Fluorescence excitation device and sample processing equipment

Abstract

The utility model relates to a fluorescence excitation device and sample processing equipment. The fluorescence excitation device comprises a base; the excitation light source module is fixedly arranged on the base; the reflection module is rotatably arranged on the base, and the reflection module is provided with a reflection channel; the fluorescent module is rotatably arranged on the base, and a light through hole is formed in the fluorescent module; when the reflection module rotates to correspond to the excitation light source module and the fluorescent module rotates to correspond to the reflection channel, the wavelength of the fluorescent module corresponds to the wavelength of the excitation light source module, and excitation light emitted by the excitation light source module is reflected by the reflection module, then is emitted out through the reflection channel and enters the fluorescent module through the light through hole. The excitation light source module and the fluorescence module are independently arranged, and free switching between the excitation light source module and the fluorescence module can be realized according to the requirement of a fluorescence experiment, so that the experiment requirements of different fluorescence illumination are met.

Description

Technical Field

[0001] This application relates to the field of fluorescence technology. Specifically, it relates to a fluorescence excitation device and a sample processing device. Background Art

[0002] Fluorescence is based on the phenomenon that after a substance absorbs energy, through the excitation and transition of electrons, it emits light of a specific wavelength. It can be understood that when light of a specific wavelength irradiates a substance and excites light with a longer wavelength than the irradiating light, this phenomenon is called fluorescence. Among them, the irradiating light is called the excitation light, and the excited light is called fluorescence. Fluorescence technology utilizes the characteristic of the fluorescence phenomenon that a substance emits visible light after being excited by the excitation light, and it has been widely used in fields such as gene detection and biochemical research.

[0003] A fluorescence excitation device usually needs to be designed as an optical system for guiding the light emitted by the excitation source to irradiate the fluorescent dye and collecting the emitted fluorescent light. This optical system generally includes optical elements such as lenses, filters, and mirrors to ensure the effective transmission and collection of excitation and fluorescence.

[0004] In the existing fluorescence excitation devices, when there are many fluorescence modules, the volume of the fluorescence excitation device becomes too large. In addition, the switching of the excitation light source in the fluorescence excitation device causes the light beam of the excitation light source to rotate accordingly, resulting in chaotic circuits. Moreover, the frequent switching of the fluorescence module and the excitation light source makes the addition or replacement of the fluorescence module and the excitation light source more cumbersome, ultimately affecting the working efficiency of the fluorescence excitation device. Summary of the Utility Model

[0005] The purpose of this application is to provide a fluorescence excitation device and a sample processing device, in which the excitation light source module and the fluorescence module are independently arranged, and according to the needs of fluorescence experiments, the free switching of the excitation light source module and the fluorescence module can be realized to meet the experimental requirements of different fluorescence illuminations.

[0006] The embodiments of this application are implemented as follows:

[0007] In a first aspect, this application provides a fluorescence excitation device, including: a base; an excitation light source module fixedly arranged on the base; a reflection module rotatably arranged on the base, the reflection module having a reflection channel; a fluorescence module rotatably arranged on the base, the fluorescence module being provided with a light through-hole; when the reflection module rotates to correspond to the excitation light source module and the fluorescence module rotates to correspond to the reflection channel, the wavelength of the fluorescence module corresponds to the wavelength of the excitation light source module, and the excitation light emitted by the excitation light source module is reflected by the reflection module and then emitted from the reflection channel and enters the fluorescence module through the light through-hole.

[0008] In the above technical solution, the excitation light source module and the fluorescence module can be controlled separately, which is more convenient for independent installation and debugging. The excitation light source module is fixedly arranged, and the rotation of the reflection module is used to replace the movement of the excitation light source, so that the wire harness of the excitation light source module will not be bent as the light source moves, which is more convenient for fixing. When the reflection module rotates to correspond to the excitation light source module, by rotating the fluorescence module, the fluorescence module with a wavelength corresponding to that of the excitation light source module is rotated to make the light through-hole correspond to the reflection channel of the reflection module. At this time, the excitation light emitted by the excitation light source module is projected onto the reflection module, reflected by the reflection module and then emitted through the reflection, and enters the fluorescence module through the light through-hole to achieve fluorescence illumination. Fluorescence detection and analysis of the sample to be detected can be realized through fluorescence.

[0009] In one embodiment, the excitation light source module includes: at least one excitation light source component, and the excitation light source components are arranged at intervals around the reflection module as the center.

[0010] In the above technical solution, a plurality of excitation light source components can be provided, and each excitation light source component can realize the excitation of an excitation light source with a specific wavelength, so as to meet the different detection requirements of fluorescence experiments.

[0011] In one embodiment, the excitation light source component includes: a light source base, an excitation light source and an excitation sheet; the light source base is fixedly arranged on the base, a light source excitation channel is arranged in the light source base, the excitation light source is arranged at the incident end of the light source excitation channel in the light source base, and the excitation sheet is arranged in the light source base and at the exit end of the light source excitation channel.

[0012] In the above technical solution, the excitation light source component is fixedly arranged, and the rotation of the reflection module is used to replace the movement of the excitation light source component, so that the wire harness of the excitation light source component will not be bent as the light source moves, which is more convenient for fixing.

