Device for detecting biological activity of psylla chinensis on chemical pesticide
By designing an automated pesticide bioactivity detection device for pesticides, the cumbersome experimental process and the accuracy of results were solved, and multi-concentration pesticide experiments of multiple groups of pesticides under the same conditions were achieved, improving the experimental efficiency and accuracy.
Patent Information
- Application Number
- CN202421864362.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-02
- Publication Date
- 2025-07-08
- Estimated Expiration
- 2034-08-02
AI Technical Summary
In the prior art, the experimental process of detecting the virulence of pesticides on the biological activity of chemical pesticides is cumbersome and is easily affected by the prolonged experimental time, resulting in a decrease in the accuracy of experimental results.
A device including a box, a culture chamber and a vegetation chamber is designed, equipped with an electric telescopic rod, a ventilation mechanism, a humidification mechanism, a temperature and humidity measurement mechanism and a pharmaceutical application mechanism. Automatic operation is achieved through the controller to ensure that multiple groups of pesticide experiments are carried out under the same conditions and reduce system errors.
The experimental process is simplified, the experimental time is shortened, the accuracy and consistency of experimental results are improved, and the error caused by differences in culture conditions is reduced.
Smart Images
Figure CN223078324U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of bioactivity determination of pesticides for Psylla pyrisuga, in particular to a device for detecting the bioactivity of chemical pesticides against Psylla pyrisuga. Background Art
[0002] Psylla pyrisuga is an animal of the family Psyllidae in the order Hemiptera and is also one of the main pests of pear trees in China. The adults and nymphs directly damage the buds, leaves and tender branches by sucking their juices, and secrete mucus, attracting miscellaneous bacteria, causing indirect damage to the leaves, resulting in brown spots and early defoliation. At the same time, the fruits are polluted, seriously affecting the yield and quality of pears. Therefore, the prevention and control of Psylla pyrisuga is very important.
[0003] Using pesticides to control Psylla pyrisuga is a common method. The control effect of pesticides on Psylla pyrisuga needs to be determined by toxicity tests. In the prior art, when conducting toxicity determination experiments on Psylla pyrisuga, different concentrations of pesticides need to be used for multiple experiments. The experimental process is relatively cumbersome. Moreover, as the experimental time extends, it is very likely that changes will occur in the control group and nymphs under different treatments, thus affecting the accuracy of the experimental results and leading to a certain degree of systematic error. Therefore, a device for detecting the bioactivity of chemical pesticides against Psylla pyrisuga is proposed to solve the above problems. Summary of the Utility Model
[0004] The purpose of the utility model is to provide a device for detecting the bioactivity of chemical pesticides against Psylla pyrisuga, so as to solve the problems existing in the above-mentioned prior art, make the toxicity determination experiment more convenient, shorten the experimental time at the same time, and reduce the influence on the experimental results.
[0005] To achieve the above purpose, the utility model provides the following scheme: The utility model provides a device for detecting the bioactivity of chemical pesticides against Psylla pyrisuga, including:
[0006] A box body, in which a culture chamber and a number of bioassay chambers are arranged.
[0007] A culture component, the culture component includes a culture dish. A number of electric telescopic rods are fixedly connected in the culture chamber. The output end of the electric telescopic rod is fixedly connected with a bottom plate. The culture dish is arranged on the bottom plate. A first through hole is arranged between the bioassay chamber and the culture chamber. The bottom plate is correspondingly arranged with the first through hole. A ventilation mechanism, a humidification mechanism and a temperature and humidity measuring mechanism are arranged in the culture chamber. A first illuminating lamp is fixedly connected in the culture chamber.
[0008] A bioassay component, the bioassay component includes a number of drug application mechanisms. The drug application mechanisms are arranged on the bioassay chambers. The drug application mechanisms are provided with drug spray nozzles and medicine adding funnels. The drug application mechanisms are communicated with a pressure tank, and the pressure tank is communicated with a gas source.
