Device for collecting root exudates at initial stage of plant growth

Abstract

A device for collecting root exudates in the initial stage of plant growth belongs to the technical field of collection of root exudates, and comprises a square vessel, a central incubator and a seed container which are overlapped together, and a liquid injection pipe is integrally formed on the side wall of the central incubator. In the operation process, different appropriate culture modes can be adopted for different growth stages of the plant from the seed germination stage, influences of various interference factors such as culture conditions and operation methods in the root exudate collection process are avoided, and meanwhile the function of observing the growth state of the plant root system is achieved. Plant root exudates at different parts in different stages can be collected, and comprehensiveness, accuracy and matching of collection of the root exudates in the initial growth stage of plants are guaranteed. And meanwhile, the device is designed into a simple detachable structure and has the characteristics of high operability and convenience in disassembly and maintenance.

Description

Technical Field

[0001] The utility model belongs to the technical field of the collection of root exudates, and particularly relates to a collection device for root exudates at the initial stage of plant growth. Background Technique

[0002] Root exudates are the medium for the exchange of substances, energy flow, and information transfer between plants and the surrounding environment, and are closely related to the growth regulation of plants. Collecting and studying the component characteristics of root exudates has important practical guiding significance for reasonably and effectively regulating the plant rhizosphere system, maximizing the ecological function of plant roots, improving the utilization efficiency of nutrient resources and crop productivity, and realizing the sustainable development of agricultural production.

[0003] Currently, the technical methods for collecting root exudates are mainly divided into soil culture method, sand culture method, and nutrient solution culture method (hydroponics method), and each method has its own advantages and disadvantages. The exudates collected by the soil culture method are relatively comprehensive, but the collection amount is small, the collection process is relatively complex, and the operation accuracy requirements are high; the sand culture method is relatively simple to operate, but it is subject to greater resistance during the growth of plant roots, and it cannot accurately reflect the secretion situation under natural growth conditions, and the processes of washing sand and scouring sand are cumbersome and easy to introduce impurities; the hydroponics method is simple and easy to operate, and it is easy to exclude the interference of foreign substances, but the plant roots are in a flooded state for a long time, which is easy to cause root hypoxia and rot, and there are great differences from the actual soil conditions. On the other hand, during the collection process of root exudates, the accuracy of collecting samples by the existing root exudate collection devices is also interfered by various factors, such as microbial contamination, impurities in the culture solution, etc. Especially for plants at the initial stage of growth after seed germination, the roots are fragile and grow fast, and it is necessary to consider minimizing the disturbance to the plant roots during the operation process, reducing the damage or stress caused to the roots, and avoiding affecting the normal growth and secretion function of plants. In order to reduce the interference factors in the plant culture process and improve the accuracy and operability of root exudate collection, a simple collection device and method for root exudates at the initial stage of plant growth are proposed. Summary of the Invention

[0004] Aiming at the deficiencies of the existing technology, the purpose of the utility model is to provide a collection device for root exudates at the initial stage of plant growth.

[0005] The technical solution adopted by the utility model is: a device and method for collecting root exudates in the initial stage of plant growth. The technical key points are as follows: it includes a square dish with an open top. A sealing port is provided at the bottom of the square dish, and a sealing plug is provided at the sealing port and is threadedly connected thereto. A cover plate with a central incubator is lapped on the square dish. The central incubator extends into the interior of the square dish, and injection tubes are integrally formed on the four side walls of the central incubator respectively. The upper ends of the injection tubes are plugged with injection syringes. The elbows of the injection tubes face the inner cavity of the central incubator and are blocked by rubber sealing plugs. A seed container is lapped at the opening at the top of the central incubator. The seed container has an upper edge that is slightly larger than the upper opening of the central incubator and is used for lapping on the central incubator. The bottom of the seed container is basin-shaped and sinks towards the interior of the central incubator, and the bottom of the seed container is a grid structure

[0006] In the above solution, the lengths of the injection tubes on the four outer walls of the central incubator are set according to the root length, so that the elbows at the bottom of the central incubator correspond to different collection parts of the roots.

[0007] In the above solution, the cover plate, the central incubator, the liquid injection tube, and the sealing plug are all made of quartz glass.

[0008] In the above solution, the seed container is made of PVC material.