[0013] In one embodiment, the reflection module includes: a first driving component and a reflection component; the first driving component is used to drive the reflection component to rotate so that the reflection component corresponds to the excitation light source module.

[0014] In the above technical solution, according to the light source with a specific wavelength required for the fluorescence experiment, the first driving component can be used to drive the reflection component to rotate so that the reflection component corresponds to the excitation light source component with a set specific wavelength. The excitation light with a specific wavelength emitted by the excitation light source component is projected onto the reflection component, and then the reflection component reflects the excitation light source. To achieve free switching of excitation light sources with different wavelengths.

[0015] In one embodiment, the reflection component includes: a reflection rotation part and a reflector; the first driving component is connected to the reflection rotation part, the reflector is disposed on the reflection rotation part, and the reflection rotation part is rotatably disposed on the base.

[0016] In the above technical solution, the direction of the excitation light source emitted by the excitation light source component can be adjusted to the reflection channel through the reflection component.

[0017] In one embodiment, the reflection channel penetrates through the reflection rotation part and communicates with the reflector.

[0018] In the above technical solution, the reflection channel serves as the reflection path channel of the excitation light source, and can introduce the excitation light of the excitation light source component into the fluorescence component.

[0019] In one embodiment, the fluorescence excitation device further includes: a first limiting module; the first limiting module is used to limit the reflection rotation part when the reflection rotation part rotates.

[0020] In the above technical solution, the first limiting module can limit the reflection rotation part when the reflection rotation part rotates, preventing the reflection rotation part from rotating.

[0021] In one embodiment, the first limiting module includes: a first mounting base, a first limiting member and an elastic member; the first mounting base is disposed on the base, the first limiting member is rotatably disposed on the first mounting base, one end of the elastic member is connected to the first mounting base, and the other end is connected to the first limiting member; a first groove matching the first limiting member is provided on the reflection rotation part.

[0022] In the above technical solution, the structure of the first limiting module is simple and it is easier to achieve the limiting effect.

[0023] In one embodiment, the fluorescence module includes: a second driving component, a fluorescence rotation part and at least one fluorescence component; the fluorescence components are arranged at intervals in a ring on the fluorescence rotation part, the second driving component is connected to the fluorescence rotation part and is used to drive the fluorescence rotation part to rotate so as to drive the fluorescence components to rotate, so that any one of the fluorescence components corresponds to the reflection channel.

[0024] In the above technical solution, when it is necessary to switch the excitation light source of different wavelengths, the reflection component can be driven to rotate by the first driving component, so that the reflection component rotates to the position where the excitation light source component of the required specific wavelength is located. At the same time, the fluorescence rotating part is driven to rotate by the second driving component, so that the fluorescence component of the same specific wavelength as the excitation light source component rotates to the position corresponding to the reflection channel. At this time, a light source of a specific wavelength is emitted by the excitation light source component, and the light source is reflected by the reflection component, enters the reflection channel, and then enters the fluorescence component of the same specific wavelength through the light through hole for fluorescence illumination.

[0025] In one embodiment, the fluorescence component includes: a fluorescence component base, a fluorescence reflector, and a cutoff film; the fluorescence component base is fixedly arranged on the fluorescence rotating part, the fluorescence reflector is obliquely arranged in the fluorescence component base, and the cutoff film is arranged on the fluorescence component base; the light through hole includes a first light through hole and a second light through hole, the first light through hole is arranged on the fluorescence rotating part, the second light through hole is arranged on the fluorescence component base, and the first light through hole is communicated with the second light through hole.

[0026] In the above technical solution, when the excitation light source emitted by the excitation light source component is projected onto the reflection component, after being reflected by the reflection component, it is projected from the reflection channel onto the first light through hole on the fluorescence rotating part, and then projected onto the fluorescence reflector through the second light through hole on the fluorescence component base, and after being reflected by the fluorescence reflector, it is emitted through the cutoff film to achieve fluorescence illumination.

[0027] In one embodiment, the fluorescence excitation device further includes: a second limiting module; the second limiting module is used to limit the fluorescence rotating part when the fluorescence rotating part rotates.

[0028] In the above technical solution, the second limiting module can limit the fluorescence rotating part when the fluorescence rotating part rotates to prevent the fluorescence rotating part from rotating.

[0029] In one embodiment, the second limiting module includes: a second mounting base and a second limiting member; the second mounting base is arranged on the base, the second limiting member is rotatably arranged on the second mounting base, and a second groove matching the second limiting member is arranged on the fluorescence rotating part.

[0030] In the above technical solution, the structure of the second limiting module is simple and it is easier to achieve the limiting effect.

[0031] In a second aspect, the present application provides a sample processing device, including the fluorescence excitation device according to any one of the embodiments of the first aspect of the present application.

[0032] In the above technical solution, the sample processing device can be applied in the medical field. Through the sample processing device, various samples can be realized, such as the detection of sample reagents such as serum and nucleic acid, and the application is more extensive. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present application, and therefore should not be regarded as limiting the scope. For those of ordinary skill in the art, other related drawings can be obtained based on these drawings without creative efforts.