[0009] A controller, which is fixedly connected to the outer wall of the box body. The electric telescopic rod, the ventilation mechanism, the humidification mechanism, the temperature and humidity measurement mechanism, the first lighting lamp, and the drug application mechanism are all electrically connected to the controller.
[0010] Preferably, a horizontal plate and several vertical plates are fixedly connected inside the box body. The vertical plates are fixedly connected to the horizontal plate. The horizontal plate and the vertical plates divide the inside of the box body into the culture chamber and the bioassay chamber. The first through hole is opened on the horizontal plate.
[0011] Preferably, a lower retaining ring is fixedly connected to the bottom plate, an upper retaining ring is fixedly connected inside the first through hole. The lower retaining ring and the upper retaining ring are arranged corresponding to each other. A sealing ring is fixedly connected to the lower retaining ring, a groove is opened on the upper retaining ring, and the sealing ring and the groove are arranged corresponding to each other.
[0012] Preferably, the drug application mechanism includes a medicine cylinder, which is arranged in the culture chamber and extends out of the box body. A first connecting pipe is communicated with the medicine cylinder, a manual valve is installed on the first connecting pipe, a medicine adding funnel is fixedly connected to the first connecting pipe. A second connecting pipe is communicated with the medicine cylinder, one end of the second connecting pipe far away from the medicine cylinder is communicated with the air pressure tank, the air pressure tank is communicated with a gas source, an electromagnetic valve is installed on the second connecting pipe. A third connecting pipe is communicated with the medicine cylinder, a drug spray head is fixedly connected to the third connecting pipe, and the electromagnetic valve is electrically connected to the controller.
[0013] Preferably, the temperature and humidity measurement mechanism includes several temperature and humidity sensors, which are fixedly connected in the culture chamber, and the temperature and humidity sensors are electrically connected to the controller.
[0014] Preferably, the ventilation mechanism includes a hot air blower, a blower, and a ventilation pipe. The ventilation pipe extends into the culture chamber, several air outlets are opened on the ventilation pipe, the hot air blower and the blower are both communicated with the ventilation pipe, and the hot air blower and the blower are both electrically connected to the controller.
[0015] Preferably, the humidification mechanism includes several water mist spray heads, which are installed on a water pipe. The water mist spray heads are communicated with the water pipe. The water mist spray heads are located in the culture chamber. The water pipe extends out of the box body, and the water pipe is communicated with a water pump. The water inlet end of the water pump is communicated with a water source.
[0016] Preferably, a second through hole is formed in the side wall of the box body. The second through hole is located in the culture chamber. A baffle is arranged on the second through hole. The baffle is rotatably connected to the side wall of the box body. A sleeve is fixedly connected to the side wall of the box body. A rotating shaft is rotatably connected in the sleeve. The baffle is fixedly connected to the rotating shaft. A first magnet is fixedly connected to the baffle. A second magnet is fixedly connected to the side wall of the box body. The first magnet and the second magnet are arranged corresponding to each other.
[0017] Preferably, a fixing seat is fixedly connected to the bottom plate. The culture dish is clamped in the fixing seat. A plurality of second lighting lamps are fixedly connected in the bioassay chamber. The second lighting lamps are electrically connected to the controller. A first access opening is formed in the bioassay chamber. A second access opening is formed in the culture chamber. A sealing door one is rotatably connected to the first access opening. A sealing door two is rotatably connected to the second access opening.
