PCR reaction tube palm centrifugal set
By designing the specific structure of the centrifuge and orifice plate, the problem that the existing 96-well plate cannot be filled with 200ul centrifuge tubes is solved, and efficient PCR experimental operation is achieved, suitable for large-scale sample processing.
Patent Information
- Application Number
- CN202422257328.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-14
- Publication Date
- 2025-07-29
- Estimated Expiration
- 2034-09-14
AI Technical Summary
The existing 96-well plate has a small hole spacing, which makes it impossible to fill the open-cover 200ul centrifuge tube, which cannot meet the needs of large-scale PCR experiments.
A PCR reaction tube palm centrifugal set is designed, including centrifuge and orifice plate. The pallet and sub-plate are designed so that the center distance of adjacent mounting holes is 17~25mm, suitable for 200ul centrifuge tubes, and the fixability is enhanced through the lug structure, and the magnetic suction plate is used to achieve stable storage of sub-plate.
It is realized that all the installation holes of each partition plate can be filled with open-covered 200ul centrifuge tubes, which improves work efficiency and is suitable for large-scale PCR experimental operations, simplifies the operation process, and reduces the probability of error sampling.
Smart Images
Figure CN223159411U_ABST
Abstract
Description
Technical Field
[0001] This application relates to the technical field of centrifuges, and specifically relates to a palm centrifuge set for PCR reaction tubes. Background Art
[0002] The PCR diagnostic technology, also known as the polymerase chain reaction technology, has the advantages of high specificity, strong sensitivity, and simple operation. In today's rapid development of life technology science, the PCR technology has become the most conventional technology in the field of molecular biology. People often apply this technology to fields such as food, microbiology, and biological research.
[0003] During the daily ordinary PCR experiment process, it is often necessary to first add various reaction solutions into 200ul centrifuge tubes and then place them on a PCR instrument for reaction. Due to different sample loading, various reaction solutions sometimes gather at the bottom of the centrifuge tube and sometimes on the centrifuge tube wall. Before loading onto the machine, it is necessary to fully mix all the liquids without leaving any bubbles, which requires an additional centrifugation step before loading onto the machine.
[0004] The commercially available 96-well plate can hold 96 200ul centrifuge tubes with snap-on lids. Since the hole diameter is 7mm and the center distance between adjacent holes is only 9mm, and the maximum length of the upper surface of the open centrifuge tube reaches 12mm, this 96-well plate cannot be filled with 96 open 200ul centrifuge tubes. For large-scale experiments, multiple 96-well plates need to be prepared before sample loading, and at most only 32 samples of 200ul centrifuge tubes can be centrifuged at a time, which is not suitable for large-scale PCR experiment operations. Summary of the Utility Model
[0005] In view of the problem that the small hole spacing of the well plate results in the inability to fill the open 200ul centrifuge tubes (PCR reaction tubes), the following technical solutions are proposed in this application.
[0006] A palm centrifuge set for PCR reaction tubes includes a centrifuge and a well plate.
[0007] The centrifuge has a rotating shaft and two trays. The two trays are symmetrically fixed on the rotating shaft.
[0008] The well plate has two identical sub-plates. The sub-plates can be adaptively inserted into the trays.
[0009] Each sub-plate has a plurality of mounting holes arranged in a horizontal and vertical array. The aperture of each mounting hole is equal and is used to adaptively insert a PCR reaction tube. Horizontally and vertically, the center distance between adjacent mounting holes is equal, and the center distance between adjacent mounting holes is 17 - 25mm.
[0010] By adopting the above technical solution, two identical sub-boards are installed on two symmetrical pallets, balancing the centrifugal weight and making the centrifugation stable. The center distance between the adjacent mounting holes of each sub-board is 17 - 25 mm, which is greater than the upper end length of the 200 μl centrifuge tube with the lid open. Then, all the mounting holes of each sub-board can be filled with the 200 μl centrifuge tubes with the lid open, with high working efficiency and being suitable for large-scale PCR experiment operations.