[0034] Figure 1 Schematic diagram of the overall structure of the fluorescence excitation device provided by an embodiment of the present application;

[0035] Figure 2 Front view of the fluorescence excitation device provided by an embodiment of the present application;

[0036] Figure 3 Left view of the fluorescence excitation device provided by an embodiment of the present application;

[0037] Figure 4 For Figure 3 Cross-sectional view taken along the line A-A in

[0038] Figure 5 Top view of the fluorescence excitation device provided by an embodiment of the present application;

[0039] Figure 6 Bottom view of the fluorescence excitation device provided by an embodiment of the present application;

[0040] Figure 7 Explosion diagram of the fluorescence excitation device provided by an embodiment of the present application;

[0041] Figure 8 Schematic diagram of the structure of the excitation light source assembly provided by an embodiment of the present application;

[0042] Figure 9 Schematic diagram of the structure of the reflection module provided by an embodiment of the present application;

[0043] Figure 10 Schematic diagram of the structure of the first limiting module provided by an embodiment of the present application;

[0044] Figure 11 Schematic diagram of the structure of the second limiting module provided by an embodiment of the present application.

[0045] ICON:

[0046] 1 - Fluorescence excitation device; 100 - Base; 200 - Excitation light source module; 210 - Excitation light source assembly; 211 - Light source base; 2111 - Light source excitation channel; 212 - Excitation light source; 213 - Excitation sheet; 214 - Heat dissipation assembly; 300 - Reflection module; 310 - Reflection channel; 320 - First drive assembly; 330 - Reflection assembly; 331 - Reflection rotating part; 3311 - First groove; 332 - Reflecting piece; 333 - Bearing; 400 - Fluorescence module; 410 - Light through hole; 411 - First light through hole; 412 - Second light through hole; 420 - Second drive assembly; 430 - Fluorescence rotating part; 431 - Second groove; 440 - Fluorescence assembly; 441 - Fluorescence assembly base; 442 - Fluorescence reflector; 443 - Cut-off sheet; 500 - First limit module; 510 - First mounting base; 520 - First limit piece; 530 - Elastic piece; 600 - Second limit module; 610 - Second mounting base; 620 - Second limit piece; 700 - First optoelectronic detection assembly; 710 - First optoelectronic switch; 720 - First light blocking piece; 800 - Second optoelectronic detection assembly; 810 - Second optoelectronic switch; 820 - Second light blocking piece. Detailed implementation mode

[0047] The terms "first", "second", "third", etc. are only used for differential description, do not represent the arrangement serial number, nor can they be understood as indicating or implying relative importance.

[0048] In addition, the terms "horizontal", "vertical", "overhanging", etc. do not mean that the components are required to be absolutely horizontal or overhanging, but can be slightly inclined. For example, "horizontal" only means that its direction is more horizontal relative to "vertical", and does not mean that the structure must be completely horizontal, but can be slightly inclined.

[0049] In the description of the present application, it should be noted that the orientation or positional relationship indicated by the terms "inner", "outer", "left", "right", "upper", "lower", etc. is based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship in which the product of this application is usually placed when in use. It is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation to the present application.

[0050] In the description of the present application, unless otherwise clearly specified and limited, the terms "set", "installed", "connected", "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, and can be the communication inside two elements.

[0051] The technical solution of the embodiment of the present application will be described below with reference to the accompanying drawings.

[0052] Please refer to Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 、 Figure 5 、 Figure 6 and Figure 7 As shown, the present application provides a fluorescence excitation device 1, including: a base 100, an excitation light source module 200, a reflection module 300, and a fluorescence module 400; the excitation light source module 200 is fixedly arranged on the base 100; the reflection module 300 is rotatably arranged on the base 100, and the reflection module 300 has a reflection channel 310; the fluorescence module 400 is rotatably arranged on the base 100, and a light through hole 410 is arranged on the fluorescence module 400; when the reflection module 300 rotates to correspond to the excitation light source module 200, and the fluorescence module 400 rotates to correspond to the reflection channel 310, the wavelength of the fluorescence module 400 corresponds to the wavelength of the excitation light source module 200. The excitation light emitted by the excitation light source module 200 is reflected by the reflection module 300, then emitted from the reflection channel 310, and enters the fluorescence module 400 through the light through hole 410.

[0053] In this embodiment, the excitation light source module 200 and the fluorescence module 400 can be separately controlled, which is more convenient for independent installation and debugging. The excitation light source module 200 is fixedly arranged, and the rotation of the reflection module 300 is used to replace the movement of the excitation light source, so that the wire harness of the excitation light source module 200 will not be bent as the light source moves, which is more convenient for fixing. When the reflection module 300 rotates to correspond to the excitation light source module 200, by rotating the fluorescence module 400, the fluorescence module 400 with a wavelength corresponding to that of the excitation light source module 200 rotates until the light through hole 410 corresponds to the reflection channel 310 of the reflection module 300. At this time, the excitation light emitted by the excitation light source module 200 is emitted to the reflection module 300, reflected by the reflection module 300 and then emitted from the reflection passage 310, and enters the fluorescence module 400 through the light through hole 410, realizing fluorescence illumination. Fluorescence can be used to perform fluorescence detection and analysis on the sample to be detected.

