Nucleic acid extraction device capable of preventing cross contamination

Abstract

The utility model belongs to the technical field of nucleic acid extraction equipment, and particularly relates to a nucleic acid extraction device capable of preventing cross contamination. The extraction cavities are all arranged on the base, the first driving mechanism is arranged on the base, and the output end of the first driving mechanism penetrates through the extraction cavities to be connected with the extraction carrying frame; an extraction tube with a magnetic bar sleeve, an extraction tube with a washing liquid and an extraction tube with an eluent are sequentially distributed on the extraction carrying frame in the front-back direction; the second driving mechanism is arranged at the upper end of the extraction cavity; the stirring rod is connected with the magnetic rod sleeve; the third driving mechanism is arranged at the upper end of the extraction cavity. The first driving mechanism is used for driving the extraction carrying frame to correspond to the stirring rods, the second driving mechanism is used for driving the stirring rods to extend into the extraction pipe with the magnetic rod sleeve, the extraction pipe with the washing liquid and the extraction pipe with the eluent respectively, and the third driving mechanism is used for driving the stirring rods to rotate, so that the pollution-free nucleic acid extract can be obtained; therefore, the accuracy of nucleic acid detection results is ensured.

Description

Technical Field

[0001] The utility model belongs to the technical field of nucleic acid extraction equipment, and particularly relates to a nucleic acid extraction device for preventing cross-contamination. Background Art

[0002] The primary key of nucleic acid detection technology is to extract nucleic acid from biological samples. Therefore, effectively and accurately extracting nucleic acid templates becomes a prerequisite for subsequent nucleic acid detection. Nucleic acid extraction is to extract effective nucleic acid using microorganisms, animals and plants, human body fluids or tissues as samples.

[0003] However, cross-contamination caused by the mutual influence of aerosol and liquid droplets between different samples easily affects the accuracy of nucleic acid detection results. Therefore, it is necessary to design a nucleic acid extraction device for preventing cross-contamination to solve the above problems. Summary of the Utility Model

[0004] In view of the above problems, the utility model provides a nucleic acid extraction device for preventing cross-contamination to solve the problems raised in the above background art.

[0005] To achieve the above object, the utility model provides the following technical solution: A nucleic acid extraction device for preventing cross-contamination, comprising a base, an extraction chamber, an extraction carrier, a first driving mechanism, a second driving mechanism and a third driving mechanism. The extraction chamber is arranged on the base. The extraction carrier is used to be arranged in the extraction chamber. The first driving mechanism is arranged on the base, and the output end of the first driving mechanism passes through the extraction chamber and is connected to the extraction carrier. The first driving mechanism is used to drive the extraction carrier to move in the front-back direction;

[0006] Extraction tubes with magnetic rod sleeves, extraction tubes with washing solution and extraction tubes with eluent are sequentially distributed on the extraction carrier in the front-back direction;

[0007] The second driving mechanism is arranged at the upper end of the extraction chamber, and the output end of the second driving mechanism is used to be connected to a stirring rod, and the stirring rod is used to be connected to the magnetic rod sleeve;

[0008] The third driving mechanism is arranged at the upper end of the extraction chamber, and the third driving mechanism is used to drive the stirring rod to stir.

[0009] Further, the nucleic acid extraction device for preventing cross - contamination further includes a rear side plate, which is connected to the rear side of the extraction chamber. The first driving mechanism includes a motor, a lead screw, a lead screw nut, a sliding seat, and a conduit. The motor is arranged on the base, the axial direction of the lead screw is in the front - rear direction, one end of the lead screw is connected to the output shaft of the motor, and the other end of the lead screw is used to be connected to the rear side plate. The lead screw nut is sleeved on the lead screw and connected to the sliding seat. One end of the conduit is connected to the sliding seat, and the other end of the conduit passes through the extraction chamber and is connected to the extraction carrier.

[0010] Further, the first driving mechanism further includes a bearing seat and a linear bearing. The bearing seat is connected to the rear side plate, and the linear bearing is arranged inside the bearing seat. The conduit sequentially passes through the linear bearing and the bearing seat.

[0011] Further, the nucleic acid extraction device for preventing cross - contamination further includes an extraction chamber door and a door slide bar. The extraction chamber door is located on the front side of the extraction chamber. The rear side of the door slide bar is slidably connected to the conduit, and the front side of the door slide bar is used to be connected to the extraction chamber door;

[0012] The first driving mechanism further includes a spring. The spring is arranged inside the conduit and is coaxially distributed with the conduit. One end of the spring is connected to the conduit, and the other end of the spring is connected to the door slide bar.

