Simple and convenient device for rapidly screening mature stage VI oocytes of xenopus laevis

Abstract

The utility model relates to the technical field of cell screening, and discloses a simple and convenient device for rapidly screening mature period VI oocytes of Xenopus laevis, which comprises a transparent plastic cup, a removable mounting rack is arranged in an inner cavity of the transparent plastic cup, and an upper-layer screen and a lower-layer screen are sequentially arranged on the mounting rack from top to bottom. The upper-layer screen and the lower-layer screen are arranged in parallel, broken mature period VI oocyte cells can be conveniently left on the upper-layer screen through the upper-layer screen, oocytes below mature period V can be left at the bottom of the plastic cup through the screen, mature period VI oocyte cells needed by experiments can be left between the two layers of screens, and the oocytes below the mature period V can be left in the middle of the two layers of screens. The middle oocytes are carefully sucked by a rubber head dropper by removing the upper-layer screen, so that the mature period VI oocytes of the Xenopus laevis can be quickly screened, the mature period VI oocytes of the Xenopus laevis required by experiments can be quickly and efficiently screened, and time and labor are saved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell screening, in particular to a simple device for rapidly screening mature stage VI oocytes of Xenopus laevis. Background Technique

[0002] Xenopus laevis is a chordate of the genus Xenopus in the family Pipidae. Xenopus laevis has a plump and flat body, a pointed head in a streamlined shape, and both eyes and nostrils facing upwards. The hind limbs of Xenopus laevis are very developed, with five toes, and there is a well-developed web between the toes. Among them, the three toes located on the inner side have keratinized claws at the ends; the mature eggs of Xenopus laevis have a clear vegetal pole and animal pole. Fertilization causes cortical movement. The Xenopus laevis embryo hatches into a larva after stages such as cleavage, blastula, gastrula, neurula, and tail bud stage; the hind limbs of the tadpole begin to develop after about 5 days and gradually enter the metamorphosis stage, and the metamorphosis is completed in two months. The larvae need to grow for 1 to 2 years to reach sexual maturity; the oocytes of Xenopus laevis are really excellent materials for biological experiments. In addition to constructing an oocyte expression system, they are also commonly used in the research of cell division and cell cycle regulation;

[0003] At present, the acquisition of a single mature stage VI oocyte of Xenopus laevis mainly relies on manual methods. After the frog eggs dissected from Xenopus laevis are digested by collagenase, there are problems such as different sizes and breakages. It is necessary to manually select suitable oocytes under a stereomicroscope with tweezers according to the standards of non-damaged, uniform size and plumpness, and mature stage VI oocytes. At present, the manual selection of mature stage VI oocytes of Xenopus laevis mainly requires the experimenter to stay in front of the stereomicroscope for a long time for operation, which has the disadvantages of time-consuming and laborious. Oocytes with different shapes, sizes and degrees of breakage placed together will also cause visual fatigue. Therefore, we need to propose a simple device for rapidly screening mature stage VI oocytes of Xenopus laevis. Content of the Utility Model

[0004] The purpose of the utility model is to provide a simple device for rapidly screening mature stage VI oocytes of Xenopus laevis, which is convenient for rapidly screening mature stage VI oocytes of Xenopus laevis, reduces the working intensity of experimenters staying in front of the stereomicroscope for a long time to manually select mature stage VI oocytes of Xenopus laevis, and further improves the efficiency of screening mature stage VI oocytes of Xenopus laevis, so as to solve the problems raised in the above background technique.

[0005] To achieve the above object, the present utility model provides the following technical solutions: A simple device for quickly screening mature stage VI oocytes of Xenopus laevis, including a transparent plastic cup, wherein an installable and removable mounting frame is arranged in the inner cavity of the transparent plastic cup. An upper screen and a lower screen are sequentially arranged on the mounting frame from top to bottom. The upper screen and the lower screen are arranged in parallel. The upper screen is 12 mesh, and the lower screen is 16 mesh. A limiting mechanism for clamping the upper screen and the lower screen is installed on the mounting frame.

[0006] Preferably, upper side plates are fixedly connected to the four peripheries of the upper surface of the upper screen, and upper rectangular holes for clamping the limiting mechanism are symmetrically opened on the outer sides of the upper side plates.

[0007] Preferably, lower side plates are fixedly connected to the four peripheries of the upper surface of the lower screen, and lower rectangular holes for clamping the limiting mechanism are symmetrically opened on the outer sides of the lower side plates.