[0054] In some embodiments, the excitation light source module 200 and the reflection module 300 can be disposed on one side of the base 100, for example, on the bottom surface of the base 100, and the fluorescence module 400 can be disposed on the other side of the base, for example, on the upper surface of the base 100. In some other embodiments, the excitation light source module 200, the reflection module 300, and the fluorescence module 400 can all be disposed on the same side of the base 100. The fluorescence module 400 can be fixedly mounted on the base 100 through a mounting bracket, such that the fluorescence module 400 is located above the excitation light source module 200 and the reflection module 300. The excitation light emitted by the excitation light source module 200 is directed towards the reflection module 300, reflected by the reflection module 300, and then emitted through the reflection channel 310, and enters the fluorescence module 400 through the light through-hole 410 to achieve fluorescence illumination.

[0055] Further, please refer to Figure 4 As shown, the excitation light source module 200 includes: at least one excitation light source component 210, and the excitation light source components 210 are arranged at intervals around the reflection module 300 as the center.

[0056] The number of the excitation light source components 210 can be determined according to the wavelength parameters required for the experiment. As an example, one excitation light source component 210 can realize the excitation of an excitation light source of a specific wavelength. In other embodiments, multiple excitation light source components 210 can also all realize the excitation of an excitation light source of a specific wavelength.

[0057] In this embodiment, the excitation light source components 210 can be set to 5, and each excitation light source component 210 can realize the excitation of an excitation light source of a specific wavelength. Therefore, the excitation light source module 200 can realize the excitation of 5 excitation light sources of specific wavelengths. The 5 excitation light source components 210 in this embodiment are arranged at intervals in a circular array around the reflection module 300 as the center. The adjacent excitation light source components 210 can be arranged at equal intervals or at unequal intervals around.

[0058] Further, please refer to Figure 4 and Figure 8 As shown, the excitation light source component 210 includes: a light source base 211, an excitation light source 212, and an excitation sheet 213; the light source base 211 is fixedly disposed on the base 100, a light source excitation channel 2111 is provided in the light source base 211, the excitation light source 212 is disposed in the light source base 211 at the incident end of the light source excitation channel 2111, and the excitation sheet 213 is disposed in the light source base 211 and at the exit end of the light source excitation channel 2111.

[0059] In some embodiments, the excitation light source 212 can be an LED light source. The excitation light emitted by the excitation light source 212 is directed towards the excitation sheet 213 through the light source excitation channel 2111, and then towards the reflection module 300 after passing through the excitation sheet 213.

[0060] In some embodiments, a heat dissipation component 214 may also be provided at a position on the light source base 211 close to the excitation light source 212. The heat dissipation component 214 is used to dissipate heat from the excitation light source 212. As an example, the heat dissipation component 214 is a heat dissipation block made of copper. The heat conduction of copper is faster than that of air. When the heat dissipation component 214 contacts the air, the heat dissipation efficiency can be improved.

[0061] Further, please refer to Figure 6 and Figure 9 As shown, the reflection module 300 includes: a first driving component 320 and a reflection component 330; the first driving component 320 is used to drive the reflection component 330 to rotate so that the reflection component 330 corresponds to the excitation light source module 200.

[0062] In this embodiment, according to the light source of a specific wavelength required for the fluorescence experiment, the first driving component 320 drives the reflection component 330 to rotate so that the reflection component 330 corresponds to the set excitation light source component 210 of a specific wavelength. The excitation light of a specific wavelength emitted by the excitation light source component 210 is directed to the reflection component 330, and then the reflection component 330 reflects the excitation light source.

[0063] When it is necessary to switch the excitation light source of a different wavelength, the first driving component 320 can drive the reflection component 330 to rotate so that the reflection component 330 rotates to the position where the excitation light source component 210 of the required specific wavelength is located, and then the reflection component 330 reflects the excitation light source.

[0064] In some embodiments, the first driving component 320 may be one of a pulley transmission component, a gear transmission component, and a cam transmission component. In this embodiment, the first driving component 320 is a pulley transmission component, including a motor, a driving pulley, a driven pulley, and a belt. The output shaft of the motor is connected to the driving pulley. The driven pulley is arranged on the reflection component 330. The belt is connected between the driving pulley and the driven pulley. By driving the driving pulley to rotate by the motor, the driven pulley is driven to rotate under the transmission of the belt, and then the reflection component 330 is driven to rotate.

[0065] Further, please refer to Figure 9 As shown, the reflection component 330 includes: a reflection rotating part 331 and a reflector 332; the first driving component 320 is connected to the reflection rotating part 331. The reflector 332 is arranged on the reflection rotating part 331. The reflection rotating part 331 is rotatably arranged on the base 100.

