Test tube fixing mechanism and fluorescent quantitative PCR detection kit

Abstract

The utility model relates to the technical field of kits, in particular to a test tube fixing mechanism and a fluorescent quantitative PCR (Polymerase Chain Reaction) detection kit, which comprise a test tube seat, a test tube groove and a stabilizing component, two groups of handles are symmetrically arranged on the test tube seat; a mounting cavity is formed in one side of the test tube groove and located on the test tube seat; the stabilizing assembly is composed of a bearing unit and an abutting unit, the bearing unit is arranged at the bottom end in the test tube groove, and the abutting unit is mounted in the mounting cavity. The test tubes with different sizes and filled with reagents can be fixedly installed in the test tube grooves through the stabilizing assemblies, the test tubes are prevented from being impacted in the transfer process, the application range is wide, and the installation stability of the test tubes is improved; through the arrangement of a limiting seat, a handle and a first spring, the test tube seat fixedly provided with a plurality of groups of test tubes can be conveniently and stably mounted in the kit, the internal placement space of the kit is reasonably planned, the test tubes are prevented from being collided and leaked in the transfer process, the test tube mounting stability is further improved, and the reagent placement stability is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of reagent kits, in particular to a test tube fixing mechanism and a fluorescence quantitative PCR detection kit. Background Art

[0002] Real-time fluorescence quantitative PCR is a method that uses fluorescent chemicals to measure the total amount of products after each polymerase chain reaction (PCR) cycle in a DNA amplification reaction. It is a method for quantitatively analyzing specific DNA sequences in the sample to be tested by using internal or external reference methods. The detection of fluorescent quantitative PCR requires that the test tube with the sample be placed in the kit to facilitate the reaction of the sample and complete the detection.

[0003] The Chinese patent with the authorization announcement number CN220683329U discloses a fluorescent quantitative PCR detection kit, which belongs to the technical field of kits, including a protective box, a box cover is hinged on the top of the protective box, a storage module is snap-connected at the lower end of the box cover, the protective box is snap-connected at the bottom of the storage module, an isolation sleeve is fixedly connected inside the protective box, and a storage box is slidably connected inside the protective box. The utility model drives the rotating rod to rotate by starting the motor, and drives the storage block to rotate by the rotation of the rotating rod. The storage block will drive the storage box to rotate, so that different storage blocks correspond to the bottom of the isolation cover respectively. When the storage block is located below the isolation cover, the telescopic rod inside the storage hole corresponding to the lower end of the opened isolation cover will push out the reagent storage tube, so as to take out the reagent storage tube. In the process of taking out the reagent storage tube, it will not interfere with other reagent storage tubes located inside the storage hole, and will not cause contamination to the reagent.

[0004] However, the above-mentioned disclosed solution has the following shortcomings: the reagent storage tube is not fixed in the storage hole, and once the protective box collides, the test tube is easily damaged, which may easily cause the reagent to spill, and the stability is low, making it inconvenient to transport the test tube, which brings inconvenience to use. Utility Model Content

[0005] The utility model aims to solve the problems existing in the background technology and propose a test tube fixing mechanism and a fluorescence quantitative PCR detection kit.

[0006] The technical solution of the utility model is as follows: a test tube fixing mechanism comprises a test tube seat, on which two groups of handles are symmetrically arranged; a test tube slot, on which a plurality of groups of test tube slots are evenly distributed inside the test tube seat, a mounting cavity is arranged on one side of the test tube slot and located on the test tube seat; and a stabilizing component, which is composed of a receiving unit and an abutting unit, the receiving unit is arranged at the bottom end of the test tube slot and is used to abut against the bottom end of the test tube, and the abutting unit is installed in the mounting cavity and is used to detachably connect the top end of the test tube with the receiving unit.

[0007] Preferably, the receiving unit includes a second spring installed at the inner bottom end of the test tube slot, and a base installed at the top end of the second spring. The base slides and adjusts along the height direction of the test tube holder, and a groove is formed at the top end of the base.

