Continuous centrifugal ultrafiltration treatment system for escherichia coli fermentation liquor

Abstract

The utility model discloses a treatment system for continuous centrifugal ultrafiltration of escherichia coli fermentation liquor. The treatment system comprises a fermentation tank, the disc centrifugal device is communicated with the downstream of the fermentation tank, and fermentation liquor in the fermentation tank enters the disc centrifugal device to be centrifuged; the one-step ultrafiltration device is communicated with the downstream of the disc centrifugal device, and supernate generated by centrifugation in the disc centrifugal device enters the one-step ultrafiltration device for ultrafiltration; the two-step ultrafiltration device is arranged at the downstream of the one-step ultrafiltration device in a communicating manner, the material liquid subjected to ultrafiltration in the one-step ultrafiltration device enters the two-step ultrafiltration device to be continuously subjected to ultrafiltration, and a concentrated solution is collected after ultrafiltration is finished; and the CIP online cleaning system is used for independently cleaning the fermentation tank, the disc centrifugal device and the liquid inlet pipeline thereof, the one-step ultrafiltration device and the liquid inlet pipeline thereof, and the two-step ultrafiltration device and the liquid inlet pipeline thereof.

Description

Technical Field

[0001] The utility model relates to the technical field of Escherichia coli fermentation, and particularly relates to a treatment system for continuous centrifugation and ultrafiltration of Escherichia coli fermentation broth. Background Technique

[0002] Since the development of genetic engineering technology to date, the exogenous protein expression system has expanded from the Escherichia coli prokaryotic expression system to eukaryotic expression systems such as yeast, insect cells, plants, and mammalian cells. Compared with other expression systems, the Escherichia coli expression system not only has the advantages of thorough genetic background, easy cultivation, simple genetic engineering operation, high efficiency, fast reproduction speed, and low cost, but also has a higher exogenous protein expression level; based on the above advantages, the Escherichia coli expression system is still one of the most widely used recombinant protein expression systems at present.

[0003] The traditional treatment process of Escherichia coli fermentation broth is as Figure 1 shown. The typical characteristic of the traditional treatment process is that intermediate products need to be temporarily stored and collected, which is a discontinuous production mode. And this discontinuous production mode generally has the following deficiencies: (1) The process flow is complex, which will reduce the product yield to a certain extent; (2) Proteins are generally sensitive to temperature, and the storage conditions of intermediate products are required to be high. The properties of proteins are easily affected during the storage process, and at the same time, the requirements for production verification are also increased; (3) The problem of on-line cleaning of process pipelines. Due to intermediate storage, there are cleaning dead corners in the on-line process of the liquid pipeline. Content of the Utility Model

[0004] The purpose of the utility model is: aiming at the problems existing in the existing treatment process system of Escherichia coli fermentation broth, such as the need for temporary storage and collection, which leads to complex process flow, low product yield, easy influence on the properties of proteins, and difficult cleaning, etc., a treatment system for continuous centrifugation and ultrafiltration of Escherichia coli fermentation broth is designed, which solves many problems brought by discontinuous production caused by temporary storage and collection.

[0005] In order to achieve the above purpose, the utility model is realized by the following technical scheme:

[0006] The utility model designs a treatment system for continuous centrifugation and ultrafiltration of Escherichia coli fermentation broth, and this treatment system includes:

[0007] A fermentation tank;

[0008] A disc centrifuge device, which is connected and arranged downstream of the fermentation tank, and the fermentation broth in the fermentation tank enters the disc centrifuge device for centrifugation;

[0009] A one-step ultrafiltration device, which is connected and arranged downstream of the disc centrifuge device, and the supernatant generated by centrifugation in the disc centrifuge device enters the one-step ultrafiltration device for ultrafiltration;

[0010] A two-step ultrafiltration device, which is connected and arranged downstream of the one-step ultrafiltration device. The permeate liquid after ultrafiltration in the one-step ultrafiltration device enters the two-step ultrafiltration device to continue ultrafiltration, and the concentrated liquid is collected after ultrafiltration ends.

[0011] And a CIP on-line cleaning system, which is used to independently clean the fermentation tank, the disc centrifuge device and its inlet pipeline, the one-step ultrafiltration device and its inlet pipeline, and the two-step ultrafiltration device and its inlet pipeline respectively.

[0012] Further, a treatment system for continuous centrifugation and ultrafiltration of Escherichia coli fermentation broth: The disc centrifuge device is connected and arranged with the fermentation tank through a first inlet pipe, and a valve V101 is arranged on the first inlet pipe.

