Inoculation device for drug sensitive test

Abstract

The utility model discloses an inoculation device for a drug sensitive test. According to the inoculation device, through holes are formed in a culture plate; the open ends of the culture tubes are fixed with the culture plate, and the through holes are limited in the culture tubes; inoculating needles are fixed on the lower plate surface of the inoculating plate, and the inoculating ends of the inoculating needles can be inserted into the culture tubes; a limiting groove is formed in the rack, a receding hole is formed in the groove bottom wall of the limiting groove, the culture plate and the culture dish can be embedded in the limiting groove, and the culture tube can be limited on the inner side of the receding hole; the guide rail is vertically arranged, the sliding block is vertically connected with the guide rail in a sliding mode, the movable frame is fixed to the sliding block, an inoculation plate is detachably fixed to the movable frame, and the inoculation needle can be located over the through hole. The multiple inoculation needles can be inserted into the culture dish or the multiple culture tubes at the same time, and therefore synchronous inoculation of a large number of strains is achieved; in the inoculation process, the culture plate and the inoculation plate can be guaranteed to be horizontally arranged and cannot move horizontally at will, the inoculation needle is right opposite to the vertical position of the through hole, and cross contamination cannot occur in the inoculation process.

Description

Technical Field

[0001] The utility model relates to the field of biological experimental equipment, and more specifically to an inoculation device for drug sensitivity test. Background Art

[0002] Different pathogenic bacteria have different sensitivities to different antibacterial drugs, and different strains of the same bacterium also have differences in sensitivities to different antibacterial drugs. Therefore, measuring the sensitivity of pathogenic bacteria to antibacterial drugs under in vitro conditions is an important method for guiding clinical medication, and it has important significance in improving the effect of antibacterial drugs, reducing drug use, and alleviating the phenomenon of multi-drug resistant strains. At present, the methods for bacteriostatic tests mainly include qualitative determination methods such as disk diffusion method, Oxford cup method, punching method, etc., and semi-quantitative determination methods such as dilution method, E-test, etc. Among them, the dilution method for drug sensitivity test can be further divided into liquid dilution method and agar dilution method due to the different media used.

[0003] The agar dilution method, also known as the plate dilution method, is to dilute a certain concentration of antibacterial drug and mix it with agar to make a culture medium, then inoculate the test strain, and observe the growth of bacteria after incubation to obtain the sensitivities of different pathogenic bacteria to different concentrations of antibacterial drugs. This method can simultaneously conduct drug sensitivity tests on a large number of strains, significantly saving the test cost.

[0004] In the operation process of the existing agar dilution method, the method of spotting multiple strains one by one is generally adopted, lacking equipment that can realize synchronous inoculation of a large number of strains, resulting in problems such as long operation time, large consumption of pipette tips, and easy confusion. The multi-point inoculators self-made by some laboratories are mostly used in combination with 96-well deep well plates. Since the distance between the wells of the deep well plate is very small, during the process of the multi-point inoculator entering and exiting the deep well plate, due to manual inoculation operation, the inoculation needle is prone to contact with the adjacent well walls, resulting in cross-contamination between samples and affecting the accuracy of test results.

[0005] Therefore, how to provide an inoculation device for drug sensitivity test to overcome the above problems is an urgent problem to be solved by those skilled in the art. Summary of the Utility Model

[0006] In view of this, the utility model provides an inoculation device for drug sensitivity test.

[0007] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0008] An inoculation device for drug sensitivity test, comprising:

[0009] A culture plate, which is horizontally arranged and has a plurality of through holes on its surface;

[0010] A culture tube, wherein the culture tube is provided in plurality and the lower ends thereof are closed, the upper ends of the plurality of culture tubes are sealed and fixed to the culture plate, and the plurality of through holes are respectively limited inside the opening ends of the plurality of culture tubes;

[0011] An inoculation plate, wherein the inoculation plate is arranged horizontally and a plurality of inoculation needles are vertically fixed on the lower plate surface thereof, wherein the plurality of inoculation needles can be directly opposite to the respective positions of the plurality of through holes, and the inoculation ends of the plurality of inoculation needles can be simultaneously inserted into the plurality of culture tubes;

[0012] A rack, wherein a limiting groove is provided on the rack, and a clearance hole is provided on the bottom wall of the limiting groove, so that the lower plate surface of the culture plate can be closely attached to the bottom wall of the limiting groove, and the plurality of culture tubes can be limited inside the clearance hole;

[0013] A culture dish, wherein the culture dish can be placed horizontally in the limiting groove, and the inoculation ends of the plurality of inoculation needles can be inserted into the plurality of culture dishes at the same time;

[0014] The telescopic part includes a guide rail, a slider and a movable frame, the guide rail is arranged vertically, the slider is vertically slidably connected to the guide rail, the movable frame is fixed to the slider, the inoculation plate is detachably fixed on the movable frame, and the plurality of inoculation needles can be located directly above the plurality of through holes.

