Culture device for extracting stem cells from tissues

Abstract

The utility model discloses a culture device for extracting stem cells from tissues, which comprises a culture bin, a physical pressing auxiliary mechanism is mounted on the inner side of the culture bin and used for pressing the cell tissues, the physical pressing auxiliary mechanism comprises a pressing plate, and a height limiting column is mounted on the lower side of the pressing plate; a cover body is mounted at the upper end of the culture bin, the upper end of the cover body is open, and a limiting ring is correspondingly mounted at the opening of the upper end of the cover body and used for limiting and mounting a hydrophobic filter membrane; an auxiliary positioning mechanism is mounted on the lower side of the culture bin, the auxiliary positioning mechanism comprises a mounting plate, and positioning grids are arranged on the surface of the mounting plate in a criss-cross manner. The utility model belongs to the technical field of cell culture. The tissue culture device has the technical effects that the problem of small cell adherence quantity caused by tissue suspension in the tissue culture process can be solved, so that the waste of tissues is solved, and the utilization rate is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, and particularly relates to a culture device for extracting stem cells from tissues. Background Technique

[0002] When collecting umbilical cord mesenchymal stem cells from umbilical cord tissues, first cut the obtained neonatal umbilical cord tissues into 2-cm segments, wash the umbilical cord with PBS, cut the umbilical cord into 2-mm pieces after removing arteries and veins, attach the tissue blocks to a 6-well plate or culture flask at intervals of 5 mm, drop a special culture medium on them and place them in an incubator for culture. This method is the most common, but there are many problems, mainly including:

[0003] The problem of the adhesion stability of tissues, the pollution caused by insufficient sealing of the culture device, the low efficiency of cell harvesting, and the waste of tissues, etc. Among them, the suspension of tissues caused by unstable adhesion is the most likely problem to occur. Some researchers have also pre-coated biocompatible materials such as polylysine or gelatin on the bottom of the culture container to enhance the adhesion between tissues and the culture medium, thereby promoting the adhesion of cells. This method does help to improve the adhesion efficiency of tissues, but at the same time it also brings the problem of difficult cell digestion, which in turn affects the cell harvest, so it is not recommended to use.

[0004] In order to solve the above problems and balance the difficulties of tissue adhesion and cell digestion, we have designed an innovative culture device. Content of the Utility Model

[0005] Therefore, the utility model provides a culture device for extracting stem cells from tissues to solve the above problems in the prior art.

[0006] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0007] According to the first aspect of the utility model, a culture device for extracting stem cells from tissues includes:

[0008] A culture chamber, inside which a physical pressing assistance mechanism is installed for pressing cell tissues. Among them, the physical pressing assistance mechanism includes a pressing plate, and a height-limiting column is installed on the lower side of the pressing plate;

[0009] A cover body is installed at the upper end of the culture chamber. The upper end of the cover body is open, and a limiting ring is correspondingly installed at the opening of the upper end of the cover body. The longitudinal section of the limiting ring is in an "L" shape and is used for limiting and installing a hydrophobic filter membrane;

[0010] An auxiliary positioning mechanism is installed at the lower side of the culture chamber. Among them, the auxiliary positioning mechanism includes a mounting plate, and positioning grids are arranged vertically and horizontally on the surface of the mounting plate.

[0011] Furthermore, the height-limiting column is 1-2 mm higher than the thickness of the tissue.

[0012] Furthermore, arc-shaped groove structures are arranged inwardly at the four corners of the culture chamber.

[0013] Furthermore, limit blocks corresponding to the arc-shaped groove structures arranged at the four corners of the culture chamber are fixed to the outer ends of the mounting plates, and the limit blocks are engaged with the arc-shaped groove structures.

[0014] Furthermore, an auxiliary sealing ring is arranged on the outer surface of the culture chamber, and a groove structure for engaging with the cover body is formed on the inner side of the auxiliary sealing ring, and the groove structure on the inner side of the auxiliary sealing ring is arranged in a stepped manner.

