Convenient cell lysis solution auxiliary collection device
By designing a convenient cell lysate-assisted collection device, the combined structure of the support plate and the adjustment plate is used to solve the problem of time-consuming and labor-intensive and unstable manual operation by the experimenter, and the stable placement of the six-well plate and efficient lysate collection are achieved.
Patent Information
- Application Number
- CN202422198735.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-09
- Publication Date
- 2025-07-18
- Estimated Expiration
- 2034-09-09
AI Technical Summary
In the prior art, experimenters operate the six-well plate with both hands to collect the cell lysate by tilting the cell lysate by tilting it, which easily leads to incomplete collection of lysate or spilling out.
A convenient cell lysate-assisted collection device is designed, including a support plate and an adjustment plate. It is connected by hinges. The tilt angle of the support plate is adjusted by adjusting the positioning plate and anti-detachment part. It ensures the stable placement of the six-well plate, and adjusts the angle through the rotating sleeve to achieve stepless adjustment.
It reduces manpower operation, improves the placement stability of the six-well plate and the collection efficiency of the lysate, prevents spilling, and improves the collection efficiency.
Smart Images
Figure CN223118463U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell experiment, in particular to a convenient cell lysate auxiliary collection device. Background Art
[0002] Western and IP lysis buffer is a lysis buffer used to lyse cell or tissue samples under non-denaturing conditions, which can prepare protein samples and is applicable to PAGE, Western, immunoprecipitation (IP), co-immunoprecipitation (co-IP) and ELISA, etc. RIPA lysis buffer is a traditional rapid cell and tissue lysis buffer, and the protein samples obtained by lysing with RIPA lysis buffer can be used for conventional Western, IP, etc. During cultivation, Western and IP lysis buffer or RIPA lysis buffer is added into a six-well plate, and then incubated on ice for 30 min. After that, the cell lysate is aspirated and transferred into a 1.5 mL EP tube.
[0003] In the later stage of the cultivation process, after the cells are lysed, since the volume of the cell lysate in the six-well plate is small, we usually tilt the six-well plate at a certain angle by hand. After the lysate accumulates, the other hand operates the pipette to aspirate. However, this process requires the experimenter to operate with both hands, which is time-consuming and laborious; moreover, when operating with both hands at the same time, the tilting operation of one hand on the six-well plate is not very stable. The six-well plate may not be tilted enough to cause incomplete collection of the lysate, or may be tilted too much to cause the lysate to spill out. Summary of the Utility Model
[0004] In order to solve the technical problems in the prior art that it is time-consuming and laborious for the experimenter to operate with both hands, and when operating with both hands at the same time, the tilting operation of the six-well plate is not very stable, the six-well plate may not be tilted enough to cause incomplete collection of the lysate, or may be tilted too much to cause the lysate to spill out, the utility model provides the following technical solutions.
[0005] A convenient cell lysate auxiliary collection device of the utility model includes a support plate and an adjustment plate which are oppositely arranged and connected by a plurality of hinge pieces at the upper end. The lower end of the support plate extends obliquely upward with a positioning plate for positioning the six-well plate. The upper end of the positioning plate bends and extends towards the support plate with an anti-detachment part. On the side where the support plate and the adjustment plate are close to each other, an adjustment component is connected. The adjustment component includes a first hinge piece and a second hinge piece which are connected to the support plate and the adjustment plate. The first hinge piece and the second hinge piece are respectively hinged with a first screw column and a second screw column with opposite thread directions which are oppositely arranged. The first screw column and the second screw column are connected with a rotating sleeve. The rotation of the rotating sleeve makes the first screw column and the second screw column approach or move away from each other, so as to adjust the tilting angle of the support plate.
[0006] As a further technical solution, the rotating sleeve includes an internal thread portion threadedly connected to the first stud and the second stud respectively, and a limiting cavity with an inner diameter larger than that of the internal thread portion. The adjacent ends of the first stud and the second stud are provided with a first limiting end and a second limiting end that match the limiting cavity.
[0007] As a further technical solution, the positioning plate is provided with a plurality of through grooves for facilitating the taking of the six-well plate.
[0008] As a further technical solution, a plurality of uniformly arranged anti-slip pads are provided on the surface of the support plate for placing the six-well plate.
[0009] As a further technical solution, a sliding member is further connected to the adjacent sides of the support plate and the adjusting plate, and the sliding member moves as the adjusting member moves.
[0010] As a further technical solution, the support plate and the positioning plate are perpendicularly arranged.
