Test strip for rapidly detecting various artemisia pollen allergens
By designing test strips that quickly detect multiple Artemisia pollen allergens, and using nano-gold immunochromatography test methods, the complex and time-consuming problems in the existing technology were solved, and the rapid and accurate Artemisia pollen allergen detection was achieved.
Patent Information
- Application Number
- CN202421837763.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-31
- Publication Date
- 2025-07-11
- Estimated Expiration
- 2034-07-31
AI Technical Summary
The prior art is complicated when detecting Artemisia pollen allergen in serum or plasma, requiring professional operation, taking time, and it is difficult to distinguish the specific Artemisia pollen allergen source, resulting in inaccurate detection.
A test strip was designed to quickly detect multiple Artemisia pollen allergens. The nano-gold immunochromatography test method was used to complete the experiment by adding specimens in one step. The detection lines and nano-gold tracer substances were used at intervals on the NC membrane, combined with the biotin-affinin amplification reaction system, to achieve accurate detection of different Artemisia pollen allergens.
The operation process is simplified, the detection time is shortened to 5-30 minutes, the detection sensitivity and specificity are improved, and the accurate detection of different sources of pollen allergens of Artemisia pollen are achieved.
Smart Images
Figure CN223091967U_ABST
Abstract
Description
Technical field
[0001] The utility model relates to the technical field of medical inspection, in particular to a test strip for rapidly detecting multiple Artemisia pollen allergens. Background technique
[0002] In recent years, the number of patients with seasonal allergic rhinitis (AR) has been increasing year by year, and Artemisia pollen (hereinafter referred to as "Artemisia pollen") has become the main inhalant allergen in northern and western China.
[0003] When detecting specific IgE antibodies of Artemisia pollen allergens in serum or plasma, the prior art usually adopts immunoblotting. In this method, multiple allergens are coated on a solid-phase membrane strip in the form of lines to form a test strip, and serum or plasma separated from venous blood collection is used as a test specimen. The specific steps include: adding the specimen in sequence and reacting for more than half an hour, washing, then adding reagent 1, reacting for more than half an hour, washing, then adding reagent 2, reacting and washing again, and finally observing the color development of each strip of the test strip through an enzyme-catalyzed substrate color reaction to judge the allergen type of the patient. Obviously, this method requires professional inspection technicians to operate, with high difficulty. Especially in the washing link, incomplete washing of the test strip will lead to false positive results. The entire reaction process takes about 2 hours, plus the sample loading and washing links, the total time consumption exceeds 3 hours. In addition, when detecting Artemisia pollen allergens by this method, Artemisia combination and Ambrosia artemisiifolia mixed antigen are often coated on one strip. Even if the experimental result is positive, it is impossible to determine which Artemisia pollen the patient is specifically allergic to.
[0004] The operation steps of the existing method are complex, especially the washing link, which is prone to operation errors; in addition, venous blood collection and serum separation need to be completed by professional medical staff, increasing the operation difficulty and labor cost. The existing method takes a long time, and the whole process from sample processing to result interpretation takes more than 3 hours. The existing method is difficult to distinguish the specific source of Artemisia pollen allergens, resulting in inaccurate detection.
[0005] Therefore, there is an urgent need to design a test strip for rapidly detecting multiple Artemisia pollen allergens. Content of the utility model
[0006] The purpose of the utility model is to provide a test strip for rapidly detecting multiple Artemisia pollen allergens.
[0007] A test strip for rapidly detecting multiple Artemisia pollen allergens, comprising a test strip body and an outer housing disposed outside the test strip body. The test strip body includes a PVC bottom plate and a solid-phase reaction membrane disposed on the PVC bottom plate. The solid-phase reaction membrane includes a secondary antibody pad, a nano-gold pad, and an NC membrane. The NC membrane is fixedly disposed in the middle of the top surface of the PVC bottom plate. The top surface of the NC membrane is sequentially and spacedly provided with a T1 detection line, a T2 detection line, a T3 detection line, and a quality control line from left to right. The left side of the NC membrane is sequentially provided with a nano-gold pad, a secondary antibody pad, and a sample pad that overlap each other. The nano-gold pad is disposed on the left side of the NC membrane and partially overlaps with the NC membrane. The secondary antibody pad is disposed on the left side of the nano-gold pad and partially overlaps with the nano-gold pad. The sample pad is disposed on the left side of the secondary antibody pad and partially overlaps with the secondary antibody pad. The right side of the NC membrane is provided with a water absorption pad that partially overlaps with it.
[0008] Further, the T1 detection line is coated with Artemisia argyi allergen extract, the T2 detection line is coated with Artemisia sieversiana allergen extract, the T3 detection line is coated with Artemisia annua allergen extract, and the quality control line is coated with activated biotin.
