Closed culture system of multi-layer cell culture device

Abstract

The utility model relates to the technical field of cell culture, in particular to a closed culture system of a multi-layer cell culture device. Comprising at least two box bodies which are arranged in parallel, multiple layers of cell culture devices are arranged on the box bodies, first through openings and second through openings are formed in the top faces of the box bodies, the closed culture system of the multiple layers of cell culture devices further comprises parallel hoses and a bandage assembly, the parallel hoses comprise a main pipe and multiple branch pipes connected with the main pipe, and the branch pipes are connected with the main pipe. The branch pipe is connected with the second through opening of each box body; according to the multi-layer cell culture device, a plurality of multi-layer cell culture device box bodies are integrated through the hoses connected in parallel, and then the multi-layer cell culture device box bodies are lifted up through the first binding band and the second binding band to be synchronously lifted and transferred to the automatic operation frame, so that a closed culture system is realized; the culture scale is further enlarged, and the pollution risk caused by manual opening operation is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, and particularly relates to a closed culture system for a multi-layer cell culture device. Background Art

[0002] At present, disposable cell culture devices are popular among users because of their simple process, small hardware investment, and flexible scale change. They are widely used in the production of hepatitis A vaccine, varicella vaccine, and hand-foot-and-mouth disease vaccine. Now, usually 40-layer cell culture devices are placed in a box, and then the box is mechanically placed on an operating rack. Although an advanced automatic operator is used, the transportation of the box still stays at the stage of assembling one by one and opening, and it is impossible to transport them to the operating rack simultaneously for closed culture.

[0003] Therefore, it is necessary to provide a closed culture system for a multi-layer cell culture device that can solve the above problems. Summary of the Utility Model

[0004] In order to solve the problem that multiple cell culture devices cannot be transported to the operating rack simultaneously, the utility model provides a closed culture system for a multi-layer cell culture device, which can transport multiple cell culture devices to the operating rack simultaneously to realize closed culture.

[0005] In order to achieve the above purpose, the utility model provides a closed culture system for a multi-layer cell culture device, which includes at least two boxes arranged side by side. A multi-layer cell culture device is arranged in each box. A first through hole and a second through hole are arranged on the top surface of each box. The closed culture system for the multi-layer cell culture device further includes a parallel hose and a strap assembly. The parallel hose includes a main pipe and a plurality of branch pipes connected to the main pipe. The branch pipes are respectively connected to the first through hole or the second through hole of each box. The strap assembly surrounds the side surface of the box, and a lifting part is arranged at the top of the strap assembly.

[0006] Preferably, the strap assembly includes a first strap and a second strap. The number of the first straps and the second straps is the same as the number of the boxes. The first straps are vertically wound around the upper and lower ends and the side surface of a single box one by one. A lifting part is arranged at the position of the first strap at the top of the box, and the heights of all the lifting parts are the same. The second straps are horizontally wound around the side surface of a single box and the first strap one by one to bind the first strap on the box.

[0007] Preferably, the strap assembly includes a first strap and a second strap. The number of the first straps is the same as that of the boxes. The first straps are vertically wound around the upper and lower ends and the sides of a single box respectively, and a lifting part is arranged at the part of the first strap at the top of the box, and the heights of all the lifting parts are the same; the second strap is horizontally wound around the sides of at least two boxes and the first straps on the boxes, and the second strap is used to bind at least two boxes together and bind the first straps on the boxes.

[0008] Preferably, both the first strap and the second strap are plastic film straps.

[0009] Preferably, the thicknesses of both the first strap and the second strap are 0.05 - 0.30 mm.

[0010] Preferably, the branch pipe and / or the main pipe is a thermoplastic pipe or a silicone pipe.

[0011] Preferably, a detachable air filter is connected to the first through port or the second through port of each box, and / or a detachable air filter is connected to the main pipe; the air filter adopts a sterilizing-grade air filter resistant to gamma-ray irradiation.

[0012] Preferably, the multi-layer cell culture device is packaged and heat-sealed with a double-layer head bag and a plastic bag.

[0013] Preferably, a PET blister box is arranged at the top of the box, and there are several grooves in the blister box, and the grooves are used to place parallel hoses and air filters during transportation.

[0014] Preferably, the first through port and / or the second through port is a small port or a large port. A pagoda hose joint is arranged on the small port, and a conversion cover is arranged on the large port.

[0015] Compared with the prior art, the advantages of the present utility model are as follows: in the closed culture system of the multi-layer cell culture device, a plurality of branch pipes are connected in parallel through the main pipe, so that a plurality of boxes of the multi-layer cell culture device become a whole. Then, the first strap and the second strap are used to lift a plurality of boxes of the multi-layer cell culture device and synchronously lift and transfer them to an automatic operation rack, truly realizing a closed culture system, further expanding the culture scale, reducing the pollution risk of manual opening operation, and having great practical and economic significance for improving large-scale cell preparations. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 is a schematic structural diagram of a closed culture system of a multi-layer cell culture device provided by an embodiment of the present utility model.

[0017] Figure 2It is a schematic structural diagram of the main pipe provided by an embodiment of the present utility model.