[0011] The beneficial effects of the present utility model: The cell lysate auxiliary collection device of the present utility model can place the six-well plate between the support plate and the positioning plate, eliminating the need for experimenters to hold the six-well plate by hand and reducing manual operation; moreover, anti-slip pads are provided on the surface of the support plate, improving the placement stability of the six-well plate, and the inclination angle of the support plate is steplessly adjusted by rotating the adjusting member, enabling the six-well plate to be at the correct inclination angle, facilitating the collection of the lysate in the six-well plate, preventing the lysate from spilling out, and improving the collection efficiency of the lysate at the same time. BRIEF DESCRIPTION OF THE DRAWINGS
[0012] Figure 1 is a schematic structural view of the convenient cell lysate auxiliary collection device of the present utility model;
[0013] Figure 2 is a schematic structural view of another perspective of the convenient cell lysate auxiliary collection device of the present utility model;
[0014] Figure 3 is an exploded sectional view of the adjusting member of the convenient cell lysate auxiliary collection device of the present utility model;
[0015] In the figure: 1 - support plate; 2 - adjusting plate; 3 - hinge member; 4 - positioning plate; 401 - anti - detachment portion; 402 - through groove; 5 - adjusting member; 501 - first hinge member; 502 - second hinge member; 503 - first stud; 504 - second stud; 505 - rotating sleeve; 506 - internal thread portion; 507 - limiting cavity; 508 - first limiting end; 509 - second limiting end; 6 - sliding member; 7 - anti - slip pad. DETAILED DESCRIPTION OF THE EMBODIMENTS
[0016] In order to make the objectives, technical solutions and advantages of the present utility model more clearly understood, the following further describes the present utility model in detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present utility model and are not used to limit the present utility model. It should be noted that, without conflict, the embodiments in the present utility model and the features in the embodiments may be combined with each other.
[0017] In the description of the present utility model, it should be understood that the terms "upper" and "lower" are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present utility model. In addition, the terms "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the quantity of the indicated technical features. In the description of the present utility model, unless otherwise specified, the meaning of "a plurality of" is two or more.
[0018] As Figure 1 and Figure 2 shown, in a preferred embodiment, a convenient cell lysate auxiliary collection device of the present utility model is used to place a six-well plate obliquely, without the need for an experimenter to hold the six-well plate by hand, reducing manual operation. It includes a support plate 1 and an adjustment plate 2 arranged oppositely. The upper ends of the support plate 1 and the adjustment plate 2 are connected by a plurality of hinge members 3. The support plate 1 and the adjustment plate 2 are acrylic plates, and the upper ends of the two can be respectively bonded with hinge members 3 made of acrylic material. Thus, the hinge members 3 at the upper ends of the support plate 1 and the adjustment plate 2 can make the two rotate relative to each other, changing the inclination of the surface of the support plate 1 for placing the six-well plate.
[0019] The lower end of the support plate 1 extends obliquely upward with a positioning plate 4 for positioning the six-well plate. The support plate 1 and the positioning plate 4 are perpendicularly arranged. When the six-well plate is placed on the upper surface of the support plate 1, the lower end of the six-well plate abuts against the positioning plate 4. The upper end of the positioning plate 4 bends and extends towards the support plate 1 with an anti-disengagement portion 401. The anti-disengagement portion 401 and the positioning plate 4 are integrally formed, which can prevent the six-well plate from moving horizontally or falling off.
[0020] In a preferred embodiment, an adjusting member 5 is connected to the adjacent sides of the support plate 1 and the adjusting plate 2, and a sliding member 6 is also connected to the adjacent sides of the support plate 1 and the adjusting plate 2. The sliding member 6 can move as the adjusting member 5 moves. Specifically, the adjusting member 5 includes a first hinge member 501 and a second hinge member 502 connected to the support plate 1 and the adjusting plate 2. The first hinge member 501 and the second hinge member 502 are fixedly connected to the support plate 1 and the adjusting plate 2 respectively. The first hinge member 501 and the second hinge member 502 are respectively hinged with a first screw rod 503 and a second screw rod 504 having opposite thread directions arranged oppositely. The first screw rod 503 and the second screw rod 504 are connected with a rotating sleeve 505. By screwing the rotating sleeve 505, the rotation of the rotating sleeve 505 can make the first screw rod 503 and the second screw rod 504 approach or move away from each other. Thus, the support plate 1 and the adjusting plate 2 can be relatively close or move away from each other, so as to adjust the inclination angle of the six-hole plate on the upper surface of the support plate 1, which is convenient for sucking and collecting the lysate in the six-hole plate.