[0066] As described above, taking the excitation light source module 200 and the reflection module 300 being disposed on the bottom surface of the base 100 and the first driving assembly 320 being a pulley transmission assembly as an example, the driven pulley of the first driving assembly 320 can be disposed on the reflection rotating part 331, the reflecting member 332 is disposed at the bottom of the reflection rotating part 331, and the reflection rotating part 331 can pass through the base 100 and is rotatably fixed on the upper surface of the base 100 through a bearing 333. When the motor of the first driving assembly 320 drives the driving pulley to rotate, under the transmission action of the belt, the driven pulley is driven to rotate, and then the reflection rotating part 331 is driven to rotate, and further the reflecting member 332 is driven to rotate, so that the reflecting member 332 rotates to face the position where the excitation light source assembly 210 of the required specific wavelength is located.

[0067] In some embodiments, the reflection channel 310 penetrates through the reflection rotating part 331 and communicates with the reflecting member 332. The middle part of the reflection rotating part 331 has the reflection channel 310 for the excitation light source to pass through. The reflecting member 332 can be a dichroic mirror disposed at an angle of 45°, and the dichroic mirror is used to adjust the direction of the excitation light source emitted by the excitation light source assembly 210 to the reflection channel 310.

[0068] Furthermore, in order to limit the rotation of the reflection rotating part 331, the fluorescence excitation device 1 of the present application further includes: a first limiting module 500; the first limiting module 500 is used to limit the reflection rotating part 331 when the reflection rotating part 331 rotates.

[0069] Specifically, please refer to Figure 10 As shown, the first limiting module 500 includes: a first mounting base 510, a first limiting member 520 and an elastic member 530; the first mounting base 510 is disposed on the base 100, the first limiting member 520 is rotatably disposed on the first mounting base 510, one end of the elastic member 530 is connected to the first mounting base 510, and the other end is connected to the first limiting member 520; a first groove 3311 matching the first limiting member 520 is provided on the reflection rotating part 331.

[0070] In this embodiment, the first mounting base 510 can be fixedly mounted on the base 100 through bolts, and the first limiting member 520 can be rotatably mounted on the first mounting base 510 through a rotating bearing. As an example, the first limiting member 520 can be a bearing that can rotate on the first mounting base 510, and the surface of the bearing is circular and matches the shape of the first groove 3311.

[0071] Furthermore, the first groove 3311 can be provided on the bearing 333 through which the reflection rotating part 331 passes through the base 100 and is fixed on the upper surface of the base 100, and the number of the first grooves 3311 can be the same as the number of the excitation light source assemblies 210.

[0072] When it is necessary to use the excitation light source assembly 210 of the first specific wavelength for light source excitation to achieve fluorescence irradiation of the first specific wavelength, the reflection and rotation part 331 is driven by the first driving assembly 320 to rotate clockwise or counterclockwise to a position corresponding to the excitation light source assembly 210 of the first specific wavelength. For example, the excitation light source assembly 210 of the first specific wavelength can be called the first excitation light source assembly 210. At this time, the first first groove 3311 on the bearing 333 of the reflection and rotation part 331 contacts the first limiting part 520. Under the elastic force of the elastic part 530, the surface of the first limiting part 520 is pushed to closely adhere to the first first groove 3311, so that the first limiting part 520 abuts against and clamps the first groove 3311, enabling the first limiting module 500 to play a role in limiting the reflection and rotation part 331 and preventing the reflection and rotation part 331 from continuing to rotate.

[0073] When it is necessary to use the excitation light source assembly 210 of the second specific wavelength for light source excitation to achieve fluorescence irradiation of the second specific wavelength, the first driving assembly 320 continues to drive the reflection and rotation part 331 to rotate clockwise or counterclockwise. For example, the excitation light source assembly 210 of the second specific wavelength can be called the second excitation light source assembly 210. Under the rotational force of the reflection and rotation part 331, the bearing 333 of the reflection and rotation part 331 pushes and squeezes the first limiting part 520. After the first limiting part 520 receives the pushing and squeezing force, the elastic part 530 is compressed and in a compressed state. When the first driving assembly 320 continues to drive the reflection and rotation part 331 to rotate to a position corresponding to the second excitation light source assembly 210, the second first groove 3311 on the bearing 333 of the reflection and rotation part 331 contacts the surface of the first limiting part 520. Under the elastic restoring force of the elastic part 530, the elastic part 530 pushes the surface of the first limiting part 520 to closely adhere to the second first groove 3311, so that the first limiting part 520 abuts against and clamps the first groove 3311, and the first limiting module 500 plays a role in limiting the reflection and rotation part 331 and preventing the reflection and rotation part 331 from continuing to rotate.

[0074] When it is necessary to use the excitation light source assembly 210 of the third specific wavelength for light source excitation to achieve fluorescence irradiation of the third specific wavelength, and so on.

[0075] Further, please refer to Figure 4 、 Figure 5 and Figure 7As shown, the fluorescence module 400 includes: a second driving component 420, a fluorescence rotating part 430, and at least one fluorescence component 440; the fluorescence components 440 are arranged on the fluorescence rotating part 430 at intervals in a ring shape, and the second driving component 420 is connected to the fluorescence rotating part 430 for driving the fluorescence rotating part 430 to rotate so as to drive the fluorescence components 440 to rotate, so that any one of the fluorescence components 440 corresponds to the reflection channel 310.