[0018] Figure 3 It is a schematic structural diagram of the strap assembly provided by an embodiment of the present utility model.

[0019] Figure 4 It is another schematic structural diagram of the main pipe provided by an embodiment of the present utility model.

[0020] In the figure: 1, box body; 2, air filter; 3, branch pipe; 4, main pipe; 5, first strap; 6, second strap; 7, multi-way joint; 8, lifting part. Specific embodiments

[0021] The accompanying drawings are only for illustrative purposes and cannot be construed as limiting the present utility model; it should be clear that the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments in the present utility model without creative efforts belong to the scope protected by the embodiments of the present utility model.

[0022] The terms used in the embodiments of the present utility model are only for the purpose of describing specific embodiments and are not intended to limit the embodiments of the present utility model. The singular forms "a", "the" and "said" used in the embodiments of the present utility model and the appended claims are also intended to include the plural forms, unless the context clearly indicates otherwise. It should also be understood that the term "and / or" used herein refers to and includes any or all possible combinations of one or more of the associated listed items.

[0023] When the following description relates to the accompanying drawings, unless otherwise indicated, the same numbers in different drawings represent the same or similar elements. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with the present utility model. On the contrary, they are only examples of devices and methods consistent with some aspects of the present utility model as detailed in the appended claims. In the description of the present utility model, it should be understood that the terms "first", "second", "third", etc. are only used to distinguish similar objects and do not have to be used to describe a specific order or sequence, nor can they be construed as indicating or implying relative importance. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0024] In addition, in the description of the present utility model, unless otherwise specified, "a plurality of" means two or more. "And / or" describes the association relationship of associated objects, indicating that there can be three relationships. For example, A and / or B can represent three situations: A exists alone, A and B exist simultaneously, and B exists alone. The character " / " generally represents an "or" relationship between the associated objects before and after. The following further elaborates on the present utility model in conjunction with the accompanying drawings and embodiments.

[0025] The following further elaborates on the present utility model in conjunction with the accompanying drawings and embodiments.

[0026] As Figures 1-4 shown, an embodiment of the present utility model provides a closed culture system for a multi-layer cell culture device. The system includes at least two boxes 1 arranged side by side. A 40-layer cell culture device is provided inside the box 1. Two openings are provided on the top surface of the box 1. Generally, the openings are divided into a first opening and a second opening. A detachable air filter 2 is provided in either the first opening or the second opening. A branch pipe 3 is connected to the second opening or the first opening. The end of the branch pipe 3 is connected to a multi-way joint 7. The multi-way joint 7 is connected to a main pipe 4. A plurality of branch pipes 3 are connected through the main pipe 4; a retention pipe is further provided at one end of the main pipe 4 for retention; the branch pipes 3 of the four boxes 1 are connected in parallel through the multi-way joint 7, making the four 40-layer cell culture devices an integrated whole.

[0027] It should be noted that there is no difference between the first opening and the second opening. The first opening and the second opening can be arbitrarily switched, and their connection relationships are also switched accordingly.

[0028] In a possible embodiment, as Figure 4 shown, a plurality of branch pipes are connected to the main pipe 4 through the multi-way joint 7, and a detachable air filter 2 is also connected to the main pipe 4.

[0029] Furthermore, the branch pipe 3 and the main pipe 4 are made of thermoplastic pipes or silicone pipes, and the air filter 2 is a sterilizing-grade air filter 2 that can withstand gamma-ray irradiation.

[0030] The further improvement lies in that the cell culture device is packaged and heat-sealed with a double-layer head bag and a plastic bag, and then irradiated and sterilized.

[0031] In the illustrated embodiment, a strap assembly is further provided outside the box body 1. The strap assembly includes a first strap 5 and a second strap 6. The number of the first strap 5 and the second strap 6 is the same as that of the box body 1. The first strap 5 is vertically wound around the upper and lower ends and the side surface of the box body 1 one by one. A lifting part 8 is provided at the position of the first strap 5 at the top of the box body 1. The heights of all the lifting parts 8 are the same, so as to facilitate the staff to lift the box body 1 through the lifting part 8 of the first strap 5, so as to achieve the purpose of simultaneously transporting multiple cell culture vessels; the second strap 6 is horizontally wound around the side surface of the box body 1 and the first strap 5, and the second strap 6 can bind the first strap 5 to the box body 1, making the process of lifting the box body 1 more stable and preventing the box body 1 from tipping over.

[0032] In other embodiments, the second strap 6 can also be directly horizontally wound around the side surfaces of two or more box bodies 1 and the corresponding first strap 5, binding several box bodies 1 together and binding the first strap 5 to the box body 1, making the process of lifting the box body 1 more stable. When in use, after opening the packaging bag, a cross bar can be used to pass through the lifting part 8 of the first strap 5, pick up four 40-layer cell culture vessels and lift them synchronously to the automatic operation rack, and then perform the next operation.