[0015] It can be known from the above technical scheme that compared with the prior art, the utility model discloses an inoculation device for drug sensitivity test. The culture plate in the present application is provided with multiple culture tubes, and multiple inoculation needles are fixed on the inoculation plate. Multiple inoculation needles can be inserted into culture dishes or multiple culture tubes at the same time, so as to realize the synchronous inoculation of a large number of strains; the culture plate and the culture dish can be tightly embedded in the limiting groove. During the inoculation process, the culture plate and the culture dish can ensure horizontal arrangement, and the culture plate and the culture dish will not move horizontally in the limiting groove at will; by designing the guide rail, the slider and the mobile frame, the mobile frame connected to the slider can move along the length direction of the guide rail, and the inoculation plate is detachably connected to the mobile frame. When the inoculation plate moves vertically, the inoculation plate can maintain a horizontal arrangement, and at the same time, multiple inoculation needles are vertically opposite to each other at multiple through holes, ensuring that during the inoculation process, multiple inoculation needles are in contact with the inner wall of the through hole or the culture tube to prevent cross contamination.

[0016] Preferably, the culture plate and the inoculation plate are both rectangular plates, a plurality of through holes are evenly opened on the culture plate, a plurality of inoculation needles are evenly fixed on the inoculation plate, and a handle is fixed in the center of the upper plate surface of the inoculation plate. A large number of strains can be inoculated at a time, and the operator can easily hold and transfer the inoculation plate.

[0017] Preferably, the limiting groove is a rectangular groove with one end open, and the two opposite outer side walls and one end wall of the culture plate can be arranged closely with one end wall and two groove side walls of the limiting groove respectively. The culture plate will not move horizontally in the limiting groove at will.

[0018] Preferably, two guide rails and two sliders are provided, each guide rail is vertically slidably connected to one slider, and the moving frame is fixed to the two sliders at the same time. The moving frame can stably move vertically.

[0019] Preferably, the movable frame is provided with a placement slot with an open end, the bottom wall of the placement slot is provided with a clearance notch, the inoculation plate can be tightly embedded in the placement slot, and the plurality of inoculation needles are located inside the clearance notch; a baffle bar is integrally formed horizontally on each of the two opposite inner side walls of the placement slot, and the upper plate surface of the inoculation plate can abut against the two baffle bars at the same time. The inoculation plate can be reliably limited in the placement slot.

[0020] Preferably, a scale line is vertically arranged on the side wall of one of the guide rails, and a triangular indicating protrusion is arranged on the movable frame, and the sharp end of the indicating protrusion can be aligned with the scale line. The downward movement distance of the inoculation plate can be clearly known and recorded.

[0021] Preferably, a positioning pin is further included, a first insertion hole is provided on one of the guide rails, the first insertion hole is located above the frame, a second insertion hole is provided on the mobile frame, and one end of the positioning pin can be inserted into both the first insertion hole and the second insertion hole at the same time. The relative position of the mobile frame and the guide rail can remain unchanged, which is convenient for disassembly and assembly of the inoculation plate. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings in the following description are only embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0023] Figure 1 It is an overall axonometric diagram of an inoculation device for drug sensitivity testing equipped with a culture plate;

[0024] Figure 2 It is an overall axonometric view of an inoculation device for drug sensitivity testing equipped with a culture dish;

[0025] Figure 3 It is a partial axonometric view of an inoculation device for drug sensitivity testing;

[0026] Figure 4 yes Figure 3 A partial enlarged view of point A in the middle.

[0027] In the figure:

[0028] 01 is a culture plate, 010 is a through hole, 02 is a culture tube, 03 is an inoculation plate, 04 is an inoculation needle, 05 is a frame, 050 is a limiting groove, 051 is a relief hole, 06 is a guide rail, 060 is a scale line, 061 is a first jack, 07 is a slider, 08 is a moving frame, 080 is a placement groove, 081 is a relief notch, 082 is a retaining bar, 083 is an indicating protrusion, 084 is a second jack, 09 is a handle, 10 is a positioning pin, and 11 is a petri dish. Specific embodiments

[0029] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0030] The present invention discloses an inoculation device for drug sensitivity test. In the culture plate 01 of the present application, a plurality of culture tubes 02 are provided. A plurality of inoculation needles 04 are fixed on the inoculation plate 03. The plurality of inoculation needles 04 can be simultaneously inserted into the petri dish 11 or a plurality of culture tubes 02, so as to realize the synchronous inoculation of a large number of strains.