[0013] Further, the extraction carrier includes a base, a heat insulation plate, a heating film, a thermal conductive silicone grease, a tube seat, and a heating seat. The base is connected to the conduit. The heat insulation plate is arranged on the upper end of the base. Heating seats are arranged on both the front and rear sides of the upper end of the heat insulation plate. The tube seat is arranged in the middle of the upper end of the heat insulation plate. The heating film and the thermal conductive silicone grease are sequentially arranged between the heat insulation plate and the heating seats from bottom to top. The front heating seat is used to arrange an extraction tube with a magnetic rod sleeve. The tube seat is used to arrange an extraction tube with washing solution. The rear heating seat is used to arrange an extraction tube with elution solution.

[0014] Further, the second driving mechanism includes a column, a first bottom plate, a first lead screw, a first lead screw nut, a first nut seat, a second bottom plate, and a first linear motor;

[0015] The first bottom plate is connected to the base through the column. The first linear motor is arranged on the first bottom plate. The first nut seat is connected to the second bottom plate. The first lead screw nut is connected to the first nut seat. The first lead screw is vertically arranged. The output end of the first linear motor is connected to the upper end of the first lead screw. The first lead screw nut is sleeved on the first lead screw.

[0016] Further, the second driving mechanism further includes a second linear motor, a second motor base, a second lead screw, a second lead screw nut, a second nut base, a push plate, and a stirring rod sleeve. The second linear motor is connected to the second bottom plate through the second motor base. The output end of the second linear motor is connected to the second lead screw. The second lead screw nut is sleeved on the second lead screw. The push plate is connected to the second lead screw nut through the second nut base. The push plate is connected through the stirring rod sleeve.

[0017] Further, the second driving mechanism further includes a third lead screw, a third lead screw nut, a third nut base, a third motor base, a third linear motor, and a magnetic rod base. The third linear motor is connected to the second bottom plate. The output shaft of the third linear motor is connected to the third lead screw. The third lead screw nut is connected to the magnetic rod base through the third nut base. The magnetic rod base is connected to a magnetic rod rod. A magnetic rod is connected to the lower end of the magnetic rod rod.

[0018] Further, the second driving mechanism further includes a Z-axis slide rail base, a second linear guide rail, a connecting plate, and a third linear guide rail. The second linear guide rail is vertically arranged on the left side or the right side of the Z-axis slide rail base. One end of the connecting plate is connected to the push plate. The other end of the connecting plate is slidably connected to the second linear guide rail through a slider. The third linear guide rail is vertically arranged on the front side of the Z-axis slide rail base. The magnetic rod base is slidably connected to the third linear guide rail through a slider.

[0019] Further, the third driving mechanism includes a gear of the stirring rod, an idler gear, a driving wheel, and a stirring motor. The gear of the stirring rod is connected to the stirring rod. The idler gear meshes with the gear of the stirring rod and the driving wheel respectively. The output shaft of the stirring motor is connected to the driving wheel.

[0020] The technical effects and advantages of the present utility model:

[0021] 1. The extraction carrier is driven to correspond to the stirring rod by the first driving mechanism. The second driving mechanism is used to drive the stirring rod to extend into the extraction tube with a magnetic rod sleeve, the extraction tube with washing liquid, and the extraction tube with eluent respectively. The third driving mechanism is used to drive the stirring rod to rotate, so as to obtain a pollution-free nucleic acid extract, thereby ensuring the accuracy of nucleic acid detection results.

[0022] Other features and advantages of the present utility model will be described in the subsequent description. And, some of them will become obvious from the description, or can be understood by implementing the present utility model. The purpose and other advantages of the present utility model can be realized and obtained through the structure pointed out in the description and the drawings. Description of the Drawings

[0023] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the accompanying drawings required in the description of the embodiments or the prior art. Obviously, the accompanying drawings in the following description are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other accompanying drawings can also be obtained based on these drawings.

[0024] Figure 1 The structural schematic diagram of the nucleic acid extraction device for preventing cross - contamination according to the embodiment of the present invention is shown;

[0025] Figure 2 shown Figure 1 the structural schematic diagram of another perspective of the nucleic acid extraction device for preventing cross - contamination in

[0026] Figure 3 The structural schematic diagram of the extraction carrier according to the embodiment of the present invention is shown;

[0027] Figure 4 The structural schematic diagram of the extraction cavity door according to the embodiment of the present invention is shown

[0028] Figure 5 The partial structural schematic diagram of the second driving mechanism according to the embodiment of the present invention is shown;

[0029] Figure 6 The structural schematic diagram of another part of the second driving mechanism according to the embodiment of the present invention is shown;

[0030] Figure 7 shown Figure 6 the structural schematic diagram of another perspective of another part of the second driving mechanism in

[0031] Figure 8 The structural schematic diagram of the third driving mechanism according to the embodiment of the present invention is shown.