[0013] Further, a treatment system for continuous centrifugation and ultrafiltration of Escherichia coli fermentation broth: The one-step ultrafiltration device is connected and arranged with the disc centrifuge device through a second inlet pipe, and a valve V102 is arranged on the second inlet pipe.

[0014] Further, a treatment system for continuous centrifugation and ultrafiltration of Escherichia coli fermentation broth: The two-step ultrafiltration device is connected and arranged with the one-step ultrafiltration device through a third inlet pipe, and a valve V103 is arranged on the third inlet pipe.

[0015] Further, a treatment system for continuous centrifugation and ultrafiltration of Escherichia coli fermentation broth: The CIP on-line cleaning system includes: a CIP station for providing cleaning liquid, a first circulation pump, a return water / discharge pipeline, a second circulation pump, a conductivity detection device, a first cleaning pipe for independently cleaning the fermentation tank, a second cleaning pipe for independently cleaning the first inlet pipe and the disc centrifuge device, a third cleaning pipe for independently cleaning the second inlet pipe, a fourth cleaning pipe for independently cleaning the one-step ultrafiltration device, a fifth cleaning pipe for independently cleaning the third inlet pipe, and a sixth cleaning pipe for independently cleaning the two-step ultrafiltration device; The first circulation pump is arranged at the outlet of the CIP station, the second circulation pump and the conductivity detection device are arranged on the return water / discharge pipeline. One end of the return water / discharge pipeline is connected and arranged in parallel with the outlets of the fermentation tank, the disc centrifuge device, the one-step ultrafiltration device and the two-step ultrafiltration device, and the other end is connected and arranged in parallel with the CIP station and the discharge pipe. The cleaned liquid flows back to the CIP station or is discharged through the return water / discharge pipeline.

[0016] Furthermore, a processing system for continuous centrifugation and ultrafiltration of Escherichia coli fermentation broth: The first cleaning pipe connects the CIP station and the fermentation tank, and a valve V104 is also provided thereon. A valve V105 and a valve V106 are provided downstream of the outlet of the fermentation tank on the return water / discharge pipeline. One end of the first liquid inlet pipe is connected to the return water / discharge pipeline between the valve V105 and the valve V106, and the other end is connected to the disc centrifuge device.

[0017] Furthermore, a processing system for continuous centrifugation and ultrafiltration of Escherichia coli fermentation broth: One end of the second cleaning pipe is connected to the CIP station, and the other end is connected to the first liquid inlet pipe and is close to the connection point of the first liquid inlet pipe and the return water / discharge pipeline. A valve V107 is provided on the second cleaning pipe. A valve V108 is provided on the return water / discharge pipeline near the outlet of the disc centrifuge device.

[0018] Furthermore, a processing system for continuous centrifugation and ultrafiltration of Escherichia coli fermentation broth: One end of the third cleaning pipe is connected to the CIP station, and the other end is connected to the second liquid inlet pipe and the connection point is upstream of the valve V102. Valves V109 and V110 are provided on the third cleaning pipe; One end of the fourth cleaning pipe is connected to the third cleaning pipe between the valve V109 and the valve V110, and the other end is connected to the one-step ultrafiltration device. A valve V111 is provided on the fourth cleaning pipe; A valve V112 is also provided on the return water / discharge pipeline near the outlet of the one-step ultrafiltration device.

[0019] Furthermore, a processing system for continuous centrifugation and ultrafiltration of Escherichia coli fermentation broth: One end of the fifth cleaning pipe is connected to the fourth cleaning pipe and the connection point is upstream of the valve V111, and the other end is connected to the third liquid inlet pipe. A valve V113 is provided on the fifth cleaning pipe;

[0020] One end of the sixth cleaning pipe is connected to the fifth cleaning pipe and the connection point is upstream of the valve V113, and the other end is connected to the two-step ultrafiltration device. A valve V114 is provided on the sixth cleaning pipe; A valve V115 is also provided on the return water / discharge pipeline near the outlet of the two-step ultrafiltration device.

[0021] Furthermore, a processing system for continuous centrifugation and ultrafiltration of Escherichia coli fermentation broth: A valve V116 is provided on the return water / discharge pipeline near the inlet of the CIP station, and a valve V117 is provided on the discharge pipe.

