Three-dimensional optical imaging system

Abstract

The utility model relates to the technical field of biological imaging, in particular to a three-dimensional optical imaging system. The imaging unit and the projector are installed in an inner cavity of a box body side by side, the LED light source and the object carrying unit are sequentially installed in the inner cavity of the box body along an imaging path of the imaging unit, the LED light source comprises at least two lamp caps distributed on the periphery of the imaging path in the circumferential direction, and irradiation light of the lamp caps obliquely points to the object carrying unit. And the irradiation areas of all the lamp caps on the object carrying unit are overlapped and all cover the imaging view field. According to the multi-modal imaging device, multi-modal imaging of biological optics can be achieved, the overall layout of the device is more reasonable, and better adaptability is achieved in the image acquisition process of molecular fluorescence imaging.

Description

Technical Field

[0001] The utility model relates to the technical field of bio-imaging, specifically a three-dimensional optical imaging system. Background Art

[0002] According to different detection methods of bio-optical imaging, bio-optical imaging can be divided into molecular fluorescence imaging, bioluminescence imaging, photoacoustic imaging, optical tomography imaging, etc.

[0003] In the three-dimensional imaging technology of organisms, there is a three-dimensional imaging system formed by combining multiple bio-optical imaging methods. In this regard, the applicant has applied for a patent text with the title of "A 3D Optical Imaging System and Method" and the Chinese patent publication number of CN117379007B. The content is as follows: By using the same CCD camera to obtain two-dimensional bio-optical images and three-dimensional surface contour images of the imaging target, and then registering the two-dimensional bio-optical images and three-dimensional surface contour images; using biological tissues with different optical properties to fill the three-dimensional surface contour images and combining the registered data, three-dimensional bio-optical images can be obtained, realizing the three-dimensional spatial distribution of bio-optical signals in organisms. The technical solution of the above application has achieved excellent three-dimensional imaging effects. Therefore, on the basis of the above application, the applicant also considers further researching and developing an improved solution, not only making the overall layout of the device more reasonable, but also having better adaptability in the image acquisition process of molecular fluorescence imaging, so it needs to be solved urgently. Summary of the Utility Model

[0004] In order to avoid and overcome the technical problems existing in the prior art, the utility model provides a three-dimensional optical imaging system, which can realize multi-modal imaging of bio-optics, and the overall layout of the device is more reasonable, and it has better adaptability in the image acquisition process of molecular fluorescence imaging.

[0005] To achieve the above object, the utility model provides the following technical solutions:

[0006] A three-dimensional optical imaging system includes an imaging unit and a projector arranged side by side in the inner cavity of a box, and further includes an LED light source and a loading unit arranged in the inner cavity of the box in sequence along the imaging path of the imaging unit. The LED light source includes at least two lamp heads circumferentially distributed on the outer periphery of the imaging path. The irradiation light of the lamp heads is inclined towards the loading unit, and the irradiation areas of all the lamp heads on the loading unit coincide and all cover the imaging field of view.

[0007] As a further solution of the utility model: all the lamp heads are circumferentially evenly distributed on the outer periphery of the imaging path, the axes of all the lamp heads enclose a conical structure converging towards the loading unit, and the irradiation light of all the lamp heads covers at least two wavelengths.

[0008] As a further solution of the present utility model: A light-shielding cover is fixed inside the box body. The open end of the light-shielding cover is fixedly sealed with the end wall of the box body near one end of the imaging unit, and a heat dissipation hole communicating with the light-shielding cover is opened on this end wall. The imaging unit includes a CCD camera. Both the CCD camera and the projector are fixed inside the light-shielding cover. A through hole for the imaging lens of the CCD camera to penetrate and a light-transmitting hole for the projection lens of the projector to penetrate are opened on the closed end of the light-shielding cover.

