture bottle without centrifugation, which includes a hollow cavity 1, and a filtering component 2 is provided on the inner wall of the cavity 1; a gap part 3 is provided between the filtering component 2 and the cavity 1, and the gap part 3 allows fluid to pass through; that is, a part of the filtering component 2 is fixedly connected to the inner wall of the cavity 1, and a part is provided with a gap part 3 between it and the cavity 1. There is a height difference between the inner walls of the cavities on both sides of the filtering component 2. When adding or removing liquid from the cell culture bottle, the inner wall near the opening of the cavity 1 is lower than the inner wall far from the opening.

[0047] Such as Figures 1 to 3As shown, preferably, the cavity 1 includes a first bottle body 12 and a second bottle body 11 which are connected. In the direction perpendicular to the axis of the cavity 1, the cross-sectional height of the first bottle body 12 is greater than the cross-sectional height of the second bottle body 11.

[0048] As Figure 1 shown, the cross-section of the inner wall of the first bottle body 12 along the axial direction of the cavity 1 is rectangular, and the cross-section of the inner wall of the second bottle body 11 along the axial direction of the cavity 1 is also rectangular. A stepped structure is formed due to the height difference between the inner walls of the first bottle body 12 and the second bottle body 11. The schematic diagram of the setting when bacteria grow in this cell culture bottle is shown in Figure 4 shown. The cells 4 and the culture medium 5 are located on the side where the filtering component 2 is not provided. The first bottle body 12 and the second bottle body 11 are integrally provided.

[0049] In addition, for the cell culture bottles in different embodiments, when changing the culture medium, the schematic diagram of the position of the cell culture bottle is shown in Figure 5 , specifically: the cell culture bottle is placed upright, and the cells 4, which can be adherent cells, are located at the bottom of the cell culture bottle. Remove the original culture medium, add cell digestive fluid to digest the cells ( Figure 5 A in). After resuspending the cells, turn over the cell culture bottle ( Figure 5 B in), so that the resuspended cells are located on the upper layer of the stepped structure, and the filter membrane filters the digestive fluid into the lower layer of the stepped structure ( Figure 5 C in). Remove the digestive fluid, add fresh culture medium 6 to the upper layer of the stepped structure ( Figure 5 D in), mix evenly and then turn the cells back to the original position ( Figure 5 E in), and then subsequent cell passage operations can be carried out.

[0050] For suspension cells, except for the digestion process, other steps are similar to those of adherent cells, including: culturing the suspension cells upright, turning over the cell culture bottle, making the suspension cells located on the upper layer of the stepped structure, filtering the original cell culture medium into the lower layer of the stepped structure by the filter membrane, removing the original cell culture medium, adding fresh culture medium to the upper layer of the stepped structure, mixing evenly and then turning the cells back to the original position, and then subsequent cell passage operations can be carried out.

[0051] The pore size of the filtering component 2 is less than 5 μm, which can effectively intercept cells. The height of the gap part 3 is 1 - 2 cm. On the one hand, it is convenient for cells to grow evenly between the first bottle body 12 and the second bottle body 11. At the same time, it is convenient to directly add culture medium to the cells after filtering the culture medium.

[0052] The height difference is 2 - 4 cm. The outer wall of the cavity 1 is rectangular.

[0053] Example 2

[0054] In addition, as Figure 6As shown, preferably, when the cross-section of the inner wall of the first bottle body 12 along the axial direction of the cavity 1 is rectangular, a cushion block 13 with an inclined surface is provided on the inner wall of the first bottle body 12. The provision of the cushion block 13 facilitates the aggregation and replacement of the culture solution.

[0055] Embodiment 3

[0056] Preferably, as Figure 7 shown, the cross-section of the inner wall of the first bottle body 12 along the axial direction of the cavity 1 is trapezoidal, and the cross-section of the inner wall of the second bottle body 11 along the axial direction of the cavity 1 is trapezoidal.

[0057] Preferably, on one side of the filter assembly 2 provided inside the first bottle body 12, a cushion block 13 can also be provided to facilitate the extraction of the culture solution.

[0058] Embodiment 4

[0059] Preferably, as Figure 8 shown, the cross-section of the inner wall of the first bottle body 12 along the axial direction of the cavity 1 is arc-shaped, and the cross-section of the inner wall of the second bottle body 11 along the axial direction of the cavity 1 is arc-shaped. Of course, preferably, in this embodiment, on one side of the filter assembly 2 provided on the inner wall of the second bottle body 11, a cushion block 13 can also be provided to facilitate the extraction of the culture solution.

[0060] The above are only the preferred embodiments of the present utility model and are not used to limit the present utility model. For those skilled in the art, the present utility model can have various changes and modifications. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model shall be included within the protection scope of the present utility model.

Claims

1. A cell culture flask without centrifugation, characterized in that, It includes a hollow cavity (1), and a filtering component (2) is provided on the inner wall of the cavity (1); A gap portion (3) is provided between the filtering component (2) and the cavity (1); There is a height difference between the inner walls of the cavity on both sides of the filtering component (2). When adding or removing liquid from the cell culture bottle, the inner wall closer to the opening of the cavity (1) is lower than the inner wall farther from the opening.

2. The centrifugation-free cell culture flask according to claim 1, characterized in that The cavity (1) includes a first bottle body (12) and a second bottle body (11) connected. In the direction perpendicular to the axis of the cavity (1), the cross-sectional height of the first bottle body (12) is greater than the cross-sectional height of the second bottle body (11).

3. The centrifugation-free cell culture flask according to claim 2, wherein, The cross-section of the inner wall of the first bottle body (12) along the axis of the cavity (1) is trapezoidal, rectangular or arc-shaped.

4. A cell culture flask without centrifugation according to claim 3, wherein, When the cross-section of the inner wall of the first bottle body (12) along the axis of the cavity (1) is rectangular, a cushion block (13) with an inclined surface is provided on the inner wall of the first bottle body (12).

5. The cell culture flask without centrifugation according to claim 2, wherein, The cross-section of the inner wall of the second bottle body (11) along the axis of the cavity (1) is trapezoidal, rectangular or arc-shaped.

6. The cell culture flask without centrifugation according to claim 2, wherein The first bottle body (12) and the second bottle body (11) are integrally provided.

7. The cell culture flask without centrifugation according to claim 1, characterized in that, The pore diameter of the filtering component (2) is less than 5 μm.

8. A cell culture flask without centrifugation according to claim 1, characterized in that, The height of the gap portion (3) is 1 - 2 cm.

9. A cell culture flask without centrifugation according to claim 1, characterized in that, The height difference is 2 - 4 cm.

10. The cell culture flask without centrifugation according to claim 1, characterized in that, The outer wall of the cavity (1) is rectangular.

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