[0031] By designing the petri dish 11, the antibacterial drug mixed with agar can be contained in the petri dish 11. The plurality of inoculation needles 04 can synchronously enter the petri dish 11 and uniformly contact the mixture in the petri dish 11.

[0032] By designing the handle 09, this design facilitates the operator to transfer and operate the inoculation plate 03.

[0033] By designing the frame 05, a limiting groove 050 is provided on the frame 05. The culture plate 01 and the petri dish 11 can be tightly fitted in the limiting groove 050. During the inoculation process, the culture plate 01 and the petri dish 11 can be guaranteed to be horizontally arranged, and the culture plate 01 and the petri dish 11 will not move horizontally randomly in the limiting groove 050.

[0034] By designing the guide rail 06, the slider 07 and the moving frame 08, the moving frame 08 connected to the slider 07 can move along the length direction of the guide rail 06. The inoculation plate 03 is detachably connected to the moving frame 08. While the inoculation plate 03 moves vertically, the inoculation plate 03 can maintain a horizontal arrangement. At the same time, the plurality of inoculation needles 04 are respectively vertically aligned with the plurality of through holes 010, ensuring that during the inoculation process, the plurality of inoculation needles 04 contact the inner side walls of the through holes 010 or the culture tubes 02, preventing cross-contamination.

[0035] By designing the scale line 060 and the indicating protrusion 083, the downward movement distance of the moving frame 08 can be known and recorded, and the depth of the inoculation needle 04 inserted into the culture tube 02 or the petri dish 11 can be controlled;

[0036] By designing the positioning pin 10, the first jack 061 and the second jack 084, when the inoculation device is not in use, the moving frame 08 can be positioned by the positioning pin 10, and the moving frame 08 will not move vertically along the guide rail 06;

[0037] By designing the placement groove 080 and the retaining strip 082, the inoculation plate 03 can be stably arranged horizontally, and the inoculation plate 03 will not move horizontally or vertically randomly.

[0038] Embodiment

[0039] See the appendix Figures 1-4 It is a schematic diagram of the overall and partial structures of an embodiment of the present utility model. The present utility model specifically discloses an inoculation device for drug sensitivity test, including:

[0040] A culture plate 01, a rectangular culture plate 01 is horizontally arranged, and a plurality of circular through holes 010 are provided on the plate surface. In this embodiment, the culture plate 01 is a square plate with a side length of 100 mm, and 64 through holes 010 are provided on the culture plate 01. The 64 through holes 010 adopt an 8-row and 8-column layout;

[0041] Culture tubes 02, there are a plurality of culture tubes 02 and their lower ends are closed. The upper ends of the plurality of culture tubes 02 are hermetically fixed to the culture plate 01, and the plurality of through holes 010 are respectively limited inside the opening ends of the plurality of culture tubes 02. That is, there are also 64 culture tubes 02 in this embodiment, and the inside of the culture tubes 02 can contain the bacterial liquid of the strains to be tested;

[0042] An inoculation plate 03, a rectangular inoculation plate 03 is horizontally arranged and a plurality of inoculation needles 04 are vertically fixed on its lower plate surface. The inoculation needles 04 are of the prior art. The plurality of inoculation needles 04 can be respectively aligned with the positions of the plurality of through holes 010. The aperture of the through hole 010 is larger than the outer diameter of the inoculation needle 04. In this embodiment, there are also 64 inoculation needles 04, and the 64 inoculation needles 04 also adopt an 8-row and 8-column layout. By moving the inoculation plate 03 downward, the inoculation ends of the plurality of inoculation needles 04 can be simultaneously inserted into the plurality of culture tubes 02;

[0043] A frame 05, the frame 05 is stably placed on a horizontal plane. A limiting groove 050 is provided on the frame 05. The bottom wall of the limiting groove 050 is horizontally arranged, and a rectangular relief hole 051 is provided in the center of the bottom wall of the limiting groove 050. The lower plate surface of the culture plate 01 can be in close contact with the bottom wall of the limiting groove 050, and the plurality of culture tubes 02 can be limited inside the relief hole 051; the frame 05 can support and limit the culture plate 01;

[0044] Petri dish 11 can be horizontally placed in the limiting groove 050, and the antibacterial drug mixed with agar can be contained in the Petri dish 11. The outer contour shape and size of the Petri dish 11 in this application are the same as those of the culture plate 01, that is, the Petri dish 11 in this embodiment is a square dish with a side length of 100 mm.