[0009] As a further solution of the present utility model: The loading unit is separately separated in the inner cavity of the box body by a partition. The partition is provided with a first through hole and a second through hole respectively for the light emitted by the projector and the LED light source to pass through. A supplementary light lamp is installed on the side wall of the partition close to the loading unit, and the supplementary light lamps are provided with at least two which are circumferentially uniformly distributed on the outer periphery of the second through hole, and the supplementary light lamps are white light lamps inclined towards the loading unit.

[0010] As a further solution of the present utility model: The inner wall of the box body, the partition and the surface wall of the light-shielding cover are all of a dark-colored rough surface structure.

[0011] As a further solution of the present utility model: The imaging unit further includes a filter assembly. The filter assembly is located between the front end of the CCD camera lens and the LED light source. The filter assembly includes a plurality of filter plates that can be respectively and actively switched to the front end of the CCD camera lens.

[0012] As a further solution of the present utility model: A laser generator for positioning the position of the organism is installed on the partition.

[0013] As a further solution of the present utility model: The loading unit includes a loading table. The box body is provided with a lifting assembly and a rotating assembly that respectively drive the loading table to perform lifting motion and rotary motion around the vertical axis.

[0014] As a further solution of the present utility model: The loading unit further includes a fixing plate and a sample holder installed on the fixing plate. A slot is provided at the top of the loading table. A plug block that forms a plug-in and anti-rotation fit with the slot is provided at the bottom of the fixing plate. A plurality of mounting grooves of different specifications are provided at the top of the fixing plate for plugging and fixing sample holders of different specifications.

[0015] As a further solution of the present utility model: An anesthetic nozzle is installed on the sample holder. The nozzle of the anesthetic nozzle points to the sample fixing working surface on the sample holder. An anesthetic supply assembly connected to the anesthetic nozzle is installed on the box wall of the box body.

[0016] Compared with the prior art, the beneficial effects of the present utility model are:

[0017] 1. The three-dimensional imaging system can not only achieve multiple three-dimensional imaging methods that integrate structured light with molecular fluorescence imaging or bioluminescence, but also has a more reasonable overall layout. In particular, by using an LED light source as the excitation light source for molecular fluorescence imaging, compared with the traditional laser excitation method, the signal-to-noise ratio is improved, which not only effectively reduces the overall cost of the imaging system, but also because the irradiation areas of all lamp heads on the loading unit overlap and all cover the imaging field of view. In actual implementation, the irradiation light of at least two lamp heads can be set to cover at least two wavelengths, so that when there are different requirements for the light wavelength of the laser light source, lamp heads that can emit irradiation light of the corresponding wavelength can be selected for irradiation to achieve the best imaging environment, and thus have better adaptability during the excitation process of molecular fluorescence imaging.

[0018] 2. All lamp heads are circumferentially evenly distributed on the outer periphery of the imaging path, and the axes of all lamp heads enclose a conical structure converging towards the loading unit, so that the irradiation distances of all lamp heads on the loading unit are the same. Therefore, in actual implementation, the irradiation light wavelengths of all lamp heads or some of them can be set to be different from each other and cover the wavelength range of the required excitation light source. That is, when there are different requirements for the wavelength of the excitation light source, the corresponding wavelength of irradiation light can be directly emitted by the lamp head that meets this requirement.

[0019] 3. A light shield is fixed inside the box body, and both the CCD camera and the projector are fixed inside the light shield. The closed end of the light shield is provided with a through hole for the imaging lens of the CCD camera to penetrate and a light-transmitting hole for the projection lens of the projector to penetrate, so as not to block the CCD camera from collecting images and not to hinder the projector from projecting light onto the loading unit. In addition, the open end of the light shield is hermetically fixed to the end wall of the box body near the imaging unit, and a heat dissipation hole communicating with the light shield is provided on this end wall, so that the light entering the inner cavity of the box body from the heat dissipation hole can only directly enter the light shield; but since the imaging lens of the camera and the projection lens of the projector penetrate to the outside of the light shield, the light entering the light shield will not interfere with the projection lens of the projector and the imaging lens of the CCD camera. Thus, the heat dissipation of the CCD camera and the projector is achieved, and the use of the CCD camera and the projector will not be interfered.