[0018] The utility model discloses the following technical effects: In this device, the culture chamber is used for culturing Psylla chinensis, the bioassay chamber is used for detecting the effect of chemical pesticides on Psylla chinensis, and the culture dish is used for placing Psylla chinensis. In practical applications, Psylla chinensis is placed on the pear tree leaves, and the pear tree leaves are placed in the culture dish. The electric telescopic rod is used to raise or lower the culture dish, so that the culture dish enters the bioassay chamber from the culture chamber. After the electric telescopic rod drives the bottom plate to rise, the bottom plate will enter the first through hole, so that the bioassay chamber becomes a relatively airtight space. The ventilation mechanism is used to ventilate the culture chamber, so as to facilitate the adjustment of temperature. The humidification mechanism is used to adjust the humidity in the culture chamber. The temperature and humidity measuring mechanism is used for the temperature and humidity in the culture chamber. The first lighting lamp is used for providing illumination; The pesticide is diluted according to requirements, and the diluted pesticide is poured into the medicine adding funnel. The pesticide enters the pesticide application mechanism through the medicine adding funnel. The air pressure tank is used to pass pressurized gas into the pesticide application mechanism, so that the pesticide is sprayed out from the medicine spraying nozzle. The utility model has a culture chamber and multiple bioassay chambers. In the culture chamber, multiple culture dishes can be cultured simultaneously, so that the culture conditions of multiple groups of Psylla chinensis can be guaranteed to be the same, and the influence on Psylla chinensis caused by different culture conditions can be reduced. Multiple culture dishes can enter the bioassay chamber simultaneously, so that Psylla chinensis can conduct experiments under the same conditions. Different concentrations of pesticides can be added into multiple pesticide application mechanisms respectively, which is convenient for multiple groups of Psylla chinensis to conduct experiments simultaneously, reducing the steps in the experimental process and reducing the systematic error caused by mutual waiting between different treatments. Description of the Drawings
[0019] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required to be used in the embodiments. Obviously, the following described drawings are only some embodiments of the present utility model. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.
[0020] Figure 1 This is a schematic structural diagram of the device for detecting the biological activity of pear psyllids against chemical pesticides in the present utility model;
[0021] Figure 2 It is Figure 1 a partial enlarged schematic view of a in
[0022] Figure 3 It is Figure 1 a partial enlarged schematic view of b in
[0023] Figure 4 It is Figure 1 a partial enlarged schematic view of c in
[0024] Figure 5 It is Figure 1 a partial enlarged schematic view of d in
[0025] Figure 6 It is Figure 1 a side cross-sectional view of the present utility model in
[0026] Among them, 1. Box body; 2. Culture chamber; 3. Bioassay chamber; 4. Petri dish; 5. Electric telescopic rod; 6. Bottom plate; 7. First through hole; 8. First lighting lamp; 9. Drug spray head; 10. Medicine adding funnel; 11. Pressure tank; 12. Controller; 13. Horizontal plate; 14. Vertical plate; 15. Lower retaining ring; 16. Upper retaining ring; 17. Sealing ring; 18. Groove; 19. Medicine cylinder; 20. First connecting pipe; 21. Manual valve; 22. Second connecting pipe; 23. Solenoid valve; 24. Third connecting pipe; 25. Temperature and humidity sensor; 26. Hot air blower; 27. Blower; 28. Ventilation pipe; 29. Air outlet; 30. Water mist spray head; 31. Water pipe; 32. Water pump; 33. Second through hole; 34. Cover; 35. Sleeve; 36. Rotating shaft; 37. Magnet one; 38. Magnet two; 39. Fixed seat; 40. Second lighting lamp; 41. Taking port one; 42. Taking port two; 43. Sealing door one; 44. Sealing door two. Specific embodiments
[0027] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative work shall fall within the protection scope of the present utility model.
[0028] To make the above objects, features, and advantages of the present utility model more obvious and understandable, the present utility model will be further described in detail below in conjunction with the drawings and specific embodiments.
[0029] Reference Figure 1-6 , the present utility model provides a device for detecting the biological activity of pear psyllids against chemical pesticides, including:
[0030] A box body 1, in which a culture chamber 2 and several bioassay chambers 3 are arranged,
[0031] A culture component, which includes a culture dish 4. Several electric telescopic rods 5 are fixedly connected in the culture chamber 2. The output end of the electric telescopic rod 5 is fixedly connected with a bottom plate 6. The culture dish 4 is arranged on the bottom plate 6. A first through hole 7 is arranged between the bioassay chamber 3 and the culture chamber 2. The bottom plate 6 is arranged corresponding to the first through hole 7. A ventilation mechanism, a humidification mechanism and a temperature and humidity measurement mechanism are arranged in the culture chamber 2. A first lighting lamp 8 is fixedly connected in the culture chamber 2;
[0032] A bioassay component, which includes several dosing mechanisms. The dosing mechanisms are arranged on the bioassay chamber 3. The dosing mechanisms are provided with medicine spray nozzles 9 and medicine adding funnels 10. The dosing mechanisms are communicated with a pressure tank 11, and the pressure tank 11 is communicated with a gas source;
[0033] A controller 12, which is fixedly connected to the outer side wall of the box body 1. The electric telescopic rod 5, the ventilation mechanism, the humidification mechanism, the temperature and humidity measurement mechanism, the first lighting lamp 8 and the dosing mechanism are all electrically connected to the controller 12.