[0011] A preferred structure of the PCR reaction tube hand-held centrifugation set is that the aperture of the mounting hole is 6 - 8 mm.
[0012] By adopting the above technical solution, a 200 μl centrifuge tube can be properly placed in this aperture.
[0013] A preferred structure of the PCR reaction tube hand-held centrifugation set is that there are first lugs on both sides of the upper end of each pallet. There are second lugs on both sides of the upper end of each sub-board. The two second lugs can be adaptively inserted into the two first lugs.
[0014] By adopting the above technical solution, by clamping the second lug into the first lug, the restraint of the pallet on the sub-board is strengthened, reducing the probability of the sub-board detaching from the pallet.
[0015] A preferred structure of the PCR reaction tube hand-held centrifugation set is that the upper surface and the lower surface of the pallet are parallel, and the angle between the upper surface of the pallet and the horizontal plane is 50° - 70°.
[0016] By adopting the above technical solution, the liquid on the tube wall after centrifugation can be flung to the bottom of the centrifuge tube.
[0017] A preferred structure of the PCR reaction tube hand-held centrifugation set is that the well plate further includes a base. The two sub-boards can be adaptively installed on the base.
[0018] By adopting the above technical solution, the two sub-boards are stored.
[0019] A preferred structure of the PCR reaction tube hand-held centrifugation set is that the well plate has a magnetic plate, and the magnetic plate is vertically fixed on the base. A magnetic object is installed on one side of each sub-board. The magnetic object can be adsorbed by the magnetic plate.
[0020] By adopting the above technical solution, the sub-board can be fixed on the base, facilitating storage.
[0021] A preferred structure of the PCR reaction tube hand-held centrifugation set is that each sub-board has 48 mounting holes, 12 horizontally and 4 rows vertically.
[0022] By adopting the above technical solution, the two sub-plates can replace a 96-well plate on the market, and all the mounting holes of each sub-plate can be filled with opened 200ul centrifuge tubes, which has high work efficiency and is suitable for large-scale PCR experimental operations.
[0023] A preferred structure of the PCR reaction tube handheld centrifuge kit includes the centrifuge further comprising a base, a start switch, a speed adjustment knob, a cover, a spring, and a plug. The start switch and the speed adjustment knob are both mounted on the base, the plug is connected to the base, and the spring is fixed to the base.
[0024] The lower end of the rotating shaft is rotatably mounted in the machine base. The two supporting plates are located above the machine base.
[0025] The cover is rotatably mounted on the machine base and can be clamped on the elastic sheet to cover the two supporting plates.
[0026] By adopting the above technical solution, the centrifuge has a simple structure and is easy to operate.
[0027] The present invention's handheld centrifuge kit for PCR reaction tubes offers the following advantages: Two symmetrical support plates hold two identical centrifuge plates, balancing the centrifuge weight and ensuring stable centrifugation. The center-to-center distance between adjacent mounting holes on each centrifuge plate is 17-25 mm, which is greater than the upper length of an uncapped 200-ul centrifuge tube. This allows all mounting holes on each centrifuge plate to be fully loaded with uncapped 200-ul centrifuge tubes, resulting in high efficiency and suitability for large-scale PCR experiments. BRIEF DESCRIPTION OF THE DRAWINGS
[0028] Figure 1 Schematic diagram of the structure of the centrifuge.
[0029] Figure 2 A top view of the orifice plate.
[0030] Figure 3 This is a three-dimensional diagram of the orifice plate.
[0031] Figure numerals: 100, centrifuge; 200, orifice plate; 13, rotating shaft; 14, support plate; 2, first sub-plate; 3, second sub-plate; 6, mounting hole; 7, bottom support; 15, first lug; 4, second lug; 1, base; 5, magnetic plate; 8, machine base; 16, start switch; 9, speed adjustment knob; 11, cover; 12, shrapnel; 10, plug. DETAILED DESCRIPTION
[0032] The present application is further described below with reference to the accompanying drawings.
