Electrochemiluminescence analysis device

Abstract

The utility model discloses an electrochemical luminescence analysis device, which relates to the technical field of detection instruments and comprises a shell, a partition plate, a connector, an electrochemical excitation device and a light intensity detection device. The partition plate is arranged in the shell and divides the shell into a first cavity and a second cavity; the connector is arranged on the partition plate, the shell, the partition plate and the connector are integrally formed, and the connector can be connected with an electrode of the reaction tank; the electrochemical excitation device can be connected with the connector; the electrochemical excitation device can apply excitation voltage to the reaction tank to excite a sample to generate an electrochemical luminescence reaction, and the electrochemical excitation device can collect an electric signal generated when the sample is subjected to an electrochemical reaction; the detection end of the light intensity detection device is arranged opposite to the light through hole, and the light intensity detection device can collect light signals generated when the sample is subjected to electrochemical reaction through the light through hole. The electrochemical luminescence analysis device disclosed by the utility model is portable and high in detection accuracy.

Description

Technical Field

[0001] The utility model relates to the technical field of detection instruments, in particular to an electrochemiluminescence analysis device. Background Art

[0002] Electrochemiluminescence technology (ECL) is a luminescence technology based on electrochemical principles. Its working principle is to apply a voltage in an electrolyte solution to cause an oxidation-reduction reaction on the electrode, thereby activating the luminescent substance in the solution to produce a luminescence phenomenon. It has the characteristics of high energy conversion efficiency, strong controllability, fast response speed, good stability, and reusability. Electrochemiluminescence technology is widely used in the fields of biomedicine, environmental monitoring, medical imaging technology, and nitrate compounds.

[0003] An electrochemiluminescence analysis device is a device that uses electrochemiluminescence technology for detection. Most of the existing electrochemiluminescence analysis devices do not consider the portability of the device. For example, the electrochemiluminescence analysis device with the model BG-gdsAUTO710pro has a large volume and is not convenient to use. Summary of the Utility Model

[0004] The purpose of the utility model is to provide an electrochemiluminescence analysis device to solve the problems existing in the prior art, which can improve the portability and mobility of the electrochemiluminescence analysis device and can improve the accuracy of detection.

[0005] To achieve the above purpose, the utility model provides the following scheme:

[0006] The utility model provides an electrochemiluminescence analysis device, including:

[0007] A housing, on one outer wall of the housing, there is an installation groove, and on the inner bottom wall of the installation groove, there is a light passing hole. The installation groove is used for placing a reaction cell;

[0008] A partition, the partition is arranged in the housing and divides the housing into a first cavity and a second cavity;

[0009] A connector, the connector is arranged on the partition, and the housing, the partition and the connector are integrally formed. The connector can be connected to the electrode of the reaction cell;

[0010] An electrochemical excitation device, the electrochemical excitation device is arranged on the partition, the electrochemical excitation device is arranged in the first cavity, and the electrochemical excitation device can be connected to the connector; the electrochemical excitation device can apply an excitation voltage to the reaction cell to stimulate the sample to undergo an electrochemiluminescence reaction, and the electrochemical excitation device can collect the electrical signal generated when the sample undergoes an electrochemical reaction;

[0011] A light intensity detection device is disposed on the partition plate and is located in the second cavity. The detection end of the light intensity detection device is disposed opposite to the light through hole, and the light intensity detection device can collect the light signal generated when the sample undergoes an electrochemical reaction through the light through hole.

[0012] Preferably, it further includes a bracket. The bracket, the housing, the partition plate and the connector are integrally formed. The bracket is disposed in the first cavity, on the partition plate, and the electrochemical excitation device is fixedly connected to the bracket.

[0013] Preferably, it further includes a fixing frame. The fixing frame, the housing, the partition plate and the connector are integrally formed. The fixing frame is disposed in the second cavity, on the partition plate, and the light intensity detection device is fixed to the partition plate through the fixing frame.

[0014] Preferably, it further includes a slot cover disposed at the entrance of the installation slot. When the slot cover is closed, it can completely seal the entrance of the installation slot and form a darkroom when the slot cover is closed.

[0015] Preferably, the bracket, the housing, the partition plate and the connector are integrally formed by 3D printing technology.

[0016] Preferably, the fixing frame, the housing, the partition plate and the connector are integrally formed by 3D printing technology.