[0076] In this embodiment, according to the light source of a specific wavelength required for the fluorescence experiment, the reflection component 330 is driven to rotate by the first driving component 320, so that the reflection component 330 corresponds to the excitation light source component 210 of the set first specific wavelength. At the same time, the fluorescence rotating part 430 is driven to rotate by the second driving component 420, so that the fluorescence component 440 with the same first specific wavelength as the excitation light source component 210 rotates to a position corresponding to the reflection channel 310. When the excitation light of the first specific wavelength emitted by the excitation light source component 210 of the first specific wavelength irradiates the reflection component 330, and then the reflection component 330 reflects the excitation light source, the reflected light source irradiates into the first specific wavelength fluorescence component 440 through the light through hole 410 on the fluorescence module 400 to achieve fluorescence illumination.

[0077] Therefore, it can be understood that when it is necessary to switch the excitation light sources of different wavelengths, the reflection component 330 can be driven to rotate by the first driving component 320, so that the reflection component 330 rotates to the position where the excitation light source component 210 of the required specific wavelength is located. At the same time, the fluorescence rotating part 430 is driven to rotate by the second driving component 420, so that the fluorescence component 440 with the same specific wavelength as the excitation light source component 210 rotates to a position corresponding to the reflection channel 310. At this time, a light source of a specific wavelength is emitted by the excitation light source component 210, the light source is reflected by the reflection component 330, enters the reflection channel 310, and then enters the fluorescence component 440 of the same specific wavelength through the light through hole 410 for fluorescence illumination.

[0078] In some embodiments, the second driving component 420 can be one of a pulley transmission component, a gear transmission component, and a cam transmission component. In this embodiment, the second driving component 420 is a pulley transmission component, including a motor, a driving pulley, a driven pulley, and a belt. The output shaft of the motor is connected to the driving pulley, the driven pulley is arranged at the bottom of the fluorescence rotating part 430, and the belt is connected between the driving pulley and the driven pulley. The driving pulley is driven to rotate by the motor, and under the driving action of the belt, the driven pulley is driven to rotate, and then the fluorescence rotating part 430 is driven to rotate.

[0079] In some embodiments, the fluorescence rotating part 430 can be a circular turntable structure, and multiple fluorescence components 440 can be provided and distributed at intervals in a circumferential array. In this embodiment, 6 fluorescence components 440 can be provided, and the wavelengths of 5 of the fluorescence components 440 correspond one-to-one with the wavelengths of 5 excitation light source components 210, and 1 fluorescence component 440 is reserved for experimental backup.

[0080] Further, please refer to Figure 4 and Figure 7 , the fluorescence component 440 includes: a fluorescence component base 441, a fluorescence reflector 442, and a cut-off film 443; the fluorescence component base 441 is fixedly arranged on the fluorescence rotating part 430, the fluorescence reflector 442 is obliquely arranged in the fluorescence component base 441, and the cut-off film 443 is arranged on the fluorescence component base 441; the light through hole 410 includes a first light through hole 411 and a second light through hole 412, the first light through hole 411 is arranged on the fluorescence rotating part 430, the second light through hole 412 is arranged on the fluorescence component base 441, and the first light through hole 411 communicates with the second light through hole 412.

[0081] In some embodiments, the number of the first light through holes 411 is the same as the number of the second light through holes 412 and is the same as the number of the fluorescence components 440, all being 6.

[0082] In some embodiments, the fluorescence reflector 442 can be obliquely arranged at an angle of 45° in the fluorescence component base 441. When the excitation light source emitted by the excitation light source component 210 is incident on the reflection component 330, after being reflected by the reflection component 330, it is incident on the first light through hole 411 on the fluorescence rotating part 430 through the reflection channel 310, and then is incident on the fluorescence reflector 442 through the second light through hole 412 on the fluorescence component base 441. After being reflected by the fluorescence reflector 442, it is emitted through the cut-off film 443 to achieve fluorescence illumination.

[0083] Further, in order to limit the rotation of the fluorescence rotating part 430, the fluorescence excitation device 1 of the present application further includes: a second limiting module 600; the second limiting module 600 is used to limit the fluorescence rotating part 430 when the fluorescence rotating part 430 rotates.

[0084] Specifically, please refer to Figure 11 as shown, the second limiting module 600 includes: a second mounting base 610 and a second limiting member 620; the second mounting base 610 is arranged on the base 100, the second limiting member 620 is rotatably arranged on the second mounting base 610, and a second groove 431 matching the second limiting member 620 is arranged on the fluorescence rotating part 430.

[0085] In this embodiment, the second mounting base 610 can be fixedly mounted on the base 100 by bolts, and the second limiting member 620 can be mounted on the second mounting base 610 through a rotating bearing. As an example, the second limiting member 620 can be a bearing capable of rotating on the second mounting base 610, and the surface of the bearing is circular, matching the shape of the second groove 431.

[0086] In some embodiments, the number of the second grooves 431 can be the same as the number of the fluorescent components 440.