[0045] The telescopic member includes a guide rail 06, a slider 07 and a moving frame 08. The guide rail 06 is arranged vertically. The slider 07 is vertically slidably connected to the guide rail 06. The slider 07 can reciprocally slide along the length direction of the guide rail 06. The moving frame 08 is fixed to the slider 07, and an inoculation plate 03 is detachably fixed on the moving frame 08. Multiple inoculation needles 04 can be located directly above multiple through holes 010. The user can move the moving frame 08 up and down to realize the synchronous vertical movement of multiple inoculation needles 04.

[0046] A handle 09 is fixedly centered on the upper plate surface of the inoculation plate 03. The user can hold the handle 09 to transfer the inoculation plate 03.

[0047] The limiting groove 050 is a rectangular groove with one end open. Two opposite outer side walls and one end wall of the culture plate 01 can be respectively in close contact with one end wall and two groove side walls of the limiting groove 050. Two opposite outer side walls and one end wall of the Petri dish 11 can be respectively in close contact with one end wall and two groove side walls of the limiting groove 050. After the culture plate 01 or the Petri dish 11 is arranged in the limiting groove 050, the culture plate 01 or the Petri dish 11 will not move randomly, ensuring that during the process of the inoculation needle 04 moving down into the culture tube 02, the inoculation needle 04 will not contact the inner side wall of the culture plate 01 or the culture tube 02.

[0048] In this application, in order to improve the stability of the moving frame 08 when sliding up and down, there are two guide rails 06 and two sliders 07. One slider 07 is vertically slidably connected to each guide rail 06, and the moving frame 08 is fixed to both sliders 07 at the same time.

[0049] The moving frame 08 is provided with a placement groove 080 with one end open. The bottom wall of the placement groove 080 is provided with a rectangular relief notch 081. The horizontally arranged inoculation plate 03 can be inserted into the placement groove 080 from the open end of the placement groove 080. At the same time, the inoculation needles 04 can also enter the relief notch 081. The inoculation plate 03 can be tightly fitted in the placement groove 080, and the inoculation plate 03 will not move horizontally randomly. Multiple inoculation needles 04 are all located inside the relief notch 081. One retaining strip 082 is horizontally integrally formed on each of the two opposite inner side walls of the placement groove 080. The upper plate surface of the inoculation plate 03 can be in contact with both retaining strips 082 at the same time, and the inoculation plate 03 in the placement groove 080 will not move up and down randomly.

[0050] On the side wall of a guide rail 06, scale lines 060 are provided vertically. On the moving frame 08, a triangular indicating protrusion 083 is provided, and the sharp end of the indicating protrusion 083 can be aligned with the scale lines 060. By designing the scale lines 060 and the indicating protrusion 083, the distance that the inoculation plate 03 moves downward can be accurately identified and recorded.

[0051] More specifically, it further includes a positioning pin 10. On a guide rail 06, a circular socket one 061 is provided, and the socket one 061 is located above the machine frame 05. On the moving frame 08, a circular socket two 084 is provided, and one end of the positioning pin 10 can be inserted into the socket one 061 and the socket two 084 at the same time. When the inoculation device is not in use or before the inoculation plate 03 is inserted into the placement groove 080, the positioning pin 10 can be used to position the moving frame 08, and the moving frame 08 will not move vertically along the guide rail 06.

[0052] When the inoculation device is in use:

[0053] In the initial state, the positioning pin 10 is inserted into the socket one 061 and the socket two 084 at the same time;

[0054] First step, the operator dilutes different concentrations of antibacterial drugs in a two-fold ratio and mixes them with agar, and then loads them into multiple culture dishes 11 respectively;

[0055] Second step, the operator equally adds the bacterial liquid cultured from the test strain into multiple culture tubes 02;

[0056] Third step, the operator places the culture plate 01 in the second step into the limit groove 050;

[0057] Fourth step, the operator places the inoculation plate 03 into the placement groove 080;

[0058] Fifth step, the operator holds the moving frame 08 with one hand and removes the positioning pin 10 with the other hand. After the positioning pin 10 is removed, slowly lower the moving frame 08, and multiple inoculation needles 04 synchronously enter multiple culture tubes 02. Multiple inoculation needles 04 contact the test strain synchronously and evenly, and the test strain evenly adheres to the lower ends of the inoculation needles 04;

[0059] Sixth step, after the inoculation needles 04 contact the test strain, push the moving frame 08 upward and install the positioning pin 10 in place;

[0060] Step 7: Withdraw the culture plate 01 from the limit slot 050, place one of the culture dishes 11 in Step 1 into the limit slot 050. Then, an operator holds the moving frame 08 with one hand and removes the positioning pin 10 with the other hand. After the positioning pin 10 is removed, slowly lower the moving frame 08, and multiple inoculation needles 04 simultaneously come into contact with the mixture of the antibacterial agent and agar in the culture dish. The test strains attached to the inoculation needles 04 will be inoculated into this mixture;

[0061] Step 8: After Step 7 is completed, push up the moving frame 08 again and replace the culture dish 11 until multiple culture dishes 11 in Step 1 are all inoculated with the test strains; transfer the inoculated culture dishes 11 to an incubator for cultivation, and observe the results after a certain period of time.

