WB antibody incubation box capable of being stacked

By setting up sealing and closing mechanisms in the WB antibody incubation box, the cross-contamination problem caused by the splash of antibody liquid is solved, and the stability of the antibody solution and the cleanliness of the incubation environment are achieved.

CN223155024UActive Publication Date: 2025-07-25SHENZHEN KANGZHIRUI BIOTECHNOLOGY INNOVATION SERVICE CO LTD
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Patent Information

Application Number
CN202422262995.0
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-09-14
Publication Date
2025-07-25
Estimated Expiration
2034-09-14

AI Technical Summary

Technical Problem

When the existing incubation box is placed or moved, the antibody liquid is prone to splash due to shaking or tilting, resulting in cross-contamination between different incubation boxes, affecting the reliability of the experimental results.

Method used

A stackable WB antibody incubation box is designed, and a sealing mechanism and a closure mechanism are provided, including a polyethylene sealing film and elastic components, which form a closed space through the sealing mechanism to avoid splashing of antibody liquid; the closure mechanism ensures that the box door is firmly closed and prevents external contaminants from entering.

Benefits of technology

Effectively prevent the splash and diffusion of antibody liquid, avoid cross-contamination, keep the concentration of antibody solution stable, prevent dust and bacterial contamination, and ensure the cleanliness of the incubation environment.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model discloses a stackable WB antibody incubation box, and relates to the technical field of antibody incubation. The device comprises an incubation box, wherein a plurality of sealing mechanisms and closing mechanisms are arranged on the incubation box; a box door is hinged to the front side of the incubation box, a plurality of trapezoidal sliding grooves are formed in the inner walls of the left side and the right side of the incubation box, a plurality of incubation plates are slidably connected to the inner walls of the trapezoidal sliding grooves, incubation grooves are formed in the tops of the incubation plates, and the sealing mechanism comprises a sealing assembly and an elastic assembly. Through the arrangement of the sealing mechanism, the problems that when existing incubation boxes are placed or moved in a laminated mode, antibody liquid in the boxes is splashed due to shaking, inclining and the like due to no blocking of a sealing film, cross contamination is generated when samples in different incubation boxes make contact with the splashed antibody liquid, and the incubation time is shortened are solved. And the reliability of the experiment result is lost.
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Description

Technical Field

[0001] The utility model belongs to the technical field of antibody incubation, and particularly relates to a stackable WB antibody incubation box. Background Art

[0002] WB is an important and common basic experimental technique in biomedical research, which is used to analyze the expression of specific proteins in cells or tissues. This experiment includes steps such as gel preparation, sample loading, electrophoresis, membrane transfer, blocking, primary antibody incubation, secondary antibody incubation, and exposure. Antibody incubation is one of the most important steps, which makes the antibody incubation box an essential consumable in this experimental process. With the continuous deepening of life science research, the demand for WB experiments is increasing day by day, so the demand for antibody incubation boxes is also constantly rising.

[0003] However, when the existing incubation boxes are stacked or moved, without the barrier of the sealing film, the antibody solution in the box will splash due to reasons such as shaking and tilting. If the samples in different incubation boxes come into contact with the splashed antibody solution, cross - contamination will occur, making the experimental results unreliable. Summary of the Utility Model

[0004] The purpose of the utility model is to provide a stackable WB antibody incubation box. By setting a sealing mechanism, it solves the problem that when the existing incubation boxes are stacked or moved, without the barrier of the sealing film, the antibody solution in the box will splash due to reasons such as shaking and tilting. If the samples in different incubation boxes come into contact with the splashed antibody solution, cross - contamination will occur, making the experimental results unreliable.

[0005] To solve the above - mentioned technical problems, the utility model is realized through the following technical solutions:

[0006] The utility model is a stackable WB antibody incubation box, which includes an incubation box. A number of sealing mechanisms and closing mechanisms are arranged on the incubation box;

[0007] A box door is hinged on the front side of the incubation box. A number of trapezoidal chutes are opened on the inner walls of the left and right sides of the incubation box. A number of incubation plates are slidably connected to the inner walls of the trapezoidal chutes. Incubation grooves are opened on the tops of the incubation plates. The sealing mechanism includes a sealing component and an elastic component. The sealing component includes a polyethylene sealing film arranged above the incubation plate, and a number of support rods are fixedly connected to the top of the incubation plate.

[0008] Furthermore, two support plates are fixedly connected to the tops of the support rods, and rectangular chutes are opened between the support rods.