[0022] The beneficial effects of the present utility model:

[0023] This processing system for continuous centrifugation and ultrafiltration of Escherichia coli fermentation broth designed by the utility model cancels the collection of intermediate products, realizes continuous production from the fermentation broth discharging from the tank to ultrafiltration, uses a disc centrifuge instead of a tubular centrifuge in the system equipment, cancels the collection and storage of intermediate products, greatly shortens the process flow, realizes a continuous production mode and an on-line cleaning mode, and thus solves the problems such as complex process flow, low product yield, easy influence on protein properties and difficult cleaning of process pipelines caused by the discontinuous production due to the temporary storage and collection of intermediate products. Brief Description of the Drawings

[0024] In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the accompanying drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings in the following description are only some embodiments of the present utility model. For those skilled in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0025] Figure 1 It is a process flow chart of the existing processing of Escherichia coli fermentation broth;

[0026] Figure 2 It is a schematic structural diagram of a processing system for continuous centrifugation and ultrafiltration of Escherichia coli fermentation broth designed by the present utility model.

[0027] Markings in the figure: 1 - fermentation tank, 2 - disc centrifugation device, 3 - first-step ultrafiltration device, 4 - second-step ultrafiltration device, 5 - first inlet pipe, 6 - second inlet pipe, 7 - third inlet pipe, 8 - CIP station, 9 - first circulation pump, 10 - return water / discharge pipeline, 11 - second circulation pump, 12 - conductivity detection device, 13 - first cleaning pipe, 14 - second cleaning pipe, 15 - third cleaning pipe, 16 - fourth cleaning pipe, 17 - fifth cleaning pipe, 18 - sixth cleaning pipe, 19 - discharge pipe. Detailed Embodiments

[0028] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, rather than all embodiments. The description of at least one exemplary embodiment below is actually only illustrative and in no way restrictive of the present utility model and its application or use. Based on the embodiments in the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present utility model.

[0029] In the description of the present utility model, it should be understood that the orientation or positional relationship indicated by terms such as "upper", "lower", "left", "right", "top", "bottom", etc. is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as a limitation to the present utility model. In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of such features. Moreover, the terms "first", "second", etc. are used to distinguish similar objects and do not necessarily have to be used to describe a specific order or sequence. It should be understood that such data can be interchanged under appropriate circumstances so that the embodiments of the present utility model described herein can be implemented in an order different from those illustrated or described herein.

[0030] Embodiment 1

[0031] As Figure 2 shown, in this Embodiment 1, a treatment system for continuous centrifugation and ultrafiltration of Escherichia coli fermentation broth is designed. This treatment system includes:

[0032] A fermentation tank 1, in which fermentation broth is produced by fermentation;

[0033] A disc centrifuge device 2, which is connected to the outlet of the fermentation tank 1 through a first inlet pipe 5. The fermentation broth produced by fermentation in the fermentation tank 1 enters the disc centrifuge device 2 through the first inlet pipe 5 for centrifugation, and a valve V101 is provided on the first inlet pipe 5;

[0034] A primary ultrafiltration device 3, which is connected to the outlet of the disc centrifuge device 2 through a second inlet pipe 6. The supernatant produced by centrifugation in the disc centrifuge device 2 enters the primary ultrafiltration device 3 through the second inlet pipe 6 for ultrafiltration, and a valve V102 is also provided on the second inlet pipe 6;

[0035] A secondary ultrafiltration device 4, which is connected to the outlet of the primary ultrafiltration device 3 through a third inlet pipe 7. The feed liquid in the permeate section after ultrafiltration in the primary ultrafiltration device 3 enters the secondary ultrafiltration device 4 through the third inlet pipe 7 for continuous ultrafiltration, and the concentrated liquid is collected after ultrafiltration is completed. A valve V103 is provided on the third inlet pipe 7;

[0036] And a CIP on-line cleaning system, which is used to independently clean the fermentation tank 1, the disc centrifuge device 2 and the first inlet pipe 5, the primary ultrafiltration device 3 and the second inlet pipe 6, and the secondary ultrafiltration device 4 and the third inlet pipe 7 respectively;

[0037] Among them, the CIP on-line cleaning system includes: a CIP station 8 for providing cleaning liquid, a first circulation pump 9, a return water / discharge pipeline 10, a second circulation pump 11, a conductivity detection device 12, a first cleaning pipe 13 for independently cleaning the fermentation tank 1, a second cleaning pipe 14 for independently cleaning the first inlet pipe 5 and the disc centrifuge device 2, a third cleaning pipe 15 for independently cleaning the second inlet pipe 6, a fourth cleaning pipe 16 for independently cleaning the first ultrafiltration device 3, a fifth cleaning pipe 17 for independently cleaning the third inlet pipe 7, and a sixth cleaning pipe 18 for independently cleaning the second ultrafiltration device 4;