[0020] 4. A fill light is provided. In the structured light imaging mode, the fill light emits diffuse reflection light uniformly to the loading unit, which can ensure the uniformity of light, reduce the sensitivity of the camera to light, and improve the imaging quality.

[0021] 5. The inner wall of the box body, the partition board, and the surface wall of the light shield are all of dark-colored rough surface structure, which can reduce the reflection of light in the inner cavity of the box body, and thus reduce the influence of the reflected light on the three-dimensional imaging effect.

[0022] 6. The imaging unit includes a filter assembly, which is located between the front end of the CCD camera lens and the LED light source. The filter assembly includes a plurality of filter plates that can be respectively and actively switched to the front end of the CCD camera lens, and different filter plates can be adjusted and replaced according to the requirements of biological optical imaging for filtering operations.

[0023] 7. A laser generator for positioning the position of the organism is installed on the partition board, and the center point of the organism to be imaged is determined by the intersection of the XYZ axes of the laser, the lens of the CCD camera, and the structured light of the projector.

[0024] 8. The stage of the loading unit can be respectively driven by a lifting assembly and a rotating assembly to perform lifting motion and rotary motion, so as to quickly adjust the position and angle of the organism to be imaged.

[0025] 9. The sample bracket adopts a quick-release structure with plug-in connection, and the installation is fast and convenient.

[0026] 10. Anesthesia nozzles are arranged on the sample bracket to facilitate anesthesia of the organism to be imaged. Description of the Drawings

[0027] Figure 1 It is the first three-dimensional structure schematic diagram of the present utility model.

[0028] Figure 2 It is the second three-dimensional structure schematic diagram of the present utility model.

[0029] Figure 3 It is the structure schematic diagram of the embodiment of the present utility model including a light-shielding cover.

[0030] Figure 4 It is the left view structure schematic diagram of the present utility model.

[0031] Figure 5 It is the structure schematic diagram of the loading unit in the present utility model.

[0032] Figure 6 It is the structure schematic diagram of the fixed disk in the present utility model.

[0033] In the figure: 10, box body; 11, partition board; 111, first through hole; 112, second through hole; 20, imaging unit; 21, CCD camera; 22, filter assembly; 30, LED light source; 31, lamp holder; 40, loading unit; 41, lifting assembly; 42, rotating assembly; 43, stage; 431, slot; 44, fixed disk; 441, insertion block; 442, installation groove; 45, sample bracket; 451, anesthesia nozzle; 50, projector; 60, supplementary light; 70, laser generator; 80, light-shielding cover; 81, through hole; 82, light-transmitting hole; 90, anesthesia supply assembly. Detailed Embodiment

[0034] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0035] For ease of understanding, the specific structure and working mode of the present invention will be further described below with reference to the accompanying drawings:

[0036] The specific structure of the present invention is referred to Figures 1-6 As shown, its main structure includes an imaging unit 20 and a projector 50 that are installed side by side in the inner cavity of the box body 10, and also includes an LED light source 30 and a loading unit 40 that are sequentially installed in the inner cavity of the box body 10 along the imaging path of the imaging unit 20. Specifically, the loading unit 40 is used to fix the organism to be imaged; the LED light source 30 serves as an excitation light source in the molecular fluorescence imaging mode, and is used to excite the fluorescence molecules carried by the organism to generate emitted fluorescence. Among them, the LED light source 30 includes at least two lamp heads 31 that are circumferentially distributed on the outer periphery of the imaging path. The irradiation lights of the projector 50 and the lamp heads 31 are all inclined towards the loading unit 40, and the irradiation areas of all the lamp heads 31 on the loading unit 40 coincide and all cover the imaging field of view; the projector 50 serves as a structured light source in the structured light three-dimensional imaging mode, and the irradiation light of the projector 50 is inclined towards the loading unit 40, and is used to irradiate the structured light on the organism fixed on the loading unit 40; in addition, in the bioluminescence imaging mode, an enzymatic reaction occurs inside the organism of the imaging object, so that the autofluorescence in the animal body is provided by the small animal itself as a bioluminescence light source; the imaging unit 20 is used to collect the acquired images in the above molecular fluorescence imaging mode, structured light three-dimensional imaging mode or bioluminescence imaging mode.