[0008] Preferably, the mounting frame includes a bottom frame and four groups of vertical rods. The four groups of vertical rods are respectively fixedly connected to the four corners of the upper surface of the bottom frame. An upper limiting plate and a lower limiting plate are sequentially fixedly connected between adjacent two groups of vertical rods from top to bottom.

[0009] Preferably, upper through holes for the limiting mechanism to penetrate are opened on the opposite sides of the two groups of relatively arranged upper limiting plates, and lower through holes for the limiting mechanism to penetrate are opened on the opposite sides of the two groups of relatively arranged lower limiting plates.

[0010] Preferably, the limiting mechanism includes a side block. A horizontal rod is arranged above the side block. A limiting block is arranged on one side of the side block. Two sets of telescopic sleeve rods are arranged between the limiting block and the side block. Second return springs are sleeved on the outer sides of the two sets of telescopic sleeve rods.

[0011] Preferably, two sets of first columns, two sets of second columns and a pressing rod are fixedly connected to the lower surface of the horizontal rod. A pressing part is integrally formed on one side of the horizontal rod. The lower end of the pressing rod is fixedly connected with a pressing plate. A first return spring is arranged on the lower surface of the pressing plate. A jack for inserting the second column is opened on the upper surface of the side block. Triangular push blocks are fixedly connected to the lower ends of the two sets of first columns. A stepped hole for the sliding connection of a push plate is opened on the upper surface of the side block. The cross section of the side block is arranged in a C shape. One end of the telescopic sleeve rod is fixedly connected to the inner cavity of the side block.

[0012] Preferably, both ends of one side of the limit block are fixedly connected with side plates that are slidably connected to the side blocks. On one side of the two side plates, sealing plates located on both sides of the side block are fixedly connected. On the opposite sides of the two sealing plates, triangular abutting blocks that are slidably connected to the triangular sliders are fixedly connected. A rounded corner part is arranged at the upper end of the other side of the limit block. The other side of the telescopic sleeve rod is fixedly connected to one side of the limit block.

[0013] Preferably, a plurality of reinforcing connecting rods are fixedly connected to the inner cavity of the bottom frame. Rotating rods are rotatably connected to the upper ends of two oppositely arranged vertical rods. Opposite ends of the two rotating rods are fixedly connected with pull rings.

[0014] Preferably, upper support rods are fixedly connected to both ends of the opposite sides of the two upper limiting plates. Upper cushion blocks for supporting the upper sieve mesh are adhesively bonded to the upper surfaces of the two upper support rods. Lower support rods are fixedly connected to both ends of the opposite sides of the two lower limiting plates. Lower cushion blocks for supporting the lower sieve mesh are fixedly connected to the upper surfaces of the two lower support rods.

[0015] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0016] The present utility model mainly realizes the cooperation among the upper sieve mesh, the lower sieve mesh and the transparent plastic cup. Through the upper sieve mesh, it is convenient to leave the damaged mature stage VI oocytes on the upper sieve mesh. The oocytes below mature stage V will pass through the sieve mesh and remain at the bottom of the plastic cup. The mature stage VI oocytes required for the experiment will remain in the middle of the double-layer sieve mesh. By removing the upper sieve mesh and carefully sucking the middle oocytes with a dropper, the mature stage VI oocytes of Xenopus laevis can be quickly screened, and the mature stage VI oocytes of Xenopus laevis required for the experiment can be quickly and efficiently screened, saving time and effort. Description of the Drawings

[0017] Figure 1 is the overall structural schematic diagram of the present utility model;

[0018] Figure 2 is the structural schematic diagram of the mounting frame of the present utility model;

[0019] Figure 3 is the structural schematic diagram of the upper sieve mesh of the present utility model;

[0020] Figure 4 is the structural schematic diagram of the lower sieve mesh of the present utility model;

[0021] Figure 5 is the structural schematic diagram of the limiting mechanism of the present utility model.

[0022] In the figure: 1, transparent plastic cup; 2, mounting rack; 21, bottom frame; 22, vertical rod; 23, pull ring; 24, rotating rod; 25, upper limiting plate; 26, lower limiting plate; 27, upper support rod; 28, lower support rod; 29, upper cushion block; 210, lower cushion block; 211, upper through hole; 212, lower through hole; 213, strengthening connecting rod; 3, limiting mechanism; 31, side block; 311, jack; 312, stepped hole; 32, horizontal rod; 321, pressing part; 322, first column; 323, triangular push block; 324, pressing rod; 325, pressing plate; 326, first return spring; 33, limiting block; 331, rounded corner part; 332, side plate; 333, sealing plate; 334, triangular abutting block; 35, telescopic sleeve rod; 36, second return spring; 4, upper sieve mesh; 41, upper surrounding plate; 42, upper rectangular hole; 5, lower sieve mesh; 51, lower surrounding plate; 52, lower rectangular hole. Detailed implementation mode

[0023] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work shall fall within the protection scope of the present invention.