[0033] A handheld centrifuge set for PCR reaction tubes comprises a centrifuge 100 and a well plate 200.
[0034] like Figure 1 The centrifuge 100 comprises a rotating shaft 13 and two supporting plates 14. The two supporting plates 14 are symmetrically fixed on the rotating shaft 13. The supporting plates 14 may be a box body with an open top.
[0035] like Figure 2 The well plate 200 has two identical sub-plates, namely a first sub-plate 2 and a second sub-plate 3. The sub-plates are similar in structure to the common 96-well plate 200 and can be adapted to be installed in the support plate 14.
[0036] Each of the sub-plates has a plurality of mounting holes 6 arranged in a horizontal and vertical array. Each sub-plate has a bracket adapted to fit a PCR reaction tube, the upper opening of each bracket forming a mounting hole 6, and the bottom of the bracket being a base 7 smaller than the mounting hole 6.
[0037] Each mounting hole 6 has a uniform diameter, ranging from 6 to 8 mm, for example, 7 mm, to accommodate a 200 μl PCR reaction tube. The distance between the centers of adjacent mounting holes 6 is equal in both the horizontal and vertical directions, ranging from 17 to 25 mm to accommodate the opened lid of a 200 μl PCR reaction tube.
[0038] In the aforementioned handheld centrifuge kit, two symmetrical support plates 14 hold two identical splitter plates, providing balance and ensuring stable centrifugation. The center-to-center distance between adjacent mounting holes 6 on each splitter plate is 17-25 mm, which is greater than the upper length of an uncapped 200-ul centrifuge tube. This allows all mounting holes 6 on each splitter plate to be fully loaded with uncapped 200-ul centrifuge tubes, resulting in high efficiency and suitability for large-scale PCR experiments.
[0039] To reduce the chance of the split plate detaching from the support plate 14 during centrifugation, the present application features first lugs 15 on both sides of the upper end of each support plate 14. Each split plate also features second lugs 4 on both sides of the upper end. The two second lugs 4 fit snugly within the two first lugs 15. The first lugs 15 engaging the second lugs 4 strengthens the support plate 14's grip on the split plate, reducing the chance of the split plate detaching from the support plate 14 during centrifugation.
[0040] The upper and lower surfaces of the support plates 14 are parallel, and the angle between the upper surfaces of the support plates 14 and the horizontal plane is 50° to 70°, for example, 60°. The distance between the upper ends of the two support plates 14 is greater than the distance between the lower ends, and the two support plates 14 are in an upward leaf shape. This structure allows the liquid on the tube wall to be thrown to the bottom of the centrifuge tube after centrifugation.
[0041] like Figure 3 The orifice plate 200 further includes a base 1. The two sub-plates can be adapted to be mounted on the base 1, and the two sub-plates are accommodated.
[0042] The orifice plate 200 has a magnetic attraction plate 5, and the magnetic attraction plate 5 is vertically fixed on the base 1. A magnetic object is installed on one side of each sub-plate, and the magnetic object can be an iron sheet or the like. The magnetic object can be adsorbed by the magnetic attraction plate 5. Then the two sub-plates can be fixed on both sides of the magnetic attraction plate 5, which is convenient for storage.
[0043] Each sub-plate can have 48 mounting holes 6, 12 horizontally and 4 rows vertically. Then the two sub-plates can replace a 96-well plate 200 on the market, and all the mounting holes 6 of each sub-plate can be filled with 200ul centrifuge tubes with open caps, with high working efficiency and suitable for a large number of PCR experiment operations.
[0044] For the above detachable 96-well plate 200, the hole pitch is increased, and up to 96 200ul centrifuge tubes with open caps can be placed, which is convenient for sample addition, simplifies the operation process, reduces the occurrence of incorrect sample addition, and improves the working efficiency.
[0045] The centrifuge 100 further includes a machine base 8, a start switch 16, a speed adjustment knob 9, a cover 11, a spring piece 12 and a plug 10. The start switch 16 and the speed adjustment knob 9 are both installed on the machine base 8, and the plug 10 is connected to the machine base 8. The spring piece 12 is fixed on the machine base 8.