[0021] The rotating sleeve 505 includes internal thread portions 506 respectively threadedly connected to the first screw rod 503 and the second screw rod 504. The two internal thread portions 506 are respectively threadedly connected to the first screw rod 503 and the second screw rod 504. Since the thread directions of the first screw rod 503 and the second screw rod 504 are opposite, when the rotating sleeve 505 is screwed, the first screw rod 503 and the second screw rod 504 approach or move away from each other. At the same time, a limiting cavity 507 with an inner diameter larger than that of the internal thread portion 506 is provided in the inner cavity of the rotating sleeve 505. The adjacent ends of the first screw rod 503 and the second screw rod 504 are provided with a first limiting end 508 and a second limiting end 509 that match the limiting cavity 507, which can prevent the rotating sleeve 505 from being unscrewed. During assembly, the rotating sleeve 505 can be formed by bonding two parts with the same structure. After the two parts are respectively assembled with the first screw rod 503 and the second screw rod 504, the first limiting end 508 and the second limiting end 509 are bonded, and then the rotating sleeve 505 is bonded and formed.
[0022] In a preferred embodiment, the positioning plate 4 is provided with a plurality of through grooves 402 for facilitating the taking of the six-hole plate, which is convenient for the placement and picking up of the six-hole plate. At the same time, a plurality of uniformly arranged anti-slip pads 7 are provided on the surface of the support plate 1 for placing the six-hole plate. The anti-slip pads 7 can be made of rubber material and are bonded to the upper surface of the support plate 1.
[0023] When the present utility model is in use, first screw the adjusting member 5 to make the support plate 1 at a gentle inclination angle. Place the six-hole plate on the upper surface of the support plate 1 and make its lower end abut against the positioning plate 4. Then, according to the liquid level of the lysate in the six-hole plate, screw the rotating sleeve 505 to perform stepless adjustment on the inclination angle of the support plate 1 and the six-hole plate, so that the inclination angle is convenient for sucking and collecting the lysate.
[0024] The preferred specific embodiments and examples of the present utility model have been described in detail above in conjunction with the accompanying drawings. However, the present utility model is not limited to the above embodiments and examples. Within the scope of knowledge possessed by those skilled in the art, various changes or equivalent substitutions can be made without departing from the concept of the present utility model. Therefore, the present utility model is not limited by the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of this application belong to the scope protected by the present utility model.
Claims
1. A convenient cell lysate-assisted collection device, characterized in that: It includes a support plate (1) and an adjustment plate (2) which are oppositely arranged and connected at the upper end by a plurality of hinge parts (3). A positioning plate (4) for positioning a six-hole plate extends obliquely upward from the lower end of the support plate (1). An anti-disengagement part (401) extends bent towards the support plate (1) from the upper end of the positioning plate (4). An adjustment component (5) is connected to the side of the support plate (1) and the adjustment plate (2) that are close to each other. The adjustment component (5) includes a first hinge part (501) and a second hinge part (502) connected to the support plate (1) and the adjustment plate (2). The first hinge part (501) and the second hinge part (502) are respectively hinged with a first screw column (503) and a second screw column (504) with opposite thread directions that are oppositely arranged. The first screw column (503) and the second screw column (504) are connected to a rotating sleeve (505). Rotating the rotating sleeve (505) makes the first screw column (503) and the second screw column (504) approach or move away from each other, thereby adjusting the inclination angle of the support plate (1).
2. The convenient cell lysate-assisted collection device according to claim 1, wherein: The rotating sleeve (505) includes an internal thread part (506) threadedly connected to the first screw column (503) and the second screw column (504) respectively, and a limiting cavity (507) with an inner diameter larger than that of the internal thread part (506). The ends of the first screw column (503) and the second screw column (504) that are close to each other are provided with a first limiting end (508) and a second limiting end (509) that match the limiting cavity (507).
3. The convenient cell lysate-assisted collection device according to claim 1, wherein: The positioning plate (4) is provided with a plurality of through grooves (402) for facilitating the taking of the six-hole plate.
4. The convenient cell lysate-assisted collection device according to claim 1, characterized in that: On the surface of the support plate (1) for placing the six-hole plate, a plurality of uniformly arranged anti-slip pads (7) are provided.
5. The convenient cell lysate-assisted collection device according to claim 1, wherein: A sliding component (6) is also connected to the side of the support plate (1) and the adjustment plate (2) that are close to each other. The sliding component (6) moves as the adjustment component (5) moves.
6. The convenient cell lysate-assisted collection device according to claim 1, wherein: The support plate (1) is perpendicularly arranged with the positioning plate (4).