[0017] Preferably, it further includes an electromagnet sleeved outside the light intensity detection device, and the electromagnet is fixedly connected to the fixing frame.

[0018] Preferably, it further includes a power supply device disposed in the first cavity. The connector is disposed at one end of the partition plate close to the installation slot, the power supply device is disposed at the other end of the partition plate far from the installation slot, the electrochemical excitation device is disposed between the connector and the power supply device, and the power supply device is connected to the electrochemical excitation device, the light intensity detection device and the electromagnet.

[0019] Preferably, the light intensity detection device includes a photomultiplier tube, a high-voltage module, and a step-down module. The photomultiplier tube, the high-voltage module, and the step-down module are all disposed in the second cavity. The photomultiplier tube is connected to one end of the partition plate close to the installation groove, the step-down module is connected to one end of the partition plate away from the installation groove, the high-voltage module is disposed between the light intensity detection device and the step-down module and is connected to the partition plate. The step-down module is connected to the high-voltage module, the high-voltage module is connected to the photomultiplier tube, and the photomultiplier tube is disposed opposite to the light passing hole.

[0020] Preferably, it further includes a detection device, and both the electrochemical excitation device and the light intensity detection device can be signal-connected to the detection device.

[0021] The present utility model has achieved the following technical effects compared with the prior art:

[0022] The present utility model provides an electrochemiluminescence analysis device, which includes a housing, a partition plate, a connector, an electrochemical excitation device, and a light intensity detection device. An installation groove is provided on an outer wall of the housing; the partition plate is disposed in the housing and divides the housing into a first cavity and a second cavity; the connector is disposed on the partition plate, and the housing, the partition plate, and the connector are integrally formed. The connector can be connected to the electrode of the reaction cell; the electrochemical excitation device can be connected to the connector; the electrochemical excitation device can apply an excitation voltage to the reaction cell to stimulate the sample to undergo an electrochemiluminescence reaction, and the electrochemical excitation device can collect the electrical signals generated when the sample undergoes an electrochemical reaction; the detection end of the light intensity detection device is disposed opposite to the light passing hole, and the light intensity detection device can collect the light signals generated when the sample undergoes an electrochemical reaction through the light passing hole.

[0023] The housing, the partition plate, and the connector of the present utility model are integrally formed, and no additional connectors are required to connect between multiple components, making the product more integrated, so that the volume of the electrochemiluminescence analysis device can be smaller, thereby improving the portability and mobility of the electrochemiluminescence analysis device. At the same time, both the electrochemical excitation device and the light intensity detection device of the present utility model can collect the signals of the electrochemiluminescence reaction and transmit them to the detection device for detection, which is beneficial to improving the accuracy of detection. Description of the Drawings

[0024] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present utility model. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0025] Figure 1Structural schematic of the electrochemiluminescence analysis device provided by the present utility model Figure 1 ;

[0026] Figure 2 Structural schematic of the electrochemiluminescence analysis device provided by the present utility model Figure 2 ;

[0027] Figure 3 Are the electrochemiluminescence intensity values when the TAP concentration is 0 μM, 50 μM, 200 μM, 500 μM, and 1500 μM;

[0028] Figure 4 Is the comparative diagram of the electrochemiluminescence intensity between the electrochemiluminescence analysis device of the present utility model and a traditional electrochemical workstation during the quantitative detection of TPA;

[0029] In the figure: 100, electrochemiluminescence analysis device; 1, housing; 101, installation groove; 102, light passing hole; 103, power cord hole; 104, detector wire hole; 2, partition; 201, first cavity; 202, second cavity; 203, connection wire hole; 3, connector; 4, electrochemistry excitation device; 401, microcontroller; 402, three - electrode signal conditioning circuit; 5, light intensity detection device; 501, photomultiplier tube; 502, high - voltage module; 503, step - down module; 6, bracket; 7, fixing frame; 8, slot cover; 9, electromagnet; 10, power supply device; 11, detection device; 12, magnet control switch; 13, three - electrode contact; 14, support frame. Detailed implementation manners

[0030] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0031] The purpose of the present utility model is to provide an electrochemiluminescence analysis device to solve the problems existing in the prior art, which can improve the portability and mobility of the electrochemiluminescence analysis device and can improve the accuracy of detection.

[0032] To make the above - mentioned objects, features, and advantages of the present utility model more obvious and understandable, the present utility model will be further described in detail below in conjunction with the drawings and specific implementation manners.

