Wet box with dispersive and cyclic heating function

Abstract

The utility model relates to the technical field of immunohistochemistry, in particular to a disperse circulation heating wet box which comprises an antigen shell, a glass slide and a heat circulation system, the antigen shell comprises a shell body, an inlet and outlet, a lifting plate, a receding opening, a fixing part, a closing plate and a supporting plate, the shell body is located on a supporting frame, the inlet and outlet are located in the front end plate face of the shell body, and the lifting plate is located on the back end plate face of the shell body. The sealing plate is located in the shell where the inlet and outlet is located, the lifting plate is located on one side of the sealing plate, the receding opening is located above the lifting plate, the fixing part is located on the side edge of the upper end of the receding opening, and the supporting plate is located below the glass slide; comprising a heating part, a flow dividing part, a temperature rising part, a backflow part, a communicating part, a motor pump, a main flow pipe, a supporting shaft, a rotating fan blade, a heating assembly and a stirring fan blade, and through the design of a heating liquid circulation pipeline in the heating part, the heating liquid circulation time is shortened, and the heat loss of the heating liquid is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of immunohistochemistry, in particular to a wet box with dispersed circulating heating. Background Art

[0002] Immunohistochemistry (IHC) is a commonly used technique for cell and histological detection. It utilizes the principle of specific antigen-antibody binding, labels or links dyes to antibodies, and observes the staining results through a microscope to localize and quantitatively analyze specific antigens in cells or tissues. In immunohistochemistry, antigen retrieval is a crucial step, which can enhance the efficiency and sensitivity of antigen-antibody binding and improve the staining effect. Antigen retrieval usually uses heat treatment or enzyme treatment methods, among which heat treatment is the most commonly used method. In the heat treatment antigen retrieval method, samples are usually placed in a buffer with a moderate pH value and then heated at a high temperature to expose the antigens in the tissue. However, if the heating is uneven during the heating process, it may lead to poor antigen retrieval effect, thus affecting the staining effect. Therefore, appropriate measures need to be taken to ensure uniform heating during the heating process, thereby improving the antigen retrieval effect. For example, methods such as microwave, steam, or water bath heating can be used to ensure uniform heating; the samples can be cut into uniform thickness to ensure uniform heating, and the heating time and temperature can be appropriately controlled to avoid overheating or local antigen death. Summary of the Invention

[0003] Therefore, the utility model is a wet box with dispersed circulating heating, which heats the antigens on the glass slide through a set heat circulation device and sets the heating path to reduce the loss of heat during the movement of the heat flow. The utility model achieves the above object through the following technical solutions:

[0004] A wet box with dispersed circulating heating, comprising: an antigen housing, a glass slide, and a heat circulation system. The antigen housing includes: a housing, an inlet and outlet, a lifting plate, a relief opening, a fixing part, a closing plate, and a support plate. The housing is located on a support frame. The inlet and outlet are located on the front end surface of the housing for the entry and exit of the glass slide. The closing plate is located inside the housing where the inlet and outlet are located. The lifting plate is located on one side of the closing plate. The relief opening is located above the lifting plate. The fixing part is located on the upper side edge of the relief opening. The support plate is located below the glass slide. The heat circulation system includes: a heating part, a shunt part, a temperature rising part, a reflux part, a connecting part, a motor pump, a main flow pipe, a support shaft, a rotating fan blade, a heating component, and a stirring fan blade.

[0005] Preferably, the housing is used to support and fix the inlet and outlet, the lifting plate, the relief opening, the fixing part, the closing plate, and the support plate.

[0006] Preferably, the lifting plate has an L-shaped plate surface. Holes are provided on the vertical plate surface for fixing with the cross bar on the fixing part. A spring and a cross bar are arranged inside the fixing part.

[0007] Preferably, the heating part is located below the glass slide. The heating part has three layers inside and outside, presenting an annular hollow shape, and is internally connected to the shunt part and the main flow pipe respectively.

[0008] Preferably, the main flow pipe is located below the center of the heating part and is connected through it. The motor pump is located below the main flow pipe. The upper end of the motor pump is connected to the main flow pipe, and the lower end is connected to the temperature rising part.

[0009] Preferably, the heating component is located in the outer ring space of the temperature rising part. The heating component is in a spiral shape. The stirring fan blade is located at the connection between the outer ring space and the inner ring space of the temperature rising part. The fan blades of the stirring fan blade are divided into front and back sides, one side is arc-shaped, and the other side is flat.

