DNA synthesizer with ammonolysis assembly

Abstract

The utility model is applicable to the technical field, and provides a DNA synthesizer with an ammonolysis assembly, which comprises a DNA synthesizer body, the bottom of the inner cavity of the DNA synthesizer body is provided with a cavity, the front end of the cavity is provided with two groups of movably arranged front cover plates, the cavity is internally provided with a moving assembly, the moving assembly is provided with a connecting piece, and the connecting piece is provided with an ammonolysis assembly. A mounting plate is fixedly connected to the connecting piece, a mounting assembly is fixedly arranged on the top of the mounting plate, a cavity body is arranged on the mounting assembly, and two sets of fixing mechanisms are symmetrically arranged on the two sides of the mounting assembly so that the cavity body can be conveniently limited and fixed; a synthesis assembly, a waste discharge assembly and an ammonolysis assembly are mounted on the upper surface of the DNA synthesizer body, and the synthesis assembly, the waste discharge assembly and the ammonolysis assembly are communicated with the chamber main body; according to the utility model, the mounting assembly is matched with the fixing mechanism, and the chamber main body is taken out from the mounting assembly, so that the chamber main body and the mounting plate can be conveniently disassembled and assembled.

Description

Technical Field

[0001] The utility model belongs to the technical field of DNA synthesizers, and particularly relates to a DNA synthesizer with an ammonolysis component. Background Art

[0002] A DNA synthesizer is an automated instrument for synthesizing oligonucleotides structurally similar to DNA or RNA. An industrial DNA synthesizer mainly refers to a DNA synthesizer with a large synthesis throughput and a single-column synthesis product of 10 - 1000 nmol, as well as a large-scale synthesis type synthesizer; it is mainly used for synthesizing DNA molecules. The DNA synthesizer has the characteristics of high efficiency, precision, repeatability, etc., and is widely used in the fields of genetic engineering, drug research and development, biomedicine, etc.

[0003] Most of the existing ammonolyzers are independent of the DNA synthesizer. On the one hand, the ammonolyzers exist in the form of separate instruments. For ammonolysis, an ammonolyzer needs to be used, and the synthesis plate cannot directly react with the DNA synthesizer, which is troublesome to use, has low working efficiency, and the synthesis plate in the chamber is not convenient to disassemble and assemble, making it inconvenient for subsequent replacement. Summary of the Utility Model

[0004] The utility model provides a DNA synthesizer with an ammonolysis component, aiming to solve the problems that an ammonolyzer needs to be used for ammonolysis, the synthesis plate cannot directly react with the DNA synthesizer, it is troublesome to use, has low working efficiency, and the synthesis plate in the chamber is not convenient to disassemble and assemble.

[0005] The utility model is realized as follows: A DNA synthesizer with an ammonolysis component includes a DNA synthesizer body. A cavity is opened at the bottom of the inner cavity of the DNA synthesizer body. Two groups of movably arranged front covers are installed at the front end of the cavity. A moving component is arranged in the cavity, and a connecting piece is arranged on the moving component. An installation plate is fixedly connected to the connecting piece. An installation component is fixedly arranged on the top of the installation plate. A chamber body is arranged on the installation component. Two groups of fixing mechanisms are symmetrically arranged on both sides of the installation component to facilitate limiting and fixing the chamber body. A synthesis component, a waste discharge component, and an ammonolysis component are installed on the upper surface of the DNA synthesizer body, and the synthesis component, the waste discharge component, and the ammonolysis component are communicated with the chamber body;

[0006] The installation component includes sliders fixedly connected to both sides of the chamber body, and connecting plates fixedly arranged on the installation plate. Installation grooves are opened in the connecting plates. Chute grooves are opened at the front and rear ends inside the installation grooves, and the chamber body is slidably connected to the chute grooves through the sliders on both sides. Fixing grooves are opened at the front, rear, left, and right ends of the sliders.

[0007] Preferably, the ammonolysis component includes a sealing member, and an air inlet and an exhaust port are arranged on the sealing member.

[0008] Preferably, the ammonolysis component further includes a temperature monitoring component fixedly connected inside the chamber body.

[0009] Preferably, a heating component is provided on the outer wall of the chamber body, and the heating component can be a heating sheet.

[0010] Preferably, the fixing mechanism includes mounting blocks fixedly arranged at the front and rear ends on the left and right sides of the connecting plate. A connecting rod that cooperates with the fixing groove is slidably connected inside the mounting block. One end of the connecting rod is fixedly connected to a support block, and a spring is arranged on the outer side of the connecting rod.