[0033] As Figure 1-2As shown in the figure, the present utility model provides an electrochemiluminescence analysis device 100, which includes: a housing 1, a partition 2, a connector 3, an electrochemical excitation device 4, and a light intensity detection device 5. An installation groove 101 is provided on an outer wall of the housing 1, and a light passing hole 102 is provided on the inner bottom wall of the installation groove 101. The installation groove 101 is used for placing a reaction cell; the partition 2 is arranged inside the housing 1 and divides the housing 1 into a first cavity 201 and a second cavity 202; the connector 3 is arranged on the partition 2, and the housing 1, the partition 2, and the connector 3 are integrally formed. It should be noted that the installation groove 101 is formed synchronously during the integral forming process, and the connector 3 can be connected to the electrodes of the reaction cell; the electrochemical excitation device 4 is arranged on the partition 2, the electrochemical excitation device 4 is arranged in the first cavity 201, and the electrochemical excitation device 4 can be connected to the connector 3. The electrochemical excitation device 4 is connected to the reaction cell electrodes through the connector 3; the electrochemical excitation device 4 can apply an excitation voltage to the reaction cell to stimulate the sample to undergo an electrochemiluminescence reaction, and the electrochemical excitation device 4 can collect the electrical signals generated when the sample undergoes an electrochemical reaction; the light intensity detection device 5 is arranged on the partition 2, and the light intensity detection device 5 is arranged in the second cavity 202; the detection end of the light intensity detection device 5 is arranged opposite to the light passing hole 102, and the light intensity detection device 5 can collect the light signals generated when the sample undergoes an electrochemical reaction through the light passing hole 102. It should be noted that the three-electrode conductive area of the reaction cell is arranged opposite to the lens of the light intensity detection device 5.

[0034] The housing 1, the partition 2, and the connector 3 of the present utility model are integrally formed, and no additional connecting parts are required to connect between multiple components, making the product more integrated, so that the volume of the electrochemiluminescence analysis device 100 can be smaller, thereby improving the portability and mobility of the electrochemiluminescence analysis device 100. At the same time, both the electrochemical excitation device 4 and the light intensity detection device 5 of the present utility model can collect the signals of the electrochemiluminescence reaction and transmit them to the detection device 11 for detection, which is beneficial to improving the accuracy of detection.

[0035] In the present utility model, a bracket 6 is further included. The bracket 6, the housing 1, the partition 2, and the connector 3 are integrally formed. The bracket 6 is arranged in the first cavity 201, the bracket 6 is arranged on the partition 2, and the electrochemical excitation device 4 is fixedly connected to the bracket 6.

[0036] In the present utility model, a fixing frame 7 is further included. The fixing frame 7, the housing 1, the partition 2, and the connector 3 are integrally formed. The fixing frame 7 is arranged in the second cavity 202, the fixing frame 7 is arranged on the partition 2, and the light intensity detection device 5 is fixed to the partition 2 through the fixing frame 7.

[0037] In the present utility model, a slot cover 8 is further included. The slot cover 8 is arranged at the entrance of the installation slot 101. When the slot cover 8 is closed, it can completely seal the entrance of the installation slot 101, and when the slot cover 8 is closed, it can form a darkroom in the installation slot 101. The electrochemiluminescence analysis device 100 of the present utility model does not necessarily need to be detected in a specific darkroom, and can meet the detection requirements of various scenarios such as mobile laboratories, community clinics, household use, the field, ambulances, etc., which is convenient for application.

[0038] As a preferred embodiment, the bracket 6, the housing 1, the partition 2 and the connector 3 are integrally formed by 3D printing technology.

[0039] As a preferred embodiment, the fixing frame 7, the housing 1, the partition 2 and the connector 3 are integrally formed by 3D printing technology.

[0040] As a more preferred embodiment, the fixing frame 7, the bracket 6, the housing 1, the partition 2 and the connector 3 are integrally formed by 3D printing technology. The fixing frame 7, the bracket 6, the housing 1, the partition 2 and the connector 3 are all made of polylactic acid (PLA) black material.

[0041] In the present utility model, an electromagnet 9 is further included. The electromagnet 9 is sleeved outside the light intensity detection device 5, and the electromagnet 9 is fixedly connected to the fixing frame 7. The electromagnet 9 can generate an adsorption effect on the magnetic beads in the reaction cell through the magnetic field, and then control the magnetic beads, such as controlling the movement of the magnetic beads, which is convenient for sample detection.