[0010] Preferably, the rotating fan blade is located on the support shaft. The rotating fan blade is divided into three groups up and down and is rotationally connected to the support shaft. The shape of the rotating fan blade is the same as that of the stirring fan blade. The support shaft is located below the top plate surface of the heating part. The connecting part is located between the main flow pipe and the shunt part. The shunt part is located outside the heating part. The return part is located below the shunt part. The shunt part is divided into two groups of annular plates. The inside of the annular plates is hollow, and the lower end is connected to two heating spaces outside the heating part, and the upper end is connected to the connecting part.

[0011] Advantages of the utility model:

[0012] 1. By designing the pipeline for the heating liquid to flow inside the heating part, the utility model designs a three-layer flow pipeline with the same path inside, outside and in the middle. Compared with the traditional heating path, through layering and pipelines of the same length, the flow time of the heating liquid is reduced, and the heat loss of the heating liquid is reduced.

[0013] 2. The utility model heats the antigen on the glass slide through the set heat circulation device, reduces heat loss, and at the same time drives the stirring fan blade for heat homogenization of the heating liquid by the heating flow driven by the water pump, avoiding uneven heating caused by uneven heat flow temperature due to heat dissipation, resulting in the death of the final antigen cells and affecting the experiment. Description of the drawings

[0014] Figure 1 It is the front view of the utility model.

[0015] Figure 2 It is the rear view of the utility model.

[0016] Figure 3 It is the Figure 1 -A enlarged view of the utility model.

[0017] Figure 4 This is a schematic diagram of the components of the present utility model.

[0018] Figure 5 This is a bottom view of the present utility model.

[0019] Figure 6 This is a front view of the heat circulation system of the present utility model.

[0020] Figure 7 This is a bottom view of the heat circulation system of the present utility model.

[0021] Figure 8 This is a cross-sectional view of the heat circulation system of the present utility model.

[0022] Figure 9 This is a schematic diagram of the structure of the flow splitting part of the present utility model.

[0023] Figure 10 This is a schematic diagram of the structure of the stirring fan blade of the present utility model.

[0024] Explanation of reference numerals:

[0025] 1. Antigen housing; 11. Housing; 12. Inlet and outlet; 13. Lifting plate; 14. Relief opening; 15. Fixing part; 16. Sealing plate; 17. Support plate; 2. Slide; 3. Heat circulation system; 31. Heating part; 32. Flow splitting part; 34. Heating-up part; 35. Return part; 36. Connecting part; 37. Motor pump; 38. Main pipe; 39. Support shaft; 310. Rotating fan blade; 311. Heating component; 312. Stirring fan blade. Detailed implementation manners

[0026] Preferred embodiments of the present utility model will be described in detail with reference to the accompanying drawings. It is easy for those with ordinary skills in the art of the present utility model to implement these embodiments. However, the present utility model can also be implemented in various different forms. Therefore, the present utility model is not limited to the embodiments described below. Additionally, in order to describe the present utility model more clearly, components not connected to the present utility model will be omitted from the drawings.

[0027] As Figure 1 shown, a wet box with dispersed circulation heating includes: an antigen housing 1, a slide 2, and a heat circulation system 3;

[0028] As Figure 2 shown, the antigen housing 1 includes: a housing 11, an inlet and outlet 12, a lifting plate 13, a relief opening 14, a fixing part 15, a sealing plate 16, and a support plate 17;

[0029] The housing 11 is located on the support frame and is used to support and fix devices such as the inlet / outlet 12, the lifting plate 13, the relief opening 14, the fixing part 15, the closing plate 16, and the support plate 17;

[0030] The inlet / outlet 12 is located on the front end face of the housing 11 and is used for the entry and exit of the slide 2;

[0031] The closing plate 16 is located inside the housing 11 where the inlet / outlet 12 is located and is used to eliminate the influence of the opening of the inlet / outlet 12 on the internal sealing of the housing 11;

[0032] The lifting plate 13 is located on one side of the closing plate 16. The lifting plate 13 has an L-shaped plate surface. Holes are provided on the vertical plate surface for mutual fixation with the cross bar on the fixing part 15, and are used to lift the closing plate 16 through the lifting plate 13;

[0033] The relief opening 14 is located above the lifting plate 13 and is also located on the housing 11, and is used to provide a relief space for the lifting of the lifting plate 13;

[0034] The fixing part 15 is located at the upper end side of the relief opening 14. A spring and a cross bar are provided inside. When the lifting plate 13 is lifted to the top of the relief opening 14, the cross bar is pulled to align the cross bar with the hole on the lifting plate 13, and then the cross bar is released to enter the inside of the lifting plate 13 to complete the fixation, so that the operator can more conveniently place the slide 2;