[0009] Further, the secondary antibody pad is coated with goat anti-human IgE secondary antibody labeled with activated biotin, the nano-gold pad is coated with nano-gold particles labeled with streptavidin, and the NC membrane is a nitrocellulose membrane.
[0010] Further, the secondary antibody pad is coated with goat anti-human IgE secondary antibody labeled with activated biotin, the nano-gold pad is coated with nano-gold particles labeled with streptavidin, and the NC membrane is a nitrocellulose membrane.
[0011] Further, a sample addition hole matching the sample pad is provided on the left side of the top surface of the outer housing.
[0012] Further, an observation window matching the NC membrane is provided in the middle of the top surface of the outer housing.
[0013] In summary, the present utility model has the following beneficial effects:
[0014] The utility model adopts the nano - gold immunochromatographic test method, simplifies the operation process, and only needs to add the specimen once to complete the experiment, avoiding complex steps such as adding reagents and washing multiple times, greatly reducing the operation difficulty. By using the nano - gold immunochromatographic test method, the detection time is shortened to 5 - 30 minutes to judge the result, and the detection speed is fast. On the NC membrane, the T1 test line coated with Artemisia argyi allergen extract, the T2 test line coated with Artemisia sieversiana allergen extract, and the T3 test line coated with Artemisia annua allergen extract are arranged at intervals. By using nano - gold as the tracer substance and applying the biotin - avidin amplification reaction system, the sensitivity and specificity of the detection are improved, and the accurate detection of different species of Artemisia pollen allergen sources is realized. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] The present utility model will be further described in detail below in conjunction with the drawings and specific embodiments:
[0016] Figure 1 is the front view of a test strip for rapid detection of multiple Artemisia pollen allergens of the present utility model;
[0017] Figure 2 is the side - sectional view of a test strip for rapid detection of multiple Artemisia pollen allergens of the present utility model;
[0018] Figure 3 is the front view of the test strip body of the present utility model;
[0019] Figure 4 is the side - sectional view of the test strip body of the present utility model;
[0020] Figure 5 is Figure 4 the enlarged view of the structure at A in
[0021] Figure 6 is Figure 4 the enlarged view of the structure at B in
[0022] In the figure: 1 - sample pad, 2 - PVC bottom plate, 3 - secondary antibody pad, 4 - nano - gold pad, 5 - NC membrane, 51 - T1 test line, 52 - T2 test line, 53 - T3 test line, 54 - quality control line, 6 - absorbent pad, 7 - adhesive tape, 8 - outer shell, 81 - sample adding hole, 82 - observation window. DETAILED DESCRIPTION OF THE EMBODIMENTS
[0023] To make the objectives, technical solutions and advantages of the embodiments of the present utility model clearer, the technical solutions in the embodiments of the present utility model will be clearly and completely described below in conjunction with the embodiments of the present utility model. Obviously, the described embodiments are some but not all of the embodiments of the present utility model. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts fall within the scope of protection of the present utility model.
[0024] The following will further explain the present utility model with reference to the Figures 1-6 accompanying drawings:
[0025] A test strip for rapidly detecting multiple Artemisia pollen allergens, comprising a test strip body and an outer housing 8 arranged outside the test strip body. The test strip body includes a PVC bottom plate 2 and a solid-phase reaction membrane arranged on the PVC bottom plate 2. The solid-phase reaction membrane includes a secondary antibody pad 3, a nanogold pad 4 and an NC membrane 5. The middle of the top surface of the PVC bottom plate 2 is fixedly provided with the NC membrane 5. On the top surface of the NC membrane 5, a T1 detection line 51 coated with Artemisia argyi allergen extract, a T2 detection line 52 coated with Artemisia sieversiana allergen extract, a T3 detection line 53 coated with Artemisia annua allergen extract and a quality control line 54 coated with activated biotin are sequentially arranged at intervals from left to right. On the left side of the NC membrane 5, there are sequentially arranged a nanogold pad 4, a secondary antibody pad 3 and a sample pad 1 that overlap each other. The nanogold pad 4 is arranged on the left side of the NC membrane 5 and partially overlaps with the NC membrane 5. The secondary antibody pad 3 is arranged on the left side of the nanogold pad 4 and partially overlaps with the nanogold pad 4. The secondary antibody pad 3 is coated with a goat anti-human IgE secondary antibody labeled with activated biotin. The nanogold pad 4 is coated with nanogold particles labeled with streptavidin. The sample pad 1 is arranged on the left side of the secondary antibody pad 3 and partially overlaps with the secondary antibody pad 3. The NC membrane 5, the nanogold pad 4, the secondary antibody pad 3 and the sample pad 1 are fixedly connected by a tape 7. On the left side of the top surface of the outer housing 8, a sample adding hole 81 matching the sample pad 1 is opened. On the right side of the NC membrane 5, there is a water absorption pad 6 that partially overlaps with it. The NC membrane 5 and the water absorption pad 6 are fixedly connected by a tape 7. In the middle of the top surface of the outer housing 8, an observation window 82 matching the NC membrane 5 is opened.