[0037] Through the above structural layout, not only can the three-dimensional imaging system achieve a three-dimensional imaging method of molecular fluorescence imaging, bioluminescence imaging and structured light fusion imaging, but also the overall layout is more reasonable. Especially, by using the LED light source 30 as the excitation light source for molecular fluorescence imaging, compared with the traditional laser excitation method, not only the overall cost of the imaging system is effectively reduced, but also because the irradiation areas of all the lamp heads 31 on the loading unit 40 coincide, in actual implementation, the irradiation light wavelengths of two lamp heads 31 can be set differently, so that when different requirements for the light wavelength of the laser light source exist, the lamp heads 31 with different irradiation light wavelengths can be selected for irradiation to achieve the best imaging environment, and thus better adaptability can be achieved during the excitation process of molecular fluorescence imaging.

[0038] Specifically, as Figure 1As shown, all the lamp holders 31 are circumferentially arranged evenly on the outer periphery of the imaging path. The axes of all the lamp holders 31 enclose a conical structure converging towards the loading unit 40, so that the irradiation distances of all the lamp holders 31 on the loading unit 40 are the same. Thus, in actual implementation, the irradiation light wavelengths of all the lamp holders 31 can be set to be different and cover the wavelength range of the required excitation light source. That is, when there are different requirements for the wavelength of the excitation light source, the irradiation light of the corresponding wavelength can be directly emitted by the lamp holder 31 that is the same as the requirement. Of course, it is also possible to only set the irradiation light wavelengths of some of the lamp holders 31 to be different. When the brightness of a single lamp holder 31 is not sufficient to meet the excitation conditions of the fluorescent molecules, the excitation effect of the fluorescent molecules can be ensured by turning on multiple lamp holders 31 with the same irradiation wavelength.

[0039] To reduce the interference of external light, the imaging system is often used in a dark environment sealed in the inner cavity of the box body 10. During actual use, it often causes the heat of the imaging unit 20 and the projector 50 to not dissipate better to the outside. Therefore, to ensure the good imaging effect of the imaging system and ensure good heat dissipation for the imaging unit 20 and the projector 50, the inner cavity structure of the box body 10 is further optimized. Specifically, as Figure 3 shown, a light-shielding cover 80 is fixed inside the box body 10. The imaging unit 20 includes a CCD camera 21, and both the CCD camera 21 and the projector 50 are fixed inside the light-shielding cover 80; as Figure 4 shown, the closed end of the light-shielding cover 80 is provided with a through hole 81 for the imaging lens of the CCD camera 21 to penetrate and a light-transmitting hole 82 for the projection lens of the projector 50 to penetrate, so as not to block the CCD camera 21 from collecting images and not hinder the projector 50 from projecting light onto the loading unit 40; in addition, the open end of the light-shielding cover 80 is fixedly sealed with the end wall of the box body 10 near the imaging unit 20, and a heat dissipation hole communicating with the light-shielding cover 80 is opened on this end wall. Thus, the light entering the inner cavity of the box body 10 from the heat dissipation hole can only directly enter the light-shielding cover 80. However, since the imaging lens of the CCD camera 21 and the projection lens of the projector 50 penetrate to the outside of the light-shielding cover 80, the light entering the light-shielding cover 80 will not interfere with the projection lens of the projector 50 and the imaging lens of the CCD camera 21. Thus, heat dissipation for the CCD camera 21 and the projector 50 is achieved, and it will not cause interference to the use of the CCD camera 21 and the projector 50.