[0087] When it is necessary to use the first excitation light source component 210 with a specific wavelength for light source excitation to achieve fluorescence irradiation with the first specific wavelength, the reflection rotating part 331 is driven by the first driving component 320 to rotate clockwise or counterclockwise to a position corresponding to the first excitation light source component 210 with the specific wavelength. At this time, the first first groove 3311 on the bearing 333 of the reflection rotating part 331 contacts the first limiting member 520. Under the elastic force of the elastic member 530, the surface of the first limiting member 520 is pushed to closely adhere to the first first groove 3311, so that the first limiting module 500 plays a role in limiting the reflection rotating part 331 and preventing the reflection rotating part 331 from continuing to rotate.

[0088] At the same time, the fluorescence rotating part 430 is driven to rotate by the second driving component 420, so that the fluorescence component 440 with the same first specific wavelength as the excitation light source component 210 rotates to a position corresponding to the reflection channel 310. The first light through hole 411 and the second light through hole 412 are respectively communicated with the reflection channel 310. At this time, the first second groove 431 on the fluorescence rotating part 430 contacts the second limiting member 620, and the surface of the second limiting member 620 closely adheres to the first second groove 431, so that the second limiting member abuts against and clamps the second groove 431, thereby enabling the second limiting module 600 to play a role in limiting the fluorescence rotating part 430 and preventing the fluorescence rotating part 430 from continuing to rotate.

[0089] After the first limiting module 500 limits and fixes the reflection rotating part 331 and the second limiting module 600 limits and fixes the fluorescence rotating part 430, it can prevent the reflection rotating part 331 and the fluorescence rotating part 430 from rotating during the fluorescence irradiation experiment and affecting the experimental results of the fluorescence irradiation.

[0090] Next, an excitation light source is emitted by the excitation light source 212 in the excitation light source assembly 210 of the first specific wavelength. The light source is projected through the light source excitation channel 2111 onto the excitation sheet 213, and then onto the reflection assembly 330 through the excitation sheet 213. The reflection assembly 330 reflects the excitation light source to adjust the direction of the excitation light source to the reflection channel 310. The excitation light source passing through the reflection channel 310 passes through the first light through hole 411 on the fluorescence rotation part 430 respectively, and then passes through the second light through hole 412 on the fluorescence assembly base 441 and is projected onto the fluorescence reflector 442 of the first specific wavelength. After being reflected by the fluorescence reflector 442, it is emitted through the cutoff sheet 443 to achieve fluorescence illumination of the first specific wavelength. Therefore, according to the needs of fluorescence experiments, the excitation light source module 200 and the fluorescence module 400 can be freely switched to meet the experimental requirements of different fluorescence illuminations.

[0091] During the fluorescence illumination experiment, in order to accurately record the rotation angle and orientation of the reflection module 300, as well as the rotation angle and orientation of the fluorescence module 400. In some embodiments, the fluorescence excitation device 1 is further provided with a first photoelectric detection component 700 and a second photoelectric detection component 800.

[0092] In some embodiments, please refer to Figure 9 , the first photoelectric detection component 700 may include: a first photoelectric switch 710 and a first light blocking sheet 720 cooperating with the first photoelectric switch 710. The first photoelectric switch 710 is arranged on the lower surface of the base 100, and the first light blocking sheet 720 is arranged on the reflection rotation part 331.

[0093] Please refer to Figure 11 , the second photoelectric detection component 800 may include: a second photoelectric switch 810 and a second light blocking sheet 820 cooperating with the second photoelectric switch 810. The second photoelectric switch 810 is arranged on the upper surface of the base 100, and the second light blocking sheet 820 is arranged on the fluorescence rotation part 430.

[0094] In this embodiment, by setting the first photoelectric detection component 700, the rotation position of the reflection component 330 in the reflection module 300 can be accurately identified. When the first driving component 320 drives the reflection rotation part 331 to rotate by 0 - 360°, the first light blocking sheet 720 is driven to rotate. When the first light blocking sheet 720 rotates to the position where the first photoelectric switch 710 is located, it is detected by the first photoelectric switch 710, and the first photoelectric switch 710 sends the detected signal to the main control module, and the main control module controls the stop or start of the first driving component 320.

[0095] Similarly, by setting the second photoelectric detection component 800, the rotation position of the fluorescent component 440 in the fluorescence module 400 can be accurately identified. When the second driving component 420 drives the fluorescence rotating part 430 to rotate by 0 to 360°, the second light shielding sheet 820 is driven to rotate. When the second light shielding sheet 820 rotates to the position where the second photoelectric switch 810 is located, it is detected by the second photoelectric switch 810, and the second photoelectric switch 810 sends the detected signal to the main control module, and the main control module controls the stop or start of the second driving component 420.

[0096] In some embodiments, the main control module may include: a power supply unit, a human-machine interaction interface, a communication unit, a processor, and a control unit. The power supply unit may be an external power supply or a storage battery. The human-machine interaction interface may be computer input and output devices such as a display screen, a keyboard, a touch screen, buttons, knobs, a speaker, and an LED lamp, which are used to input instructions and read information, so as to achieve human-machine interaction and information intercommunication. The communication unit may be a transceiver, and the control unit may be a microcontroller (Microcontroller Unit, abbreviated as: MCU). The main control module processes the information fed back by the human-machine interaction interface and the communication unit through the processor, and controls the stop or start of the first driving component 320 and the second driving component 420 through the control unit.