[0008] Preferably, multiple groups of sliders are evenly arranged on the side wall of the base, and multiple groups of guiding grooves are evenly formed on the side wall of the test tube slot and on the test tube holder. The other end of the slider is slidably connected to the corresponding guiding groove.

[0009] Preferably, the abutting unit includes a screw rod installed at the inner bottom end of the installation cavity, an adjusting tube slidably connected to the installation cavity and threadedly connected to the outer wall of the screw rod, and an abutting cover. One end of the abutting cover is rotatably connected to the upper end of the outer wall of the adjusting tube, and a rubber pad is arranged at the bottom of the other end.

[0010] A fluorescence quantitative PCR detection kit includes a box body, one side of the top end of which is hingedly connected with a box cover, and the other end of the box cover is connected to the end of the box body far from the hinge through a lock; multiple groups of limiting seats are arranged, and the multiple groups of limiting seats are evenly installed at the inner bottom end of the box body. Two corresponding limiting seats are symmetrically distributed. The test tube holder is slidably connected to the two symmetrically distributed limiting seats and is detachably connected to the corresponding two limiting seats; and the test tube fixing mechanism described in any one of the above.

[0011] Preferably, two sliding cavities are symmetrically formed on the test tube holder, a handle is slidably connected in the sliding cavity, a first spring is installed on the side wall of the sliding cavity, and the other end of the first spring is connected to the handle; a slot is formed on the limiting seat, and the inserting end of the handle is movably inserted into the slot.

[0012] Compared with the prior art, the above technical solution of the present utility model has the following beneficial technical effects: Through the arrangement of the receiving unit and the abutting unit, test tubes of different sizes containing reagents can be fixedly installed inside the test tube slot, preventing the test tubes from colliding during transportation, having a wider application range, improving the installation stability of the test tubes, and facilitating the quick removal of the test tubes; Through the arrangement of the limiting seats, the handles and the first springs, it is convenient to stably install the test tube holder with multiple groups of test tubes fixedly installed inside the kit, reasonably plan the placement space inside the kit, prevent the test tubes from colliding, prevent the leakage of reagents during transportation, further improve the installation stability of the test tubes, and improve the placement stability of the reagents. Description of the Drawings

[0013] Figure 1 It is a schematic diagram of the installation method of the test tube fixing mechanism of the present utility model and the kit;

[0014] Figure 2 It is a schematic diagram of the connection method between the limiting seat of the present utility model and the kit;

[0015] Figure 3 It is a schematic diagram of the structure of the test tube fixing mechanism of the present utility model;

[0016] Figure 4 It is a schematic diagram of the connection mode between the stabilizing component and the test tube holder of the utility model;

[0017] Figure 5 It is a schematic diagram of the base structure of the utility model.

[0018] Figure numerals: 1, box body; 101, box cover; 2, limit seat; 201, slot; 202, handle; 203, first spring; 3, test tube seat; 301, test tube slot; 302, guide slot; 303, installation cavity; 4, base; 401, groove; 402, slider; 403, second spring; 5, screw; 501, adjusting tube; 502, annular groove; 503, abutment cover; 504, installation hole. DETAILED DESCRIPTION

[0019] Embodiment 1

[0020] like Figures 3 to 5 As shown, a test tube fixing mechanism proposed by the utility model includes a test tube seat 3, a test tube slot 301 and a stabilizing component; two groups of handles 202 are symmetrically arranged on the test tube seat 3; multiple groups of test tube slots 301 are arranged, and the multiple groups of test tube slots 301 are evenly distributed inside the test tube seat 3, and an installation cavity 303 is arranged on one side of the test tube slot 301 and located on the test tube seat 3; the stabilizing component is composed of a receiving unit and an abutting unit, the receiving unit is arranged at the bottom end of the test tube slot 301, and is used to abut with the bottom end of the test tube, and the abutting unit is installed in the installation cavity 303, and is used to detachably connect the top end of the test tube with the receiving unit.

[0021] Furthermore, the receiving unit includes a second spring 403 and a base 4; the second spring 403 is installed at the bottom end of the test tube slot 301; the base 4 is installed at the top of the second spring 403, and the base 4 slides and adjusts along the height direction of the test tube holder 3. A groove 401 is opened at the top of the base 4, and a buffer pad is arranged inside the groove 401.