[0042] In the present utility model, a power supply device 10 is further included. The power supply device 10 is arranged in the first cavity 201. The connector 3 is arranged at one end of the partition 2 close to the installation slot 101, the power supply device 10 is arranged at the end of the partition 2 far from the installation slot 101, the electrochemistry excitation device 4 is arranged between the connector 3 and the power supply device 10, the power supply device 10 is connected to the electrochemistry excitation device 4, the light intensity detection device 5 and the electromagnet 9, and the power supply device 10 can supply power to the electrochemistry excitation device 4, the light intensity detection device 5 and the electromagnet 9. Since the reaction cell electrode, the connector 3 and the electrochemistry excitation device 4 are connected in sequence, the connector 3 is arranged close to the reaction cell electrode, and the electrochemistry excitation device 4 is arranged close to the connector 3, making the structure more compact.

[0043] It should be noted that a connection wire hole 203 is arranged on the partition 2. The power supply cable of the power supply device 10 passes through the connection wire hole 203 and is connected to the light intensity detection device 5, the electromagnet 9 and the step-down module 503. The power supply device 10 is connected to the light intensity detection device 5 through the step-down module 503.

[0044] In the present utility model, the light intensity detection device 5 includes a photomultiplier tube 501, a high-voltage module 502, and a step-down module 503. The photomultiplier tube 501, the high-voltage module 502, and the step-down module 503 are all arranged in the second cavity 202. The photomultiplier tube 501 is connected to one end of the partition plate 2 close to the installation groove 101, the step-down module 503 is connected to the end of the partition plate 2 far from the installation groove 101, the high-voltage module 502 is arranged between the light intensity detection device 5 and the step-down module 503 and is connected to the partition plate 2, the step-down module 503 is connected to the high-voltage module 502, the high-voltage module 502 is connected to the photomultiplier tube 501, and the photomultiplier tube 501 is arranged opposite to the light passing hole 102. Since the photomultiplier tube 501 is used to collect the optical signal when the sample in the reaction cell emits light, the photomultiplier tube 501 is arranged close to the reaction cell; since the photomultiplier tube 501, the high-voltage module 502, and the low-voltage module need to be connected in sequence, the high-voltage module 502 is arranged close to the photomultiplier tube 501, further improving the compactness of the structure. The step-down module 503 controls the high-voltage module 502 to drive the photomultiplier tube 501, which is used to convert the optical signal generated by electrochemiluminescence into an electrical signal and can be used to adjust the sensitivity of the photomultiplier tube 501. The present utility model can use the photomultiplier tube 501 to realize automatic and high-throughput quantitative detection of electrochemiluminescence. It should be noted that the specific structure and working principle of the light intensity detection device 5 are prior art and will not be elaborated here.

[0045] In the present utility model, a detection device 11 is further included. Both the electrochemical excitation device 4 and the light intensity detection device 5 can be signal-connected to the detection device 11. The detection device 11 is used to receive the electrical signals of the electrochemical excitation device 4 and the light intensity detection device 5, process and store the data to achieve detection, and can perform human-machine interface interaction and display of detection results.

[0046] As a preferred embodiment, the connector 3 is a three-electrode contact connector 3, the installation groove 101 is a card slot, the detection device 11 is a computer, and the photomultiplier tube 501 is a photon-counting photomultiplier tube 501.

[0047] As a preferred embodiment, the installation groove 101 is arranged at the top of the housing 1 and on the top of the second cavity 202. The light passing hole 102 is a circular hole, and the diameter of the circular light passing hole 102 is larger than the diameter of the photomultiplier tube 501.

[0048] As a preferred embodiment, a magnet control switch 12 is further included. The magnet control switch 12 is arranged on the outer side wall of the housing 1, and the magnet control switch 12 is connected to the electromagnet 9.

[0049] As a preferred embodiment, the detection device 11 is arranged outside the housing 1. A detector wire hole 104 is provided on the side wall of the housing 1. Output cables of the electrochemical excitation device 4 and the light intensity detection device 5 both pass through the detector wire hole 104 and are connected to the detection device 11.

[0050] As a preferred embodiment, a support frame 14 is provided on the partition plate 2. The connector 3 is in contact with or connected to the support frame 14, and the support frame 14 is used to provide a supporting effect on the connector 3.