[0024] Please refer to Figures 1-5 , the present invention provides a technical solution: a simple device for quickly screening mature stage VI oocytes of Xenopus laevis, including a transparent plastic cup 1. An accessible mounting rack 2 is arranged in the inner cavity of the transparent plastic cup 1. An upper sieve mesh 4 and a lower sieve mesh 5 are sequentially arranged on the mounting rack 2 from top to bottom. The upper sieve mesh 4 and the lower sieve mesh 5 are arranged in parallel. The upper sieve mesh 4 is 12 mesh, and the lower sieve mesh 5 is 16 mesh. A limiting mechanism 3 for clamping the upper sieve mesh 4 and the lower sieve mesh 5 is installed on the mounting rack 2.

[0025] The upper surrounding plate 41 is fixedly connected to the periphery of the upper surface of the upper sieve mesh 4. Upper rectangular holes 42 for clamping by the limiting mechanism 3 are symmetrically arranged on the outer side of the upper surrounding plate 41. Through the upper rectangular holes 42, it is convenient for the limiting mechanism 3 to pass through and clamp the upper surrounding plate 41, thereby realizing the fixation of the upper sieve mesh 4. Both the upper sieve mesh 4 and the lower sieve mesh 5 are detachable for easy cleaning.

[0026] The lower surrounding plate 51 is fixedly connected to the periphery of the upper surface of the lower sieve mesh 5. Lower rectangular holes 52 for clamping by the limiting mechanism 3 are symmetrically arranged on the outer side of the lower surrounding plate 51. Through the lower limiting plate 26, it is convenient to limit the lower surrounding plate 51 on which the lower sieve mesh 5 is installed, thereby improving the disassembly and assembly efficiency of the lower sieve mesh 5.

[0027] The mounting frame 2 includes a bottom frame 21 and four groups of vertical rods 22. The four groups of vertical rods 22 are respectively fixedly connected to the four corners of the upper surface of the bottom frame 21. An upper limit plate 25 and a lower limit plate 26 are fixedly connected in sequence from top to bottom between two adjacent groups of vertical rods 22. The overall stability and strength of the mounting frame 2 are improved through the bottom frame 21 and the vertical rods 22, thereby increasing the service life and preventing the mounting frame 2 from deforming.

[0028] Upper through holes 211 for the limiting mechanism 3 to pass through are formed on the opposite sides of two relatively arranged upper limit plates 25, and lower through holes 212 for the limiting mechanism 3 to pass through are formed on the opposite sides of two relatively arranged lower limit plates 26. Through the upper through holes 211 and the lower through holes 212, it is convenient for the limiting block 33 to pass through and fix the upper enclosure plate 41 and the lower enclosure plate 51, so as to facilitate the fixing of the upper screen 4 and the lower screen 5.

[0029] The limiting mechanism 3 includes a side block 31. A horizontal rod 32 is arranged above the side block 31. A limiting block 33 is arranged on one side of the side block 31. Two sets of telescopic sleeve rods 35 are arranged between the limiting block 33 and the side block 31. Second return springs 36 are sleeved on the outer sides of the two sets of telescopic sleeve rods 35. Through the second return springs 36, it is convenient for the limiting block 33 to be quickly clamped into the upper rectangular hole 42 and the lower rectangular hole 52, improving the installation efficiency. At the same time, it is convenient for the limiting block 33 to quickly reset after disassembly.

[0030] Two sets of first vertical columns 322, two sets of second vertical columns and a pressing rod 324 are fixedly connected to the lower surface of the horizontal rod 32. A pressing part 321 is integrally formed on one side of the horizontal rod 32. A pressing plate 325 is fixedly connected to the lower end of the pressing rod 324. A first return spring 326 is arranged on the lower surface of the pressing plate 325. A jack 311 for the second vertical column to be inserted is formed on the upper surface of the side block 31. Triangular push blocks 323 are fixedly connected to the lower ends of the two sets of first vertical columns 322. A stepped hole 312 for the push plate to slide is formed on the upper surface of the side block 31. The cross section of the side block 31 is in a C-shaped arrangement. One end of the telescopic sleeve rod 35 is fixedly connected to the inner cavity of the side block 31. By inserting the second vertical column into the jack 311, the stability of the horizontal rod 32 when being pressed is improved.