[0009] Further, two pressing plates are slidably connected to the inner walls of several of the rectangular chutes. Gaskets are fixedly connected to the bottoms of the two pressing plates, and the bottoms of the two gaskets are in contact with the polyethylene sealing film.

[0010] Further, the elastic component includes two telescopic rods respectively fixedly connected to the tops of the two pressing plates. The tops of several of the telescopic rods are respectively fixedly connected to the two support plates. Springs I are sleeved on the outer walls of several of the telescopic rods. The tops of several of the Springs I are respectively fixedly connected to the two support plates, and the bottoms of several of the Springs I are respectively fixedly connected to the two pressing plates.

[0011] Further, sliding rods I are fixedly connected to the tops of the two pressing plates. The tops of the two sliding rods I respectively slide through the outer walls of the two support plates and extend to the outside. Handles I are fixedly connected to the tops of the two sliding rods I.

[0012] Further, the closing mechanism includes a first closing component and a second closing component. The first closing component includes an L-shaped plate fixedly connected to the left side of the box door. A sleeve is fixedly connected to the left side of the L-shaped plate.

[0013] Further, a circular slider is slidably connected to the inner wall of the sleeve. A limiting rod is fixedly connected to the right side of the circular slider. The right side of the limiting rod slides and extends into the incubation box.

[0014] Further, the second closing component includes a sliding rod II fixedly connected to the left side of the circular slider. The left side of the sliding rod II slides and extends outside the sleeve. A spring II is sleeved on the outer wall of the sliding rod II. The left side of the spring II is fixedly connected to the sleeve, and the right side of the spring II is fixedly connected to the circular slider. A handle II is fixedly connected to the extending part on the left side of the sliding rod II.

[0015] The utility model has the following beneficial effects:

[0016] 1. By providing a sealing mechanism, after the processed imprinting film is placed, the handle I can be pulled. The handle I drives the pressing plate to move upward through the sliding rod I, thereby forcing the telescopic rod and the spring I to be in a compressed state. The prepared polyethylene sealing film is laid flat on the incubation plate to cover the incubation groove. At this time, the handle I is released, and the pressing plate will move downward under the action of the elastic force of the spring I, thereby stably fixing the polyethylene sealing film on the incubation plate, so that a relatively closed space can be formed, avoiding the splashing or diffusion of the antibody solution between different samples and causing cross-contamination, and also preventing the volatilization of the antibody solution and maintaining the stable concentration of the antibody solution;

[0017] 2. By setting up a closing mechanism, after being fixed, the incubation plate can be placed in the incubation box through the trapezoidal chute. Pull the second handle, and the second handle drives the limiting rod on the circular slider to move leftward through the second sliding rod. At this time, the second spring is in a compressed state. Close the box door. After the box door is completely closed, release the second handle. At this time, the limiting rod on the circular slider will be reset under the elastic force of the second spring and inserted into the incubation box to complete the fixation of the box door, enabling the box door to be firmly fixed, preventing the box door from accidentally opening, thereby effectively preventing pollutants such as dust and bacteria from entering the incubation box and maintaining the cleanliness of the incubation environment.

[0018] Of course, it is not necessary for any product implementing the present utility model to achieve all the above-mentioned advantages simultaneously. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the following will briefly introduce the drawings required for describing the embodiments. Obviously, the drawings in the following description are only some embodiments of the present utility model. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0020] Figure 1 is a schematic diagram of the overall structure of the present utility model;

[0021] Figure 2 is a schematic diagram of the top cross-sectional structure of the present utility model;

[0022] Figure 3 is a schematic diagram of the sealing mechanism structure of the present utility model;

[0023] Figure 4 For the present utility model Figure 2 is an enlarged schematic diagram of A in the present utility model;

[0024] Figure 5 For the present utility model Figure 3 is an enlarged schematic diagram of B in the present utility model.

[0025] In the drawings, the list of components represented by each reference numeral is as follows:

[0026] 1. Incubation box; 101. Box door; 103. Trapezoidal chute; 104. Incubation plate; 105. Incubation groove; 2. Sealing mechanism; 21. Sealing assembly; 211. Polyethylene sealing film; 212. Support rod; 213. Support plate; 214. Rectangular chute; 215. Pressing plate; 216. Gasket; 22. Elastic assembly; 221. Telescopic rod; 222. First spring; 223. First sliding rod; 224. First handle; 3. Closing mechanism; 31. First closing assembly; 311. L-shaped plate; 312. Sleeve; 313. Circular slider; 314. Limit rod; 32. Second closing assembly; 321. Second sliding rod; 322. Second spring; 323. Second handle. Detailed implementation mode

