Incubation device for measuring binding rate of medicine and protein

Abstract

The utility model relates to the technical field of incubation devices, in particular to an incubation device for measuring the binding rate of medicine and protein, which comprises an incubator, a control panel and a rotating speed adjustable stack frame, and the rotating speed adjustable stack frame comprises a rotating frame, an incubation plate and a driving motor; the incubator provides installation conditions for the control panel and the stack frames with the adjustable rotating speed, the control panel is used for setting the rotating speed and time of the driving motor, when the stack frames with the adjustable rotating speed are used, the driving motor drives the rotating frame based on the set rotating speed, and the rotating frame rotates to drive the incubation plates to rotate; according to the incubation device, the three driving motors are set to be different in rotating speed and time, three sets of incubation can be carried out at a time, and incubation experiments under different rotating speed and time conditions are met.

Description

Technical Field

[0001] The utility model relates to the technical field of incubation devices, in particular to an incubation device for measuring the binding rate of drugs and proteins. Background Art

[0002] The binding rate of drugs and proteins is one of the important parameters in pharmacokinetics, which affects the distribution, metabolism and excretion of drugs. In the process of clinical and drug research and development, it is of great significance to accurately measure the binding rate of drugs and proteins. The equilibrium dialysis method and the ultra-high-speed centrifugation method are common methods for measuring drug-protein binding rates. The conventional equilibrium dialysis method mostly uses a rapid equilibrium dialysis device (RED) for incubation. The RED system contains disposable tube inserts and is used in combination with a reusable PTFE or a disposable high-density polypropylene 96-well bottom plate. The ultra-high-speed centrifugation method is equipped with ultra-centrifuge tubes of various specifications and a unique rotor, and separation is achieved through an ultra-high-speed centrifuge. Before separating the bound and free parts of drug proteins using the equilibrium dialysis method and the ultra-high-speed centrifugation method, it is necessary to incubate the samples in a carbon dioxide incubator. Traditional incubation devices are difficult to meet the requirements of flexibility and large capacity. Content of the Utility Model

[0003] The purpose of the utility model is to provide an incubation device for measuring the binding rate of drugs and proteins, aiming to solve the problem that traditional incubation devices are difficult to meet the incubation experiments with different centrifugation speeds and time conditions.

[0004] To achieve the above purpose, the utility model provides an incubation device for measuring the binding rate of drugs and proteins, which includes an incubator, a control panel and an adjustable speed stack rack. The control panel is fixedly connected to the incubator and is located outside the incubator. The adjustable speed stack rack is arranged inside the incubator. The adjustable speed stack rack includes a rotating frame, an incubation plate and a driving motor. The rotating frame is rotatably arranged inside the incubator. The output end of the driving motor is fixedly connected to the rotating frame and is fixedly connected to the incubator and is located between the incubator and the rotating frame. The incubation plate is arranged on one side of the rotating frame.

[0005] Among them, the adjustable speed stack rack further includes an ultra-centrifuge tube support, and the ultra-centrifuge tube support is arranged inside the rotating frame.

[0006] Among them, the rotation speed range of the driving motor is set to 200 - 1500 rpm.

[0007] Among them, the incubator includes a box body, a first turntable, a second turntable and a fixing clip. The box body is arranged outside the rotating frame. The first turntable is fixedly connected to the driving motor, rotatably connected to the box body, and located between the driving motor and the box body. The second turntable is fixedly connected to the rotating frame, rotatably connected to the box body, and located between the rotating frame and the box body.

[0008] Among them, the rotating frame includes a placement rack and a sliding rod. The placement rack is fixedly connected to the output end of the driving motor, fixedly connected to the second turntable, and located between the second turntable and the driving motor. The sliding rod is fixedly connected to the placement rack, slidably connected to the first turntable, and located between the placement rack and the first turntable.