[0015] Furthermore, a positioning iron block is installed inside the height-limiting column, and a magnet corresponding to the height-limiting column is also installed at the lower end of the culture chamber.

[0016] The utility model has the following advantages:

[0017] The utility model can solve the problem that tissue suspension occurs during tissue culture, resulting in a small amount of cell adhesion, thereby solving tissue waste and improving utilization rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 A perspective view of a culture device for extracting stem cells from tissues according to some embodiments of the utility model.

[0019] Figure 2 An exploded perspective view of a culture device for extracting stem cells from tissues according to some embodiments of the utility model.

[0020] Figure 3 An exploded perspective view of the connection between the magnet and the culture chamber of a culture device for extracting stem cells from tissues according to some embodiments of the utility model.

[0021] Figure 4 A front sectional view of a culture device for extracting stem cells from tissues according to some embodiments of the utility model.

[0022] Figure 5 For the utility model Figure 4 The enlarged schematic view of the structure at A.

[0023] Figure 6 For the utility model Figure 4 The enlarged schematic view of the structure at B.

[0024] In the figure: 1. Culture chamber; 2. Physical pressing assistance mechanism; 201. Pressing plate; 202. Height-limiting column; 203. Positioning iron block; 3. Cover body; 4. Auxiliary sealing ring; 5. Limiting ring; 6. Auxiliary positioning mechanism; 601. Mounting plate; 602. Limiting block; 603. Positioning grid; 7. Magnet. Detailed implementation manners

[0025] The following specific embodiments illustrate the implementation manners of the present utility model. Those skilled in the art can easily understand other advantages and effects of the present utility model from the content disclosed in this specification. Obviously, the described embodiments are part of the embodiments of the present utility model, rather than all of them. Based on the embodiments in the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0026] Embodiment 1

[0027] As Figures 1 to 6 shown, a culture device for extracting stem cells in tissues in the first aspect embodiment of the present utility model includes a culture chamber 1. A physical pressing assistance mechanism 2 is installed inside the culture chamber 1 for pressing cell tissues. Among them, the physical pressing assistance mechanism 2 includes a pressing plate 201. A height-limiting column 202 is installed on the lower side of the pressing plate 201. The height-limiting column 202 is 1-2 mm higher than the thickness of the cell tissues, aiming to prevent the cell tissues from floating during the culture process. At the same time, the non-full-surface pressing design helps to maintain the three-dimensional structure of the tissues.

[0028] A cover body 3 is installed at the upper end of the culture chamber 1. The upper end of the cover body 3 is open. A limiting ring 5 is correspondingly installed at the open upper end of the cover body 3. And the longitudinal section of the limiting ring 5 is in an "L" shape, which is used for limiting and installing a hydrophobic filter membrane to achieve aseptic gas exchange, effectively reducing the pollution risk, and at the same time ensuring the stability of the culture environment and the healthy growth of cell tissues.

[0029] An auxiliary positioning mechanism 6 is installed at the lower side of the culture chamber 1. Among them, the auxiliary positioning mechanism 6 includes a mounting plate 601, and positioning grids 603 are arranged vertically and horizontally on the surface of the mounting plate 601, which helps the user accurately lay the cell tissues flat to the designated position and ensure the uniformity of the culture process.

[0030] Specifically, by using the transparent visible material of the culture chamber 1, the positioning grid 603 on the bottom surface of the culture chamber 1 can be seen by the user. Thus, the cell tissues are evenly placed in the grids formed by the positioning grid 603. Then, the physical pressing assistance mechanism 2 is put in and the cell tissues are pressed to prevent the cell tissues from floating during the culture process. At the same time, the non-full-surface pressing design helps to maintain the three-dimensional structure of the tissues, thereby increasing the cell adhesion amount.

[0031] Example 2

[0032] As Figure 2 and Figure 3 shown, a culture device for extracting stem cells from tissues includes all the contents of Example 1. In addition, arc-shaped groove structures are arranged inward at the four corners of the culture chamber 1.

[0033] The technical effects achieved by the above embodiment are as follows: When different culture devices are placed together, it is convenient to take them from the gaps at the corners, and it is also convenient to install other devices without increasing the space occupation.