[0035] The support plate 17 is located below the slide 2 and is used to support the slide 2;

[0036] As Figure 2 shown, the thermal cycling system 3 includes: a heating part 31, a shunt part 32, a temperature rising part 34, a reflux part 35, a connecting part 36, a motor pump 37, a main flow pipe 38, a support shaft 39, a rotating fan blade 310, a heating component 311, and an agitating fan blade 312;

[0037] The heating part 31 is located below the slide 2. The heating part 31 has three layers inside and outside and is in the shape of a ring with a hollow inside. The inside is respectively connected to the shunt part 32 and the main flow pipe 38, and is used to push and replace the heated heating liquid through the shunt part 32 and the main flow pipe 38;

[0038] The main flow pipe 38 is located below the center of the heating part 31 and is connected to each other through penetration. At the same time, the main flow pipe 38 is connected to the inner ring of the heating part 31 in a ring shape with a hollow. The main flow pipe 38 is also connected to the shunt part 32, and is used to supply fresh heating liquid to the heating part 31 and the shunt part 32;

[0039] The motor pump 37 is located below the main flow pipe 38. The upper end of the motor pump 37 is connected to the main flow pipe 38, and the lower end is connected to the temperature rising part 34, and is used to conduct the temperature rising part 34 into the main flow pipe 38;

[0040] The heating-up part 34 is located below the motor pump 37. The heating-up part 34 is cylindrical in shape, with an inner and outer two-layer circular ring space that is interconnected and is the area for accommodating the heating liquid for heating;

[0041] The heating component 311 is located in the outer circular ring space of the heating-up part 34. The heating component 311 is spiral in shape, has a large contact area with water, and a fast heating speed, and can better ensure the uniformity of the temperature of the heating liquid, and is used for heating the heating liquid;

[0042] The stirring fan blade 312 is located at the connection between the outer space and the inner space of the heating-up part 34. Since the heating liquid is driven by the motor pump 37, the heating liquid outside the heating part 31 is poured into the inner circle. During the pouring process, it flows through the stirring fan blade 312 from bottom to top. The fan blades of the stirring fan blade 312 are divided into front and back sides. One side is arc-shaped and the other side is flat. The water flow velocities on both sides are different, resulting in a flow velocity difference. Therefore, the pressure difference drives the stirring fan blade 312 to rotate, and mixes and stirs the heated flow that has completed heating. Since the distances between the heating liquid outside the heating part 31 and the heating component 311 are different, a situation of uneven heat conduction occurs. Therefore, through the stirring of the stirring fan blade 312, the temperature of the heated liquid that has completed heating is equalized;

[0043] The rotating fan blade 310 is located on the support shaft 39. The rotating fan blade 310 is divided into three groups up and down and is rotatably connected to the support shaft 39. The shape of the rotating fan blade 310 is the same as that of the stirring fan blade 312 and has the same function, and can stir the heating liquid to achieve the purpose of equalizing the temperature of the heating liquid. At the same time, the rotating fan blades 310 are respectively distributed at the through positions of the main flow pipe 38 and the heating part 31, and at the through positions of the main flow pipe 38 and the flow dividing part 32, and are used for equalizing the temperature of the heating liquid entering the three heating spaces of the heating part 31;

[0044] The support shaft 39 is located below the top plate surface of the heating part 31 and is used for supporting the rotating fan blade 310;

[0045] The connecting part 36 is located between the main flow pipe 38 and the flow dividing part 32 and is used for connecting the main flow pipe 38 and the flow dividing part 32;

[0046] The flow dividing part 32 is located on the outer circle of the heating part 31 and is interconnected. The flow dividing part 32 is divided into two groups of annular ring plates. The inside of the ring plates is hollow. The lower end is connected to the two heating spaces on the outer circle of the heating part 31, and the upper end is connected to the connecting part 36, and is used for conducting the heating liquid into the heating part 31;

[0047] The reflux part 35 is located below the three-layer heating space of the shunt part 32 and is interconnected. It is used to conduct the heated liquid that has completed heating to enter the outer circle of the temperature-raising part 34 through the reflux part 35 for reheating and then reuse.

