Digital visual culture vessel for antibacterial drug in-vitro drug sensitivity experiment

Abstract

The utility model discloses a digital visual culture vessel for an antibacterial drug in-vitro drug sensitivity experiment, which belongs to the technical field of culture vessels, and can realize effective interpretation of the size of an inhibition zone by directly observing scales at scale line areas during subsequent measurement of the size of the inhibition zone. Subsequent measurement through a vernier caliper and the like can be avoided as much as possible, the subsequent overall measurement work efficiency is improved, and meanwhile the later overall test cost is reduced. The culture vessel for the digital visual antibacterial drug in-vitro drug sensitivity experiment comprises a culture vessel bottom cover and a top cover covering the top of the culture vessel bottom cover, and a containing cavity for containing a medium is formed in the culture vessel bottom cover; the bottom of the culture dish bottom cover is carved with scale marks used for marking measurement, the zero scale of the scale marks is located at the middle section of the scale marks, the scales on the scale marks extend from the middle section to the two sides, and positioning holes are formed in the positions, corresponding to the centers of the scale marks, in the containing cavity.

Description

Technical Field

[0001] The utility model belongs to the technical field of culture vessels, and in particular relates to a culture vessel for in vitro drug sensitivity experiments of digitally visualized antibacterial drugs. Background Art

[0002] Current status of in vitro susceptibility testing of antimicrobial drugs: There are many commonly used methods for susceptibility testing, including the disc diffusion method, dilution method, and E-test method. Taking the disc diffusion method as an example, this method is to stick a piece of paper containing a quantitative antimicrobial drug on an agar plate inoculated with the pathogen to be tested. The antimicrobial drug on the paper will diffuse into the surrounding agar, forming a gradually decreasing drug concentration gradient. Since pathogenic bacteria have different sensitivities to various antimicrobial drugs, inhibition rings of different diameters will appear around the drug paper to inhibit the growth of pathogens. According to the presence and size of the inhibition ring, it can be determined whether the test bacteria are resistant or sensitive to the antimicrobial drug. The sensitivity evaluation of antimicrobial drugs is mainly carried out by measuring the size of the inhibition ring of the antimicrobial drug on the MH culture medium, and then judging whether it is resistant or sensitive according to the CLSI standards.

[0003] Most of the existing culture vessels for drug sensitivity tests can only perform simple drug sensitivity tests. In subsequent measurements, additional measuring tools such as vernier calipers are required to measure the diameter of the inhibition zone. The overall operation is relatively cumbersome. How to organically combine the overall culture of the drug sensitivity test and the subsequent measurement has become an urgent problem to be solved. Therefore, a digital visualization culture vessel for in vitro drug sensitivity test of antimicrobial drugs is needed to assist in solving this problem. Utility Model Content

[0004] (1) Technical issues to be solved

[0005] In view of the deficiencies in the prior art, the purpose of the utility model is to provide a digital visualized culture vessel for in vitro drug sensitivity tests of antimicrobial drugs. When subsequently measuring the size of the inhibition zone, the digital visualized culture vessel for in vitro drug sensitivity tests of antimicrobial drugs can directly observe the scale at the position of the scale line area to effectively understand the size of the inhibition zone, and can avoid subsequent measurement by vernier calipers, etc. as much as possible, thereby improving the subsequent overall measurement work efficiency and reducing the subsequent overall test cost.

[0006] (2) Technical solution

[0007] In order to solve the above technical problems, the utility model provides a digital visualized culture vessel for in vitro drug sensitivity test of antimicrobial drugs, the digital visualized culture vessel for in vitro drug sensitivity test of antimicrobial drugs comprising a culture vessel bottom cover and a top cover covering the top of the culture vessel bottom cover, wherein a holding cavity for holding a medium is arranged inside the culture vessel bottom cover;

[0008] The bottom of the culture dish bottom cover is engraved with scale lines for marking measurements, the zero scale of the scale lines is located in the middle of the scale lines, the scales on the scale lines extend from the middle to both sides, and a positioning hole is opened in the accommodating cavity corresponding to the center of the scale lines.

[0009] As a further preferred embodiment, there are six scale lines, and the six scale lines are arranged in a circular array with the axis of the bottom cover of the culture dish as the center.

[0010] As a further preferred solution, the angle between the two scale lines is 60 degrees, and the positioning hole is located at the zero scale position of the scale lines.

[0011] As a further preferred solution, a protruding seat is protrudingly provided at the bottom of the bottom cover of the culture dish, and the bottom of the top cover is arranged on the top of the protruding seat.

[0012] As a further preferred embodiment, a plug-in slot is provided at the bottom of the culture dish bottom cover between two corresponding scale lines, a plug-in board is installed at the plug-in slot, and a receiving slot for placing the corresponding name is provided at the top of the plug-in board.