[0032] Reference numerals: 11, base; 12, extraction chamber; 13, rear side plate; 2, extraction carrier; 21, base; 22, heat insulation plate; 23, pipe seat; 24, heating seat; 25, pressing plate; 3, first driving mechanism; 31, motor; 32, lead screw nut; 33, sliding seat; 34, conduit; 35, bearing seat; 36, linear bearing; 4, second driving mechanism; 411, column; 412, first bottom plate; 413, reinforcing plate; 414, first cross beam; 421, first linear motor; 422, motor bracket; 423, first linear guide rail; 424, limit block; 425, first lead screw nut; 426, first nut seat; 43, second bottom plate; 431, second linear motor; 432, second motor seat; 433, second lead screw; 434, second lead screw nut; 435, second nut seat; 436, push plate; 437, stirring rod sleeve; 441, third linear motor; 442, third motor seat; 443, third lead screw; 444, third lead screw nut; 445, third nut seat; 446, magnetic rod seat; 447, cross plate; 451, Z-axis slide rail seat; 452, second linear guide rail; 453, connecting plate; 454, third linear guide rail; 5, third driving mechanism; 51, gear of stirring rod; 511, stirring rod; 52, idler gear; 53, driving wheel; 54, stirring motor; 55, gear upper cover; 56, upper bearing; 61, extraction pipe with magnetic rod sleeve; 62, extraction pipe with washing solution; 63, extraction pipe with eluent; 71, extraction chamber door; 72, door slide rod; 73, spring; 74, pin; 75, door seat; 81, magnetic rod rod; 82, magnetic rod. Detailed implementation manners

[0033] To make the objectives, technical solutions and advantages of the embodiments of the present utility model clearer, the technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are some but not all of the embodiments of the present utility model. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts shall fall within the protection scope of the present utility model.

[0034] As Figures 1 to 3 shown, a nucleic acid extraction device for preventing cross-contamination according to an embodiment of the present utility model includes a base 11, an extraction chamber 12, an extraction carrier 2, a first driving mechanism 3, a second driving mechanism 4, and a third driving mechanism 5. The extraction chamber 12 is disposed on the base 11. The extraction carrier 2 is configured to be disposed in the extraction chamber 12. The first driving mechanism 3 is disposed on the base 11. The output end of the first driving mechanism 3 passes through the extraction chamber 12 and is connected to the extraction carrier 2. The first driving mechanism 3 is configured to drive the extraction carrier 2 to move in the front-rear direction.

[0035] On the extraction carrier 2, extraction tubes 61 with magnetic rod sleeves, extraction tubes 62 with washing solution, and extraction tubes 63 with elution solution are sequentially distributed in the front-back direction.

[0036] The second driving mechanism 4 is arranged at the upper end of the extraction chamber 12. The output end of the second driving mechanism 4 is used to connect to the stirring rod 511, and the stirring rod 511 is used to connect to the magnetic rod sleeve.

[0037] The third driving mechanism 5 is arranged at the upper end of the extraction chamber 12. The third driving mechanism 5 is used to drive the stirring rod 511 to perform stirring.

[0038] Specifically, the magnetic rod sleeve is clamped into the extraction tube pre-loaded with lysis / binding solution, a certain amount of sample, proteinase K, and magnetic beads.

[0039] Secondly, on the extraction carrier 2, a lysis / binding position, a washing position, and an elution position are sequentially distributed in the front-back direction. The lysis / binding position is provided with an extraction tube 61 with a magnetic rod sleeve, the washing position is provided with an extraction tube 62 with washing solution, and the elution position is provided with an extraction tube 63 with elution solution.

[0040] In this embodiment, the first driving mechanism 3 is used to drive the extraction carrier 2 to move in the front-back direction, so as to push the extraction tube 61 with a magnetic rod sleeve on the extraction carrier 2 to correspond to the stirring rod 511. The second driving mechanism 4 is used to drive the stirring rod 511 to extend into the extraction tube 61 with a magnetic rod sleeve. The third driving mechanism 5 is used to drive the stirring rod 511 with the magnetic rod sleeve to stir the mixed solution in the extraction tube, and complete the work of nucleic acid lysis and nucleic acid binding to magnetic beads of the sample.