[0038] The first circulation pump 9 is arranged at the outlet of the CIP station 8 to provide power for the circulation of the cleaning liquid. The second circulation pump 11 and the conductivity detection device 12 are arranged on the return water / discharge pipeline 10. One end of the return water / discharge pipeline 10 is connected in parallel with the first inlet pipe 5 at the outlet of the fermentation tank 1, the second inlet pipe 6 at the outlet of the disc centrifuge device 2, the third inlet pipe 7 at the outlet of the first ultrafiltration device 3, and the outlet of the second ultrafiltration device 4. The other end of the return water / discharge pipeline 10 is connected in parallel and communicated with the CIP station 8 and the discharge pipe 19. The cleaned liquid flows back to the CIP station 8 through the return water / discharge pipeline 10 or is discharged through the discharge pipe 19.

[0039] Specifically, in the above-mentioned Embodiment 1, the CIP station 8 and the fermentation tank 1 are connected through the first cleaning pipe 13, and a valve V104 is arranged on the first cleaning pipe 13. Valves V105 and V106 are arranged downstream of the outlet of the fermentation tank 1 on the return water / discharge pipeline 10. One end of the first inlet pipe 5 is connected to the return water / discharge pipeline 10 between the valves V105 and V106, and the other end is connected to the disc centrifuge device 2;

[0040] One end of the second cleaning pipe 14 is connected to the CIP station 8, and the other end is connected to the first inlet pipe 5 and is close to the connection point of the first inlet pipe 5 and the return water / discharge pipeline 10. A valve V107 is arranged on the second cleaning pipe 14, and a valve V108 is arranged on the return water / discharge pipeline 10 close to the outlet of the disc centrifuge device 2;

[0041] One end of the third cleaning pipe 15 is connected to the CIP station 8, and the other end is connected to the second inlet pipe 6 and the connection point is upstream of the valve V102. Valves V109 and V110 are arranged on the third cleaning pipe 15. One end of the fourth cleaning pipe 16 is connected to the third cleaning pipe 15 between the valves V109 and V110, and the other end is connected to the first ultrafiltration device 3. A valve V111 is arranged on the fourth cleaning pipe 16, and a valve V112 is also arranged on the return water / discharge pipeline 10 close to the outlet of the first ultrafiltration device 3;

[0042] One end of the fifth cleaning pipe 17 is connected to the fourth cleaning pipe 16, and the connection point is located upstream of the valve V111. The other end is connected to the third inlet pipe 7. A valve V113 is provided on the fifth cleaning pipe 17. One end of the sixth cleaning pipe 18 is connected to the fifth cleaning pipe 17, and the connection point is located upstream of the valve V113. The other end is connected to the two-step ultrafiltration device 4. A valve V114 is provided on the sixth cleaning pipe 18. A valve V115 is also provided on the return water / discharge pipeline 10 near the outlet of the two-step ultrafiltration device 4.

[0043] A valve V116 is provided on the return water / discharge pipeline 10 near the inlet of the CIP station 8. A valve V117 is provided on the discharge pipe 19.

[0044] Online cleaning (CIP) implementation method: The cleaning process adopts the method of initial cleaning with lye and final cleaning with purified water. Among them, the initial cleaning duration of the lye is determined according to time, and cyclic lye initial cleaning (at this time, V116 is opened and V117 is closed) or discharged lye initial cleaning (at this time, V116 is closed and V117 is opened) can be realized according to the product characteristics. Usually, due to the relatively dirty fermentation tank 1 and the equipment at the front end of centrifugation, discharged lye initial cleaning is generally selected, and cyclic lye initial cleaning is adopted for the ultrafiltration at the back end. In this way, both the cleaning process can be satisfied and the consumption of lye can be saved. The final cleaning with purified water adopts the discharge cleaning method, that is, the conductivity of the purified water return water after cleaning is monitored in real time by the conductivity detection device 12. When the conductivity of the return water is less than the process set value, the final cleaning process with purified water ends.