[0051] As a preferred embodiment, the electrochemically exciting device 4 is a potentiostatic excitation module, including a microcontroller 401 and a three-electrode signal conditioning circuit 402 connected to each other. The microcontroller 401 and the three-electrode signal conditioning circuit 402 are both fixed on the partition 2 through the bracket 6. The microcontroller 401 is an Arduino UNO R3 based on ATmega328P and can be used to control the output of the potentiostatic excitation module with the required voltage waveform. Among them, the working frequency of the Arduino Uno R3 is 20 MHz, it has 14 digital input / output pins, 6 analog input pins, and 1 USB interface, and can provide stable voltage outputs of 5V and 3.3V. In cooperation with the Arduino IDE (Integrated Development Environment), users can write code programs and upload them to the development board to control the combination of the Arduino Uno R3 with various external hardware devices. The three-electrode signal conditioning circuit 402 includes a D / A conversion module, an A / D conversion module, a signal amplification I / V conversion circuit module, and three-electrode signal output pins. The switching power supply is the input voltage of the buck module 503 (preferably using the TPS5430 chip), and the buck module 503 converts it into the ±9V dual power supply required by the potentiostatic conditioning circuit. The digital-to-analog conversion module (DAC) is controlled by the integrated circuit (I2C) bus of the microcontroller 401 to generate the required waveform voltage, and this voltage acts between the working electrode and the reference of the reaction cell. The output current is obtained through the D / A conversion module, the A / D conversion module, and the signal amplification I / V conversion circuit module, and is displayed on the display module of the detection device 11. The three-electrode signal conditioning circuit 402 uses a 12-bit DAC MCP4725 module and a 16-bit ADS1115 module to achieve a 15-bit resolution, which is equivalent to a resolution of 1.04 μA, meeting many experimental scenarios and having characteristics such as high resolution and fast sampling speed, enabling the device to accurately and quickly collect and process data. The potentiostatic excitation module can precisely control the electrode potential through its internal electrochemical measurement system, thereby triggering and detecting the electrochemiluminescence reaction. The firmware program in the microcontroller 401 is written in C++. This firmware contains electrochemistry detection functions such as cyclic voltammetry (CV), chronoamperometry (CA), and calibration. By running a script program on the computer, receiving commands from the computer and sending data, electrochemistry detection and analysis can be realized. The data collected by the microcontroller 401 is transmitted to the computer for analysis wirelessly or wiredly. The host computer realizes the control of the potentiostatic excitation module by installing the open-source software A on the computer. The host computer software B of the photomultiplier tube 501 can realize the control of the photomultiplier tube 501. The host computer software of the photomultiplier tube 501 uses open-source software to control the potentiostatic module, including the selection of three-electrode electrochemiluminescence and the setting of their respective parameters.It should be noted that the specific structure, working principle of the electrochemical excitation device 4 and the above-mentioned open-source software are all prior arts. For example, an electrochemical excitation device 4 of model PolArStat can be adopted, which will not be elaborated here.

[0052] It should be noted that the potentiostatic excitation module can independently detect the three-electrode electrochemical reaction, that is, the detection device 11 can obtain the detection result only through the electrical signal sent by the potentiostatic excitation module.

[0053] As a preferred embodiment, the power supply device 10 is a switching power supply. A power cord hole 103 is provided on the side wall of the housing 1. The power cord hole 103 is used to connect the switching power supply to an external power supply to achieve power supply. The switching power supply outputs three 12V voltages in parallel and is provided with three voltage output interfaces, which are respectively connected to the potentiostatic excitation module, the light intensity detection unit and the electromagnet 9; among them, one output interface converts the voltage into a ±9V dual-rail power supply through a TPS5430 buck device to supply power to the potentiostatic excitation module, one output interface supplies power to the photomultiplier tube 501 through a 5V buck module 503, and one output interface directly supplies power to the electromagnet 9. The switching power supply 5 has a capacity of 2A, and its appearance is a cuboid with a length of 99mm, a width of 95mm, and a height of 36mm.

[0054] As a preferred embodiment, the reaction cell uses a three-electrode electrochemiluminescence chip as the substrate, and the electrode contacts at its end are pluggable to the connector 3. As a more preferred embodiment, three-electrode contacts 13 are provided on the top of the housing 1. One end of the three-electrode contacts 13 is connected to the electrode of the reaction cell, and the other end is connected to the electrochemical excitation device 4 through the connector 3.