[0034] The culture chamber 2 is used for culturing pear psyllids. The bioassay chamber 3 is used for detecting the effect of chemical pesticides on pear psyllids. The culture dish 4 is used for placing pear psyllids. In practical applications, pear psyllids are placed on pear tree leaves, and the pear tree leaves are placed in the culture dish 4. The electric telescopic rod 5 is used to raise or lower the culture dish 4, so that the culture dish 4 enters the bioassay chamber 3 from the culture chamber 2. After the electric telescopic rod 5 drives the bottom plate 6 to rise, the bottom plate 6 will enter the first through hole 7, so that the bioassay chamber 3 becomes a relatively airtight space. The ventilation mechanism is used to ventilate the culture chamber 2, so as to facilitate temperature adjustment. The humidification mechanism is used to adjust the humidity in the culture chamber 2. The temperature and humidity measurement mechanism is used for the temperature and humidity in the culture chamber 2. The first lighting lamp 8 is used to provide illumination; The pesticide is diluted according to requirements, and the diluted pesticide is poured into the medicine adding funnel 10. The pesticide enters the dosing mechanism through the medicine adding funnel 10. The pressure tank 11 is used to pass pressurized gas into the dosing mechanism, so that the pesticide is sprayed out from the medicine spray nozzle 9. This device has one culture chamber 2 and multiple bioassay chambers 3. In the culture chamber 2, multiple culture dishes 4 can be cultured simultaneously, so as to ensure that the culture conditions of multiple groups of pear psyllids are the same, reduce the influence on pear psyllids caused by different culture conditions. Multiple culture dishes 4 can enter the bioassay chamber 3 simultaneously, so that pear psyllids can conduct experiments under the same conditions. Different concentrations of pesticides can be added into multiple dosing mechanisms respectively, which is convenient for multiple groups of pear psyllids to conduct experiments simultaneously, reduces the steps in the experimental process, and reduces the systematic error caused by waiting between different treatments.
[0035] For a further optimized solution, a horizontal plate 13 and a number of vertical plates 14 are fixedly connected inside the box body 1. The vertical plates 14 are fixedly connected to the horizontal plate 13. The horizontal plate 13 and the vertical plates 14 divide the inside of the box body 1 into a culture chamber 2 and a bioassay chamber 3. The first through hole 7 is opened on the horizontal plate 13.
[0036] The horizontal plate 13 and the vertical plates 14 divide the internal space of the box body 1 into one culture chamber 2 and multiple bioassay chambers 3. One culture chamber 2 facilitates the cultivation of multiple groups of Psylla chinensis, reducing the differences caused by cultivation conditions among different groups of Psylla chinensis. Multiple bioassay chambers 3 facilitate the simultaneous experimentation on multiple groups of Psylla chinensis.
[0037] For a further optimized solution, a lower retaining ring 15 is fixedly connected to the bottom plate 6, and an upper retaining ring 16 is fixedly connected inside the first through hole 7. The lower retaining ring 15 and the upper retaining ring 16 are arranged correspondingly. A sealing ring 17 is fixedly connected to the lower retaining ring 15, and a groove 18 is opened on the upper retaining ring 16. The sealing ring 17 and the groove 18 are arranged correspondingly.
[0038] After the bottom plate 6 rises, the lower retaining ring 15 contacts the upper retaining ring 16, and at the same time, the sealing ring 17 enters the groove 18, thereby improving the sealing performance inside the bioassay chamber 3.