[0041] The first driving mechanism 3 is used to drive the extraction carrier 2 to move in the front-back direction, so as to push the extraction tube with washing solution on the extraction carrier 2 to correspond to the stirring rod 511. The second driving mechanism 4 is used to drive the stirring rod 511 to extend into the extraction tube with washing solution. The third driving mechanism 5 is used to drive the stirring rod 511 with the magnetic rod sleeve to stir and mix the mixed solution, and wash the magnetic beads adsorbed with nucleic acid.

[0042] The first driving mechanism 3 is used to drive the extraction carrier 2 to move in the front-back direction, so as to push the extraction tube 63 with elution solution on the extraction carrier 2 to correspond to the stirring rod 511. The second driving mechanism 4 is used to drive the stirring rod 511 to extend into the extraction tube 63 with elution solution. The third driving mechanism 5 is used to drive the stirring rod 511 with the magnetic rod sleeve to stir and mix the mixed solution, and wash the magnetic beads adsorbed with nucleic acid.

[0043] Thus, the first driving mechanism 3 is used to drive the extraction carrier 2 to move in the front-rear direction within the extraction chamber 12, so that the extraction tube 61 with a magnetic rod sleeve, the extraction tube 62 with washing liquid, and the extraction tube 63 with eluent on the extraction carrier 2 sequentially correspond to the stirring rod 511. The second driving mechanism 4 is used to drive the stirring rod 511 to extend into the extraction tube 61 with a magnetic rod sleeve, the extraction tube 62 with washing liquid, and the extraction tube 63 with eluent respectively, and the third driving mechanism 5 is used to drive the stirring rod 511 to rotate, so as to obtain a pollution-free nucleic acid extract, thereby ensuring the accuracy of the nucleic acid detection result.

[0044] Optionally, as Figure 2 and Figure 3 shown, the nucleic acid extraction device for preventing cross-contamination further includes a rear side plate 13, the rear side plate 13 is connected to the rear side surface of the extraction chamber 12. The first driving mechanism 3 includes a motor 31, a lead screw, a lead screw nut 32, a sliding seat 33, and a conduit 34. The motor 31 is arranged on the base 11. The axial direction of the lead screw faces the front-rear direction. One end of the lead screw is connected to the output shaft of the motor 31, and the other end of the lead screw is used to be connected to the rear side plate 13. The lead screw nut 32 is sleeved on the lead screw and connected to the sliding seat 33. One end of the conduit 34 is connected to the sliding seat 33, and the other end of the conduit 34 passes through the extraction chamber 12 and is connected to the extraction carrier 2.

[0045] Specifically, conduits 34 are arranged on both the left and right sides of the sliding seat 33.

[0046] In this embodiment, the motor 31 drives the lead screw to rotate, so that the lead screw nut 32 drives the sliding seat 33 to slide on the lead screw in the front-rear direction. The sliding seat 33 drives the extraction carrier 2 to slide in the front-rear direction through the conduit 34. Thus, by setting the first driving mechanism 3 to include the motor 31, the lead screw, the lead screw nut 32, the sliding seat 33, and the conduit 34, the motor 31 can be used to drive the sliding seat 33 and the conduit 34, thereby driving the extraction carrier 2 to move in the front-rear direction, ensuring the effect of the first driving mechanism 3.

[0047] Optionally, as Figure 3 shown, the first driving mechanism 3 further includes a bearing seat 35 and a linear bearing 36. The bearing seat 35 is connected to the rear side plate 13. The linear bearing 36 is arranged inside the bearing seat 35. The conduit 34 sequentially passes through the linear bearing 36 and the bearing seat 35.

[0048] Specifically, the conduit 34 and the linear bearing 36 are coaxially distributed.

[0049] In this embodiment, by providing a bearing block 35 and a linear bearing 36, the conduit 34 passes through the linear bearing 36 and the bearing block 35 in sequence, so that the conduit 34 can be limited in position to ensure that the conduit 34 moves in the front-rear direction.

[0050] Optionally, as Figure 4 shown, the nucleic acid extraction device for preventing cross-contamination further includes an extraction chamber door 71 and a door slide bar 72. The extraction chamber door 71 is located on the front side of the extraction chamber 12. The rear side of the door slide bar 72 is slidably connected to the conduit 34, and the front side of the door slide bar 72 is used to connect to the extraction chamber door 71;

[0051] The first driving mechanism 3 further includes a spring 73. The spring 73 is disposed inside the conduit 34 and is coaxially distributed with the conduit 34. One end of the spring 73 is connected to the conduit 34, and the other end of the spring 73 is connected to the door slide bar 72.