[0013] As a further preferred solution, limit strips are symmetrically protruding on both sides of the plug-in slot, and limit slots are symmetrically provided on both sides of the plug-in board, and the limit strips are plugged in with the limit slots.

[0014] As a further preferred solution, a non-slip pad is installed on the middle section of the outer edge of the culture dish bottom cover, the outer edge of the non-slip pad fits with the inner edge of the top cover, and the culture dish bottom cover, protruding seat and top cover are all made of transparent materials.

[0015] (3) Beneficial effects

[0016] Compared with the prior art, the beneficial effects of the utility model are:

[0017] The utility model can perform auxiliary scale processing on the bottom of the culture dish bottom cover in advance during operation by engraving the scale lines at the bottom of the culture dish bottom cover, and can directly observe the scale at the scale line area position when measuring the size of the inhibition zone in the subsequent process to effectively understand the size of the inhibition zone, and can avoid subsequent measurement by vernier calipers and the like as much as possible, thereby improving the subsequent overall measurement work efficiency and reducing the subsequent overall test cost;

[0018] Through the cooperation between the plug-in board inserted and installed at the plug-in slot and the accommodation groove at the top of the plug-in board, etc., the name of the corresponding drug can be placed at the accommodation groove before the drug sensitivity test, which is convenient for observation and subsequent recording. At the same time, it also tries to avoid directly writing the drug name at the bottom of the bottom cover of the culture dish, which requires subsequent secondary cleaning and other situations, improving the overall practicality of the culture vessel. Brief Description of the Drawings

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present application. For those skilled in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0020] Figure 1 is a schematic structural diagram of the present utility model;

[0021] Figure 2 is a bottom view of the present utility model;

[0022] Figure 3 is a longitudinal sectional view of the present utility model;

[0023] Figure 4 is the present utility model Figure 1 an enlarged structural schematic diagram of part A in.

[0024] The reference numerals in the drawings are: 1, bottom cover of the culture dish; 2, protruding seat; 3, plug-in slot; 31, limiting strip; 4, plug-in board; 41, limiting groove; 42, accommodation groove; 5, anti-slip pad; 6, scale line; 7, positioning hole; 8, top cover. Detailed Description of the Embodiment

[0025] The following will clearly and completely describe the technical solutions in the embodiments of the present utility model with reference to the drawings in the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, rather than all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0026] This specific embodiment is a culture vessel for digital visualization of in vitro drug sensitivity test of antibacterial drugs, such as Figure 1 and Figure 2As shown, the digital visualized culture vessel for in vitro drug sensitivity test of antimicrobial drugs includes a culture dish bottom cover 1 and a top cover 8 covering the top of the culture dish bottom cover 1, the culture dish bottom cover 1 is provided with a holding cavity for holding a medium, the culture dish bottom cover 1 is provided with a holding cavity for holding a medium, and the bottom of the culture dish bottom cover 1 is engraved with scale lines 6 for marking measurements, the zero scale of the scale lines 6 is located at the middle section of the scale lines 6, the scales on the scale lines 6 extend from the middle section to both sides, a positioning hole 7 is opened at the center of the corresponding scale lines 6 in the holding cavity, six scale lines 6 are provided, and the six scale lines 6 are arranged in a circular array with the axis of the culture dish bottom cover 1 as the center, the angle between two scale lines 6 is 60 degrees, and the positioning hole 7 is located at the zero scale position of the scale lines 6.

[0027] By engraving the scale lines 6 at the bottom of the culture dish bottom cover 1, during operation, by pre-processing the bottom of the culture dish bottom cover 1 with auxiliary scales, the size of the inhibition zone can be effectively understood by directly observing the scales at the position of the scale lines 6 when measuring the size of the inhibition zone later, and subsequent measurement by a vernier caliper or the like can be avoided as much as possible, thereby improving the subsequent overall measurement work efficiency and reducing the overall test cost later.

[0028] Among them, the edge of the drug-sensitive paper with the drug is 3 cm away from the axis point of the culture dish bottom cover 1, and the scale 0 point on each scale line 6 is 27 mm away from the axis point of the culture dish bottom cover 1. The scale inward can be made full of 27 mm, and the scale outward can be made to the edge. The overall diameter of the culture dish bottom cover 1 is about 85 mm.

[0029] Reference Figure 1 , Figure 3 and Figure 4 As shown, the bottom of the culture dish bottom cover 1 is protrudingly provided with a protruding seat 2, the bottom of the top cover 8 is set at the top of the protruding seat 2, a plug-in slot 3 is opened between the two scale lines 6 at the bottom of the culture dish bottom cover 1, a plug-in board 4 is installed at the plug-in slot 3, and a receiving slot 42 for placing the corresponding name is opened at the top of the plug-in board 4, and limit strips 31 are symmetrically protruding on both sides of the plug-in slot 3, and limit slots 41 are symmetrically opened on both sides of the plug-in board 4, and the limit strips 31 are plugged in with the limit slots 41.