[0039] For a further optimized solution, the drug application mechanism includes a medicine cylinder 19. The medicine cylinder 19 is arranged inside the culture chamber 2. The medicine cylinder 19 extends out of the box body 1. A first connecting pipe 20 is communicated with the medicine cylinder 19. A manual valve 21 is installed on the first connecting pipe 20. A medicine adding funnel 10 is fixedly connected to the first connecting pipe 20. A second connecting pipe 22 is communicated with the medicine cylinder 19. One end of the second connecting pipe 22 far from the medicine cylinder 19 is communicated with the air pressure tank 11. The air pressure tank 11 is communicated with a gas source. An electromagnetic valve 23 is installed on the second connecting pipe 22. A third connecting pipe 24 is communicated with the medicine cylinder 19. A drug spray head 9 is fixedly connected to the third connecting pipe 24. The electromagnetic valve 23 is electrically connected to the controller 12.
[0040] The medicine adding funnel 10 is outside the bioassay chamber 3, facilitating the addition of pesticides. First, the manual valve 21 is opened, and the pesticide enters the first connecting pipe 20 through the medicine adding funnel 10 and then enters the medicine cylinder 19 from the first connecting pipe 20. After the liquid medicine enters the medicine cylinder 19, the manual valve 21 is closed. The medicine cylinder 19 can store a relatively large amount of liquid medicine. The electromagnetic valve 23 is opened or closed under the control of the controller 12. The air pressure tank 11 is filled with high-pressure gas. The high-pressure gas enters the medicine cylinder 19 through the second connecting pipe 22. The liquid medicine in the medicine cylinder 19 will not exceed the position of the second connecting pipe 22. The third connecting pipe 24 is thinner than the medicine cylinder 19, so that the liquid medicine can have a higher pressure when it enters the drug spray head 9 from the medicine cylinder 19. The drug spray head 9 can spray the liquid medicine in a mist form.
[0041] For a further optimized solution, the temperature and humidity measuring mechanism includes a number of temperature and humidity sensors 25, which are fixedly connected inside the culture chamber 2, and the temperature and humidity sensors 25 are electrically connected to the controller 12.
[0042] The temperature and humidity sensors 25 are used to measure the temperature and humidity inside the culture chamber 2 and the biological testing chamber 3, and can transmit the temperature and humidity to the controller 12.
[0043] For a further optimized solution, the ventilation mechanism includes a hot air blower 26, a blower 27 and a ventilation pipe 28. The ventilation pipe 28 extends into the culture chamber 2, and a number of air outlets 29 are provided on the ventilation pipe 28. The hot air blower 26 and the blower 27 are both communicated with the ventilation pipe 28, and the hot air blower 26 and the blower 27 are both electrically connected to the controller 12.
[0044] The hot air blower 26 is used to generate hot air, and the blower 27 can introduce natural air into the culture chamber 2. After the temperature is measured by the temperature and humidity sensors 25, the controller 12 will control the hot air blower 26 or the blower 27 to work according to the temperature value, and send hot air or natural air into the culture chamber 2.
[0045] For a further optimized solution, the humidifying mechanism includes a number of water mist nozzles 30, which are installed on the water pipe 31. The water mist nozzles 30 are communicated with the water pipe 31. The water mist nozzles 30 are located inside the culture chamber 2. The water pipe 31 extends out of the box body 1, and the water pipe 31 is communicated with a water pump 32. The water inlet end of the water pump 32 is communicated with a water source.
[0046] The water pump 32 pumps water through the water pipe 31 to the water mist nozzles 30, and the water is sprayed out in a mist shape through the water mist nozzles 30. The water pump 32 can be started through the controller 12 according to the humidity condition inside the culture chamber 2.
