Experimental device suitable for in-vitro blade

Abstract

The experimental device comprises a culture dish cover, a base plate and a petiole sleeve, a through hole is formed in the upper portion of the culture dish cover, a gauze element is connected to the upper portion of the through hole, an upper groove is formed in the side wall of the culture dish cover, an upper petiole pipe is connected to the upper groove, a lower groove is formed in the side wall of the base plate, and a lower petiole pipe is connected to the lower groove. The blade handle is sleeved on the side edge of the chassis. The experimental device has the advantages that the experimental device is easy to manufacture, materials can be obtained in a laboratory, mass manufacturing is facilitated, and cost is low; the activity or counting of the netbug is easy to observe in an experiment, the preservation effect of the platanus leaves is good, and the leaves do not need to be frequently replaced so as to reduce disturbance to the netbug; and the petiole is fixed in the petiole sleeve by using the absorbent cotton which is fully dipped with water, so that the water can be supplemented to the leaves at any time under the condition that the culture dish is not opened.

Description

Technical Field

[0001] The utility model relates to an experimental device applicable to detached leaves, belonging to the technical field of biological control research of forestry pests. Background Art

[0002] The activity ability of Corythucha ciliata is weak, and the entire life cycle of some individuals is completed on one leaf. However, Corythucha ciliata is small in size, and the leaves of its host plane tree are relatively large, making it difficult to preserve the detached leaves fresh. Improper experimental operation can easily lead to inaccuracy of the experiment. During the experiment, on the one hand, it is necessary to ensure the freshness of the host plant to ensure that the insects can feed normally. On the other hand, it is necessary to prevent the insects from escaping. Finally, it is also necessary to meet the requirement of facilitating the observation of their biological characteristics. This requires a device that meets the above three requirements.

[0003] Existing biological experiments related to lace bugs mostly use rectangular plastic boxes or water-inserted branches in the laboratory. Although the space in the plastic box is relatively large, it is very easy for lace bugs to escape, and it is not convenient for observation. There are also certain problems with the freshness preservation of leaves. At the same time, the air permeability of the plastic box is poor, and the water transpired by the leaves adheres to the inner wall of the plastic box, which is easy to cause the drowning of lace bugs. In the water-inserted branch experiment, although the leaf state in the natural state is simulated to a certain extent, it is very easy to disturb the leaves during the experiment, affecting the normal life activities of lace bugs and the accuracy of the experiment. Due to the large size of plane tree leaves and strong transpiration, the method of water-inserted branches is easy to make the freshness preservation of leaves not in place, affecting the quality of food for lace bugs. This method is also easy to cause the escape of lace bugs or their hiding in the surrounding containers and being difficult to observe. Therefore, an experimental device that is not only easy to observe, the leaves are easy to preserve fresh, effectively prevents the escape of lace bugs, and causes little disturbance to lace bugs during the experiment and is easy to manufacture is needed. Content of the Utility Model

[0004] The technical problem to be solved by the utility model is to provide an experimental device applicable to detached leaves, which is easy to observe, the leaves are easy to preserve fresh, effectively prevents the escape of lace bugs, and causes little disturbance to lace bugs during the experiment.

[0005] The utility model is realized by the following scheme: an experimental device applicable to detached leaves, which includes a culture dish cover, a chassis and a petiole sleeve. A through hole is opened in the upper part of the culture dish cover, and a gauze is connected above the through hole. An upper groove is opened on the side wall of the culture dish cover, and an upper petiole tube is connected at the upper groove. A lower groove is opened on the side wall of the chassis, and a lower petiole tube is connected at the lower groove. The petiole sleeve is on the side of the chassis.

[0006] The culture dish cover is a cylindrical shape with a lower opening, and the chassis is a cylindrical shape with an upper opening. The culture dish cover is sleeved on the chassis.

[0007] The culture dish cover and the chassis are made of transparent PC board, with a diameter of 150 mm.

[0008] After the upper petiole tube and the lower petiole tube are closed, they are in a cylindrical shape.

[0009] The outer ring of the screen is connected to an adhesive edge, and the adhesive edge is connected to the culture dish cover.

[0010] The volume of the petiole sleeve is 10 ml.

[0011] The side walls of the culture dish cover and the chassis are connected through a sealing part.

[0012] The sealing part is a rubber sealing layer, and the inner wall of the culture dish cover is connected to the rubber sealing layer.

[0013] The rubber sealing layer has two circles.

[0014] The beneficial effects of the present utility model are as follows:

[0015] 1. The experimental device suitable for use on excised leaves of the present utility model is simple to manufacture, and the materials can all be obtained in the laboratory, facilitating mass production and having a low cost;

[0016] 2. The experimental device suitable for use on excised leaves of the present utility model is easy to observe the activities or count of lace bugs during the experiment, has a good fresh-keeping effect on the leaves of Platanus acerifolia, and there is no need to frequently replace the leaves to reduce the disturbance to lace bugs;

[0017] 3. The experimental device suitable for use on excised leaves of the present utility model can conveniently supply water to the leaves. By fixing the petiole with absorbent cotton dipped in water in the petiole sleeve, water can be supplied to the leaves at any time without opening the culture dish. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a three-dimensional exploded structure schematic diagram of an experimental device suitable for use on excised leaves of the present utility model.

[0019] Figure 2 It is a three-dimensional structure schematic diagram of an experimental device suitable for use on excised leaves of the present utility model.

[0020] Figure 3 It is a three-dimensional structure schematic diagram of an experimental device suitable for use on excised leaves of the present utility model in the usage state.

[0021] Figure 4 It is a front view sectional structure schematic diagram of an experimental device suitable for use on excised leaves of the present utility model.

