Rapid detection reagent strip for detecting chlorpyrifos in vegetables
By setting up multiple precoated areas and carboxylated chitosan diaphragm on the test strip, the chlorpyrifos residue detection process is simplified, complex detection problems in the prior art are solved, and fast and simple detection effects are achieved.
Patent Information
- Application Number
- CN202422066808.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-26
- Publication Date
- 2025-07-18
- Estimated Expiration
- 2034-08-26
AI Technical Summary
In the prior art, the residual detection method for chlorpyrifos in vegetable chlorpyrifossil requires professional operation and complex reagent ratios, which is difficult to meet the rapid detection needs of planters.
A test strip including a conjugated antigen precoated area, a monoclonal antibody precoated area, an enzyme-label antibody coated area and a color developer coated area was designed. The reaction process was controlled using a carboxylated chitosan diaphragm, and the operation process was simplified, and independent test strip segments were spliced and tested in the shell.
It realizes fast and simple chlorpyrifos residue detection, which can be completed without professional personnel, reduces detection costs and improves operational convenience.
Smart Images
Figure CN223123026U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to a pesticide residue detection test strip, in particular to a rapid detection reagent strip for chlorpyrifos in vegetables. Background Art
[0002] Chlorpyrifos is a moderately toxic insecticide and highly toxic to aquatic organisms. If used for a long time, it may have long-term adverse effects on the water environment. Chlorpyrifos granules or the method of using poisonous soil can be used to control soil pests such as mole crickets, white grubs, cutworms, vegetable root maggots, and sugarcane tortoise beetles. Spraying chlorpyrifos can control various crop pests, including lepidoptera, coleoptera, homoptera, hemiptera pests, mites, thrips, etc. of food crops, soybeans, peanuts, cotton, fruit trees, vegetables, tea trees, sugarcane, flowers and other crops.
[0003] In the prior art, the main method for rapid detection of chlorpyrifos residues in vegetables is a detection kit based on the indirect competitive ELISA method. However, the detection kit has relatively high requirements for operators. It not only contains many reagents but also requires on-site preparation, and the operation requirements are relatively complex, which is not very convenient for growers to conduct self-detection. Summary of the Utility Model
[0004] The technical problem to be solved by the utility model is to provide a rapid detection reagent strip for chlorpyrifos in vegetables to achieve simple and rapid detection of chlorpyrifos residues.
[0005] The technical solution of the utility model is as follows:
[0006] A rapid detection reagent strip for chlorpyrifos in vegetables includes a test strip, on which an antigen conjugate pre-coated area, a monoclonal antibody pre-coated area, an enzyme-labeled anti-antibody coated area, and a chromogenic agent coated area are sequentially arranged; a first carboxylated chitosan diaphragm is embedded between the antigen conjugate pre-coated area and the monoclonal antibody pre-coated area, a second carboxylated chitosan diaphragm is embedded between the monoclonal antibody pre-coated area and the enzyme-labeled anti-antibody coated area, and a third carboxylated chitosan diaphragm is embedded between the enzyme-labeled anti-antibody coated area and the chromogenic agent coated area.
[0007] Further, the antigen conjugate pre-coated area, the monoclonal antibody pre-coated area, the enzyme-labeled anti-antibody coated area, and the chromogenic agent coated area are independently manufactured test strip segments, which are connected into a test strip by subsequent splicing.
[0008] Further, each test strip segment is spliced by overlapping.
[0009] Further, the first carboxylated chitosan diaphragm, the second carboxylated chitosan diaphragm, and the third carboxylated chitosan diaphragm are all located at the front end of the overlapping position of the corresponding adjacent test strip segments, and the diaphragm is formed by melting carboxylated chitosan and immersing the test strip.
[0010] Further, it further includes a housing, the test strip is located inside the housing, and a sample addition hole and an observation hole are respectively opened on the surface of the housing corresponding to the coupling antigen pre-coated area and the coupling antigen pre-coated area.
[0011] Further, the housing is made of PC material.
[0012] The beneficial effects of the present utility model are as follows:
[0013] The advantage of the present utility model is that the detection kit that used to require complicated and professional operations in the past has been improved into a rapid detection reagent strip. The corresponding reagents are pre-coated on the reagent strip in sequence according to the reaction sequence, and then controlled by carboxylated chitosan diaphragms with different thicknesses according to the reaction time, and the reagent amounts in each reaction area are pre-matched, so that rapid detection can be carried out as soon as one starts, without the need for professional operators to use it. BRIEF DESCRIPTION OF THE DRAWINGS
[0014] Figure 1 It is a schematic structural diagram of the present utility model.
[0015] In the figure: 1 - housing, 11 - sample addition hole, 12 - observation hole, 2 - test strip, 21 - coupling antigen pre-coated area, 22 - monoclonal antibody pre-coated area, 23 - enzyme-labeled anti-antibody coated area, 24 - chromogenic agent coated area, 3 - first carboxylated chitosan diaphragm, 31 - second carboxylated chitosan diaphragm, 32 - third carboxylated chitosan diaphragm. DETAILED DESCRIPTION OF THE EMBODIMENTS
[0016] The following further describes the specific embodiments of the present utility model with reference to the drawings. It should be noted here that the description of these embodiments is for helping to understand the present utility model, but does not constitute a limitation to the present utility model. In addition, the technical features involved in the various embodiments of the present utility model described below can be combined with each other as long as they do not conflict with each other.