[0047] For a further optimized solution, a second through hole 33 is provided on the side wall of the box body 1. The second through hole 33 is located inside the culture chamber 2. A baffle 34 is provided on the second through hole 33. The baffle 34 is rotatably connected to the side wall of the box body 1. A sleeve 35 is fixedly connected to the side wall of the box body 1. A rotating shaft 36 is rotatably connected inside the sleeve 35. The baffle 34 is fixedly connected to the rotating shaft 36. A magnet one 37 is fixedly connected to the baffle 34, and a magnet two 38 is fixedly connected to the side wall of the box body 1. The magnet one 37 and the magnet two 38 are arranged correspondingly.
[0048] The second through hole 33 facilitates the discharge of the gas inside the culture chamber 2. The baffle 34 is used to block the second through hole 33. When the hot air blower 26 and the blower 27 do not introduce gas into the culture chamber 2, the baffle 34 will block the second through hole 33 to prevent the internal gas from flowing out. When gas is introduced into the culture chamber 2, the baffle 34 will be opened under the action of air pressure. The magnetic attraction force between the magnet one 37 and the magnet two 38 is not large, and the acting force of the air pressure can separate them.
[0049] For a further optimized solution, a fixing base 39 is fixedly connected to the bottom plate 6, the culture dish 4 is snap-fitted into the fixing base 39, several second lighting lamps 40 are fixedly connected in the bioassay chamber 3, the second lighting lamps 40 are electrically connected to the controller 12, a first access opening 41 is formed in the bioassay chamber 3, a second access opening 42 is formed in the culture chamber 2, a sealing door 43 is rotatably connected to the first access opening 41, and a sealing door 44 is rotatably connected to the second access opening 42.
[0050] The fixing base 39 is used to fix the culture dish 4. The second lighting lamps 40 facilitate observing the situation inside the bioassay chamber 3. Each bioassay chamber 3 is provided with a first access opening 41 for conveniently taking out the culture dish 4 after spraying pesticides to observe the pear psyllids. The second access opening 42 on the culture chamber 2 is relatively large for conveniently taking out multiple culture dishes 4. The sealing door 43 is used to seal the first access opening 41, and the sealing door 44 is used to seal the second access opening 42. Both the sealing door 43 and the sealing door 44 adopt existing technologies. In this embodiment, the box body 1, the sealing door 43, and the sealing door 44 are all made of transparent materials for conveniently observing the internal situation.
[0051] A method for detecting the biological activity of chemical pesticides against pear psyllids includes the following steps:
[0052] Step 1: Obtain initial nymphs. Before the early spring buds sprout, directly collect the winter-type adult pear psyllids from the field, make an insect rearing cage with a 100-mesh gauze, and isolate and rear them on potted pear seedlings. To accelerate the test process, the potted seedlings can be placed in a solar greenhouse or an artificial climate chamber for short-term rearing.
[0053] Step 2: Collect flat pear tree leaves of the same variety as their previous generation in the field. Remove other insects and eggs on them under a dissecting microscope, place the leaves face down on the gauze, and make the four sides of the leaves fit as closely as possible with the gauze. Then, wrap the edges of the leaves with a thin strip made of absorbent cotton or blotting paper, etc., so as to keep the leaves fresh by absorbing water, and at the same time prevent the pear psyllids from escaping. Wrap the base of the petiole with absorbent cotton and connect it to the water under the isolation table for moisturizing the leaves. Move suitable-sized nymphs onto the pear tree leaves for standby. Prepare multiple pear tree leaves, and the number of nymphs on each pear tree leaf is the same.