[0045] When cleaning the fermentation tank 1: The valves V104, V105, V106, and V117 are opened, and the valves V101 and V116 are closed. The fermentation tank 1 is initially cleaned with lye by the method of discharged lye initial cleaning. Then, the fermentation tank 1 is cleaned by the method of final cleaning with purified water. During this process, if the conductivity detection device 12 monitors that the conductivity of the return water is greater than the process set value, then the valve V116 is opened and the valve V117 is closed. When the conductivity of the return water is less than the process set value, then the valve V116 is closed and the valve V117 is opened, and the final cleaning process with purified water ends.

[0046] When cleaning the first inlet pipe 5 and the disc centrifuge device 2: The valves V107, V101, V108, and V117 are opened, and the valves V102, V106, V110, and V116 are closed. The first inlet pipe 5 and the disc centrifuge device 2 are initially cleaned with lye by the method of discharged lye initial cleaning. Then, the same method as that for the fermentation tank 1 is adopted for its final cleaning with purified water.

[0047] When cleaning the second feed pipe 6: Open valves V109, V110, V102, V112 and V116, close valves V108, V106 and V117, and first perform an initial caustic wash on the second feed pipe 6 in a circulating caustic solution initial wash manner; after the initial caustic wash, perform a final wash with purified water.

[0048] When cleaning the first ultrafiltration device 3: Open valves V109, V111, V112 and V116, close valves V110, V106 and V117, and first perform an initial caustic wash on the first ultrafiltration device 3 in a circulating caustic solution initial wash manner; after the initial caustic wash, perform a final wash with purified water.

[0049] When cleaning the third feed pipe 7: Open valves V109, V113, V103 and V116, close valves V110, V111, V112 and V117, and first perform an initial caustic wash on the third feed pipe 7 in a circulating caustic solution initial wash manner; after the initial caustic wash, perform a final wash with purified water.

[0050] When cleaning the second ultrafiltration device 4: Open valves V109, V114, V115 and V116, close valves V110, V101, V113 and V117, and first perform an initial caustic wash on the second ultrafiltration device 4 in a circulating caustic solution initial wash manner; after the initial caustic wash, perform a final wash with purified water.

[0051] When discharging from the fermentation tank, open valves V105 and V101, close valve V106, the fermentation broth in the fermentation tank 1 directly enters the disc centrifuge device 2 through the first feed pipe 5, open V108 and the supernatant generated by centrifugation directly enters the first ultrafiltration device 3 through the second feed pipe 5, after the feed liquid passes through ultrafiltration, open valves V112 and V103 and enter the second ultrafiltration device 4 through the third feed pipe 7, after ultrafiltration is completed, collect the feed liquid at the concentration end.

[0052] The above are the preferred embodiments of the present invention, which are only used to explain the present invention and are not used to limit the present invention. Any obvious changes or variations derived from the technical solutions of the present invention are still within the protection scope of the present invention.

Claims

1. A processing system for continuous centrifugation and ultrafiltration of Escherichia coli fermentation broth, characterized in that, The processing system includes: A fermentation tank (1); A disc centrifuge device (2), which is connected and arranged downstream of the fermentation tank (1), and the fermentation broth in the fermentation tank (1) enters the disc centrifuge device (2) for centrifugation; A one-step ultrafiltration device (3), which is connected and arranged downstream of the disc centrifuge device (2), and the supernatant generated by centrifugation in the disc centrifuge device (2) enters the one-step ultrafiltration device (3) for ultrafiltration; A two-step ultrafiltration device (4), which is connected and arranged downstream of the one-step ultrafiltration device (3), and the liquid material after ultrafiltration in the one-step ultrafiltration device (3) enters the two-step ultrafiltration device (4) to continue ultrafiltration, and the concentrated liquid is collected after ultrafiltration; And a CIP on-line cleaning system, which is used to independently clean the fermentation tank (1), the disc centrifuge device (2) and its inlet pipeline, the one-step ultrafiltration device (3) and its inlet pipeline, the two-step ultrafiltration device (4) and its inlet pipeline respectively.

2. The continuous centrifugation and ultrafiltration treatment system for Escherichia coli fermentation broth according to claim 1, wherein The disc centrifuge device (2) is connected and arranged with the fermentation tank (1) through a first inlet pipe (5), and a valve V101 is arranged on the first inlet pipe (5).

3. A treatment system for continuous centrifugation and ultrafiltration of Escherichia coli fermentation broth according to claim 1, characterized in that, The one-step ultrafiltration device (3) is connected and arranged with the disc centrifuge device (2) through a second inlet pipe (6), and a valve V102 is arranged on the second inlet pipe (6).