[0052] Specifically, as Figure 4 shown, the front side of the extraction chamber 12 is open, and the extraction chamber door 71 is used to close the front opening of the extraction chamber 12. Secondly, the nucleic acid extraction device for preventing cross-contamination further includes a pin 74. The pin 74 is connected to the door slide bar 72 and is used to connect the spring 73. In addition, the nucleic acid extraction device for preventing cross-contamination further includes a door seat 75. The door seat 75 is connected to the extraction chamber door 71, and the front side of the door slide bar 72 is connected to the door seat 75.

[0053] In this embodiment, when the extraction chamber door 71 is closed, the extraction carrier 2 moves backward to the inside of the extraction chamber 12 and drives the extraction chamber door 71 to move backward synchronously through the door slide bar 72. When the extraction chamber door 71 docks with the front side of the extraction chamber 12, the extraction chamber door 71 no longer moves backward, while the extraction carrier 2 continues to move backward against the elastic force of the spring 73, so that the extraction carrier 2 is separated from the extraction chamber door 71.

[0054] When the extraction chamber door 71 is opened, the extraction carrier 2 moves forward to the outside of the extraction chamber 12 and drives the extraction chamber door 71 to move forward synchronously through the door slide bar 72. At the same time, since the conduit 34 and the door slide bar 72 are connected by the spring 73, the extraction carrier 2 can be fitted to the extraction chamber door 71.

[0055] Optionally, as Figure 3As shown, the extraction carrier 2 includes a base 21, a heat insulation plate 22, a heating film, thermal conductive silicone grease, a tube base 23, and a heating base 24. The base 21 is connected to the conduit 34. The heat insulation plate 22 is disposed at the upper end of the base 21. The heating bases 24 are disposed on both the front and rear sides of the upper end of the heat insulation plate 22. The tube base 23 is disposed in the middle of the upper end of the heat insulation plate 22. The heating film and the thermal conductive silicone grease are sequentially disposed between the heat insulation plate 22 and the heating base 24 from bottom to top. The front heating base 24 is used to dispose the extraction tube 61 with a magnetic rod sleeve. The tube base 23 is used to dispose the extraction tube 62 with washing liquid. The rear heating base 24 is used to dispose the extraction tube 63 with eluent.

[0056] Specifically, as Figure 3 shown, the extraction carrier 2 further includes a pressing plate 25. The pressing plate 25 is disposed at the upper ends of the tube base 23 and the heating base 24 to limit the tube base 23 and the heating base 24 and prevent the tube base 23 and the heating base 24 from detaching from the heat insulation plate 22.

[0057] In this embodiment, the front and rear heating films heat the two heating bases 24 through the thermal conductive silicone grease to heat the extraction tube 61 with a magnetic rod sleeve and the extraction tube 63 with eluent. The two heating films and the heating bases 24 work independently. The middle tube base 23 is at room temperature and is used to place the extraction tubes for washing. One or more room temperature tube bases 23 can be provided according to the needs of the extraction reagent.

[0058] Secondly, the heat insulation plate 22 is provided to prevent the temperature of the heating base 24 from being transferred to the tube base 23. And the base 21 is provided to facilitate the connection with the conduit 34 through the base 21.

[0059] Optionally, as Figure 5 and Figure 6 shown, the second driving mechanism 4 includes a column 411, a first base plate 412, a first lead screw, a first lead screw nut 425, a first nut seat 426, a second base plate 43, and a first linear motor 421;

[0060] The first base plate 412 is connected to the base 11 through the column 411. The first linear motor 421 is disposed on the first base plate 412. The first nut seat 426 is connected to the second base plate 43. The first lead screw nut 425 is connected to the first nut seat 426. The first lead screw is vertically disposed. The output end of the first linear motor 421 is connected to the upper end of the first lead screw. The first lead screw nut 425 is sleeved on the first lead screw.

[0061] Specifically, as Figure 5As shown in the figure, two columns 411 are arranged on the left and right sides of the base 11, and the first bottom plate 412 is located at the upper ends of the columns 411. The second driving mechanism 4 further includes a reinforcing plate 413 and a first cross beam 414. Reinforcing plates 413 are arranged on both the left and right sides of the first bottom, and the left and right ends of the first cross beam 414 are respectively connected to a column 411. The first bottom plate 412 is used to be arranged at the upper end of the first cross beam 414. A motor bracket 422 is arranged on the rear side of the first bottom plate 412, and a first linear motor 421 is arranged at the upper end of the motor bracket 422.

[0062] Secondly, as Figure 5 shown, the second driving mechanism 4 further includes a first linear guide rail 423 and a limit block 424. The first linear guide rail 423 is vertically arranged on the front side of the first bottom plate 412. A slider is arranged on the rear side of the second bottom plate 43, and the slider is slidably connected to the first linear guide rail 423. A limit block 424 is arranged at the upper end of the first linear guide rail 423, and the limit block 424 is used to abut against the slider.