[0017] As Figure 1 shown:
[0018] A rapid detection reagent strip for detecting chlorpyrifos in vegetables includes a test strip 2, and a coupling antigen pre-coated area 21, a monoclonal antibody pre-coated area 22, an enzyme-labeled anti-antibody coated area 23, and a chromogenic agent coated area 24 are sequentially arranged on the test strip 2; a first carboxylated chitosan diaphragm 3 is embedded between the coupling antigen pre-coated area 21 and the monoclonal antibody pre-coated area 22, a second carboxylated chitosan diaphragm 31 is embedded between the monoclonal antibody pre-coated area 22 and the enzyme-labeled anti-antibody coated area 23, and a third carboxylated chitosan diaphragm 32 is embedded between the enzyme-labeled anti-antibody coated area 23 and the chromogenic agent coated area 24.
[0019] In the present utility model, the chlorpyrifos conjugate antigen used in the conjugate antigen pre-coated region 21 is obtained by conjugating a chlorpyrifos hapten with a carrier protein. The chlorpyrifos monoclonal antibody used in the monoclonal antibody pre-coated region 22 is prepared using the chlorpyrifos conjugate antigen as an immunogen. The labeling enzyme of the enzyme-labeled anti-antibody is horseradish peroxidase, and the chromogenic agent is a 1:1 mixture of urea peroxide and o-phenylenediamine. The above are existing test reagents for the indirect competitive ELISA method, and the preparation method is a well-known technology.
[0020] The advantages of the present utility model are that the detection kit that previously required complicated and professional operations has been improved into a rapid detection test strip. Corresponding reagents are pre-coated on the test strip 2 in sequence according to the reaction sequence, and then, according to the reaction time, it is controlled by carboxylated chitosan diaphragms with different thicknesses. The reagent amounts in each reaction region are pre-matched, and rapid detection can be carried out as soon as one starts, without the need for professional operators to use it.
[0021] Since pre-coating is mostly carried out by the method of solution impregnation and drying, the conjugate antigen pre-coated region 21, the monoclonal antibody pre-coated region 22, the enzyme-labeled anti-antibody coated region 23, and the chromogenic agent coated region 24 are independent test paper segments made. This can reduce costs. After being made separately and cut, they are connected into the test strip 2 by subsequent splicing. Specifically, the test paper segments can be spliced by the way of overlapping. The first carboxylated chitosan diaphragm 3, the second carboxylated chitosan diaphragm 31, and the second carboxylated chitosan diaphragm 31 are all located at the front end of the overlapping position of the corresponding adjacent test paper segments, and the carboxylated chitosan is melted and immersed in the test strip 2 to form a diaphragm.
[0022] As an optimized scheme, it further includes a housing 1. The test strip 2 is located inside the housing 1. Sampling holes 11 and observation holes 12 are respectively opened on the surface of the housing 1 corresponding to the conjugate antigen pre-coated region 21 and the conjugate antigen pre-coated region 21. The housing 1 can be made of PC material, which can avoid the contamination and loss of the pre-coated reagents during the packaging and use process. The test sample to be detected is dropped into from the sampling hole 11. After the sample is mixed with the conjugate antigen, chlorpyrifos in the sample competes with the chlorpyrifos conjugate antigen for the chlorpyrifos monoclonal antibody, and then is amplified by the enzyme-labeled anti-antibody. The chromogenic agent coated region 24 shows color. The color change is viewed from the observation hole 12. The color depth is related to the chlorpyrifos concentration, and the concentration range can be known by referring to the standard colorimetric card.
[0023] The above has described in detail the embodiments of the present utility model in conjunction with the accompanying drawings, but the present utility model is not limited to the described embodiments. For those skilled in the art, without departing from the principles and spirits of the present utility model, various changes, modifications, substitutions, and variations made to these embodiments still fall within the protection scope of the present utility model.
Claims
1. A rapid detection test strip for chlorpyrifos in vegetables, characterized in that: It includes a test strip, on which an antigen-conjugate pre-coated area, a monoclonal antibody pre-coated area, an enzyme-labeled anti-antibody coated area, and a chromogenic agent coated area are sequentially arranged; a first carboxylated chitosan diaphragm is embedded between the antigen-conjugate pre-coated area and the monoclonal antibody pre-coated area, a second carboxylated chitosan diaphragm is embedded between the monoclonal antibody pre-coated area and the enzyme-labeled anti-antibody coated area, and a third carboxylated chitosan diaphragm is embedded between the enzyme-labeled anti-antibody coated area and the chromogenic agent coated area.
2. The rapid detection reagent strip for chlorpyrifos in vegetables according to claim 1, wherein: The antigen-conjugate pre-coated area, the monoclonal antibody pre-coated area, the enzyme-labeled anti-antibody coated area, and the chromogenic agent coated area are independently produced test paper segments, which are connected into a test strip by subsequent splicing.
3. The rapid detection reagent strip for chlorpyrifos in vegetables according to claim 2, characterized in that: Each test paper segment is spliced by overlapping.
4. The rapid detection reagent strip for chlorpyrifos in vegetables according to claim 1, wherein: The first carboxylated chitosan diaphragm, the second carboxylated chitosan diaphragm, and the third carboxylated chitosan diaphragm are all located at the front end of the overlapping position of the corresponding adjacent test paper segments, and the diaphragm is formed by melting carboxylated chitosan and then immersing the test strip.
5. The rapid detection reagent strip for chlorpyrifos in vegetables according to claim 1, characterized in that: It further includes a housing, the test strip is located inside the housing, and a sample adding hole and an observation hole are respectively opened on the surface of the housing corresponding to the antigen-conjugate pre-coated area and the antigen-conjugate pre-coated area.
6. The rapid detection reagent strip for chlorpyrifos in vegetables according to claim 5, characterized in that: The housing is made of PC material.