[0040] On the above basis, as Figures 1-3As shown in the figure, the loading unit 40 is separately partitioned in the inner cavity of the box body 10 by the partition plate 11, which is used to divide the inner cavity of the box body 10 into an illumination imaging area and a loading area. The partition plate 11 is provided with a first through hole 111 and a second through hole 112 for the light emitted by the projector 50 and the LED light source 30 to pass through respectively. In addition, a supplementary light 60 is installed on the side wall of the partition plate 11 close to the loading unit 40. The supplementary light 60 is a white light lamp inclined towards the loading unit 40, and at least two supplementary lights 60 are arranged circumferentially and uniformly around the outer periphery of the second through hole 112 to achieve uniform supplementary lighting for the imaging area of the loading unit 40. Preferably, the white light lamp is a diffuse reflection lamp. In the structured light imaging mode, the supplementary light 60 emits diffuse reflection light uniformly to the loading unit 40, which can ensure the uniformity of light, reduce the sensitivity of the camera to light, and improve the imaging quality. Of course, it should be noted that when the supplementary light 60 is turned on, no filter is required to be set in front of the lens of the CCD camera 21.

[0041] In addition, the inner wall of the box body 10, the partition plate 11 and the surface wall of the light-shielding cover 80 are all of a dark-colored rough surface structure, which can reduce the reflection of light in the inner cavity of the box body 10, and further reduce the influence of the reflected light on the three-dimensional imaging effect.

[0042] On the above basis, as Figure 2 shown, the imaging unit 20 further includes a filter component 22. The filter component 22 is located between the front end of the lens of the CCD camera 21 and the LED light source 30. The filter component 22 includes a plurality of filters that can be respectively switched to the front end of the lens of the CCD camera 21, and different filters can be adjusted and replaced according to the requirements of biological optical imaging for filtering operations.

[0043] In addition, as Figure 1 shown, a laser generator 70 for positioning the position of the organism is installed on the partition plate 11. In actual implementation, a plurality of laser generators 70 can be set, and the intersection points of the XYZ axes of the laser, the lens of the CCD camera 21, and the structured light of the projector 50 are used to determine the center point of the imaged organism.

[0044] As Figure 5 shown, the loading unit 40 includes a loading platform 43. The box body 10 is equipped with a lifting component 41 and a rotating component 42 that respectively drive the loading platform 43 to move up and down and rotate around the vertical axis, so that the fixed position and fixed angle of the organism to be imaged can be adjusted according to actual needs. In actual implementation, the lifting component 41 preferably adopts a ball screw structure, and a lifting mechanism such as a cylinder or a hydraulic cylinder can also be used; the rotating component 42 preferably adopts a stepping motor or a servo motor.

[0045] To facilitate the rapid fixation of the biological sample to be imaged, as Figure 3 shown, the loading unit 40 further includes a fixing plate 44 and a sample holder 45 installed on the fixing plate 44. Specifically, as Figure 5As shown, a slot 431 is provided at the top of the stage 43. As Figure 6 shown, a plug 441 that forms a plug-in and anti-rotation fit with the slot 431 is provided at the bottom of the fixed disk 44 for the quick disassembly and assembly between the fixed disk 44 and the stage 43. In addition, a number of mounting grooves 442 of different specifications are provided at the top of the fixed disk 44 for plugging and fixing sample holders 45 of different specifications, so that different sample holders 45 can be selected according to the to-be-imaged organisms of different external dimensions and quickly fixed and installed with the fixed disk 44.

[0046] Furthermore, as Figure 3 shown, an anesthetic nozzle 451 is installed on the sample holder 45, and the nozzle of the anesthetic nozzle 451 points to the sample fixing working surface on the sample holder 45. An anesthetic supply assembly 90 connected to the anesthetic nozzle 451 is installed on the box wall of the box body 10 to facilitate quickly anesthetizing the to-be-imaged organisms on the sample holder 45.