[0063] In this embodiment, the first linear motor 421 is used to drive the first lead screw to rotate, so that the first lead screw nut 425, the first nut seat 426 and the second bottom plate 43 move in the vertical direction, thereby driving all the structures connected to the second bottom plate 43 to move vertically.

[0064] Secondly, when the second bottom plate 43 moves in the vertical direction, the second bottom plate 43 moves relative to the first linear guide rail 423 on the first bottom plate 412 through the slider, which can ensure the stability of the movement of the second bottom plate 43.

[0065] Optionally, as Figure 6 shown, the second driving mechanism 4 further includes a second linear motor 431, a second motor seat 432, a second lead screw 433, a second lead screw nut 434, a second nut seat 435, a push plate 436 and a stirring rod sleeve 437. The second linear motor 431 is connected to the second bottom plate 43 through the second motor seat 432. The output end of the second linear motor 431 is connected to the second lead screw 433. The second lead screw nut 434 is sleeved on the second lead screw 433. The push plate 436 is connected to the second lead screw nut 434 through the second nut seat 435, and the push plate 436 is connected through the stirring rod sleeve 437.

[0066] In this embodiment, the second linear motor 431 is used to drive the second lead screw 433 to rotate, so that the second lead screw nut 434, the second nut seat 435 and the push plate 436 move in the vertical direction, thereby driving the stirring rod sleeve 437 on the push plate 436 to move in the vertical direction. When the magnetic rod sleeve and the extraction tube are clamped, the extraction tube is pressed by the stirring rod sleeve 437 to separate the magnetic rod sleeve from the extraction tube.

[0067] Optionally, as shown in Figures 6 to 8 shown, the second driving mechanism 4 further includes a third lead screw 443, a third lead screw nut 444, a third nut seat 445, a third motor seat 442, a third linear motor 441, and a magnetic rod seat 446. The third linear motor 441 is connected to the second base plate 43. The output shaft of the third linear motor 441 is connected to the third lead screw 443. The third lead screw nut 444 is connected to the magnetic rod seat 446 through the third nut seat 445. The magnetic rod seat 446 is connected to a magnetic rod 81. A magnetic rod 82 is connected to the lower end of the magnetic rod 81.

[0068] Specifically, the second driving mechanism 4 further includes a third motor seat 442. The third linear motor 441 is connected to the second base plate 43 through the third motor seat 442.

[0069] In this embodiment, the third linear motor 441 is used to drive the third lead screw 443 to rotate, so that the third lead screw nut 444, the third nut seat 445, and the magnetic rod seat 446 move in the vertical direction, thereby driving the magnetic rod 81 on the magnetic rod seat 446 and the magnetic rod 82 on the magnetic rod 81 to move in the vertical direction. This is used to insert the magnetic rod 82 through the stirring rod sleeve 437 into the extraction tube 61 with a magnetic rod sleeve and make the magnetic rod 82 fit with the magnetic rod sleeve. By moving the magnetic rod 82 downward, magnetic beads adsorbed with nucleic acids and impurities are attracted from the liquid surface of the extraction tube to the bottom of the extraction tube. The magnetic beads adsorbed with nucleic acids and impurities will be concentrated at the bottom of the magnetic rod sleeve. When the magnetic rod 82 moves upward, the magnetic rod sleeve is lifted away from the liquid surface of the extraction tube, and the magnetic beads adsorbed with nucleic acids and impurities at the bottom of the magnetic rod sleeve are simultaneously lifted and separated from the extraction tube and the mixed liquid at the lysis / binding position.

[0070] Optionally, as shown in Figures 6 to 8 shown, the second driving mechanism 4 further includes a Z-axis slide rail seat 451, a second linear guide rail 452, a connecting plate 453, and a third linear guide rail 454. The second linear guide rail 452 is vertically arranged on the left or right side surface of the Z-axis slide rail seat 451. One end of the connecting plate 453 is connected to the push plate 436, and the other end of the connecting plate 453 is slidably connected to the second linear guide rail 452 through a slider. The third linear guide rail 454 is vertically arranged on the front side surface of the Z-axis slide rail seat 451. The magnetic rod seat 446 is slidably connected to the third linear guide rail 454 through a slider.

[0071] Specifically, as shown in Figures 6 to 8 shown, the Z-axis slide rail seats 451 are arranged at both the left and right ends of the front side surface of the second bottom. The second driving mechanism 4 further includes a cross plate 447. The cross plate 447 is arranged at the upper ends of the two Z-axis slide rail seats 451. The left and right ends of the cross plate 447 are respectively connected to one of the Z-axis slide rail seats 451, and the third linear guide rails 454 are arranged on both of the Z-axis slide rail seats 451.