4. A treatment system for continuous centrifugation and ultrafiltration of Escherichia coli fermentation broth according to claim 1, characterized in that, The two-step ultrafiltration device (4) is connected and arranged with the one-step ultrafiltration device (3) through a third inlet pipe (7), and a valve V103 is arranged on the third inlet pipe (7).

5. A treatment system for continuous centrifugation and ultrafiltration of Escherichia coli fermentation broth according to claim 1, characterized in that, The CIP on-line cleaning system includes: a CIP station (8) for providing cleaning liquid, a first circulation pump (9), a return water / discharge pipeline (10), a second circulation pump (11), a conductivity detection device (12), a first cleaning pipe (13) for independently cleaning the fermentation tank (1), a second cleaning pipe (14) for independently cleaning the first inlet pipe (5) and the disc centrifuge device (2), a third cleaning pipe (15) for independently cleaning the second inlet pipe (6), a fourth cleaning pipe (16) for independently cleaning the one-step ultrafiltration device (3), a fifth cleaning pipe (17) for independently cleaning the third inlet pipe (7), and a sixth cleaning pipe (18) for independently cleaning the two-step ultrafiltration device (4); The first circulation pump (9) is arranged at the outlet of the CIP station (8), the second circulation pump (11) and the conductivity detection device (12) are arranged on the return water / discharge pipeline (10), one end of the return water / discharge pipeline (10) is connected and arranged in parallel with the outlets of the fermentation tank (1), the disc centrifuge device (2), the one-step ultrafiltration device (3) and the two-step ultrafiltration device (4), and the other end is connected and arranged in parallel with the CIP station (8) and the discharge pipe (19), and the cleaned liquid flows back to the CIP station (8) or is discharged through the return water / discharge pipeline (10).

6. The continuous centrifugal ultrafiltration treatment system for Escherichia coli fermentation broth according to claim 5, wherein, The cleaning pipe 1 (13) connects the CIP station (8) and the fermenter (1), and a valve V104 is also provided thereon. A valve V105 and a valve V106 are provided on the return water / discharge pipeline (10) downstream near the outlet of the fermenter (1). One end of the feed pipe 1 (5) is connected to the return water / discharge pipeline (10) between the valve V105 and the valve V106, and the other end is connected to the disc centrifuge device (2).

7. A treatment system for continuous centrifugal ultrafiltration of Escherichia coli fermentation broth according to claim 5, characterized in that, One end of the cleaning pipe 2 (14) is connected to the CIP station (8), and the other end is connected to the feed pipe 1 (5) and near the connection of the feed pipe 1 (5) and the return water / discharge pipeline (10). A valve V107 is provided on the cleaning pipe 2 (14). A valve V108 is provided on the return water / discharge pipeline (10) near the outlet of the disc centrifuge device (2).

8. A processing system for continuous centrifugation and ultrafiltration of Escherichia coli fermentation broth according to claim 5, characterized in that, One end of the cleaning pipe 3 (15) is connected to the CIP station (8), and the other end is connected to the feed pipe 2 (6) and the connection is upstream of the valve V102. Valves V109 and V110 are provided on the cleaning pipe 3 (15). One end of the cleaning pipe 4 (16) is connected to the cleaning pipe 3 (15) between the valve V109 and the valve V110, and the other end is connected to the one-step ultrafiltration device (3). A valve V111 is provided on the cleaning pipe 4 (16). A valve V112 is also provided on the return water / discharge pipeline (10) near the outlet of the one-step ultrafiltration device (3).

9. The continuous centrifugation and ultrafiltration treatment system for Escherichia coli fermentation broth according to claim 8, wherein, One end of the cleaning pipe 5 (17) is connected to the cleaning pipe 4 (16) and the connection is upstream of the valve V111, and the other end is connected to the feed pipe 3 (7). A valve V113 is provided on the cleaning pipe 5 (17). One end of the cleaning pipe 6 (18) is connected to the cleaning pipe 5 (17) and the connection is upstream of the valve V113, and the other end is connected to the two-step ultrafiltration device (4). A valve V114 is provided on the cleaning pipe 6 (18). A valve V115 is also provided on the return water / discharge pipeline (10) near the outlet of the two-step ultrafiltration device (4).

10. A processing system for continuous centrifugation and ultrafiltration of Escherichia coli fermentation broth according to claim 5, characterized in that, A valve V116 is provided on the return water / discharge pipeline (10) near the inlet of the CIP station (8), and a valve V117 is provided on the discharge pipe (19).

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