[0031] Both ends of one side of the limit block 33 are fixedly connected with side plates 332 that are slidably connected to the side block 31. One side of the two groups of side plates 332 is fixedly connected with sealing plates 333 located on both sides of the side block 31. One side of the two groups of sealing plates 333 is fixedly connected with triangular abutting blocks 334 that are slidably connected to the triangular sliders. The upper end of the other side of the limit block 33 is provided with a rounded corner portion 331. The other side of the telescopic sleeve rod 35 is fixedly connected to one side of the limit block 33. The layer board facilitates the closing of the opening of the side block 31, thereby preventing the solution from invading and corroding the second return spring 36. At the same time, by the triangular push block 323 abutting against the triangular abutting block 334, it is convenient to adjust the position of the limit block 33, so as to quickly disassemble the upper sieve mesh 4 and the lower sieve mesh 5 in one direction.

[0032] A plurality of reinforcing connecting rods 213 are fixedly connected to the inner cavity of the bottom frame 21. The upper ends of the two relatively arranged vertical rods 22 are rotatably connected with rotating rods 24. The opposite ends of the two rotating rods 24 are fixedly connected with a pull ring 23. The pull ring 23 is convenient to rotate through the rotating rod 24, thereby facilitating the overall placement and removal of the mounting frame 2 in the transparent plastic cup 1, providing convenience.

[0033] Both ends of the relative sides of the two groups of upper limit plates 25 are fixedly connected with upper support rods 27. The upper surfaces of the two groups of upper support rods 27 are bonded with upper cushion blocks 29 for supporting the upper sieve mesh 4. Both ends of the relative sides of the two groups of lower limit plates 26 are fixedly connected with lower support rods 28. The upper surfaces of the two groups of lower support rods 28 are fixedly connected with lower cushion blocks 210 for supporting the lower sieve mesh 5. The upper sieve mesh 4 and the lower sieve mesh 5 are supported by the upper support rods 27 and the lower support rods 28, respectively, to prevent the upper sieve mesh 4 and the lower sieve mesh 5 from deforming during filtration.

[0034] During use, the user first installs the upper sieve mesh 4 between the two groups of upper limit plates 25 and engages it through the limiting mechanism 3. At the same time, the lower sieve mesh 5 is arranged between the two groups of lower sieve meshes 5 and engaged through the limiting mechanism 3 to complete the installation of the double-layer sieve mesh. Among them, the upper sieve mesh 4 has a diameter of 6 cm and 12 meshes, and the lower sieve mesh 5 has a diameter of 6 cm and 16 meshes. After the installation is completed, the mounting frame 2 is installed into a cylindrical transparent plastic cup 1 with a diameter of 6 cm and a height of 10 cm. After the device is installed, pour the pre-prepared Ga 2+- Pour the -free solution to the cup mouth to submerge the upper sieve 4. Carefully place the Xenopus laevis oocytes on the upper sieve 4 with a dropper. Depending on the maturity and damage degree of the frog oocytes, their sizes will vary. Generally, the diameter of the damaged stage VI oocytes is larger than that of the stage VI oocytes. The diameter of the stage VI oocytes is generally 1200 - 1300 μm, and the diameter of the oocytes below stage V is generally less than 1000 μm. Therefore, the damaged stage VI oocytes will remain on the upper sieve 4, and the oocytes below stage V will pass through the sieve and remain at the bottom of the plastic cup. The stage VI oocytes required for the experiment will remain in the middle of the double sieve. By removing the upper sieve 4 and carefully sucking the middle oocytes with a dropper, the stage VI Xenopus laevis oocytes can be quickly screened.

[0035] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principle and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A simple device for rapidly screening mature stage VI oocytes of Xenopus laevis, comprising a transparent plastic cup (1), characterized in that: Inside the cavity of the transparent plastic cup (1), there is a removable mounting rack (2). On the mounting rack (2), a upper sieve mesh (4) and a lower sieve mesh (5) are arranged in sequence from top to bottom. The upper sieve mesh (4) and the lower sieve mesh (5) are arranged in parallel. The upper sieve mesh (4) is 12 mesh, and the lower sieve mesh (5) is 16 mesh. A limiting mechanism (3) for clamping the upper sieve mesh (4) and the lower sieve mesh (5) is installed on the mounting rack (2).