[0072] In this embodiment, by providing a Z-axis slide rail base 451 and a third linear guide rail 454 on the Z-axis slide rail base 451, and by providing a slider on the magnetic rod base 446, the magnetic rod base 446 is slidably connected to the third linear guide rail 454 through the slider, so that the movement of the magnetic rod base 446 can be limited.

[0073] By providing a second linear guide rail 452 on the Z-axis slide rail base 451 and a slider on the connecting plate 453, the connecting plate 453 is slidably connected to the second linear guide rail 452 through the slider, so that the movement of the push plate 436 can be limited.

[0074] Optionally, as Figure 8 shown, the third driving mechanism 5 includes a gear 51 of the stirring rod, an idler gear 52, a driving wheel 53 and a stirring motor 54. The gear 51 of the stirring rod is connected to the stirring rod 511. The idler gear 52 meshes with the gear 51 of the stirring rod and the driving wheel 53 respectively. The output shaft of the stirring motor 54 is connected to the driving wheel 53.

[0075] Specifically, a plurality of gears are provided, and the plurality of gears are distributed in sequence in the left-right direction, and two adjacent gears mesh with each other. The third driving mechanism 5 further includes a gear upper cover 55 and an upper bearing 56. An upper bearing 56 is provided on each gear 51 of the stirring rod. Each gear 51 of the stirring rod is connected to a stirring rod 511. The stirring rod 511 is located inside the stirring rod sleeve 437. The gear upper cover 55 covers the plurality of gears 51 of the stirring rod, the idler gear 52 and the driving wheel 53, and is connected to the second bottom plate 43. The stirring motor 54 is connected to the gear upper cover 55.

[0076] In this embodiment, the driving wheel 53 is driven to rotate by the stirring motor 54. By using the idler gear 52 to mesh with the driving wheel 53 and the gear 51 of the stirring rod, the gear 51 of the stirring rod is driven to rotate, so as to drive the stirring rod 511 connected to the gear 51 of the stirring rod to rotate, so that the stirring rod 511 stirs the liquid in the extraction tube.

[0077] Although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A nucleic acid extraction device for preventing cross-contamination, characterized in that, It includes a base (11), an extraction chamber (12), an extraction carrier (2), a first driving mechanism (3), a second driving mechanism (4), and a third driving mechanism (5). The extraction chamber (12) is disposed on the base (11). The extraction carrier (2) is used to be disposed within the extraction chamber (12). The first driving mechanism (3) is disposed on the base (11). The output end of the first driving mechanism (3) passes through the extraction chamber (12) and is connected to the extraction carrier (2). The first driving mechanism (3) is used to drive the extraction carrier (2) to move in the front-rear direction. On the extraction carrier (2), extraction tubes (61) with magnetic rod sleeves, extraction tubes (62) with washing liquid, and extraction tubes (63) with eluent are sequentially distributed in the front-rear direction. The second driving mechanism (4) is disposed at the upper end of the extraction chamber (12). The output end of the second driving mechanism (4) is used to be connected to a stirring rod (511). The stirring rod (511) is used to be sleeved with a magnetic rod (82). The third driving mechanism (5) is disposed at the upper end of the extraction chamber (12). The third driving mechanism (5) is used to drive the stirring rod (511) to stir.

2. The nucleic acid extraction device for preventing cross-contamination according to claim 1, wherein It further includes a rear side plate (13). The rear side plate (13) is connected to the rear side of the extraction chamber (12). The first driving mechanism (3) includes a motor (31), a lead screw, a lead screw nut (32), a sliding seat (33), and a conduit (34). The motor (31) is disposed on the base (11). The axial direction of the lead screw is towards the front-rear direction. One end of the lead screw is connected to the output shaft of the motor (31). The other end of the lead screw is used to be connected to the rear side plate (13). The lead screw nut (32) is sleeved on the lead screw and is connected to the sliding seat (33). One end of the conduit (34) is connected to the sliding seat (33). The other end of the conduit (34) passes through the extraction chamber (12) and is connected to the extraction carrier (2).

3. The nucleic acid extraction device for preventing cross-contamination according to claim 2, wherein, The first driving mechanism (3) further includes a bearing seat (35) and a linear bearing (36). The bearing seat (35) is connected to the rear side plate (13). The linear bearing (36) is disposed within the bearing seat (35). The conduit (34) sequentially passes through the linear bearing (36) and the bearing seat (35).