[0054] Step 3: Put multiple pear tree leaves with nymphs into different culture dishes 4 respectively, place the culture dishes 4 into the fixing seats 39 on the bottom plate 6. The temperature and humidity sensor 25 measures the temperature and humidity in the culture chamber 2, and transmits the measured values to the controller 12. According to the measurement results, the controller 12 starts the hot air blower 26 or the air blower 27 to adjust the temperature in the culture chamber 2, and starts the water pump 32. The water pump 32 pumps water through the water pipe 31 to the water mist nozzle 30, and the water is sprayed out in a mist through the water mist nozzle 30 to adjust the humidity in the culture chamber 2. The temperature is set at 25±1°C, the relative humidity is 70%±5%, and the light cycle is 16L:8D for cultivation. After all preparations are completed, the bioassay experiment can be started;
[0055] Step 4: After the cultivation is completed, the controller 12 starts the electric telescopic rod 5 to send multiple culture dishes 4 to different bioassay chambers 3 at the same time. After the bottom plate 6 rises, the lower retaining ring 15 contacts the upper retaining ring 16, and at the same time, the sealing ring 17 enters the groove 18, thereby improving the sealing performance in the bioassay chamber 3. Set one of the culture dishes 4 as the control group, and spray pesticides on the other culture dishes 4. First, dilute the pesticides, and then add the diluted pesticides to the dosing funnel 10. The pesticides enter the first connecting pipe 20 through the dosing funnel 10, and then enter the medicine cylinder 19 from the first connecting pipe 20. The air pressure tank 11 is filled with high-pressure gas, and the high-pressure gas enters the medicine cylinder 19 through the second connecting pipe 22. After the liquid medicine enters the medicine cylinder 19, close the manual valve 21, and the solenoid valve 23 is opened under the control of the controller 12. The high-pressure gas is introduced into the medicine cylinder 19 through the air pressure tank 11, and the pressurized gas makes the pesticides spray out from the medicine nozzle 9. The pesticide addition amount is 1 ml, the gas pressure introduced by the pesticide application mechanism is 10 psi (0.07 MPa), and the sedimentation time is 30 seconds;
[0056] Step 5: Open the first sealing door 43, take out all the culture dishes 4, observe the survival situation of the pear psylla nymphs, and compare with the control group.
[0057] In the description of the present invention, it should be understood that the orientation or positional relationship indicated by the terms "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be construed as a limitation of the present invention.
[0058] The embodiments described above are only descriptions of the preferred embodiments of the present invention, and do not limit the scope of the present invention. Without departing from the design spirit of the present invention, various deformations and improvements made by those of ordinary skill in the art to the technical solutions of the present invention shall fall within the protection scope determined by the claims of the present invention.
Claims
1. An apparatus for detecting the biological activity of pear psyllids against chemical pesticides, characterized in that , including: A box body (1), a culture chamber (2) and a number of bioassay chambers (3) are arranged inside the box body (1), A culture component, the culture component includes a culture dish (4), a number of electric telescopic rods (5) are fixedly connected inside the culture chamber (2), the output end of the electric telescopic rod (5) is fixedly connected with a bottom plate (6), the culture dish (4) is arranged on the bottom plate (6), a first through hole (7) is arranged between the bioassay chamber (3) and the culture chamber (2), the bottom plate (6) is correspondingly arranged with the first through hole (7), a ventilation mechanism, a humidification mechanism and a temperature and humidity measuring mechanism are arranged inside the culture chamber (2), and a first lighting lamp (8) is fixedly connected inside the culture chamber (2); A bioassay component, the bioassay component includes a number of dosing mechanisms, the dosing mechanisms are arranged on the bioassay chamber (3), the dosing mechanisms are provided with medicine spray nozzles (9) and medicine adding funnels (10), the dosing mechanisms are communicated with a pressure tank (11), and the pressure tank (11) is communicated with a gas source; A controller (12), the controller (12) is fixedly connected to the outer side wall of the box body (1), and the electric telescopic rod (5), the ventilation mechanism, the humidification mechanism, the temperature and humidity measuring mechanism, the first lighting lamp (8) and the dosing mechanism are all electrically connected to the controller (12).
2. The device for detecting the biological activity of Psylla pyrisuga to chemical pesticides according to claim 1, wherein: A transverse plate (13) and a number of vertical plates (14) are fixedly connected inside the box body (1), the vertical plates (14) are fixedly connected to the transverse plate (13), the transverse plate (13) and the vertical plates (14) divide the inside of the box body (1) into the culture chamber (2) and the bioassay chamber (3), and the first through hole (7) is opened on the transverse plate (13).