[0034] Example 3

[0035] As Figure 2 and Figure 3 shown, a culture device for extracting stem cells from tissues includes all the contents of Example 2. In addition, a limiting block 602 corresponding to the arc-shaped groove structures arranged at the four corners of the culture chamber 1 is fixed to the outer end of the mounting plate 601, and the limiting block 602 and the arc-shaped groove structure form a snap connection.

[0036] The technical effects achieved by the above embodiment are as follows: It can position the mounting plate 601 and prevent it from moving randomly.

[0037] Example 4

[0038] As Figure 1 and Figure 4 shown, a culture device for extracting stem cells from tissues includes all the contents of Example 3. In addition, an auxiliary sealing ring 4 is arranged on the outer surface of the culture chamber 1, and a groove structure for engaging with the cover body 3 is formed on the inner side of the auxiliary sealing ring 4, and the groove structure on the inner side of the auxiliary sealing ring 4 is arranged in a stepped shape.

[0039] The technical effects achieved by the above embodiment are as follows: Water can be filled in the auxiliary sealing ring 4 to compensate for the possible gaps between the cover body 3 and the auxiliary sealing ring 4, and prevent external pollution.

[0040] Example 5

[0041] As Figure 3 and Figure 5 shown, a culture device for extracting stem cells from tissues includes all the contents of Example 1. In addition, a positioning iron block 203 is installed inside the height-limiting column 202, and a magnet 7 corresponding to the height-limiting column 202 is also installed at the lower end of the culture chamber 1.

[0042] The technical effects achieved by the above embodiment are as follows: Utilizing the magnetic attraction effect between the positioning iron block 203 and the magnet 7, the height-limiting column 202 is kept in contact with the bottom surface of the culture chamber 1, so that the pressing plate 201 can always be in contact with the cell tissue.

Claims

1. A culture device for extracting stem cells from tissues, characterized in that, Comprising: A culture chamber (1), inside which a physical pressing assistance mechanism (2) is installed for pressing cell tissues. Among them, the physical pressing assistance mechanism (2) includes a pressing plate (201), and a height-limiting column (202) is installed on the lower side of the pressing plate (201); A cover body (3) is installed at the upper end of the culture chamber (1), the upper end of the cover body (3) is open, a limiting ring (5) is correspondingly installed at the opening of the upper end of the cover body (3), and the longitudinal section of the limiting ring (5) is in an "L" shape for limiting and installing a hydrophobic filter membrane; An auxiliary positioning mechanism (6) is installed on the lower side of the culture chamber (1). Among them, the auxiliary positioning mechanism (6) includes a mounting plate (601), and positioning grids (603) are arranged in a criss-cross manner on the surface of the mounting plate (601).

2. The culture device for extracting stem cells in tissues according to claim 1, characterized in that, The height-limiting column (202) is 1-2 mm higher than the thickness of the cell tissue.

3. The culture device for extracting stem cells in tissue according to claim 1, characterized in that, Arc-shaped groove structures are arranged inward at the four corners of the culture chamber (1).

4. A culture device for extracting stem cells in tissues according to claim 3, characterized in that, Limit blocks (602) corresponding to the arc-shaped groove structures arranged at the four corners of the culture chamber (1) are fixed at the outer ends of the mounting plate (601), and the limit blocks (602) are in snap connection with the arc-shaped groove structures.

5. The culture device for extracting stem cells in tissues according to claim 1, wherein, An auxiliary sealing ring (4) is arranged on the outer surface of the culture chamber (1), and a groove structure for engaging with the cover body (3) is opened on the inner side of the auxiliary sealing ring (4), and the groove structure on the inner side of the auxiliary sealing ring (4) is arranged in a stepped manner.

6. The culture device for extracting stem cells in a tissue according to claim 1, wherein A positioning iron block (203) is installed inside the height-limiting column (202), and a magnet (7) corresponding to the height-limiting column (202) is also installed at the lower end of the culture chamber (1).

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