4. The nucleic acid extraction device for preventing cross-contamination according to claim 3, characterized in that It further includes an extraction chamber door (71) and a door slide rod (72). The extraction chamber door (71) is located at the front side of the extraction chamber (12). The rear side of the door slide rod (72) is slidably connected to the conduit (34). The front side of the door slide rod (72) is used to be connected to the extraction chamber door (71). The first driving mechanism (3) further includes a spring (73). The spring (73) is disposed within the conduit (34) and is coaxially distributed with the conduit (34). One end of the spring (73) is connected to the conduit (34). The other end of the spring (73) is connected to the door slide rod (72).

5. The nucleic acid extraction device for preventing cross-contamination according to claim 4, wherein, The extraction carrier (2) includes a base (21), a heat insulation plate (22), a heating film, thermal conductive silicone grease, a tube base (23), and a heating base (24). The base (21) is connected to the conduit (34). The heat insulation plate (22) is disposed at the upper end of the base (21). Heating bases (24) are disposed on both the front and rear sides of the upper end of the heat insulation plate (22). The tube base (23) is disposed in the middle of the upper end of the heat insulation plate (22). The heating film and the thermal conductive silicone grease are sequentially disposed between the heat insulation plate (22) and the heating base (24) from bottom to top. The front heating base (24) is used for disposing an extraction tube (61) with a magnetic rod sleeve. The tube base (23) is used for disposing an extraction tube (62) with washing liquid. The rear heating base (24) is used for disposing an extraction tube (63) with eluent.

6. The nucleic acid extraction device for preventing cross-contamination according to claim 1, characterized in that, The second driving mechanism (4) includes a column (411), a first base plate (412), a first lead screw, a first lead screw nut (425), a first nut seat (426), a second base plate (43), and a first linear motor (421); The first base plate (412) is connected to the base (11) through the column (411). The first linear motor (421) is disposed on the first base plate (412). The first nut seat (426) is connected to the second base plate (43). The first lead screw nut (425) is connected to the first nut seat (426). The first lead screw is vertically disposed. The output end of the first linear motor (421) is connected to the upper end of the first lead screw. The first lead screw nut (425) is sleeved on the first lead screw.

7. The nucleic acid extraction device for preventing cross-contamination according to claim 6, wherein The second driving mechanism (4) further includes a second linear motor (431), a second motor seat (432), a second lead screw (433), a second lead screw nut (434), a second nut seat (435), a push plate (436), and a stirring rod sleeve (437). The second linear motor (431) is connected to the second base plate (43) through the second motor seat (432). The output end of the second linear motor (431) is connected to the second lead screw (433). The second lead screw nut (434) is sleeved on the second lead screw (433). The push plate (436) is connected to the second lead screw nut (434) through the second nut seat (435). The push plate (436) is connected through the stirring rod sleeve (437).

8. The nucleic acid extraction device for preventing cross-contamination according to claim 7, wherein The second driving mechanism (4) further includes a third lead screw (443), a third lead screw nut (444), a third nut seat (445), a third motor seat (442), a third linear motor (441), and a magnetic rod seat (446). The third linear motor (441) is connected to the second base plate (43), the output shaft of the third linear motor (441) is connected to the third lead screw (443), the third lead screw nut (444) is connected to the magnetic rod seat (446) through the third nut seat (445), the magnetic rod seat (446) is connected to a magnetic rod (81), and a magnetic bar (82) is connected to the lower end of the magnetic rod (81).

9. The nucleic acid extraction device for preventing cross-contamination according to claim 8, characterized in that, The second driving mechanism (4) further includes a Z-axis slide rail seat (451), a second linear guide rail (452), a connecting plate (453), and a third linear guide rail (454). The second linear guide rail (452) is vertically disposed on the left or right side surface of the Z-axis slide rail seat (451). One end of the connecting plate (453) is connected to the push plate (436), and the other end of the connecting plate (453) is slidably connected to the second linear guide rail (452) through a slider. The third linear guide rail (454) is vertically disposed on the front side surface of the Z-axis slide rail seat (451), and the magnetic rod seat (446) is slidably connected to the third linear guide rail (454) through a slider.

10. The nucleic acid extraction device for preventing cross-contamination according to claim 1, wherein The third driving mechanism (5) includes a gear (51) of the stirring rod, an idler gear (52), a driving wheel (53), and a stirring motor (54). The gear (51) of the stirring rod is connected to the stirring rod (511). The idler gear (52) is meshed with the gear (51) of the stirring rod and the driving wheel (53) respectively, and the output shaft of the stirring motor (54) is connected to the driving wheel (53).

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