[0055] The electrochemiluminescence analysis device 100 provided by the present utility model can directly detect samples such as blood analytes without centrifugation and filtration treatment, and has important significance in the fields of disease biomarker detection, such as cancer biomarkers, cardiac biomarkers, etc. For the electrochemiluminescence analysis device 100 of the present utility model, it only takes about 30 seconds from light signal collection to sample analysis completion, and the operation is simple and fast and quantitative detection can be achieved without the operation of professional personnel.

[0056] An application embodiment of the electrochemiluminescence analysis device 100 provided by the present utility model is as follows:

[0057] The present utility model adopts the electrochemiluminescence analysis device 100 to quantitatively detect tripropylamine (TPA) using the tris(bipyridine)ruthenium (Ru(bpy)32+) system. The chip of the reaction cell uses a three-electrode electrochemiluminescence chip. The working electrode of the three-electrode electrochemiluminescence chip is ITO, the counter electrode is carbon, and the reference electrode is Ag / AgCl, which is simply called the three-electrode electrochemiluminescence chip. The detection steps are as follows:

[0058] (1) Ru(bpy)32+ is used to label tripropylamine (or its antibody / antigen). A 5 mM Ru(bpy)32+ solution and TPA solutions with different concentrations are mixed in equal volumes so that they can be detected in the electrochemiluminescence reaction.

[0059] (2) The processed sample is injected into the electrochemical cell (or onto the surface of the working electrode). The electrochemiluminescence reaction cell with the three-electrode electrochemiluminescence chip is fixed onto the mounting groove 101. The three electrodes of the three-electrode electrochemiluminescence chip are connected to the three-electrode contact 13, and the slot cover 8 is closed. According to the experimental requirements, the parameters of the potentiostatic excitation module, such as the potential range, scan rate, etc., are set. The potentiostatic excitation module is started for electrochemical scanning to trigger the electrochemiluminescence reaction of Ru(bpy)32+-labeled tripropylamine on the electrode surface.

[0060] (3) Check whether the working electrode of the reaction cell is aligned with the circular light-transmitting hole 102, then start the potentiostatic excitation module to trigger the electrochemiluminescence reaction. The photomultiplier tube 501 detects and records the electrochemiluminescence intensity, which is proportional to the concentration of tripropylamine in the sample.

[0061] (4) After the electrochemiluminescence reaction ends, the analysis results are displayed and stored on the detection device 11. The electrochemiluminescence intensity data recorded by the photomultiplier tube 501 is imported into the data processing software.

[0062] First, using the electrochemiluminescence analysis device 100 of the present utility model, TPA is quantitatively detected through the Ru(bpy)32+ system. The TPA concentrations are set to 0 μM, 100 μM, 500 μM, and 2500 μM. The chronoamperometry program is started in the upper computer software of the potentiostatic excitation module, and the potential is set to a constant voltage of 1.5 V. Figure 3 The bar graphs in... are the measured luminescence intensity values when the TAP concentration is 0 μM, 50 μM, 200 μM, 500 μM, and 1500 μM (the experiment is repeated 5 times). From Figure 3 it can be seen that as the TPA concentration increases from 0 μM to 2500 μM, its ECL intensity value significantly increases from 2.56×10 5 to 19.68×10 5 , which indicates that the electrochemiluminescence analysis device 100 of the present utility model can quantitatively detect TPA under the Ru(bpy)32+ system.

[0063] Secondly, to verify the correlation between the electrochemiluminescence analysis device 100 of the present utility model and the results of the traditional electrochemistry workstation CHI660E, under the same experimental conditions, the electrochemiluminescence analysis device 100 of the present utility model and the traditional electrochemistry workstation are used to detect the same samples (the experiment is repeated 5 times), and the detection results are compared: Figure 4The bar chart in [Figure 0] shows the detection data at TPA concentrations of 0 μM, 50 μM, 200 μM, 500 μM, and 1500 μM. It can be seen from the bar chart that the two have the same trend. At TPA concentrations of 0 μM, 50 μM, 200 μM, 500 μM, and 1500 μM, the ECL intensity values measured by the electrochemiluminescence analysis device 100 of the present utility model are 2.56×10 5 、4.68×10 5 、9.85×10 5 、15.78×10 5 、19.68×10 5 respectively; the ECL intensity values of the traditional electrochemistry workstation CHI660E are 5.74×10 5 、13.69×10 5 、45.69×10 5 、89.67×10 5 、135.89×10 5 respectively. Through fitting with the data processing software origin, the data correlation at the five concentrations is calculated to be 0.9965, and the two have a good correlation, indicating that the detection results obtained by this analyzer are consistent with the detection results of the combination of PMT-CHI660E.