[0022] Furthermore, multiple groups of sliders 402 are evenly arranged on the side walls of the base 4, and multiple groups of guide grooves 302 are evenly arranged on the side walls of the test tube slot 301 and on the test tube holder 3. The other end of the slider 402 is slidably connected to the corresponding guide groove 302 to prevent the base 4 from tilting inside the test tube slot 301 and jamming test tubes of different diameters, so that the base 4 can always slide and adjust along the height direction of the test tube slot 301.

[0023] Further, the abutting unit includes a screw rod 5, an adjusting tube 501 and an abutting cover 503; the screw rod 5 is installed at the inner bottom end of the installation cavity 303; the adjusting tube 501 is slidably connected to the installation cavity 303 and is threadedly connected to the outer wall of the screw rod 5; one end of the abutting cover 503 is rotatably connected to the upper end of the outer wall of the adjusting tube 501, and a rubber pad is arranged at the bottom of the other end. The rubber pad and the buffer pad on the groove 401 are both provided to reduce the vibration force received by the test tube and prevent the test tube from being clamped by the abutting cover 503 and the base 4, so as to extend the service life; an installation hole 504 is provided at one end of the abutting cover 503 away from the rubber pad, an annular seat is arranged on the inner wall of the installation hole 504, an annular groove 502 is provided on the outer wall of the adjusting tube 501, the abutting cover 503 is sleeved outside the adjusting tube 501 through the installation hole 504, and the annular seat is rotatably connected to the annular groove 502.

[0024] In this embodiment, the test tube with a test tube stopper is inserted along the test tube groove 301 until the bottom end of the test tube contacts the rubber pad on the groove 401. Then, the test tube is pressed down to an appropriate height. At this time, the second spring 403 is compressed. At the same time, the abutting cover 503 is rotated along the adjusting tube 501 until the rubber pad at the bottom end of the abutting cover 503 rotates directly above the test tube stopper. The test tube is released, and under the action of the elastic recovery of the second spring 403, the top end of the test tube is driven to abut against the rubber pad at the bottom end of the abutting cover 503. Thus, the test tube is clamped between the abutting cover 503 and the base 4. To improve the installation stability of the test tube, hold the abutting cover 503 and rotate the adjusting tube 501 so that the abutting cover 503 does not rotate with the adjusting tube 501. When the adjusting tube 501 rotates, it moves downward along the inner wall of the installation cavity 303. At the same time, the adjusting tube 501 moves downward along the screw rod 5 in a threaded manner, further driving the abutting cover 503 to press the test tube tightly against the base 4, thereby clamping the test tube to prevent it from detaching from the test tube groove 301. In this way, multiple groups of test tubes can be fixed in the corresponding test tube grooves 301.

[0025] Embodiment 2

[0026] As Figure 1 、 Figure 2 and Figure 4 shown, a fluorescence quantitative PCR detection kit proposed by the present utility model includes a box body 1, a limiting seat 2 and the test tube fixing mechanism according to any one of claims 1 to 4; one side of the top end of the box body 1 is hingedly connected with a box cover 101, and the other end of the box cover 101 is connected to the end of the box body 1 away from the hinge through a lock; multiple groups of limiting seats 2 are provided, and the multiple groups of limiting seats 2 are evenly installed at the inner bottom end of the box body 1. Two corresponding groups of limiting seats 2 are symmetrically distributed. The test tube seat 3 is slidably connected to the two symmetrically distributed limiting seats 2, and the test tube seat 3 is detachably connected to the corresponding two groups of limiting seats 2.

[0027] Furthermore, two sets of sliding cavities are symmetrically formed in the test tube holder 3, the handle 202 is slidably connected in the sliding cavity, a first spring 203 is installed on the side wall of the sliding cavity, and the other end of the first spring 203 is connected to the handle 202; a slot 201 is formed in the limit seat 2, and the insertion end of the handle 202 is movably inserted into the slot 201, and the handle 202 is L-shaped.