[0046] The lower end of the rotating shaft 13 is rotatably installed in the machine base 8. Two support plates 14 are located above the machine base 8.
[0047] The cover 11 is rotatably installed on the machine base 8. The cover 11 can be stuck on the spring piece 12 to cover the two support plates 14.
[0048] The centrifuge 100 is equipped with a button for adjusting the rotation speed, and the experimenter can adjust the rotation speed as needed. The centrifuge 100 has a simple structure and is convenient to operate.
[0049] The drawings of the present application are schematic diagrams composed of main components, and do not limit the only external dimensions, connection methods, assembly forms, positional relationships, etc. of the present application. Some rods and the like are omitted in the drawings.
[0050] It should be noted that the multiple solutions provided in the present application include their basic solutions, which are independent of each other and do not restrict each other. However, they can also be combined with each other without conflict to jointly achieve multiple effects.
[0051] The above shows and describes the basic principles, main features and advantages of the present application. Those skilled in the art should understand that the present application is not limited by the above embodiments. Without departing from the essence of the present application, the present application can also be optimized and improved, and these optimizations and improvements all fall within the scope of protection.
Claims
1. A PCR reaction tube hand-held centrifugation set, characterized in that, It includes a centrifuge (100) and a microplate (200). The centrifuge (100) has a rotating shaft (13) and two trays (14); the two trays (14) are symmetrically fixed on the rotating shaft (13). The microplate (200) has two identical sub - plates; the sub - plates can be fitted into the trays (14). Each sub - plate has a plurality of mounting holes (6) arranged in a horizontal and vertical array; the aperture of each mounting hole (6) is equal, for fitting and inserting a PCR reaction tube; in the horizontal and vertical directions, the center - to - center distance between adjacent mounting holes (6) is equal, and the center - to - center distance between adjacent mounting holes (6) is 17 - 25 mm.
2. The PCR reaction tube handheld centrifugation set according to claim 1, characterized in that, The aperture of the mounting hole (6) is 6 - 8 mm.
3. The PCR reaction tube handheld centrifugation set according to claim 1, characterized in that, On both sides of the upper end of each tray (14), there are first lugs (15); on both sides of the upper end of each sub - plate, there are second lugs (4); the two second lugs (4) can be fitted into the two first lugs (15).
4. The PCR reaction tube hand-held centrifugation set according to claim 1, characterized in that, The upper surface and the lower surface of the tray (14) are parallel, and the angle between the upper surface of the tray (14) and the horizontal plane is 50° - 70°.
5. The PCR reaction tube handheld centrifuge set according to claim 1, wherein, The microplate (200) further includes a base (1); the two sub - plates can be fitted and mounted on the base (1).
6. The PCR reaction tube hand-held centrifugation set according to claim 5, wherein, The microplate (200) has a magnetic plate (5), the magnetic plate (5) is vertically fixed on the base (1); on one side of each sub - plate, there is a magnetic object; the magnetic object can be adsorbed by the magnetic plate (5).
7. The PCR reaction tube hand-held centrifugation set according to claim 1, characterized in that, Each sub - plate has 48 mounting holes (6), 12 in the horizontal direction and 4 rows in the vertical direction.
8. The PCR reaction tube hand-held centrifugation set according to claim 1, characterized in that, The centrifuge (100) further includes a machine base (8), a start switch (16), a speed - regulating knob (9), a cover (11), a spring piece (12) and a plug (10); the start switch (16) and the speed - regulating knob (9) are both mounted on the machine base (8), the plug (10) is connected to the machine base (8); the spring piece (12) is fixed on the machine base (8). The lower end of the rotating shaft (13) is rotatably mounted in the machine base (8); the two trays (14) are located above the machine base (8). The cover (11) is rotatably mounted on the machine base (8); the cover (11) can be stuck on the spring piece (12) to cover the two trays (14).