[0022] Figure 5 It is an experimental device suitable for use on excised leaves of the present utility modelFigure 4 Schematic diagram of the large sample structure of A

[0023] In the figure: 100 is the culture dish lid, 101 is the through hole, 102 is the screen, 103 is the upper groove, 104 is the upper petiole tube, 105 is the bonding edge, 200 is the chassis, 201 is the lower groove, 202 is the lower petiole tube, 300 is the petiole sleeve, 400 is the sealing part, 401 is the rubber sealing layer, 500 is the Platanus acerifolia leaf, and 600 is the Platanus acerifolia petiole. Specific implementation mode

[0024] The following is combined with Figures 1-5 to further illustrate the present utility model, but the protection scope of the present utility model is not limited to the described content.

[0025] Embodiment 1

[0026] Referring to Figure 1 and Figure 2 , an experimental device suitable for use on excised leaves, which includes a culture dish lid 100, a chassis 200, and a petiole sleeve 300. A through hole 101 is opened in the upper part of the culture dish lid 100, a screen 102 is connected to the upper part of the through hole 101, an upper groove 103 is opened in the side wall of the culture dish lid 100, an upper petiole tube 104 is connected at the upper groove 103, a lower groove 201 is opened in the side wall of the chassis 200, a lower petiole tube 202 is connected at the lower groove 201, and the petiole sleeve 300 is on the side of the chassis 200.

[0027] The culture dish lid 100 is a cylindrical shape with a lower opening, the chassis 200 is a cylindrical shape with an upper opening, the culture dish lid 100 is sleeved on the chassis 200, the side walls of the culture dish lid 100 and the chassis 200 are connected through a sealing part 400, the culture dish lid 100 and the chassis 200 are made of transparent PC board material, with a diameter of 150 mm, and the volume of the petiole sleeve 300 is 10 ml.

[0028] After the upper petiole tube 104 and the lower petiole tube 202 are closed, they are in a cylindrical shape.

[0029] For the usage state, referring to Figure 3 , place the Platanus acerifolia leaf 500 in the chassis 200, then cover the culture dish lid 100 on the chassis 200. At this time, the upper petiole tube 104 and the lower petiole tube 202 are closed to form a circular tube. Pass the Platanus acerifolia petiole 600 through the circular tube formed by the closed upper petiole tube 104 and lower petiole tube 202, and extend the end of the Platanus acerifolia petiole 600 into the petiole sleeve 300. Fix the Platanus acerifolia petiole 600 with absorbent cotton dipped in water in the petiole sleeve 300. It is possible to supplement water to the Platanus acerifolia leaf 500 without opening the culture dish. In this way, the Platanus acerifolia leaf 500 can be well preserved, and at the same time, it is convenient for the experimenter to observe.

[0030] After the plane tree petiole 600 passes through the circular tube formed by the closure of the upper petiole tube 104 and the lower petiole tube 202, there is still a large gap between the upper petiole tube 104 and the lower petiole tube 202, which can be sealed by stuffing absorbent cotton.

[0031] Example 2

[0032] The outer ring of the screen 102 is connected to the bonding edge 105, and the bonding edge 105 is provided with an adhesive. The bonding edge 105 is fixed to the culture dish cover 100 through the adhesive.

[0033] Example 3

[0034] Reference Figure 4 And Figure 5 In reference to

[0035] Although the technical solutions of the present invention have been described in detail and enumerated, it should be understood that for those skilled in the art, making modifications to the above embodiments or adopting equivalent alternative solutions are obvious to those skilled in the art. These modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection required by the present invention.

Claims

1. An experimental device applicable to in vitro leaves, characterized in that It includes a culture dish lid (100), a chassis (200) and a petiole sleeve (300). A through hole (101) is provided in the upper part of the culture dish lid (100), a screen (102) is connected to the upper part of the through hole (101), an upper groove (103) is provided in the side wall of the culture dish lid (100), an upper petiole tube (104) is connected at the upper groove (103), a lower groove (201) is provided in the side wall of the chassis (200), a lower petiole tube (202) is connected at the lower groove (201), and the petiole sleeve (300) is on the side of the chassis (200).

2. An experimental device applicable to in vitro leaves according to claim 1, characterized in that, The culture dish lid (100) is a cylindrical shape with a lower opening, the chassis (200) is a cylindrical shape with an upper opening, and the culture dish lid (100) is sleeved on the chassis (200).

3. An experimental device applicable to in vitro leaves according to claim 1, characterized in that, The culture dish lid (100) and the chassis (200) are made of transparent PC board material and have a diameter of 150 mm.

4. An experimental device applicable to in vitro leaves according to claim 1, characterized in that, The upper petiole tube (104) and the lower petiole tube (202) are cylindrical in shape when closed.

5. An experimental device applicable to in vitro leaves according to claim 1, characterized in that, An outer ring of the screen (102) is connected to an adhesive edge (105), and the adhesive edge (105) is connected to the culture dish lid (100).

6. An experimental device applicable to in vitro leaves according to claim 1, characterized in that, The volume of the petiole sleeve (300) is 10 ml.

7. An experimental device applicable to in vitro leaves according to claim 1, characterized in that, The side walls of the culture dish lid (100) and the chassis (200) are connected through a sealing part (400).

8. An experimental device applicable to an in vitro leaf according to claim 7, characterized in that, The sealing part (400) is a rubber sealing layer (401), and the inner wall of the culture dish lid (100) is connected to the rubber sealing layer (401).

9. An experimental device applicable to in vitro leaves according to claim 8, characterized in that, The rubber sealing layer (401) has two circles.

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