Microfluidic immunoassay detection chip

Abstract

The utility model relates to the technical field of microfluidics, in particular to a microfluidic immunoassay detection chip which comprises a chip main body, a sample introduction channel on the chip main body, a sample cavity arranged at one end of the sample introduction channel, a marker cavity arranged at one side of the sample cavity, a first pump port connected with the sample cavity and the marker cavity, and a second pump port connected with the marker cavity. The sample injection channel is connected with the mixing channel, a first fixing plate is arranged in the sample injection channel, a fixing cylinder is arranged on one side face of the first fixing plate, a first pressure spring is arranged in the fixing cylinder, and an extrusion plate is arranged at one end of the first pressure spring. The problems that the temperature in the reaction cavity needs to be kept constant and an anti-backflow structure can be reinforced are solved.

Description

Technical Field

[0001] The utility model relates to the technical field of microfluidics, and particularly relates to a microfluidic immunoassay detection chip. Background Technique

[0002] Microfluidic chip technology (Microfluidics) integrates basic operation units such as sample reaction and detection in the biological, chemical, and medical analysis processes onto a chip at the micron scale to automatically complete the entire analysis process. The characteristics and development advantages of microfluidic chips are as follows: microfluidic chips have controllable liquid flow, consume extremely small amounts of samples and reagents (microfluidic chips can transport fluids from 10-6L to 10-15L), produce less waste liquid from analysis, and the analysis speed is increased by ten times or hundreds of times. It can perform high-throughput analysis of hundreds of samples in a few minutes or even less time.

[0003] Moreover, in the application document with the publication number CN219334244U, a microfluidic immunoassay detection chip is mentioned. This ceramic handicraft includes a sample chamber, a marker chamber, a sample injection channel, a cleaning channel, a reaction detection chamber, and a waste liquid chamber through the microfluidic immunoassay detection chip; the sample chamber is provided with a sample injection hole for adding samples, the marker chamber is pre-filled with quantum dot microsphere markers, both the sample chamber and the marker chamber are connected to a pump port assembly for pumping in diluent, and both the sample chamber and the marker chamber are connected to one end of the sample injection channel; one end of the cleaning channel is provided with a third pump port for pumping in cleaning liquid; the reaction detection chamber is connected to the other end of the sample injection channel and the other end of the cleaning channel, and a capture microarray is arranged in the reaction detection chamber; the waste liquid chamber is connected to the reaction detection chamber to collect waste liquid. In the above microfluidic immunoassay detection chip, because the marker chamber is pre-filled with quantum dot microsphere markers and a capture microarray is arranged in the reaction detection chamber, the sample can be mixed with the quantum dot microsphere markers and incubated on the capture microarray, or the sample can be incubated on the capture microarray, washed, and then mixed with the quantum dot microsphere markers. Then, using a supporting detection instrument, fluorescence detection is performed on the capture microarray, so as to combine the quantum dot fluorescence microsphere technology and the protein microarray technology to realize quantitative, semi-quantitative, or qualitative analysis of immune disease-related molecules, and the detection time and process are greatly shortened. Thus, it solves the problem that existing immunoassay devices cannot effectively balance detection efficiency and detection accuracy, but there are still certain defects that need to be optimized. The specific defects are as follows: a constant temperature needs to be maintained in the reaction chamber, and the anti-backflow structure can be strengthened.

[0004] Therefore, it is particularly important to design a microfluidic immunoassay detection chip to change the above technical defects and improve the overall practicality. Content of the Utility Model

[0005] The purpose of the present utility model is to provide a microfluidic immunoassay detection chip to solve the problems raised in the above-mentioned background technology.

[0006] To achieve the above object, the present utility model provides the following technical solutions:

[0007] A microfluidic immunoassay detection chip includes a chip body. There is a sample injection channel on the chip body. One end of the sample injection channel is provided with a sample chamber. A marker chamber is provided on one side of the sample chamber. Both the sample chamber and the marker chamber are connected to a first pump port. The sample injection channel is connected to a mixing channel. A first fixing plate is provided inside the sample injection channel. A fixing cylinder is provided on one side surface of the first fixing plate. A first compression spring is provided inside the fixing cylinder. One end of the first compression spring is provided with a pressing plate.