[0011] Preferably, one end of the spring is fixedly connected to one side of the mounting block, the other end of the spring is fixedly connected to one side of the support block, and a handle is fixedly connected to one side of the support block.

[0012] Preferably, the mounting block is L-shaped, the fixing groove is adapted to the connecting rod, and the fixing groove is movably connected to the connecting rod.

[0013] Compared with the prior art, the embodiments of the present application mainly have the following beneficial effects:

[0014] 1. By providing a mounting component in cooperation with a fixing mechanism, taking out the chamber body from the mounting component can facilitate the disassembly and assembly of the chamber body and the mounting plate, facilitate the replacement of the composite board inside the chamber body, and there is an ammonolysis component provided in the device, so that the subsequent ammonolysis reaction can be carried out without taking out the composite board. By pulling the handle, the support block drives the connecting rod to move. When the support block moves, the spring is stretched, so that the connecting rod is pulled out of the fixing groove, and then the chamber body is taken out from the mounting component, which can facilitate the disassembly and assembly of the chamber body and the mounting plate and facilitate the replacement of the composite board inside the chamber body; since the ammonolysis component includes a provided seal, a sealed structure is formed between the seal and the cavity, preparing for the subsequent ammonolysis reaction. Then open the air inlet and close the exhaust port, inject ammonia gas into the chamber body, and when the air pressure inside the chamber body reaches the required pressure, close the air inlet. Then heat the chamber body under the action of the heating component. When the temperature inside the chamber body shown by the temperature monitoring component reaches the required temperature, stop heating. Let it stand for a period of time. After the ammonolysis is complete, open the exhaust port to recover the residual gas that did not participate in the reaction in the instrument, and the purpose of the subsequent ammonolysis reaction can be carried out without taking out the composite board. Setting the above structure can not only reduce the manual operation steps but also reduce the manufacturing cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 is the overall three-dimensional view of the present invention;

[0016] Figure 2 is the overall structural schematic diagram of the present invention;

[0017] Figure 3 is the Figure 2 enlarged structural schematic diagram of part A in

[0018] Figure 4 is the Figure 3 enlarged structural schematic diagram of part B in

[0019] In the figure: 1. DNA synthesizer body; 2. cavity; 3. chamber main body; 4. rotor; 5. mounting plate; 6. mounting assembly; 601. chute; 602. mounting groove; 603. slider; 604. fixing groove; 605. connecting plate; 7. fixing mechanism; 701. mounting block; 702. connecting rod; 703. spring; 704. support block; 705. handle; 8. air inlet; 9. air outlet; 10. connecting piece; 11. heating assembly; 12. temperature monitoring component; 13. seal. Specific embodiments

[0020] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field to which this application belongs; the terms used in the description of this application in the specification are only for the purpose of describing specific embodiments and are not intended to limit this application; the terms "including" and "having" and any variations thereof in the specification and claims of this application and the above drawings are intended to cover non-exclusive inclusion. The terms "first", "second", etc. in the specification and claims of this application or the above drawings are used to distinguish different objects and not to describe a specific order.

[0021] Reference to "embodiment" herein means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of this application. The phrase appears in various places in the specification and does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment mutually exclusive with other embodiments. Those skilled in the art will explicitly and implicitly understand that the embodiments described herein can be combined with other embodiments.

[0022] An embodiment of the present invention provides a DNA synthesizer with an ammonolysis component, as Figures 1-4As shown in the figure, it includes the DNA synthesizer body 1. A cavity 2 is opened at the bottom of the inner cavity of the DNA synthesizer body 1. Two groups of front covers are movably installed at the front end of the cavity 2. A moving component is arranged in the cavity 2. A connecting piece 10 is arranged on the moving component. An installation plate 5 is fixedly connected to the connecting piece 10. An installation component 6 is fixedly arranged on the top of the installation plate 5. A chamber body 3 is arranged on the installation component 6. Two groups of fixing mechanisms 7 are symmetrically arranged on both sides of the installation component 6 to facilitate the limit fixation of the chamber body 3. A synthesis component, a waste discharge component and an ammonolysis component are installed on the upper surface of the DNA synthesizer body 1. The synthesis component, the waste discharge component and the ammonolysis component are communicated with the chamber body 3;

[0023] The installation component 6 includes sliders 603 fixedly connected to both sides of the chamber body 3, and a connecting plate 605 fixedly arranged on the installation plate 5. An installation groove 602 is opened in the connecting plate 605. Chute 601 is opened at the front and rear ends inside the installation groove 602. The chamber body 3 is slidably connected in the chute 601 through the sliders 603 on both sides. Fixing grooves 604 are opened at the front and rear ends on the left and right sides of the slider 603.