[0026] In this embodiment, the width of the PVC bottom plate 2 is about 4 mm, the nano-gold pad 4 overlaps with the NC membrane 5 by about 1 mm, and the secondary antibody pad 3 overlaps with the nano-gold pad 4 by about 2 mm. Preferably, on the top surface of the outer housing 8, there are indication marks "S" for the sample adding hole 81, and indication marks "T1", "T2", "T3" and "C" for the T1 test line 51, T2 test line 52, T3 test line 53 and quality control line 54. The T1 test line 51 is used to detect whether the patient is allergic to Artemisia argyi, the T2 test line 52 is used to detect whether the patient is allergic to Artemisia sieversiana, the T3 test line 53 is used to detect whether the patient is allergic to Artemisia annua, and the quality control line 54 is used to detect whether the diluted serum of the patient has successively passed through the T1 test line 51, T2 test line 52, T3 test line 53 and reached the quality control line 54.
[0027] During use, place the outer housing 8 flat on the desktop, and drop 2 - 3 drops of the patient's diluted serum into the sample pad 1 from the sample adding hole 81. Under the adsorption of the absorbent pad 6, the diluted serum successively enters the secondary antibody pad 3, nano-gold pad 4 and NC membrane 5, re-dissolves and activates the biotin-labeled goat anti-human IgE secondary antibody and streptavidin-labeled nano-gold particles, and successively undergoes antigen-antibody reactions and biotin-avidin amplification reactions. Wait for 5 - 30 minutes for the reaction, and observe from the observation window 82 whether the T1 test line 51, T2 test line 52, T3 test line 53 and quality control line 54 on the NC membrane 5 turn red to judge the test result. The possible results are as follows:
[0028] 1. When the quality control line 54 turns red and the T1 test line 51, T2 test line 52, T3 test line 53 do not turn red, the result is negative, indicating that there are no specific IgE antibodies corresponding to Artemisia argyi, Artemisia sieversiana and Artemisia annua allergens in the patient's serum, indicating that the patient is not allergic to these three kinds of wormwoods.
[0029] 2. When the T1 test line 51 and quality control line 54 turn red and the T2 test line 52, T3 test line 53 do not turn red, the result is T1 positive, indicating that there is only a specific IgE antibody corresponding to the Artemisia argyi allergen in the patient's serum, indicating that the patient is only allergic to Artemisia argyi.
[0030] 3. When the T2 test line 52 and quality control line 54 turn red and the T1 test line 51, T3 test line 53 do not turn red, the result is T2 positive, indicating that there is only a specific IgE antibody corresponding to the Artemisia sieversiana allergen in the patient's serum, indicating that the patient is only allergic to Artemisia sieversiana.
[0031] 4. When the T3 test line 53 and quality control line 54 turn red and the T1 test line 51, T2 test line 52 do not turn red, the result is T3 positive, indicating that there is only a specific IgE antibody corresponding to the Artemisia annua allergen in the patient's serum, indicating that the patient is only allergic to Artemisia annua.
[0032] 5. When the T1 test line 51, T2 test line 52, and quality control line 54 turn red, and the T3 test line 53 does not turn red, the result is positive for T1 and T2, indicating that there are specific IgE antibodies corresponding to Artemisia argyi and Artemisia sieversiana allergens in the patient's serum, indicating that the patient is allergic to Artemisia argyi and Artemisia sieversiana.
[0033] 6. When the T1 test line 51, T3 test line 53, and quality control line 54 turn red, and the T2 test line 52 does not turn red, the result is positive for T1 and T3, indicating that there are specific IgE antibodies corresponding to Artemisia argyi and Artemisia annua allergens in the patient's serum, indicating that the patient is allergic to Artemisia argyi and Artemisia annua.
[0034] 7. When the T2 test line 52, T3 test line 53, and quality control line 54 turn red, and the T1 test line 51 does not turn red, the result is positive for T2 and T3, indicating that there are specific IgE antibodies corresponding to Artemisia sieversiana and Artemisia annua allergens in the patient's serum, indicating that the patient is allergic to Artemisia sieversiana and Artemisia annua.