[0064] In the present utility model, specific examples are used to illustrate the principle and implementation manner of the present utility model. The description of the above embodiments is only used to help understand the method and its core idea of the present utility model; at the same time, for those of ordinary skill in the art, according to the idea of the present utility model, there will be changes in the specific implementation manner and application scope. In summary, the content of this specification should not be construed as a limitation to the present utility model.

Claims

1. An electrochemiluminescence analysis device, characterized in that: Comprising: A housing, on one outer wall of which there is provided an installation groove, on the inner bottom wall of which there is provided a light passing hole, and the installation groove is used for placing a reaction cell; A partition board, which is arranged inside the housing and divides the housing into a first cavity and a second cavity; A connector, which is arranged on the partition board, and the housing, the partition board and the connector are integrally formed, and the connector can be connected to the electrode of the reaction cell; An electrochemical excitation device, which is arranged on the partition board and is arranged inside the first cavity, and the electrochemical excitation device can be connected to the connector; the electrochemical excitation device can apply an excitation voltage to the reaction cell to excite the sample to undergo an electrochemiluminescence reaction, and the electrochemical excitation device can collect the electrical signals generated when the sample undergoes an electrochemical reaction; A light intensity detection device, which is arranged on the partition board and is arranged inside the second cavity; the detection end of the light intensity detection device is arranged opposite to the light passing hole, and the light intensity detection device can collect the light signals generated when the sample undergoes an electrochemical reaction through the light passing hole.

2. The electrochemiluminescence analysis device according to claim 1, wherein: It further includes a bracket, the bracket, the housing, the partition board and the connector are integrally formed, the bracket is arranged inside the first cavity, the bracket is arranged on the partition board, and the electrochemical excitation device is fixedly connected to the bracket.

3. The electrochemiluminescence analysis device according to claim 1, wherein: It further includes a fixing frame, the fixing frame, the housing, the partition board and the connector are integrally formed, the fixing frame is arranged inside the second cavity, the fixing frame is arranged on the partition board, and the light intensity detection device is fixed to the partition board through the fixing frame.

4. The electrochemiluminescence analysis device according to claim 1, wherein: It further includes a groove cover, which is arranged at the entrance of the installation groove, and when the groove cover is closed, it can completely seal the entrance of the installation groove, and when the groove cover is closed, it can make the installation groove form a darkroom.

5. The electrochemiluminescence analysis device according to claim 2, characterized in that: The bracket, the housing, the partition board and the connector are integrally formed by 3D printing technology.

6. The electrochemiluminescence analysis device according to claim 3, characterized in that: The fixing frame, the housing, the partition board and the connector are integrally formed by 3D printing technology.

7. The electrochemiluminescence analysis device according to claim 3, characterized in that: It further includes an electromagnet, which is sleeved outside the light intensity detection device, and the electromagnet is fixedly connected to the fixing frame.

8. The electrochemiluminescence analysis device according to claim 7, wherein: It further includes a power supply device, which is arranged inside the first cavity, the connector is arranged at one end of the partition board close to the installation groove, the power supply device is arranged at one end of the partition board far from the installation groove, the electrochemical excitation device is arranged between the connector and the power supply device, and the power supply device is connected to the electrochemical excitation device, the light intensity detection device and the electromagnet.

9. The electrochemiluminescence analysis device according to any one of claims 1-8, characterized in that: The light intensity detection device includes a photomultiplier tube, a high-voltage module, and a step-down module. The photomultiplier tube, the high-voltage module, and the step-down module are all arranged in the second cavity. The photomultiplier tube is connected to one end of the partition plate close to the installation groove, the step-down module is connected to one end of the partition plate far from the installation groove, the high-voltage module is arranged between the light intensity detection device and the step-down module and is connected to the partition plate. The step-down module is connected to the high-voltage module, the high-voltage module is connected to the photomultiplier tube, and the photomultiplier tube is arranged opposite to the light passing hole.

10. The electrochemiluminescence analysis device according to any one of claims 1-8, characterized in that: It further includes a detection device, and both the electrochemical excitation device and the light intensity detection device can be signal-connected to the detection device.

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