[0024] It should be noted that since most of the existing ammonolysis instruments are independent of the DNA synthesizer, on the one hand, the ammonolysis instrument exists in the form of a separate instrument. Ammonolysis requires the use of the ammonolysis instrument, and the synthesis plate cannot directly react with the DNA synthesizer, which is troublesome to use, has low work efficiency, and the synthesis plate in the chamber is not convenient to disassemble and assemble, and is not convenient for subsequent replacement. Therefore, in order to solve the problems that the existing ammonolysis requires the use of the ammonolysis instrument, the synthesis plate cannot directly react with the DNA synthesizer, it is troublesome to use, has low work efficiency, and the synthesis plate in the chamber is not convenient to disassemble and assemble, this solution is to set up the installation component 6 in cooperation with the fixing mechanism 7 to take out the chamber body 3 from the installation component 6, which can facilitate the disassembly and assembly of the chamber body 3 and the installation plate 5, facilitate the replacement of the synthesis plate in the chamber body 3, and there is an ammonolysis component arranged in the device, so that the subsequent ammonolysis reaction can be carried out without taking out the synthesis plate.

[0025] Specifically, in this embodiment, this solution mainly sets up an installation component 6 in cooperation with a fixing mechanism 7. Taking out the chamber body 3 from the installation component 6 can facilitate the disassembly and assembly of the chamber body 3 and the installation plate 5, and is convenient for replacing the synthesis plate in the chamber body 3. And an ammonolysis component is provided in the device, so that the subsequent ammonolysis reaction can be carried out without taking out the synthesis plate. By pulling the handle 705, the support block 704 drives the connecting rod 702 to move. When the support block 704 moves, the spring 703 is stretched, so that the pulling connecting rod 702 disengages from the fixing groove 604, and then the chamber body 3 is taken out from the installation component 6, which can facilitate the disassembly and assembly of the chamber body 3 and the installation plate 5, and is convenient for replacing the synthesis plate in the chamber body 3; the ammonolysis component includes a provided seal, so that a sealed structure is formed between the seal and the cavity 2 to prepare for the subsequent ammonolysis reaction. Then the air inlet 8 is opened, the exhaust port 9 is closed, and ammonia gas is injected into the chamber body 3. At the same time, when the air pressure in the chamber body 3 reaches the required pressure, the air inlet 8 is closed. Then, under the action of the heating component 11, the chamber body 3 is heated. When the temperature in the chamber body 3 displayed by the temperature monitoring component 12 reaches the required temperature, the heating is stopped. After standing for a period of time, after the ammonolysis is complete, the exhaust port 9 is opened to recover the residual gas that did not participate in the reaction in the instrument, and the purpose of the subsequent ammonolysis reaction can be carried out without taking out the synthesis plate. Setting the above structure can not only reduce the manual operation steps, but also reduce the manufacturing cost.

[0026] In a further preferred embodiment of the present utility model, as Figures 2-4 shown, the ammonolysis component further includes a temperature monitoring component 12 fixedly connected inside the chamber body 3.

[0027] In this embodiment, by setting the temperature monitoring component 12, when the temperature in the chamber body 3 displayed is convenient to reach the required temperature, the heating is stopped.

[0028] In a further preferred embodiment of the present utility model, as Figures 1-4 shown, a heating component 11 is provided on the outer wall of the chamber body 3, and the heating component 11 can be a heating sheet.

[0029] In this embodiment, when carrying out the ammonolysis reaction, the heating component 11 can provide the heat required for the ammonolysis reaction for the chamber body 3, and using the heating component 11 to heat the chamber body 3 can increase the heating area and shorten the heating time required.

[0030] In a further preferred embodiment of the present utility model, as Figures 2-4As shown, the fixing mechanism 7 includes mounting blocks 701 fixedly arranged at the front and rear ends on the left and right sides of the connecting plate 605. A connecting rod 702 that cooperates with the fixing groove 604 is slidably connected inside the mounting block 701. One end of the connecting rod 702 is fixedly connected to a support block 704, and a spring 703 is arranged outside the connecting rod 702.

[0031] In this embodiment, when the support block 704 moves, the spring 703 is stretched, so that the connecting rod 702 is pulled out of the fixing groove 604, and then the chamber body 3 is taken out from the mounting assembly 6.

[0032] In a further preferred embodiment of the present utility model, as Figures 2-4 shown, one end of the spring 703 is fixedly connected to one side of the mounting block 701, the other end of the spring 703 is fixedly connected to one side of the support block 704, and a handle 705 is fixedly connected to one side of the support block 704.

[0033] In this embodiment, through the provided handle 705, the subsequent installation and disassembly are facilitated.