[0062] In this specification, each embodiment is described in a progressive manner. The key point of each embodiment is to illustrate the differences from other embodiments. For the same or similar parts among the embodiments, reference can be made to each other.

[0063] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be obvious to those skilled in the art. The general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. An inoculation device for drug sensitivity test, characterized in that, Comprising: A culture plate (01), on the horizontal surface of which there are a plurality of through holes (010); A plurality of culture tubes (02), the lower ends of which are closed, and the upper ends of the plurality of culture tubes (02) are hermetically fixed to the culture plate (01), and the plurality of through holes (010) are respectively limited inside the open ends of the plurality of culture tubes (02); An inoculation plate (03), which is horizontally arranged and has a plurality of inoculation needles (04) vertically fixed to its lower plate surface. The plurality of inoculation needles (04) can be respectively aligned with the positions of the plurality of through holes (010), and the inoculation ends of the plurality of inoculation needles (04) can be simultaneously inserted into the plurality of culture tubes (02); A frame (05), on which there is a limiting groove (050), and a relief hole (051) is provided on the bottom wall of the limiting groove (050). The lower plate surface of the culture plate (01) can be in close contact with the bottom wall of the limiting groove (050), and the plurality of culture tubes (02) can be limited inside the relief hole (051); A culture dish (11), which can be horizontally placed in the limiting groove (050), and the inoculation ends of the plurality of inoculation needles (04) can be simultaneously inserted into the plurality of culture dishes (11); A telescopic member, which includes a guide rail (06), a slider (07) and a moving frame (08). The guide rail (06) is vertically arranged, the slider (07) is vertically slidably connected to the guide rail (06), the moving frame (08) is fixed to the slider (07), and the inoculation plate (03) is detachably fixed to the moving frame (08), and the plurality of inoculation needles (04) can be located directly above the plurality of through holes (010).

2. The inoculation device for drug sensitivity test according to claim 1, characterized in that, Both the culture plate (01) and the inoculation plate (03) are rectangular plates. A plurality of the through holes (010) are uniformly formed in the culture plate (01), and a plurality of the inoculation needles (04) are uniformly fixed to the inoculation plate (03); A handle (09) is centrally fixed to the upper plate surface of the inoculation plate (03).

3. The inoculation device for drug sensitivity test according to claim 2, characterized in that The limiting groove (050) is a rectangular groove with one end open, and two opposite outer side walls and one end wall of the culture plate (01) can be respectively in close contact with one end wall and two side walls of the limiting groove (050).

4. The inoculation device for drug sensitivity test according to claim 1, characterized in that, There are two guide rails (06) and two sliders (07). Each guide rail (06) is vertically slidably connected with a slider (07), and the moving frame (08) is simultaneously fixed to the two sliders (07).

5. The inoculation device for drug sensitivity test according to claim 4, characterized in that, The moving frame (08) is provided with a placement groove (080) with one end open, and a relief notch (081) is provided on the bottom wall of the placement groove (080). The inoculation plate (03) can be tightly fitted into the placement groove (080), and the plurality of inoculation needles (04) are all located inside the relief notch (081); One retaining strip (082) is horizontally integrally formed on each of the two opposite inner side walls of the placement groove (080), and the upper plate surface of the inoculation plate (03) can be simultaneously abutted against the two retaining strips (082).

6. The inoculation device for drug sensitivity test according to claim 4, characterized in that, On the side wall of one of the guide rails (06), scale lines (060) are provided vertically. A triangular indicating protrusion (083) is provided on the moving frame (08), and the sharp end of the indicating protrusion (083) can be aligned with the scale lines (060).

7. The inoculation device for drug sensitivity test according to claim 4, characterized in that, It further includes a positioning pin (10). A first jack (061) is provided on one of the guide rails (06). The first jack (061) is located above the frame (05). A second jack (084) is provided on the moving frame (08). One end of the positioning pin (10) can be inserted into both the first jack (061) and the second jack (084) simultaneously.

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