[0008] The mixing channel is connected to a reaction chamber. The mixing channel is connected to a cleaning liquid channel. One end of the cleaning liquid channel is connected to a second pump port. A second fixing plate is provided inside the cleaning liquid channel. A second compression spring is provided on one side surface of the second fixing plate. One end of the second compression spring is provided with a limiting plate. The reaction chamber is connected to a waste liquid chamber. A transparent cover plate is provided on one side surface of the chip body. A temperature sensor is provided on one side of the reaction chamber.

[0009] As a preferred scheme of the present utility model, avoiding grooves are provided on the transparent cover plate at positions corresponding to the first pump port, the sample chamber, the marker chamber, and the second pump port. And cover cylinders are provided on the avoiding grooves corresponding to the sample chamber and the marker chamber. The cover cylinders and the avoiding grooves are in snap connection.

[0010] As a preferred scheme of the present utility model, the sample injection channel is in a Y shape, and the sample chamber and the marker chamber are arranged in parallel. The mixing channel is in a serpentine shape.

[0011] As a preferred scheme of the present utility model, a number of avoiding holes are provided on the first fixing plate and the second fixing plate. The a number of avoiding holes are arranged at equal intervals.

[0012] As a preferred scheme of the present utility model, a thermoelectric cooler is provided on the transparent cover plate at a position corresponding to the reaction chamber. Ventilation holes are provided on the transparent cover plate.

[0013] As a preferred scheme of the present utility model, the pressing plate and the limiting plate are in an arc shape. And the pressing plate and the limiting plate are fixedly connected to one ends of the first compression spring and the second compression spring respectively.

[0014] Compared with the prior art, the beneficial effects of the present utility model are:

[0015] In the present utility model, a microfluidic immunoassay detection chip is provided. By using the structure of a fixing plate, a fixing cylinder, a compression spring, an extrusion plate, and a limiting plate, the position of the limiting plate and the extrusion plate is driven by the compression spring provided, so that the solution cannot flow back. At the same time, when the solution flows normally, the compression spring extends, and the limiting plate and the extrusion plate do not prevent the solution from flowing. At the same time, a temperature sensor is provided on one side of the reaction chamber, and the thermoelectric cooler is started according to the value of the temperature sensor to keep the temperature sensor at a constant temperature, solving the problem that a constant temperature needs to be maintained in the reaction chamber and the anti-backflow structure can be strengthened. Brief Description of the Drawings

[0016] Figure 1 It is a structural diagram of the overall disassembly of the present utility model;

[0017] Figure 2 It is a schematic diagram of the overall present utility model;

[0018] Figure 3 It is a schematic diagram of the extrusion plate and limiting plate assembly of the present utility model.

[0019] In the figure: 1. Chip body; 101. Sampling channel; 102. Sample chamber; 103. Marker chamber; 104. First pump port; 105. Mixing channel; 106. Reaction chamber; 107. Transparent cover plate; 108. Temperature sensor; 2. First fixing plate; 201. Fixing cylinder; 202. First compression spring; 203. Extrusion plate; 3. Cleaning liquid channel; 302. Second pump port; 303. Second fixing plate; 304. Second compression spring; 305. Limiting plate; 306. Waste liquid chamber. Detailed Embodiments

[0020] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0021] To facilitate the understanding of the present utility model, the present utility model will be described more comprehensively with reference to the relevant drawings. Several embodiments of the present utility model are given. However, the present utility model can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present utility model more thorough and comprehensive.

[0022] It should be noted that when an element is referred to as being "fixed to" another element, it can be directly on the other element or there may also be an intermediate element. When an element is considered to be "connected to" another element, it can be directly connected to the other element or there may be an intermediate element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only.