[0034] It should be noted that for the foregoing embodiments, for the sake of simple description, they are all expressed as a series of action combinations. However, those skilled in the art should know that the present utility model is not limited by the described action sequence, because according to the present utility model, certain steps may be adopted in other sequences or simultaneously. Secondly, those skilled in the art should also know that the embodiments described in the specification are all preferred embodiments, and the actions and modules involved are not necessarily essential to the present utility model.

[0035] In several embodiments provided by the present application, it should be understood that the disclosed device can be implemented in other ways. For example, the device embodiments described above are only illustrative. For example, the above division of units can have other division methods in actual implementation. For example, multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed. Another point, the displayed or discussed coupling or communication connection between each other can be through some interfaces, and the indirect coupling or communication connection between devices or units can be in the form of telecommunications or other forms.

[0036] The units described above as separate components may or may not be physically separated. The components shown as units may or may not be physical units, that is, they can be located in one place, or they can be distributed to multiple network units. Some or all of the units can be selected according to actual needs to achieve the purpose of the solution of this embodiment.

[0037] The above embodiments are only used to illustrate the technical solutions of the present utility model, rather than to limit the protection scope of the utility model. Obviously, the described embodiments are only partial embodiments of the present utility model, rather than all embodiments. Based on these embodiments, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present utility model. Although the present utility model has been described in detail with reference to the above embodiments, those of ordinary skill in the art can still, without conflict and without creative efforts, combine, add or delete or make other adjustments to the features in the various embodiments of the present utility model according to the circumstances, so as to obtain different technical solutions that essentially do not deviate from the concept of the present utility model, and these technical solutions also belong to the scope of protection of the present utility model.

Claims

1. A DNA synthesizer with an ammonolysis component, characterized in that, It includes a DNA synthesizer body (1). A cavity (2) is opened at the bottom of the inner cavity of the DNA synthesizer body (1). Two groups of movably arranged front covers are installed at the front end of the cavity (2). A moving component is provided in the cavity (2), and a connecting piece (10) is arranged on the moving component. An installation plate (5) is fixedly connected to the connecting piece (10). An installation component (6) is fixedly arranged on the top of the installation plate (5). A chamber body (3) is arranged on the installation component (6). Two groups of fixing mechanisms (7) are symmetrically arranged on both sides of the installation component (6) to facilitate the limit fixation of the chamber body (3). A synthesis component, a waste discharge component, and an ammonolysis component are installed on the upper surface of the DNA synthesizer body (1), and the synthesis component, the waste discharge component, and the ammonolysis component are communicated with the chamber body (3). The installation component (6) includes sliders (603) fixedly connected to both sides of the chamber body (3), and a connecting plate (605) fixedly arranged on the installation plate (5). An installation groove (602) is opened in the connecting plate (605). Chute grooves (601) are opened at the front and rear ends inside the installation groove (602). The chamber body (3) is slidably connected in the chute grooves (601) through the sliders (603) on both sides. Fixing grooves (604) are opened at the front and rear ends on the left and right sides of the sliders (603).

2. The DNA synthesizer with an ammonolysis component according to claim 1, wherein The ammonolysis component includes a seal (13). An air inlet (8) and an exhaust port (9) are arranged on the seal (13).

3. The DNA synthesizer with an ammonolysis component according to claim 2, characterized in that, The ammonolysis component further includes a temperature monitoring component (12) fixedly connected inside the chamber body (3).

4. The DNA synthesizer with an ammonolysis component according to claim 3, characterized in that, A heating component (11) is arranged on the outer wall of the chamber body (3), and the heating component (11) can be a heating sheet.

5. The DNA synthesizer with an ammonolysis component according to claim 3, characterized in that The fixing mechanism (7) includes mounting blocks (701) fixedly arranged at the front and rear ends on the left and right sides of the connecting plate (605). A connecting rod (702) that cooperates with the fixing groove (604) is slidably connected inside the mounting block (701). One end of the connecting rod (702) is fixedly connected to a support block (704). A spring (703) is arranged on the outer side of the connecting rod (702).

6. The DNA synthesizer with an ammonolysis component according to claim 5, characterized in that, One end of the spring (703) is fixedly connected to one side of the mounting block (701), and the other end of the spring (703) is fixedly connected to one side of the support block (704). A handle (705) is fixedly connected to one side of the support block (704).

7. The DNA synthesizer with an ammonolysis component according to claim 1, characterized in that, The mounting block (701) is arranged in an L shape. The fixing groove (604) is adapted to the connecting rod (702), and the fixing groove (604) is movably connected to the connecting rod (702).

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