[0028] In this embodiment, the two handles 202 on the test tube holder 3 fixedly installed with multiple groups of test tubes are slid close to each other along the sliding cavity, so that the two first springs 203 are compressed until the insertion ends of the handles 202 are retracted into the sliding cavity. Immediately, the test tube holder 3 is driven by the two handles 202 to slide and insert between the corresponding two limit seats 2 inside the box body 1 until the bottom end of the test tube holder 3 abuts against the bottom end of the box body 1. Release the restriction on the handle 202, and drive the insertion end of the handle 202 to slide along the sliding cavity until it is inserted into the slot 201 under the action of the elastic recovery of the first spring 203, then the test tube holder 3 can be stably installed with the box body 1, and further the multiple groups of test tubes can be stably connected with the box body 1, avoiding shaking during the movement process, improving the installation stability. In this way, the test tube holder 3 fixedly installed with multiple groups of test tubes can be fixedly installed in the box body 1 again. Closing the box body 1 and the box cover 101 can drive the transfer of the reagent in the test tube, avoiding the reagent from spilling due to the collision of the test tubes.

[0029] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited to this. Within the knowledge scope of those skilled in the art to which the present invention pertains, various changes can be made without departing from the purpose of the present invention.

Claims

1. A test tube fixing mechanism, characterized in that, including a test tube holder (3) symmetrically provided with two groups of handles (202) thereon; test tube grooves (301), with multiple groups thereof provided, the multiple groups of test tube grooves (301) being evenly distributed inside the test tube holder (3), and an installation cavity (303) being provided on one side of the test tube groove (301) and on the test tube holder (3); and a stabilizing assembly, which is composed of a receiving unit and an abutting unit, the receiving unit being arranged at the inner bottom end of the test tube groove (301) for abutting against the bottom end of the test tube, and the abutting unit being installed in the installation cavity (303) for detachably connecting the top end of the test tube to the receiving unit.

2. The test tube fixing mechanism according to claim 1, wherein, The receiving unit includes a second spring (403) installed at the inner bottom end of the test tube groove (301); and a base (4) installed at the top end of the second spring (403), the base (4) being slidably adjusted along the height direction of the test tube holder (3), and a groove (401) being provided at the top end of the base (4).

3. The test tube fixing mechanism according to claim 2, characterized in that, Multiple groups of sliders (402) are evenly provided on the side wall of the base (4), and multiple groups of guiding grooves (302) are evenly provided on the side wall of the test tube groove (301) and on the test tube holder (3), and the other end of the slider (402) is slidably connected to the corresponding guiding groove (302).

4. The test tube fixing mechanism according to claim 2, wherein The abutting unit includes a screw rod (5) installed at the inner bottom end of the installation cavity (303); an adjusting tube (501) slidably connected to the installation cavity (303) and threadedly connected to the outer wall of the screw rod (5); and an abutting cover (503) with one end rotatably connected to the upper end of the outer wall of the adjusting tube (501) and a rubber pad provided at the bottom of the other end.

5. A fluorescence quantitative PCR detection kit, characterized in that, including a box body (1) with one side of the top end thereof hingedly connected to a box cover (101), and the other end of the box cover (101) being connected to the end of the box body (1) far from the hinge through a lock; a limiting seat (2), with multiple groups thereof provided, the multiple groups of limiting seats (2) being evenly installed at the inner bottom end of the box body (1), two corresponding groups of limiting seats (2) being symmetrically distributed, the test tube holder (3) being slidably connected to the two symmetrically distributed limiting seats (2), and the test tube holder (3) being detachably connected to the corresponding two groups of limiting seats (2); and the test tube fixing mechanism according to any one of claims 1 to 4.

6. The fluorescence quantitative PCR detection kit according to claim 5, characterized in that, Two groups of sliding cavities are symmetrically provided on the test tube holder (3), the handle (202) is slidably connected in the sliding cavity, a first spring (203) is installed on the side wall of the sliding cavity, and the other end of the first spring (203) is connected to the handle (202); A slot (201) is provided on the limiting seat (2), and the inserting end of the handle (202) is movably inserted into the slot (201).

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