[0023] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field to which this utility model belongs. The terms used herein in the description of this utility model are only for the purpose of describing specific embodiments and are not intended to limit this utility model. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0024] For the embodiments, please refer to Figures 1-3 , this utility model provides a technical solution:

[0025] A microfluidic immunoassay detection chip, comprising a chip body 1, a sample injection channel 101 on the chip body 1, a sample chamber 102 provided at one end of the sample injection channel 101, a marker chamber 103 provided on one side of the sample chamber 102, both the sample chamber 102 and the marker chamber 103 are connected to a first pump port 104, the sample injection channel 101 is connected to a mixing channel 105, a first fixing plate 2 is provided inside the sample injection channel 101, a fixing cylinder 201 is provided on one side surface of the first fixing plate 2, a first compression spring 202 is provided inside the fixing cylinder 201, an extrusion plate 203 is provided at one end of the first compression spring 202, the mixing channel 105 is connected to a reaction chamber 106, the mixing channel 105 is connected to a cleaning liquid channel 3, one end of the cleaning liquid channel 3 is connected to a second pump port 302, a second fixing plate 303 is provided inside the cleaning liquid channel 3, a second compression spring 304 is provided on one side surface of the second fixing plate 303, a limiting plate 305 is provided at one end of the second compression spring 304, the reaction chamber 106 is connected to a waste liquid chamber 306, a transparent cover plate 107 is provided on one side surface of the chip body 1, a temperature sensor 108 is provided on one side of the reaction chamber 106, a test sample and a marker solution are respectively added into the sample chamber 102 and the marker chamber 103 through the first pump port 104, the sample and the marker solution are pushed by the pump force, pass through the Y-shaped sample injection channel 101, and enter the serpentine mixing channel 105 for sufficient mixing. Among them, the solution passes through the avoidance hole on the first fixing plate 2, pushes the extrusion plate 203 to make the first compression spring 202 stretch, so that the mixed solution flows into the reaction chamber 106, and meets the cleaning liquid introduced through the cleaning liquid channel 3 here. The limiting plate 305 in the cleaning liquid channel 3 is the same as the extrusion plate 203 to prevent the cleaning liquid from flowing back. A temperature sensor 108 is provided on one side of the reaction chamber 106 for real-time temperature monitoring, and precise temperature control is carried out through the thermoelectric cooler on the transparent cover plate 107 to keep the inside of the reaction chamber 106 at a constant temperature. After the reaction is completed, the waste liquid is discharged through the waste liquid chamber 306;

[0026] An avoidance groove is provided on the transparent cover plate 107 at positions corresponding to the first pump port 104, the sample chamber 102, the marker chamber 103, and the second pump port 302. A cover cylinder is provided on the avoidance groove corresponding to the sample chamber 102 and the marker chamber 103. The cover cylinder and the avoidance groove are in a snap-fit connection, which is convenient for adding solution into the sample chamber 102 and the marker chamber 103 and then sealing to keep it clean. The sampling channel 101 is in a Y shape, and the sample chamber 102 and the marker chamber 103 are arranged in parallel. The mixing channel 105 is in a serpentine shape, which is convenient for sufficient mixing between the sample solution and the marker solution. A number of avoidance holes are provided on the first fixing plate 2 and the second fixing plate 303, and the number of avoidance holes are arranged at equal intervals, which is convenient for the mixed solution to flow downward through the channel into the next reaction chamber. A thermoelectric cooler is provided at the position of the transparent cover plate 107 corresponding to the reaction chamber 106. The transparent cover plate 107 is provided with air holes, which is convenient for keeping the temperature inside the reaction chamber constant. The pressing plate 203 and the limiting plate 305 are in an arc shape, and the pressing plate 203 and the limiting plate 305 are fixedly connected to one end of the first compression spring 202 and the second compression spring 304 respectively, which is convenient for controlling the positions of the pressing plate 203 and the limiting plate 305 by the contraction of the compression spring to prevent the solution from flowing back.