[0027] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0028] Please refer to Figures 1-5As shown in the figure, the utility model relates to a stackable WB antibody incubation box, which includes an incubation box 1. A number of sealing mechanisms 2 and closing mechanisms 3 are arranged on the incubation box 1. A box door 101 is hinged on the front side of the incubation box 1. A number of trapezoidal chutes 103 are opened on the inner walls of the left and right sides of the incubation box 1. A number of incubation plates 104 are slidably connected to the inner walls of the number of trapezoidal chutes 103. Incubation grooves 105 are opened on the tops of the number of incubation plates 104. The sealing mechanism 2 includes a sealing component 21 and an elastic component 22. The sealing component 21 includes a polyethylene sealing film 211 arranged above the incubation plate 104. A number of support rods 212 are fixedly connected to the top of the incubation plate 104. Two support plates 213 are fixedly connected to the tops of the number of support rods 212. Rectangular chutes 214 are opened between the number of support rods 212. Two pressing plates 215 are slidably connected to the inner walls of the number of rectangular chutes 214. Gaskets 216 are fixedly connected to the bottoms of the two pressing plates 215. The bottoms of the two gaskets 216 are in contact with the polyethylene sealing film 211. The elastic component 22 includes two telescopic rods 221 respectively fixedly connected to the tops of the two pressing plates 215. The tops of the number of telescopic rods 221 are respectively fixedly connected to the two support plates 213. A first spring 222 is sleeved on the outer walls of the number of telescopic rods 221. The tops of the number of first springs 222 are respectively fixedly connected to the two support plates 213. The bottoms of the number of first springs 222 are respectively fixedly connected to the two pressing plates 215. Slide rods 223 are fixedly connected to the tops of the two pressing plates 215. The tops of the two slide rods 223 respectively slide through the outer walls of the two support plates 213 and extend to the outside. Handles 224 are fixedly connected to the tops of the two slide rods 223. By setting the sealing mechanism 2, a relatively enclosed space can be formed, avoiding the splashing or diffusion of the antibody solution between different samples and causing cross-contamination, and also preventing the volatilization of the antibody solution and maintaining the stable concentration of the antibody solution.

[0029] The closing mechanism 3 includes a first closing component 31 and a second closing component 32. The first closing component 31 includes an L-shaped plate 311 fixedly connected to the left side of the cabinet door 101. A sleeve 312 is fixedly connected to the left side of the L-shaped plate 311. A circular slider 313 is slidably connected to the inner wall of the sleeve 312. A limiting rod 314 is fixedly connected to the right side of the circular slider 313. The right side of the limiting rod 314 slidably extends into the incubation box 1. The second closing component 32 includes a second sliding rod 321 fixedly connected to the left side of the circular slider 313. The left side of the second sliding rod 321 slidably extends outside the sleeve 312. A second spring 322 is sleeved on the outer wall of the second sliding rod 321. The left side of the second spring 322 is fixedly connected to the sleeve 312. The right side of the second spring 322 is fixedly connected to the circular slider 313. A second handle 323 is fixedly connected to the extended portion on the left side of the second sliding rod 321. By providing the closing mechanism 3, the cabinet door can be firmly fixed to prevent the cabinet door from accidentally opening, thereby effectively preventing pollutants such as dust and bacteria from entering the incubation box and maintaining the cleanliness of the incubation environment.

[0030] A specific application of this embodiment is as follows: When in use, first place the device in the corresponding position, take out the incubation plate 104, carefully place the processed blotting membrane into the incubation groove 105, ensure that the membrane is flat and completely immersed in the antibody solution. After placement, pull the first handle 224. The first handle 224 drives the pressing plate 215 to move upward through the first sliding rod 223, thereby forcing the telescopic rod 221 and the first spring 222 to be in a compressed state. Lay the prepared polyethylene sealing film 211 flat on the incubation plate 104 so that it covers the incubation groove 105. At this time, release the first handle 224, and the pressing plate 215 will move downward under the action of the elastic force of the first spring 222, thereby stably fixing the polyethylene sealing film 211 on the incubation plate 104, enabling a relatively closed space to be formed, avoiding the splashing or diffusion of the antibody solution between different samples and causing cross-contamination, and also preventing the volatilization of the antibody solution and maintaining the stability of the concentration of the antibody solution. After fixation, the incubation plate 104 can be placed in the incubation box 1 through the trapezoidal sliding groove 103. Pull the second handle 323. The second handle 323 drives the limiting rod 314 on the circular slider 313 to move leftward through the second sliding rod 321. At this time, the second spring 322 is in a compressed state. Close the cabinet door 101. After the cabinet door 101 is completely closed, release the second handle 323. At this time, the limiting rod 314 on the circular slider 313 will be reset under the action of the elastic force of the second spring 322 and insert into the incubation box 1 to complete the fixation of the cabinet door 101, enabling the cabinet door to be firmly fixed to prevent the cabinet door from accidentally opening, thereby effectively preventing pollutants such as dust and bacteria from entering the incubation box and maintaining the cleanliness of the incubation environment.