[0047] To facilitate further understanding of the technical solution of the present invention, the working principles of the above-mentioned bioluminescence imaging, molecular fluorescence imaging, and structured light three-dimensional imaging are respectively elaborated as follows:

[0048] After fixing the experimental animal on the loading unit 40 at the required mounting height and mounting angle, the imaging operation can be started.

[0049] In the molecular fluorescence imaging mode, the projector 50 is turned off. According to the wavelength of the required excitation light source, the LED light source 30 lamp head 31 with the irradiation light wavelength corresponding to this wavelength is turned on and irradiated on the to-be-imaged organisms on the loading unit 40 to excite the fluorescence molecules carried by the to-be-imaged organisms to generate emitted fluorescence. The fluorescence light passes through the second through hole 112 and after being processed by the filter, the CCD camera 21 with a lens collects the molecular fluorescence images. The rotating assembly 42 drives the to-be-imaged organisms to rotate, and the CCD camera 21 collects multiple molecular fluorescence images at a certain frequency. Finally, molecular fluorescence imaging is performed through data processing;

[0050] In the bioluminescence imaging mode, the chemical reactions inside the imaging object come from enzymatic reactions in the living organism, which are the autofluorescence in the animal body. The enzyme that catalyzes such reactions is called luciferase. The common method is to construct an expression vector of the luciferase gene, transfect the target cells, and transplant them into the target organ of the recipient. When observing, an exogenous luciferin is injected, and a reaction occurs in the target cells to produce fluorescence. Then, a highly sensitive in-vivo bioluminescence imaging system can be used to achieve real-time monitoring of the expression of target cells or target molecules; therefore, there is no need for the participation of the LED light source 30 and the projector 50, and the light generated by the luminescent substances (such as luciferase) in the living organism is used for imaging. After that, the light passes through the second through hole 112, and after being processed by the filter, the CCD camera 21 with a lens collects the bioluminescence image. The rotating assembly 42 drives the organism to be imaged to rotate, and the CCD camera 21 collects multiple bioluminescence images at a certain frequency, and finally performs bioluminescence imaging through data processing;

[0051] The structured light three-dimensional imaging mode is combined with the molecular fluorescence imaging mode or the bioluminescence imaging mode for acquisition and imaging. Usually, image acquisition will be carried out during or after the acquisition of images in the molecular fluorescence imaging mode or the bioluminescence imaging mode. Specifically, after the molecular fluorescence is excited or during the bioluminescence process, the CCD camera is used to first capture the two-dimensional bioluminescence or molecular fluorescence image, and then the projector 50 projects the pre-modulated structured light. The structured light passes through the first through hole 111 and irradiates the surface of the organism to be imaged on the loading unit 40. During the acquisition process, the rotating assembly 42 drives the organism to be imaged to rotate, and the CCD camera 21 completes the acquisition of the three-dimensional surface image at a certain frequency, and performs registration processing based on the acquired three-dimensional surface image data and the two-dimensional molecular fluorescence imaging or bioluminescence image to achieve three-dimensional contour reconstruction.

[0052] Certainly, for those skilled in the art, the present utility model is not limited to the details of the above exemplary embodiments, but also includes the same or similar structures that can be implemented in other specific forms without departing from the spirit or basic characteristics of the present utility model. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting. The scope of the present utility model is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the present utility model. Any reference signs in the claims should not be regarded as limiting the claimed rights.

[0053] In addition, it should be understood that although this specification is described according to the embodiments, not every embodiment only contains an independent technical solution. This narrative way of the specification is only for clarity. Those skilled in the art should regard the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

[0054] The technologies, shapes, and structures not described in detail in this utility model are all well-known technologies.