[0097] This application also provides a sample processing device, including the Figures 1 - 4 fluorescence excitation device 1 as shown. For example, the fluorescence excitation device 1 may be installed or integrated on the sample processing device, or used in cooperation with the sample processing device. A pipetting module may be configured on the sample processing device to transfer the sample to the position where the fluorescence excitation device 1 is located for fluorescence detection. In addition to being applicable to the sample processing device, the fluorescence excitation device 1 of this application can also be installed in a small pipetting workstation.

[0098] In some embodiments, the sample processing device may be applied in the medical field for detecting sample reagents such as serum and nucleic acids. For example, DNA, RNA, or protein in a serum sample or a nucleic acid sample is excited by the excitation light, so that the sample reagent enters the excited state, and the DNA, RNA, or protein therein will emit corresponding emission light, and finally enter the excitation detection component, and the light intensity of the fluorescent substance in the sample reagent is measured through the excitation detection component.

[0099] It should be noted that, without conflict, the features in the embodiments of this application can be combined with each other.

[0100] The above are only the preferred embodiments of the present application and are not intended to limit the present application. For those skilled in the art, various changes and modifications can be made to the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included within the protection scope of the present application.

Claims

1. A fluorescence excitation device, characterized in that, Comprising: A base; An excitation light source module, fixedly provided on the base; A reflection module, rotatably provided on the base, the reflection module having a reflection channel; A fluorescence module, rotatably provided on the base, the fluorescence module being provided with a light through-hole; When the reflection module rotates to correspond to the excitation light source module, and the fluorescence module rotates to correspond to the reflection channel, so that the wavelength of the fluorescence module corresponds to the wavelength of the excitation light source module, the excitation light emitted by the excitation light source module is reflected by the reflection module and then emitted through the reflection channel and enters the fluorescence module through the light through-hole.

2. The fluorescence excitation device according to claim 1, wherein The excitation light source module includes: at least one excitation light source component, and the excitation light source components are arranged at intervals around the reflection module as the center.

3. The fluorescence excitation device according to claim 2, characterized in that The excitation light source component includes: a light source base, an excitation light source and an excitation sheet; the light source base is fixedly provided on the base, a light source excitation channel is provided in the light source base, the excitation light source is provided at the incident end of the light source excitation channel in the light source base, and the excitation sheet is provided in the light source base and at the exit end of the light source excitation channel.

4. The fluorescence excitation device according to claim 1, characterized in that, The reflection module includes: a first driving component and a reflection component; the first driving component is used to drive the reflection component to rotate so that the reflection component corresponds to the excitation light source module.

5. The fluorescence excitation device according to claim 4, characterized in that, The reflection component includes: a reflection rotating part and a reflector; the first driving component is connected to the reflection rotating part, the reflector is provided on the reflection rotating part, and the reflection rotating part is rotatably provided on the base.

6. The fluorescence excitation device according to claim 5, characterized in that, The reflection channel penetrates through the reflection rotating part and communicates with the reflector.

7. The fluorescence excitation device according to claim 5, wherein, The fluorescence excitation device further includes: a first limiting module; the first limiting module is used to limit the reflection rotating part when the reflection rotating part rotates.

8. The fluorescence excitation device according to claim 7, characterized in that, The first limiting module includes: a first mounting base, a first limiting member and an elastic member; the first mounting base is provided on the base, the first limiting member is rotatably provided on the first mounting base, one end of the elastic member is connected to the first mounting base, and the other end is connected to the first limiting member; a first groove matching the first limiting member is provided on the reflection rotating part.

9. The fluorescence excitation device according to claim 1, characterized in that, The fluorescence module includes: a second driving component, a fluorescence rotating part and at least one fluorescence component; the fluorescence components are arranged at intervals around the fluorescence rotating part, the second driving component is connected to the fluorescence rotating part and is used to drive the fluorescence rotating part to rotate to drive the fluorescence components to rotate so that any one of the fluorescence components corresponds to the reflection channel.

10. The fluorescence excitation device according to claim 9, characterized in that, The fluorescence component includes: a fluorescence component base, a fluorescence reflector and a cut-off sheet; the fluorescence component base is fixedly provided on the fluorescence rotating part, the fluorescence reflector is obliquely provided in the fluorescence component base, the cut-off sheet is provided on the fluorescence component base; the light through-hole includes a first light through-hole and a second light through-hole, the first light through-hole is provided on the fluorescence rotating part, the second light through-hole is provided on the fluorescence component base, and the first light through-hole communicates with the second light through-hole.

11. The fluorescence excitation device according to claim 10, wherein The fluorescence excitation device further includes: a second limiting module; the second limiting module is configured to limit the fluorescence rotating part when the fluorescence rotating part rotates.

12. The fluorescence excitation device according to claim 11, wherein The second limiting module includes: a second mounting base and a second limiting member; the second mounting base is disposed on the base, the second limiting member is rotatably disposed on the second mounting base, and a second groove matching the second limiting member is provided on the fluorescence rotating part.

13. A sample processing device, characterized in that, It includes the fluorescence excitation device according to any one of claims 1 to 12.

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