[0031] In the description of this specification, the descriptions referring to terms such as "one embodiment", "example", "specific example", etc. mean that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present utility model. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0032] The preferred embodiments of the present utility model disclosed above are only used to help illustrate the present utility model. The preferred embodiments do not describe all the details in detail, nor do they limit the present utility model to the specific embodiments described. Obviously, many modifications and variations can be made according to the content of this specification. These embodiments are selected and specifically described in this specification in order to better explain the principle and practical application of the present utility model, so that those skilled in the relevant technical field can well understand and utilize the present utility model. The present utility model is only limited by the claims and their full scope and equivalents.

Claims

1. A stackable WB antibody incubation box, comprising an incubation box (1), wherein a plurality of sealing mechanisms (2) and closing mechanisms (3) are arranged on the incubation box (1). It is characterized in that; A box door (101) is hinged to the front side of the incubation box (1). A plurality of trapezoidal chutes (103) are provided on the inner walls of the left and right sides of the incubation box (1). A plurality of incubation plates (104) are slidably connected to the inner walls of the plurality of trapezoidal chutes (103). Incubation grooves (105) are provided on the tops of the plurality of incubation plates (104). The sealing mechanism (2) includes a sealing component (21) and an elastic component (22). The sealing component (21) includes a polyethylene sealing film (211) arranged above the incubation plate (104). A plurality of support rods (212) are fixedly connected to the top of the incubation plate (104).

2. The stackable WB antibody incubation box according to claim 1, wherein, Two support plates (213) are fixedly connected to the tops of the plurality of support rods (212). Rectangular chutes (214) are provided between the plurality of support rods (212).

3. The stackable WB antibody incubation box according to claim 2, characterized in that, Two pressing plates (215) are slidably connected to the inner walls of the plurality of rectangular chutes (214). Gaskets (216) are fixedly connected to the bottoms of the two pressing plates (215). The bottoms of the two gaskets (216) are in contact with the polyethylene sealing film (211).

4. The stackable WB antibody incubation box according to claim 3, characterized in that, The elastic component (22) includes two telescopic rods (221) respectively fixedly connected to the tops of the two pressing plates (215). The tops of the plurality of telescopic rods (221) are respectively fixedly connected to the two support plates (213). Spring I (222) is sleeved on the outer walls of the plurality of telescopic rods (221). The tops of the plurality of spring I (222) are respectively fixedly connected to the two support plates (213). The bottoms of the plurality of spring I (222) are respectively fixedly connected to the two pressing plates (215).

5. The stackable WB antibody incubation box according to claim 4, wherein Two sliding rods I (223) are fixedly connected to the tops of the two pressing plates (215). The tops of the two sliding rods I (223) respectively slide through the outer walls of the two support plates (213) and extend to the outside. Handles I (224) are fixedly connected to the tops of the two sliding rods I (223).

6. The stackable WB antibody incubation box according to claim 5, wherein, The closing mechanism (3) includes a closing component I (31) and a closing component II (32). The closing component I (31) includes an L-shaped plate (311) fixedly connected to the left side of the box door (101). A sleeve (312) is fixedly connected to the left side of the L-shaped plate (311).

7. A stackable WB antibody incubation box according to claim 6, wherein, A circular slider (313) is slidably connected to the inner wall of the sleeve (312). A limiting rod (314) is fixedly connected to the right side of the circular slider (313). The right side of the limiting rod (314) slides and extends into the incubation box (1).

8. A stackable WB antibody incubation box according to claim 7, characterized in that, The closing component two (32) includes a second slide bar (321) fixedly connected to the left side of the circular slider (313). The left side of the second slide bar (321) slidably extends outside the sleeve (312). A second spring (322) is sleeved on the outer wall of the second slide bar (321). The left side of the second spring (322) is fixedly connected to the sleeve (312), and the right side of the second spring (322) is fixedly connected to the circular slider (313). A second handle (323) is fixedly connected to the extended part on the left side of the second slide bar (321).