[0009] An incubation device for measuring the drug-protein binding rate of the present utility model. The incubator provides installation conditions for the control panel and the adjustable speed stack rack, and is internally provided with a temperature control system and a gas circulation system to provide carbon dioxide and a suitable incubation temperature to maintain a suitable experimental environment. There are three adjustable speed stack racks and several incubation plates. The control panel is used to set the rotation speed and time of the driving motor. The rotation speed range of the driving motor is set to 200 - 1500 rpm, and the time range is set to 0 - 24 hours. The single operation time or continuous operation mode can be accurately set. When the adjustable speed stack rack is in use, the driving motor drives the rotating frame based on the set rotation speed, and the rotating frame rotates to drive the incubation plate to rotate, thereby starting the sample incubation process. It stops rotating until the incubation time reaches the preset time and stops incubation. By setting the three driving motors to different rotation speeds and times without interference, three groups of incubations can be carried out at one time to meet the incubation experiments under different rotation speed and time conditions, solving the problem that traditional incubation devices are difficult to meet the incubation experiments under different rotation speed centrifugation and time conditions. Description of the Drawings

[0010] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art.

[0011] Figure 1 It is the front view of an incubation device for measuring the drug-protein binding rate of the present utility model.

[0012] Figure 2 It is the structural schematic diagram of an incubation device for measuring the drug-protein binding rate of the present utility model.

[0013] Figure 3 It is the structural schematic diagram of the ultracentrifuge tube support of an incubation device for measuring the drug-protein binding rate of the present utility model.

[0014] In the figure: 1 - incubator, 2 - control panel, 3 - adjustable speed stacker, 11 - box body, 12 - first turntable, 13 - second turntable, 14 - fixing clip, 31 - rotating frame, 32 - incubation plate, 33 - driving motor, 34 - ultracentrifugation tube holder, 311 - placement rack, 312 - slide bar. Specific embodiments

[0015] The embodiments of the present invention will be described in detail below. The examples of the embodiments are shown in the drawings, in which the same or similar reference numerals represent the same or similar elements or elements with the same or similar functions throughout. The embodiments described below by referring to the drawings are exemplary and are intended to explain the present invention, but should not be construed as a limitation of the present invention.

[0016] Please refer to Figures 1 to 3 , the present invention provides an incubation device for measuring the drug-protein binding rate, including an incubator 1, a control panel 2 and an adjustable speed stacker 3. The control panel 2 is fixedly connected to the incubator 1 and is located outside the incubator 1. The adjustable speed stacker 3 is arranged in the incubator 1. The adjustable speed stacker 3 includes a rotating frame 31, an incubation plate 32 and a driving motor 33. The rotating frame 31 is rotatably arranged in the incubator 1. The output end of the driving motor 33 is fixedly connected to the rotating frame 31 and is fixedly connected to the incubator 1 and is located between the incubator 1 and the rotating frame 31. The incubation plate 32 is arranged on one side of the rotating frame 31.

[0017] In this embodiment, the incubator 1 provides installation conditions for the control panel 2 and the adjustable speed stacker 3, and is internally provided with a temperature control system and a gas circulation system to provide carbon dioxide and a suitable incubation temperature to maintain a suitable experimental environment. There are three adjustable speed stackers 3, and there are several incubation plates 32. The control panel 2 is used to set the rotation speed and time of the driving motor 33. The rotation speed range of the driving motor 33 is set to 200 - 1500 rpm, and the time range is set to 0 - 24 hours. The single operation time or continuous operation mode can be accurately set. When the adjustable speed stacker 3 is in use, the driving motor 33 drives the rotating frame 31 based on the set rotation speed, and the rotating frame 31 rotates to drive the incubation plate 32 to rotate, thereby starting the sample incubation process. The rotation stops until the incubation time reaches the preset time, and the incubation stops. By setting the three driving motors 33 to different rotation speeds and times, without interference, three groups of incubations can be carried out at one time, meeting the incubation experiments under different rotation speeds and time conditions, and solving the problem that traditional incubation devices are difficult to meet the incubation experiments under different rotation speeds and time conditions.

[0018] Further, the adjustable-speed stacker 3 further includes an ultracentrifugation tube holder 34, and the ultracentrifugation tube holder 34 is disposed inside the rotating frame 31.

[0019] In this embodiment, the ultracentrifugation tube holder 34 is installed inside the rotating frame 31, and can be used interchangeably with the incubation plate 32, or can be used together with the incubation plate 32.