[0035] 8. When the T1 test line 51, T2 test line 52, T3 test line 53, and quality control line 54 all turn red, the result is positive for T1, T2, and T3, indicating that there are specific IgE antibodies corresponding to Artemisia argyi, Artemisia sieversiana, and Artemisia annua allergens in the patient's serum, indicating that the patient is allergic to Artemisia argyi, Artemisia sieversiana, and Artemisia annua.
[0036] The present utility model adopts the nano - gold immunochromatographic assay method, simplifies the operation process, and only needs to add the specimen once to complete the experimental operation, avoiding complex steps such as adding reagents and washing multiple times, greatly reducing the operation difficulty. Using the nano - gold immunochromatographic assay method, the detection time is shortened to within 5 - 30 minutes to judge the result, and the detection speed is fast. On the NC membrane 5, the T1 test line 51 coated with Artemisia argyi allergen extract, the T2 test line 52 coated with Artemisia sieversiana allergen extract, and the T3 test line 53 coated with Artemisia annua allergen extract are arranged at intervals. By using nano - gold as the tracer substance and applying the biotin - avidin amplification reaction system, the sensitivity and specificity of the detection are improved, realizing the accurate detection of different species of Artemisia pollen allergen sources.
[0037] In summary, the present utility model is not limited to the above - mentioned specific embodiments. Those skilled in the art can make several changes and modifications on the premise of not departing from the spirit and scope of the present utility model. The protection scope of the present utility model shall be subject to the claims of the present utility model.
[0038] In the description of this patent, it should be understood that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing this patent and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this patent.
[0039] In the description of this patent, it should be noted that unless otherwise clearly specified and limited, the terms "installed", "connected", "joined", "set" should be understood in a broad sense. For example, it can be fixedly connected and set, or detachably connected and set, or integrally connected and set. For those of ordinary skill in the art, the specific meanings of the above terms in this patent can be understood according to specific circumstances.
Claims
1. A test strip for rapidly detecting multiple Artemisia pollen allergens, characterized in that, It includes a test strip body and an outer housing (8) arranged outside the test strip body. The test strip body includes a PVC bottom plate (2) and a solid-phase reaction membrane arranged on the PVC bottom plate (2). The solid-phase reaction membrane includes a secondary antibody pad (3), a nano-gold pad (4), and an NC membrane (5). The NC membrane (5) is fixedly arranged in the middle of the top surface of the PVC bottom plate (2). The top surface of the NC membrane (5) is successively and spacedly provided with a T1 test line (51), a T2 test line (52), a T3 test line (53), and a quality control line (54) from left to right. The left side of the NC membrane (5) is successively provided with an overlapping nano-gold pad (4), a secondary antibody pad (3), and a sample pad (1). The nano-gold pad (4) is arranged on the left side of the NC membrane (5) and partially overlaps with the NC membrane (5). The secondary antibody pad (3) is arranged on the left side of the nano-gold pad (4) and partially overlaps with the nano-gold pad (4). The sample pad (1) is arranged on the left side of the secondary antibody pad (3) and partially overlaps with the secondary antibody pad (3). The right side of the NC membrane (5) is provided with a water-absorbing pad (6) that partially overlaps with it.
2. The test strip for rapidly detecting multiple Artemisia pollen allergens according to claim 1, wherein The T1 test line (51) is coated with Artemisia argyi allergen extract, the T2 test line (52) is coated with Artemisia sieversiana allergen extract, the T3 test line (53) is coated with Artemisia annua allergen extract, and the quality control line (54) is coated with activated biotin.
3. The test strip for rapidly detecting multiple Artemisia pollen allergens according to claim 2, characterized in that, The secondary antibody pad (3) is coated with goat anti-human IgE secondary antibody labeled with activated biotin, the nano-gold pad (4) is coated with nano-gold particles labeled with streptavidin, and the NC membrane (5) is a nitrocellulose membrane.
4. A test strip for rapidly detecting multiple Artemisia pollen allergens as described in claim 1, characterized in that, The NC membrane (5), nano-gold pad (4), secondary antibody pad (3), and sample pad (1) are fixedly connected by a tape (7), and the NC membrane (5) and the water-absorbing pad (6) are also fixedly connected by a tape (7).
5. A test strip for rapidly detecting multiple Artemisia pollen allergens as described in claim 1, characterized in that, A sample addition hole (81) matching the sample pad (1) is opened on the left side of the top surface of the housing (8).
6. The test strip for rapid detection of multiple Artemisia pollen allergens according to claim 4, wherein An observation window (82) matching the NC membrane (5) is opened in the middle of the top surface of the housing (8).