[0009] The beneficial effects of the utility model are as follows: The device for collecting root exudates in the initial stage of plant growth of the utility model includes a square dish, a central incubator, and a seed container that are lapped together. Among them, injection tubes are integrally formed on the side walls of the central incubator. Considering the advantages and disadvantages of different cultivation and collection methods, different suitable cultivation methods can be adopted for different growth stages of plants starting from the seed germination stage during the operation process, avoiding the influence of various interference factors such as cultivation conditions and operation methods during the collection of root exudates. At the same time, it has the function of observing the growth state of plant roots, can collect root exudates of different parts at different stages, and ensures the comprehensiveness, accuracy, and matching of the collection of root exudates in the initial stage of plant growth. At the same time, the device is designed as a simple detachable structure, with the characteristics of strong operability, convenient disassembly and maintenance. Description of the Drawings

[0010] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required for use in the embodiments. Obviously, the drawings in the following description are only some embodiments of the present utility model. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0011] Figure 1 It is a schematic structural diagram of the device for collecting root exudates in the initial stage of plant growth in the embodiment of the present utility model;

[0012] Figure 2 Schematic diagram of the structure of the central incubator in the embodiment of the present utility model;

[0013] Figure 3 Schematic diagram of the structure of the seed container in the embodiment of the present utility model;

[0014] Explanation of the numbers in the figure: 1 square dish, 2 first sealing plug, 3 cover plate, 4 central incubator, 5 liquid injection tube, 6 rubber sealing plug, 7 injection syringe, 8 second sealing plug, 9 seed container. Specific implementation mode

[0015] To make the above objects, features, and advantages of the present utility model more obvious and understandable, the following combines the attached Figures 1 - 3 and specific implementation modes to further elaborate on the present utility model in detail.

[0016] The root exudate collection device in this embodiment uses a square dish 1 as the collection dish. The square dish 1 is a square structure with an open top. A circular sealing opening is provided at the center of the bottom of the square dish 1, and a first sealing plug 2 is installed at the circular sealing opening. The sealing method is screw rotation. The materials of the side wall, bottom, and sealing plug 2 of the square dish 1 are all quartz glass. Using quartz glass material does not cause impurity interference to the collected liquid, and at the same time meets the requirement of observing the root growth state during the plant cultivation and collection process. The structure of the culture dish 1 with an open top and a sealing opening at the bottom is convenient for the renewal and replacement of different forms of culture media.

[0017] It also includes a cover plate 3 with a size slightly larger than the upper edge of the collection dish. The cover plate 3 is used as a culture dish and can be directly mounted on the square dish 1 and taken on and off at any time. An upward-opening central incubator 4 is integrally formed on the cover plate 3. The four walls and the bottom of the central incubator 4 are connected to form a square structure and are located inside the square dish 1, which is convenient for direct taking and cleaning. A liquid injection tube 5 is provided on each of the four side walls of the central incubator 4. The liquid injection tube 5 and the central incubator 4 are integrally formed, and the liquid injection tubes 5 are arranged in sequence at different heights, that is, corresponding to different parts of the plant roots, for pouring nutrient solution or distilled water. A bend is provided below the liquid injection tube 5 and faces the inner cavity of the central incubator 4. The bend has a cylindrical liquid outlet head, and the diameter of the part from the bend to the liquid outlet head gradually decreases. A rubber sealing plug 6 is provided at the liquid outlet head. An injection pusher 7 made of PVC + rubber is provided at the upper end of the liquid injection tube 5, which is convenient for controlling the flushing speed and flow rate and ensuring that the root system is not damaged during the flushing process. A circular plugging port is provided at the center of the bottom of the central incubator 4, and a second sealing plug 8 is installed at the circular plugging port for releasing the culture medium, and the installation method is screw rotation. The collection dish 1 adopts a square appearance and a design with a sealing plug at the bottom, considering two different acquisition requirements for the collected liquid, namely direct pouring or bottom overflow. The cover plate 3, the central incubator 4 and the liquid injection tube 5 are made of quartz glass material, which is also to avoid impurity interference with the collected liquid and at the same time meet the requirement of observing the root growth state during the plant cultivation and collection process.

[0018] In this embodiment, a seed container 9 is used as a seed germination dish. The seed container 9 is made of PVC, with an upward-opening top, and the four sides and the bottom are sunken in a basin shape by 1 cm. The upper edge size is slightly larger than the edge of the top opening of the central incubator 4, and it can be directly mounted on the central incubator 4 and taken on and off at any time. The bottom is designed with a square grid to ensure the germination space of plant seeds and the nutrient supply of the required nutrient solution, and to avoid inhibiting the growth of the initial plant root system. The paving material (medical absorbent cotton, gauze, etc.) and the germination seed density can be selected according to needs.