[0048] The working principle of the present utility model:

[0049] Drive the external power supply to energize the heating component 311, and then drive the motor pump 37 to operate. Heat the heating liquid through the heating component 311. Then, driven by the motor pump 37, the heating liquid in the outer circle of the heating part 31 enters the inner circle and flows upward through the stirring fan blade 312. One end of the front and rear ends of the fan blade of the stirring fan blade 312 is arc-shaped and the other end is flat. The water flow velocities on both sides are different, resulting in a flow velocity difference. Therefore, the pressure difference drives the stirring fan blade 312 to rotate, and the heated flow that has completed heating is mixed and stirred. Since the distances of the heating liquid in the outer circle of the heating part 31 from the heating component 311 are different, a situation of uneven heat conduction occurs. Therefore, through the stirring of the stirring fan blade 312, the temperature of the heated liquid that has completed heating is equalized. Then, it enters the main flow pipe 38 from the inner circle of the heating part 31, and then enters the shunt part 32 through the communication part 36 respectively through the main flow pipe 38. At the same time, when the heating liquid enters the inner circle of the heating part 31 at the upper end of the main flow pipe 38, and when it enters the heating part 31, the heating liquid flow drives the rotating fan blade 310 to rotate to equalize the temperature of the heating liquid. Then, the heating liquid is injected into the three-section heating space inside the heating part 31 through the main flow pipe 38 and the shunt part 32 to complete the filling inside the heating part 31. Then, the heated liquid that has completed heating is reheated through the reflux part 35 to complete a heating cycle.

Claims

1. A wet box with decentralized circulating heating, comprising: Antigen housing (1), glass slide (2), thermal cycling system (3); characterized in that: the antigen housing (1) includes: a housing (11), an inlet / outlet (12), a lifting plate (13), a relief opening (14), a fixing portion (15), a closing plate (16), a support plate (17), the housing (11) is located on a support frame, the inlet / outlet (12) is located on the front end face of the housing (11) for the glass slide (2) to enter and exit, the closing plate (16) is located inside the housing (11) where the inlet / outlet (12) is located, the lifting plate (13) is located on one side of the closing plate (16), the relief opening (14) is located above the lifting plate (13), the fixing portion (15) is located at the upper side edge of the relief opening (14), the support plate (17) is located below the glass slide (2), and the thermal cycling system (3) includes: a heating portion (31), a flow splitting portion (32), a temperature rising portion (34), a return portion (35), a connecting portion (36), a motor pump (37), a main flow pipe (38), a support shaft (39), a rotating fan blade (310), a heating component (311), and a stirring fan blade (312).

2. The wet box for decentralized circulating heating according to claim 1, wherein: The housing (11) is used to support and fix the inlet / outlet (12), the lifting plate (13), the relief opening (14), the fixing portion (15), the closing plate (16), and the support plate (17).

3. The wet box with decentralized circulating heating according to claim 1, wherein: The lifting plate (13) has an L-shaped plate surface. Holes are provided on the vertical plate surface for mutual fixation with the cross bar on the fixing portion (15). A spring and a cross bar are provided inside the fixing portion (15).

4. A wet box with decentralized circulating heating according to claim 1, characterized in that: The heating portion (31) is located below the glass slide (2). The heating portion (31) has three layers inside and outside, and is in the shape of an annular hollow, and is internally connected to the flow splitting portion (32) and the main flow pipe (38) respectively.

5. A wet box with decentralized circulating heating according to claim 1, characterized in that: The main flow pipe (38) is located below the center of the heating portion (31) and is connected to it through penetration. The motor pump (37) is located below the main flow pipe (38). The upper end of the motor pump (37) is connected to the main flow pipe (38), and the lower end is connected to the temperature rising portion (34).

6. The wet box with decentralized circulating heating according to claim 1, characterized in that: The heating component (311) is located in the outer ring space of the temperature rising portion (34). The heating component (311) is in the shape of a spiral. The stirring fan blade (312) is located at the connection between the outer ring space and the inner ring space of the temperature rising portion (34). The fan blades of the stirring fan blade (312) are divided into front and back sides, one side is arc-shaped, and the other side is flat-shaped.

7. A wet box with decentralized circulating heating according to claim 1, characterized in that: The rotating fan blade (310) is located on the support shaft (39). The rotating fan blade (310) is divided into three groups up and down and is rotationally connected to the support shaft (39). The shape of the rotating fan blade (310) is the same as that of the stirring fan blade (312). The support shaft (39) is located below the top plate surface of the heating portion (31). The connecting portion (36) is located between the main flow pipe (38) and the flow splitting portion (32). The flow splitting portion (32) is located outside the heating portion (31). The return portion (35) is located below the flow splitting portion (32).

8. A wet box for decentralized cyclic heating according to claim 7, characterized in that: The flow splitting portion (32) is divided into two groups of annular ring plates. The inside of the ring plates is hollow. The lower end is connected to two heating spaces outside the heating portion (31), and the upper end is connected to the connecting portion (36).

Images