2. The simple device for rapidly screening mature stage VI oocytes of Xenopus laevis according to claim 1, wherein: Around the upper surface of the upper sieve mesh (4), an upper enclosing plate (41) is fixedly connected. On the outer side of the upper enclosing plate (41), upper rectangular holes (42) for the limiting mechanism (3) to be clamped are symmetrically opened.

3. A simple device for rapidly screening mature stage VI oocytes of Xenopus laevis, characterized in that: Around the upper surface of the lower sieve mesh (5), a lower enclosing plate (51) is fixedly connected. On the outer side of the lower enclosing plate (51), lower rectangular holes (52) for the limiting mechanism (3) to be clamped are symmetrically opened.

4. A simple device for rapidly screening mature stage VI oocytes of Xenopus laevis, characterized in that: The mounting rack (2) includes a bottom frame (21) and four groups of vertical rods (22). The four groups of vertical rods (22) are respectively fixedly connected to the four corners of the upper surface of the bottom frame (21). Between adjacent two groups of vertical rods (22), an upper limiting plate (25) and a lower limiting plate (26) are fixedly connected in sequence from top to bottom.

5. A simple device for rapidly screening mature stage VI oocytes of Xenopus laevis, characterized in that: On the opposite sides of the two groups of upper limiting plates (25) arranged oppositely, upper through holes (211) for the limiting mechanism (3) to penetrate are opened. On the opposite sides of the two groups of lower limiting plates (26) arranged oppositely, lower through holes (212) for the limiting mechanism (3) to penetrate are opened.

6. The simple device for rapidly screening mature stage VI oocytes of Xenopus laevis according to claim 5, wherein: The limiting mechanism (3) includes a side block (31). Above the side block (31), there is a horizontal rod (32). On one side of the side block (31), there is a limiting block (33). Between the limiting block (33) and the side block (31), two groups of telescopic sleeve rods (35) are arranged. On the outer sides of the two groups of telescopic sleeve rods (35), second return springs (36) are sleeved.

7. A simple device for rapidly screening mature stage VI oocytes of Xenopus laevis, characterized in that: On the lower surface of the horizontal rod (32), two groups of first vertical columns (322), two groups of second vertical columns and a pressing rod (324) are fixedly connected. On one side of the horizontal rod (32), a pressing part (321) is integrally formed. At the lower end of the pressing rod (324), a pressing plate (325) is fixedly connected. On the lower surface of the pressing plate (325), a first return spring (326) is arranged. On the upper surface of the side block (31), a jack (311) for the second vertical column to be inserted is opened. At the lower ends of the two groups of first vertical columns (322), triangular pushing blocks (323) are fixedly connected. On the upper surface of the side block (31), a stepped hole (312) for the pushing plate to slide is opened. The cross section of the side block (31) is in a shape of a reversed C. One end of the telescopic sleeve rod (35) is fixedly connected to the inner cavity of the side block (31).

8. A simple device for rapidly screening mature stage VI oocytes of Xenopus laevis, characterized in that: On both ends of one side of the limiting block (33), side plates (332) slidably connected to the side block (31) are fixedly connected. On one side of the two groups of side plates (332), sealing plates (333) located on both sides of the side block (31) are fixedly connected. On the opposite sides of the two groups of sealing plates (333), triangular abutting blocks (334) slidably connected to the triangular slider are fixedly connected. On the upper end of the other side of the limiting block (33), a rounded corner portion (331) is provided. On the other side of the telescopic sleeve rod (35), it is fixedly connected to one side of the limiting block (33).

9. A simple device for rapidly screening mature stage VI oocytes of Xenopus laevis, characterized in that: Inside the bottom frame (21), multiple groups of strengthening connecting rods (213) are fixedly connected. On the upper ends of two oppositely arranged vertical rods (22), rotating rods (24) are rotatably connected. At the opposite ends of the two groups of rotating rods (24), a pull ring (23) is fixedly connected.

10. A simple device for rapidly screening mature stage VI oocytes of Xenopus laevis, characterized in that: On both ends of the opposite sides of the two groups of upper limiting plates (25), upper support rods (27) are fixedly connected. On the upper surfaces of the two groups of upper support rods (27), upper cushion blocks (29) for supporting the upper sieve mesh (4) are adhesively bonded. On both ends of the opposite sides of the two groups of lower limiting plates (26), lower support rods (28) are fixedly connected. On the upper surfaces of the two groups of lower support rods (28), lower cushion blocks (210) for supporting the lower sieve mesh (5) are fixedly connected.

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