[0027] The working process of the present utility model: When using this kind of microfluidic immunoassay detection chip, first, a sample to be tested and a marker solution are respectively added into the sample chamber 102 and the marker chamber 103 through the first pump port 104. Under the push of the pump force, the sample and the marker solution pass through the Y-shaped sampling channel 101 and enter the serpentine mixing channel 105 for sufficient mixing. Among them, the solution passes through the avoidance holes on the first fixing plate 2, pushes the pressing plate 203 to make the first compression spring 202 stretch, so that the mixed solution flows into the reaction chamber 106, and meets the cleaning solution introduced through the cleaning solution channel 3 here. The limiting plate 305 in the cleaning solution channel 3 is the same as the pressing plate 203 to prevent the cleaning solution from flowing back. A temperature sensor 108 is provided on one side of the reaction chamber 106 for real-time temperature monitoring, and precise temperature control is carried out through the thermoelectric cooler on the transparent cover plate 107 to keep the temperature inside the reaction chamber 106 constant. After the reaction is completed, the waste liquid is discharged through the waste liquid chamber 306. Among them, quantum dot microsphere markers are pre-loaded in the marker chamber 103, and a capture microarray is arranged in the reaction chamber 106. The sample can be incubated with the quantum dot microsphere markers on the capture microarray after mixing, or the sample can be incubated on the capture microarray, washed and then mixed with the quantum dot microsphere markers. Then, a supporting detection instrument is used to perform fluorescence detection on the capture microarray, so as to combine the quantum dot fluorescence microsphere technology and the protein microarray technology to realize quantitative, semi-quantitative or qualitative analysis of immune disease-related molecules. This is an existing mature technology, so it will not be elaborated here.

[0028] Although embodiments of the present utility model have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principle and spirit of the present utility model. The scope of the present utility model is defined by the appended claims and their equivalents.

Claims

1. A microfluidic immunoassay detection chip, comprising a chip body (1), characterized in that: On the chip body (1), there is a sample injection channel (101). One end of the sample injection channel (101) is provided with a sample chamber (102). On one side of the sample chamber (102), there is a marker chamber (103). Both the sample chamber (102) and the marker chamber (103) are connected to the first pump port (104). The sample injection channel (101) is connected to a mixing channel (105). Inside the sample injection channel (101), there is a first fixing plate (2). On one side surface of the first fixing plate (2), there is a fixing cylinder (201). Inside the fixing cylinder (201), there is a first compression spring (202). One end of the first compression spring (202) is provided with a pressing plate (203). The mixing channel (105) is connected to a reaction chamber (106). The mixing channel (105) is connected to a cleaning liquid channel (3). One end of the cleaning liquid channel (3) is connected to the second pump port (302). Inside the cleaning liquid channel (3), there is a second fixing plate (303). On one side surface of the second fixing plate (303), there is a second compression spring (304). One end of the second compression spring (304) is provided with a limiting plate (305). The reaction chamber (106) is connected to a waste liquid chamber (306). On one side surface of the chip body (1), there is a transparent cover plate (107). On one side of the reaction chamber (106), there is a temperature sensor (108).

2. The microfluidic immunoassay detection chip according to claim 1, characterized in that: On the transparent cover plate (107), avoidance grooves are provided at positions corresponding to the first pump port (104), the sample chamber (102), the marker chamber (103), and the second pump port (302). And on the avoidance grooves corresponding to the sample chamber (102) and the marker chamber (103), there are cover cylinders. The cover cylinders and the avoidance grooves are in snap connection.

3. The microfluidic immunoassay detection chip according to claim 1, characterized in that: The sample injection channel (101) is in a Y shape, and the sample chamber (102) and the marker chamber (103) are arranged in parallel. The mixing channel (105) is in a serpentine shape.

4. The microfluidic immunoassay detection chip according to claim 1, wherein: The first fixing plate (2) and the second fixing plate (303) are provided with a number of avoidance holes, and the number of avoidance holes are arranged at equal intervals.

5. A microfluidic immunoassay detection chip according to claim 1, characterized in that: At a position of the transparent cover plate (107) corresponding to the reaction chamber (106), there is a thermoelectric cooler, and the transparent cover plate (107) is provided with ventilation holes.

6. The microfluidic immunoassay detection chip according to claim 1, wherein: The pressing plate (203) and the limiting plate (305) are in an arc shape, and the pressing plate (203) and the limiting plate (305) are fixedly connected to one ends of the first compression spring (202) and the second compression spring (304) respectively.

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