3. The device for detecting the biological activity of Psylla pyrisuga Forster to chemical pesticides according to claim 1, wherein: A lower retaining ring (15) is fixedly connected to the bottom plate (6), an upper retaining ring (16) is fixedly connected inside the first through hole (7), the lower retaining ring (15) is correspondingly arranged with the upper retaining ring (16), a sealing ring (17) is fixedly connected to the lower retaining ring (15), a groove (18) is opened on the upper retaining ring (16), and the sealing ring (17) is correspondingly arranged with the groove (18).
4. The device for detecting the bioactivity of pear psyllids to chemical pesticides according to claim 1, characterized in that: The dosing mechanism includes a medicine cylinder (19), the medicine cylinder (19) is arranged inside the culture chamber (2), the medicine cylinder (19) extends out of the box body (1), a first connecting pipe (20) is communicated with the medicine cylinder (19), a manual valve (21) is installed on the first connecting pipe (20), the medicine adding funnel (10) is fixedly connected to the first connecting pipe (20), a second connecting pipe (22) is communicated with the medicine cylinder (19), one end of the second connecting pipe (22) far away from the medicine cylinder (19) is communicated with the pressure tank (11), the pressure tank (11) is communicated with a gas source, a solenoid valve (23) is installed on the second connecting pipe (22), a third connecting pipe (24) is communicated with the medicine cylinder (19), the medicine spray nozzle (9) is fixedly connected to the third connecting pipe (24), and the solenoid valve (23) is electrically connected to the controller (12).
5. The device for detecting the bioactivity of pear psyllids against chemical pesticides according to claim 1, characterized in that: The temperature and humidity measuring mechanism includes a plurality of temperature and humidity sensors (25), and the plurality of temperature and humidity sensors (25) are fixedly connected inside the culture chamber (2). The temperature and humidity sensors (25) are electrically connected to the controller (12).
6. The device for detecting the bioactivity of pear psyllids to chemical pesticides according to claim 1, characterized in that: The ventilation mechanism includes a hot air blower (26), a blower (27) and a ventilation pipe (28). The ventilation pipe (28) extends into the culture chamber (2). A plurality of air outlets (29) are formed in the ventilation pipe (28). Both the hot air blower (26) and the blower (27) are communicated with the ventilation pipe (28), and both the hot air blower (26) and the blower (27) are electrically connected to the controller (12).
7. An apparatus for detecting the bioactivity of pear psyllids against chemical pesticides according to claim 1, characterized in that: The humidifying mechanism includes a plurality of water mist nozzles (30). The water mist nozzles (30) are installed on a water pipe (31). The water mist nozzles (30) are communicated with the water pipe (31). The water mist nozzles (30) are located inside the culture chamber (2). The water pipe (31) extends out of the box body (1). The water pipe (31) is communicated with a water pump (32), and the water inlet end of the water pump (32) is communicated with a water source.
8. The device for detecting the bioactivity of pear psyllids against chemical pesticides according to claim 1, characterized in that: A second through hole (33) is formed in the side wall of the box body (1). The second through hole (33) is located inside the culture chamber (2). A baffle (34) is arranged on the second through hole (33). The baffle (34) is rotatably connected to the side wall of the box body (1). A sleeve (35) is fixedly connected to the side wall of the box body (1). A rotating shaft (36) is rotatably connected inside the sleeve (35). The baffle (34) is fixedly connected to the rotating shaft (36). A first magnet (37) is fixedly connected to the baffle (34). A second magnet (38) is fixedly connected to the side wall of the box body (1). The first magnet (37) and the second magnet (38) are arranged corresponding to each other.
9. An apparatus for detecting the bioactivity of chemical pesticides against Psylla pyrisuga, characterized in that: A fixed seat (39) is fixedly connected to the bottom plate (6). The culture dish (4) is clamped inside the fixed seat (39). A plurality of second lighting lamps (40) are fixedly connected inside the bioassay chamber (3). The second lighting lamps (40) are electrically connected to the controller (12). A first access opening (41) is formed in the bioassay chamber (3). A second access opening (42) is formed in the culture chamber (2). A sealing door one (43) is rotatably connected to the first access opening (41). A sealing door two (44) is rotatably connected to the second access opening (42).