[0030] By plugging the plug board 4 installed at the plug slot 3 and the receiving slot 42 at the top of the plug board 4, the name of the corresponding drug can be placed in the receiving slot 42 before the drug sensitivity test, which is convenient for observation and subsequent recording. At the same time, it is also avoided as much as possible to directly write the drug name at the bottom of the culture dish bottom cover 1, which requires subsequent secondary cleaning, thereby improving the overall practicality of the culture vessel.

[0031] A non-slip pad 5 is sleeved and installed at the middle section of the outer side edge of the culture dish bottom cover 1, and the outer side edge of the non-slip pad 5 fits with the inner side edge of the top cover 8. The culture dish bottom cover 1, the protruding seat 2 and the top cover 8 are all made of transparent materials. The non-slip pad 5 can be used to assist in sealing the internal space of the culture dish bottom cover 1 and the top cover 8 to ensure that the outside air cannot enter the accommodating cavity during the culture process. By setting each component to be transparent, it can be convenient for observation during the subsequent culture work.

[0032] Working principle:

[0033] During operation, agar is prepared with physiological saline, boiled in water to dissolve, 3.5 ml is sucked with a straw and poured into the receiving cavity of the bottom cover 1 of the culture dish while it is hot to make an agar plate. After the agar solidifies, the paper pieces with the drug names of different test areas are placed in the receiving groove 42 in advance, and the plug-in board 4 is pushed into the plug-in groove 3, so as to name the different experimental areas, and holes are punched at the corresponding positioning holes 7 with a puncher, and the agar in the holes is picked out with a needle, and the drug-sensitive paper pieces with drugs are placed at the position of the positioning holes 7, and the top cover 8 is covered. The anti-slip pad 5 between the bottom cover 1 and the top cover 8 of the culture dish can be used to assist in sealing the internal space of the bottom cover 1 and the top cover 8 of the culture dish, and the culture dish is placed in an incubator for culture, and taken out after a period of time, and the size of the antibacterial circle is observed, and the overall size of the antibacterial circle is effectively determined by the scale line 6 pre-engraved at the bottom of the bottom cover 1 of the culture dish.

[0034] All technical features in this embodiment can be freely combined according to actual needs.

[0035] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A culture vessel for in vitro drug susceptibility testing of digital visualization antibacterial drugs. The culture vessel for in vitro drug susceptibility testing of digital visualization antibacterial drugs includes a culture dish bottom cover (1) and a top cover (8) covering the top of the culture dish bottom cover (1), and is characterized in that, An accommodating cavity for accommodating a medium is provided inside the bottom cover (1) of the petri dish; Scale lines (6) for marking measurements are engraved at the bottom of the bottom cover (1) of the petri dish. The zero scale of the scale lines (6) is located at the middle section of the scale lines (6). The scales on the scale lines (6) extend from the middle section to both sides. A positioning hole (7) is provided at the center corresponding to the scale lines (6) in the accommodating cavity; A plug-in slot (3) is provided at the bottom of the bottom cover (1) of the petri dish corresponding to the space between two scale lines (6). A plug-in board (4) is installed at the plug-in slot (3). An accommodating groove (42) for placing corresponding name indications is provided at the top of the plug-in board (4). Limiting strips (31) are symmetrically protruded on both side edges of the plug-in slot (3). Limiting grooves (41) are symmetrically provided on both side edges of the plug-in board (4). The limiting strips (31) and the limiting grooves (41) are cooperatively plugged together.

2. The culture vessel for in vitro drug susceptibility test of digital visualization antibacterial drugs according to claim 1, characterized in that, Six scale lines (6) are provided, and the six scale lines (6) are arranged in a circular array centered on the axis of the bottom cover (1) of the petri dish.

3. A culture vessel for in vitro drug susceptibility testing of digital visualization antibacterial drugs according to claim 2, characterized in that, The included angle between two scale lines (6) is 60 degrees, and the positioning hole (7) is located at the zero scale position of the scale lines (6).

4. A culture vessel for in vitro drug susceptibility testing of digital visualization antibacterial drugs according to claim 1, characterized in that, A protruding seat (2) is protruded at the bottom of the bottom cover (1) of the petri dish, and the bottom of the top cover (8) abuts against the top of the protruding seat (2).

5. A culture vessel for in vitro drug susceptibility testing of digital visualization antibacterial drugs according to claim 1, characterized in that, An anti-slip pad (5) is sleeved and installed at the middle section of the outer side edge of the bottom cover (1) of the petri dish. The outer side edge of the anti-slip pad (5) is in contact with the inner side edge of the top cover (8). The bottom cover (1) of the petri dish, the protruding seat (2), and the top cover (8) are all made of transparent materials.

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