[0020] Further, the incubator 1 includes a box body 11, a first turntable 12, a second turntable 13 and a fixing clip 14. The box body 11 is disposed outside the rotating frame 31. The first turntable 12 is fixedly connected to the driving motor 33 and is rotatably connected to the box body 11, and is located between the driving motor 33 and the box body 11. The second turntable 13 is fixedly connected to the rotating frame 31 and is rotatably connected to the box body 11, and is located between the rotating frame 31 and the box body 11.

[0021] In this embodiment, the box body 11 provides installation conditions for the control panel 2 and the adjustable-speed stacker 3, and is internally provided with a temperature control system and a gas circulation system to provide carbon dioxide and a suitable incubation temperature to maintain a suitable experimental environment. The fixing clip 14 is used to clamp and fix the first turntable 12. The fixing clip 14 is a commonly used technology in the market. During the incubation experiment, the fixing clip 14 clamps and fixes the first turntable 12. The output end of the driving motor 33 drives the rotating frame 31, and the rotation of the rotating frame 31 drives the second turntable 13 to rotate, that is, the sample incubation is realized. When the incubation plate 32 is taken out after incubation, the fixing clip 14 is opened to remove the fixation of the first turntable 12. Since the first turntable 12 and the second turntable 13 are rotatably connected to the box body 11, at this time, the user rotates the first turntable 12 by hand, which can drive the three rotating frames 31 to rotate, and then the incubation plates 32 on each rotating frame 31 can be taken out.

[0022] Further, the rotating frame 31 includes a placement rack 311 and a sliding rod 312. The placement rack 311 is fixedly connected to the output end of the driving motor 33 and is fixedly connected to the second turntable 13, and is located between the second turntable 13 and the driving motor 33. The sliding rod 312 is fixedly connected to the placement rack 311 and is slidably connected to the first turntable 12, and is located between the placement rack 311 and the first turntable 12.

[0023] In this embodiment, the output end of the driving motor 33 rotates to drive the connected placement rack 311, so that the placement rack 311 rotates. The rotation of the placement rack 311 makes the sliding rod 312 slide on the first turntable 12, making the rotation of the placement rack 311 more stable.

[0024] The above-disclosed is only a preferred embodiment of an incubation device for measuring the binding rate of a drug and a protein in this application, and it cannot be used to limit the scope of the rights of this application. Those of ordinary skill in the art can understand all or part of the processes of implementing the above embodiments, and the equivalent changes made according to the claims of this application still fall within the scope covered by this application.

Claims

1. An incubation device for measuring the drug-protein binding rate, characterized in that it includes an incubator, a control panel and an adjustable speed stack rack. The control panel is fixedly connected to the incubator and is located outside the incubator. The adjustable speed stack rack is arranged inside the incubator. The adjustable speed stack rack includes a rotating frame, an incubation plate and a driving motor; the rotating frame is rotatably arranged inside the incubator. The output end of the driving motor is fixedly connected to the rotating frame and is fixedly connected to the incubator and is located between the incubator and the rotating frame. The incubation plate is arranged on one side of the rotating frame.

2. The incubation device for measuring the drug-protein binding rate according to claim 1, characterized in that the adjustable speed stack rack further includes an ultracentrifugation tube holder, and the ultracentrifugation tube holder is arranged inside the rotating frame.

3. The incubation device for measuring the drug-protein binding rate according to claim 1, characterized in that the rotation speed range of the driving motor is set to 200 - 1500 rpm.

4. The incubation device for measuring the drug-protein binding rate according to claim 3, characterized in that the incubator includes a box body, a first turntable, a second turntable and a fixing clip. The box body is arranged outside the rotating frame. The first turntable is fixedly connected to the driving motor and is rotatably connected to the box body and is located between the driving motor and the box body. The second turntable is fixedly connected to the rotating frame and is rotatably connected to the box body and is located between the rotating frame and the box body.

5. The incubation device for measuring the drug-protein binding rate according to claim 4, characterized in that the rotating frame includes a placement rack and a sliding rod. The placement rack is fixedly connected to the output end of the driving motor and is fixedly connected to the second turntable and is located between the second turntable and the driving motor. The sliding rod is fixedly connected to the placement rack and is slidably connected to the first turntable and is located between the placement rack and the first turntable.

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