[0033] Furthermore, both the first strap 5 and the second strap 6 are plastic film straps. The plastic film can be materials such as PET / PE composite film, nylon film, PE film, PP film, PPO film, etc. Its strength is sufficient to bear the weight of 40 layers of cell culture, and the film thickness is 0.05 - 0.30 mm, so as to avoid the strap being too thick to be placed in the operation trolley.

[0034] In this embodiment, the openings on the box body 1 are divided into two types: small openings and large openings. When the opening is a small opening, a pagoda hose joint is provided on the opening. When the opening is a large opening, a conversion cover is provided on the opening. As Figure 1 shown, the opening in this embodiment is a large opening, and a conversion cover is used to connect to the branch pipe 3 and the air filter 2; generally, the number of openings is two. One opening is connected to the branch pipe 3 through a pagoda hose joint or a conversion cover, and the other opening is connected to the air filter 2 through a pagoda hose joint or a conversion cover.

[0035] In this embodiment, a PET blister box is further provided on the top of the box body 1. There are several grooves in the blister box. The grooves in the PET blister box are used to place the branch pipe 3 and the air filter 2 during transportation.

[0036] In this embodiment, when the multi-layer cell culture device closes the culture system, a stainless steel crossbar can be used to lift four 40-layer cell culture devices and place them on the automatic operation rack, and then a sterile tube connecting machine or liquid operation can be carried out under laminar flow protection. One tube can achieve the operation of 4×40-layer cell culture devices, truly realizing a closed culture system, further expanding the culture scale, reducing the pollution risk of manual opening operation, and having great practical and economic significance for improving large-scale cell preparations.

[0037] The above embodiments are only illustrative of the principles and effects of the present invention, and are not used to limit the present invention. Any person familiar with this technology can modify or change the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or changes completed by those with ordinary knowledge in the technical field without departing from the spirit and technical ideas disclosed by the present invention should still be covered by the claims of the present invention.

Claims

1. A closed culture system for a multi-layer cell culture device, comprising at least two boxes (1) arranged side by side, wherein a multi-layer cell culture device is arranged in each box (1), and a first through port and a second through port are arranged on the top surface of each box (1), and it is characterized in that, The closed culture system of the multi-layer cell incubator further includes a parallel hose and a strap assembly. The parallel hose includes a main pipe (4) and a plurality of branch pipes (3) connected to the main pipe (4). The branch pipes (3) are respectively connected to the first through-port or the second through-port of each box body (1). The strap assembly surrounds the side surface of the box body (1), and a lifting part (8) is provided at the top of the strap assembly.

2. The multi-layer cell culture vessel closed culture system according to claim 1, characterized in that, The strap assembly includes a first strap (5) and a second strap (6). The number of the first straps (5) and the second straps (6) is the same as the number of the box bodies (1). The first straps (5) are vertically wound around the upper and lower ends and the side surface of a single box body (1) one by one. A lifting part (8) is provided at the position of the first strap (5) at the top of the box body (1), and the heights of all the lifting parts (8) are the same. The second straps (6) are horizontally wound around the side surface of a single box body (1) and the first strap (5) one by one, binding the first strap (5) to the box body (1).

3. The multi-layer cell culture vessel closed culture system according to claim 1, characterized in that, The strap assembly includes a first strap (5) and a second strap (6). The number of the first straps (5) is the same as the number of the box bodies (1). The first straps (5) are vertically wound around the upper and lower ends and the side surface of a single box body (1) one by one. A lifting part (8) is provided at the position of the first strap (5) at the top of the box body (1), and the heights of all the lifting parts (8) are the same. The second straps (6) are horizontally wound around the side surfaces of at least two box bodies (1) and the first strap (5) on the box bodies (1), binding at least two box bodies (1) together and binding the first strap (5) to the box body (1).

4. The multi-layer cell culture vessel closed culture system according to any one of claims 2-3, characterized in that, Both the first strap (5) and the second strap (6) are plastic film straps.

5. The multi-layer cell culture vessel closed culture system according to any one of claims 2-3, characterized in that, The thickness of both the first strap (5) and the second strap (6) is 0.05 - 0.30 mm.

6. The closed culture system of the multi-layer cell culture device according to claim 1, characterized in that, The branch pipe (3) and / or the main pipe (4) is a thermoplastic pipe or a silicone tube.

7. The closed culture system of the multi-layer cell culture device according to claim 1, wherein A detachable air filter (2) is connected to the first through-port or the second through-port of each box body (1), or a detachable air filter (2) is connected to the main pipe (4). The air filter (2) is a sterilizing-grade air filter resistant to gamma-ray irradiation.

8. The multi-layer cell culture vessel closed culture system according to claim 7, wherein A PET blister box is provided at the top of the box body (1). There are several grooves in the blister box, and the grooves are used to place the parallel hose and the air filter (2) during transportation.

9. The multi-layer cell culture vessel closed culture system according to claim 1, wherein, The multi-layer cell incubator is packaged and heat-sealed with a double-layer head bag and a plastic bag.

10. The multi-layer cell culture vessel closed culture system according to claim 1, characterized in that, The first through-port and / or the second through-port is a small port or a large port. A pagoda hose joint is provided on the small port, and a conversion cover is provided on the large port.

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