[0019] In this embodiment, the connection method between the seed germination dish, the culture dish and the collection dish is by mounting, without adopting a fixed connection. Considering the high-frequency requirements for the renewal and collection of the culture medium during the initial stage of plant growth, the germination, culture and collection parts can be locally adjusted at any time, such as changing the plant species, renewing the culture medium, and obtaining the collected liquid. The independent design of each part ensures that it can be disassembled at any time, improving the simplicity of device operation, cleaning and maintenance.

[0020] A process for collecting root exudates during the initial stage of plant growth in this embodiment includes the following steps:

[0021] Lay two layers of medical absorbent cotton or gauze flat on the bottom grid of the seed container 9, evenly sprinkle the plant seeds on the cotton or gauze. After sealing the liquid injection nozzle of the central incubator 4, add hydroponic nutrient solution to ensure that the nutrient solution wets the cotton or gauze and always soaks the plant seeds. After the plant seeds germinate and grow roots, remove the cotton or gauze, take out the central incubator 4. According to the plant growth state and operation requirements, choose to replace the culture medium with nutrient solution (when there is a need to observe the root growth state), or culture soil, or a mixture of vermiculite and nutrient solution, and maintain the replacement frequency to keep the plant growing normally. At the collection stage, take out the seed germination dish, open the sealing port at the bottom of the central incubator 4, release the culture medium and wash the inside of the central incubator 4 with distilled water. Briefly rinse the roots with distilled water to remove the nutrient matrix and ensure that the roots are not damaged. Take out the rubber plug 6 at the liquid injection tube 5. Then, put the culture dish, seed germination dish and the plant back on the collection dish. According to the need, add distilled water to the different liquid injection tubes corresponding to different collection parts, push the injection syringe 7 to wash and collect the root exudates. Finally, take out the seed germination dish and the culture dish, and choose to directly pour the collection dish or open the plug 2 at the bottom of the collection dish to obtain the root exudate collection solution for subsequent operations.

[0022] The utility model is used to collect the root exudates of various parts at each stage in the initial stage of plant growth. The proposed device and collection method can maximize the avoidance of the influence of interference factors such as other impurities during the cultivation and operation processes, meet the need to observe the root growth state during the plant cultivation and collection processes, and at the same time meet the high-frequency requirements for the replacement of the culture medium and the collection of root exudates in the initial stage of plant growth. Each component of the device is independently detachable, ensuring the simplicity of the whole process of operation, cleaning and maintenance. The utility model ensures the convenience and accuracy of collecting root exudates in the initial stage of plant growth.

[0023] The above is only the specific implementation manner of the utility model, but the protection scope of the utility model is not limited thereto. Any person skilled in the art within the technical scope disclosed by the utility model can easily think of changes or substitutions, which should all be covered within the protection scope of the utility model. Therefore, the protection scope of the utility model should be subject to the protection scope of the claimed rights.

Claims

1. A device for collecting root exudates during the initial growth stage of plants, characterized in that, It includes a square dish with an open top. A sealing opening is provided at the bottom of the square dish, and a sealing plug is provided at the sealing opening and is threadedly connected thereto; A cover plate with a central incubator is lapped on the square dish. The central incubator extends into the interior of the square dish, and injection tubes are integrally formed on the four side walls of the central incubator respectively. An injection pusher is inserted into the upper end of the injection tube, and the elbow of the injection tube faces the inner cavity of the central incubator and is blocked by a rubber sealing plug; A seed container is lapped at the top opening of the central incubator. The seed container has an upper edge that is slightly larger than the upper opening of the central incubator and is used for lapping on the central incubator. The bottom of the seed container sinks in a basin shape towards the inside of the central incubator, and the bottom of the seed container is a grid structure.

2. The root exudate collection device for the initial growth stage of plants according to claim 1, characterized in that, The lengths of the injection tubes located on the four outer walls of the central incubator are set according to the root length, so that the elbows located at the bottom of the central incubator correspond to different collection parts of the roots.

3. The root exudate collection device in the initial stage of plant growth according to claim 1 or 2, characterized in that, The cover plate, the central incubator, the liquid injection tube, and the sealing plug are all made of quartz glass.

4. The root exudate collection device for the initial growth stage of plants according to claim 3, characterized in that , The seed container is made of PVC material.

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