Claims

1. A three-dimensional optical imaging system, characterized in that, It includes an imaging unit (20) and a projector (50) installed side by side in the inner cavity of a box body (10), and also includes an LED light source (30) and a loading unit (40) installed in sequence along the imaging path of the imaging unit (20) in the inner cavity of the box body (10). The LED light source (30) includes at least two lamp heads (31) circumferentially distributed on the outer periphery of the imaging path. The irradiation light of the lamp heads (31) is inclined towards the loading unit (40), and the irradiation areas of all the lamp heads (31) on the loading unit (40) coincide and all cover the imaging field of view.

2. The three-dimensional optical imaging system according to claim 1, wherein All the lamp heads (31) are circumferentially and evenly distributed on the outer periphery of the imaging path. The axes of all the lamp heads (31) enclose a conical structure converging towards the loading unit (40), and the irradiation light of all the lamp heads (31) covers at least two wavelengths.

3. The three-dimensional optical imaging system according to claim 1 or 2, characterized in that, A light-shielding cover (80) is fixed inside the box body (10). The open end of the light-shielding cover (80) is hermetically fixed to the end wall of the box body (10) near one end of the imaging unit (20), and a heat dissipation hole communicating with the light-shielding cover (80) is opened on this end wall. The imaging unit (20) includes a CCD camera (21). Both the CCD camera (21) and the projector (50) are fixed inside the light-shielding cover (80). A through hole (81) for the imaging lens of the CCD camera (21) to penetrate and a light-transmitting hole (82) for the projection lens of the projector (50) to penetrate are opened on the closed end of the light-shielding cover (80).

4. The three-dimensional optical imaging system according to claim 3, wherein The loading unit (40) is separately separated in the inner cavity of the box body (10) by a partition plate (11). The partition plate (11) is provided with a first through hole (111) and a second through hole (112) respectively for the light emitted by the projector (50) and the LED light source (30) to pass through. A supplementary light lamp (60) is installed on the side wall of the partition plate (11) close to the loading unit (40), and the supplementary light lamp (60) is set to be at least two circumferentially and evenly distributed on the outer periphery of the second through hole (112), and the supplementary light lamp (60) is a white light lamp inclined towards the loading unit (40).

5. The three-dimensional optical imaging system according to claim 4, wherein The inner wall of the box body (10), the partition plate (11) and the surface wall of the light-shielding cover (80) are all of a dark-colored rough surface structure.

6. The three-dimensional optical imaging system according to claim 4, wherein The imaging unit (20) further includes a filter assembly (22). The filter assembly (22) is located between the front end of the lens of the CCD camera (21) and the LED light source (30). The filter assembly (22) includes a plurality of filter plates that can be respectively and movably switched to the front end of the lens of the CCD camera (21).

7. The three-dimensional optical imaging system according to claim 4, wherein A laser generator (70) for positioning the position of the organism is installed on the partition plate (11).

8. The three-dimensional optical imaging system according to claim 1 or 2, characterized in that, The loading unit (40) includes a loading platform (43). A lifting assembly (41) and a rotating assembly (42) for respectively driving the loading platform (43) to perform lifting motion and rotational motion around the vertical axis are installed on the box body (10).

9. The three-dimensional optical imaging system according to claim 8, characterized in that, The loading unit (40) further includes a fixing plate (44) and a sample holder (45) mounted on the fixing plate (44). A slot (431) is provided at the top of the stage (43). A plug (441) is provided at the bottom of the fixing plate (44) to form a plug-in and anti-rotation fit with the slot (431). A number of mounting grooves (442) of different specifications are provided at the top of the fixing plate (44) for plugging and fixing sample holders (45) of different specifications.

10. The three-dimensional optical imaging system according to claim 9, characterized in that, An anesthetic nozzle (451) is mounted on the sample holder (45). The nozzle of the anesthetic nozzle (451) points to the sample fixing working surface on the sample holder (45). An anesthetic supply assembly (90) connected to the anesthetic nozzle (451) is mounted on the box wall of the box body (10).

Images